magnetic streptavidin beads  (Vector Laboratories)


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    Vector Laboratories magnetic streptavidin beads
    Display of membrane skeleton-associated mannose patches is induced by oxidative stress and recognized by the mannose receptor on macrophages. a Peanut agglutinin (PNA) and Galanthus nivalis Agglutinin (GNA) lectin binding to normal hemoglobin (HbAA) RBCs with or without oxidation. Mannan blockade for GNA lectin binding shown in blue. 2 tailed Wilcoxon, paired data, PNA, n = 7; GNA, n = 8 biologically independent RBC donors over two independent experiments. b Immunofluorescence microscopy of GNA <t>lectin/streptavidin</t> (red) staining of healthy HbAA RBCs (above) and after the oxidative insult (below). c Normalized geometric mean fluorescence (gMFI) for binding analyzed by flow cytometry of murine Fc fusions with C-type lectins or sub-domains applied to oxidized versus undamaged RBCs. Mannan blockade of mannose receptor-carbohydrate recognition domain (MR-CRD) binding is shown in blue. MAH, macrophage antigen H. MR-CR, mannose receptor cysteine-rich domain. 2 tailed Wilcoxon, paired data, n = 7 biologically independent RBC donors over two independent experiments. d Immunofluorescence microscopy image of human monocyte-derived macrophages (HMDM) stained with DAPI (blue) and for mannose receptor (green) after incubation with oxidized HbAA RBCs, shown in magenta. e Percentage phagocytosis of oxidized RBCs by HMDM treated with human MR specific or scrambled siRNA. 2 tailed Mann–Whitney, n = 4 biologically independent RBC donors over two independent experiments. f Percentage phagocytosis of healthy or oxidized HbAA RBCs by HMDM with or without pre-blocking by mannan, chitin, or laminarin, 2 tailed Mann–Whitney, n = 4–8 biologically independent RBC donors over two independent experiments. No adjustments made for multiple comparisons. g As f but oxidized RBCs are blocked by MR-CRD blocking antibody 15.2 as indicated. 2 tailed Mann–Whitney, n = 6 biologically independent RBC donors over two independent experiments. h Percentage phagocytosis of sickle cell homozygote (HbSS) RBCs with or without pre-blocking by MR-CRD blocking antibody 15.2. 2 tailed Mann–Whitney, n = 5 biologically independent RBC donors over three independent experiments. i HbAA unblocked and HbSS unblocked or mannan and chitin blockade phagocytosis experiments as shown. 2 tailed Mann–Whitney, n = 4-7 biologically independent RBC donors. No adjustments made for multiple comparisons. All data are shown as median +/− IQR.
    Magnetic Streptavidin Beads, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/magnetic streptavidin beads/product/Vector Laboratories
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    magnetic streptavidin beads - by Bioz Stars, 2021-09
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    1) Product Images from "Red blood cell mannoses as phagocytic ligands mediating both sickle cell anaemia and malaria resistance"

    Article Title: Red blood cell mannoses as phagocytic ligands mediating both sickle cell anaemia and malaria resistance

    Journal: Nature Communications

    doi: 10.1038/s41467-021-21814-z

    Display of membrane skeleton-associated mannose patches is induced by oxidative stress and recognized by the mannose receptor on macrophages. a Peanut agglutinin (PNA) and Galanthus nivalis Agglutinin (GNA) lectin binding to normal hemoglobin (HbAA) RBCs with or without oxidation. Mannan blockade for GNA lectin binding shown in blue. 2 tailed Wilcoxon, paired data, PNA, n = 7; GNA, n = 8 biologically independent RBC donors over two independent experiments. b Immunofluorescence microscopy of GNA lectin/streptavidin (red) staining of healthy HbAA RBCs (above) and after the oxidative insult (below). c Normalized geometric mean fluorescence (gMFI) for binding analyzed by flow cytometry of murine Fc fusions with C-type lectins or sub-domains applied to oxidized versus undamaged RBCs. Mannan blockade of mannose receptor-carbohydrate recognition domain (MR-CRD) binding is shown in blue. MAH, macrophage antigen H. MR-CR, mannose receptor cysteine-rich domain. 2 tailed Wilcoxon, paired data, n = 7 biologically independent RBC donors over two independent experiments. d Immunofluorescence microscopy image of human monocyte-derived macrophages (HMDM) stained with DAPI (blue) and for mannose receptor (green) after incubation with oxidized HbAA RBCs, shown in magenta. e Percentage phagocytosis of oxidized RBCs by HMDM treated with human MR specific or scrambled siRNA. 2 tailed Mann–Whitney, n = 4 biologically independent RBC donors over two independent experiments. f Percentage phagocytosis of healthy or oxidized HbAA RBCs by HMDM with or without pre-blocking by mannan, chitin, or laminarin, 2 tailed Mann–Whitney, n = 4–8 biologically independent RBC donors over two independent experiments. No adjustments made for multiple comparisons. g As f but oxidized RBCs are blocked by MR-CRD blocking antibody 15.2 as indicated. 2 tailed Mann–Whitney, n = 6 biologically independent RBC donors over two independent experiments. h Percentage phagocytosis of sickle cell homozygote (HbSS) RBCs with or without pre-blocking by MR-CRD blocking antibody 15.2. 2 tailed Mann–Whitney, n = 5 biologically independent RBC donors over three independent experiments. i HbAA unblocked and HbSS unblocked or mannan and chitin blockade phagocytosis experiments as shown. 2 tailed Mann–Whitney, n = 4-7 biologically independent RBC donors. No adjustments made for multiple comparisons. All data are shown as median +/− IQR.
    Figure Legend Snippet: Display of membrane skeleton-associated mannose patches is induced by oxidative stress and recognized by the mannose receptor on macrophages. a Peanut agglutinin (PNA) and Galanthus nivalis Agglutinin (GNA) lectin binding to normal hemoglobin (HbAA) RBCs with or without oxidation. Mannan blockade for GNA lectin binding shown in blue. 2 tailed Wilcoxon, paired data, PNA, n = 7; GNA, n = 8 biologically independent RBC donors over two independent experiments. b Immunofluorescence microscopy of GNA lectin/streptavidin (red) staining of healthy HbAA RBCs (above) and after the oxidative insult (below). c Normalized geometric mean fluorescence (gMFI) for binding analyzed by flow cytometry of murine Fc fusions with C-type lectins or sub-domains applied to oxidized versus undamaged RBCs. Mannan blockade of mannose receptor-carbohydrate recognition domain (MR-CRD) binding is shown in blue. MAH, macrophage antigen H. MR-CR, mannose receptor cysteine-rich domain. 2 tailed Wilcoxon, paired data, n = 7 biologically independent RBC donors over two independent experiments. d Immunofluorescence microscopy image of human monocyte-derived macrophages (HMDM) stained with DAPI (blue) and for mannose receptor (green) after incubation with oxidized HbAA RBCs, shown in magenta. e Percentage phagocytosis of oxidized RBCs by HMDM treated with human MR specific or scrambled siRNA. 2 tailed Mann–Whitney, n = 4 biologically independent RBC donors over two independent experiments. f Percentage phagocytosis of healthy or oxidized HbAA RBCs by HMDM with or without pre-blocking by mannan, chitin, or laminarin, 2 tailed Mann–Whitney, n = 4–8 biologically independent RBC donors over two independent experiments. No adjustments made for multiple comparisons. g As f but oxidized RBCs are blocked by MR-CRD blocking antibody 15.2 as indicated. 2 tailed Mann–Whitney, n = 6 biologically independent RBC donors over two independent experiments. h Percentage phagocytosis of sickle cell homozygote (HbSS) RBCs with or without pre-blocking by MR-CRD blocking antibody 15.2. 2 tailed Mann–Whitney, n = 5 biologically independent RBC donors over three independent experiments. i HbAA unblocked and HbSS unblocked or mannan and chitin blockade phagocytosis experiments as shown. 2 tailed Mann–Whitney, n = 4-7 biologically independent RBC donors. No adjustments made for multiple comparisons. All data are shown as median +/− IQR.

    Techniques Used: Binding Assay, Immunofluorescence, Microscopy, Staining, Fluorescence, Flow Cytometry, Derivative Assay, Incubation, MANN-WHITNEY, Blocking Assay

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    Binding Assay:

    Article Title: Red blood cell mannoses as phagocytic ligands mediating both sickle cell anaemia and malaria resistance
    Article Snippet: .. Precipitation with magnetic streptavidin beads was performed in a binding buffer and the beads washed with a binding buffer containing 0.1% Triton X-100. ..

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    Vector Laboratories magnetic streptavidin beads
    Display of membrane skeleton-associated mannose patches is induced by oxidative stress and recognized by the mannose receptor on macrophages. a Peanut agglutinin (PNA) and Galanthus nivalis Agglutinin (GNA) lectin binding to normal hemoglobin (HbAA) RBCs with or without oxidation. Mannan blockade for GNA lectin binding shown in blue. 2 tailed Wilcoxon, paired data, PNA, n = 7; GNA, n = 8 biologically independent RBC donors over two independent experiments. b Immunofluorescence microscopy of GNA <t>lectin/streptavidin</t> (red) staining of healthy HbAA RBCs (above) and after the oxidative insult (below). c Normalized geometric mean fluorescence (gMFI) for binding analyzed by flow cytometry of murine Fc fusions with C-type lectins or sub-domains applied to oxidized versus undamaged RBCs. Mannan blockade of mannose receptor-carbohydrate recognition domain (MR-CRD) binding is shown in blue. MAH, macrophage antigen H. MR-CR, mannose receptor cysteine-rich domain. 2 tailed Wilcoxon, paired data, n = 7 biologically independent RBC donors over two independent experiments. d Immunofluorescence microscopy image of human monocyte-derived macrophages (HMDM) stained with DAPI (blue) and for mannose receptor (green) after incubation with oxidized HbAA RBCs, shown in magenta. e Percentage phagocytosis of oxidized RBCs by HMDM treated with human MR specific or scrambled siRNA. 2 tailed Mann–Whitney, n = 4 biologically independent RBC donors over two independent experiments. f Percentage phagocytosis of healthy or oxidized HbAA RBCs by HMDM with or without pre-blocking by mannan, chitin, or laminarin, 2 tailed Mann–Whitney, n = 4–8 biologically independent RBC donors over two independent experiments. No adjustments made for multiple comparisons. g As f but oxidized RBCs are blocked by MR-CRD blocking antibody 15.2 as indicated. 2 tailed Mann–Whitney, n = 6 biologically independent RBC donors over two independent experiments. h Percentage phagocytosis of sickle cell homozygote (HbSS) RBCs with or without pre-blocking by MR-CRD blocking antibody 15.2. 2 tailed Mann–Whitney, n = 5 biologically independent RBC donors over three independent experiments. i HbAA unblocked and HbSS unblocked or mannan and chitin blockade phagocytosis experiments as shown. 2 tailed Mann–Whitney, n = 4-7 biologically independent RBC donors. No adjustments made for multiple comparisons. All data are shown as median +/− IQR.
    Magnetic Streptavidin Beads, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/magnetic streptavidin beads/product/Vector Laboratories
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    magnetic streptavidin beads - by Bioz Stars, 2021-09
    94/100 stars
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    Display of membrane skeleton-associated mannose patches is induced by oxidative stress and recognized by the mannose receptor on macrophages. a Peanut agglutinin (PNA) and Galanthus nivalis Agglutinin (GNA) lectin binding to normal hemoglobin (HbAA) RBCs with or without oxidation. Mannan blockade for GNA lectin binding shown in blue. 2 tailed Wilcoxon, paired data, PNA, n = 7; GNA, n = 8 biologically independent RBC donors over two independent experiments. b Immunofluorescence microscopy of GNA lectin/streptavidin (red) staining of healthy HbAA RBCs (above) and after the oxidative insult (below). c Normalized geometric mean fluorescence (gMFI) for binding analyzed by flow cytometry of murine Fc fusions with C-type lectins or sub-domains applied to oxidized versus undamaged RBCs. Mannan blockade of mannose receptor-carbohydrate recognition domain (MR-CRD) binding is shown in blue. MAH, macrophage antigen H. MR-CR, mannose receptor cysteine-rich domain. 2 tailed Wilcoxon, paired data, n = 7 biologically independent RBC donors over two independent experiments. d Immunofluorescence microscopy image of human monocyte-derived macrophages (HMDM) stained with DAPI (blue) and for mannose receptor (green) after incubation with oxidized HbAA RBCs, shown in magenta. e Percentage phagocytosis of oxidized RBCs by HMDM treated with human MR specific or scrambled siRNA. 2 tailed Mann–Whitney, n = 4 biologically independent RBC donors over two independent experiments. f Percentage phagocytosis of healthy or oxidized HbAA RBCs by HMDM with or without pre-blocking by mannan, chitin, or laminarin, 2 tailed Mann–Whitney, n = 4–8 biologically independent RBC donors over two independent experiments. No adjustments made for multiple comparisons. g As f but oxidized RBCs are blocked by MR-CRD blocking antibody 15.2 as indicated. 2 tailed Mann–Whitney, n = 6 biologically independent RBC donors over two independent experiments. h Percentage phagocytosis of sickle cell homozygote (HbSS) RBCs with or without pre-blocking by MR-CRD blocking antibody 15.2. 2 tailed Mann–Whitney, n = 5 biologically independent RBC donors over three independent experiments. i HbAA unblocked and HbSS unblocked or mannan and chitin blockade phagocytosis experiments as shown. 2 tailed Mann–Whitney, n = 4-7 biologically independent RBC donors. No adjustments made for multiple comparisons. All data are shown as median +/− IQR.

    Journal: Nature Communications

    Article Title: Red blood cell mannoses as phagocytic ligands mediating both sickle cell anaemia and malaria resistance

    doi: 10.1038/s41467-021-21814-z

    Figure Lengend Snippet: Display of membrane skeleton-associated mannose patches is induced by oxidative stress and recognized by the mannose receptor on macrophages. a Peanut agglutinin (PNA) and Galanthus nivalis Agglutinin (GNA) lectin binding to normal hemoglobin (HbAA) RBCs with or without oxidation. Mannan blockade for GNA lectin binding shown in blue. 2 tailed Wilcoxon, paired data, PNA, n = 7; GNA, n = 8 biologically independent RBC donors over two independent experiments. b Immunofluorescence microscopy of GNA lectin/streptavidin (red) staining of healthy HbAA RBCs (above) and after the oxidative insult (below). c Normalized geometric mean fluorescence (gMFI) for binding analyzed by flow cytometry of murine Fc fusions with C-type lectins or sub-domains applied to oxidized versus undamaged RBCs. Mannan blockade of mannose receptor-carbohydrate recognition domain (MR-CRD) binding is shown in blue. MAH, macrophage antigen H. MR-CR, mannose receptor cysteine-rich domain. 2 tailed Wilcoxon, paired data, n = 7 biologically independent RBC donors over two independent experiments. d Immunofluorescence microscopy image of human monocyte-derived macrophages (HMDM) stained with DAPI (blue) and for mannose receptor (green) after incubation with oxidized HbAA RBCs, shown in magenta. e Percentage phagocytosis of oxidized RBCs by HMDM treated with human MR specific or scrambled siRNA. 2 tailed Mann–Whitney, n = 4 biologically independent RBC donors over two independent experiments. f Percentage phagocytosis of healthy or oxidized HbAA RBCs by HMDM with or without pre-blocking by mannan, chitin, or laminarin, 2 tailed Mann–Whitney, n = 4–8 biologically independent RBC donors over two independent experiments. No adjustments made for multiple comparisons. g As f but oxidized RBCs are blocked by MR-CRD blocking antibody 15.2 as indicated. 2 tailed Mann–Whitney, n = 6 biologically independent RBC donors over two independent experiments. h Percentage phagocytosis of sickle cell homozygote (HbSS) RBCs with or without pre-blocking by MR-CRD blocking antibody 15.2. 2 tailed Mann–Whitney, n = 5 biologically independent RBC donors over three independent experiments. i HbAA unblocked and HbSS unblocked or mannan and chitin blockade phagocytosis experiments as shown. 2 tailed Mann–Whitney, n = 4-7 biologically independent RBC donors. No adjustments made for multiple comparisons. All data are shown as median +/− IQR.

    Article Snippet: Precipitation with magnetic streptavidin beads was performed in a binding buffer and the beads washed with a binding buffer containing 0.1% Triton X-100.

    Techniques: Binding Assay, Immunofluorescence, Microscopy, Staining, Fluorescence, Flow Cytometry, Derivative Assay, Incubation, MANN-WHITNEY, Blocking Assay