pcis filters  (Worthington Biochemical)


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  • 85
    Name:
    Protease S Aureus Endoproteinase Glu C
    Description:
    Chromatographically purified according to Drapeau G Boily Y and Houmard J J Biol Chem 247 6720 1972 A lyophilized powder
    Catalog Number:
    ls003605
    Price:
    228
    Size:
    5 mg
    Source:
    S. aureus V8
    Cas Number:
    66676.43.5
    Buy from Supplier


    Structured Review

    Worthington Biochemical pcis filters
    Early formation of influenza virus-specific CPE in cells inoculated with material collected by a <t>PCIS</t> at air sampling site 3. (a) Noninfected ATCC <t>MDCK</t> cells (negative control), 3 days postseed. (b) ATCC MDCK cells inoculated with material scraped off the PCIS after-filter. (c) Noninfected MDCK-SIAT2,6-UF cells, (negative control), 3 days postseed. (d) MDCK-SIAT2,6-UF cells inoculated with material scraped off the PCIS after-filter.
    Chromatographically purified according to Drapeau G Boily Y and Houmard J J Biol Chem 247 6720 1972 A lyophilized powder
    https://www.bioz.com/result/pcis filters/product/Worthington Biochemical
    Average 85 stars, based on 1186 article reviews
    Price from $9.99 to $1999.99
    pcis filters - by Bioz Stars, 2020-05
    85/100 stars

    Images

    1) Product Images from "Detection and Isolation of Airborne Influenza A H3N2 Virus Using a Sioutas Personal Cascade Impactor Sampler"

    Article Title: Detection and Isolation of Airborne Influenza A H3N2 Virus Using a Sioutas Personal Cascade Impactor Sampler

    Journal: Influenza Research and Treatment

    doi: 10.1155/2013/656825

    Early formation of influenza virus-specific CPE in cells inoculated with material collected by a PCIS at air sampling site 3. (a) Noninfected ATCC MDCK cells (negative control), 3 days postseed. (b) ATCC MDCK cells inoculated with material scraped off the PCIS after-filter. (c) Noninfected MDCK-SIAT2,6-UF cells, (negative control), 3 days postseed. (d) MDCK-SIAT2,6-UF cells inoculated with material scraped off the PCIS after-filter.
    Figure Legend Snippet: Early formation of influenza virus-specific CPE in cells inoculated with material collected by a PCIS at air sampling site 3. (a) Noninfected ATCC MDCK cells (negative control), 3 days postseed. (b) ATCC MDCK cells inoculated with material scraped off the PCIS after-filter. (c) Noninfected MDCK-SIAT2,6-UF cells, (negative control), 3 days postseed. (d) MDCK-SIAT2,6-UF cells inoculated with material scraped off the PCIS after-filter.

    Techniques Used: Sampling, Negative Control

    2) Product Images from "Increasing phosphoproteomic coverage through sequential digestion by complementary proteases"

    Article Title: Increasing phosphoproteomic coverage through sequential digestion by complementary proteases

    Journal: Analytical and bioanalytical chemistry

    doi: 10.1007/s00216-011-5466-5

    Phosphorylation sites identified by each approach. (a) Total number of distinct phosphorylation sites by method. Each experimental approach identified a larger number of phosphorylation sites than Glu-C only workflows. (b) Comparison of unique phosphorylation
    Figure Legend Snippet: Phosphorylation sites identified by each approach. (a) Total number of distinct phosphorylation sites by method. Each experimental approach identified a larger number of phosphorylation sites than Glu-C only workflows. (b) Comparison of unique phosphorylation

    Techniques Used:

    Experimental design and analysis of sequential digestion workflows. (a) Schematic diagram of the three sequential digestion approaches. GSPT closely resembles the traditional workflow with substitution of Glu-C for initial digestion and trypsin digestion
    Figure Legend Snippet: Experimental design and analysis of sequential digestion workflows. (a) Schematic diagram of the three sequential digestion approaches. GSPT closely resembles the traditional workflow with substitution of Glu-C for initial digestion and trypsin digestion

    Techniques Used:

    Evaluation of a Glu-C only based phosphoproteomic workflow. (a) In the traditional phosphoproteomic workflow, proteins are obtained from cell lysates and proteolytically digested with trypsin. The resultant peptides are separated into fractions via strong
    Figure Legend Snippet: Evaluation of a Glu-C only based phosphoproteomic workflow. (a) In the traditional phosphoproteomic workflow, proteins are obtained from cell lysates and proteolytically digested with trypsin. The resultant peptides are separated into fractions via strong

    Techniques Used:

    3) Product Images from "NFATc1 Regulates Programmed Death-1 Expression Upon T Cell Activation 1"

    Article Title: NFATc1 Regulates Programmed Death-1 Expression Upon T Cell Activation 1

    Journal: Journal of immunology (Baltimore, Md. : 1950)

    doi:

    Two DNase I hypersensitive sites are located in 5′ conserved regions of the pdcd1 locus (A) The UCSC genome browser was used to identify regions of high DNA sequence conservation in the region 5′ of the PD-1 transcriptional start site (arrow). Two regions were identified and termed B and C (as seen labeled above with capital letters). (B) Nuclei from EL4 and A20 cells were isolated and treated with increasing concentrations of DNase I. Genomic DNA was obtained and digested with Hin dIII (H) to generate the full-length genomic DNA fragment (line below schematic). Digested DNA was then subjected to Southern blot analysis with a probe specific for the 3′ end of the Hin dIII fragment (box labeled in schematic). The resulting DNase I hypersensitive sites (DHS) map to conserved regions B and C located 5′ of the pdcd1 gene and are indicated by arrows.
    Figure Legend Snippet: Two DNase I hypersensitive sites are located in 5′ conserved regions of the pdcd1 locus (A) The UCSC genome browser was used to identify regions of high DNA sequence conservation in the region 5′ of the PD-1 transcriptional start site (arrow). Two regions were identified and termed B and C (as seen labeled above with capital letters). (B) Nuclei from EL4 and A20 cells were isolated and treated with increasing concentrations of DNase I. Genomic DNA was obtained and digested with Hin dIII (H) to generate the full-length genomic DNA fragment (line below schematic). Digested DNA was then subjected to Southern blot analysis with a probe specific for the 3′ end of the Hin dIII fragment (box labeled in schematic). The resulting DNase I hypersensitive sites (DHS) map to conserved regions B and C located 5′ of the pdcd1 gene and are indicated by arrows.

    Techniques Used: Sequencing, Labeling, Isolation, Southern Blot

    DNase I hypersensitive sites are located in the 5′ conserved region of the pdcd1 locus in primary CD8 T cells expressing PD-1 (A) A schematic of conserved regions of PD-1 and primer set amplicons are shown. (B) Nuclei from A20, EL4, and (C) primary CD8 T (+/- PMA/ionomycin) cells were isolated and treated with increasing concentrations of DNase I. Genomic DNA was isolated and subjected to real-time PCR analysis with primer sets (I, II, and III) spanning portions of the pdcd1 gene. Relative hypersensitivity was assayed by normalization of DNase I treated DNA to that of the genomic (untreated) DNA control and the data are plotted as fold sensitivity. Increasing DNase I concentration is indicated along the X-axis. The data shown are the average (-/+ standard error) of three independent experiments.
    Figure Legend Snippet: DNase I hypersensitive sites are located in the 5′ conserved region of the pdcd1 locus in primary CD8 T cells expressing PD-1 (A) A schematic of conserved regions of PD-1 and primer set amplicons are shown. (B) Nuclei from A20, EL4, and (C) primary CD8 T (+/- PMA/ionomycin) cells were isolated and treated with increasing concentrations of DNase I. Genomic DNA was isolated and subjected to real-time PCR analysis with primer sets (I, II, and III) spanning portions of the pdcd1 gene. Relative hypersensitivity was assayed by normalization of DNase I treated DNA to that of the genomic (untreated) DNA control and the data are plotted as fold sensitivity. Increasing DNase I concentration is indicated along the X-axis. The data shown are the average (-/+ standard error) of three independent experiments.

    Techniques Used: Expressing, Isolation, Real-time Polymerase Chain Reaction, Concentration Assay

    4) Product Images from "Increasing phosphoproteomic coverage through sequential digestion by complementary proteases"

    Article Title: Increasing phosphoproteomic coverage through sequential digestion by complementary proteases

    Journal: Analytical and bioanalytical chemistry

    doi: 10.1007/s00216-011-5466-5

    Phosphorylation sites identified by each approach. (a) Total number of distinct phosphorylation sites by method. Each experimental approach identified a larger number of phosphorylation sites than Glu-C only workflows. (b) Comparison of unique phosphorylation
    Figure Legend Snippet: Phosphorylation sites identified by each approach. (a) Total number of distinct phosphorylation sites by method. Each experimental approach identified a larger number of phosphorylation sites than Glu-C only workflows. (b) Comparison of unique phosphorylation

    Techniques Used:

    Experimental design and analysis of sequential digestion workflows. (a) Schematic diagram of the three sequential digestion approaches. GSPT closely resembles the traditional workflow with substitution of Glu-C for initial digestion and trypsin digestion
    Figure Legend Snippet: Experimental design and analysis of sequential digestion workflows. (a) Schematic diagram of the three sequential digestion approaches. GSPT closely resembles the traditional workflow with substitution of Glu-C for initial digestion and trypsin digestion

    Techniques Used:

    Evaluation of a Glu-C only based phosphoproteomic workflow. (a) In the traditional phosphoproteomic workflow, proteins are obtained from cell lysates and proteolytically digested with trypsin. The resultant peptides are separated into fractions via strong
    Figure Legend Snippet: Evaluation of a Glu-C only based phosphoproteomic workflow. (a) In the traditional phosphoproteomic workflow, proteins are obtained from cell lysates and proteolytically digested with trypsin. The resultant peptides are separated into fractions via strong

    Techniques Used:

    5) Product Images from "Detection and Isolation of Airborne Influenza A H3N2 Virus Using a Sioutas Personal Cascade Impactor Sampler"

    Article Title: Detection and Isolation of Airborne Influenza A H3N2 Virus Using a Sioutas Personal Cascade Impactor Sampler

    Journal: Influenza Research and Treatment

    doi: 10.1155/2013/656825

    Early formation of influenza virus-specific CPE in cells inoculated with material collected by a PCIS at air sampling site 3. (a) Noninfected ATCC MDCK cells (negative control), 3 days postseed. (b) ATCC MDCK cells inoculated with material scraped off the PCIS after-filter. (c) Noninfected MDCK-SIAT2,6-UF cells, (negative control), 3 days postseed. (d) MDCK-SIAT2,6-UF cells inoculated with material scraped off the PCIS after-filter.
    Figure Legend Snippet: Early formation of influenza virus-specific CPE in cells inoculated with material collected by a PCIS at air sampling site 3. (a) Noninfected ATCC MDCK cells (negative control), 3 days postseed. (b) ATCC MDCK cells inoculated with material scraped off the PCIS after-filter. (c) Noninfected MDCK-SIAT2,6-UF cells, (negative control), 3 days postseed. (d) MDCK-SIAT2,6-UF cells inoculated with material scraped off the PCIS after-filter.

    Techniques Used: Sampling, Negative Control

    6) Product Images from "Detection and Isolation of Airborne Influenza A H3N2 Virus Using a Sioutas Personal Cascade Impactor Sampler"

    Article Title: Detection and Isolation of Airborne Influenza A H3N2 Virus Using a Sioutas Personal Cascade Impactor Sampler

    Journal: Influenza Research and Treatment

    doi: 10.1155/2013/656825

    Early formation of influenza virus-specific CPE in cells inoculated with material collected by a PCIS at air sampling site 3. (a) Noninfected ATCC MDCK cells (negative control), 3 days postseed. (b) ATCC MDCK cells inoculated with material scraped off the PCIS after-filter. (c) Noninfected MDCK-SIAT2,6-UF cells, (negative control), 3 days postseed. (d) MDCK-SIAT2,6-UF cells inoculated with material scraped off the PCIS after-filter.
    Figure Legend Snippet: Early formation of influenza virus-specific CPE in cells inoculated with material collected by a PCIS at air sampling site 3. (a) Noninfected ATCC MDCK cells (negative control), 3 days postseed. (b) ATCC MDCK cells inoculated with material scraped off the PCIS after-filter. (c) Noninfected MDCK-SIAT2,6-UF cells, (negative control), 3 days postseed. (d) MDCK-SIAT2,6-UF cells inoculated with material scraped off the PCIS after-filter.

    Techniques Used: Sampling, Negative Control

    Related Articles

    Modification:

    Article Title: Increasing phosphoproteomic coverage through sequential digestion by complementary proteases
    Article Snippet: .. Modified trypsin was from Promega (Madison, WI); Glu-C protease was from Worthington Biochemicals (Lakewood, NJ). .. Urea, Tris-HCl, CaCl2 , ammonium bicarbonate (NH4 HCO3 ), sodium fluoride (NaCl), potassium fluoride (KCl), potassium phosphate (KH2 PO4 ), phosphoric acid, sodium ortho-vanadate, sodium molybdate, sodium tartrate, beta-glycerophosphate, DL-dithiothreitol, iodoacetamide were from Sigma-Aldrich (St. Louis, MO).

    Immunoprecipitation:

    Article Title: Identification of a region in the coiled-coil domain of Smc3 that is essential for cohesin activity
    Article Snippet: .. Limited proteolysis Immunoprecipitated Smc3-3V5, either wild-type or L217P, were incubated in 20 μl of buffer (50 mM Tris–HCl (pH 8.0) and 1 mM MgCl2 ) with 5 mg/ml V8 protease (Worthington Biochemical), with or without 1 mM ATP (Sigma). ..

    Incubation:

    Article Title: Identification of a region in the coiled-coil domain of Smc3 that is essential for cohesin activity
    Article Snippet: .. Smc3-3V5 or smc3-L217P-3V5 were precipitated from asynchronous cell cultures and incubated with V8 protease, with or without ATP. .. The reaction products were analyzed by western blot with antibodies against V5.

    Article Title: Identification of a region in the coiled-coil domain of Smc3 that is essential for cohesin activity
    Article Snippet: .. Limited proteolysis Immunoprecipitated Smc3-3V5, either wild-type or L217P, were incubated in 20 μl of buffer (50 mM Tris–HCl (pH 8.0) and 1 mM MgCl2 ) with 5 mg/ml V8 protease (Worthington Biochemical), with or without 1 mM ATP (Sigma). ..

    other:

    Article Title: Increasing phosphoproteomic coverage through sequential digestion by complementary proteases
    Article Snippet: Briefly, a single, large pool of NCI-H23 cells were lysed in 8M urea, and their proteins were digested using Glu-C protease, desalted, and lyophilized in three equal (5mg) aliquots.

    Article Title: Detection of Protein Modifications and Counterfeit Protein Pharmaceuticals Using iTRAQ and MALDI TOF/TOF Mass Spectrometry: Studies with Insulins
    Article Snippet: S. aureus V8 (endoprotease Glu-C) and TLCK treated chymotrypsin were obtained from Worthington Biochemical (Lakewood, NJ).

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