ldh (Worthington Biochemical)

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Name:

Lactate Dehydrogenase Lyophilized

Description:

Chromatographically purified A lyophilized powder

Catalog Number:

ls002755

Price:

105

Size:

5 ku

Source:

Rabbit Muscle

Cas Number:

9001.60.9

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Structured Review

Worthington Biochemical ldh
Chromatographically purified A lyophilized powder
https://www.bioz.com/result/ldh/product/Worthington Biochemical
Average 90 stars, based on 1 article reviews
Price from $9.99 to $1999.99

ldh - by Bioz Stars, 2020-10

90/100 stars

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Images

Concentration Assay:

Article Title: High-Throughput Hyperpolarized 13C Metabolic Investigations using a Multi-Channel Acquisition System
Article Snippet: .. Four 300 μL wells of a standard 96-well plate were filled with an enzyme mixture containing 4.6 mM β-NADH (Sigma Aldrich), a variable concentration (0.0, 1.0, 3.0, and 9.0 U/mL) of lactate dehydrogenase (LDH) (Worthington Biochemical Corp., Lakewood, NJ), and 4 mM final concentration of HP [1-13 C] pyruvate in a buffer solution containing 203.3 mM NaCl and 81.3 mM Tris (7.6 pH preset). ..

other:

Article Title: Separate and Combined Biochemical Activities of the Subunits of a Naturally Split Reverse Gyrase *
Article Snippet: To calibrate the column, the following proteins were used as column standards: thyroglobulin (with a Stokes radius of 86 Å, Sigma); β-galactosidase (69 Å, Boehringer Mannhelm); type I horse spleen ferritin (63 Å, Sigma); lactate dehydrogenase (41 Å, Worthington); and bovine serum albumin (35Å, Pentex).

Article Title: Effects of anti-oestrogens and ?-estradiol on calcium uptake by cardiac sarcoplasmic reticulum
Article Snippet: Lactate dehydrogenase was purchased from Worthington (Freehold, NJ, U.S.A.).

Article Title: Evaluation of Hyperpolarized [1-13C]-Pyruvate by Magnetic Resonance to Detect Ionizing Radiation Effects in Real Time
Article Snippet: The combination nominally included 2 mM hyperpolarized 13 C-Pyruvate, 40 mM Lactate, and 3.92 U/mL Lactate Dehydrogenase (Worthington Biochemical Corp., Lakewood, NJ) in a Tris buffer (81.3 mM Trisma preset crystals pH 7.2, 203.3 mM NaCl) (Sigma-Aldrich, St. Louis, MO).

Article Title: Nuclear protein synthesis: A re-evaluation
Article Snippet: Activities of lactate dehydrogenase and glyceraldehyde-3-phosphate dehydrogenase were determined as described in the Worthington Biochemical Corporation Enzyme Manual (1972).

Article Title: A Catalyzing Phantom for Reproducible Dynamic Conversion of Hyperpolarized [1-13C]-Pyruvate
Article Snippet: Nominal final concentrations were 2 mM hyperpolarized 13 C-Pyruvate, 40 mM Lactate, 3.92 U/mL LDH (Worthington Biochemical Corp., Lakewood, NJ), and 4 mM Β-NADH (Sigma-Aldrich Corp., St. Louis, MO) in the Tris buffer (81.3 mM trisma preset crystals pH 7.2, 203.3 mM NaCl) (Sigma-Aldrich).

Purification:

Article Title: Cytosolic NADH-NAD+ Redox Visualized in Brain Slices by Two-Photon Fluorescence Lifetime Biosensor Imaging
Article Snippet: .. Different ratios of lactate/pyruvate were used to produce different NAD+ /NADH ratios by adding purified LDH (5 U/mL; Worthington Biochemical Corp., Lakewood, NJ). .. Protein solutions were loaded into borosilicate capillary glass (Warner Instruments, Hamden, CT) pulled to a fine taper and fire-polished until sealed.