laao solution  (Worthington Biochemical)

Journal: PLoS ONE

Article Title: A Highly Sensitive Method for Quantitative Determination of L-Amino Acid Oxidase Activity Based on the Visualization of Ferric-Xylenol Orange Formation

doi: 10.1371/journal.pone.0082483

Figure Lengend Snippet: Characterization of LAAO activities from Pseudoalteromonas sp. R3 (R3-LAAO) with L-Leu and L-Met as substrates, respectively (A) and from Crotalus adamanteus venom (caLAAO) with L-Leu as substrate (B) based on the Fe III XO agar assay. On the basis of the diameters of the formed purplish red Fe III XO halos, the concentrations of H 2 O 2 produced by LAAO activities were calculated with the equations in Figure 2 . The corresponding standard H 2 O 2 solutions as indicated above the corresponding holes were used to confirm the accuracy of Fe III XO agar assay. The diameters of the purplish red halos were marked below the holes. Without R3-LAAO, both L-Leu and L-Met (negative controls) did not give purplish red Fe III XO halos (right holes).

Article Snippet: LAAO solution from Crotalus adamanteus venom (caLAAO) was purchased from Worthington Biochemical Corporation, USA.

Techniques: Produced

Journal: PLoS ONE

Article Title: A Highly Sensitive Method for Quantitative Determination of L-Amino Acid Oxidase Activity Based on the Visualization of Ferric-Xylenol Orange Formation

doi: 10.1371/journal.pone.0082483

Figure Lengend Snippet: Dependence of detection solution pH for detection of standard H 2 O 2 or H 2 O 2 caused by LAAO activity in Fe III XO agar assay. Row 1: 14 background solutions with different pH values from 1 to 14 (from left to right) prepared by a mixture of 6 N HCl and 6 N NaOH; row 2: 14 standard 20 µM H 2 O 2 solutions with different pH values ranging from 1 to 14 (from left to right) adjusted by either HCl or NaOH; row 3: 14 standard 20 µM H 2 O 2 solutions with uniform pH of 7.5; row 4: 14 individual oxidization reactions of L-Leu by LAAO from Psudoalteromonas sp. R3 (R3-LAAO) with different pH values from 1 to 14 (from left to right) adjusted by either HCl or NaOH after oxidation; row 5: 14 individual oxidization reactions of L-Leu by R3-LAAO with uniform pH of 7.5.

Article Snippet: Stereospecific oxidation of amino acid by LAAO activity The stereospecific oxidation reaction was performed with 10 mM of each amino acid in 10 mL of R3-LAAO solution harvested from Psudoalteromonas sp. R3 culture supernatant or caLAAO solution (mixture of 1 µL caLAAO with 10 mL of 0.1 M PBS buffer with pH 7.5, Worthington Biochemical Corporation, USA).

Techniques: Activity Assay

Journal: PLoS ONE

Article Title: A Highly Sensitive Method for Quantitative Determination of L-Amino Acid Oxidase Activity Based on the Visualization of Ferric-Xylenol Orange Formation

doi: 10.1371/journal.pone.0082483

Figure Lengend Snippet: Characterization of LAAO activities from Pseudoalteromonas sp. R3 (R3-LAAO) with L-Leu and L-Met as substrates, respectively (A) and from Crotalus adamanteus venom (caLAAO) with L-Leu as substrate (B) based on the Fe III XO agar assay. On the basis of the diameters of the formed purplish red Fe III XO halos, the concentrations of H 2 O 2 produced by LAAO activities were calculated with the equations in Figure 2 . The corresponding standard H 2 O 2 solutions as indicated above the corresponding holes were used to confirm the accuracy of Fe III XO agar assay. The diameters of the purplish red halos were marked below the holes. Without R3-LAAO, both L-Leu and L-Met (negative controls) did not give purplish red Fe III XO halos (right holes).

Article Snippet: Stereospecific oxidation of amino acid by LAAO activity The stereospecific oxidation reaction was performed with 10 mM of each amino acid in 10 mL of R3-LAAO solution harvested from Psudoalteromonas sp. R3 culture supernatant or caLAAO solution (mixture of 1 µL caLAAO with 10 mL of 0.1 M PBS buffer with pH 7.5, Worthington Biochemical Corporation, USA).

Techniques: Produced