laao solution  (Worthington Biochemical)


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  • 85
    Name:
    Amino Acid Oxidase L
    Description:
    An aqueous solution with toluene added as a preservative
    Catalog Number:
    ls002763
    Price:
    74
    Size:
    2 mg
    Source:
    Crotalus adamanteus Venom
    Cas Number:
    9000.89.9
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    Structured Review

    Worthington Biochemical laao solution
    Characterization of <t>LAAO</t> activities from Pseudoalteromonas sp. R3 (R3-LAAO) with L-Leu and L-Met as substrates, respectively (A) and from Crotalus <t>adamanteus</t> venom (caLAAO) with L-Leu as substrate (B) based on the Fe III XO agar assay. On the basis of the diameters of the formed purplish red Fe III XO halos, the concentrations of H 2 O 2 produced by LAAO activities were calculated with the equations in Figure 2 . The corresponding standard H 2 O 2 solutions as indicated above the corresponding holes were used to confirm the accuracy of Fe III XO agar assay. The diameters of the purplish red halos were marked below the holes. Without R3-LAAO, both L-Leu and L-Met (negative controls) did not give purplish red Fe III XO halos (right holes).
    An aqueous solution with toluene added as a preservative
    https://www.bioz.com/result/laao solution/product/Worthington Biochemical
    Average 85 stars, based on 5 article reviews
    Price from $9.99 to $1999.99
    laao solution - by Bioz Stars, 2020-08
    85/100 stars

    Images

    1) Product Images from "A Highly Sensitive Method for Quantitative Determination of L-Amino Acid Oxidase Activity Based on the Visualization of Ferric-Xylenol Orange Formation"

    Article Title: A Highly Sensitive Method for Quantitative Determination of L-Amino Acid Oxidase Activity Based on the Visualization of Ferric-Xylenol Orange Formation

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0082483

    Characterization of LAAO activities from Pseudoalteromonas sp. R3 (R3-LAAO) with L-Leu and L-Met as substrates, respectively (A) and from Crotalus adamanteus venom (caLAAO) with L-Leu as substrate (B) based on the Fe III XO agar assay. On the basis of the diameters of the formed purplish red Fe III XO halos, the concentrations of H 2 O 2 produced by LAAO activities were calculated with the equations in Figure 2 . The corresponding standard H 2 O 2 solutions as indicated above the corresponding holes were used to confirm the accuracy of Fe III XO agar assay. The diameters of the purplish red halos were marked below the holes. Without R3-LAAO, both L-Leu and L-Met (negative controls) did not give purplish red Fe III XO halos (right holes).
    Figure Legend Snippet: Characterization of LAAO activities from Pseudoalteromonas sp. R3 (R3-LAAO) with L-Leu and L-Met as substrates, respectively (A) and from Crotalus adamanteus venom (caLAAO) with L-Leu as substrate (B) based on the Fe III XO agar assay. On the basis of the diameters of the formed purplish red Fe III XO halos, the concentrations of H 2 O 2 produced by LAAO activities were calculated with the equations in Figure 2 . The corresponding standard H 2 O 2 solutions as indicated above the corresponding holes were used to confirm the accuracy of Fe III XO agar assay. The diameters of the purplish red halos were marked below the holes. Without R3-LAAO, both L-Leu and L-Met (negative controls) did not give purplish red Fe III XO halos (right holes).

    Techniques Used: Produced

    2) Product Images from "A Highly Sensitive Method for Quantitative Determination of L-Amino Acid Oxidase Activity Based on the Visualization of Ferric-Xylenol Orange Formation"

    Article Title: A Highly Sensitive Method for Quantitative Determination of L-Amino Acid Oxidase Activity Based on the Visualization of Ferric-Xylenol Orange Formation

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0082483

    Dependence of detection solution pH for detection of standard H 2 O 2 or H 2 O 2 caused by LAAO activity in Fe III XO agar assay. Row 1: 14 background solutions with different pH values from 1 to 14 (from left to right) prepared by a mixture of 6 N HCl and 6 N NaOH; row 2: 14 standard 20 µM H 2 O 2 solutions with different pH values ranging from 1 to 14 (from left to right) adjusted by either HCl or NaOH; row 3: 14 standard 20 µM H 2 O 2 solutions with uniform pH of 7.5; row 4: 14 individual oxidization reactions of L-Leu by LAAO from Psudoalteromonas sp. R3 (R3-LAAO) with different pH values from 1 to 14 (from left to right) adjusted by either HCl or NaOH after oxidation; row 5: 14 individual oxidization reactions of L-Leu by R3-LAAO with uniform pH of 7.5.
    Figure Legend Snippet: Dependence of detection solution pH for detection of standard H 2 O 2 or H 2 O 2 caused by LAAO activity in Fe III XO agar assay. Row 1: 14 background solutions with different pH values from 1 to 14 (from left to right) prepared by a mixture of 6 N HCl and 6 N NaOH; row 2: 14 standard 20 µM H 2 O 2 solutions with different pH values ranging from 1 to 14 (from left to right) adjusted by either HCl or NaOH; row 3: 14 standard 20 µM H 2 O 2 solutions with uniform pH of 7.5; row 4: 14 individual oxidization reactions of L-Leu by LAAO from Psudoalteromonas sp. R3 (R3-LAAO) with different pH values from 1 to 14 (from left to right) adjusted by either HCl or NaOH after oxidation; row 5: 14 individual oxidization reactions of L-Leu by R3-LAAO with uniform pH of 7.5.

    Techniques Used: Activity Assay

    Characterization of LAAO activities from Pseudoalteromonas sp. R3 (R3-LAAO) with L-Leu and L-Met as substrates, respectively (A) and from Crotalus adamanteus venom (caLAAO) with L-Leu as substrate (B) based on the Fe III XO agar assay. On the basis of the diameters of the formed purplish red Fe III XO halos, the concentrations of H 2 O 2 produced by LAAO activities were calculated with the equations in Figure 2 . The corresponding standard H 2 O 2 solutions as indicated above the corresponding holes were used to confirm the accuracy of Fe III XO agar assay. The diameters of the purplish red halos were marked below the holes. Without R3-LAAO, both L-Leu and L-Met (negative controls) did not give purplish red Fe III XO halos (right holes).
    Figure Legend Snippet: Characterization of LAAO activities from Pseudoalteromonas sp. R3 (R3-LAAO) with L-Leu and L-Met as substrates, respectively (A) and from Crotalus adamanteus venom (caLAAO) with L-Leu as substrate (B) based on the Fe III XO agar assay. On the basis of the diameters of the formed purplish red Fe III XO halos, the concentrations of H 2 O 2 produced by LAAO activities were calculated with the equations in Figure 2 . The corresponding standard H 2 O 2 solutions as indicated above the corresponding holes were used to confirm the accuracy of Fe III XO agar assay. The diameters of the purplish red halos were marked below the holes. Without R3-LAAO, both L-Leu and L-Met (negative controls) did not give purplish red Fe III XO halos (right holes).

    Techniques Used: Produced

    Related Articles

    other:

    Article Title: Enzymatic measurement of phosphatidylserine in cultured cells
    Article Snippet: L-Amino acid oxidase (LAAO) from Crotalus adamanteus venom was obtained from Worthington (Lakewood, NJ).

    Article Title: Host Cell Plasma Membrane Phosphatidylserine Regulates the Assembly and Budding of Ebola Virus
    Article Snippet: Glutathione S -transferase GST resin (Novagen, San Diego, CA), thrombin (GE Healthcare, Pittsburgh, PA), HisTag resin (Qiagen), L1 sensor chips (GE Healthcare), VP40 antibody (IBT Bioservices, Gaithersburg, MD), secondary goat anti-rabbit horseradish peroxidase (HRP) conjugate (Bio-Rad, Hercules, CA), anti-phosphatidylserine [4B6] antibody (Abcam, Cambridge, MA), and l -amino acid oxidase (LAAO) (from Crotalus adamanteus venom; Worthington, Lakewood, NJ) were obtained from the indicated sources.

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  • 88
    Worthington Biochemical l amino acid oxidase laao
    L Amino Acid Oxidase Laao, supplied by Worthington Biochemical, used in various techniques. Bioz Stars score: 88/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/l amino acid oxidase laao/product/Worthington Biochemical
    Average 88 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    l amino acid oxidase laao - by Bioz Stars, 2020-08
    88/100 stars
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    85
    Worthington Biochemical laao solution
    Characterization of <t>LAAO</t> activities from Pseudoalteromonas sp. R3 (R3-LAAO) with L-Leu and L-Met as substrates, respectively (A) and from Crotalus <t>adamanteus</t> venom (caLAAO) with L-Leu as substrate (B) based on the Fe III XO agar assay. On the basis of the diameters of the formed purplish red Fe III XO halos, the concentrations of H 2 O 2 produced by LAAO activities were calculated with the equations in Figure 2 . The corresponding standard H 2 O 2 solutions as indicated above the corresponding holes were used to confirm the accuracy of Fe III XO agar assay. The diameters of the purplish red halos were marked below the holes. Without R3-LAAO, both L-Leu and L-Met (negative controls) did not give purplish red Fe III XO halos (right holes).
    Laao Solution, supplied by Worthington Biochemical, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/laao solution/product/Worthington Biochemical
    Average 85 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    laao solution - by Bioz Stars, 2020-08
    85/100 stars
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    85
    Worthington Biochemical r3 laao solution
    Dependence of detection solution pH for detection of standard H 2 O 2 or H 2 O 2 caused by <t>LAAO</t> activity in Fe III XO agar assay. Row 1: 14 background solutions with different pH values from 1 to 14 (from left to right) prepared by a mixture of 6 N HCl and 6 N NaOH; row 2: 14 standard 20 µM H 2 O 2 solutions with different pH values ranging from 1 to 14 (from left to right) adjusted by either HCl or NaOH; row 3: 14 standard 20 µM H 2 O 2 solutions with uniform pH of 7.5; row 4: 14 individual oxidization reactions of L-Leu by LAAO from Psudoalteromonas sp. R3 <t>(R3-LAAO)</t> with different pH values from 1 to 14 (from left to right) adjusted by either HCl or NaOH after oxidation; row 5: 14 individual oxidization reactions of L-Leu by R3-LAAO with uniform pH of 7.5.
    R3 Laao Solution, supplied by Worthington Biochemical, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/r3 laao solution/product/Worthington Biochemical
    Average 85 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    r3 laao solution - by Bioz Stars, 2020-08
    85/100 stars
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    85
    Worthington Biochemical laao activity
    Dependence of detection solution pH for detection of standard H 2 O 2 or H 2 O 2 caused by <t>LAAO</t> activity in Fe III XO agar assay. Row 1: 14 background solutions with different pH values from 1 to 14 (from left to right) prepared by a mixture of 6 N HCl and 6 N NaOH; row 2: 14 standard 20 µM H 2 O 2 solutions with different pH values ranging from 1 to 14 (from left to right) adjusted by either HCl or NaOH; row 3: 14 standard 20 µM H 2 O 2 solutions with uniform pH of 7.5; row 4: 14 individual oxidization reactions of L-Leu by LAAO from Psudoalteromonas sp. R3 <t>(R3-LAAO)</t> with different pH values from 1 to 14 (from left to right) adjusted by either HCl or NaOH after oxidation; row 5: 14 individual oxidization reactions of L-Leu by R3-LAAO with uniform pH of 7.5.
    Laao Activity, supplied by Worthington Biochemical, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/laao activity/product/Worthington Biochemical
    Average 85 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    laao activity - by Bioz Stars, 2020-08
    85/100 stars
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    Image Search Results


    Characterization of LAAO activities from Pseudoalteromonas sp. R3 (R3-LAAO) with L-Leu and L-Met as substrates, respectively (A) and from Crotalus adamanteus venom (caLAAO) with L-Leu as substrate (B) based on the Fe III XO agar assay. On the basis of the diameters of the formed purplish red Fe III XO halos, the concentrations of H 2 O 2 produced by LAAO activities were calculated with the equations in Figure 2 . The corresponding standard H 2 O 2 solutions as indicated above the corresponding holes were used to confirm the accuracy of Fe III XO agar assay. The diameters of the purplish red halos were marked below the holes. Without R3-LAAO, both L-Leu and L-Met (negative controls) did not give purplish red Fe III XO halos (right holes).

    Journal: PLoS ONE

    Article Title: A Highly Sensitive Method for Quantitative Determination of L-Amino Acid Oxidase Activity Based on the Visualization of Ferric-Xylenol Orange Formation

    doi: 10.1371/journal.pone.0082483

    Figure Lengend Snippet: Characterization of LAAO activities from Pseudoalteromonas sp. R3 (R3-LAAO) with L-Leu and L-Met as substrates, respectively (A) and from Crotalus adamanteus venom (caLAAO) with L-Leu as substrate (B) based on the Fe III XO agar assay. On the basis of the diameters of the formed purplish red Fe III XO halos, the concentrations of H 2 O 2 produced by LAAO activities were calculated with the equations in Figure 2 . The corresponding standard H 2 O 2 solutions as indicated above the corresponding holes were used to confirm the accuracy of Fe III XO agar assay. The diameters of the purplish red halos were marked below the holes. Without R3-LAAO, both L-Leu and L-Met (negative controls) did not give purplish red Fe III XO halos (right holes).

    Article Snippet: LAAO solution from Crotalus adamanteus venom (caLAAO) was purchased from Worthington Biochemical Corporation, USA.

    Techniques: Produced

    Dependence of detection solution pH for detection of standard H 2 O 2 or H 2 O 2 caused by LAAO activity in Fe III XO agar assay. Row 1: 14 background solutions with different pH values from 1 to 14 (from left to right) prepared by a mixture of 6 N HCl and 6 N NaOH; row 2: 14 standard 20 µM H 2 O 2 solutions with different pH values ranging from 1 to 14 (from left to right) adjusted by either HCl or NaOH; row 3: 14 standard 20 µM H 2 O 2 solutions with uniform pH of 7.5; row 4: 14 individual oxidization reactions of L-Leu by LAAO from Psudoalteromonas sp. R3 (R3-LAAO) with different pH values from 1 to 14 (from left to right) adjusted by either HCl or NaOH after oxidation; row 5: 14 individual oxidization reactions of L-Leu by R3-LAAO with uniform pH of 7.5.

    Journal: PLoS ONE

    Article Title: A Highly Sensitive Method for Quantitative Determination of L-Amino Acid Oxidase Activity Based on the Visualization of Ferric-Xylenol Orange Formation

    doi: 10.1371/journal.pone.0082483

    Figure Lengend Snippet: Dependence of detection solution pH for detection of standard H 2 O 2 or H 2 O 2 caused by LAAO activity in Fe III XO agar assay. Row 1: 14 background solutions with different pH values from 1 to 14 (from left to right) prepared by a mixture of 6 N HCl and 6 N NaOH; row 2: 14 standard 20 µM H 2 O 2 solutions with different pH values ranging from 1 to 14 (from left to right) adjusted by either HCl or NaOH; row 3: 14 standard 20 µM H 2 O 2 solutions with uniform pH of 7.5; row 4: 14 individual oxidization reactions of L-Leu by LAAO from Psudoalteromonas sp. R3 (R3-LAAO) with different pH values from 1 to 14 (from left to right) adjusted by either HCl or NaOH after oxidation; row 5: 14 individual oxidization reactions of L-Leu by R3-LAAO with uniform pH of 7.5.

    Article Snippet: Stereospecific oxidation of amino acid by LAAO activity The stereospecific oxidation reaction was performed with 10 mM of each amino acid in 10 mL of R3-LAAO solution harvested from Psudoalteromonas sp. R3 culture supernatant or caLAAO solution (mixture of 1 µL caLAAO with 10 mL of 0.1 M PBS buffer with pH 7.5, Worthington Biochemical Corporation, USA).

    Techniques: Activity Assay

    Characterization of LAAO activities from Pseudoalteromonas sp. R3 (R3-LAAO) with L-Leu and L-Met as substrates, respectively (A) and from Crotalus adamanteus venom (caLAAO) with L-Leu as substrate (B) based on the Fe III XO agar assay. On the basis of the diameters of the formed purplish red Fe III XO halos, the concentrations of H 2 O 2 produced by LAAO activities were calculated with the equations in Figure 2 . The corresponding standard H 2 O 2 solutions as indicated above the corresponding holes were used to confirm the accuracy of Fe III XO agar assay. The diameters of the purplish red halos were marked below the holes. Without R3-LAAO, both L-Leu and L-Met (negative controls) did not give purplish red Fe III XO halos (right holes).

    Journal: PLoS ONE

    Article Title: A Highly Sensitive Method for Quantitative Determination of L-Amino Acid Oxidase Activity Based on the Visualization of Ferric-Xylenol Orange Formation

    doi: 10.1371/journal.pone.0082483

    Figure Lengend Snippet: Characterization of LAAO activities from Pseudoalteromonas sp. R3 (R3-LAAO) with L-Leu and L-Met as substrates, respectively (A) and from Crotalus adamanteus venom (caLAAO) with L-Leu as substrate (B) based on the Fe III XO agar assay. On the basis of the diameters of the formed purplish red Fe III XO halos, the concentrations of H 2 O 2 produced by LAAO activities were calculated with the equations in Figure 2 . The corresponding standard H 2 O 2 solutions as indicated above the corresponding holes were used to confirm the accuracy of Fe III XO agar assay. The diameters of the purplish red halos were marked below the holes. Without R3-LAAO, both L-Leu and L-Met (negative controls) did not give purplish red Fe III XO halos (right holes).

    Article Snippet: Stereospecific oxidation of amino acid by LAAO activity The stereospecific oxidation reaction was performed with 10 mM of each amino acid in 10 mL of R3-LAAO solution harvested from Psudoalteromonas sp. R3 culture supernatant or caLAAO solution (mixture of 1 µL caLAAO with 10 mL of 0.1 M PBS buffer with pH 7.5, Worthington Biochemical Corporation, USA).

    Techniques: Produced