ly294002  (Alomone Labs)


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    Structured Review

    Alomone Labs ly294002
    IMP3 exerts its proproliferative effects through the IGF-2-PI3K and IGF-2-MAPK cascades. A , viability was measured by MTT assay at indicated time points for H1299 vector and IMP3 stable clones treated with a neutralizing antibody for IGF-2 as indicated. The assays were carried out in triplicate, and the mean value for each cell type at each time point was used to generate the graph. A one-way analysis of variance was carried out to test the significance of the observed differences between the groups and a p ≤ 0.05 is represented with an asterisk , p ≤ 0.01 is represented as double asterisks and p ≤ 0.0001 is represented as triple asterisks. B , viability was measured by MTT assay as an indicator of cell proliferation at the indicated time points for either mock, cyclophilin ( cyclo. ), or IMP3 siRNA-transfected U138 cells. IGF-2 was exogenously supplemented as indicated to monitor its ability to rescue the effects of IMP3 knockdown. A one-way analysis of variance was carried out to test the significance of the observed differences between the groups. A p value ≤ 0.05 is represented as an asterisk , which is significant, and NS refers to nonsignificant difference. C , equal amounts of total protein lysates from cyclophilin and IMP3 siRNA-treated U138 cells were subjected to Western blotting with the indicated antibodies. Note that the intensity of phospho-mTOR, phospho-Akt, phospho-4EBP1, phospho-MEK1/2, and phospho-ERK1/2 bands reduces in lane 2 compared with lane 1 , whereas that of total the corresponding total proteins remain unchanged. D , viability was measured by MTT assay on day 9 after plating for the U373 vector clone (#6) and IMP3 stable clones (#5 and 17) treated with dimethyl sulfoxide (vehicle), <t>LY294002</t> (20 μ m ), or U0126 (10 μ m ). Please note that dimethyl sulfoxide ( DMSO )-treated IMP3 stable clone (#5 and #17) grow faster than vector stable clone (compare light gray bars ). Both LY294002 and U0126 treated IMP3 stable clone (#5 and #17) failed to show faster growth than vector stable clone (compare dark gray and black bars with light gray bars ).
    Ly294002, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 93/100, based on 32 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ly294002/product/Alomone Labs
    Average 93 stars, based on 32 article reviews
    Price from $9.99 to $1999.99
    ly294002 - by Bioz Stars, 2022-12
    93/100 stars

    Images

    1) Product Images from "Insulin Growth Factor-2 Binding Protein 3 (IGF2BP3) Is a Glioblastoma-specific Marker That Activates Phosphatidylinositol 3-Kinase/Mitogen-activated Protein Kinase (PI3K/MAPK) Pathways by Modulating IGF-2 *"

    Article Title: Insulin Growth Factor-2 Binding Protein 3 (IGF2BP3) Is a Glioblastoma-specific Marker That Activates Phosphatidylinositol 3-Kinase/Mitogen-activated Protein Kinase (PI3K/MAPK) Pathways by Modulating IGF-2 *

    Journal: The Journal of Biological Chemistry

    doi: 10.1074/jbc.M110.178012

    IMP3 exerts its proproliferative effects through the IGF-2-PI3K and IGF-2-MAPK cascades. A , viability was measured by MTT assay at indicated time points for H1299 vector and IMP3 stable clones treated with a neutralizing antibody for IGF-2 as indicated. The assays were carried out in triplicate, and the mean value for each cell type at each time point was used to generate the graph. A one-way analysis of variance was carried out to test the significance of the observed differences between the groups and a p ≤ 0.05 is represented with an asterisk , p ≤ 0.01 is represented as double asterisks and p ≤ 0.0001 is represented as triple asterisks. B , viability was measured by MTT assay as an indicator of cell proliferation at the indicated time points for either mock, cyclophilin ( cyclo. ), or IMP3 siRNA-transfected U138 cells. IGF-2 was exogenously supplemented as indicated to monitor its ability to rescue the effects of IMP3 knockdown. A one-way analysis of variance was carried out to test the significance of the observed differences between the groups. A p value ≤ 0.05 is represented as an asterisk , which is significant, and NS refers to nonsignificant difference. C , equal amounts of total protein lysates from cyclophilin and IMP3 siRNA-treated U138 cells were subjected to Western blotting with the indicated antibodies. Note that the intensity of phospho-mTOR, phospho-Akt, phospho-4EBP1, phospho-MEK1/2, and phospho-ERK1/2 bands reduces in lane 2 compared with lane 1 , whereas that of total the corresponding total proteins remain unchanged. D , viability was measured by MTT assay on day 9 after plating for the U373 vector clone (#6) and IMP3 stable clones (#5 and 17) treated with dimethyl sulfoxide (vehicle), LY294002 (20 μ m ), or U0126 (10 μ m ). Please note that dimethyl sulfoxide ( DMSO )-treated IMP3 stable clone (#5 and #17) grow faster than vector stable clone (compare light gray bars ). Both LY294002 and U0126 treated IMP3 stable clone (#5 and #17) failed to show faster growth than vector stable clone (compare dark gray and black bars with light gray bars ).
    Figure Legend Snippet: IMP3 exerts its proproliferative effects through the IGF-2-PI3K and IGF-2-MAPK cascades. A , viability was measured by MTT assay at indicated time points for H1299 vector and IMP3 stable clones treated with a neutralizing antibody for IGF-2 as indicated. The assays were carried out in triplicate, and the mean value for each cell type at each time point was used to generate the graph. A one-way analysis of variance was carried out to test the significance of the observed differences between the groups and a p ≤ 0.05 is represented with an asterisk , p ≤ 0.01 is represented as double asterisks and p ≤ 0.0001 is represented as triple asterisks. B , viability was measured by MTT assay as an indicator of cell proliferation at the indicated time points for either mock, cyclophilin ( cyclo. ), or IMP3 siRNA-transfected U138 cells. IGF-2 was exogenously supplemented as indicated to monitor its ability to rescue the effects of IMP3 knockdown. A one-way analysis of variance was carried out to test the significance of the observed differences between the groups. A p value ≤ 0.05 is represented as an asterisk , which is significant, and NS refers to nonsignificant difference. C , equal amounts of total protein lysates from cyclophilin and IMP3 siRNA-treated U138 cells were subjected to Western blotting with the indicated antibodies. Note that the intensity of phospho-mTOR, phospho-Akt, phospho-4EBP1, phospho-MEK1/2, and phospho-ERK1/2 bands reduces in lane 2 compared with lane 1 , whereas that of total the corresponding total proteins remain unchanged. D , viability was measured by MTT assay on day 9 after plating for the U373 vector clone (#6) and IMP3 stable clones (#5 and 17) treated with dimethyl sulfoxide (vehicle), LY294002 (20 μ m ), or U0126 (10 μ m ). Please note that dimethyl sulfoxide ( DMSO )-treated IMP3 stable clone (#5 and #17) grow faster than vector stable clone (compare light gray bars ). Both LY294002 and U0126 treated IMP3 stable clone (#5 and #17) failed to show faster growth than vector stable clone (compare dark gray and black bars with light gray bars ).

    Techniques Used: MTT Assay, Plasmid Preparation, Clone Assay, Transfection, Western Blot, Stable Transfection

    2) Product Images from "PI3K?-Dependent Signaling in Mouse Olfactory Receptor Neurons"

    Article Title: PI3K?-Dependent Signaling in Mouse Olfactory Receptor Neurons

    Journal: Chemical Senses

    doi: 10.1093/chemse/bjq020

    PI3Kγ KO mice show a large reduction in PI3K-mediated signaling upon odorant stimulation. (A) PI3Kγ KO mice show effects of odorant response enhancement to the pan-specific PI3K inhibitors wortmannin and LY294002 in a reduced number of
    Figure Legend Snippet: PI3Kγ KO mice show a large reduction in PI3K-mediated signaling upon odorant stimulation. (A) PI3Kγ KO mice show effects of odorant response enhancement to the pan-specific PI3K inhibitors wortmannin and LY294002 in a reduced number of

    Techniques Used: Mouse Assay

    Odorant-induced PI3K-dependent signaling in mouse ORNs. (A) ORNs from wt mice show enhanced responses to complex odorant stimulation following inhibition of PI3K by wortmannin and LY294002. Cells for analysis were selected by responsiveness to H100 in
    Figure Legend Snippet: Odorant-induced PI3K-dependent signaling in mouse ORNs. (A) ORNs from wt mice show enhanced responses to complex odorant stimulation following inhibition of PI3K by wortmannin and LY294002. Cells for analysis were selected by responsiveness to H100 in

    Techniques Used: Mouse Assay, Inhibition

    3) Product Images from "PI3K?-Dependent Signaling in Mouse Olfactory Receptor Neurons"

    Article Title: PI3K?-Dependent Signaling in Mouse Olfactory Receptor Neurons

    Journal: Chemical Senses

    doi: 10.1093/chemse/bjq020

    PI3Kγ KO mice show a large reduction in PI3K-mediated signaling upon odorant stimulation. (A) PI3Kγ KO mice show effects of odorant response enhancement to the pan-specific PI3K inhibitors wortmannin and LY294002 in a reduced number of
    Figure Legend Snippet: PI3Kγ KO mice show a large reduction in PI3K-mediated signaling upon odorant stimulation. (A) PI3Kγ KO mice show effects of odorant response enhancement to the pan-specific PI3K inhibitors wortmannin and LY294002 in a reduced number of

    Techniques Used: Mouse Assay

    Odorant-induced PI3K-dependent signaling in mouse ORNs. (A) ORNs from wt mice show enhanced responses to complex odorant stimulation following inhibition of PI3K by wortmannin and LY294002. Cells for analysis were selected by responsiveness to H100 in
    Figure Legend Snippet: Odorant-induced PI3K-dependent signaling in mouse ORNs. (A) ORNs from wt mice show enhanced responses to complex odorant stimulation following inhibition of PI3K by wortmannin and LY294002. Cells for analysis were selected by responsiveness to H100 in

    Techniques Used: Mouse Assay, Inhibition

    4) Product Images from "PI3K?-Dependent Signaling in Mouse Olfactory Receptor Neurons"

    Article Title: PI3K?-Dependent Signaling in Mouse Olfactory Receptor Neurons

    Journal: Chemical Senses

    doi: 10.1093/chemse/bjq020

    PI3Kγ KO mice show a large reduction in PI3K-mediated signaling upon odorant stimulation. (A) PI3Kγ KO mice show effects of odorant response enhancement to the pan-specific PI3K inhibitors wortmannin and LY294002 in a reduced number of
    Figure Legend Snippet: PI3Kγ KO mice show a large reduction in PI3K-mediated signaling upon odorant stimulation. (A) PI3Kγ KO mice show effects of odorant response enhancement to the pan-specific PI3K inhibitors wortmannin and LY294002 in a reduced number of

    Techniques Used: Mouse Assay

    Odorant-induced PI3K-dependent signaling in mouse ORNs. (A) ORNs from wt mice show enhanced responses to complex odorant stimulation following inhibition of PI3K by wortmannin and LY294002. Cells for analysis were selected by responsiveness to H100 in
    Figure Legend Snippet: Odorant-induced PI3K-dependent signaling in mouse ORNs. (A) ORNs from wt mice show enhanced responses to complex odorant stimulation following inhibition of PI3K by wortmannin and LY294002. Cells for analysis were selected by responsiveness to H100 in

    Techniques Used: Mouse Assay, Inhibition

    5) Product Images from "Fatty acid synthase as a novel target for meningioma therapy"

    Article Title: Fatty acid synthase as a novel target for meningioma therapy

    Journal: Neuro-Oncology

    doi: 10.1093/neuonc/noq004

    FAS expression can be induced in serum-starved IOMM-Lee cells by insulin stimulation (A). Inhibition of PI3K signaling by wortmannin or Ly294002 effectively downregulates the FAS levels in serum-starved IOMM-Lee cells stimulated with insulin, while inhibition
    Figure Legend Snippet: FAS expression can be induced in serum-starved IOMM-Lee cells by insulin stimulation (A). Inhibition of PI3K signaling by wortmannin or Ly294002 effectively downregulates the FAS levels in serum-starved IOMM-Lee cells stimulated with insulin, while inhibition

    Techniques Used: Expressing, Inhibition

    6) Product Images from "Fatty acid synthase as a novel target for meningioma therapy"

    Article Title: Fatty acid synthase as a novel target for meningioma therapy

    Journal: Neuro-Oncology

    doi: 10.1093/neuonc/noq004

    FAS expression can be induced in serum-starved IOMM-Lee cells by insulin stimulation (A). Inhibition of PI3K signaling by wortmannin or Ly294002 effectively downregulates the FAS levels in serum-starved IOMM-Lee cells stimulated with insulin, while inhibition
    Figure Legend Snippet: FAS expression can be induced in serum-starved IOMM-Lee cells by insulin stimulation (A). Inhibition of PI3K signaling by wortmannin or Ly294002 effectively downregulates the FAS levels in serum-starved IOMM-Lee cells stimulated with insulin, while inhibition

    Techniques Used: Expressing, Inhibition

    7) Product Images from "Hypoxia Up-regulates CD36 Expression and Function via Hypoxia-inducible Factor-1- and Phosphatidylinositol 3-Kinase-dependent Mechanisms *"

    Article Title: Hypoxia Up-regulates CD36 Expression and Function via Hypoxia-inducible Factor-1- and Phosphatidylinositol 3-Kinase-dependent Mechanisms *

    Journal: The Journal of Biological Chemistry

    doi: 10.1074/jbc.M109.033480

    Implication of the PI3K pathway in the hypoxic accumulation of CD36. ARPEs were incubated with or without PI3K ( A ) (wortmannin, 200 n m ; LY294002, 20 μ m ) and mTOR (rapamycin, 100 n m ) inhibitors, or PI3K p85α siRNA ( B and C ) (30 n m ) prior
    Figure Legend Snippet: Implication of the PI3K pathway in the hypoxic accumulation of CD36. ARPEs were incubated with or without PI3K ( A ) (wortmannin, 200 n m ; LY294002, 20 μ m ) and mTOR (rapamycin, 100 n m ) inhibitors, or PI3K p85α siRNA ( B and C ) (30 n m ) prior

    Techniques Used: Incubation

    8) Product Images from "Hypoxia Up-regulates CD36 Expression and Function via Hypoxia-inducible Factor-1- and Phosphatidylinositol 3-Kinase-dependent Mechanisms *"

    Article Title: Hypoxia Up-regulates CD36 Expression and Function via Hypoxia-inducible Factor-1- and Phosphatidylinositol 3-Kinase-dependent Mechanisms *

    Journal: The Journal of Biological Chemistry

    doi: 10.1074/jbc.M109.033480

    Implication of the PI3K pathway in the hypoxic accumulation of CD36. ARPEs were incubated with or without PI3K ( A ) (wortmannin, 200 n m ; LY294002, 20 μ m ) and mTOR (rapamycin, 100 n m ) inhibitors, or PI3K p85α siRNA ( B and C ) (30 n m ) prior
    Figure Legend Snippet: Implication of the PI3K pathway in the hypoxic accumulation of CD36. ARPEs were incubated with or without PI3K ( A ) (wortmannin, 200 n m ; LY294002, 20 μ m ) and mTOR (rapamycin, 100 n m ) inhibitors, or PI3K p85α siRNA ( B and C ) (30 n m ) prior

    Techniques Used: Incubation

    9) Product Images from "Hypoxia Up-regulates CD36 Expression and Function via Hypoxia-inducible Factor-1- and Phosphatidylinositol 3-Kinase-dependent Mechanisms *"

    Article Title: Hypoxia Up-regulates CD36 Expression and Function via Hypoxia-inducible Factor-1- and Phosphatidylinositol 3-Kinase-dependent Mechanisms *

    Journal: The Journal of Biological Chemistry

    doi: 10.1074/jbc.M109.033480

    Implication of the PI3K pathway in the hypoxic accumulation of CD36. ARPEs were incubated with or without PI3K ( A ) (wortmannin, 200 n m ; LY294002, 20 μ m ) and mTOR (rapamycin, 100 n m ) inhibitors, or PI3K p85α siRNA ( B and C ) (30 n m ) prior
    Figure Legend Snippet: Implication of the PI3K pathway in the hypoxic accumulation of CD36. ARPEs were incubated with or without PI3K ( A ) (wortmannin, 200 n m ; LY294002, 20 μ m ) and mTOR (rapamycin, 100 n m ) inhibitors, or PI3K p85α siRNA ( B and C ) (30 n m ) prior

    Techniques Used: Incubation

    10) Product Images from "Hypoxia Up-regulates CD36 Expression and Function via Hypoxia-inducible Factor-1- and Phosphatidylinositol 3-Kinase-dependent Mechanisms *"

    Article Title: Hypoxia Up-regulates CD36 Expression and Function via Hypoxia-inducible Factor-1- and Phosphatidylinositol 3-Kinase-dependent Mechanisms *

    Journal: The Journal of Biological Chemistry

    doi: 10.1074/jbc.M109.033480

    Implication of the PI3K pathway in the hypoxic accumulation of CD36. ARPEs were incubated with or without PI3K ( A ) (wortmannin, 200 n m ; LY294002, 20 μ m ) and mTOR (rapamycin, 100 n m ) inhibitors, or PI3K p85α siRNA ( B and C ) (30 n m ) prior
    Figure Legend Snippet: Implication of the PI3K pathway in the hypoxic accumulation of CD36. ARPEs were incubated with or without PI3K ( A ) (wortmannin, 200 n m ; LY294002, 20 μ m ) and mTOR (rapamycin, 100 n m ) inhibitors, or PI3K p85α siRNA ( B and C ) (30 n m ) prior

    Techniques Used: Incubation

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  • 93
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