lif  (Alomone Labs)


Bioz Verified Symbol Alomone Labs is a verified supplier
Bioz Manufacturer Symbol Alomone Labs manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 88

    Structured Review

    Alomone Labs lif
    Lif, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 88/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/lif/product/Alomone Labs
    Average 88 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    lif - by Bioz Stars, 2022-05
    88/100 stars

    Images

    Similar Products

  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 93
    Alomone Labs channel kir subunits kir2 1
    Inward rectifier K + current I K1 expression and function in transgenic atrial myocytes. Experimental animals involved wild‐type mice (WT) and transgenic mice (TG) expressing the repressor isoform of cyclic adenosine monophosphate response element modulator, CREM‐IbΔC‐X, and were distributed according to age in 6‐week‐old (WT 6w and TG 6w ) and 12‐week‐old (WT 12w and TG 12w ) groups. A , Representative traces of inward rectifiers K + channel (I K1 ) recorded in WT 12w and TG 12w myocytes before and after application of 10 μmol/L BaCl 2 using a ramp protocol (inset, scale bar 0.2 seconds). The traces show current densitiy (expressed in picoamperes/picofarads [pA/pF]) over time. ( Aa ). Current–voltage plots of I K1 averaged from traces of all cells. For WT 6w , n/N are 8/5, for TG 6w n/N are 10/6, for WT 12w n/N are 13/7, and for TG 12w n/N are 9/6 ( Ab ). B , Data show mean±SE of relative mRNA levels of atrial K + voltage‐gated channel subfamily J member 2 ( Kcnj2 ) and 4 ( Kcnj4 ) normalized to WT 6w vs Hprt1 (hypoxanthine phosphoribosyltransferase 1) as the housekeeping gene; N=8 per group. Y‐axes scale is Log2. Ca through c , Representative immunoblots ( Ca ) and quantification of K + inwardly rectifying channel subunits <t>Kir2.1</t> ( Cb ) and Kir2.3 ( Cc ) protein levels normalized to calsequestrin (CSQ). Data show mean±SE of 7 mice per group. Da through b , Representative action potentials recorded before (normal Tyrode [NT] as control) and after application of 10 μmol/L BaCl 2 in 1 atrial myocyte of each group ( Da ). Quantification of the percentages of action potential duration (APD) change in BaCl 2 vs NT at 50% (APD 50 ) and 90% (APD 90 ) repolarization. Data show mean±SE of n/N for WT 6w (7/5), TG 6w (5/5), WT 12w (11/6), and TG 12w (5/4) ( Db ). * P
    Channel Kir Subunits Kir2 1, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/channel kir subunits kir2 1/product/Alomone Labs
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    channel kir subunits kir2 1 - by Bioz Stars, 2022-05
    93/100 stars
      Buy from Supplier

    95
    Alomone Labs rabbit anti cav1 2 antibody
    Treatment effects of metformin on the mRNA and protein levels of <t>Cav1.2</t> in diabetic mice. (A) Relative levels of CACNA1C with metformin in diabetic mice detected by real-time PCR, n=6. (B) Relative levels of Cav1.2 in the Ctrl group, DM group, and the metformin-treatment group detected by western blot, n=6. (C) Representative confocal images of α-Actinin (left) or CaV1.2 (middle) in the Ctrl group, DM group, and the metformin-treatment group. (D) Relative levels of Cav1.2 in the Ctrl group, DM group, and the metformin-treatment group by immunofluorescences, n=9. Values represent mean ± SEM, *P
    Rabbit Anti Cav1 2 Antibody, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rabbit anti cav1 2 antibody/product/Alomone Labs
    Average 95 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    rabbit anti cav1 2 antibody - by Bioz Stars, 2022-05
    95/100 stars
      Buy from Supplier

    93
    Alomone Labs anti cav1 3
    Deletion of RBPs reduces Ca 2+ currents in presynaptic rod bipolar cells forming ribbon synapses on postsynaptic AII amacrine cells. ( A ) Selective loss of presynaptic L-type Ca 2+ channels from ribbon synapses formed by rod bipolar cells. Representative confocal images of RBP WT ( Left ) and RBP DKO ( Right ) retina sections stained for PKCα (green, to identify rod bipolar neurons), VGlut1 (purple, to identify presynaptic terminals), and the L-type Ca 2+ -channel <t>CaV1.3</t> (red). ( B ) Summary graphs of the intensity of PKCα fluorescent signals in WT(gray) and DKO (blue) rod bipolar cell terminals ( Left ), and terminal size (area, Right ) defined by PKC staining. Number of experiments (images/mice): RBP WT, 79/3; RBP DKO, 70/3. ( C ) Relative vGluT1 ( Left ) and CaV1.3 ( Right ) staining intensity normalized by PKCα signals. Number of experiments (images/mice): RBP WT, 79/3; RBP DKO, 70/3. ( D ) Experimental configuration for Ca 2+ -current recordings ( Left ) and representative experiment ( Right Top ) schematic of depolarization protocol; ( Right Bottom ) Sample traces for an RBP control (black) and an RBP DKO (blue) bipolar cell. ( E ) Deletion of RBP1,2 severely impairs presynaptic Ca 2+ -current density. Summary plot of the Ca 2+ -current charge transfer over 50 ms as a function of the membrane voltage in RBP1,2 DKO (blue) rod bipolar cells and corresponding littermate controls (gray). ( F and G ) Incremental contribution of RBP1 ( F ) and RBP2 ( G ) to the presynaptic Ca 2+ -channel density of ribbon synapses. Same as E , but for littermate control ( F and G , gray) and RBP1 KO ( F , orange) or RBP2 KO ( G , green) mice. ( H ) Summary graphs of the Ca 2+ -current charge transfer induced by a 50-ms depolarization to −20 mV in RBP1,2 DKO, RBP1 KO, or RBP2 KO mice, normalized to the controls analyzed in the same experiments. Number of experiments as in E – G . ( I ) Summary graphs of whole-cell capacitance ( Left ) and input resistance ( Right ) in the same rod bipolar cells used to measure whole-cell presynaptic Ca 2+ currents. Number of experiments as in E . All summary graphs are mean ± SD. Statistical analyses were performed by either Student's t test ( B , C , H , and I ) or by ANOVA followed by a Bonferroni post hoc test ( E – G ), comparing RBP DKO with RBP WT (* P
    Anti Cav1 3, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti cav1 3/product/Alomone Labs
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    anti cav1 3 - by Bioz Stars, 2022-05
    93/100 stars
      Buy from Supplier

    88
    Alomone Labs recombinant human leukemia inhibitory factor lif
    Notch activation is increased by <t>LIF</t> and CNTF. Panel A depicts Western blot analysis of Notch1 and Notch1 intracellular domain (ICD) from neurospheres treated with <t>5ng/mL</t> of LIF or CNTF for 24 hours. Thirty micrograms of total protein from 2 representative
    Recombinant Human Leukemia Inhibitory Factor Lif, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 88/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/recombinant human leukemia inhibitory factor lif/product/Alomone Labs
    Average 88 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    recombinant human leukemia inhibitory factor lif - by Bioz Stars, 2022-05
    88/100 stars
      Buy from Supplier

    Image Search Results


    Inward rectifier K + current I K1 expression and function in transgenic atrial myocytes. Experimental animals involved wild‐type mice (WT) and transgenic mice (TG) expressing the repressor isoform of cyclic adenosine monophosphate response element modulator, CREM‐IbΔC‐X, and were distributed according to age in 6‐week‐old (WT 6w and TG 6w ) and 12‐week‐old (WT 12w and TG 12w ) groups. A , Representative traces of inward rectifiers K + channel (I K1 ) recorded in WT 12w and TG 12w myocytes before and after application of 10 μmol/L BaCl 2 using a ramp protocol (inset, scale bar 0.2 seconds). The traces show current densitiy (expressed in picoamperes/picofarads [pA/pF]) over time. ( Aa ). Current–voltage plots of I K1 averaged from traces of all cells. For WT 6w , n/N are 8/5, for TG 6w n/N are 10/6, for WT 12w n/N are 13/7, and for TG 12w n/N are 9/6 ( Ab ). B , Data show mean±SE of relative mRNA levels of atrial K + voltage‐gated channel subfamily J member 2 ( Kcnj2 ) and 4 ( Kcnj4 ) normalized to WT 6w vs Hprt1 (hypoxanthine phosphoribosyltransferase 1) as the housekeeping gene; N=8 per group. Y‐axes scale is Log2. Ca through c , Representative immunoblots ( Ca ) and quantification of K + inwardly rectifying channel subunits Kir2.1 ( Cb ) and Kir2.3 ( Cc ) protein levels normalized to calsequestrin (CSQ). Data show mean±SE of 7 mice per group. Da through b , Representative action potentials recorded before (normal Tyrode [NT] as control) and after application of 10 μmol/L BaCl 2 in 1 atrial myocyte of each group ( Da ). Quantification of the percentages of action potential duration (APD) change in BaCl 2 vs NT at 50% (APD 50 ) and 90% (APD 90 ) repolarization. Data show mean±SE of n/N for WT 6w (7/5), TG 6w (5/5), WT 12w (11/6), and TG 12w (5/4) ( Db ). * P

    Journal: Journal of the American Heart Association: Cardiovascular and Cerebrovascular Disease

    Article Title: Inward Rectifier K+ Currents Contribute to the Proarrhythmic Electrical Phenotype of Atria Overexpressing Cyclic Adenosine Monophosphate Response Element Modulator Isoform CREM‐IbΔC‐X

    doi: 10.1161/JAHA.119.016144

    Figure Lengend Snippet: Inward rectifier K + current I K1 expression and function in transgenic atrial myocytes. Experimental animals involved wild‐type mice (WT) and transgenic mice (TG) expressing the repressor isoform of cyclic adenosine monophosphate response element modulator, CREM‐IbΔC‐X, and were distributed according to age in 6‐week‐old (WT 6w and TG 6w ) and 12‐week‐old (WT 12w and TG 12w ) groups. A , Representative traces of inward rectifiers K + channel (I K1 ) recorded in WT 12w and TG 12w myocytes before and after application of 10 μmol/L BaCl 2 using a ramp protocol (inset, scale bar 0.2 seconds). The traces show current densitiy (expressed in picoamperes/picofarads [pA/pF]) over time. ( Aa ). Current–voltage plots of I K1 averaged from traces of all cells. For WT 6w , n/N are 8/5, for TG 6w n/N are 10/6, for WT 12w n/N are 13/7, and for TG 12w n/N are 9/6 ( Ab ). B , Data show mean±SE of relative mRNA levels of atrial K + voltage‐gated channel subfamily J member 2 ( Kcnj2 ) and 4 ( Kcnj4 ) normalized to WT 6w vs Hprt1 (hypoxanthine phosphoribosyltransferase 1) as the housekeeping gene; N=8 per group. Y‐axes scale is Log2. Ca through c , Representative immunoblots ( Ca ) and quantification of K + inwardly rectifying channel subunits Kir2.1 ( Cb ) and Kir2.3 ( Cc ) protein levels normalized to calsequestrin (CSQ). Data show mean±SE of 7 mice per group. Da through b , Representative action potentials recorded before (normal Tyrode [NT] as control) and after application of 10 μmol/L BaCl 2 in 1 atrial myocyte of each group ( Da ). Quantification of the percentages of action potential duration (APD) change in BaCl 2 vs NT at 50% (APD 50 ) and 90% (APD 90 ) repolarization. Data show mean±SE of n/N for WT 6w (7/5), TG 6w (5/5), WT 12w (11/6), and TG 12w (5/4) ( Db ). * P

    Article Snippet: The following rabbit polyclonal primary antibodies were used against K + voltage‐gated channels (Kv) subunits Kv4.2 (1:200, APC 023, Alomone Labs, Jerusalem, Israel), Kv4.3 (1:200, APC 017, Alomone Labs), K + channel interacting protein 2 (KChIP2) (1:200, sc‐25685, Santa Cruz Biotechnology Inc, Santa Cruz, CA), and K + inwardly rectifying channel (Kir) subunits Kir2.1 (1:200, APC 159, Alomone Labs), Kir2.3 (1:1000, APC 032, Alomone Labs), Kir3.1 (1:200, APC 005, Alomone Labs), Kir3.4 (1:200, APC 027, Alomone Labs), and as loading control calsequestrin (1:2500, PA1‐913, Thermo Fisher Scientific).

    Techniques: Expressing, Transgenic Assay, Mouse Assay, Western Blot

    Treatment effects of metformin on the mRNA and protein levels of Cav1.2 in diabetic mice. (A) Relative levels of CACNA1C with metformin in diabetic mice detected by real-time PCR, n=6. (B) Relative levels of Cav1.2 in the Ctrl group, DM group, and the metformin-treatment group detected by western blot, n=6. (C) Representative confocal images of α-Actinin (left) or CaV1.2 (middle) in the Ctrl group, DM group, and the metformin-treatment group. (D) Relative levels of Cav1.2 in the Ctrl group, DM group, and the metformin-treatment group by immunofluorescences, n=9. Values represent mean ± SEM, *P

    Journal: Frontiers in Pharmacology

    Article Title: Metformin Shortens Prolonged QT Interval in Diabetic Mice by Inhibiting L-Type Calcium Current: A Possible Therapeutic Approach

    doi: 10.3389/fphar.2020.00614

    Figure Lengend Snippet: Treatment effects of metformin on the mRNA and protein levels of Cav1.2 in diabetic mice. (A) Relative levels of CACNA1C with metformin in diabetic mice detected by real-time PCR, n=6. (B) Relative levels of Cav1.2 in the Ctrl group, DM group, and the metformin-treatment group detected by western blot, n=6. (C) Representative confocal images of α-Actinin (left) or CaV1.2 (middle) in the Ctrl group, DM group, and the metformin-treatment group. (D) Relative levels of Cav1.2 in the Ctrl group, DM group, and the metformin-treatment group by immunofluorescences, n=9. Values represent mean ± SEM, *P

    Article Snippet: Subsequently, the cells were treated with a penetrant (0.6% TritonX-100 and bovine serum albumin) for 1 hour, covered with blocking medium (Goat serum, Boster) for 2 hours, and incubated with the following primary antibodies overnight at 4°C: rabbit anti-Cav1.2 antibody (1:200; Cat. No. ACC-003; Alomone Labs, Jerusalem, Israel) and GAPDH antibody (1:1000; Cat. No. Ab181602; Abcam).

    Techniques: Mouse Assay, Real-time Polymerase Chain Reaction, Western Blot

    Treatment effects of metformin on the mRNA and protein levels of Cav1.2 in primary cultured neonatal mice cardiomyocytes. (A) Relative levels of CACNA1C with metformin of primary cardiomyocytes in NC group, HG group and metformin-treatment group detected by real-time PCR, n=6. (B) Relative levels of Cav1.2 of primary cardiomyocytes in NC group, HG group and metformin-treatment group detected by western blot, n=6. (C) Representative confocal images of α-Actinin (left) or CaV1.2 (middle) of primary cardiomyocytes in NC group, HG group and metformin-treatment group. (D) Relative levels of Cav1.2 of primary cardiomyocytes in NC group, HG group and metformin-treatment group detected by immunofluorescences, n=9. Values represent mean ± SEM, *P

    Journal: Frontiers in Pharmacology

    Article Title: Metformin Shortens Prolonged QT Interval in Diabetic Mice by Inhibiting L-Type Calcium Current: A Possible Therapeutic Approach

    doi: 10.3389/fphar.2020.00614

    Figure Lengend Snippet: Treatment effects of metformin on the mRNA and protein levels of Cav1.2 in primary cultured neonatal mice cardiomyocytes. (A) Relative levels of CACNA1C with metformin of primary cardiomyocytes in NC group, HG group and metformin-treatment group detected by real-time PCR, n=6. (B) Relative levels of Cav1.2 of primary cardiomyocytes in NC group, HG group and metformin-treatment group detected by western blot, n=6. (C) Representative confocal images of α-Actinin (left) or CaV1.2 (middle) of primary cardiomyocytes in NC group, HG group and metformin-treatment group. (D) Relative levels of Cav1.2 of primary cardiomyocytes in NC group, HG group and metformin-treatment group detected by immunofluorescences, n=9. Values represent mean ± SEM, *P

    Article Snippet: Subsequently, the cells were treated with a penetrant (0.6% TritonX-100 and bovine serum albumin) for 1 hour, covered with blocking medium (Goat serum, Boster) for 2 hours, and incubated with the following primary antibodies overnight at 4°C: rabbit anti-Cav1.2 antibody (1:200; Cat. No. ACC-003; Alomone Labs, Jerusalem, Israel) and GAPDH antibody (1:1000; Cat. No. Ab181602; Abcam).

    Techniques: Cell Culture, Mouse Assay, Real-time Polymerase Chain Reaction, Western Blot

    Deletion of RBPs reduces Ca 2+ currents in presynaptic rod bipolar cells forming ribbon synapses on postsynaptic AII amacrine cells. ( A ) Selective loss of presynaptic L-type Ca 2+ channels from ribbon synapses formed by rod bipolar cells. Representative confocal images of RBP WT ( Left ) and RBP DKO ( Right ) retina sections stained for PKCα (green, to identify rod bipolar neurons), VGlut1 (purple, to identify presynaptic terminals), and the L-type Ca 2+ -channel CaV1.3 (red). ( B ) Summary graphs of the intensity of PKCα fluorescent signals in WT(gray) and DKO (blue) rod bipolar cell terminals ( Left ), and terminal size (area, Right ) defined by PKC staining. Number of experiments (images/mice): RBP WT, 79/3; RBP DKO, 70/3. ( C ) Relative vGluT1 ( Left ) and CaV1.3 ( Right ) staining intensity normalized by PKCα signals. Number of experiments (images/mice): RBP WT, 79/3; RBP DKO, 70/3. ( D ) Experimental configuration for Ca 2+ -current recordings ( Left ) and representative experiment ( Right Top ) schematic of depolarization protocol; ( Right Bottom ) Sample traces for an RBP control (black) and an RBP DKO (blue) bipolar cell. ( E ) Deletion of RBP1,2 severely impairs presynaptic Ca 2+ -current density. Summary plot of the Ca 2+ -current charge transfer over 50 ms as a function of the membrane voltage in RBP1,2 DKO (blue) rod bipolar cells and corresponding littermate controls (gray). ( F and G ) Incremental contribution of RBP1 ( F ) and RBP2 ( G ) to the presynaptic Ca 2+ -channel density of ribbon synapses. Same as E , but for littermate control ( F and G , gray) and RBP1 KO ( F , orange) or RBP2 KO ( G , green) mice. ( H ) Summary graphs of the Ca 2+ -current charge transfer induced by a 50-ms depolarization to −20 mV in RBP1,2 DKO, RBP1 KO, or RBP2 KO mice, normalized to the controls analyzed in the same experiments. Number of experiments as in E – G . ( I ) Summary graphs of whole-cell capacitance ( Left ) and input resistance ( Right ) in the same rod bipolar cells used to measure whole-cell presynaptic Ca 2+ currents. Number of experiments as in E . All summary graphs are mean ± SD. Statistical analyses were performed by either Student's t test ( B , C , H , and I ) or by ANOVA followed by a Bonferroni post hoc test ( E – G ), comparing RBP DKO with RBP WT (* P

    Journal: Proceedings of the National Academy of Sciences of the United States of America

    Article Title: Efficient stimulus-secretion coupling at ribbon synapses requires RIM-binding protein tethering of L-type Ca2+ channels

    doi: 10.1073/pnas.1702991114

    Figure Lengend Snippet: Deletion of RBPs reduces Ca 2+ currents in presynaptic rod bipolar cells forming ribbon synapses on postsynaptic AII amacrine cells. ( A ) Selective loss of presynaptic L-type Ca 2+ channels from ribbon synapses formed by rod bipolar cells. Representative confocal images of RBP WT ( Left ) and RBP DKO ( Right ) retina sections stained for PKCα (green, to identify rod bipolar neurons), VGlut1 (purple, to identify presynaptic terminals), and the L-type Ca 2+ -channel CaV1.3 (red). ( B ) Summary graphs of the intensity of PKCα fluorescent signals in WT(gray) and DKO (blue) rod bipolar cell terminals ( Left ), and terminal size (area, Right ) defined by PKC staining. Number of experiments (images/mice): RBP WT, 79/3; RBP DKO, 70/3. ( C ) Relative vGluT1 ( Left ) and CaV1.3 ( Right ) staining intensity normalized by PKCα signals. Number of experiments (images/mice): RBP WT, 79/3; RBP DKO, 70/3. ( D ) Experimental configuration for Ca 2+ -current recordings ( Left ) and representative experiment ( Right Top ) schematic of depolarization protocol; ( Right Bottom ) Sample traces for an RBP control (black) and an RBP DKO (blue) bipolar cell. ( E ) Deletion of RBP1,2 severely impairs presynaptic Ca 2+ -current density. Summary plot of the Ca 2+ -current charge transfer over 50 ms as a function of the membrane voltage in RBP1,2 DKO (blue) rod bipolar cells and corresponding littermate controls (gray). ( F and G ) Incremental contribution of RBP1 ( F ) and RBP2 ( G ) to the presynaptic Ca 2+ -channel density of ribbon synapses. Same as E , but for littermate control ( F and G , gray) and RBP1 KO ( F , orange) or RBP2 KO ( G , green) mice. ( H ) Summary graphs of the Ca 2+ -current charge transfer induced by a 50-ms depolarization to −20 mV in RBP1,2 DKO, RBP1 KO, or RBP2 KO mice, normalized to the controls analyzed in the same experiments. Number of experiments as in E – G . ( I ) Summary graphs of whole-cell capacitance ( Left ) and input resistance ( Right ) in the same rod bipolar cells used to measure whole-cell presynaptic Ca 2+ currents. Number of experiments as in E . All summary graphs are mean ± SD. Statistical analyses were performed by either Student's t test ( B , C , H , and I ) or by ANOVA followed by a Bonferroni post hoc test ( E – G ), comparing RBP DKO with RBP WT (* P

    Article Snippet: Retina sections were then permeabilized with 0.4% Triton X-100, blocked with 3% goat serum, and subjected to conventional immunohistochemistry protocols using the following antibodies (overnight incubation at 4 °C): anti-PKCa (Sc-208, 1:200; Santa Cruz Biotechnology) to label bipolar cells, anti-VGluT1 to label bipolar cells terminals (Ab5905, 1:1,000; Millipore), and anti-Cav1.3 (ACC-005, 1:200; Alomone Labs) to label L-type Ca2+ channels.

    Techniques: Staining, Mouse Assay, Mass Spectrometry

    Notch activation is increased by LIF and CNTF. Panel A depicts Western blot analysis of Notch1 and Notch1 intracellular domain (ICD) from neurospheres treated with 5ng/mL of LIF or CNTF for 24 hours. Thirty micrograms of total protein from 2 representative

    Journal:

    Article Title: Leukemia inhibitory factor participates in the expansion of neural stem/progenitors after perinatal hypoxia/ischemia

    doi: 10.1016/J.NEUROSCIENCE.2007.06.015

    Figure Lengend Snippet: Notch activation is increased by LIF and CNTF. Panel A depicts Western blot analysis of Notch1 and Notch1 intracellular domain (ICD) from neurospheres treated with 5ng/mL of LIF or CNTF for 24 hours. Thirty micrograms of total protein from 2 representative

    Article Snippet: Neurosphere TreatmentSecondary neurospheres were cultured in either Pro-N media supplemented with 20 ng/mL EGF and 10 ng/ml FGF-2 or Pro-N media supplemented with 20 ng/mL EGF and 10 ng/ml FGF-2 and 5ng/mL of recombinant human Leukemia Inhibitory Factor (LIF) (Alomone Labs, Israel) for 24 hours or 6 days in vitro.

    Techniques: Activation Assay, Western Blot

    LIF enhances neural stem cell expansion/maintenance. For panels A and B, primary neurospheres were dissociated and replated into either control meduim or medium supplemented with 5ng/mL of LIF. The number and size of spheres was quantified at 6 days in

    Journal:

    Article Title: Leukemia inhibitory factor participates in the expansion of neural stem/progenitors after perinatal hypoxia/ischemia

    doi: 10.1016/J.NEUROSCIENCE.2007.06.015

    Figure Lengend Snippet: LIF enhances neural stem cell expansion/maintenance. For panels A and B, primary neurospheres were dissociated and replated into either control meduim or medium supplemented with 5ng/mL of LIF. The number and size of spheres was quantified at 6 days in

    Article Snippet: Neurosphere TreatmentSecondary neurospheres were cultured in either Pro-N media supplemented with 20 ng/mL EGF and 10 ng/ml FGF-2 or Pro-N media supplemented with 20 ng/mL EGF and 10 ng/ml FGF-2 and 5ng/mL of recombinant human Leukemia Inhibitory Factor (LIF) (Alomone Labs, Israel) for 24 hours or 6 days in vitro.

    Techniques:

    LIF induces both Notch1 and Delta-like-1 expression. Neurospheres were treated with 5ng of LIF or CNTF for 24h and the levels of Notch and Delta-like-1 mRNA were quantified by real time PCR. There were significant increases in mRNA expression for both

    Journal:

    Article Title: Leukemia inhibitory factor participates in the expansion of neural stem/progenitors after perinatal hypoxia/ischemia

    doi: 10.1016/J.NEUROSCIENCE.2007.06.015

    Figure Lengend Snippet: LIF induces both Notch1 and Delta-like-1 expression. Neurospheres were treated with 5ng of LIF or CNTF for 24h and the levels of Notch and Delta-like-1 mRNA were quantified by real time PCR. There were significant increases in mRNA expression for both

    Article Snippet: Neurosphere TreatmentSecondary neurospheres were cultured in either Pro-N media supplemented with 20 ng/mL EGF and 10 ng/ml FGF-2 or Pro-N media supplemented with 20 ng/mL EGF and 10 ng/ml FGF-2 and 5ng/mL of recombinant human Leukemia Inhibitory Factor (LIF) (Alomone Labs, Israel) for 24 hours or 6 days in vitro.

    Techniques: Expressing, Real-time Polymerase Chain Reaction