kt5720  (Alomone Labs)

Journal: The Journal of Neuroscience

Article Title: Phase-Dependent Molecular Requirements for Memory Reconsolidation: Differential Roles for Protein Synthesis and Protein Kinase A Activity

doi: 10.1523/JNEUROSCI.0890-06.2006

Figure Lengend Snippet: PKA activity is required after memory retrieval at 6 but not 24 h after training for later memory expression. Six hours ( A ) or 24 h ( B ) after a single CS/US pairing (0 h), animals received a CS stimulation or no stimulation (no CS), followed 10 min later by an injection with either a PKA inhibitor (KT5720) or vehicle. In each group, baseline levels are defined by the mean feeding response level to the CS (line) ± SEM (gray band) of a group of animals handled in parallel ( n = 17). A , ANOVAs followed by pairwise post hoc tests reveal that the two groups ( n = 24 and 21) first tested at 6 h after training and 10 min before injection show significantly (*) elevated feeding responses to the CS compared with baseline level. At the second test, 24 h after training, one-way ANOVAs detected a source of significant difference. Of the four CS/US paired groups, only the group that received the CS at 6 h after training followed by KT5720 injection shows a feeding response to the CS that does not differ from baseline. A two-way ANOVA comparing the feeding responses to the CS of the four CS/US paired groups at 24 h detected a significant interaction of memory retrieval at 6 h and subsequent PKA inhibition. B , The two groups ( n = 17 and 20) first tested at 24 h after training and 10 min before injection show significantly (*) elevated feeding responses to the CS compared with the baseline level. At 42 h after training, one-way ANOVAs and multiple post hoc ).

Article Snippet: The amnestic agent used was KT5720 (Alomone Labs, Jerusalem, Israel), a specific PKA inhibitor, at 10 μ m in 0.5% DMSO (final concentrations), whereas the vehicle was a mixture of saline and 0.5% DMSO.

Techniques: Activity Assay, Expressing, Injection, Inhibition

Journal: Learning & Memory

Article Title: Different phases of long-term memory require distinct temporal patterns of PKA activity after single-trial classical conditioning

doi: 10.1101/lm.1088408

Figure Lengend Snippet: A conserved functional PKA catalytic subunit (PKA C) is present in the Lymnaea CNS. ( A ) cAMP stimulates PKA C activity in Lymnaea nervous system extracts ( Ai ) and purified PKA fractions ( Aii ), which can be blocked by KT5720 or PKAI. The whole CNS data (mean ±SEM) come from experiments with single brains ( n = 19). *, P

Article Snippet: Snails from one of the KT5720 and vehicle-injected groups each were tested at 6 h while the remaining KT5720 and vehicle-injected snails were tested at 24 h post-training for their feeding responses to the CS.

Techniques: Functional Assay, Activity Assay, Purification

Journal: JCI Insight

Article Title: Striatal TRPV1 activation by acetaminophen ameliorates dopamine D2 receptor antagonist–induced orofacial dyskinesia

doi: 10.1172/jci.insight.145632

Figure Lengend Snippet: Effects of intracerebral AM404 on haloperidol-induced VCMs in rodents. ( A ) Rats ( n = 5–7 per group) were treated daily with oral haloperidol (1 mg/kg/d) for 21 days and received AM404 (0, 10, or 50 nmol) via intracerebroventricular injection at 23 hours after the last administration of haloperidol. The number of VCMs was measured for 3 minutes starting 30 minutes after the administration of AM404. After the experiment, the injection sites were confirmed by injecting Evans blue through the same cannula. A representative image of consecutive coronal sections is shown (43 mm wide in actual size). Individual data are shown with the mean ± SEM. Statistical significance was tested using 1-way ANOVA with post hoc Tukey’s test. * P

Article Snippet: Haloperidol was purchased from Tokyo Chemical Industry; acetaminophen and capsaicin from Nacalai Tesque; AM404 from Alomone Labs; methamphetamine from Sumitomo Dainippon Pharma; CNO from Cayman Chemical; DL-2-amino-5-phosphonopentanoic acid (DL-APV) from MilliporeSigma; dinitroquinoxaline-2,3(1H,4H)-dione (DNQX) from Tocris Bioscience (Bio-Techne); bicuculline from Enzo Life Science; and pentobarbital from Kyoritsu Seiyaku.

Techniques: Injection

Journal: JCI Insight

Article Title: Striatal TRPV1 activation by acetaminophen ameliorates dopamine D2 receptor antagonist–induced orofacial dyskinesia

doi: 10.1172/jci.insight.145632

Figure Lengend Snippet: Effects of the dorsal striatal injection of AM404 and capsaicin on haloperidol-induced VCMs in WT and TRPV1-KO mice. WT and TRPV1-KO mice were treated daily with orally administrated haloperidol (2 mg/kg/d) for 21 days. On day 22, after VCMs were counted for 5 minutes as an initial baseline, half of the mice received vehicle (group 1), and the rest received AM404 (1 pmol/side) or capsaicin (10 ng/side) (group 2) through a preimplanted cannula in the dorsal striatum. Five minutes later, the number of VCMs was counted for another 5 minutes to determine the effect of the injected drug. The daily haloperidol treatment was continued for 4 more days; on day 26, the second set of VCM measurements was performed in a crossover design, with AM404 or capsaicin injection in group 1 and vehicle injection in group 2. ( A and B ) Effects of the dorsal striatal injection of AM404 on haloperidol-induced VCMs ( n = 9 per group). ( C and D ) Effects of the dorsal striatal injection of capsaicin on haloperidol-induced VCMs ( n = 7 per group). Changes in the number of VCMs are represented for individual mice on days 22 and 26 as percentages of the baseline VCM count. Open and filled circles indicate the results from groups 1 and 2, respectively. Statistical significance was tested using repeated measures 2-way ANOVA with post hoc multiple comparisons; * P

Article Snippet: Haloperidol was purchased from Tokyo Chemical Industry; acetaminophen and capsaicin from Nacalai Tesque; AM404 from Alomone Labs; methamphetamine from Sumitomo Dainippon Pharma; CNO from Cayman Chemical; DL-2-amino-5-phosphonopentanoic acid (DL-APV) from MilliporeSigma; dinitroquinoxaline-2,3(1H,4H)-dione (DNQX) from Tocris Bioscience (Bio-Techne); bicuculline from Enzo Life Science; and pentobarbital from Kyoritsu Seiyaku.

Techniques: Injection, Mouse Assay