Journal: Frontiers in Physiology
Article Title: Contributions of CaV1.3 Channels to Ca2+ Current and Ca2+-Activated BK Current in the Suprachiasmatic Nucleus
Figure Lengend Snippet: Effects of Ca 2+ chelators and Ca 2+ channel inhibitors on BK currents recorded from SCN neurons during the day and night. Paxilline-sensitive macroscopic BK currents were recorded from C57BL6 WT (A–E) and Ca V 1.3 WT (F) SCNs. All intracellular solutions in this study were made with 0.9 mM EGTA, except where 5 mM BAPTA was substituted (A–E) . Currents were elicited from a holding potential of −90 mV by 150-ms voltage steps from −110 to +90 mV in +20-mV increments. (A,B) Representative BK currents from −90 to +90 mV are shown from day (A) and night (B) SCN neurons. (C,D) Current-voltage plot comparing BK current density recorded in either control EGTA or BAPTA during the day (C) and night (D) . (E) Summary of BK current density at +90 mV recorded with control EGTA or BAPTA with Ca 2+ channel inhibitors 10 μM nimodipine (Nim) during the day, or 10 μM dantrolene (Dan) at night. In EGTA, Nim decreased BK currents compared to controls ( P = 0.0008), with no significant difference in BAPTA. At night, BK currents were decreased with BAPTA compared to control EGTA conditions ( P
Article Snippet: Drugs used in these experiments were: L-type Ca2+ channel inhibitor nimodipine (Nim, 10 μM, Alomone Labs, Jerusalem, Israel, #N-150), Ryanodine Receptor inhibitor dantroline (Dan, 10 μM, Sigma, #D9175), BK current inhibitor Paxilline (Pax, 10 μM, Alomone Labs, Jerusalem, Israel, #P-450) and Sodium channel inhibitor tetrodotoxin (TTX, 1 μM, Alomone Labs, Jerusalem, Israel, #T-550).