cryo tem microscope  (Thermo Fisher)

Journal: Nanomaterials

Article Title: Experimental Study on the Drag Reduction Performance of Clear Fracturing Fluid Using Wormlike Surfactant Micelles and Magnetic Nanoparticles under a Magnetic Field

doi: 10.3390/nano11040885

Figure Lengend Snippet: Cryo-TEM micrographs of the WLMs with different FE-NP concentrations: ( a ) 0 wt%; ( b ) 0.1 wt%; ( c ) 0.3 wt%; and ( d ) 0.5 wt%.

Article Snippet: Then, the frozen specimen was transferred into a cryo-holder and was observed with cryo-TEM (Krios G4, Thermo Fisher Scientific Inc., Waltham, MA, USA).

Techniques: Transmission Electron Microscopy

Journal: Scientific Reports

Article Title: Proteomic profile of extracellular vesicles released by Lactiplantibacillus plantarum BGAN8 and their internalization by non-polarized HT29 cell line

doi: 10.1038/s41598-020-78920-z

Figure Lengend Snippet: Extracellular vesicles purified from L. plantarum BGAN8 cell-free supernatants. ( A ) EVs visualized by cryo-transmission electron microscopy (bar 200 nm); size of the particles (from up to bottom and left to right): 115.2 nm, 69.5 nm, 91.3 nm and 40.6 nm). ( B ) Size distribution of the EVs according to the diameter range (nm) measured under the cryo-TEM microscope.

Article Snippet: The vitrified sample was stored in liquid nitrogen until its observation in the cryo-TEM microscope.

Techniques: Purification, Transmission Assay, Electron Microscopy, Transmission Electron Microscopy, Microscopy

Journal: Mucosal Immunology

Article Title: Intestinal epithelial NAIP/NLRC4 restricts systemic dissemination of the adapted pathogen Salmonella Typhimurium due to site-specific bacterial PAMP expression

doi: 10.1038/s41385-019-0247-0

Figure Lengend Snippet: S . Tm expresses PAMPs recognized by NAIP/NLRC4 in the cecal epithelium, but downregulates them upon traversal. a S . Tm with potentially detached (left/upper panel) and attached (right/lower panel) flagella can be found inside SCVs. Shown are 15 nm slices of cryotomograms of cryo-FIB milled HeLa cells and the respective segmentations (representative for six tomograms; frozen at 1 hpi and MOI 300). OM: outer membrane. IM: inner membrane. SCV: Salmonella containing vacuole. hCP: host cell cytoplasm. Scale bar, 100 nm. b S . Tm as a rule expresses SPI-1 in the cecum (12 hpi, n total S . Tm =322) and downregulates the expression in the spleen (3 dpi, n total S . Tm =35) of JH −/− mice. c The fraction of SPI-1 + S . Tm within the cecal mucosa decreases upon traversal to the lamina propria (n total S . Tm epithelium =253; n total S . Tm lamina propria =69). Quantifications shown in b and c are based on fluorescence microscopy of S . Tm SPI-1-GFP expressing cytoplasmic GFP under the prgH -promotor and an LPS immunostaining. d Representative images of tissue sections (cecum, spleen), which were used for the quantifications in b and c . e Flagella are prominent in the cecal mucosa (12 hpi, n total S . Tm =341), while they are completely absent in the spleen (3 dpi, n total S . Tm =50) of JH −/− mice. Quantifications were performed by fluorescence immunostaining of FliC. f Similar to SPI-1 ( c ) expression, flagella are less evident in the lamina propria (n total S . Tm lamina propria =39) than within IECs (n total S . Tm epithelium =302). g Representative images of tissue sections (cecum, spleen), which were used for the quantifications in e and f . White arrowheads: flagella; white arrows: SPI-1-GFP + S . Tm (epithelium and lamina propria); E: epithelium; L: lumen; LP: lamina propria. Black bar: Median. Statistical analysis: Mann-Whitney- U Test, p -values indicated. Scale bars, 10 µm.

Article Snippet: Cryo-focused ion beam milling Cryo-focused ion beam (cryoFIB) milling was used to prepare samples of plunge-frozen infected HeLa cells that could then be imaged by cryo-electron tomography.

Techniques: Expressing, Mouse Assay, Fluorescence, Microscopy, Immunostaining, MANN-WHITNEY

Journal: bioRxiv

Article Title: Demonstration of electron diffraction from membrane protein crystals grown in a lipidic mesophase after lamella preparation by focused ion beam milling at cryogenic temperatures

doi: 10.1101/2020.07.03.186049

Figure Lengend Snippet: Electron diffraction experiment on a 210 nm thick lamella of bacteriorhodopsin, using a 200-kV cryo-TEM microscope. (a) TEM micrograph of the FIB-machined lamella of the BR crystal. Electron diffraction signal was collected from a 1.4-µm area of the lamella, indicated by a red circle with a cross. The scale bar on (a) corresponds to 5 µm. (b) The 200-kV electron diffraction pattern obtained from the area indicated in (a). (c) The electron diffraction image is corrected by subtraction of local moving-average background, calculated with the Adxv program ( Adxv, 2013 ). The inset shows a close up of the electron diffraction pattern. (d) Diffraction peaks were automatically picked up by the Adxv software, and the diffraction peaks in the resolution shell of 2.7 Å - 2.45 Å had a mean signal-to-noise ratio

Article Snippet: This implies that, in order to collect ED data suitable for high-resolution structural determination with the experimental system used in the present article, at least 3-6 different lamellae have to be produced and have to survive transfer from the cryo-FIB instrument to the cryo-TEM microscope.

Techniques: Transmission Electron Microscopy, Microscopy, Software