transmission electron microscope  (Thermo Fisher)

Journal: Nanomaterials

Article Title: Experimental Study on the Drag Reduction Performance of Clear Fracturing Fluid Using Wormlike Surfactant Micelles and Magnetic Nanoparticles under a Magnetic Field

doi: 10.3390/nano11040885

Figure Lengend Snippet: Cryo-TEM micrographs of the WLMs with different FE-NP concentrations: ( a ) 0 wt%; ( b ) 0.1 wt%; ( c ) 0.3 wt%; and ( d ) 0.5 wt%.

Article Snippet: Then, the frozen specimen was transferred into a cryo-holder and was observed with cryo-TEM (Krios G4, Thermo Fisher Scientific Inc., Waltham, MA, USA).

Techniques: Transmission Electron Microscopy

Journal: Cell

Article Title: Protective prototype-Beta and Delta-Omicron chimeric RBD-dimer vaccines against SARS-CoV-2

doi: 10.1016/j.cell.2022.04.029

Figure Lengend Snippet: Cryo-EM analysis of CB6 Fab in complexed with Delta-Omicron chimeric RBD-dimer and representative cryo-EM 2D class average images of RBD-dimers bound to CB6 Fabs, related to Figure 1 (A) Flow chart of cryo-EM data processing for Delta-Omicron chimeric RBD-dimer bound to CB6. (B) Euler angle distribution of the final reconstruction. (C) The FSC curve for the reconstruction, the FSC 0.5 cutoff value is indicated by red dashed line. (D) Representative cryo-EM 2D class average images of prototype RBD-dimer bound to two CB6 Fabs. (E) Representative cryo-EM 2D class average images of prototype-Beta chimeric RBD-dimer bound to one CB6 Fab. (F) Representative cryo-EM 2D class average images of Delta-Omicron chimeric RBD-dimer bound to one CB6 Fab.

Article Snippet: The frozen grides were loaded onto a Titan Krios cryo-transmission electron microscope (Thermo Fisher) that is equipped with a BioQuantum energy filter (Gatan), operated at 300 kV for data collection.

Techniques: Cryo-EM Sample Prep

Journal: Cell

Article Title: Protective prototype-Beta and Delta-Omicron chimeric RBD-dimer vaccines against SARS-CoV-2

doi: 10.1016/j.cell.2022.04.029

Figure Lengend Snippet: Cryo-EM analysis of CB6 Fab in complexed with prototype RBD-dimer and prototype-Beta chimeric RBD-dimer, respectively, related to Figure 1 (A–C) Cryo-EM analysis of CB6 Fab in complexed with prototype RBD-dimer. (D–F) Cryo-EM analysis of CB6 Fab in complexed with prototype-Beta chimeric RBD-dimer. (A and D) Flow chart of cryo-EM data processing for RBD-dimers bound to CB6. (B and E) Euler angle distribution of the final reconstruction. (C and F) The FSC curve for the reconstruction, the FSC 0.5 cutoff value is indicated by red dashed line.

Article Snippet: The frozen grides were loaded onto a Titan Krios cryo-transmission electron microscope (Thermo Fisher) that is equipped with a BioQuantum energy filter (Gatan), operated at 300 kV for data collection.

Techniques: Cryo-EM Sample Prep

Journal: Materials Today Bio

Article Title: The involvement of extracellular vesicles in the transcytosis of nanoliposomes through brain endothelial cells, and the impact of liposomal pH-sensitivity

doi: 10.1016/j.mtbio.2022.100212

Figure Lengend Snippet: Physicochemical properties of isolated vesicles from hBMECs following treatment with GSH-PEG-L and GSH-PEG-pSL for 2 ​h versus EVs from non-treated cells (control). A) the particle numbers in the sEV and mEV fractions; B C) the FIs of Rh-PE (B) and calcein (C) in the EV lysates; D) Vesicle size, zeta potential and morphology under cryo-TEM. Vesicles secreted from cells were fractioned at various centrifugation speeds of 2000 g– defined as mEV 2000 g fraction, 10000 ​g – mEVs and 100,000 ​g – sEVs.

Article Snippet: The morphology and lamellar structure of frozen samples of the isolated vesicles was viewed with Cryo-TEM (Tecnai 12 electron microscope, FEI, Hillsboro, USA) as previously described [ , ].

Techniques: Isolation, Transmission Electron Microscopy, Centrifugation