cryo tem microscope  (Thermo Fisher)


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    Structured Review

    Thermo Fisher cryo tem microscope
    Extracellular vesicles purified from L. plantarum BGAN8 cell-free supernatants. ( A ) EVs visualized by <t>cryo-transmission</t> electron microscopy (bar 200 nm); size of the particles (from up to bottom and left to right): 115.2 nm, 69.5 nm, 91.3 nm and 40.6 nm). ( B ) Size distribution of the EVs according to the diameter range (nm) measured under the <t>cryo-TEM</t> microscope.
    Cryo Tem Microscope, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/cryo tem microscope/product/Thermo Fisher
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    cryo tem microscope - by Bioz Stars, 2021-06
    99/100 stars

    Images

    1) Product Images from "Proteomic profile of extracellular vesicles released by Lactiplantibacillus plantarum BGAN8 and their internalization by non-polarized HT29 cell line"

    Article Title: Proteomic profile of extracellular vesicles released by Lactiplantibacillus plantarum BGAN8 and their internalization by non-polarized HT29 cell line

    Journal: Scientific Reports

    doi: 10.1038/s41598-020-78920-z

    Extracellular vesicles purified from L. plantarum BGAN8 cell-free supernatants. ( A ) EVs visualized by cryo-transmission electron microscopy (bar 200 nm); size of the particles (from up to bottom and left to right): 115.2 nm, 69.5 nm, 91.3 nm and 40.6 nm). ( B ) Size distribution of the EVs according to the diameter range (nm) measured under the cryo-TEM microscope.
    Figure Legend Snippet: Extracellular vesicles purified from L. plantarum BGAN8 cell-free supernatants. ( A ) EVs visualized by cryo-transmission electron microscopy (bar 200 nm); size of the particles (from up to bottom and left to right): 115.2 nm, 69.5 nm, 91.3 nm and 40.6 nm). ( B ) Size distribution of the EVs according to the diameter range (nm) measured under the cryo-TEM microscope.

    Techniques Used: Purification, Transmission Assay, Electron Microscopy, Transmission Electron Microscopy, Microscopy

    2) Product Images from "Intestinal epithelial NAIP/NLRC4 restricts systemic dissemination of the adapted pathogen Salmonella Typhimurium due to site-specific bacterial PAMP expression"

    Article Title: Intestinal epithelial NAIP/NLRC4 restricts systemic dissemination of the adapted pathogen Salmonella Typhimurium due to site-specific bacterial PAMP expression

    Journal: Mucosal Immunology

    doi: 10.1038/s41385-019-0247-0

    S . Tm expresses PAMPs recognized by NAIP/NLRC4 in the cecal epithelium, but downregulates them upon traversal. a S . Tm with potentially detached (left/upper panel) and attached (right/lower panel) flagella can be found inside SCVs. Shown are 15 nm slices of cryotomograms of cryo-FIB milled HeLa cells and the respective segmentations (representative for six tomograms; frozen at 1 hpi and MOI 300). OM: outer membrane. IM: inner membrane. SCV: Salmonella containing vacuole. hCP: host cell cytoplasm. Scale bar, 100 nm. b S . Tm as a rule expresses SPI-1 in the cecum (12 hpi, n total S . Tm =322) and downregulates the expression in the spleen (3 dpi, n total S . Tm =35) of JH −/− mice. c The fraction of SPI-1 + S . Tm within the cecal mucosa decreases upon traversal to the lamina propria (n total S . Tm epithelium =253; n total S . Tm lamina propria =69). Quantifications shown in b and c are based on fluorescence microscopy of S . Tm SPI-1-GFP expressing cytoplasmic GFP under the prgH -promotor and an LPS immunostaining. d Representative images of tissue sections (cecum, spleen), which were used for the quantifications in b and c . e Flagella are prominent in the cecal mucosa (12 hpi, n total S . Tm =341), while they are completely absent in the spleen (3 dpi, n total S . Tm =50) of JH −/− mice. Quantifications were performed by fluorescence immunostaining of FliC. f Similar to SPI-1 ( c ) expression, flagella are less evident in the lamina propria (n total S . Tm lamina propria =39) than within IECs (n total S . Tm epithelium =302). g Representative images of tissue sections (cecum, spleen), which were used for the quantifications in e and f . White arrowheads: flagella; white arrows: SPI-1-GFP + S . Tm (epithelium and lamina propria); E: epithelium; L: lumen; LP: lamina propria. Black bar: Median. Statistical analysis: Mann-Whitney- U Test, p -values indicated. Scale bars, 10 µm.
    Figure Legend Snippet: S . Tm expresses PAMPs recognized by NAIP/NLRC4 in the cecal epithelium, but downregulates them upon traversal. a S . Tm with potentially detached (left/upper panel) and attached (right/lower panel) flagella can be found inside SCVs. Shown are 15 nm slices of cryotomograms of cryo-FIB milled HeLa cells and the respective segmentations (representative for six tomograms; frozen at 1 hpi and MOI 300). OM: outer membrane. IM: inner membrane. SCV: Salmonella containing vacuole. hCP: host cell cytoplasm. Scale bar, 100 nm. b S . Tm as a rule expresses SPI-1 in the cecum (12 hpi, n total S . Tm =322) and downregulates the expression in the spleen (3 dpi, n total S . Tm =35) of JH −/− mice. c The fraction of SPI-1 + S . Tm within the cecal mucosa decreases upon traversal to the lamina propria (n total S . Tm epithelium =253; n total S . Tm lamina propria =69). Quantifications shown in b and c are based on fluorescence microscopy of S . Tm SPI-1-GFP expressing cytoplasmic GFP under the prgH -promotor and an LPS immunostaining. d Representative images of tissue sections (cecum, spleen), which were used for the quantifications in b and c . e Flagella are prominent in the cecal mucosa (12 hpi, n total S . Tm =341), while they are completely absent in the spleen (3 dpi, n total S . Tm =50) of JH −/− mice. Quantifications were performed by fluorescence immunostaining of FliC. f Similar to SPI-1 ( c ) expression, flagella are less evident in the lamina propria (n total S . Tm lamina propria =39) than within IECs (n total S . Tm epithelium =302). g Representative images of tissue sections (cecum, spleen), which were used for the quantifications in e and f . White arrowheads: flagella; white arrows: SPI-1-GFP + S . Tm (epithelium and lamina propria); E: epithelium; L: lumen; LP: lamina propria. Black bar: Median. Statistical analysis: Mann-Whitney- U Test, p -values indicated. Scale bars, 10 µm.

    Techniques Used: Expressing, Mouse Assay, Fluorescence, Microscopy, Immunostaining, MANN-WHITNEY

    3) Product Images from "Demonstration of electron diffraction from membrane protein crystals grown in a lipidic mesophase after lamella preparation by focused ion beam milling at cryogenic temperatures"

    Article Title: Demonstration of electron diffraction from membrane protein crystals grown in a lipidic mesophase after lamella preparation by focused ion beam milling at cryogenic temperatures

    Journal: bioRxiv

    doi: 10.1101/2020.07.03.186049

    Electron diffraction experiment on a 210 nm thick lamella of bacteriorhodopsin, using a 200-kV cryo-TEM microscope. (a) TEM micrograph of the FIB-machined lamella of the BR crystal. Electron diffraction signal was collected from a 1.4-µm area of the lamella, indicated by a red circle with a cross. The scale bar on (a) corresponds to 5 µm. (b) The 200-kV electron diffraction pattern obtained from the area indicated in (a). (c) The electron diffraction image is corrected by subtraction of local moving-average background, calculated with the Adxv program ( Adxv, 2013 ). The inset shows a close up of the electron diffraction pattern. (d) Diffraction peaks were automatically picked up by the Adxv software, and the diffraction peaks in the resolution shell of 2.7 Å - 2.45 Å had a mean signal-to-noise ratio
    Figure Legend Snippet: Electron diffraction experiment on a 210 nm thick lamella of bacteriorhodopsin, using a 200-kV cryo-TEM microscope. (a) TEM micrograph of the FIB-machined lamella of the BR crystal. Electron diffraction signal was collected from a 1.4-µm area of the lamella, indicated by a red circle with a cross. The scale bar on (a) corresponds to 5 µm. (b) The 200-kV electron diffraction pattern obtained from the area indicated in (a). (c) The electron diffraction image is corrected by subtraction of local moving-average background, calculated with the Adxv program ( Adxv, 2013 ). The inset shows a close up of the electron diffraction pattern. (d) Diffraction peaks were automatically picked up by the Adxv software, and the diffraction peaks in the resolution shell of 2.7 Å - 2.45 Å had a mean signal-to-noise ratio

    Techniques Used: Transmission Electron Microscopy, Microscopy, Software

    Related Articles

    Infection:

    Article Title: Intestinal epithelial NAIP/NLRC4 restricts systemic dissemination of the adapted pathogen Salmonella Typhimurium due to site-specific bacterial PAMP expression
    Article Snippet: Grids were plunge-frozen in liquid ethane-propane (37%/63%) using a Vitrobot (Thermo Fisher) and stored in liquid nitrogen. .. Cryo-focused ion beam milling Cryo-focused ion beam (cryoFIB) milling was used to prepare samples of plunge-frozen infected HeLa cells that could then be imaged by cryo-electron tomography. .. Frozen grids with infected HeLa cells were prepared and processed as previously described.

    other:

    Article Title: In Situ Conformational Changes of the Escherichia coli Serine Chemoreceptor in Different Signaling States
    Article Snippet: Cryo-electron tomography.

    Article Title: Quality Assessment and Comparison of Plasma-Derived Extracellular Vesicles Separated by Three Commercial Kits for Prostate Cancer Diagnosis
    Article Snippet: The vitrified samples were stored in liquid nitrogen (−196 °C) prior to cryo-TEM analysis.

    Sequencing:

    Article Title: Light-driven ATP production promotes mRNA biosynthesis inside hybrid multi-compartment artificial protocells
    Article Snippet: The particles were imaged using a Tecnai F20 microscope (Thermo Fisher Scientific - US), equipped with a Schottky Field Emission electron source operated at an acceleration voltage of 200 kV, a US1000 2k × 2k Gatan CCD camera and a FEI retractable cryo box to limit water sublimation from the vitrified sample and its recrystallization on the specimen surface. .. For the cryo-electron tomography the tilt series were collected by tilting the vitrified sample over ± 60° with the following tilt sequence: starting from 0° to ± 48 with a tilt step of 3°; then, from ± 48° to ± 60°with a tilt step of 2°. .. The cryo-EM imaging was carried out at a final object pixel of 3.6 Å, with a total dose of ∼60 e− /Å2 in order to limit specimen damage.

    Produced:

    Article Title: Demonstration of electron diffraction from membrane protein crystals grown in a lipidic mesophase after lamella preparation by focused ion beam milling at cryogenic temperatures
    Article Snippet: .. This implies that, in order to collect ED data suitable for high-resolution structural determination with the experimental system used in the present article, at least 3-6 different lamellae have to be produced and have to survive transfer from the cryo-FIB instrument to the cryo-TEM microscope. .. Cryo-FIB milling is time consuming (∼5 h per lamella in our case) and the transfer-surviving rate was about ∼50% in our case (see also ( ; )), making the overall sample preparation cumbersome.

    Microscopy:

    Article Title: Demonstration of electron diffraction from membrane protein crystals grown in a lipidic mesophase after lamella preparation by focused ion beam milling at cryogenic temperatures
    Article Snippet: .. This implies that, in order to collect ED data suitable for high-resolution structural determination with the experimental system used in the present article, at least 3-6 different lamellae have to be produced and have to survive transfer from the cryo-FIB instrument to the cryo-TEM microscope. .. Cryo-FIB milling is time consuming (∼5 h per lamella in our case) and the transfer-surviving rate was about ∼50% in our case (see also ( ; )), making the overall sample preparation cumbersome.

    Article Title: Proteomic profile of extracellular vesicles released by Lactiplantibacillus plantarum BGAN8 and their internalization by non-polarized HT29 cell line
    Article Snippet: .. The vitrified sample was stored in liquid nitrogen until its observation in the cryo-TEM microscope. .. Plunge-frozen sample was transferred to a Tecnai F20 EM (FEI Companys) using a cryo-holder system (Gatan, Pleasanton, USA).

    Article Title: Development of single nanometer-sized ultrafine oxygen bubbles to overcome the hypoxia-induced resistance to radiation therapy via the suppression of hypoxia-inducible factor-1α
    Article Snippet: Pure water with or without diluted nanobubble was rapidly frozen using Vitrobot Mark IV (FEI Co., Ltd., Hillsboro, OR, USA). .. Nanobubbles embedded in amorphous ice at a sample temperature of about −193°C were directly observed using a cryo-transmission electron microscope Titan Krios (FEI Co., Ltd.). ..

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    Thermo Fisher cryo tem
    <t>Cryo-TEM</t> micrographs of the WLMs with different FE-NP concentrations: ( a ) 0 wt%; ( b ) 0.1 wt%; ( c ) 0.3 wt%; and ( d ) 0.5 wt%.
    Cryo Tem, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/cryo tem/product/Thermo Fisher
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    cryo tem - by Bioz Stars, 2021-06
    86/100 stars
      Buy from Supplier

    99
    Thermo Fisher cryo tem microscope
    Extracellular vesicles purified from L. plantarum BGAN8 cell-free supernatants. ( A ) EVs visualized by <t>cryo-transmission</t> electron microscopy (bar 200 nm); size of the particles (from up to bottom and left to right): 115.2 nm, 69.5 nm, 91.3 nm and 40.6 nm). ( B ) Size distribution of the EVs according to the diameter range (nm) measured under the <t>cryo-TEM</t> microscope.
    Cryo Tem Microscope, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/cryo tem microscope/product/Thermo Fisher
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    cryo tem microscope - by Bioz Stars, 2021-06
    99/100 stars
      Buy from Supplier

    Image Search Results


    Cryo-TEM micrographs of the WLMs with different FE-NP concentrations: ( a ) 0 wt%; ( b ) 0.1 wt%; ( c ) 0.3 wt%; and ( d ) 0.5 wt%.

    Journal: Nanomaterials

    Article Title: Experimental Study on the Drag Reduction Performance of Clear Fracturing Fluid Using Wormlike Surfactant Micelles and Magnetic Nanoparticles under a Magnetic Field

    doi: 10.3390/nano11040885

    Figure Lengend Snippet: Cryo-TEM micrographs of the WLMs with different FE-NP concentrations: ( a ) 0 wt%; ( b ) 0.1 wt%; ( c ) 0.3 wt%; and ( d ) 0.5 wt%.

    Article Snippet: Then, the frozen specimen was transferred into a cryo-holder and was observed with cryo-TEM (Krios G4, Thermo Fisher Scientific Inc., Waltham, MA, USA).

    Techniques: Transmission Electron Microscopy

    Extracellular vesicles purified from L. plantarum BGAN8 cell-free supernatants. ( A ) EVs visualized by cryo-transmission electron microscopy (bar 200 nm); size of the particles (from up to bottom and left to right): 115.2 nm, 69.5 nm, 91.3 nm and 40.6 nm). ( B ) Size distribution of the EVs according to the diameter range (nm) measured under the cryo-TEM microscope.

    Journal: Scientific Reports

    Article Title: Proteomic profile of extracellular vesicles released by Lactiplantibacillus plantarum BGAN8 and their internalization by non-polarized HT29 cell line

    doi: 10.1038/s41598-020-78920-z

    Figure Lengend Snippet: Extracellular vesicles purified from L. plantarum BGAN8 cell-free supernatants. ( A ) EVs visualized by cryo-transmission electron microscopy (bar 200 nm); size of the particles (from up to bottom and left to right): 115.2 nm, 69.5 nm, 91.3 nm and 40.6 nm). ( B ) Size distribution of the EVs according to the diameter range (nm) measured under the cryo-TEM microscope.

    Article Snippet: The vitrified sample was stored in liquid nitrogen until its observation in the cryo-TEM microscope.

    Techniques: Purification, Transmission Assay, Electron Microscopy, Transmission Electron Microscopy, Microscopy

    S . Tm expresses PAMPs recognized by NAIP/NLRC4 in the cecal epithelium, but downregulates them upon traversal. a S . Tm with potentially detached (left/upper panel) and attached (right/lower panel) flagella can be found inside SCVs. Shown are 15 nm slices of cryotomograms of cryo-FIB milled HeLa cells and the respective segmentations (representative for six tomograms; frozen at 1 hpi and MOI 300). OM: outer membrane. IM: inner membrane. SCV: Salmonella containing vacuole. hCP: host cell cytoplasm. Scale bar, 100 nm. b S . Tm as a rule expresses SPI-1 in the cecum (12 hpi, n total S . Tm =322) and downregulates the expression in the spleen (3 dpi, n total S . Tm =35) of JH −/− mice. c The fraction of SPI-1 + S . Tm within the cecal mucosa decreases upon traversal to the lamina propria (n total S . Tm epithelium =253; n total S . Tm lamina propria =69). Quantifications shown in b and c are based on fluorescence microscopy of S . Tm SPI-1-GFP expressing cytoplasmic GFP under the prgH -promotor and an LPS immunostaining. d Representative images of tissue sections (cecum, spleen), which were used for the quantifications in b and c . e Flagella are prominent in the cecal mucosa (12 hpi, n total S . Tm =341), while they are completely absent in the spleen (3 dpi, n total S . Tm =50) of JH −/− mice. Quantifications were performed by fluorescence immunostaining of FliC. f Similar to SPI-1 ( c ) expression, flagella are less evident in the lamina propria (n total S . Tm lamina propria =39) than within IECs (n total S . Tm epithelium =302). g Representative images of tissue sections (cecum, spleen), which were used for the quantifications in e and f . White arrowheads: flagella; white arrows: SPI-1-GFP + S . Tm (epithelium and lamina propria); E: epithelium; L: lumen; LP: lamina propria. Black bar: Median. Statistical analysis: Mann-Whitney- U Test, p -values indicated. Scale bars, 10 µm.

    Journal: Mucosal Immunology

    Article Title: Intestinal epithelial NAIP/NLRC4 restricts systemic dissemination of the adapted pathogen Salmonella Typhimurium due to site-specific bacterial PAMP expression

    doi: 10.1038/s41385-019-0247-0

    Figure Lengend Snippet: S . Tm expresses PAMPs recognized by NAIP/NLRC4 in the cecal epithelium, but downregulates them upon traversal. a S . Tm with potentially detached (left/upper panel) and attached (right/lower panel) flagella can be found inside SCVs. Shown are 15 nm slices of cryotomograms of cryo-FIB milled HeLa cells and the respective segmentations (representative for six tomograms; frozen at 1 hpi and MOI 300). OM: outer membrane. IM: inner membrane. SCV: Salmonella containing vacuole. hCP: host cell cytoplasm. Scale bar, 100 nm. b S . Tm as a rule expresses SPI-1 in the cecum (12 hpi, n total S . Tm =322) and downregulates the expression in the spleen (3 dpi, n total S . Tm =35) of JH −/− mice. c The fraction of SPI-1 + S . Tm within the cecal mucosa decreases upon traversal to the lamina propria (n total S . Tm epithelium =253; n total S . Tm lamina propria =69). Quantifications shown in b and c are based on fluorescence microscopy of S . Tm SPI-1-GFP expressing cytoplasmic GFP under the prgH -promotor and an LPS immunostaining. d Representative images of tissue sections (cecum, spleen), which were used for the quantifications in b and c . e Flagella are prominent in the cecal mucosa (12 hpi, n total S . Tm =341), while they are completely absent in the spleen (3 dpi, n total S . Tm =50) of JH −/− mice. Quantifications were performed by fluorescence immunostaining of FliC. f Similar to SPI-1 ( c ) expression, flagella are less evident in the lamina propria (n total S . Tm lamina propria =39) than within IECs (n total S . Tm epithelium =302). g Representative images of tissue sections (cecum, spleen), which were used for the quantifications in e and f . White arrowheads: flagella; white arrows: SPI-1-GFP + S . Tm (epithelium and lamina propria); E: epithelium; L: lumen; LP: lamina propria. Black bar: Median. Statistical analysis: Mann-Whitney- U Test, p -values indicated. Scale bars, 10 µm.

    Article Snippet: Cryo-focused ion beam milling Cryo-focused ion beam (cryoFIB) milling was used to prepare samples of plunge-frozen infected HeLa cells that could then be imaged by cryo-electron tomography.

    Techniques: Expressing, Mouse Assay, Fluorescence, Microscopy, Immunostaining, MANN-WHITNEY

    Electron diffraction experiment on a 210 nm thick lamella of bacteriorhodopsin, using a 200-kV cryo-TEM microscope. (a) TEM micrograph of the FIB-machined lamella of the BR crystal. Electron diffraction signal was collected from a 1.4-µm area of the lamella, indicated by a red circle with a cross. The scale bar on (a) corresponds to 5 µm. (b) The 200-kV electron diffraction pattern obtained from the area indicated in (a). (c) The electron diffraction image is corrected by subtraction of local moving-average background, calculated with the Adxv program ( Adxv, 2013 ). The inset shows a close up of the electron diffraction pattern. (d) Diffraction peaks were automatically picked up by the Adxv software, and the diffraction peaks in the resolution shell of 2.7 Å - 2.45 Å had a mean signal-to-noise ratio

    Journal: bioRxiv

    Article Title: Demonstration of electron diffraction from membrane protein crystals grown in a lipidic mesophase after lamella preparation by focused ion beam milling at cryogenic temperatures

    doi: 10.1101/2020.07.03.186049

    Figure Lengend Snippet: Electron diffraction experiment on a 210 nm thick lamella of bacteriorhodopsin, using a 200-kV cryo-TEM microscope. (a) TEM micrograph of the FIB-machined lamella of the BR crystal. Electron diffraction signal was collected from a 1.4-µm area of the lamella, indicated by a red circle with a cross. The scale bar on (a) corresponds to 5 µm. (b) The 200-kV electron diffraction pattern obtained from the area indicated in (a). (c) The electron diffraction image is corrected by subtraction of local moving-average background, calculated with the Adxv program ( Adxv, 2013 ). The inset shows a close up of the electron diffraction pattern. (d) Diffraction peaks were automatically picked up by the Adxv software, and the diffraction peaks in the resolution shell of 2.7 Å - 2.45 Å had a mean signal-to-noise ratio

    Article Snippet: This implies that, in order to collect ED data suitable for high-resolution structural determination with the experimental system used in the present article, at least 3-6 different lamellae have to be produced and have to survive transfer from the cryo-FIB instrument to the cryo-TEM microscope.

    Techniques: Transmission Electron Microscopy, Microscopy, Software