cryo tem microscope  (Thermo Fisher)


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    Name:
    Krios G4 Cryo TEM
    Description:
    The new Thermo Scientific Krios G4 Cryo Transmission Electron Microscope Cryo TEM enables you to unravel life at the molecular level easier faster and more reliably than ever before The most compact TEM in its class the Krios G4 Cryo TEM consists of a highly stable 300 kV TEM platform and the industry leading Autoloader cryogenic sample manipulation robot making it ideally suited for automated applications such as single particle analysis SPA cryo electron tomography cryoET and micro electron diffraction MicroED Through a thorough redesign of the mechanical base frame and system enclosure the microscope height has been reduced to below 3 meters which allows for instrument installation in labs with a ceiling height below 3 04m 10 ft thereby avoiding costly room renovations Designed in connectivity ensures a robust and risk free pathway throughout the entire workflow from sample preparation and optimization to image acquisition and data processing
    Catalog Number:
    KRIOSG4TEM
    Price:
    None
    Category:
    Transmssion Electron Microscope TEM
    Applications:
    single particle analysis, cryo-tomography, microED
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    Structured Review

    Thermo Fisher cryo tem microscope
    Extracellular vesicles purified from L. plantarum BGAN8 cell-free supernatants. ( A ) EVs visualized by <t>cryo-transmission</t> electron microscopy (bar 200 nm); size of the particles (from up to bottom and left to right): 115.2 nm, 69.5 nm, 91.3 nm and 40.6 nm). ( B ) Size distribution of the EVs according to the diameter range (nm) measured under the <t>cryo-TEM</t> microscope.
    The new Thermo Scientific Krios G4 Cryo Transmission Electron Microscope Cryo TEM enables you to unravel life at the molecular level easier faster and more reliably than ever before The most compact TEM in its class the Krios G4 Cryo TEM consists of a highly stable 300 kV TEM platform and the industry leading Autoloader cryogenic sample manipulation robot making it ideally suited for automated applications such as single particle analysis SPA cryo electron tomography cryoET and micro electron diffraction MicroED Through a thorough redesign of the mechanical base frame and system enclosure the microscope height has been reduced to below 3 meters which allows for instrument installation in labs with a ceiling height below 3 04m 10 ft thereby avoiding costly room renovations Designed in connectivity ensures a robust and risk free pathway throughout the entire workflow from sample preparation and optimization to image acquisition and data processing
    https://www.bioz.com/result/cryo tem microscope/product/Thermo Fisher
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    cryo tem microscope - by Bioz Stars, 2021-09
    99/100 stars

    Images

    1) Product Images from "Proteomic profile of extracellular vesicles released by Lactiplantibacillus plantarum BGAN8 and their internalization by non-polarized HT29 cell line"

    Article Title: Proteomic profile of extracellular vesicles released by Lactiplantibacillus plantarum BGAN8 and their internalization by non-polarized HT29 cell line

    Journal: Scientific Reports

    doi: 10.1038/s41598-020-78920-z

    Extracellular vesicles purified from L. plantarum BGAN8 cell-free supernatants. ( A ) EVs visualized by cryo-transmission electron microscopy (bar 200 nm); size of the particles (from up to bottom and left to right): 115.2 nm, 69.5 nm, 91.3 nm and 40.6 nm). ( B ) Size distribution of the EVs according to the diameter range (nm) measured under the cryo-TEM microscope.
    Figure Legend Snippet: Extracellular vesicles purified from L. plantarum BGAN8 cell-free supernatants. ( A ) EVs visualized by cryo-transmission electron microscopy (bar 200 nm); size of the particles (from up to bottom and left to right): 115.2 nm, 69.5 nm, 91.3 nm and 40.6 nm). ( B ) Size distribution of the EVs according to the diameter range (nm) measured under the cryo-TEM microscope.

    Techniques Used: Purification, Transmission Assay, Electron Microscopy, Transmission Electron Microscopy, Microscopy

    2) Product Images from "Intestinal epithelial NAIP/NLRC4 restricts systemic dissemination of the adapted pathogen Salmonella Typhimurium due to site-specific bacterial PAMP expression"

    Article Title: Intestinal epithelial NAIP/NLRC4 restricts systemic dissemination of the adapted pathogen Salmonella Typhimurium due to site-specific bacterial PAMP expression

    Journal: Mucosal Immunology

    doi: 10.1038/s41385-019-0247-0

    S . Tm expresses PAMPs recognized by NAIP/NLRC4 in the cecal epithelium, but downregulates them upon traversal. a S . Tm with potentially detached (left/upper panel) and attached (right/lower panel) flagella can be found inside SCVs. Shown are 15 nm slices of cryotomograms of cryo-FIB milled HeLa cells and the respective segmentations (representative for six tomograms; frozen at 1 hpi and MOI 300). OM: outer membrane. IM: inner membrane. SCV: Salmonella containing vacuole. hCP: host cell cytoplasm. Scale bar, 100 nm. b S . Tm as a rule expresses SPI-1 in the cecum (12 hpi, n total S . Tm =322) and downregulates the expression in the spleen (3 dpi, n total S . Tm =35) of JH −/− mice. c The fraction of SPI-1 + S . Tm within the cecal mucosa decreases upon traversal to the lamina propria (n total S . Tm epithelium =253; n total S . Tm lamina propria =69). Quantifications shown in b and c are based on fluorescence microscopy of S . Tm SPI-1-GFP expressing cytoplasmic GFP under the prgH -promotor and an LPS immunostaining. d Representative images of tissue sections (cecum, spleen), which were used for the quantifications in b and c . e Flagella are prominent in the cecal mucosa (12 hpi, n total S . Tm =341), while they are completely absent in the spleen (3 dpi, n total S . Tm =50) of JH −/− mice. Quantifications were performed by fluorescence immunostaining of FliC. f Similar to SPI-1 ( c ) expression, flagella are less evident in the lamina propria (n total S . Tm lamina propria =39) than within IECs (n total S . Tm epithelium =302). g Representative images of tissue sections (cecum, spleen), which were used for the quantifications in e and f . White arrowheads: flagella; white arrows: SPI-1-GFP + S . Tm (epithelium and lamina propria); E: epithelium; L: lumen; LP: lamina propria. Black bar: Median. Statistical analysis: Mann-Whitney- U Test, p -values indicated. Scale bars, 10 µm.
    Figure Legend Snippet: S . Tm expresses PAMPs recognized by NAIP/NLRC4 in the cecal epithelium, but downregulates them upon traversal. a S . Tm with potentially detached (left/upper panel) and attached (right/lower panel) flagella can be found inside SCVs. Shown are 15 nm slices of cryotomograms of cryo-FIB milled HeLa cells and the respective segmentations (representative for six tomograms; frozen at 1 hpi and MOI 300). OM: outer membrane. IM: inner membrane. SCV: Salmonella containing vacuole. hCP: host cell cytoplasm. Scale bar, 100 nm. b S . Tm as a rule expresses SPI-1 in the cecum (12 hpi, n total S . Tm =322) and downregulates the expression in the spleen (3 dpi, n total S . Tm =35) of JH −/− mice. c The fraction of SPI-1 + S . Tm within the cecal mucosa decreases upon traversal to the lamina propria (n total S . Tm epithelium =253; n total S . Tm lamina propria =69). Quantifications shown in b and c are based on fluorescence microscopy of S . Tm SPI-1-GFP expressing cytoplasmic GFP under the prgH -promotor and an LPS immunostaining. d Representative images of tissue sections (cecum, spleen), which were used for the quantifications in b and c . e Flagella are prominent in the cecal mucosa (12 hpi, n total S . Tm =341), while they are completely absent in the spleen (3 dpi, n total S . Tm =50) of JH −/− mice. Quantifications were performed by fluorescence immunostaining of FliC. f Similar to SPI-1 ( c ) expression, flagella are less evident in the lamina propria (n total S . Tm lamina propria =39) than within IECs (n total S . Tm epithelium =302). g Representative images of tissue sections (cecum, spleen), which were used for the quantifications in e and f . White arrowheads: flagella; white arrows: SPI-1-GFP + S . Tm (epithelium and lamina propria); E: epithelium; L: lumen; LP: lamina propria. Black bar: Median. Statistical analysis: Mann-Whitney- U Test, p -values indicated. Scale bars, 10 µm.

    Techniques Used: Expressing, Mouse Assay, Fluorescence, Microscopy, Immunostaining, MANN-WHITNEY

    3) Product Images from "Demonstration of electron diffraction from membrane protein crystals grown in a lipidic mesophase after lamella preparation by focused ion beam milling at cryogenic temperatures"

    Article Title: Demonstration of electron diffraction from membrane protein crystals grown in a lipidic mesophase after lamella preparation by focused ion beam milling at cryogenic temperatures

    Journal: bioRxiv

    doi: 10.1101/2020.07.03.186049

    Electron diffraction experiment on a 210 nm thick lamella of bacteriorhodopsin, using a 200-kV cryo-TEM microscope. (a) TEM micrograph of the FIB-machined lamella of the BR crystal. Electron diffraction signal was collected from a 1.4-µm area of the lamella, indicated by a red circle with a cross. The scale bar on (a) corresponds to 5 µm. (b) The 200-kV electron diffraction pattern obtained from the area indicated in (a). (c) The electron diffraction image is corrected by subtraction of local moving-average background, calculated with the Adxv program ( Adxv, 2013 ). The inset shows a close up of the electron diffraction pattern. (d) Diffraction peaks were automatically picked up by the Adxv software, and the diffraction peaks in the resolution shell of 2.7 Å - 2.45 Å had a mean signal-to-noise ratio
    Figure Legend Snippet: Electron diffraction experiment on a 210 nm thick lamella of bacteriorhodopsin, using a 200-kV cryo-TEM microscope. (a) TEM micrograph of the FIB-machined lamella of the BR crystal. Electron diffraction signal was collected from a 1.4-µm area of the lamella, indicated by a red circle with a cross. The scale bar on (a) corresponds to 5 µm. (b) The 200-kV electron diffraction pattern obtained from the area indicated in (a). (c) The electron diffraction image is corrected by subtraction of local moving-average background, calculated with the Adxv program ( Adxv, 2013 ). The inset shows a close up of the electron diffraction pattern. (d) Diffraction peaks were automatically picked up by the Adxv software, and the diffraction peaks in the resolution shell of 2.7 Å - 2.45 Å had a mean signal-to-noise ratio

    Techniques Used: Transmission Electron Microscopy, Microscopy, Software

    Related Articles

    Tomography:

    Article Title: Structural Basis and Mode of Action for Two Broadly Neutralizing Antibodies Against SARS-CoV-2 Emerging Variants of Concern
    Article Snippet: .. Cryo-electron Tomography Data Collection Cryo-grids were imaged on a cryo-transmission electron microscope (Titan Krios, Thermo Fisher Scientific) operated at 300 kV, using a Gatan K3 direct electron detector in counting mode with a 20 eV energy slit. ..

    Microscopy:

    Article Title: Structural Basis and Mode of Action for Two Broadly Neutralizing Antibodies Against SARS-CoV-2 Emerging Variants of Concern
    Article Snippet: .. Cryo-electron Tomography Data Collection Cryo-grids were imaged on a cryo-transmission electron microscope (Titan Krios, Thermo Fisher Scientific) operated at 300 kV, using a Gatan K3 direct electron detector in counting mode with a 20 eV energy slit. ..

    Article Title: Proteomic profile of extracellular vesicles released by Lactiplantibacillus plantarum BGAN8 and their internalization by non-polarized HT29 cell line
    Article Snippet: .. The vitrified sample was stored in liquid nitrogen until its observation in the cryo-TEM microscope. ..

    Article Title: Molecular plasticity of the native mouse skeletal sarcomere revealed by cryo-ET
    Article Snippet: .. Electron cryo-tomography and tomogram reconstruction Autogrids were rotated by 90° after milling before being inserted into an autogrid cassette so as to align the longitudinal axis of lamellae perpendicular to the tilt axis of the transmission electron microscope (TEM) stage later. ..

    other:

    Article Title: Real-Time Conformational Dynamics of SARS-CoV-2 Spikes on Virus Particles
    Article Snippet: Cryo-electron Tomography 6 nm gold tracer was added to the concentrated S-decorated HIV-1 lentivirus and S-MEN particles viruses at 1:3 ratio, and 5 μl of the mixture was placed onto freshly glow discharged holey carbon grids for 1 min. Grids were blotted with filter paper, and rapidly frozen in liquid ethane using a homemade gravity-driven plunger apparatus.

    Article Title: The SARS-CoV-2 nucleocapsid phosphoprotein forms mutually exclusive condensates with RNA and the membrane-associated M protein
    Article Snippet: Cryo-electron tomography For cryo-ET, phase separation was induced by mixing 10 µM N protein (in 5 mM HEPES, pH 7.5, 80 mM KCl) with 20 ng/µL of the indicated RNA (in 5 mM HEPES, pH 7.5) for 0.5–1 min at 20 °C.

    Article Title: A CRISPR interference platform for selective downregulation of gene expression in Borrelia burgdorferi
    Article Snippet: Cryo-electron tomography and three-dimensional visualization B. burgdorferi cultures growing exponentially in BSK-II medium to no more that 5×107 cells/mL were pelleted for 10 minutes at 3,000 x g at room temperature.

    Article Title: Quality Assessment and Comparison of Plasma-Derived Extracellular Vesicles Separated by Three Commercial Kits for Prostate Cancer Diagnosis
    Article Snippet: The vitrified samples were stored in liquid nitrogen (−196 °C) prior to cryo-TEM analysis.

    Transmission Assay:

    Article Title: Molecular plasticity of the native mouse skeletal sarcomere revealed by cryo-ET
    Article Snippet: .. Electron cryo-tomography and tomogram reconstruction Autogrids were rotated by 90° after milling before being inserted into an autogrid cassette so as to align the longitudinal axis of lamellae perpendicular to the tilt axis of the transmission electron microscope (TEM) stage later. ..

    Transmission Electron Microscopy:

    Article Title: Molecular plasticity of the native mouse skeletal sarcomere revealed by cryo-ET
    Article Snippet: .. Electron cryo-tomography and tomogram reconstruction Autogrids were rotated by 90° after milling before being inserted into an autogrid cassette so as to align the longitudinal axis of lamellae perpendicular to the tilt axis of the transmission electron microscope (TEM) stage later. ..

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  • 86
    Thermo Fisher cryo tem
    <t>Cryo-TEM</t> micrographs of the WLMs with different FE-NP concentrations: ( a ) 0 wt%; ( b ) 0.1 wt%; ( c ) 0.3 wt%; and ( d ) 0.5 wt%.
    Cryo Tem, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/cryo tem/product/Thermo Fisher
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    cryo tem - by Bioz Stars, 2021-09
    86/100 stars
      Buy from Supplier

    99
    Thermo Fisher cryo tem microscope
    Extracellular vesicles purified from L. plantarum BGAN8 cell-free supernatants. ( A ) EVs visualized by <t>cryo-transmission</t> electron microscopy (bar 200 nm); size of the particles (from up to bottom and left to right): 115.2 nm, 69.5 nm, 91.3 nm and 40.6 nm). ( B ) Size distribution of the EVs according to the diameter range (nm) measured under the <t>cryo-TEM</t> microscope.
    Cryo Tem Microscope, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/cryo tem microscope/product/Thermo Fisher
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    cryo tem microscope - by Bioz Stars, 2021-09
    99/100 stars
      Buy from Supplier

    Image Search Results


    Cryo-TEM micrographs of the WLMs with different FE-NP concentrations: ( a ) 0 wt%; ( b ) 0.1 wt%; ( c ) 0.3 wt%; and ( d ) 0.5 wt%.

    Journal: Nanomaterials

    Article Title: Experimental Study on the Drag Reduction Performance of Clear Fracturing Fluid Using Wormlike Surfactant Micelles and Magnetic Nanoparticles under a Magnetic Field

    doi: 10.3390/nano11040885

    Figure Lengend Snippet: Cryo-TEM micrographs of the WLMs with different FE-NP concentrations: ( a ) 0 wt%; ( b ) 0.1 wt%; ( c ) 0.3 wt%; and ( d ) 0.5 wt%.

    Article Snippet: Then, the frozen specimen was transferred into a cryo-holder and was observed with cryo-TEM (Krios G4, Thermo Fisher Scientific Inc., Waltham, MA, USA).

    Techniques: Transmission Electron Microscopy

    Extracellular vesicles purified from L. plantarum BGAN8 cell-free supernatants. ( A ) EVs visualized by cryo-transmission electron microscopy (bar 200 nm); size of the particles (from up to bottom and left to right): 115.2 nm, 69.5 nm, 91.3 nm and 40.6 nm). ( B ) Size distribution of the EVs according to the diameter range (nm) measured under the cryo-TEM microscope.

    Journal: Scientific Reports

    Article Title: Proteomic profile of extracellular vesicles released by Lactiplantibacillus plantarum BGAN8 and their internalization by non-polarized HT29 cell line

    doi: 10.1038/s41598-020-78920-z

    Figure Lengend Snippet: Extracellular vesicles purified from L. plantarum BGAN8 cell-free supernatants. ( A ) EVs visualized by cryo-transmission electron microscopy (bar 200 nm); size of the particles (from up to bottom and left to right): 115.2 nm, 69.5 nm, 91.3 nm and 40.6 nm). ( B ) Size distribution of the EVs according to the diameter range (nm) measured under the cryo-TEM microscope.

    Article Snippet: The vitrified sample was stored in liquid nitrogen until its observation in the cryo-TEM microscope.

    Techniques: Purification, Transmission Assay, Electron Microscopy, Transmission Electron Microscopy, Microscopy

    S . Tm expresses PAMPs recognized by NAIP/NLRC4 in the cecal epithelium, but downregulates them upon traversal. a S . Tm with potentially detached (left/upper panel) and attached (right/lower panel) flagella can be found inside SCVs. Shown are 15 nm slices of cryotomograms of cryo-FIB milled HeLa cells and the respective segmentations (representative for six tomograms; frozen at 1 hpi and MOI 300). OM: outer membrane. IM: inner membrane. SCV: Salmonella containing vacuole. hCP: host cell cytoplasm. Scale bar, 100 nm. b S . Tm as a rule expresses SPI-1 in the cecum (12 hpi, n total S . Tm =322) and downregulates the expression in the spleen (3 dpi, n total S . Tm =35) of JH −/− mice. c The fraction of SPI-1 + S . Tm within the cecal mucosa decreases upon traversal to the lamina propria (n total S . Tm epithelium =253; n total S . Tm lamina propria =69). Quantifications shown in b and c are based on fluorescence microscopy of S . Tm SPI-1-GFP expressing cytoplasmic GFP under the prgH -promotor and an LPS immunostaining. d Representative images of tissue sections (cecum, spleen), which were used for the quantifications in b and c . e Flagella are prominent in the cecal mucosa (12 hpi, n total S . Tm =341), while they are completely absent in the spleen (3 dpi, n total S . Tm =50) of JH −/− mice. Quantifications were performed by fluorescence immunostaining of FliC. f Similar to SPI-1 ( c ) expression, flagella are less evident in the lamina propria (n total S . Tm lamina propria =39) than within IECs (n total S . Tm epithelium =302). g Representative images of tissue sections (cecum, spleen), which were used for the quantifications in e and f . White arrowheads: flagella; white arrows: SPI-1-GFP + S . Tm (epithelium and lamina propria); E: epithelium; L: lumen; LP: lamina propria. Black bar: Median. Statistical analysis: Mann-Whitney- U Test, p -values indicated. Scale bars, 10 µm.

    Journal: Mucosal Immunology

    Article Title: Intestinal epithelial NAIP/NLRC4 restricts systemic dissemination of the adapted pathogen Salmonella Typhimurium due to site-specific bacterial PAMP expression

    doi: 10.1038/s41385-019-0247-0

    Figure Lengend Snippet: S . Tm expresses PAMPs recognized by NAIP/NLRC4 in the cecal epithelium, but downregulates them upon traversal. a S . Tm with potentially detached (left/upper panel) and attached (right/lower panel) flagella can be found inside SCVs. Shown are 15 nm slices of cryotomograms of cryo-FIB milled HeLa cells and the respective segmentations (representative for six tomograms; frozen at 1 hpi and MOI 300). OM: outer membrane. IM: inner membrane. SCV: Salmonella containing vacuole. hCP: host cell cytoplasm. Scale bar, 100 nm. b S . Tm as a rule expresses SPI-1 in the cecum (12 hpi, n total S . Tm =322) and downregulates the expression in the spleen (3 dpi, n total S . Tm =35) of JH −/− mice. c The fraction of SPI-1 + S . Tm within the cecal mucosa decreases upon traversal to the lamina propria (n total S . Tm epithelium =253; n total S . Tm lamina propria =69). Quantifications shown in b and c are based on fluorescence microscopy of S . Tm SPI-1-GFP expressing cytoplasmic GFP under the prgH -promotor and an LPS immunostaining. d Representative images of tissue sections (cecum, spleen), which were used for the quantifications in b and c . e Flagella are prominent in the cecal mucosa (12 hpi, n total S . Tm =341), while they are completely absent in the spleen (3 dpi, n total S . Tm =50) of JH −/− mice. Quantifications were performed by fluorescence immunostaining of FliC. f Similar to SPI-1 ( c ) expression, flagella are less evident in the lamina propria (n total S . Tm lamina propria =39) than within IECs (n total S . Tm epithelium =302). g Representative images of tissue sections (cecum, spleen), which were used for the quantifications in e and f . White arrowheads: flagella; white arrows: SPI-1-GFP + S . Tm (epithelium and lamina propria); E: epithelium; L: lumen; LP: lamina propria. Black bar: Median. Statistical analysis: Mann-Whitney- U Test, p -values indicated. Scale bars, 10 µm.

    Article Snippet: Cryo-focused ion beam milling Cryo-focused ion beam (cryoFIB) milling was used to prepare samples of plunge-frozen infected HeLa cells that could then be imaged by cryo-electron tomography.

    Techniques: Expressing, Mouse Assay, Fluorescence, Microscopy, Immunostaining, MANN-WHITNEY

    Electron diffraction experiment on a 210 nm thick lamella of bacteriorhodopsin, using a 200-kV cryo-TEM microscope. (a) TEM micrograph of the FIB-machined lamella of the BR crystal. Electron diffraction signal was collected from a 1.4-µm area of the lamella, indicated by a red circle with a cross. The scale bar on (a) corresponds to 5 µm. (b) The 200-kV electron diffraction pattern obtained from the area indicated in (a). (c) The electron diffraction image is corrected by subtraction of local moving-average background, calculated with the Adxv program ( Adxv, 2013 ). The inset shows a close up of the electron diffraction pattern. (d) Diffraction peaks were automatically picked up by the Adxv software, and the diffraction peaks in the resolution shell of 2.7 Å - 2.45 Å had a mean signal-to-noise ratio

    Journal: bioRxiv

    Article Title: Demonstration of electron diffraction from membrane protein crystals grown in a lipidic mesophase after lamella preparation by focused ion beam milling at cryogenic temperatures

    doi: 10.1101/2020.07.03.186049

    Figure Lengend Snippet: Electron diffraction experiment on a 210 nm thick lamella of bacteriorhodopsin, using a 200-kV cryo-TEM microscope. (a) TEM micrograph of the FIB-machined lamella of the BR crystal. Electron diffraction signal was collected from a 1.4-µm area of the lamella, indicated by a red circle with a cross. The scale bar on (a) corresponds to 5 µm. (b) The 200-kV electron diffraction pattern obtained from the area indicated in (a). (c) The electron diffraction image is corrected by subtraction of local moving-average background, calculated with the Adxv program ( Adxv, 2013 ). The inset shows a close up of the electron diffraction pattern. (d) Diffraction peaks were automatically picked up by the Adxv software, and the diffraction peaks in the resolution shell of 2.7 Å - 2.45 Å had a mean signal-to-noise ratio

    Article Snippet: This implies that, in order to collect ED data suitable for high-resolution structural determination with the experimental system used in the present article, at least 3-6 different lamellae have to be produced and have to survive transfer from the cryo-FIB instrument to the cryo-TEM microscope.

    Techniques: Transmission Electron Microscopy, Microscopy, Software