Journal: Materials Today Bio
Article Title: The involvement of extracellular vesicles in the transcytosis of nanoliposomes through brain endothelial cells, and the impact of liposomal pH-sensitivity
doi: 10.1016/j.mtbio.2022.100212
Figure Lengend Snippet: Physicochemical properties of isolated vesicles from hBMECs following treatment with GSH-PEG-L and GSH-PEG-pSL for 2 h versus EVs from non-treated cells (control). A) the particle numbers in the sEV and mEV fractions; B C) the FIs of Rh-PE (B) and calcein (C) in the EV lysates; D) Vesicle size, zeta potential and morphology under cryo-TEM. Vesicles secreted from cells were fractioned at various centrifugation speeds of 2000 g– defined as mEV 2000 g fraction, 10000 g – mEVs and 100,000 g – sEVs.
Article Snippet: The morphology and lamellar structure of frozen samples of the isolated vesicles was viewed with Cryo-TEM (Tecnai 12 electron microscope, FEI, Hillsboro, USA) as previously described [ , ].
Techniques: Isolation, Transmission Electron Microscopy, Centrifugation