kt5823  (Alomone Labs)


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    Structured Review

    Alomone Labs kt5823
    Inhibition of PKG prevented the proliferation of SVZ cells stimulated by PDE5 inhibitors. Cell proliferation following treatment with 1 μ M <t>KT5823</t> and 1 μ M T0156 (a, d), 1 μ M sildenafil (b, e), or 10 μ M zaprinast (c, f) was assessed by incorporation of EdU and evaluated by flow cytometry, following 6 h or 24 h of treatment. Data are expressed as means ± SEM of at least 4 independent experiments. One-way ANOVA (Bonferroni's post-test), * P
    Kt5823, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 85/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/kt5823/product/Alomone Labs
    Average 85 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    kt5823 - by Bioz Stars, 2022-08
    85/100 stars

    Images

    1) Product Images from "Stimulation of Neural Stem Cell Proliferation by Inhibition of Phosphodiesterase 5"

    Article Title: Stimulation of Neural Stem Cell Proliferation by Inhibition of Phosphodiesterase 5

    Journal: Stem Cells International

    doi: 10.1155/2014/878397

    Inhibition of PKG prevented the proliferation of SVZ cells stimulated by PDE5 inhibitors. Cell proliferation following treatment with 1 μ M KT5823 and 1 μ M T0156 (a, d), 1 μ M sildenafil (b, e), or 10 μ M zaprinast (c, f) was assessed by incorporation of EdU and evaluated by flow cytometry, following 6 h or 24 h of treatment. Data are expressed as means ± SEM of at least 4 independent experiments. One-way ANOVA (Bonferroni's post-test), * P
    Figure Legend Snippet: Inhibition of PKG prevented the proliferation of SVZ cells stimulated by PDE5 inhibitors. Cell proliferation following treatment with 1 μ M KT5823 and 1 μ M T0156 (a, d), 1 μ M sildenafil (b, e), or 10 μ M zaprinast (c, f) was assessed by incorporation of EdU and evaluated by flow cytometry, following 6 h or 24 h of treatment. Data are expressed as means ± SEM of at least 4 independent experiments. One-way ANOVA (Bonferroni's post-test), * P

    Techniques Used: Inhibition, Flow Cytometry, Cytometry

    Activation of sGC stimulates proliferation of NSC, increases ERK1/2 phosphorylation, and decreases p27 Kip1 levels. Cell proliferation following treatment with 20 μ M YC-1 (a) for 24 h was assessed by the incorporation of EdU and analyzed by flow cytometry. Levels of phospho-ERK1/2 following treatment with 20 μ M YC-1 (b) or YC-1 plus 1 μ M KT5823 (c) and p27 Kip1 levels following treatment with YC-1 (d) or YC-1 plus 1 μ M U0126 (e) or KT5823 (f) for 2 h were assessed by Western blot. Representative images are shown. Data are expressed as means ± SEM of at least 4 independent experiments. (a), (b), and (d) two-tailed t -test, * P
    Figure Legend Snippet: Activation of sGC stimulates proliferation of NSC, increases ERK1/2 phosphorylation, and decreases p27 Kip1 levels. Cell proliferation following treatment with 20 μ M YC-1 (a) for 24 h was assessed by the incorporation of EdU and analyzed by flow cytometry. Levels of phospho-ERK1/2 following treatment with 20 μ M YC-1 (b) or YC-1 plus 1 μ M KT5823 (c) and p27 Kip1 levels following treatment with YC-1 (d) or YC-1 plus 1 μ M U0126 (e) or KT5823 (f) for 2 h were assessed by Western blot. Representative images are shown. Data are expressed as means ± SEM of at least 4 independent experiments. (a), (b), and (d) two-tailed t -test, * P

    Techniques Used: Activation Assay, Flow Cytometry, Cytometry, Western Blot, Two Tailed Test

    Inhibition of PKG did not prevent the decrease of p27 Kip1 levels by T0156. p27 Kip1 levels following treatment with 1 μ M KT5823 and 1 μ M T0156 (a), 1 μ M sildenafil (b), or 10 μ M zaprinast (c) for 2 h were assessed by Western blot. Representative images are shown. Data are expressed as means ± SEM of at least 4 independent experiments. One-way ANOVA (Bonferroni's post-test), * P
    Figure Legend Snippet: Inhibition of PKG did not prevent the decrease of p27 Kip1 levels by T0156. p27 Kip1 levels following treatment with 1 μ M KT5823 and 1 μ M T0156 (a), 1 μ M sildenafil (b), or 10 μ M zaprinast (c) for 2 h were assessed by Western blot. Representative images are shown. Data are expressed as means ± SEM of at least 4 independent experiments. One-way ANOVA (Bonferroni's post-test), * P

    Techniques Used: Inhibition, Western Blot

    Inhibition of PKG prevented the phosphorylation of ERK1/2 by treatment with T0156 or zaprinast. Levels of phospho-ERK1/2 following treatment with 1 μ M KT5823 and 1 μ M T0156 (a), 1 μ M sildenafil (b), and 10 μ M zaprinast (c) for 2 h were assessed by Western blot. Representative images are shown. Data are expressed as means ± SEM of at least 4 independent experiments. One-way ANOVA (Bonferroni's post-test), * P
    Figure Legend Snippet: Inhibition of PKG prevented the phosphorylation of ERK1/2 by treatment with T0156 or zaprinast. Levels of phospho-ERK1/2 following treatment with 1 μ M KT5823 and 1 μ M T0156 (a), 1 μ M sildenafil (b), and 10 μ M zaprinast (c) for 2 h were assessed by Western blot. Representative images are shown. Data are expressed as means ± SEM of at least 4 independent experiments. One-way ANOVA (Bonferroni's post-test), * P

    Techniques Used: Inhibition, Western Blot

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    Alomone Labs kt5823
    Inhibition of PKG prevented the proliferation of SVZ cells stimulated by PDE5 inhibitors. Cell proliferation following treatment with 1 μ M <t>KT5823</t> and 1 μ M T0156 (a, d), 1 μ M sildenafil (b, e), or 10 μ M zaprinast (c, f) was assessed by incorporation of EdU and evaluated by flow cytometry, following 6 h or 24 h of treatment. Data are expressed as means ± SEM of at least 4 independent experiments. One-way ANOVA (Bonferroni's post-test), * P
    Kt5823, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/kt5823/product/Alomone Labs
    Average 85 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    kt5823 - by Bioz Stars, 2022-08
    85/100 stars
      Buy from Supplier

    93
    Alomone Labs k252a
    A–C . Conventional protein kinase C isoforms (PKCα,β,γ) effectively phosphorylate p75NTR and this phosphorylation is abolished by the kinase inhibitor <t>K252a.</t> A . Human p75NTR is phosphorylated in vitro by several kinases,
    K252a, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/k252a/product/Alomone Labs
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    k252a - by Bioz Stars, 2022-08
    93/100 stars
      Buy from Supplier

    Image Search Results


    Inhibition of PKG prevented the proliferation of SVZ cells stimulated by PDE5 inhibitors. Cell proliferation following treatment with 1 μ M KT5823 and 1 μ M T0156 (a, d), 1 μ M sildenafil (b, e), or 10 μ M zaprinast (c, f) was assessed by incorporation of EdU and evaluated by flow cytometry, following 6 h or 24 h of treatment. Data are expressed as means ± SEM of at least 4 independent experiments. One-way ANOVA (Bonferroni's post-test), * P

    Journal: Stem Cells International

    Article Title: Stimulation of Neural Stem Cell Proliferation by Inhibition of Phosphodiesterase 5

    doi: 10.1155/2014/878397

    Figure Lengend Snippet: Inhibition of PKG prevented the proliferation of SVZ cells stimulated by PDE5 inhibitors. Cell proliferation following treatment with 1 μ M KT5823 and 1 μ M T0156 (a, d), 1 μ M sildenafil (b, e), or 10 μ M zaprinast (c, f) was assessed by incorporation of EdU and evaluated by flow cytometry, following 6 h or 24 h of treatment. Data are expressed as means ± SEM of at least 4 independent experiments. One-way ANOVA (Bonferroni's post-test), * P

    Article Snippet: KT5823 was obtained from Alomone Labs (Jerusalem, Israel) and 3-(5′-Hydroxymethyl-2′-furyl)-1-benzylindazole (YC-1) from Santa Cruz Biotechnology (Santa Cruz, CA, USA).

    Techniques: Inhibition, Flow Cytometry, Cytometry

    Activation of sGC stimulates proliferation of NSC, increases ERK1/2 phosphorylation, and decreases p27 Kip1 levels. Cell proliferation following treatment with 20 μ M YC-1 (a) for 24 h was assessed by the incorporation of EdU and analyzed by flow cytometry. Levels of phospho-ERK1/2 following treatment with 20 μ M YC-1 (b) or YC-1 plus 1 μ M KT5823 (c) and p27 Kip1 levels following treatment with YC-1 (d) or YC-1 plus 1 μ M U0126 (e) or KT5823 (f) for 2 h were assessed by Western blot. Representative images are shown. Data are expressed as means ± SEM of at least 4 independent experiments. (a), (b), and (d) two-tailed t -test, * P

    Journal: Stem Cells International

    Article Title: Stimulation of Neural Stem Cell Proliferation by Inhibition of Phosphodiesterase 5

    doi: 10.1155/2014/878397

    Figure Lengend Snippet: Activation of sGC stimulates proliferation of NSC, increases ERK1/2 phosphorylation, and decreases p27 Kip1 levels. Cell proliferation following treatment with 20 μ M YC-1 (a) for 24 h was assessed by the incorporation of EdU and analyzed by flow cytometry. Levels of phospho-ERK1/2 following treatment with 20 μ M YC-1 (b) or YC-1 plus 1 μ M KT5823 (c) and p27 Kip1 levels following treatment with YC-1 (d) or YC-1 plus 1 μ M U0126 (e) or KT5823 (f) for 2 h were assessed by Western blot. Representative images are shown. Data are expressed as means ± SEM of at least 4 independent experiments. (a), (b), and (d) two-tailed t -test, * P

    Article Snippet: KT5823 was obtained from Alomone Labs (Jerusalem, Israel) and 3-(5′-Hydroxymethyl-2′-furyl)-1-benzylindazole (YC-1) from Santa Cruz Biotechnology (Santa Cruz, CA, USA).

    Techniques: Activation Assay, Flow Cytometry, Cytometry, Western Blot, Two Tailed Test

    Inhibition of PKG did not prevent the decrease of p27 Kip1 levels by T0156. p27 Kip1 levels following treatment with 1 μ M KT5823 and 1 μ M T0156 (a), 1 μ M sildenafil (b), or 10 μ M zaprinast (c) for 2 h were assessed by Western blot. Representative images are shown. Data are expressed as means ± SEM of at least 4 independent experiments. One-way ANOVA (Bonferroni's post-test), * P

    Journal: Stem Cells International

    Article Title: Stimulation of Neural Stem Cell Proliferation by Inhibition of Phosphodiesterase 5

    doi: 10.1155/2014/878397

    Figure Lengend Snippet: Inhibition of PKG did not prevent the decrease of p27 Kip1 levels by T0156. p27 Kip1 levels following treatment with 1 μ M KT5823 and 1 μ M T0156 (a), 1 μ M sildenafil (b), or 10 μ M zaprinast (c) for 2 h were assessed by Western blot. Representative images are shown. Data are expressed as means ± SEM of at least 4 independent experiments. One-way ANOVA (Bonferroni's post-test), * P

    Article Snippet: KT5823 was obtained from Alomone Labs (Jerusalem, Israel) and 3-(5′-Hydroxymethyl-2′-furyl)-1-benzylindazole (YC-1) from Santa Cruz Biotechnology (Santa Cruz, CA, USA).

    Techniques: Inhibition, Western Blot

    Inhibition of PKG prevented the phosphorylation of ERK1/2 by treatment with T0156 or zaprinast. Levels of phospho-ERK1/2 following treatment with 1 μ M KT5823 and 1 μ M T0156 (a), 1 μ M sildenafil (b), and 10 μ M zaprinast (c) for 2 h were assessed by Western blot. Representative images are shown. Data are expressed as means ± SEM of at least 4 independent experiments. One-way ANOVA (Bonferroni's post-test), * P

    Journal: Stem Cells International

    Article Title: Stimulation of Neural Stem Cell Proliferation by Inhibition of Phosphodiesterase 5

    doi: 10.1155/2014/878397

    Figure Lengend Snippet: Inhibition of PKG prevented the phosphorylation of ERK1/2 by treatment with T0156 or zaprinast. Levels of phospho-ERK1/2 following treatment with 1 μ M KT5823 and 1 μ M T0156 (a), 1 μ M sildenafil (b), and 10 μ M zaprinast (c) for 2 h were assessed by Western blot. Representative images are shown. Data are expressed as means ± SEM of at least 4 independent experiments. One-way ANOVA (Bonferroni's post-test), * P

    Article Snippet: KT5823 was obtained from Alomone Labs (Jerusalem, Israel) and 3-(5′-Hydroxymethyl-2′-furyl)-1-benzylindazole (YC-1) from Santa Cruz Biotechnology (Santa Cruz, CA, USA).

    Techniques: Inhibition, Western Blot

    Effects of BPA on [Ca 2+ ] i oscillations through a PKG-mediated mechanism. ( A ) Low-glucose–induced [Ca 2+ ] i oscillations blocked by 1 nM BPA. ( B ) Frequency of [Ca 2+ ] i oscillations were not reduced by BPA in an islet from the same preparation and maintained in the same conditions but pretreated with and exposed to the PKG inhibitor KT-5823 (1 μM). ( C ) Mean frequency values of 0.5 mM glucose before application of either BPA (G1) or E 2 (G2), in the presence of 1 nM BPA or 1 nM E 2 , as in ( A ) , or in the presence of 1 μM KT-5823 plus 0.5 mM glucose (KT); KT plus 1 nM BPA (BPA + KT), and KT plus 1 nM 17β-E 2 (E 2 + KT) as in ( B ). Results are representative of at least 12 cells from nine different islets, expressed as mean ± SE. * p

    Journal: Environmental Health Perspectives

    Article Title: Low Doses of Bisphenol A and Diethylstilbestrol Impair Ca2+ Signals in Pancreatic ?-Cells through a Nonclassical Membrane Estrogen Receptor within Intact Islets of Langerhans

    doi: 10.1289/ehp.8002

    Figure Lengend Snippet: Effects of BPA on [Ca 2+ ] i oscillations through a PKG-mediated mechanism. ( A ) Low-glucose–induced [Ca 2+ ] i oscillations blocked by 1 nM BPA. ( B ) Frequency of [Ca 2+ ] i oscillations were not reduced by BPA in an islet from the same preparation and maintained in the same conditions but pretreated with and exposed to the PKG inhibitor KT-5823 (1 μM). ( C ) Mean frequency values of 0.5 mM glucose before application of either BPA (G1) or E 2 (G2), in the presence of 1 nM BPA or 1 nM E 2 , as in ( A ) , or in the presence of 1 μM KT-5823 plus 0.5 mM glucose (KT); KT plus 1 nM BPA (BPA + KT), and KT plus 1 nM 17β-E 2 (E 2 + KT) as in ( B ). Results are representative of at least 12 cells from nine different islets, expressed as mean ± SE. * p

    Article Snippet: Thus, KT-5823 does not alter [Ca2+ ]i oscillations but prevents the suppression of low-glucose–induced [Ca2+ ]i oscillations by BPA, indicating that BPA’s effect is exerted by a cGMP/PKG-mediated mechanism, as has been demonstrated for the natural hormone 17β-E2 ( ).

    Techniques:

    Effects of 8Br-cGMP on EDCs and E 2 via PKG. ( A ) Exposure to 10 μM 8Br-cGMP dramatically reduces the frequency of low-glucose–induced [Ca 2+ ] i oscillations. ( B ) After incubation with the specific PKG inhibitor KT-5823 (1 μM), 8Br-cGMP fails to evoke the marked reduction in [Ca 2+ ] i oscillations shown in ( A ). ( C ) Mean frequency values collected in the presence of 0.5 mM glucose (G), 8Br-cGMP plus 0.5 mM glucose (8Br-cGMP), KT-5823 (KT), and 8Br-cGMP plus KT-5823 (8Br-cGMP + KT). Results are representative of at least five cells in four different islets, expressed as mean ± SE.

    Journal: Environmental Health Perspectives

    Article Title: Low Doses of Bisphenol A and Diethylstilbestrol Impair Ca2+ Signals in Pancreatic ?-Cells through a Nonclassical Membrane Estrogen Receptor within Intact Islets of Langerhans

    doi: 10.1289/ehp.8002

    Figure Lengend Snippet: Effects of 8Br-cGMP on EDCs and E 2 via PKG. ( A ) Exposure to 10 μM 8Br-cGMP dramatically reduces the frequency of low-glucose–induced [Ca 2+ ] i oscillations. ( B ) After incubation with the specific PKG inhibitor KT-5823 (1 μM), 8Br-cGMP fails to evoke the marked reduction in [Ca 2+ ] i oscillations shown in ( A ). ( C ) Mean frequency values collected in the presence of 0.5 mM glucose (G), 8Br-cGMP plus 0.5 mM glucose (8Br-cGMP), KT-5823 (KT), and 8Br-cGMP plus KT-5823 (8Br-cGMP + KT). Results are representative of at least five cells in four different islets, expressed as mean ± SE.

    Article Snippet: Thus, KT-5823 does not alter [Ca2+ ]i oscillations but prevents the suppression of low-glucose–induced [Ca2+ ]i oscillations by BPA, indicating that BPA’s effect is exerted by a cGMP/PKG-mediated mechanism, as has been demonstrated for the natural hormone 17β-E2 ( ).

    Techniques: Incubation

    A–C . Conventional protein kinase C isoforms (PKCα,β,γ) effectively phosphorylate p75NTR and this phosphorylation is abolished by the kinase inhibitor K252a. A . Human p75NTR is phosphorylated in vitro by several kinases,

    Journal:

    Article Title: Fates of Neurotrophins after Retrograde Axonal Transport: Phosphorylation of p75NTR Is a Sorting Signal for Delayed Degradation

    doi: 10.1523/JNEUROSCI.2512-09.2009

    Figure Lengend Snippet: A–C . Conventional protein kinase C isoforms (PKCα,β,γ) effectively phosphorylate p75NTR and this phosphorylation is abolished by the kinase inhibitor K252a. A . Human p75NTR is phosphorylated in vitro by several kinases,

    Article Snippet: Therefore, we examined and compared the effects of intraocular injection of NGF alone (1 µg per eye), NGF with K252a (1 µat 250 µ), NGF with the conventional PKC inhibitor Gö6976 (1 µl at 250 µM), or K252a alone (1 µl at 250 µM), or Gö6976 alone (1 µl at 250 µM) on neuronal cell death in the ION using an established quantification protocol for pyknotic cells ( ; ; ; ; ).

    Techniques: In Vitro