anti kcnk3 task 1 antibody  (Alomone Labs)


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    Structured Review

    Alomone Labs anti kcnk3 task 1 antibody
    Anti Kcnk3 Task 1 Antibody, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    anti kcnk3 task 1 antibody - by Bioz Stars, 2022-08
    94/100 stars

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  • 94
    Alomone Labs rabbit anti kcnn2
    Effect of knockdown of <t>SK2</t> channels on aldosterone secretion in H295R cells (A) The mRNA expression of SK2 channels was reduced by 70% in H295R cells transduced with shRNA-SK2 lentiviruses. (B) knockdown of SK2 channels increased basal aldosterone secretion, and abrogated apamin-induced increase. *, P
    Rabbit Anti Kcnn2, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rabbit anti kcnn2/product/Alomone Labs
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    rabbit anti kcnn2 - by Bioz Stars, 2022-08
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    93
    Alomone Labs iberiotoxin
    For patch clamp experiments, undissociated CBs were used . The setup (A) and the image of the CB (B) are shown. Examples of K current records and mean I – V relationships in the DBA/2J (C) and the A/J mouse (D) are presented. Whole current traces were evoked by voltage steps from −80 to +60 mV with 10 mV increments for 100 ms. To construct a current–voltage curve, the mean current measured between 93 and 97 ms from the start of each voltage step. Mean currents were plotted versus the test pulse. Bars represent SEM. No statistical differences were seen between the strains. (E) The effect of <t>iberiotoxin</t> (a BK channel blocker; 200 nM) on outward current in GCs. The outward current was evoked by a test pulse from −80 to +20 mV for 100 ms. Iberiotoxin significantly and reversibly decreased outward current in GCs of the DBA/2J mice. However, K current in the A/J mice was not significantly affected by iberiotoxin. *, Significantly different from control and recovery. The amplitudes of K current (control and recovery) were significantly larger in GCs of the DBA/2J mice. Cont, control; Ibx, iberiotoxin; Rec, recovery.
    Iberiotoxin, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/iberiotoxin/product/Alomone Labs
    Average 93 stars, based on 1 article reviews
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    93
    Alomone Labs channel kir subunits kir2 1
    Inward rectifier K + current I K1 expression and function in transgenic atrial myocytes. Experimental animals involved wild‐type mice (WT) and transgenic mice (TG) expressing the repressor isoform of cyclic adenosine monophosphate response element modulator, CREM‐IbΔC‐X, and were distributed according to age in 6‐week‐old (WT 6w and TG 6w ) and 12‐week‐old (WT 12w and TG 12w ) groups. A , Representative traces of inward rectifiers K + channel (I K1 ) recorded in WT 12w and TG 12w myocytes before and after application of 10 μmol/L BaCl 2 using a ramp protocol (inset, scale bar 0.2 seconds). The traces show current densitiy (expressed in picoamperes/picofarads [pA/pF]) over time. ( Aa ). Current–voltage plots of I K1 averaged from traces of all cells. For WT 6w , n/N are 8/5, for TG 6w n/N are 10/6, for WT 12w n/N are 13/7, and for TG 12w n/N are 9/6 ( Ab ). B , Data show mean±SE of relative mRNA levels of atrial K + voltage‐gated channel subfamily J member 2 ( Kcnj2 ) and 4 ( Kcnj4 ) normalized to WT 6w vs Hprt1 (hypoxanthine phosphoribosyltransferase 1) as the housekeeping gene; N=8 per group. Y‐axes scale is Log2. Ca through c , Representative immunoblots ( Ca ) and quantification of K + inwardly rectifying channel subunits <t>Kir2.1</t> ( Cb ) and Kir2.3 ( Cc ) protein levels normalized to calsequestrin (CSQ). Data show mean±SE of 7 mice per group. Da through b , Representative action potentials recorded before (normal Tyrode [NT] as control) and after application of 10 μmol/L BaCl 2 in 1 atrial myocyte of each group ( Da ). Quantification of the percentages of action potential duration (APD) change in BaCl 2 vs NT at 50% (APD 50 ) and 90% (APD 90 ) repolarization. Data show mean±SE of n/N for WT 6w (7/5), TG 6w (5/5), WT 12w (11/6), and TG 12w (5/4) ( Db ). * P
    Channel Kir Subunits Kir2 1, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    Alomone Labs kir6 2
    Protein expression levels of Kir2.1 ( A ), Kir3.1 ( B ), and <t>Kir6.2</t> ( C ) channels in ipsilateral and contralateral hippocampi from both sham control and Aβ (1–42) -infused rat model. Upper panel: representative images of WB analysis on total protein extracts. β-actin was used as endogenous control for equal protein load. Lower panel: densitometric analysis of Kir2.1 ( A ), Kir3.1 ( B ), and Kir6.2 ( C ) protein levels. Data are expressed as fold change ratio on sham control and normalized to the β-actin protein levels. Bars represent the mean ± SEM obtained in 3 independent experiments, n = 7 for each group, * p
    Kir6 2, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    Effect of knockdown of SK2 channels on aldosterone secretion in H295R cells (A) The mRNA expression of SK2 channels was reduced by 70% in H295R cells transduced with shRNA-SK2 lentiviruses. (B) knockdown of SK2 channels increased basal aldosterone secretion, and abrogated apamin-induced increase. *, P

    Journal: Hypertension (Dallas, Tex. : 1979)

    Article Title: SK Channels Negatively Regulate Aldosterone Secretion in Human Adrenocortical Cells

    doi: 10.1161/HYPERTENSIONAHA.116.07094

    Figure Lengend Snippet: Effect of knockdown of SK2 channels on aldosterone secretion in H295R cells (A) The mRNA expression of SK2 channels was reduced by 70% in H295R cells transduced with shRNA-SK2 lentiviruses. (B) knockdown of SK2 channels increased basal aldosterone secretion, and abrogated apamin-induced increase. *, P

    Article Snippet: Slices were blocked (10% horse serum, 0.3% Triton X-100 in PBS, 2h, RT), and then incubated with primary antibody: rabbit anti-KCNN2 (1:200, Alomone Labs), 1% horse serum, 0.3% Triton X-100 in PBS, 1 day, 4°C.

    Techniques: Expressing, Transduction, shRNA

    H295R cells express functional SK channels (A-C) representative K + current traces in the absence and presence of SK channel activator 1-EBIO, or inhibitor apamin. (D) Apamin-sensitive SK channel currents obtained by subtracting C from B. (E) Current-voltage relationship ( I–V curves) generated from peak current density at each test voltage. Data are presented as means ± S.E.M. from 10 cells. (F) SK1, SK2 and SK3 channel mRNA expression were detected in H295R cells by RT-PCR. NTC: no template control.

    Journal: Hypertension (Dallas, Tex. : 1979)

    Article Title: SK Channels Negatively Regulate Aldosterone Secretion in Human Adrenocortical Cells

    doi: 10.1161/HYPERTENSIONAHA.116.07094

    Figure Lengend Snippet: H295R cells express functional SK channels (A-C) representative K + current traces in the absence and presence of SK channel activator 1-EBIO, or inhibitor apamin. (D) Apamin-sensitive SK channel currents obtained by subtracting C from B. (E) Current-voltage relationship ( I–V curves) generated from peak current density at each test voltage. Data are presented as means ± S.E.M. from 10 cells. (F) SK1, SK2 and SK3 channel mRNA expression were detected in H295R cells by RT-PCR. NTC: no template control.

    Article Snippet: Slices were blocked (10% horse serum, 0.3% Triton X-100 in PBS, 2h, RT), and then incubated with primary antibody: rabbit anti-KCNN2 (1:200, Alomone Labs), 1% horse serum, 0.3% Triton X-100 in PBS, 1 day, 4°C.

    Techniques: Functional Assay, Generated, Expressing, Reverse Transcription Polymerase Chain Reaction

    For patch clamp experiments, undissociated CBs were used . The setup (A) and the image of the CB (B) are shown. Examples of K current records and mean I – V relationships in the DBA/2J (C) and the A/J mouse (D) are presented. Whole current traces were evoked by voltage steps from −80 to +60 mV with 10 mV increments for 100 ms. To construct a current–voltage curve, the mean current measured between 93 and 97 ms from the start of each voltage step. Mean currents were plotted versus the test pulse. Bars represent SEM. No statistical differences were seen between the strains. (E) The effect of iberiotoxin (a BK channel blocker; 200 nM) on outward current in GCs. The outward current was evoked by a test pulse from −80 to +20 mV for 100 ms. Iberiotoxin significantly and reversibly decreased outward current in GCs of the DBA/2J mice. However, K current in the A/J mice was not significantly affected by iberiotoxin. *, Significantly different from control and recovery. The amplitudes of K current (control and recovery) were significantly larger in GCs of the DBA/2J mice. Cont, control; Ibx, iberiotoxin; Rec, recovery.

    Journal: Frontiers in Cellular Neuroscience

    Article Title: Differential Expression of Large-Conductance Ca2+-Activated K Channels in the Carotid Body between DBA/2J and A/J Strains of Mice

    doi: 10.3389/fncel.2011.00019

    Figure Lengend Snippet: For patch clamp experiments, undissociated CBs were used . The setup (A) and the image of the CB (B) are shown. Examples of K current records and mean I – V relationships in the DBA/2J (C) and the A/J mouse (D) are presented. Whole current traces were evoked by voltage steps from −80 to +60 mV with 10 mV increments for 100 ms. To construct a current–voltage curve, the mean current measured between 93 and 97 ms from the start of each voltage step. Mean currents were plotted versus the test pulse. Bars represent SEM. No statistical differences were seen between the strains. (E) The effect of iberiotoxin (a BK channel blocker; 200 nM) on outward current in GCs. The outward current was evoked by a test pulse from −80 to +20 mV for 100 ms. Iberiotoxin significantly and reversibly decreased outward current in GCs of the DBA/2J mice. However, K current in the A/J mice was not significantly affected by iberiotoxin. *, Significantly different from control and recovery. The amplitudes of K current (control and recovery) were significantly larger in GCs of the DBA/2J mice. Cont, control; Ibx, iberiotoxin; Rec, recovery.

    Article Snippet: To examine the BK channel component, a single step protocol was applied with or without iberiotoxin (200 nM; Alomone Laboratories), a selective blocker for BK channels.

    Techniques: Patch Clamp, Construct, Mouse Assay

    Inward rectifier K + current I K1 expression and function in transgenic atrial myocytes. Experimental animals involved wild‐type mice (WT) and transgenic mice (TG) expressing the repressor isoform of cyclic adenosine monophosphate response element modulator, CREM‐IbΔC‐X, and were distributed according to age in 6‐week‐old (WT 6w and TG 6w ) and 12‐week‐old (WT 12w and TG 12w ) groups. A , Representative traces of inward rectifiers K + channel (I K1 ) recorded in WT 12w and TG 12w myocytes before and after application of 10 μmol/L BaCl 2 using a ramp protocol (inset, scale bar 0.2 seconds). The traces show current densitiy (expressed in picoamperes/picofarads [pA/pF]) over time. ( Aa ). Current–voltage plots of I K1 averaged from traces of all cells. For WT 6w , n/N are 8/5, for TG 6w n/N are 10/6, for WT 12w n/N are 13/7, and for TG 12w n/N are 9/6 ( Ab ). B , Data show mean±SE of relative mRNA levels of atrial K + voltage‐gated channel subfamily J member 2 ( Kcnj2 ) and 4 ( Kcnj4 ) normalized to WT 6w vs Hprt1 (hypoxanthine phosphoribosyltransferase 1) as the housekeeping gene; N=8 per group. Y‐axes scale is Log2. Ca through c , Representative immunoblots ( Ca ) and quantification of K + inwardly rectifying channel subunits Kir2.1 ( Cb ) and Kir2.3 ( Cc ) protein levels normalized to calsequestrin (CSQ). Data show mean±SE of 7 mice per group. Da through b , Representative action potentials recorded before (normal Tyrode [NT] as control) and after application of 10 μmol/L BaCl 2 in 1 atrial myocyte of each group ( Da ). Quantification of the percentages of action potential duration (APD) change in BaCl 2 vs NT at 50% (APD 50 ) and 90% (APD 90 ) repolarization. Data show mean±SE of n/N for WT 6w (7/5), TG 6w (5/5), WT 12w (11/6), and TG 12w (5/4) ( Db ). * P

    Journal: Journal of the American Heart Association: Cardiovascular and Cerebrovascular Disease

    Article Title: Inward Rectifier K+ Currents Contribute to the Proarrhythmic Electrical Phenotype of Atria Overexpressing Cyclic Adenosine Monophosphate Response Element Modulator Isoform CREM‐IbΔC‐X

    doi: 10.1161/JAHA.119.016144

    Figure Lengend Snippet: Inward rectifier K + current I K1 expression and function in transgenic atrial myocytes. Experimental animals involved wild‐type mice (WT) and transgenic mice (TG) expressing the repressor isoform of cyclic adenosine monophosphate response element modulator, CREM‐IbΔC‐X, and were distributed according to age in 6‐week‐old (WT 6w and TG 6w ) and 12‐week‐old (WT 12w and TG 12w ) groups. A , Representative traces of inward rectifiers K + channel (I K1 ) recorded in WT 12w and TG 12w myocytes before and after application of 10 μmol/L BaCl 2 using a ramp protocol (inset, scale bar 0.2 seconds). The traces show current densitiy (expressed in picoamperes/picofarads [pA/pF]) over time. ( Aa ). Current–voltage plots of I K1 averaged from traces of all cells. For WT 6w , n/N are 8/5, for TG 6w n/N are 10/6, for WT 12w n/N are 13/7, and for TG 12w n/N are 9/6 ( Ab ). B , Data show mean±SE of relative mRNA levels of atrial K + voltage‐gated channel subfamily J member 2 ( Kcnj2 ) and 4 ( Kcnj4 ) normalized to WT 6w vs Hprt1 (hypoxanthine phosphoribosyltransferase 1) as the housekeeping gene; N=8 per group. Y‐axes scale is Log2. Ca through c , Representative immunoblots ( Ca ) and quantification of K + inwardly rectifying channel subunits Kir2.1 ( Cb ) and Kir2.3 ( Cc ) protein levels normalized to calsequestrin (CSQ). Data show mean±SE of 7 mice per group. Da through b , Representative action potentials recorded before (normal Tyrode [NT] as control) and after application of 10 μmol/L BaCl 2 in 1 atrial myocyte of each group ( Da ). Quantification of the percentages of action potential duration (APD) change in BaCl 2 vs NT at 50% (APD 50 ) and 90% (APD 90 ) repolarization. Data show mean±SE of n/N for WT 6w (7/5), TG 6w (5/5), WT 12w (11/6), and TG 12w (5/4) ( Db ). * P

    Article Snippet: The following rabbit polyclonal primary antibodies were used against K + voltage‐gated channels (Kv) subunits Kv4.2 (1:200, APC 023, Alomone Labs, Jerusalem, Israel), Kv4.3 (1:200, APC 017, Alomone Labs), K + channel interacting protein 2 (KChIP2) (1:200, sc‐25685, Santa Cruz Biotechnology Inc, Santa Cruz, CA), and K + inwardly rectifying channel (Kir) subunits Kir2.1 (1:200, APC 159, Alomone Labs), Kir2.3 (1:1000, APC 032, Alomone Labs), Kir3.1 (1:200, APC 005, Alomone Labs), Kir3.4 (1:200, APC 027, Alomone Labs), and as loading control calsequestrin (1:2500, PA1‐913, Thermo Fisher Scientific).

    Techniques: Expressing, Transgenic Assay, Mouse Assay, Western Blot

    Protein expression levels of Kir2.1 ( A ), Kir3.1 ( B ), and Kir6.2 ( C ) channels in ipsilateral and contralateral hippocampi from both sham control and Aβ (1–42) -infused rat model. Upper panel: representative images of WB analysis on total protein extracts. β-actin was used as endogenous control for equal protein load. Lower panel: densitometric analysis of Kir2.1 ( A ), Kir3.1 ( B ), and Kir6.2 ( C ) protein levels. Data are expressed as fold change ratio on sham control and normalized to the β-actin protein levels. Bars represent the mean ± SEM obtained in 3 independent experiments, n = 7 for each group, * p

    Journal: Biomedicines

    Article Title: Unraveling the Role of Inwardly Rectifying Potassium Channels in the Hippocampus of an Aβ(1–42)-Infused Rat Model of Alzheimer’s Disease

    doi: 10.3390/biomedicines8030058

    Figure Lengend Snippet: Protein expression levels of Kir2.1 ( A ), Kir3.1 ( B ), and Kir6.2 ( C ) channels in ipsilateral and contralateral hippocampi from both sham control and Aβ (1–42) -infused rat model. Upper panel: representative images of WB analysis on total protein extracts. β-actin was used as endogenous control for equal protein load. Lower panel: densitometric analysis of Kir2.1 ( A ), Kir3.1 ( B ), and Kir6.2 ( C ) protein levels. Data are expressed as fold change ratio on sham control and normalized to the β-actin protein levels. Bars represent the mean ± SEM obtained in 3 independent experiments, n = 7 for each group, * p

    Article Snippet: Then, the membranes were blocked with 5% milk solution prepared in TBST (Tris-buffered saline, 0.1% Tween 20) buffer and incubated with one of the following primary antibodies against Kir2.1 (rabbit polyclonal, 1:200, Abcam, Cambridge, UK [ab65796]), Kir3.1 (mouse monoclonal, 1:200, Alomone Labs, Jerusalem, Israel [APC-005]), Kir6.2 (rabbit polyclonal, 1:200, Alomone Labs, Jerusalem, Israel [APC-020]), or β-actin (mouse monoclonal, 1:5000, Thermo Fisher Scientific, Waltham, MA, USA [AC-15]).

    Techniques: Expressing, Western Blot

    Relative mRNA levels of ( A ) Kcnj2 (Kir2.1), ( B ) Kcnj3 (Kir3.1), and ( C ) Kcnj11 (Kir6.2) in ipsilateral and contralateral hippocampi from both sham control and Aβ (1–42) -infused rat model. Data are expressed as fold change of mRNA levels normalized to the housekeeping control gene ( Gapdh ) and represent the mean ± SEM obtained in 3 independent experiments, n = 7 for each group, * p

    Journal: Biomedicines

    Article Title: Unraveling the Role of Inwardly Rectifying Potassium Channels in the Hippocampus of an Aβ(1–42)-Infused Rat Model of Alzheimer’s Disease

    doi: 10.3390/biomedicines8030058

    Figure Lengend Snippet: Relative mRNA levels of ( A ) Kcnj2 (Kir2.1), ( B ) Kcnj3 (Kir3.1), and ( C ) Kcnj11 (Kir6.2) in ipsilateral and contralateral hippocampi from both sham control and Aβ (1–42) -infused rat model. Data are expressed as fold change of mRNA levels normalized to the housekeeping control gene ( Gapdh ) and represent the mean ± SEM obtained in 3 independent experiments, n = 7 for each group, * p

    Article Snippet: Then, the membranes were blocked with 5% milk solution prepared in TBST (Tris-buffered saline, 0.1% Tween 20) buffer and incubated with one of the following primary antibodies against Kir2.1 (rabbit polyclonal, 1:200, Abcam, Cambridge, UK [ab65796]), Kir3.1 (mouse monoclonal, 1:200, Alomone Labs, Jerusalem, Israel [APC-005]), Kir6.2 (rabbit polyclonal, 1:200, Alomone Labs, Jerusalem, Israel [APC-020]), or β-actin (mouse monoclonal, 1:5000, Thermo Fisher Scientific, Waltham, MA, USA [AC-15]).

    Techniques: