k252a  (Alomone Labs)


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    Alomone Labs k252a
    ( A ) Validation of the cdk1 sandwich ELISA assay performed with 10 fg of either GST (GST) or a chimeric protein containing the sequence of human cdk1 bound to GST (Cdk1-GST), and revealed with the anti-cdk1 PSTAIRE antibody. Normalized optical density (O.D.) at 405 nm is shown. ( B ) Validation of the phospho-cdk1 sandwich ELISA assay performed with cell lysates obtained from DCRNs cultured in the presence of 100 ng/ml NGF and 2 ng/ml BDNF, incubated either in the absence (- CIP) or presence (+ CIP) of CIP, and revealed with the anti-cdk1 (pTyr15) antibody. Normalized optical density (O.D.) at 405 nm is shown. ( C ) Cell lysates from DCRNs cultured in the presence (+) or absence (-) of the referred factors were subjected to sandwich ELISA analyses using antibodies specific for either cdk1 phosphorylated at Tyr15 or total cdk1 protein. Colorimetric values for cdk1 phosphorylated at Tyr15 were normalized to the levels of cdk1 protein (Cdk1). ( D ) Cell lysates from DCRNs cultured in the presence of the referred factors and/or <t>K252a</t> were subjected to sandwich ELISA analyses as described above. Colorimetric values for cdk1 phosphorylated at Tyr15 were normalized to the levels of cdk1 protein (Cdk1). *p<0.05; ***p<0.005 (Student’s t test; n = 3).
    K252a, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/k252a/product/Alomone Labs
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    k252a - by Bioz Stars, 2023-01
    94/100 stars

    Images

    1) Product Images from "Brain-Derived Neurotrophic Factor-Dependent cdk1 Inhibition Prevents G2/M Progression in Differentiating Tetraploid Neurons"

    Article Title: Brain-Derived Neurotrophic Factor-Dependent cdk1 Inhibition Prevents G2/M Progression in Differentiating Tetraploid Neurons

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0064890

    ( A ) Validation of the cdk1 sandwich ELISA assay performed with 10 fg of either GST (GST) or a chimeric protein containing the sequence of human cdk1 bound to GST (Cdk1-GST), and revealed with the anti-cdk1 PSTAIRE antibody. Normalized optical density (O.D.) at 405 nm is shown. ( B ) Validation of the phospho-cdk1 sandwich ELISA assay performed with cell lysates obtained from DCRNs cultured in the presence of 100 ng/ml NGF and 2 ng/ml BDNF, incubated either in the absence (- CIP) or presence (+ CIP) of CIP, and revealed with the anti-cdk1 (pTyr15) antibody. Normalized optical density (O.D.) at 405 nm is shown. ( C ) Cell lysates from DCRNs cultured in the presence (+) or absence (-) of the referred factors were subjected to sandwich ELISA analyses using antibodies specific for either cdk1 phosphorylated at Tyr15 or total cdk1 protein. Colorimetric values for cdk1 phosphorylated at Tyr15 were normalized to the levels of cdk1 protein (Cdk1). ( D ) Cell lysates from DCRNs cultured in the presence of the referred factors and/or K252a were subjected to sandwich ELISA analyses as described above. Colorimetric values for cdk1 phosphorylated at Tyr15 were normalized to the levels of cdk1 protein (Cdk1). *p<0.05; ***p<0.005 (Student’s t test; n = 3).
    Figure Legend Snippet: ( A ) Validation of the cdk1 sandwich ELISA assay performed with 10 fg of either GST (GST) or a chimeric protein containing the sequence of human cdk1 bound to GST (Cdk1-GST), and revealed with the anti-cdk1 PSTAIRE antibody. Normalized optical density (O.D.) at 405 nm is shown. ( B ) Validation of the phospho-cdk1 sandwich ELISA assay performed with cell lysates obtained from DCRNs cultured in the presence of 100 ng/ml NGF and 2 ng/ml BDNF, incubated either in the absence (- CIP) or presence (+ CIP) of CIP, and revealed with the anti-cdk1 (pTyr15) antibody. Normalized optical density (O.D.) at 405 nm is shown. ( C ) Cell lysates from DCRNs cultured in the presence (+) or absence (-) of the referred factors were subjected to sandwich ELISA analyses using antibodies specific for either cdk1 phosphorylated at Tyr15 or total cdk1 protein. Colorimetric values for cdk1 phosphorylated at Tyr15 were normalized to the levels of cdk1 protein (Cdk1). ( D ) Cell lysates from DCRNs cultured in the presence of the referred factors and/or K252a were subjected to sandwich ELISA analyses as described above. Colorimetric values for cdk1 phosphorylated at Tyr15 were normalized to the levels of cdk1 protein (Cdk1). *p<0.05; ***p<0.005 (Student’s t test; n = 3).

    Techniques Used: Sandwich ELISA, Sequencing, Cell Culture, Incubation

    k252a  (Alomone Labs)


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    Alomone Labs k252a
    ( A ) Validation of the cdk1 sandwich ELISA assay performed with 10 fg of either GST (GST) or a chimeric protein containing the sequence of human cdk1 bound to GST (Cdk1-GST), and revealed with the anti-cdk1 PSTAIRE antibody. Normalized optical density (O.D.) at 405 nm is shown. ( B ) Validation of the phospho-cdk1 sandwich ELISA assay performed with cell lysates obtained from DCRNs cultured in the presence of 100 ng/ml NGF and 2 ng/ml BDNF, incubated either in the absence (- CIP) or presence (+ CIP) of CIP, and revealed with the anti-cdk1 (pTyr15) antibody. Normalized optical density (O.D.) at 405 nm is shown. ( C ) Cell lysates from DCRNs cultured in the presence (+) or absence (-) of the referred factors were subjected to sandwich ELISA analyses using antibodies specific for either cdk1 phosphorylated at Tyr15 or total cdk1 protein. Colorimetric values for cdk1 phosphorylated at Tyr15 were normalized to the levels of cdk1 protein (Cdk1). ( D ) Cell lysates from DCRNs cultured in the presence of the referred factors and/or <t>K252a</t> were subjected to sandwich ELISA analyses as described above. Colorimetric values for cdk1 phosphorylated at Tyr15 were normalized to the levels of cdk1 protein (Cdk1). *p<0.05; ***p<0.005 (Student’s t test; n = 3).
    K252a, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/k252a/product/Alomone Labs
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    k252a - by Bioz Stars, 2023-01
    94/100 stars

    Images

    1) Product Images from "Brain-Derived Neurotrophic Factor-Dependent cdk1 Inhibition Prevents G2/M Progression in Differentiating Tetraploid Neurons"

    Article Title: Brain-Derived Neurotrophic Factor-Dependent cdk1 Inhibition Prevents G2/M Progression in Differentiating Tetraploid Neurons

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0064890

    ( A ) Validation of the cdk1 sandwich ELISA assay performed with 10 fg of either GST (GST) or a chimeric protein containing the sequence of human cdk1 bound to GST (Cdk1-GST), and revealed with the anti-cdk1 PSTAIRE antibody. Normalized optical density (O.D.) at 405 nm is shown. ( B ) Validation of the phospho-cdk1 sandwich ELISA assay performed with cell lysates obtained from DCRNs cultured in the presence of 100 ng/ml NGF and 2 ng/ml BDNF, incubated either in the absence (- CIP) or presence (+ CIP) of CIP, and revealed with the anti-cdk1 (pTyr15) antibody. Normalized optical density (O.D.) at 405 nm is shown. ( C ) Cell lysates from DCRNs cultured in the presence (+) or absence (-) of the referred factors were subjected to sandwich ELISA analyses using antibodies specific for either cdk1 phosphorylated at Tyr15 or total cdk1 protein. Colorimetric values for cdk1 phosphorylated at Tyr15 were normalized to the levels of cdk1 protein (Cdk1). ( D ) Cell lysates from DCRNs cultured in the presence of the referred factors and/or K252a were subjected to sandwich ELISA analyses as described above. Colorimetric values for cdk1 phosphorylated at Tyr15 were normalized to the levels of cdk1 protein (Cdk1). *p<0.05; ***p<0.005 (Student’s t test; n = 3).
    Figure Legend Snippet: ( A ) Validation of the cdk1 sandwich ELISA assay performed with 10 fg of either GST (GST) or a chimeric protein containing the sequence of human cdk1 bound to GST (Cdk1-GST), and revealed with the anti-cdk1 PSTAIRE antibody. Normalized optical density (O.D.) at 405 nm is shown. ( B ) Validation of the phospho-cdk1 sandwich ELISA assay performed with cell lysates obtained from DCRNs cultured in the presence of 100 ng/ml NGF and 2 ng/ml BDNF, incubated either in the absence (- CIP) or presence (+ CIP) of CIP, and revealed with the anti-cdk1 (pTyr15) antibody. Normalized optical density (O.D.) at 405 nm is shown. ( C ) Cell lysates from DCRNs cultured in the presence (+) or absence (-) of the referred factors were subjected to sandwich ELISA analyses using antibodies specific for either cdk1 phosphorylated at Tyr15 or total cdk1 protein. Colorimetric values for cdk1 phosphorylated at Tyr15 were normalized to the levels of cdk1 protein (Cdk1). ( D ) Cell lysates from DCRNs cultured in the presence of the referred factors and/or K252a were subjected to sandwich ELISA analyses as described above. Colorimetric values for cdk1 phosphorylated at Tyr15 were normalized to the levels of cdk1 protein (Cdk1). *p<0.05; ***p<0.005 (Student’s t test; n = 3).

    Techniques Used: Sandwich ELISA, Sequencing, Cell Culture, Incubation

    k252a  (Alomone Labs)


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    Alomone Labs k252a
    BDNF release and effect of exogenous BDNF, pro-BDNF, TrkB inhibitor <t> (K252a) </t> and neutralizing anti-BDNF on apoptosis and proliferation in primary CRC cell lines.
    K252a, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/k252a/product/Alomone Labs
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    k252a - by Bioz Stars, 2023-01
    94/100 stars

    Images

    1) Product Images from "Fine-Tuning Roles of Endogenous Brain-Derived Neurotrophic Factor, TrkB and Sortilin in Colorectal Cancer Cell Survival"

    Article Title: Fine-Tuning Roles of Endogenous Brain-Derived Neurotrophic Factor, TrkB and Sortilin in Colorectal Cancer Cell Survival

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0025097

    BDNF release and effect of exogenous BDNF, pro-BDNF, TrkB inhibitor  (K252a)  and neutralizing anti-BDNF on apoptosis and proliferation in primary CRC cell lines.
    Figure Legend Snippet: BDNF release and effect of exogenous BDNF, pro-BDNF, TrkB inhibitor (K252a) and neutralizing anti-BDNF on apoptosis and proliferation in primary CRC cell lines.

    Techniques Used:

    BDNF release and effect of exogenous BDNF, pro-BDNF, TrkB inhibitor  (K252a)  and neutralizing anti-BDNF on apoptosis and proliferation in metastatic CRC cell lines.
    Figure Legend Snippet: BDNF release and effect of exogenous BDNF, pro-BDNF, TrkB inhibitor (K252a) and neutralizing anti-BDNF on apoptosis and proliferation in metastatic CRC cell lines.

    Techniques Used:

    (A) Role of endogenous BDNF and its receptor TrkB on CRC cell proliferation: effects of exogenous BDNF and suppressing endogenous TrkB receptor on cell proliferation. The four cell lines were cultured for 24 h in FCS-free medium (FCS 10%, −) in the presence of exogenous BDNF (+), K252a (+) alone or in combination. Cell proliferation was determined by flow cytometry analysis using EdU Alexa Fluor 488. The data are presented as histograms of proliferating cells in relative units ± SEM of five independent experiments. *, p <0.05; **, p <0.01; ***, p <0.001, when compared with serum-free medium. (B, C) Effects of exogenous BDNF and suppressing endogenous TrkB receptor on cell survival. Apoptotic ratios of soluble nucleosomes were detected by ELISA Cell for WiDr, SW480, SW620, and COLO 205 induced by serum deprivation alone (FCS 10%, −) or in association either with exogenous BDNF (+), or with K252a (+), during 24–72 h of serum deprivation. Histograms, mean ratio of apoptotic cells ± SEM of at least three independent experiments. *, p <0.05; **, p <0.01; ***, p <0.001, when compared with serum-free condition alone. (D, E) apoptotic ratio after 24 h serum deprivation alone (0% FCS) or with combination with a neutralizing anti-BDNF mAb (0% anti-BDNF). Histograms, mean ratio of apoptotic cells ± SEM of three independent experiments. *, p <0.05; **, p <0.01; ***, p <0.001, when compared with serum-free condition alone.
    Figure Legend Snippet: (A) Role of endogenous BDNF and its receptor TrkB on CRC cell proliferation: effects of exogenous BDNF and suppressing endogenous TrkB receptor on cell proliferation. The four cell lines were cultured for 24 h in FCS-free medium (FCS 10%, −) in the presence of exogenous BDNF (+), K252a (+) alone or in combination. Cell proliferation was determined by flow cytometry analysis using EdU Alexa Fluor 488. The data are presented as histograms of proliferating cells in relative units ± SEM of five independent experiments. *, p <0.05; **, p <0.01; ***, p <0.001, when compared with serum-free medium. (B, C) Effects of exogenous BDNF and suppressing endogenous TrkB receptor on cell survival. Apoptotic ratios of soluble nucleosomes were detected by ELISA Cell for WiDr, SW480, SW620, and COLO 205 induced by serum deprivation alone (FCS 10%, −) or in association either with exogenous BDNF (+), or with K252a (+), during 24–72 h of serum deprivation. Histograms, mean ratio of apoptotic cells ± SEM of at least three independent experiments. *, p <0.05; **, p <0.01; ***, p <0.001, when compared with serum-free condition alone. (D, E) apoptotic ratio after 24 h serum deprivation alone (0% FCS) or with combination with a neutralizing anti-BDNF mAb (0% anti-BDNF). Histograms, mean ratio of apoptotic cells ± SEM of three independent experiments. *, p <0.05; **, p <0.01; ***, p <0.001, when compared with serum-free condition alone.

    Techniques Used: Cell Culture, Flow Cytometry, Enzyme-linked Immunosorbent Assay

    k 252a  (Alomone Labs)


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    Alomone Labs k 252a
    Role of Trk and p75 NTR in BDNF's effects on dendritic spine density and morphology. (a) Representative examples of dendritic segments of CA1 pyramidal neurons maintained in serum-containing media (SM) and treated with the function-blocking antibody of p75 NTR , REX (50 μ g/mL), and BDNF (250 ng/mL) for 48 hrs (scale bar represents 2 μ m). (b) Dendritic spine density expressed per 10 μ m of apical dendrite. (c) Proportion of each morphological type of dendritic spine, expressed as a fraction of the total spine population. (d) Representative examples of dendritic segments of CA1 pyramidal neurons maintained in SM and treated with <t>k-252a</t> (200 nM) and BDNF (250 ng/mL) for 48 hrs. (e) Dendritic spine density expressed per 10 μ m of apical dendrite. (f) Proportion of each morphological type of dendritic spines, expressed as a fraction of the total spine population. * P < 0.05, ** P < 0.01, and *** P < 0.001, after a one-way ANOVA.
    K 252a, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/k 252a/product/Alomone Labs
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    k 252a - by Bioz Stars, 2023-01
    94/100 stars

    Images

    1) Product Images from "Divergent Roles of p75 NTR and Trk Receptors in BDNF's Effects on Dendritic Spine Density and Morphology"

    Article Title: Divergent Roles of p75 NTR and Trk Receptors in BDNF's Effects on Dendritic Spine Density and Morphology

    Journal: Neural Plasticity

    doi: 10.1155/2012/578057

    Role of Trk and p75 NTR in BDNF's effects on dendritic spine density and morphology. (a) Representative examples of dendritic segments of CA1 pyramidal neurons maintained in serum-containing media (SM) and treated with the function-blocking antibody of p75 NTR , REX (50 μ g/mL), and BDNF (250 ng/mL) for 48 hrs (scale bar represents 2 μ m). (b) Dendritic spine density expressed per 10 μ m of apical dendrite. (c) Proportion of each morphological type of dendritic spine, expressed as a fraction of the total spine population. (d) Representative examples of dendritic segments of CA1 pyramidal neurons maintained in SM and treated with k-252a (200 nM) and BDNF (250 ng/mL) for 48 hrs. (e) Dendritic spine density expressed per 10 μ m of apical dendrite. (f) Proportion of each morphological type of dendritic spines, expressed as a fraction of the total spine population. * P < 0.05, ** P < 0.01, and *** P < 0.001, after a one-way ANOVA.
    Figure Legend Snippet: Role of Trk and p75 NTR in BDNF's effects on dendritic spine density and morphology. (a) Representative examples of dendritic segments of CA1 pyramidal neurons maintained in serum-containing media (SM) and treated with the function-blocking antibody of p75 NTR , REX (50 μ g/mL), and BDNF (250 ng/mL) for 48 hrs (scale bar represents 2 μ m). (b) Dendritic spine density expressed per 10 μ m of apical dendrite. (c) Proportion of each morphological type of dendritic spine, expressed as a fraction of the total spine population. (d) Representative examples of dendritic segments of CA1 pyramidal neurons maintained in SM and treated with k-252a (200 nM) and BDNF (250 ng/mL) for 48 hrs. (e) Dendritic spine density expressed per 10 μ m of apical dendrite. (f) Proportion of each morphological type of dendritic spines, expressed as a fraction of the total spine population. * P < 0.05, ** P < 0.01, and *** P < 0.001, after a one-way ANOVA.

    Techniques Used: Blocking Assay

    Role of neuronal activity in k-252a's effects on dendritic spine density and morphology. (a) Representative examples of dendritic segments of CA1 pyramidal neurons maintained in serum-containing media (SM) and treated with k-252a (200 nM), TTX (1 μ M), or both k-252a and TTX for 48 hrs (scale bar represents 2 μ m). (b) Dendritic spine density expressed per 10 μ m of apical dendrite. (c) Proportion of each morphological type of dendritic spines, expressed as a fraction of the total spine population. * P < 0.05, ** P < 0.01, and *** P < 0.001, after a one-way ANOVA.
    Figure Legend Snippet: Role of neuronal activity in k-252a's effects on dendritic spine density and morphology. (a) Representative examples of dendritic segments of CA1 pyramidal neurons maintained in serum-containing media (SM) and treated with k-252a (200 nM), TTX (1 μ M), or both k-252a and TTX for 48 hrs (scale bar represents 2 μ m). (b) Dendritic spine density expressed per 10 μ m of apical dendrite. (c) Proportion of each morphological type of dendritic spines, expressed as a fraction of the total spine population. * P < 0.05, ** P < 0.01, and *** P < 0.001, after a one-way ANOVA.

    Techniques Used: Activity Assay

    Quantitative results of dendritic spine analyses .
    Figure Legend Snippet: Quantitative results of dendritic spine analyses .

    Techniques Used:

    Summary of total dendritic length, cells, slices, and individual spines sampled for the quantitative dendritic spine analyses.
    Figure Legend Snippet: Summary of total dendritic length, cells, slices, and individual spines sampled for the quantitative dendritic spine analyses.

    Techniques Used:

    k 252a  (Alomone Labs)


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    Alomone Labs k 252a
    Role of Trk and p75 NTR in BDNF's effects on dendritic spine density and morphology. (a) Representative examples of dendritic segments of CA1 pyramidal neurons maintained in serum-containing media (SM) and treated with the function-blocking antibody of p75 NTR , REX (50 μ g/mL), and BDNF (250 ng/mL) for 48 hrs (scale bar represents 2 μ m). (b) Dendritic spine density expressed per 10 μ m of apical dendrite. (c) Proportion of each morphological type of dendritic spine, expressed as a fraction of the total spine population. (d) Representative examples of dendritic segments of CA1 pyramidal neurons maintained in SM and treated with <t>k-252a</t> (200 nM) and BDNF (250 ng/mL) for 48 hrs. (e) Dendritic spine density expressed per 10 μ m of apical dendrite. (f) Proportion of each morphological type of dendritic spines, expressed as a fraction of the total spine population. * P < 0.05, ** P < 0.01, and *** P < 0.001, after a one-way ANOVA.
    K 252a, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/k 252a/product/Alomone Labs
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    k 252a - by Bioz Stars, 2023-01
    94/100 stars

    Images

    1) Product Images from "Divergent Roles of p75 NTR and Trk Receptors in BDNF's Effects on Dendritic Spine Density and Morphology"

    Article Title: Divergent Roles of p75 NTR and Trk Receptors in BDNF's Effects on Dendritic Spine Density and Morphology

    Journal: Neural Plasticity

    doi: 10.1155/2012/578057

    Role of Trk and p75 NTR in BDNF's effects on dendritic spine density and morphology. (a) Representative examples of dendritic segments of CA1 pyramidal neurons maintained in serum-containing media (SM) and treated with the function-blocking antibody of p75 NTR , REX (50 μ g/mL), and BDNF (250 ng/mL) for 48 hrs (scale bar represents 2 μ m). (b) Dendritic spine density expressed per 10 μ m of apical dendrite. (c) Proportion of each morphological type of dendritic spine, expressed as a fraction of the total spine population. (d) Representative examples of dendritic segments of CA1 pyramidal neurons maintained in SM and treated with k-252a (200 nM) and BDNF (250 ng/mL) for 48 hrs. (e) Dendritic spine density expressed per 10 μ m of apical dendrite. (f) Proportion of each morphological type of dendritic spines, expressed as a fraction of the total spine population. * P < 0.05, ** P < 0.01, and *** P < 0.001, after a one-way ANOVA.
    Figure Legend Snippet: Role of Trk and p75 NTR in BDNF's effects on dendritic spine density and morphology. (a) Representative examples of dendritic segments of CA1 pyramidal neurons maintained in serum-containing media (SM) and treated with the function-blocking antibody of p75 NTR , REX (50 μ g/mL), and BDNF (250 ng/mL) for 48 hrs (scale bar represents 2 μ m). (b) Dendritic spine density expressed per 10 μ m of apical dendrite. (c) Proportion of each morphological type of dendritic spine, expressed as a fraction of the total spine population. (d) Representative examples of dendritic segments of CA1 pyramidal neurons maintained in SM and treated with k-252a (200 nM) and BDNF (250 ng/mL) for 48 hrs. (e) Dendritic spine density expressed per 10 μ m of apical dendrite. (f) Proportion of each morphological type of dendritic spines, expressed as a fraction of the total spine population. * P < 0.05, ** P < 0.01, and *** P < 0.001, after a one-way ANOVA.

    Techniques Used: Blocking Assay

    Role of neuronal activity in k-252a's effects on dendritic spine density and morphology. (a) Representative examples of dendritic segments of CA1 pyramidal neurons maintained in serum-containing media (SM) and treated with k-252a (200 nM), TTX (1 μ M), or both k-252a and TTX for 48 hrs (scale bar represents 2 μ m). (b) Dendritic spine density expressed per 10 μ m of apical dendrite. (c) Proportion of each morphological type of dendritic spines, expressed as a fraction of the total spine population. * P < 0.05, ** P < 0.01, and *** P < 0.001, after a one-way ANOVA.
    Figure Legend Snippet: Role of neuronal activity in k-252a's effects on dendritic spine density and morphology. (a) Representative examples of dendritic segments of CA1 pyramidal neurons maintained in serum-containing media (SM) and treated with k-252a (200 nM), TTX (1 μ M), or both k-252a and TTX for 48 hrs (scale bar represents 2 μ m). (b) Dendritic spine density expressed per 10 μ m of apical dendrite. (c) Proportion of each morphological type of dendritic spines, expressed as a fraction of the total spine population. * P < 0.05, ** P < 0.01, and *** P < 0.001, after a one-way ANOVA.

    Techniques Used: Activity Assay

    Quantitative results of dendritic spine analyses .
    Figure Legend Snippet: Quantitative results of dendritic spine analyses .

    Techniques Used:

    Summary of total dendritic length, cells, slices, and individual spines sampled for the quantitative dendritic spine analyses.
    Figure Legend Snippet: Summary of total dendritic length, cells, slices, and individual spines sampled for the quantitative dendritic spine analyses.

    Techniques Used:

    k 252a  (Alomone Labs)


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    Alomone Labs k 252a
    Role of Trk and p75 NTR in BDNF's effects on dendritic spine density and morphology. (a) Representative examples of dendritic segments of CA1 pyramidal neurons maintained in serum-containing media (SM) and treated with the function-blocking antibody of p75 NTR , REX (50 μ g/mL), and BDNF (250 ng/mL) for 48 hrs (scale bar represents 2 μ m). (b) Dendritic spine density expressed per 10 μ m of apical dendrite. (c) Proportion of each morphological type of dendritic spine, expressed as a fraction of the total spine population. (d) Representative examples of dendritic segments of CA1 pyramidal neurons maintained in SM and treated with <t>k-252a</t> (200 nM) and BDNF (250 ng/mL) for 48 hrs. (e) Dendritic spine density expressed per 10 μ m of apical dendrite. (f) Proportion of each morphological type of dendritic spines, expressed as a fraction of the total spine population. * P < 0.05, ** P < 0.01, and *** P < 0.001, after a one-way ANOVA.
    K 252a, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/k 252a/product/Alomone Labs
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    k 252a - by Bioz Stars, 2023-01
    94/100 stars

    Images

    1) Product Images from "Divergent Roles of p75 NTR and Trk Receptors in BDNF's Effects on Dendritic Spine Density and Morphology"

    Article Title: Divergent Roles of p75 NTR and Trk Receptors in BDNF's Effects on Dendritic Spine Density and Morphology

    Journal: Neural Plasticity

    doi: 10.1155/2012/578057

    Role of Trk and p75 NTR in BDNF's effects on dendritic spine density and morphology. (a) Representative examples of dendritic segments of CA1 pyramidal neurons maintained in serum-containing media (SM) and treated with the function-blocking antibody of p75 NTR , REX (50 μ g/mL), and BDNF (250 ng/mL) for 48 hrs (scale bar represents 2 μ m). (b) Dendritic spine density expressed per 10 μ m of apical dendrite. (c) Proportion of each morphological type of dendritic spine, expressed as a fraction of the total spine population. (d) Representative examples of dendritic segments of CA1 pyramidal neurons maintained in SM and treated with k-252a (200 nM) and BDNF (250 ng/mL) for 48 hrs. (e) Dendritic spine density expressed per 10 μ m of apical dendrite. (f) Proportion of each morphological type of dendritic spines, expressed as a fraction of the total spine population. * P < 0.05, ** P < 0.01, and *** P < 0.001, after a one-way ANOVA.
    Figure Legend Snippet: Role of Trk and p75 NTR in BDNF's effects on dendritic spine density and morphology. (a) Representative examples of dendritic segments of CA1 pyramidal neurons maintained in serum-containing media (SM) and treated with the function-blocking antibody of p75 NTR , REX (50 μ g/mL), and BDNF (250 ng/mL) for 48 hrs (scale bar represents 2 μ m). (b) Dendritic spine density expressed per 10 μ m of apical dendrite. (c) Proportion of each morphological type of dendritic spine, expressed as a fraction of the total spine population. (d) Representative examples of dendritic segments of CA1 pyramidal neurons maintained in SM and treated with k-252a (200 nM) and BDNF (250 ng/mL) for 48 hrs. (e) Dendritic spine density expressed per 10 μ m of apical dendrite. (f) Proportion of each morphological type of dendritic spines, expressed as a fraction of the total spine population. * P < 0.05, ** P < 0.01, and *** P < 0.001, after a one-way ANOVA.

    Techniques Used: Blocking Assay

    Role of neuronal activity in k-252a's effects on dendritic spine density and morphology. (a) Representative examples of dendritic segments of CA1 pyramidal neurons maintained in serum-containing media (SM) and treated with k-252a (200 nM), TTX (1 μ M), or both k-252a and TTX for 48 hrs (scale bar represents 2 μ m). (b) Dendritic spine density expressed per 10 μ m of apical dendrite. (c) Proportion of each morphological type of dendritic spines, expressed as a fraction of the total spine population. * P < 0.05, ** P < 0.01, and *** P < 0.001, after a one-way ANOVA.
    Figure Legend Snippet: Role of neuronal activity in k-252a's effects on dendritic spine density and morphology. (a) Representative examples of dendritic segments of CA1 pyramidal neurons maintained in serum-containing media (SM) and treated with k-252a (200 nM), TTX (1 μ M), or both k-252a and TTX for 48 hrs (scale bar represents 2 μ m). (b) Dendritic spine density expressed per 10 μ m of apical dendrite. (c) Proportion of each morphological type of dendritic spines, expressed as a fraction of the total spine population. * P < 0.05, ** P < 0.01, and *** P < 0.001, after a one-way ANOVA.

    Techniques Used: Activity Assay

    Quantitative results of dendritic spine analyses .
    Figure Legend Snippet: Quantitative results of dendritic spine analyses .

    Techniques Used:

    Summary of total dendritic length, cells, slices, and individual spines sampled for the quantitative dendritic spine analyses.
    Figure Legend Snippet: Summary of total dendritic length, cells, slices, and individual spines sampled for the quantitative dendritic spine analyses.

    Techniques Used:

    k252a  (Alomone Labs)


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    Alomone Labs k252a
    (A) CGNs immunostained for Sig-1R (red) and TrkB (green). Scale Bar: 10 µm. (B and C) CGNs were cultured for 24 h with or without PRE-084. As for cultures incubated with <t>K252a,</t> a pan-tropomyosin receptor kinase (trk) K252a was added at the same time as PRE was added to the culture. Representative images of CGNs are shown in (B). The neurite lengths were quantified from three independent experiments. The mean lengths of neurite are shown in the graph (C). Scale bar: 20 µm. (D) The same effects were observed when the cells were cultured in the presence of BDNF; n = 3, ** P <0.01, Scheffe’s test.
    K252a, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Images

    1) Product Images from "Sigma-1 Receptor Enhances Neurite Elongation of Cerebellar Granule Neurons via TrkB Signaling"

    Article Title: Sigma-1 Receptor Enhances Neurite Elongation of Cerebellar Granule Neurons via TrkB Signaling

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0075760

    (A) CGNs immunostained for Sig-1R (red) and TrkB (green). Scale Bar: 10 µm. (B and C) CGNs were cultured for 24 h with or without PRE-084. As for cultures incubated with K252a, a pan-tropomyosin receptor kinase (trk) K252a was added at the same time as PRE was added to the culture. Representative images of CGNs are shown in (B). The neurite lengths were quantified from three independent experiments. The mean lengths of neurite are shown in the graph (C). Scale bar: 20 µm. (D) The same effects were observed when the cells were cultured in the presence of BDNF; n = 3, ** P <0.01, Scheffe’s test.
    Figure Legend Snippet: (A) CGNs immunostained for Sig-1R (red) and TrkB (green). Scale Bar: 10 µm. (B and C) CGNs were cultured for 24 h with or without PRE-084. As for cultures incubated with K252a, a pan-tropomyosin receptor kinase (trk) K252a was added at the same time as PRE was added to the culture. Representative images of CGNs are shown in (B). The neurite lengths were quantified from three independent experiments. The mean lengths of neurite are shown in the graph (C). Scale bar: 20 µm. (D) The same effects were observed when the cells were cultured in the presence of BDNF; n = 3, ** P <0.01, Scheffe’s test.

    Techniques Used: Cell Culture, Incubation

    252a  (Alomone Labs)


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    Alomone Labs 252a
    252a, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    k 252a  (Alomone Labs)


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    Alomone Labs k 252a
    K 252a, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    k252a  (Alomone Labs)


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    Alomone Labs k252a
    <t>K252a</t> decreased the antidepressant action of Rh2 in chronic unpredictable mild stress (CUMS)‐stressed mice. Co‐treatment with (K252a + Rh2) of the mice against CUMS showed the longer immobility time than the (CUMS + Rh2)‐treated mice in the forced swim test (FST)(a) and tail suspension test (TST) (b). K252a blocked the reversing effects of Rh2 on sucrose consumption of the CUMS‐treated mice (c). All data are expressed as the means ± SEM, n = 10. ** p < .01 when compared to the Vehicle; ## p < .01 when compared to the CUMS. The comparisons were made by two‐way analysis of variance (ANOVA) followed by Bonferroni's test
    K252a, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    1) Product Images from "Ginsenoside Rh2 administration produces crucial antidepressant‐like effects in a CUMS‐induced mice model of depression"

    Article Title: Ginsenoside Rh2 administration produces crucial antidepressant‐like effects in a CUMS‐induced mice model of depression

    Journal: Brain and Behavior

    doi: 10.1002/brb3.2705

    K252a decreased the antidepressant action of Rh2 in chronic unpredictable mild stress (CUMS)‐stressed mice. Co‐treatment with (K252a + Rh2) of the mice against CUMS showed the longer immobility time than the (CUMS + Rh2)‐treated mice in the forced swim test (FST)(a) and tail suspension test (TST) (b). K252a blocked the reversing effects of Rh2 on sucrose consumption of the CUMS‐treated mice (c). All data are expressed as the means ± SEM, n = 10. ** p < .01 when compared to the Vehicle; ## p < .01 when compared to the CUMS. The comparisons were made by two‐way analysis of variance (ANOVA) followed by Bonferroni's test
    Figure Legend Snippet: K252a decreased the antidepressant action of Rh2 in chronic unpredictable mild stress (CUMS)‐stressed mice. Co‐treatment with (K252a + Rh2) of the mice against CUMS showed the longer immobility time than the (CUMS + Rh2)‐treated mice in the forced swim test (FST)(a) and tail suspension test (TST) (b). K252a blocked the reversing effects of Rh2 on sucrose consumption of the CUMS‐treated mice (c). All data are expressed as the means ± SEM, n = 10. ** p < .01 when compared to the Vehicle; ## p < .01 when compared to the CUMS. The comparisons were made by two‐way analysis of variance (ANOVA) followed by Bonferroni's test

    Techniques Used:

    The enhancing influence of Rh2 on the expression of BDNF‐CREB signaling pathway was blocked by the inhibitor K252a in mice exposed to chronic unpredictable mild stress (CUMS). BDNF, pERK/ERK, pAKT/AKT, and pCREB/CREB protein levels were lower in (CUMS + Rh2 + K252a)‐treated mice than the (CUMS + Rh2)‐treated mice. The obtained data are expressed as the means ± SEM, n = 5. ** p < .01 when compared to vehicle; ## p < .01 when compared to CUMS. The comparisons were made by two‐way analysis of variance (ANOVA) followed by Bonferroni's test
    Figure Legend Snippet: The enhancing influence of Rh2 on the expression of BDNF‐CREB signaling pathway was blocked by the inhibitor K252a in mice exposed to chronic unpredictable mild stress (CUMS). BDNF, pERK/ERK, pAKT/AKT, and pCREB/CREB protein levels were lower in (CUMS + Rh2 + K252a)‐treated mice than the (CUMS + Rh2)‐treated mice. The obtained data are expressed as the means ± SEM, n = 5. ** p < .01 when compared to vehicle; ## p < .01 when compared to CUMS. The comparisons were made by two‐way analysis of variance (ANOVA) followed by Bonferroni's test

    Techniques Used: Expressing

    K252a blocked the enhancing effects of Rh2 on neurogenesis. Compared to the (chronic unpredictable mild stress [CUMS] + Rh2)‐treated group, the increase of DCX + neurons in the DG of mice exposed to CUMS were significantly blocked by K252a. Scale bar: 100 μm. The results of analysis are expressed as the means ± SEM, n = 5. ** p < .01 when compared to vehicle; ## p < .01 when compared to CUMS. The comparisons were made by two‐way analysis of variance (ANOVA) followed by Bonferroni's test
    Figure Legend Snippet: K252a blocked the enhancing effects of Rh2 on neurogenesis. Compared to the (chronic unpredictable mild stress [CUMS] + Rh2)‐treated group, the increase of DCX + neurons in the DG of mice exposed to CUMS were significantly blocked by K252a. Scale bar: 100 μm. The results of analysis are expressed as the means ± SEM, n = 5. ** p < .01 when compared to vehicle; ## p < .01 when compared to CUMS. The comparisons were made by two‐way analysis of variance (ANOVA) followed by Bonferroni's test

    Techniques Used:

    k 252a  (Alomone Labs)


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    Alomone Labs k 252a
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    Alomone Labs k252a
    ( A ) Validation of the cdk1 sandwich ELISA assay performed with 10 fg of either GST (GST) or a chimeric protein containing the sequence of human cdk1 bound to GST (Cdk1-GST), and revealed with the anti-cdk1 PSTAIRE antibody. Normalized optical density (O.D.) at 405 nm is shown. ( B ) Validation of the phospho-cdk1 sandwich ELISA assay performed with cell lysates obtained from DCRNs cultured in the presence of 100 ng/ml NGF and 2 ng/ml BDNF, incubated either in the absence (- CIP) or presence (+ CIP) of CIP, and revealed with the anti-cdk1 (pTyr15) antibody. Normalized optical density (O.D.) at 405 nm is shown. ( C ) Cell lysates from DCRNs cultured in the presence (+) or absence (-) of the referred factors were subjected to sandwich ELISA analyses using antibodies specific for either cdk1 phosphorylated at Tyr15 or total cdk1 protein. Colorimetric values for cdk1 phosphorylated at Tyr15 were normalized to the levels of cdk1 protein (Cdk1). ( D ) Cell lysates from DCRNs cultured in the presence of the referred factors and/or <t>K252a</t> were subjected to sandwich ELISA analyses as described above. Colorimetric values for cdk1 phosphorylated at Tyr15 were normalized to the levels of cdk1 protein (Cdk1). *p<0.05; ***p<0.005 (Student’s t test; n = 3).
    K252a, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Alomone Labs k 252a
    Role of Trk and p75 NTR in BDNF's effects on dendritic spine density and morphology. (a) Representative examples of dendritic segments of CA1 pyramidal neurons maintained in serum-containing media (SM) and treated with the function-blocking antibody of p75 NTR , REX (50 μ g/mL), and BDNF (250 ng/mL) for 48 hrs (scale bar represents 2 μ m). (b) Dendritic spine density expressed per 10 μ m of apical dendrite. (c) Proportion of each morphological type of dendritic spine, expressed as a fraction of the total spine population. (d) Representative examples of dendritic segments of CA1 pyramidal neurons maintained in SM and treated with <t>k-252a</t> (200 nM) and BDNF (250 ng/mL) for 48 hrs. (e) Dendritic spine density expressed per 10 μ m of apical dendrite. (f) Proportion of each morphological type of dendritic spines, expressed as a fraction of the total spine population. * P < 0.05, ** P < 0.01, and *** P < 0.001, after a one-way ANOVA.
    K 252a, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Alomone Labs 252a
    Role of Trk and p75 NTR in BDNF's effects on dendritic spine density and morphology. (a) Representative examples of dendritic segments of CA1 pyramidal neurons maintained in serum-containing media (SM) and treated with the function-blocking antibody of p75 NTR , REX (50 μ g/mL), and BDNF (250 ng/mL) for 48 hrs (scale bar represents 2 μ m). (b) Dendritic spine density expressed per 10 μ m of apical dendrite. (c) Proportion of each morphological type of dendritic spine, expressed as a fraction of the total spine population. (d) Representative examples of dendritic segments of CA1 pyramidal neurons maintained in SM and treated with <t>k-252a</t> (200 nM) and BDNF (250 ng/mL) for 48 hrs. (e) Dendritic spine density expressed per 10 μ m of apical dendrite. (f) Proportion of each morphological type of dendritic spines, expressed as a fraction of the total spine population. * P < 0.05, ** P < 0.01, and *** P < 0.001, after a one-way ANOVA.
    252a, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    ( A ) Validation of the cdk1 sandwich ELISA assay performed with 10 fg of either GST (GST) or a chimeric protein containing the sequence of human cdk1 bound to GST (Cdk1-GST), and revealed with the anti-cdk1 PSTAIRE antibody. Normalized optical density (O.D.) at 405 nm is shown. ( B ) Validation of the phospho-cdk1 sandwich ELISA assay performed with cell lysates obtained from DCRNs cultured in the presence of 100 ng/ml NGF and 2 ng/ml BDNF, incubated either in the absence (- CIP) or presence (+ CIP) of CIP, and revealed with the anti-cdk1 (pTyr15) antibody. Normalized optical density (O.D.) at 405 nm is shown. ( C ) Cell lysates from DCRNs cultured in the presence (+) or absence (-) of the referred factors were subjected to sandwich ELISA analyses using antibodies specific for either cdk1 phosphorylated at Tyr15 or total cdk1 protein. Colorimetric values for cdk1 phosphorylated at Tyr15 were normalized to the levels of cdk1 protein (Cdk1). ( D ) Cell lysates from DCRNs cultured in the presence of the referred factors and/or K252a were subjected to sandwich ELISA analyses as described above. Colorimetric values for cdk1 phosphorylated at Tyr15 were normalized to the levels of cdk1 protein (Cdk1). *p<0.05; ***p<0.005 (Student’s t test; n = 3).

    Journal: PLoS ONE

    Article Title: Brain-Derived Neurotrophic Factor-Dependent cdk1 Inhibition Prevents G2/M Progression in Differentiating Tetraploid Neurons

    doi: 10.1371/journal.pone.0064890

    Figure Lengend Snippet: ( A ) Validation of the cdk1 sandwich ELISA assay performed with 10 fg of either GST (GST) or a chimeric protein containing the sequence of human cdk1 bound to GST (Cdk1-GST), and revealed with the anti-cdk1 PSTAIRE antibody. Normalized optical density (O.D.) at 405 nm is shown. ( B ) Validation of the phospho-cdk1 sandwich ELISA assay performed with cell lysates obtained from DCRNs cultured in the presence of 100 ng/ml NGF and 2 ng/ml BDNF, incubated either in the absence (- CIP) or presence (+ CIP) of CIP, and revealed with the anti-cdk1 (pTyr15) antibody. Normalized optical density (O.D.) at 405 nm is shown. ( C ) Cell lysates from DCRNs cultured in the presence (+) or absence (-) of the referred factors were subjected to sandwich ELISA analyses using antibodies specific for either cdk1 phosphorylated at Tyr15 or total cdk1 protein. Colorimetric values for cdk1 phosphorylated at Tyr15 were normalized to the levels of cdk1 protein (Cdk1). ( D ) Cell lysates from DCRNs cultured in the presence of the referred factors and/or K252a were subjected to sandwich ELISA analyses as described above. Colorimetric values for cdk1 phosphorylated at Tyr15 were normalized to the levels of cdk1 protein (Cdk1). *p<0.05; ***p<0.005 (Student’s t test; n = 3).

    Article Snippet: K252a (200 nM) (Alomone Labs) and MK-1775 (300 nM) (Axon MedChem), both prepared in dimethyl sulfoxide, were added to the culture medium 10 min before BDNF treatment (DCRNs).

    Techniques: Sandwich ELISA, Sequencing, Cell Culture, Incubation

    Role of Trk and p75 NTR in BDNF's effects on dendritic spine density and morphology. (a) Representative examples of dendritic segments of CA1 pyramidal neurons maintained in serum-containing media (SM) and treated with the function-blocking antibody of p75 NTR , REX (50 μ g/mL), and BDNF (250 ng/mL) for 48 hrs (scale bar represents 2 μ m). (b) Dendritic spine density expressed per 10 μ m of apical dendrite. (c) Proportion of each morphological type of dendritic spine, expressed as a fraction of the total spine population. (d) Representative examples of dendritic segments of CA1 pyramidal neurons maintained in SM and treated with k-252a (200 nM) and BDNF (250 ng/mL) for 48 hrs. (e) Dendritic spine density expressed per 10 μ m of apical dendrite. (f) Proportion of each morphological type of dendritic spines, expressed as a fraction of the total spine population. * P < 0.05, ** P < 0.01, and *** P < 0.001, after a one-way ANOVA.

    Journal: Neural Plasticity

    Article Title: Divergent Roles of p75 NTR and Trk Receptors in BDNF's Effects on Dendritic Spine Density and Morphology

    doi: 10.1155/2012/578057

    Figure Lengend Snippet: Role of Trk and p75 NTR in BDNF's effects on dendritic spine density and morphology. (a) Representative examples of dendritic segments of CA1 pyramidal neurons maintained in serum-containing media (SM) and treated with the function-blocking antibody of p75 NTR , REX (50 μ g/mL), and BDNF (250 ng/mL) for 48 hrs (scale bar represents 2 μ m). (b) Dendritic spine density expressed per 10 μ m of apical dendrite. (c) Proportion of each morphological type of dendritic spine, expressed as a fraction of the total spine population. (d) Representative examples of dendritic segments of CA1 pyramidal neurons maintained in SM and treated with k-252a (200 nM) and BDNF (250 ng/mL) for 48 hrs. (e) Dendritic spine density expressed per 10 μ m of apical dendrite. (f) Proportion of each morphological type of dendritic spines, expressed as a fraction of the total spine population. * P < 0.05, ** P < 0.01, and *** P < 0.001, after a one-way ANOVA.

    Article Snippet: Slices were kept in a serum containing media throughout the course of the experiments and were randomly assigned to the following groups: (1) control, serum-containing media; (2) BDNF (250 ng/mL); (3) anti-p75 NTR antibody REX (50 μ g/mL), known to block p75 NTR function [ ] (provided by L. Reichardt, UCSF); (4) BDNF in the presence of REX antibody; (5) k-252a (200 nM, in DMSO; Calbiochem; San Diego, CA) to block autophosphorylation and activation of tyrosine kinase domains of plasma membrane neurotrophin receptors [ ]; (6) BDNF in the presence of k-252a; (7) TTX (1 μ M; Alomone Labs; Jerusalem, Israel); (8) TTX in the presence of k-252a.

    Techniques: Blocking Assay

    Role of neuronal activity in k-252a's effects on dendritic spine density and morphology. (a) Representative examples of dendritic segments of CA1 pyramidal neurons maintained in serum-containing media (SM) and treated with k-252a (200 nM), TTX (1 μ M), or both k-252a and TTX for 48 hrs (scale bar represents 2 μ m). (b) Dendritic spine density expressed per 10 μ m of apical dendrite. (c) Proportion of each morphological type of dendritic spines, expressed as a fraction of the total spine population. * P < 0.05, ** P < 0.01, and *** P < 0.001, after a one-way ANOVA.

    Journal: Neural Plasticity

    Article Title: Divergent Roles of p75 NTR and Trk Receptors in BDNF's Effects on Dendritic Spine Density and Morphology

    doi: 10.1155/2012/578057

    Figure Lengend Snippet: Role of neuronal activity in k-252a's effects on dendritic spine density and morphology. (a) Representative examples of dendritic segments of CA1 pyramidal neurons maintained in serum-containing media (SM) and treated with k-252a (200 nM), TTX (1 μ M), or both k-252a and TTX for 48 hrs (scale bar represents 2 μ m). (b) Dendritic spine density expressed per 10 μ m of apical dendrite. (c) Proportion of each morphological type of dendritic spines, expressed as a fraction of the total spine population. * P < 0.05, ** P < 0.01, and *** P < 0.001, after a one-way ANOVA.

    Article Snippet: Slices were kept in a serum containing media throughout the course of the experiments and were randomly assigned to the following groups: (1) control, serum-containing media; (2) BDNF (250 ng/mL); (3) anti-p75 NTR antibody REX (50 μ g/mL), known to block p75 NTR function [ ] (provided by L. Reichardt, UCSF); (4) BDNF in the presence of REX antibody; (5) k-252a (200 nM, in DMSO; Calbiochem; San Diego, CA) to block autophosphorylation and activation of tyrosine kinase domains of plasma membrane neurotrophin receptors [ ]; (6) BDNF in the presence of k-252a; (7) TTX (1 μ M; Alomone Labs; Jerusalem, Israel); (8) TTX in the presence of k-252a.

    Techniques: Activity Assay

    Quantitative results of dendritic spine analyses .

    Journal: Neural Plasticity

    Article Title: Divergent Roles of p75 NTR and Trk Receptors in BDNF's Effects on Dendritic Spine Density and Morphology

    doi: 10.1155/2012/578057

    Figure Lengend Snippet: Quantitative results of dendritic spine analyses .

    Article Snippet: Slices were kept in a serum containing media throughout the course of the experiments and were randomly assigned to the following groups: (1) control, serum-containing media; (2) BDNF (250 ng/mL); (3) anti-p75 NTR antibody REX (50 μ g/mL), known to block p75 NTR function [ ] (provided by L. Reichardt, UCSF); (4) BDNF in the presence of REX antibody; (5) k-252a (200 nM, in DMSO; Calbiochem; San Diego, CA) to block autophosphorylation and activation of tyrosine kinase domains of plasma membrane neurotrophin receptors [ ]; (6) BDNF in the presence of k-252a; (7) TTX (1 μ M; Alomone Labs; Jerusalem, Israel); (8) TTX in the presence of k-252a.

    Techniques:

    Summary of total dendritic length, cells, slices, and individual spines sampled for the quantitative dendritic spine analyses.

    Journal: Neural Plasticity

    Article Title: Divergent Roles of p75 NTR and Trk Receptors in BDNF's Effects on Dendritic Spine Density and Morphology

    doi: 10.1155/2012/578057

    Figure Lengend Snippet: Summary of total dendritic length, cells, slices, and individual spines sampled for the quantitative dendritic spine analyses.

    Article Snippet: Slices were kept in a serum containing media throughout the course of the experiments and were randomly assigned to the following groups: (1) control, serum-containing media; (2) BDNF (250 ng/mL); (3) anti-p75 NTR antibody REX (50 μ g/mL), known to block p75 NTR function [ ] (provided by L. Reichardt, UCSF); (4) BDNF in the presence of REX antibody; (5) k-252a (200 nM, in DMSO; Calbiochem; San Diego, CA) to block autophosphorylation and activation of tyrosine kinase domains of plasma membrane neurotrophin receptors [ ]; (6) BDNF in the presence of k-252a; (7) TTX (1 μ M; Alomone Labs; Jerusalem, Israel); (8) TTX in the presence of k-252a.

    Techniques: