acsf  (Alomone Labs)


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    Structured Review

    Alomone Labs acsf
    Acsf, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/acsf/product/Alomone Labs
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    acsf - by Bioz Stars, 2022-12
    93/100 stars

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    Alomone Labs rabbit anti twik 1
    Cellular identification of GFP-expressing cells of the DG, LEC, and Cb in P56 of <t>TWIK-1</t> BAC-GFP Tg mice. ( A ) Overview of GFP expression in DG, LEC, and CB of TWIK-1 BAC-GFP Tg mice. Scale bar, 200 μm. ( B ) Representative co-immunofluorescence images with GFP, NeuN, and GAD67 antibodies. Scale bar, 200 μm. ( C ) Quantification bar graph of the cell type of GFP-positive cells in each brain area from B. Quantification was analyzed by the percentage of each cell type from all GFP-positive cells. Raw data are listed in Supplementary Materials Table S1 . Data are presented as the Mean ± SEM.
    Rabbit Anti Twik 1, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rabbit anti twik 1/product/Alomone Labs
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    rabbit anti twik 1 - by Bioz Stars, 2022-12
    93/100 stars
      Buy from Supplier

    93
    Alomone Labs kynurenic acid
    Cellular identification of GFP-expressing cells of the DG, LEC, and Cb in P56 of <t>TWIK-1</t> BAC-GFP Tg mice. ( A ) Overview of GFP expression in DG, LEC, and CB of TWIK-1 BAC-GFP Tg mice. Scale bar, 200 μm. ( B ) Representative co-immunofluorescence images with GFP, NeuN, and GAD67 antibodies. Scale bar, 200 μm. ( C ) Quantification bar graph of the cell type of GFP-positive cells in each brain area from B. Quantification was analyzed by the percentage of each cell type from all GFP-positive cells. Raw data are listed in Supplementary Materials Table S1 . Data are presented as the Mean ± SEM.
    Kynurenic Acid, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/kynurenic acid/product/Alomone Labs
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    kynurenic acid - by Bioz Stars, 2022-12
    93/100 stars
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    Image Search Results


    Cellular identification of GFP-expressing cells of the DG, LEC, and Cb in P56 of TWIK-1 BAC-GFP Tg mice. ( A ) Overview of GFP expression in DG, LEC, and CB of TWIK-1 BAC-GFP Tg mice. Scale bar, 200 μm. ( B ) Representative co-immunofluorescence images with GFP, NeuN, and GAD67 antibodies. Scale bar, 200 μm. ( C ) Quantification bar graph of the cell type of GFP-positive cells in each brain area from B. Quantification was analyzed by the percentage of each cell type from all GFP-positive cells. Raw data are listed in Supplementary Materials Table S1 . Data are presented as the Mean ± SEM.

    Journal: Cells

    Article Title: TWIK-1 BAC-GFP Transgenic Mice, an Animal Model for TWIK-1 Expression

    doi: 10.3390/cells10102751

    Figure Lengend Snippet: Cellular identification of GFP-expressing cells of the DG, LEC, and Cb in P56 of TWIK-1 BAC-GFP Tg mice. ( A ) Overview of GFP expression in DG, LEC, and CB of TWIK-1 BAC-GFP Tg mice. Scale bar, 200 μm. ( B ) Representative co-immunofluorescence images with GFP, NeuN, and GAD67 antibodies. Scale bar, 200 μm. ( C ) Quantification bar graph of the cell type of GFP-positive cells in each brain area from B. Quantification was analyzed by the percentage of each cell type from all GFP-positive cells. Raw data are listed in Supplementary Materials Table S1 . Data are presented as the Mean ± SEM.

    Article Snippet: The following antibodies were used: chicken anti-GFP (Abcam, Cat#; ab136970, 1:300); rabbit anti-TWIK-1 (Alomone labs, Cat#; APC-10, 1:200); mouse anti-NeuN (Abcam, Cat#; ab104224, 1:100); rabbit anti-GAD67 (GeneTex, Cat#; GTX113190, 1:300); guinea pig anti-doublecortin (Millipore, Cat#; AB2253, 1:100); rabbit anti-calbindin (Swant, Cat#; CB38, 1:2000); rat anti-Ki67 (Invitrogen, Cat#; 14-5698-85, 1:200); rat anti-GFAP (Invitrogen, Cat#; 13-0300, 1:500); rabbit anti-NG2 (Millipore, Cat#; AB5320, 1:300); and Alexa Fluor 488-, 594-, and 647-conjugated secondary antibodies (Jackson ImmunoResearch, 1:300).

    Techniques: Expressing, BAC Assay, Mouse Assay, Immunofluorescence

    Generation of TWIK-1 BAC-GFP Tg mice. ( A ) Schematic design of the modified Kcnk1 BAC vector. GFP coding sequence was inserted just before the start codon of Kcnk1 . ( B ) Genotype PCR results for the designed primer pair in A. ( C ) Representative macroscopic GFP fluorescent images in the adult brain (P56 age) from TWIK-1 BAC-GFP Tg mice. Upper left numbers indicate the relative position of the brain slice from bregma. Strong GFP expression was identified in DG, LEC, and Cb (yellow dashed arrow), Scale bar, 1000 μm. ( D ) Representative co-immunofluorescence images with GFP and TWIK-1 in the DG. Scale bar, 200 μm. ( E ) Enlarged inset from D. Scale bar, 50 μm. ( F ) Representative co-immunofluorescence images with GFP and TWIK-1 in the LEC. Scale bar, 200 μm. ( G ) Enlarged inset from F. Scale bar, 50 μm. ( H ) Representative co-immunofluorescence images with GFP and TWIK-1 in the Cb. Scale bar, 200 μm. ( I ) Enlarged inset from H. Scale bar, 50 μm.

    Journal: Cells

    Article Title: TWIK-1 BAC-GFP Transgenic Mice, an Animal Model for TWIK-1 Expression

    doi: 10.3390/cells10102751

    Figure Lengend Snippet: Generation of TWIK-1 BAC-GFP Tg mice. ( A ) Schematic design of the modified Kcnk1 BAC vector. GFP coding sequence was inserted just before the start codon of Kcnk1 . ( B ) Genotype PCR results for the designed primer pair in A. ( C ) Representative macroscopic GFP fluorescent images in the adult brain (P56 age) from TWIK-1 BAC-GFP Tg mice. Upper left numbers indicate the relative position of the brain slice from bregma. Strong GFP expression was identified in DG, LEC, and Cb (yellow dashed arrow), Scale bar, 1000 μm. ( D ) Representative co-immunofluorescence images with GFP and TWIK-1 in the DG. Scale bar, 200 μm. ( E ) Enlarged inset from D. Scale bar, 50 μm. ( F ) Representative co-immunofluorescence images with GFP and TWIK-1 in the LEC. Scale bar, 200 μm. ( G ) Enlarged inset from F. Scale bar, 50 μm. ( H ) Representative co-immunofluorescence images with GFP and TWIK-1 in the Cb. Scale bar, 200 μm. ( I ) Enlarged inset from H. Scale bar, 50 μm.

    Article Snippet: The following antibodies were used: chicken anti-GFP (Abcam, Cat#; ab136970, 1:300); rabbit anti-TWIK-1 (Alomone labs, Cat#; APC-10, 1:200); mouse anti-NeuN (Abcam, Cat#; ab104224, 1:100); rabbit anti-GAD67 (GeneTex, Cat#; GTX113190, 1:300); guinea pig anti-doublecortin (Millipore, Cat#; AB2253, 1:100); rabbit anti-calbindin (Swant, Cat#; CB38, 1:2000); rat anti-Ki67 (Invitrogen, Cat#; 14-5698-85, 1:200); rat anti-GFAP (Invitrogen, Cat#; 13-0300, 1:500); rabbit anti-NG2 (Millipore, Cat#; AB5320, 1:300); and Alexa Fluor 488-, 594-, and 647-conjugated secondary antibodies (Jackson ImmunoResearch, 1:300).

    Techniques: BAC Assay, Mouse Assay, Modification, Plasmid Preparation, Sequencing, Polymerase Chain Reaction, Slice Preparation, Expressing, Immunofluorescence

    Glial expression of TWIK-1 in TWIK-1 BAC-GFP Tg mice. ( A ) Representative co-immunofluorescence images with TWIK-1 and GFAP. Scale bar, 200 μm. ( B ) Enlarged inset from A. Yellow arrow indicates double immunoreactive cells with TWIK-1 and GFAP. Scale bar, 50 μm. ( C ) Representative co-immunofluorescence images with GFP and GFAP. Scale bar, 200 μm. ( D ) Enlarged inset from C. Yellow arrow indicates double immunoreactive cells with GFP and GFAP. Scale bar, 50 μm. ( E ) Representative co-immunofluorescence images with GFP and Iba1. Scale bar, 200 μm. ( F ) Enlarged inset from E. There are no Iba1-positive glial-like GFP-expressing cells (White arrow). Scale bar, 50 μm. ( G ) Representative co-immunofluorescence images with GFP and NG2. Scale bar, 200 μm. ( H ) Enlarged inset from G. There are no NG2-positive glial-like GFP-expressing cells (White arrow). Scale bar, 50 μm.

    Journal: Cells

    Article Title: TWIK-1 BAC-GFP Transgenic Mice, an Animal Model for TWIK-1 Expression

    doi: 10.3390/cells10102751

    Figure Lengend Snippet: Glial expression of TWIK-1 in TWIK-1 BAC-GFP Tg mice. ( A ) Representative co-immunofluorescence images with TWIK-1 and GFAP. Scale bar, 200 μm. ( B ) Enlarged inset from A. Yellow arrow indicates double immunoreactive cells with TWIK-1 and GFAP. Scale bar, 50 μm. ( C ) Representative co-immunofluorescence images with GFP and GFAP. Scale bar, 200 μm. ( D ) Enlarged inset from C. Yellow arrow indicates double immunoreactive cells with GFP and GFAP. Scale bar, 50 μm. ( E ) Representative co-immunofluorescence images with GFP and Iba1. Scale bar, 200 μm. ( F ) Enlarged inset from E. There are no Iba1-positive glial-like GFP-expressing cells (White arrow). Scale bar, 50 μm. ( G ) Representative co-immunofluorescence images with GFP and NG2. Scale bar, 200 μm. ( H ) Enlarged inset from G. There are no NG2-positive glial-like GFP-expressing cells (White arrow). Scale bar, 50 μm.

    Article Snippet: The following antibodies were used: chicken anti-GFP (Abcam, Cat#; ab136970, 1:300); rabbit anti-TWIK-1 (Alomone labs, Cat#; APC-10, 1:200); mouse anti-NeuN (Abcam, Cat#; ab104224, 1:100); rabbit anti-GAD67 (GeneTex, Cat#; GTX113190, 1:300); guinea pig anti-doublecortin (Millipore, Cat#; AB2253, 1:100); rabbit anti-calbindin (Swant, Cat#; CB38, 1:2000); rat anti-Ki67 (Invitrogen, Cat#; 14-5698-85, 1:200); rat anti-GFAP (Invitrogen, Cat#; 13-0300, 1:500); rabbit anti-NG2 (Millipore, Cat#; AB5320, 1:300); and Alexa Fluor 488-, 594-, and 647-conjugated secondary antibodies (Jackson ImmunoResearch, 1:300).

    Techniques: Expressing, BAC Assay, Mouse Assay, Immunofluorescence

    High TWIK-1 expression in immature neurons of the DG in TWIK-1 BAC-GFP Tg mice. ( A ) Representative co-immunofluorescence images with GFP, DCX, CB, and Ki67 in P56 of DG. Scale bar, 200 μm. ( B ) Enlarged inset from A. Most of strong GFP-expressing cells co-labeled with DCX (yellow arrow), but not with CB and Ki67 (white arrow). Scale bar, 10 μm. ( C , D ) Quantification of the cell type of strong GFP-expressing cells. Raw data are listed in Supplementary Materials Table S1 . Data are presented as the Mean ± SEM.

    Journal: Cells

    Article Title: TWIK-1 BAC-GFP Transgenic Mice, an Animal Model for TWIK-1 Expression

    doi: 10.3390/cells10102751

    Figure Lengend Snippet: High TWIK-1 expression in immature neurons of the DG in TWIK-1 BAC-GFP Tg mice. ( A ) Representative co-immunofluorescence images with GFP, DCX, CB, and Ki67 in P56 of DG. Scale bar, 200 μm. ( B ) Enlarged inset from A. Most of strong GFP-expressing cells co-labeled with DCX (yellow arrow), but not with CB and Ki67 (white arrow). Scale bar, 10 μm. ( C , D ) Quantification of the cell type of strong GFP-expressing cells. Raw data are listed in Supplementary Materials Table S1 . Data are presented as the Mean ± SEM.

    Article Snippet: The following antibodies were used: chicken anti-GFP (Abcam, Cat#; ab136970, 1:300); rabbit anti-TWIK-1 (Alomone labs, Cat#; APC-10, 1:200); mouse anti-NeuN (Abcam, Cat#; ab104224, 1:100); rabbit anti-GAD67 (GeneTex, Cat#; GTX113190, 1:300); guinea pig anti-doublecortin (Millipore, Cat#; AB2253, 1:100); rabbit anti-calbindin (Swant, Cat#; CB38, 1:2000); rat anti-Ki67 (Invitrogen, Cat#; 14-5698-85, 1:200); rat anti-GFAP (Invitrogen, Cat#; 13-0300, 1:500); rabbit anti-NG2 (Millipore, Cat#; AB5320, 1:300); and Alexa Fluor 488-, 594-, and 647-conjugated secondary antibodies (Jackson ImmunoResearch, 1:300).

    Techniques: Expressing, BAC Assay, Mouse Assay, Immunofluorescence, Labeling

    TWIK-1 BAC-GFP Tg mice represent kainic acid (KA)-induced increase of TWIK-1 expression. ( A ) Representative GFP expression in a brain slice from saline or KA-treated TWIK-1 BAC-GFP Tg mice. Scale bar, 1000 μm. ( B ) Representative GFP expression in the DG from saline or KA-treated TWIK-1 BAC-GFP Tg mice. Scale bar, 200 μm. Enlarged inset from the experiment. Scale bar, 50 μm. ( C ) Quantification of relative mean GFP intensity of the granule cell layer. ( D ) Representative Kcnk1 mRNA fluorescence in situ hybridization (FISH) images of DG from saline- or KA-treated mice. Scale bar, 100 μm. ( E ) Quantification bar graph of Kcnk1 mRNA spot density in the granule cell layer from saline- or KA-treated mice. ( F ) Representative TWIK-1 immunofluorescence images of DG from saline- or KA-treated mice. Scale bar, 200 μm. ( G ) Quantification bar graph of the relative mean TWIK-1 immunofluorescence in the granule cell layer of saline- or KA-treated mice. Raw data are listed in Supplementary Materials Table S1 . **** p

    Journal: Cells

    Article Title: TWIK-1 BAC-GFP Transgenic Mice, an Animal Model for TWIK-1 Expression

    doi: 10.3390/cells10102751

    Figure Lengend Snippet: TWIK-1 BAC-GFP Tg mice represent kainic acid (KA)-induced increase of TWIK-1 expression. ( A ) Representative GFP expression in a brain slice from saline or KA-treated TWIK-1 BAC-GFP Tg mice. Scale bar, 1000 μm. ( B ) Representative GFP expression in the DG from saline or KA-treated TWIK-1 BAC-GFP Tg mice. Scale bar, 200 μm. Enlarged inset from the experiment. Scale bar, 50 μm. ( C ) Quantification of relative mean GFP intensity of the granule cell layer. ( D ) Representative Kcnk1 mRNA fluorescence in situ hybridization (FISH) images of DG from saline- or KA-treated mice. Scale bar, 100 μm. ( E ) Quantification bar graph of Kcnk1 mRNA spot density in the granule cell layer from saline- or KA-treated mice. ( F ) Representative TWIK-1 immunofluorescence images of DG from saline- or KA-treated mice. Scale bar, 200 μm. ( G ) Quantification bar graph of the relative mean TWIK-1 immunofluorescence in the granule cell layer of saline- or KA-treated mice. Raw data are listed in Supplementary Materials Table S1 . **** p

    Article Snippet: The following antibodies were used: chicken anti-GFP (Abcam, Cat#; ab136970, 1:300); rabbit anti-TWIK-1 (Alomone labs, Cat#; APC-10, 1:200); mouse anti-NeuN (Abcam, Cat#; ab104224, 1:100); rabbit anti-GAD67 (GeneTex, Cat#; GTX113190, 1:300); guinea pig anti-doublecortin (Millipore, Cat#; AB2253, 1:100); rabbit anti-calbindin (Swant, Cat#; CB38, 1:2000); rat anti-Ki67 (Invitrogen, Cat#; 14-5698-85, 1:200); rat anti-GFAP (Invitrogen, Cat#; 13-0300, 1:500); rabbit anti-NG2 (Millipore, Cat#; AB5320, 1:300); and Alexa Fluor 488-, 594-, and 647-conjugated secondary antibodies (Jackson ImmunoResearch, 1:300).

    Techniques: BAC Assay, Mouse Assay, Expressing, Slice Preparation, Fluorescence, In Situ Hybridization, Fluorescence In Situ Hybridization, Immunofluorescence