Journal: Frontiers in Cellular Neuroscience
Article Title: Notoginsenoside R1–Induced Neuronal Repair in Models of Alzheimer Disease Is Associated With an Alteration in Neuronal Hyperexcitability, Which Is Regulated by Nav
Figure Lengend Snippet: Notoginsenoside R1 altered the distribution of Nav1.1α and cleavage of Navβ2 in the brains of APP/PS1 mice. Representative expression (A,E) and densitometric quantification of BACE1 (B) , Navβ2 full-length (C) , Navβ2-CTF (D) , total expression of Nav1.1α (F) extracellular Nav1.1α (G) , and intracellular Nav1.1α (H) protein in the cortex and hippocampus lysates from WT mice, APP/PS1 mice, or APP/PS1 + R1 mice, respectively. Data are shown as mean ± SD ( n = 15). * p
Article Snippet: Proteins were then transferred to nitrocellulose membranes and incubated with primary antibodies against BACE1 (1:1,000; cat. no. ab2077; Abcam), Nav1.1α (1:800; cat. no. ASC-001; Alomone), or Navβ2 (1:500; cat. no. ASC-007; Alomone).
Techniques: Mouse Assay, Expressing