Journal: CNS Neuroscience & Therapeutics
Article Title: Effects of Acetazolamide on Transient K+ Currents and Action Potentials in Nodose Ganglion Neurons of Adult Rats
Figure Lengend Snippet: Example of mRNA encoding Kv 1.4 and Kv 4 family channel proteins in adult rat nodose ganglia. Gel electrophoresis of PCR products obtained from nodose ganglia. a ), A nucleotide size ladder in 100 bp increments. PCR products obtained with the primer for Kv 1.4 (119 bp, b ), Kv 4.1 (467 bp, c ), Kv 4.2 (110 bp, d ), Kv 4.3 (400 bp, e ), and GAPDH (418 bp, f ). Negative control (no reverse transcription, g ).
Article Snippet: In order to demonstrate Kv 1.4 and CA-II, Kv 4.1 and CA-II, Kv 4.2 and CA-II, and Kv 4.3 and CA-II fluorescent double stainings, sections were separately exposed for a period of 2 days to mix the first antibody solution with rabbit anti-Kv 1.4 (1:160, Alomone Labs Ltd., Jerusalem, Israel) and sheep anti-CA-II polyclonal (1:500, Biogenesis, USA), rabbit anti-Kv 4.1 (1:160, Alomone Labs Ltd., Jerusalem) and sheep anti-CA-II polyclonal (1:500, Biogenesis), rabbit anti-Kv 4.2 (1:160, Chemicon) and sheep anti-CA-II polyclonal (1:500, Biogenesis) and rabbit anti-Kv 4.3 (1:160, Alomone Labs Ltd., Jerusalem) and sheep anti-CA-II polyclonal (1:500, Biogenesis) at 4°C followed by incubation in a secondary antibody solution with Alexa® 568 goat anti-rabbit IgG (1:1000, Molecular Probes, Kv 1.4, Kv 4.1–4.3, red, USA) and donkey anti IgG sheep-FITC (1:1000, CA-II, green, Chemicon) for 24 h at room temperature, respectively.
Techniques: Nucleic Acid Electrophoresis, Polymerase Chain Reaction, Negative Control