Real-time Polymerase Chain Reaction:Article Title: Melanoma exosomal hsa-miR-221-5p suppresses keratinocyte LL-37 to promote tumor progression.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER ReverTra Ace qPCR RT Master Mix Toyobo Cat. # FSQ-201 ReverTra Ace qPCR RT Master Mix with gDNA Remover Toyobo Cat. # FSQ-301 QuantiNova® SYBR Green PCR Kit Qiagen Cat. # 208156 High-Sensitivity RNA ScreenTape Agilent Technologies Cat. # 5067-5579 Polyfast Transfection Reagent MedChemExpress Cat. # HY-K1014 NucleoBond Xtra Maxi EF plasmid purification kit Macherey-Nagel, Düren, Germany N/A Deposited data RNA sequencing data This paper GEO: GSE334049 TCGA transcriptome data UCSC Xena https://xenabrowser.net/datapages/ GTEx transcriptome data UCSC Xena https://xenabrowser.net/datapages/ MicroRNA sequencing data Xiao et al.31 GEO: GSE35387 Experimental models: Cell lines A375 human melanoma cells ATCC Cat. # CRL-1619 Normal human epidermal keratinocytes Kurabo Cat. # KK-4009 Normal human epidermal melanocytes, adult (NHEM[AD]) Kurabo Cat. # KM-4109 HaCaT immortalized human keratinocytes Cell Lines Service Cat. # 300493-ACADEMIC Human colorectal adenocarcinoma cells (HT-29-Luc cells) JCRB Cell Bank Cat. # JCRB1383 Human chronic myelogenous leukemia cells (K562-Luc cells) JCRB Cell Bank Cat. # JCRB1402 B16 mouse melanoma cell line Sigma‒Aldrich Cat. # SCC420 B16-F0-Luc cells JCRB Cell Bank Cat. # JCRB1474 HEK-293 T human embryonic kidney cells RIKEN BRC CELL BANK Cat. # RCB2202 Experimental models: Organisms/strains Mouse: C57BL/6 mice Japan SLC N/A Mouse: Tg[Krt14-cre]1Amc/J The Jackson Laboratory N/A Mouse: Campf/f mice Dr. Richard L. Gallo (University of California, San Diego) N/A Oligonucleotides Primers for genotyping of K14Cre and Camp This study See Table S1 Primers for human and mouse qRT‒PCR This study See Table S2 hsa-miR-17-5p mimic MedChemExpress Cat. # R00326 hsa-miR-221-5p mimic MedChemExpress Cat. # R00465 hsa-miR-17-5p inhibitor MedChemExpress Cat. # RI00326 hsa-miR-221-5p inhibitor MedChemExpress Cat. # RI00465 MicroRNA mimic negative control MedChemExpress Cat. # HY-R04602 MicroRNA inhibitor negative control MedChemExpress Cat. # HY-RI04602 Recombinant DNA Plasmid: pLP1, pLP2, pLP/VSVG Invitrogen ViraPower Packaging Mix, Cat. # K4950-00 Plasmid: shRNA-109 This study N/A Plasmid: shRNA-360 This study N/A Plasmid: shRNA-459 This study N/A Plasmid: pCAMP-6×His-Bsd This study N/A Plasmid: pCAMP-HA-Bsd This study N/A Plasmid: pCAMP-6×His-Hygro This study N/A pMRX-IP-HaloTag7-LC3 RIKEN BRC RDB19968 (Continued on next page) 18 Cell Reports 45, 117837, August 25, 2026
SYBR Green Assay:Article Title: Melanoma exosomal hsa-miR-221-5p suppresses keratinocyte LL-37 to promote tumor progression.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER ReverTra Ace qPCR RT Master Mix Toyobo Cat. # FSQ-201 ReverTra Ace qPCR RT Master Mix with gDNA Remover Toyobo Cat. # FSQ-301 QuantiNova® SYBR Green PCR Kit Qiagen Cat. # 208156 High-Sensitivity RNA ScreenTape Agilent Technologies Cat. # 5067-5579 Polyfast Transfection Reagent MedChemExpress Cat. # HY-K1014 NucleoBond Xtra Maxi EF plasmid purification kit Macherey-Nagel, Düren, Germany N/A Deposited data RNA sequencing data This paper GEO: GSE334049 TCGA transcriptome data UCSC Xena https://xenabrowser.net/datapages/ GTEx transcriptome data UCSC Xena https://xenabrowser.net/datapages/ MicroRNA sequencing data Xiao et al.31 GEO: GSE35387 Experimental models: Cell lines A375 human melanoma cells ATCC Cat. # CRL-1619 Normal human epidermal keratinocytes Kurabo Cat. # KK-4009 Normal human epidermal melanocytes, adult (NHEM[AD]) Kurabo Cat. # KM-4109 HaCaT immortalized human keratinocytes Cell Lines Service Cat. # 300493-ACADEMIC Human colorectal adenocarcinoma cells (HT-29-Luc cells) JCRB Cell Bank Cat. # JCRB1383 Human chronic myelogenous leukemia cells (K562-Luc cells) JCRB Cell Bank Cat. # JCRB1402 B16 mouse melanoma cell line Sigma‒Aldrich Cat. # SCC420 B16-F0-Luc cells JCRB Cell Bank Cat. # JCRB1474 HEK-293 T human embryonic kidney cells RIKEN BRC CELL BANK Cat. # RCB2202 Experimental models: Organisms/strains Mouse: C57BL/6 mice Japan SLC N/A Mouse: Tg[Krt14-cre]1Amc/J The Jackson Laboratory N/A Mouse: Campf/f mice Dr. Richard L. Gallo (University of California, San Diego) N/A Oligonucleotides Primers for genotyping of K14Cre and Camp This study See Table S1 Primers for human and mouse qRT‒PCR This study See Table S2 hsa-miR-17-5p mimic MedChemExpress Cat. # R00326 hsa-miR-221-5p mimic MedChemExpress Cat. # R00465 hsa-miR-17-5p inhibitor MedChemExpress Cat. # RI00326 hsa-miR-221-5p inhibitor MedChemExpress Cat. # RI00465 MicroRNA mimic negative control MedChemExpress Cat. # HY-R04602 MicroRNA inhibitor negative control MedChemExpress Cat. # HY-RI04602 Recombinant DNA Plasmid: pLP1, pLP2, pLP/VSVG Invitrogen ViraPower Packaging Mix, Cat. # K4950-00 Plasmid: shRNA-109 This study N/A Plasmid: shRNA-360 This study N/A Plasmid: shRNA-459 This study N/A Plasmid: pCAMP-6×His-Bsd This study N/A Plasmid: pCAMP-HA-Bsd This study N/A Plasmid: pCAMP-6×His-Hygro This study N/A pMRX-IP-HaloTag7-LC3 RIKEN BRC RDB19968 (Continued on next page) 18 Cell Reports 45, 117837, August 25, 2026
Polymerase Chain Reaction:Article Title: Melanoma exosomal hsa-miR-221-5p suppresses keratinocyte LL-37 to promote tumor progression.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER ReverTra Ace qPCR RT Master Mix Toyobo Cat. # FSQ-201 ReverTra Ace qPCR RT Master Mix with gDNA Remover Toyobo Cat. # FSQ-301 QuantiNova® SYBR Green PCR Kit Qiagen Cat. # 208156 High-Sensitivity RNA ScreenTape Agilent Technologies Cat. # 5067-5579 Polyfast Transfection Reagent MedChemExpress Cat. # HY-K1014 NucleoBond Xtra Maxi EF plasmid purification kit Macherey-Nagel, Düren, Germany N/A Deposited data RNA sequencing data This paper GEO: GSE334049 TCGA transcriptome data UCSC Xena https://xenabrowser.net/datapages/ GTEx transcriptome data UCSC Xena https://xenabrowser.net/datapages/ MicroRNA sequencing data Xiao et al.31 GEO: GSE35387 Experimental models: Cell lines A375 human melanoma cells ATCC Cat. # CRL-1619 Normal human epidermal keratinocytes Kurabo Cat. # KK-4009 Normal human epidermal melanocytes, adult (NHEM[AD]) Kurabo Cat. # KM-4109 HaCaT immortalized human keratinocytes Cell Lines Service Cat. # 300493-ACADEMIC Human colorectal adenocarcinoma cells (HT-29-Luc cells) JCRB Cell Bank Cat. # JCRB1383 Human chronic myelogenous leukemia cells (K562-Luc cells) JCRB Cell Bank Cat. # JCRB1402 B16 mouse melanoma cell line Sigma‒Aldrich Cat. # SCC420 B16-F0-Luc cells JCRB Cell Bank Cat. # JCRB1474 HEK-293 T human embryonic kidney cells RIKEN BRC CELL BANK Cat. # RCB2202 Experimental models: Organisms/strains Mouse: C57BL/6 mice Japan SLC N/A Mouse: Tg[Krt14-cre]1Amc/J The Jackson Laboratory N/A Mouse: Campf/f mice Dr. Richard L. Gallo (University of California, San Diego) N/A Oligonucleotides Primers for genotyping of K14Cre and Camp This study See Table S1 Primers for human and mouse qRT‒PCR This study See Table S2 hsa-miR-17-5p mimic MedChemExpress Cat. # R00326 hsa-miR-221-5p mimic MedChemExpress Cat. # R00465 hsa-miR-17-5p inhibitor MedChemExpress Cat. # RI00326 hsa-miR-221-5p inhibitor MedChemExpress Cat. # RI00465 MicroRNA mimic negative control MedChemExpress Cat. # HY-R04602 MicroRNA inhibitor negative control MedChemExpress Cat. # HY-RI04602 Recombinant DNA Plasmid: pLP1, pLP2, pLP/VSVG Invitrogen ViraPower Packaging Mix, Cat. # K4950-00 Plasmid: shRNA-109 This study N/A Plasmid: shRNA-360 This study N/A Plasmid: shRNA-459 This study N/A Plasmid: pCAMP-6×His-Bsd This study N/A Plasmid: pCAMP-HA-Bsd This study N/A Plasmid: pCAMP-6×His-Hygro This study N/A pMRX-IP-HaloTag7-LC3 RIKEN BRC RDB19968 (Continued on next page) 18 Cell Reports 45, 117837, August 25, 2026
Transfection:Article Title: Melanoma exosomal hsa-miR-221-5p suppresses keratinocyte LL-37 to promote tumor progression.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER ReverTra Ace qPCR RT Master Mix Toyobo Cat. # FSQ-201 ReverTra Ace qPCR RT Master Mix with gDNA Remover Toyobo Cat. # FSQ-301 QuantiNova® SYBR Green PCR Kit Qiagen Cat. # 208156 High-Sensitivity RNA ScreenTape Agilent Technologies Cat. # 5067-5579 Polyfast Transfection Reagent MedChemExpress Cat. # HY-K1014 NucleoBond Xtra Maxi EF plasmid purification kit Macherey-Nagel, Düren, Germany N/A Deposited data RNA sequencing data This paper GEO: GSE334049 TCGA transcriptome data UCSC Xena https://xenabrowser.net/datapages/ GTEx transcriptome data UCSC Xena https://xenabrowser.net/datapages/ MicroRNA sequencing data Xiao et al.31 GEO: GSE35387 Experimental models: Cell lines A375 human melanoma cells ATCC Cat. # CRL-1619 Normal human epidermal keratinocytes Kurabo Cat. # KK-4009 Normal human epidermal melanocytes, adult (NHEM[AD]) Kurabo Cat. # KM-4109 HaCaT immortalized human keratinocytes Cell Lines Service Cat. # 300493-ACADEMIC Human colorectal adenocarcinoma cells (HT-29-Luc cells) JCRB Cell Bank Cat. # JCRB1383 Human chronic myelogenous leukemia cells (K562-Luc cells) JCRB Cell Bank Cat. # JCRB1402 B16 mouse melanoma cell line Sigma‒Aldrich Cat. # SCC420 B16-F0-Luc cells JCRB Cell Bank Cat. # JCRB1474 HEK-293 T human embryonic kidney cells RIKEN BRC CELL BANK Cat. # RCB2202 Experimental models: Organisms/strains Mouse: C57BL/6 mice Japan SLC N/A Mouse: Tg[Krt14-cre]1Amc/J The Jackson Laboratory N/A Mouse: Campf/f mice Dr. Richard L. Gallo (University of California, San Diego) N/A Oligonucleotides Primers for genotyping of K14Cre and Camp This study See Table S1 Primers for human and mouse qRT‒PCR This study See Table S2 hsa-miR-17-5p mimic MedChemExpress Cat. # R00326 hsa-miR-221-5p mimic MedChemExpress Cat. # R00465 hsa-miR-17-5p inhibitor MedChemExpress Cat. # RI00326 hsa-miR-221-5p inhibitor MedChemExpress Cat. # RI00465 MicroRNA mimic negative control MedChemExpress Cat. # HY-R04602 MicroRNA inhibitor negative control MedChemExpress Cat. # HY-RI04602 Recombinant DNA Plasmid: pLP1, pLP2, pLP/VSVG Invitrogen ViraPower Packaging Mix, Cat. # K4950-00 Plasmid: shRNA-109 This study N/A Plasmid: shRNA-360 This study N/A Plasmid: shRNA-459 This study N/A Plasmid: pCAMP-6×His-Bsd This study N/A Plasmid: pCAMP-HA-Bsd This study N/A Plasmid: pCAMP-6×His-Hygro This study N/A pMRX-IP-HaloTag7-LC3 RIKEN BRC RDB19968 (Continued on next page) 18 Cell Reports 45, 117837, August 25, 2026
Plasmid Preparation:Article Title: Melanoma exosomal hsa-miR-221-5p suppresses keratinocyte LL-37 to promote tumor progression.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER ReverTra Ace qPCR RT Master Mix Toyobo Cat. # FSQ-201 ReverTra Ace qPCR RT Master Mix with gDNA Remover Toyobo Cat. # FSQ-301 QuantiNova® SYBR Green PCR Kit Qiagen Cat. # 208156 High-Sensitivity RNA ScreenTape Agilent Technologies Cat. # 5067-5579 Polyfast Transfection Reagent MedChemExpress Cat. # HY-K1014 NucleoBond Xtra Maxi EF plasmid purification kit Macherey-Nagel, Düren, Germany N/A Deposited data RNA sequencing data This paper GEO: GSE334049 TCGA transcriptome data UCSC Xena https://xenabrowser.net/datapages/ GTEx transcriptome data UCSC Xena https://xenabrowser.net/datapages/ MicroRNA sequencing data Xiao et al.31 GEO: GSE35387 Experimental models: Cell lines A375 human melanoma cells ATCC Cat. # CRL-1619 Normal human epidermal keratinocytes Kurabo Cat. # KK-4009 Normal human epidermal melanocytes, adult (NHEM[AD]) Kurabo Cat. # KM-4109 HaCaT immortalized human keratinocytes Cell Lines Service Cat. # 300493-ACADEMIC Human colorectal adenocarcinoma cells (HT-29-Luc cells) JCRB Cell Bank Cat. # JCRB1383 Human chronic myelogenous leukemia cells (K562-Luc cells) JCRB Cell Bank Cat. # JCRB1402 B16 mouse melanoma cell line Sigma‒Aldrich Cat. # SCC420 B16-F0-Luc cells JCRB Cell Bank Cat. # JCRB1474 HEK-293 T human embryonic kidney cells RIKEN BRC CELL BANK Cat. # RCB2202 Experimental models: Organisms/strains Mouse: C57BL/6 mice Japan SLC N/A Mouse: Tg[Krt14-cre]1Amc/J The Jackson Laboratory N/A Mouse: Campf/f mice Dr. Richard L. Gallo (University of California, San Diego) N/A Oligonucleotides Primers for genotyping of K14Cre and Camp This study See Table S1 Primers for human and mouse qRT‒PCR This study See Table S2 hsa-miR-17-5p mimic MedChemExpress Cat. # R00326 hsa-miR-221-5p mimic MedChemExpress Cat. # R00465 hsa-miR-17-5p inhibitor MedChemExpress Cat. # RI00326 hsa-miR-221-5p inhibitor MedChemExpress Cat. # RI00465 MicroRNA mimic negative control MedChemExpress Cat. # HY-R04602 MicroRNA inhibitor negative control MedChemExpress Cat. # HY-RI04602 Recombinant DNA Plasmid: pLP1, pLP2, pLP/VSVG Invitrogen ViraPower Packaging Mix, Cat. # K4950-00 Plasmid: shRNA-109 This study N/A Plasmid: shRNA-360 This study N/A Plasmid: shRNA-459 This study N/A Plasmid: pCAMP-6×His-Bsd This study N/A Plasmid: pCAMP-HA-Bsd This study N/A Plasmid: pCAMP-6×His-Hygro This study N/A pMRX-IP-HaloTag7-LC3 RIKEN BRC RDB19968 (Continued on next page) 18 Cell Reports 45, 117837, August 25, 2026
Purification:Article Title: Melanoma exosomal hsa-miR-221-5p suppresses keratinocyte LL-37 to promote tumor progression.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER ReverTra Ace qPCR RT Master Mix Toyobo Cat. # FSQ-201 ReverTra Ace qPCR RT Master Mix with gDNA Remover Toyobo Cat. # FSQ-301 QuantiNova® SYBR Green PCR Kit Qiagen Cat. # 208156 High-Sensitivity RNA ScreenTape Agilent Technologies Cat. # 5067-5579 Polyfast Transfection Reagent MedChemExpress Cat. # HY-K1014 NucleoBond Xtra Maxi EF plasmid purification kit Macherey-Nagel, Düren, Germany N/A Deposited data RNA sequencing data This paper GEO: GSE334049 TCGA transcriptome data UCSC Xena https://xenabrowser.net/datapages/ GTEx transcriptome data UCSC Xena https://xenabrowser.net/datapages/ MicroRNA sequencing data Xiao et al.31 GEO: GSE35387 Experimental models: Cell lines A375 human melanoma cells ATCC Cat. # CRL-1619 Normal human epidermal keratinocytes Kurabo Cat. # KK-4009 Normal human epidermal melanocytes, adult (NHEM[AD]) Kurabo Cat. # KM-4109 HaCaT immortalized human keratinocytes Cell Lines Service Cat. # 300493-ACADEMIC Human colorectal adenocarcinoma cells (HT-29-Luc cells) JCRB Cell Bank Cat. # JCRB1383 Human chronic myelogenous leukemia cells (K562-Luc cells) JCRB Cell Bank Cat. # JCRB1402 B16 mouse melanoma cell line Sigma‒Aldrich Cat. # SCC420 B16-F0-Luc cells JCRB Cell Bank Cat. # JCRB1474 HEK-293 T human embryonic kidney cells RIKEN BRC CELL BANK Cat. # RCB2202 Experimental models: Organisms/strains Mouse: C57BL/6 mice Japan SLC N/A Mouse: Tg[Krt14-cre]1Amc/J The Jackson Laboratory N/A Mouse: Campf/f mice Dr. Richard L. Gallo (University of California, San Diego) N/A Oligonucleotides Primers for genotyping of K14Cre and Camp This study See Table S1 Primers for human and mouse qRT‒PCR This study See Table S2 hsa-miR-17-5p mimic MedChemExpress Cat. # R00326 hsa-miR-221-5p mimic MedChemExpress Cat. # R00465 hsa-miR-17-5p inhibitor MedChemExpress Cat. # RI00326 hsa-miR-221-5p inhibitor MedChemExpress Cat. # RI00465 MicroRNA mimic negative control MedChemExpress Cat. # HY-R04602 MicroRNA inhibitor negative control MedChemExpress Cat. # HY-RI04602 Recombinant DNA Plasmid: pLP1, pLP2, pLP/VSVG Invitrogen ViraPower Packaging Mix, Cat. # K4950-00 Plasmid: shRNA-109 This study N/A Plasmid: shRNA-360 This study N/A Plasmid: shRNA-459 This study N/A Plasmid: pCAMP-6×His-Bsd This study N/A Plasmid: pCAMP-HA-Bsd This study N/A Plasmid: pCAMP-6×His-Hygro This study N/A pMRX-IP-HaloTag7-LC3 RIKEN BRC RDB19968 (Continued on next page) 18 Cell Reports 45, 117837, August 25, 2026
RNA Sequencing:Article Title: Melanoma exosomal hsa-miR-221-5p suppresses keratinocyte LL-37 to promote tumor progression.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER ReverTra Ace qPCR RT Master Mix Toyobo Cat. # FSQ-201 ReverTra Ace qPCR RT Master Mix with gDNA Remover Toyobo Cat. # FSQ-301 QuantiNova® SYBR Green PCR Kit Qiagen Cat. # 208156 High-Sensitivity RNA ScreenTape Agilent Technologies Cat. # 5067-5579 Polyfast Transfection Reagent MedChemExpress Cat. # HY-K1014 NucleoBond Xtra Maxi EF plasmid purification kit Macherey-Nagel, Düren, Germany N/A Deposited data RNA sequencing data This paper GEO: GSE334049 TCGA transcriptome data UCSC Xena https://xenabrowser.net/datapages/ GTEx transcriptome data UCSC Xena https://xenabrowser.net/datapages/ MicroRNA sequencing data Xiao et al.31 GEO: GSE35387 Experimental models: Cell lines A375 human melanoma cells ATCC Cat. # CRL-1619 Normal human epidermal keratinocytes Kurabo Cat. # KK-4009 Normal human epidermal melanocytes, adult (NHEM[AD]) Kurabo Cat. # KM-4109 HaCaT immortalized human keratinocytes Cell Lines Service Cat. # 300493-ACADEMIC Human colorectal adenocarcinoma cells (HT-29-Luc cells) JCRB Cell Bank Cat. # JCRB1383 Human chronic myelogenous leukemia cells (K562-Luc cells) JCRB Cell Bank Cat. # JCRB1402 B16 mouse melanoma cell line Sigma‒Aldrich Cat. # SCC420 B16-F0-Luc cells JCRB Cell Bank Cat. # JCRB1474 HEK-293 T human embryonic kidney cells RIKEN BRC CELL BANK Cat. # RCB2202 Experimental models: Organisms/strains Mouse: C57BL/6 mice Japan SLC N/A Mouse: Tg[Krt14-cre]1Amc/J The Jackson Laboratory N/A Mouse: Campf/f mice Dr. Richard L. Gallo (University of California, San Diego) N/A Oligonucleotides Primers for genotyping of K14Cre and Camp This study See Table S1 Primers for human and mouse qRT‒PCR This study See Table S2 hsa-miR-17-5p mimic MedChemExpress Cat. # R00326 hsa-miR-221-5p mimic MedChemExpress Cat. # R00465 hsa-miR-17-5p inhibitor MedChemExpress Cat. # RI00326 hsa-miR-221-5p inhibitor MedChemExpress Cat. # RI00465 MicroRNA mimic negative control MedChemExpress Cat. # HY-R04602 MicroRNA inhibitor negative control MedChemExpress Cat. # HY-RI04602 Recombinant DNA Plasmid: pLP1, pLP2, pLP/VSVG Invitrogen ViraPower Packaging Mix, Cat. # K4950-00 Plasmid: shRNA-109 This study N/A Plasmid: shRNA-360 This study N/A Plasmid: shRNA-459 This study N/A Plasmid: pCAMP-6×His-Bsd This study N/A Plasmid: pCAMP-HA-Bsd This study N/A Plasmid: pCAMP-6×His-Hygro This study N/A pMRX-IP-HaloTag7-LC3 RIKEN BRC RDB19968 (Continued on next page) 18 Cell Reports 45, 117837, August 25, 2026
Sequencing:Article Title: Melanoma exosomal hsa-miR-221-5p suppresses keratinocyte LL-37 to promote tumor progression.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER ReverTra Ace qPCR RT Master Mix Toyobo Cat. # FSQ-201 ReverTra Ace qPCR RT Master Mix with gDNA Remover Toyobo Cat. # FSQ-301 QuantiNova® SYBR Green PCR Kit Qiagen Cat. # 208156 High-Sensitivity RNA ScreenTape Agilent Technologies Cat. # 5067-5579 Polyfast Transfection Reagent MedChemExpress Cat. # HY-K1014 NucleoBond Xtra Maxi EF plasmid purification kit Macherey-Nagel, Düren, Germany N/A Deposited data RNA sequencing data This paper GEO: GSE334049 TCGA transcriptome data UCSC Xena https://xenabrowser.net/datapages/ GTEx transcriptome data UCSC Xena https://xenabrowser.net/datapages/ MicroRNA sequencing data Xiao et al.31 GEO: GSE35387 Experimental models: Cell lines A375 human melanoma cells ATCC Cat. # CRL-1619 Normal human epidermal keratinocytes Kurabo Cat. # KK-4009 Normal human epidermal melanocytes, adult (NHEM[AD]) Kurabo Cat. # KM-4109 HaCaT immortalized human keratinocytes Cell Lines Service Cat. # 300493-ACADEMIC Human colorectal adenocarcinoma cells (HT-29-Luc cells) JCRB Cell Bank Cat. # JCRB1383 Human chronic myelogenous leukemia cells (K562-Luc cells) JCRB Cell Bank Cat. # JCRB1402 B16 mouse melanoma cell line Sigma‒Aldrich Cat. # SCC420 B16-F0-Luc cells JCRB Cell Bank Cat. # JCRB1474 HEK-293 T human embryonic kidney cells RIKEN BRC CELL BANK Cat. # RCB2202 Experimental models: Organisms/strains Mouse: C57BL/6 mice Japan SLC N/A Mouse: Tg[Krt14-cre]1Amc/J The Jackson Laboratory N/A Mouse: Campf/f mice Dr. Richard L. Gallo (University of California, San Diego) N/A Oligonucleotides Primers for genotyping of K14Cre and Camp This study See Table S1 Primers for human and mouse qRT‒PCR This study See Table S2 hsa-miR-17-5p mimic MedChemExpress Cat. # R00326 hsa-miR-221-5p mimic MedChemExpress Cat. # R00465 hsa-miR-17-5p inhibitor MedChemExpress Cat. # RI00326 hsa-miR-221-5p inhibitor MedChemExpress Cat. # RI00465 MicroRNA mimic negative control MedChemExpress Cat. # HY-R04602 MicroRNA inhibitor negative control MedChemExpress Cat. # HY-RI04602 Recombinant DNA Plasmid: pLP1, pLP2, pLP/VSVG Invitrogen ViraPower Packaging Mix, Cat. # K4950-00 Plasmid: shRNA-109 This study N/A Plasmid: shRNA-360 This study N/A Plasmid: shRNA-459 This study N/A Plasmid: pCAMP-6×His-Bsd This study N/A Plasmid: pCAMP-HA-Bsd This study N/A Plasmid: pCAMP-6×His-Hygro This study N/A pMRX-IP-HaloTag7-LC3 RIKEN BRC RDB19968 (Continued on next page) 18 Cell Reports 45, 117837, August 25, 2026
Negative Control:Article Title: Melanoma exosomal hsa-miR-221-5p suppresses keratinocyte LL-37 to promote tumor progression.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER ReverTra Ace qPCR RT Master Mix Toyobo Cat. # FSQ-201 ReverTra Ace qPCR RT Master Mix with gDNA Remover Toyobo Cat. # FSQ-301 QuantiNova® SYBR Green PCR Kit Qiagen Cat. # 208156 High-Sensitivity RNA ScreenTape Agilent Technologies Cat. # 5067-5579 Polyfast Transfection Reagent MedChemExpress Cat. # HY-K1014 NucleoBond Xtra Maxi EF plasmid purification kit Macherey-Nagel, Düren, Germany N/A Deposited data RNA sequencing data This paper GEO: GSE334049 TCGA transcriptome data UCSC Xena https://xenabrowser.net/datapages/ GTEx transcriptome data UCSC Xena https://xenabrowser.net/datapages/ MicroRNA sequencing data Xiao et al.31 GEO: GSE35387 Experimental models: Cell lines A375 human melanoma cells ATCC Cat. # CRL-1619 Normal human epidermal keratinocytes Kurabo Cat. # KK-4009 Normal human epidermal melanocytes, adult (NHEM[AD]) Kurabo Cat. # KM-4109 HaCaT immortalized human keratinocytes Cell Lines Service Cat. # 300493-ACADEMIC Human colorectal adenocarcinoma cells (HT-29-Luc cells) JCRB Cell Bank Cat. # JCRB1383 Human chronic myelogenous leukemia cells (K562-Luc cells) JCRB Cell Bank Cat. # JCRB1402 B16 mouse melanoma cell line Sigma‒Aldrich Cat. # SCC420 B16-F0-Luc cells JCRB Cell Bank Cat. # JCRB1474 HEK-293 T human embryonic kidney cells RIKEN BRC CELL BANK Cat. # RCB2202 Experimental models: Organisms/strains Mouse: C57BL/6 mice Japan SLC N/A Mouse: Tg[Krt14-cre]1Amc/J The Jackson Laboratory N/A Mouse: Campf/f mice Dr. Richard L. Gallo (University of California, San Diego) N/A Oligonucleotides Primers for genotyping of K14Cre and Camp This study See Table S1 Primers for human and mouse qRT‒PCR This study See Table S2 hsa-miR-17-5p mimic MedChemExpress Cat. # R00326 hsa-miR-221-5p mimic MedChemExpress Cat. # R00465 hsa-miR-17-5p inhibitor MedChemExpress Cat. # RI00326 hsa-miR-221-5p inhibitor MedChemExpress Cat. # RI00465 MicroRNA mimic negative control MedChemExpress Cat. # HY-R04602 MicroRNA inhibitor negative control MedChemExpress Cat. # HY-RI04602 Recombinant DNA Plasmid: pLP1, pLP2, pLP/VSVG Invitrogen ViraPower Packaging Mix, Cat. # K4950-00 Plasmid: shRNA-109 This study N/A Plasmid: shRNA-360 This study N/A Plasmid: shRNA-459 This study N/A Plasmid: pCAMP-6×His-Bsd This study N/A Plasmid: pCAMP-HA-Bsd This study N/A Plasmid: pCAMP-6×His-Hygro This study N/A pMRX-IP-HaloTag7-LC3 RIKEN BRC RDB19968 (Continued on next page) 18 Cell Reports 45, 117837, August 25, 2026
Recombinant:Article Title: Melanoma exosomal hsa-miR-221-5p suppresses keratinocyte LL-37 to promote tumor progression.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER ReverTra Ace qPCR RT Master Mix Toyobo Cat. # FSQ-201 ReverTra Ace qPCR RT Master Mix with gDNA Remover Toyobo Cat. # FSQ-301 QuantiNova® SYBR Green PCR Kit Qiagen Cat. # 208156 High-Sensitivity RNA ScreenTape Agilent Technologies Cat. # 5067-5579 Polyfast Transfection Reagent MedChemExpress Cat. # HY-K1014 NucleoBond Xtra Maxi EF plasmid purification kit Macherey-Nagel, Düren, Germany N/A Deposited data RNA sequencing data This paper GEO: GSE334049 TCGA transcriptome data UCSC Xena https://xenabrowser.net/datapages/ GTEx transcriptome data UCSC Xena https://xenabrowser.net/datapages/ MicroRNA sequencing data Xiao et al.31 GEO: GSE35387 Experimental models: Cell lines A375 human melanoma cells ATCC Cat. # CRL-1619 Normal human epidermal keratinocytes Kurabo Cat. # KK-4009 Normal human epidermal melanocytes, adult (NHEM[AD]) Kurabo Cat. # KM-4109 HaCaT immortalized human keratinocytes Cell Lines Service Cat. # 300493-ACADEMIC Human colorectal adenocarcinoma cells (HT-29-Luc cells) JCRB Cell Bank Cat. # JCRB1383 Human chronic myelogenous leukemia cells (K562-Luc cells) JCRB Cell Bank Cat. # JCRB1402 B16 mouse melanoma cell line Sigma‒Aldrich Cat. # SCC420 B16-F0-Luc cells JCRB Cell Bank Cat. # JCRB1474 HEK-293 T human embryonic kidney cells RIKEN BRC CELL BANK Cat. # RCB2202 Experimental models: Organisms/strains Mouse: C57BL/6 mice Japan SLC N/A Mouse: Tg[Krt14-cre]1Amc/J The Jackson Laboratory N/A Mouse: Campf/f mice Dr. Richard L. Gallo (University of California, San Diego) N/A Oligonucleotides Primers for genotyping of K14Cre and Camp This study See Table S1 Primers for human and mouse qRT‒PCR This study See Table S2 hsa-miR-17-5p mimic MedChemExpress Cat. # R00326 hsa-miR-221-5p mimic MedChemExpress Cat. # R00465 hsa-miR-17-5p inhibitor MedChemExpress Cat. # RI00326 hsa-miR-221-5p inhibitor MedChemExpress Cat. # RI00465 MicroRNA mimic negative control MedChemExpress Cat. # HY-R04602 MicroRNA inhibitor negative control MedChemExpress Cat. # HY-RI04602 Recombinant DNA Plasmid: pLP1, pLP2, pLP/VSVG Invitrogen ViraPower Packaging Mix, Cat. # K4950-00 Plasmid: shRNA-109 This study N/A Plasmid: shRNA-360 This study N/A Plasmid: shRNA-459 This study N/A Plasmid: pCAMP-6×His-Bsd This study N/A Plasmid: pCAMP-HA-Bsd This study N/A Plasmid: pCAMP-6×His-Hygro This study N/A pMRX-IP-HaloTag7-LC3 RIKEN BRC RDB19968 (Continued on next page) 18 Cell Reports 45, 117837, August 25, 2026
shRNA:Article Title: Melanoma exosomal hsa-miR-221-5p suppresses keratinocyte LL-37 to promote tumor progression.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER ReverTra Ace qPCR RT Master Mix Toyobo Cat. # FSQ-201 ReverTra Ace qPCR RT Master Mix with gDNA Remover Toyobo Cat. # FSQ-301 QuantiNova® SYBR Green PCR Kit Qiagen Cat. # 208156 High-Sensitivity RNA ScreenTape Agilent Technologies Cat. # 5067-5579 Polyfast Transfection Reagent MedChemExpress Cat. # HY-K1014 NucleoBond Xtra Maxi EF plasmid purification kit Macherey-Nagel, Düren, Germany N/A Deposited data RNA sequencing data This paper GEO: GSE334049 TCGA transcriptome data UCSC Xena https://xenabrowser.net/datapages/ GTEx transcriptome data UCSC Xena https://xenabrowser.net/datapages/ MicroRNA sequencing data Xiao et al.31 GEO: GSE35387 Experimental models: Cell lines A375 human melanoma cells ATCC Cat. # CRL-1619 Normal human epidermal keratinocytes Kurabo Cat. # KK-4009 Normal human epidermal melanocytes, adult (NHEM[AD]) Kurabo Cat. # KM-4109 HaCaT immortalized human keratinocytes Cell Lines Service Cat. # 300493-ACADEMIC Human colorectal adenocarcinoma cells (HT-29-Luc cells) JCRB Cell Bank Cat. # JCRB1383 Human chronic myelogenous leukemia cells (K562-Luc cells) JCRB Cell Bank Cat. # JCRB1402 B16 mouse melanoma cell line Sigma‒Aldrich Cat. # SCC420 B16-F0-Luc cells JCRB Cell Bank Cat. # JCRB1474 HEK-293 T human embryonic kidney cells RIKEN BRC CELL BANK Cat. # RCB2202 Experimental models: Organisms/strains Mouse: C57BL/6 mice Japan SLC N/A Mouse: Tg[Krt14-cre]1Amc/J The Jackson Laboratory N/A Mouse: Campf/f mice Dr. Richard L. Gallo (University of California, San Diego) N/A Oligonucleotides Primers for genotyping of K14Cre and Camp This study See Table S1 Primers for human and mouse qRT‒PCR This study See Table S2 hsa-miR-17-5p mimic MedChemExpress Cat. # R00326 hsa-miR-221-5p mimic MedChemExpress Cat. # R00465 hsa-miR-17-5p inhibitor MedChemExpress Cat. # RI00326 hsa-miR-221-5p inhibitor MedChemExpress Cat. # RI00465 MicroRNA mimic negative control MedChemExpress Cat. # HY-R04602 MicroRNA inhibitor negative control MedChemExpress Cat. # HY-RI04602 Recombinant DNA Plasmid: pLP1, pLP2, pLP/VSVG Invitrogen ViraPower Packaging Mix, Cat. # K4950-00 Plasmid: shRNA-109 This study N/A Plasmid: shRNA-360 This study N/A Plasmid: shRNA-459 This study N/A Plasmid: pCAMP-6×His-Bsd This study N/A Plasmid: pCAMP-HA-Bsd This study N/A Plasmid: pCAMP-6×His-Hygro This study N/A pMRX-IP-HaloTag7-LC3 RIKEN BRC RDB19968 (Continued on next page) 18 Cell Reports 45, 117837, August 25, 2026
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