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mir  (MedChemExpress)


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    MedChemExpress mir
    <t>miR-21-5p</t> <t>is</t> <t>the</t> key mediator of sEVs Hypo-5% in recovering cellular function of HG-HUVECs and HG-HDFs. (A) Representative EdU images showing the proliferation of HG-HUVECs treated with NC mimic, sEVs Hypo-5% + inhibitor NC , sEVs Hypo-5% + inhibitor miR-21-5p , or miR-21-5p mimic. Scale bar = 100 μm. (B) Quantification of (A) ( n = 3 per group). (C) Scratch wound-healing assay of HG-HUVECs in different groups. Scale bar = 100 μm. (D) Quantification analysis of (C) ( n = 4 per group). (E) Matrigel tube-formation assay of HG-HUVECs after different treatments. Scale bar = 100 μm. (F) Quantification analysis of segments in (E) ( n = 3 per group). (G) Representative EdU images showing the proliferation of HG-HDFs treated with NC mimic, sEVs Hypo-5% + inhibitor NC , sEVs Hypo-5% + inhibitor miR-21-5p , or miR-21-5p mimic. Scale bar = 100 μm. (H) Quantification analysis of (G) ( n = 3 per group). (I) Scratch wound-healing assay of HG-HDFs. Scale bar = 100 μm. (J) Quantification analysis of (I) ( n = 3 per group). (K) Transwell migration assay of HG-HDFs after different treatments. Scale bar = 100 μm. (L) Quantification analysis of (K) ( n = 4 per group). ns, not significant; * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001.
    Mir, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/HY-R00449/hsa-miR-21-5p+mimic/pmc13150076-295-29-34
    Average 94 stars, based on 1 article reviews
    mir - by Bioz Stars, 2026-11
    94/100 stars

    Images

    1) Product Images from "Hypoxia-Challenged sEVs-Engineered Nanofiber Scaffolds Accelerate Diabetic Wound Healing via Reversing Cellular Dysfunction of Skin Repair Cells"

    Article Title: Hypoxia-Challenged sEVs-Engineered Nanofiber Scaffolds Accelerate Diabetic Wound Healing via Reversing Cellular Dysfunction of Skin Repair Cells

    Journal: Research

    doi: 10.34133/research.1248

    miR-21-5p is the key mediator of sEVs Hypo-5% in recovering cellular function of HG-HUVECs and HG-HDFs. (A) Representative EdU images showing the proliferation of HG-HUVECs treated with NC mimic, sEVs Hypo-5% + inhibitor NC , sEVs Hypo-5% + inhibitor miR-21-5p , or miR-21-5p mimic. Scale bar = 100 μm. (B) Quantification of (A) ( n = 3 per group). (C) Scratch wound-healing assay of HG-HUVECs in different groups. Scale bar = 100 μm. (D) Quantification analysis of (C) ( n = 4 per group). (E) Matrigel tube-formation assay of HG-HUVECs after different treatments. Scale bar = 100 μm. (F) Quantification analysis of segments in (E) ( n = 3 per group). (G) Representative EdU images showing the proliferation of HG-HDFs treated with NC mimic, sEVs Hypo-5% + inhibitor NC , sEVs Hypo-5% + inhibitor miR-21-5p , or miR-21-5p mimic. Scale bar = 100 μm. (H) Quantification analysis of (G) ( n = 3 per group). (I) Scratch wound-healing assay of HG-HDFs. Scale bar = 100 μm. (J) Quantification analysis of (I) ( n = 3 per group). (K) Transwell migration assay of HG-HDFs after different treatments. Scale bar = 100 μm. (L) Quantification analysis of (K) ( n = 4 per group). ns, not significant; * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001.
    Figure Legend Snippet: miR-21-5p is the key mediator of sEVs Hypo-5% in recovering cellular function of HG-HUVECs and HG-HDFs. (A) Representative EdU images showing the proliferation of HG-HUVECs treated with NC mimic, sEVs Hypo-5% + inhibitor NC , sEVs Hypo-5% + inhibitor miR-21-5p , or miR-21-5p mimic. Scale bar = 100 μm. (B) Quantification of (A) ( n = 3 per group). (C) Scratch wound-healing assay of HG-HUVECs in different groups. Scale bar = 100 μm. (D) Quantification analysis of (C) ( n = 4 per group). (E) Matrigel tube-formation assay of HG-HUVECs after different treatments. Scale bar = 100 μm. (F) Quantification analysis of segments in (E) ( n = 3 per group). (G) Representative EdU images showing the proliferation of HG-HDFs treated with NC mimic, sEVs Hypo-5% + inhibitor NC , sEVs Hypo-5% + inhibitor miR-21-5p , or miR-21-5p mimic. Scale bar = 100 μm. (H) Quantification analysis of (G) ( n = 3 per group). (I) Scratch wound-healing assay of HG-HDFs. Scale bar = 100 μm. (J) Quantification analysis of (I) ( n = 3 per group). (K) Transwell migration assay of HG-HDFs after different treatments. Scale bar = 100 μm. (L) Quantification analysis of (K) ( n = 4 per group). ns, not significant; * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001.

    Techniques Used: Cell Function Assay, Wound Healing Assay, Tube Formation Assay, Transwell Migration Assay

    Related Articles

    Cell Function Assay:

    Article Title: Hypoxia-Challenged sEVs-Engineered Nanofiber Scaffolds Accelerate Diabetic Wound Healing via Reversing Cellular Dysfunction of Skin Repair Cells
    Article Snippet: Quantitative PCR was carried out using an ABIPRISM 7300 Sequence Detection System (Applied Biosystems, USA).Quantitative PCR was carried out using an ABIPRISM 7300 Sequence Detection System (Applied Biosystems, USA).. HG-HUVECs and HG-HDFs were treated with NC mimic (HY- R04602 , MCE, USA), sEVs Hypo-5% + inhibitor NC (HY-RI04602, MCE, USA), sEVs Hypo-5% + inhibitor miR-21-5p (HY-RI00449, MCE), or miR-21-5p mimic (HY- R00449 , MCE).. Total RNA was extracted using the same RNA extraction kit (YI SHAN Biotechnology, China).Total RNA was extracted using the same RNA extraction kit (YI SHAN Biotechnology, China).

    Article Title: Hypoxia-Challenged sEVs-Engineered Nanofiber Scaffolds Accelerate Diabetic Wound Healing via Reversing Cellular Dysfunction of Skin Repair Cells
    Article Snippet: the manufacturer’s protocol. Quantitative PCR was carried out using an ABIPRISM 7300 Sequence Detection System (Applied Biosystems, USA). HG-HUVECs and HG-HDFs were treated with NC mimic (HY- R04602 , MCE, USA), sEVs Hypo-5% + inhibitor NC (HY-RI04602, MCE, USA), sEVs Hypo-5% + inhibitor miR-21-5p (HY-RI00449, MCE), or miR-21-5p mimic (HY- R00449 , MCE). Total RNA was extracted using the same RNA e

    Wound Healing Assay:

    Article Title: Hypoxia-Challenged sEVs-Engineered Nanofiber Scaffolds Accelerate Diabetic Wound Healing via Reversing Cellular Dysfunction of Skin Repair Cells
    Article Snippet: Quantitative PCR was carried out using an ABIPRISM 7300 Sequence Detection System (Applied Biosystems, USA).Quantitative PCR was carried out using an ABIPRISM 7300 Sequence Detection System (Applied Biosystems, USA).. HG-HUVECs and HG-HDFs were treated with NC mimic (HY- R04602 , MCE, USA), sEVs Hypo-5% + inhibitor NC (HY-RI04602, MCE, USA), sEVs Hypo-5% + inhibitor miR-21-5p (HY-RI00449, MCE), or miR-21-5p mimic (HY- R00449 , MCE).. Total RNA was extracted using the same RNA extraction kit (YI SHAN Biotechnology, China).Total RNA was extracted using the same RNA extraction kit (YI SHAN Biotechnology, China).

    Article Title: Hypoxia-Challenged sEVs-Engineered Nanofiber Scaffolds Accelerate Diabetic Wound Healing via Reversing Cellular Dysfunction of Skin Repair Cells
    Article Snippet: the manufacturer’s protocol. Quantitative PCR was carried out using an ABIPRISM 7300 Sequence Detection System (Applied Biosystems, USA). HG-HUVECs and HG-HDFs were treated with NC mimic (HY- R04602 , MCE, USA), sEVs Hypo-5% + inhibitor NC (HY-RI04602, MCE, USA), sEVs Hypo-5% + inhibitor miR-21-5p (HY-RI00449, MCE), or miR-21-5p mimic (HY- R00449 , MCE). Total RNA was extracted using the same RNA e

    Tube Formation Assay:

    Article Title: Hypoxia-Challenged sEVs-Engineered Nanofiber Scaffolds Accelerate Diabetic Wound Healing via Reversing Cellular Dysfunction of Skin Repair Cells
    Article Snippet: Quantitative PCR was carried out using an ABIPRISM 7300 Sequence Detection System (Applied Biosystems, USA).Quantitative PCR was carried out using an ABIPRISM 7300 Sequence Detection System (Applied Biosystems, USA).. HG-HUVECs and HG-HDFs were treated with NC mimic (HY- R04602 , MCE, USA), sEVs Hypo-5% + inhibitor NC (HY-RI04602, MCE, USA), sEVs Hypo-5% + inhibitor miR-21-5p (HY-RI00449, MCE), or miR-21-5p mimic (HY- R00449 , MCE).. Total RNA was extracted using the same RNA extraction kit (YI SHAN Biotechnology, China).Total RNA was extracted using the same RNA extraction kit (YI SHAN Biotechnology, China).

    Article Title: Hypoxia-Challenged sEVs-Engineered Nanofiber Scaffolds Accelerate Diabetic Wound Healing via Reversing Cellular Dysfunction of Skin Repair Cells
    Article Snippet: the manufacturer’s protocol. Quantitative PCR was carried out using an ABIPRISM 7300 Sequence Detection System (Applied Biosystems, USA). HG-HUVECs and HG-HDFs were treated with NC mimic (HY- R04602 , MCE, USA), sEVs Hypo-5% + inhibitor NC (HY-RI04602, MCE, USA), sEVs Hypo-5% + inhibitor miR-21-5p (HY-RI00449, MCE), or miR-21-5p mimic (HY- R00449 , MCE). Total RNA was extracted using the same RNA e

    Transwell Migration Assay:

    Article Title: Hypoxia-Challenged sEVs-Engineered Nanofiber Scaffolds Accelerate Diabetic Wound Healing via Reversing Cellular Dysfunction of Skin Repair Cells
    Article Snippet: Quantitative PCR was carried out using an ABIPRISM 7300 Sequence Detection System (Applied Biosystems, USA).Quantitative PCR was carried out using an ABIPRISM 7300 Sequence Detection System (Applied Biosystems, USA).. HG-HUVECs and HG-HDFs were treated with NC mimic (HY- R04602 , MCE, USA), sEVs Hypo-5% + inhibitor NC (HY-RI04602, MCE, USA), sEVs Hypo-5% + inhibitor miR-21-5p (HY-RI00449, MCE), or miR-21-5p mimic (HY- R00449 , MCE).. Total RNA was extracted using the same RNA extraction kit (YI SHAN Biotechnology, China).Total RNA was extracted using the same RNA extraction kit (YI SHAN Biotechnology, China).

    Article Title: Hypoxia-Challenged sEVs-Engineered Nanofiber Scaffolds Accelerate Diabetic Wound Healing via Reversing Cellular Dysfunction of Skin Repair Cells
    Article Snippet: the manufacturer’s protocol. Quantitative PCR was carried out using an ABIPRISM 7300 Sequence Detection System (Applied Biosystems, USA). HG-HUVECs and HG-HDFs were treated with NC mimic (HY- R04602 , MCE, USA), sEVs Hypo-5% + inhibitor NC (HY-RI04602, MCE, USA), sEVs Hypo-5% + inhibitor miR-21-5p (HY-RI00449, MCE), or miR-21-5p mimic (HY- R00449 , MCE). Total RNA was extracted using the same RNA e

    Real-time Polymerase Chain Reaction:

    Article Title: Hypoxia-Challenged sEVs-Engineered Nanofiber Scaffolds Accelerate Diabetic Wound Healing via Reversing Cellular Dysfunction of Skin Repair Cells
    Article Snippet: Quantitative PCR was carried out using an ABIPRISM 7300 Sequence Detection System (Applied Biosystems, USA).Quantitative PCR was carried out using an ABIPRISM 7300 Sequence Detection System (Applied Biosystems, USA).. HG-HUVECs and HG-HDFs were treated with NC mimic (HY- R04602 , MCE, USA), sEVs Hypo-5% + inhibitor NC (HY-RI04602, MCE, USA), sEVs Hypo-5% + inhibitor miR-21-5p (HY-RI00449, MCE), or miR-21-5p mimic (HY- R00449 , MCE).. Total RNA was extracted using the same RNA extraction kit (YI SHAN Biotechnology, China).Total RNA was extracted using the same RNA extraction kit (YI SHAN Biotechnology, China).

    Article Title: Hypoxia-Challenged sEVs-Engineered Nanofiber Scaffolds Accelerate Diabetic Wound Healing via Reversing Cellular Dysfunction of Skin Repair Cells
    Article Snippet: the manufacturer’s protocol. Quantitative PCR was carried out using an ABIPRISM 7300 Sequence Detection System (Applied Biosystems, USA). HG-HUVECs and HG-HDFs were treated with NC mimic (HY- R04602 , MCE, USA), sEVs Hypo-5% + inhibitor NC (HY-RI04602, MCE, USA), sEVs Hypo-5% + inhibitor miR-21-5p (HY-RI00449, MCE), or miR-21-5p mimic (HY- R00449 , MCE). Total RNA was extracted using the same RNA e

    Polymerase Chain Reaction:

    Article Title: Hypoxia-Challenged sEVs-Engineered Nanofiber Scaffolds Accelerate Diabetic Wound Healing via Reversing Cellular Dysfunction of Skin Repair Cells
    Article Snippet: Quantitative PCR was carried out using an ABIPRISM 7300 Sequence Detection System (Applied Biosystems, USA).Quantitative PCR was carried out using an ABIPRISM 7300 Sequence Detection System (Applied Biosystems, USA).. HG-HUVECs and HG-HDFs were treated with NC mimic (HY- R04602 , MCE, USA), sEVs Hypo-5% + inhibitor NC (HY-RI04602, MCE, USA), sEVs Hypo-5% + inhibitor miR-21-5p (HY-RI00449, MCE), or miR-21-5p mimic (HY- R00449 , MCE).. Total RNA was extracted using the same RNA extraction kit (YI SHAN Biotechnology, China).Total RNA was extracted using the same RNA extraction kit (YI SHAN Biotechnology, China).

    Article Title: Hypoxia-Challenged sEVs-Engineered Nanofiber Scaffolds Accelerate Diabetic Wound Healing via Reversing Cellular Dysfunction of Skin Repair Cells
    Article Snippet: the manufacturer’s protocol. Quantitative PCR was carried out using an ABIPRISM 7300 Sequence Detection System (Applied Biosystems, USA). HG-HUVECs and HG-HDFs were treated with NC mimic (HY- R04602 , MCE, USA), sEVs Hypo-5% + inhibitor NC (HY-RI04602, MCE, USA), sEVs Hypo-5% + inhibitor miR-21-5p (HY-RI00449, MCE), or miR-21-5p mimic (HY- R00449 , MCE). Total RNA was extracted using the same RNA e

    RNA Extraction:

    Article Title: Hypoxia-Challenged sEVs-Engineered Nanofiber Scaffolds Accelerate Diabetic Wound Healing via Reversing Cellular Dysfunction of Skin Repair Cells
    Article Snippet: Quantitative PCR was carried out using an ABIPRISM 7300 Sequence Detection System (Applied Biosystems, USA).Quantitative PCR was carried out using an ABIPRISM 7300 Sequence Detection System (Applied Biosystems, USA).. HG-HUVECs and HG-HDFs were treated with NC mimic (HY- R04602 , MCE, USA), sEVs Hypo-5% + inhibitor NC (HY-RI04602, MCE, USA), sEVs Hypo-5% + inhibitor miR-21-5p (HY-RI00449, MCE), or miR-21-5p mimic (HY- R00449 , MCE).. Total RNA was extracted using the same RNA extraction kit (YI SHAN Biotechnology, China).Total RNA was extracted using the same RNA extraction kit (YI SHAN Biotechnology, China).

    Article Title: Hypoxia-Challenged sEVs-Engineered Nanofiber Scaffolds Accelerate Diabetic Wound Healing via Reversing Cellular Dysfunction of Skin Repair Cells
    Article Snippet: the manufacturer’s protocol. Quantitative PCR was carried out using an ABIPRISM 7300 Sequence Detection System (Applied Biosystems, USA). HG-HUVECs and HG-HDFs were treated with NC mimic (HY- R04602 , MCE, USA), sEVs Hypo-5% + inhibitor NC (HY-RI04602, MCE, USA), sEVs Hypo-5% + inhibitor miR-21-5p (HY-RI00449, MCE), or miR-21-5p mimic (HY- R00449 , MCE). Total RNA was extracted using the same RNA e

    Sequencing:

    Article Title: Hypoxia-Challenged sEVs-Engineered Nanofiber Scaffolds Accelerate Diabetic Wound Healing via Reversing Cellular Dysfunction of Skin Repair Cells
    Article Snippet: Quantitative PCR was carried out using an ABIPRISM 7300 Sequence Detection System (Applied Biosystems, USA).Quantitative PCR was carried out using an ABIPRISM 7300 Sequence Detection System (Applied Biosystems, USA).. HG-HUVECs and HG-HDFs were treated with NC mimic (HY- R04602 , MCE, USA), sEVs Hypo-5% + inhibitor NC (HY-RI04602, MCE, USA), sEVs Hypo-5% + inhibitor miR-21-5p (HY-RI00449, MCE), or miR-21-5p mimic (HY- R00449 , MCE).. Total RNA was extracted using the same RNA extraction kit (YI SHAN Biotechnology, China).Total RNA was extracted using the same RNA extraction kit (YI SHAN Biotechnology, China).

    Article Title: Hypoxia-Challenged sEVs-Engineered Nanofiber Scaffolds Accelerate Diabetic Wound Healing via Reversing Cellular Dysfunction of Skin Repair Cells
    Article Snippet: the manufacturer’s protocol. Quantitative PCR was carried out using an ABIPRISM 7300 Sequence Detection System (Applied Biosystems, USA). HG-HUVECs and HG-HDFs were treated with NC mimic (HY- R04602 , MCE, USA), sEVs Hypo-5% + inhibitor NC (HY-RI04602, MCE, USA), sEVs Hypo-5% + inhibitor miR-21-5p (HY-RI00449, MCE), or miR-21-5p mimic (HY- R00449 , MCE). Total RNA was extracted using the same RNA e

    Extraction:

    Article Title: Hypoxia-Challenged sEVs-Engineered Nanofiber Scaffolds Accelerate Diabetic Wound Healing via Reversing Cellular Dysfunction of Skin Repair Cells
    Article Snippet: Quantitative PCR was carried out using an ABIPRISM 7300 Sequence Detection System (Applied Biosystems, USA).Quantitative PCR was carried out using an ABIPRISM 7300 Sequence Detection System (Applied Biosystems, USA).. HG-HUVECs and HG-HDFs were treated with NC mimic (HY- R04602 , MCE, USA), sEVs Hypo-5% + inhibitor NC (HY-RI04602, MCE, USA), sEVs Hypo-5% + inhibitor miR-21-5p (HY-RI00449, MCE), or miR-21-5p mimic (HY- R00449 , MCE).. Total RNA was extracted using the same RNA extraction kit (YI SHAN Biotechnology, China).Total RNA was extracted using the same RNA extraction kit (YI SHAN Biotechnology, China).

    Article Title: Hypoxia-Challenged sEVs-Engineered Nanofiber Scaffolds Accelerate Diabetic Wound Healing via Reversing Cellular Dysfunction of Skin Repair Cells
    Article Snippet: the manufacturer’s protocol. Quantitative PCR was carried out using an ABIPRISM 7300 Sequence Detection System (Applied Biosystems, USA). HG-HUVECs and HG-HDFs were treated with NC mimic (HY- R04602 , MCE, USA), sEVs Hypo-5% + inhibitor NC (HY-RI04602, MCE, USA), sEVs Hypo-5% + inhibitor miR-21-5p (HY-RI00449, MCE), or miR-21-5p mimic (HY- R00449 , MCE). Total RNA was extracted using the same RNA e



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    mir  (MedChemExpress)
    94
    MedChemExpress mir
    <t>miR-21-5p</t> <t>is</t> <t>the</t> key mediator of sEVs Hypo-5% in recovering cellular function of HG-HUVECs and HG-HDFs. (A) Representative EdU images showing the proliferation of HG-HUVECs treated with NC mimic, sEVs Hypo-5% + inhibitor NC , sEVs Hypo-5% + inhibitor miR-21-5p , or miR-21-5p mimic. Scale bar = 100 μm. (B) Quantification of (A) ( n = 3 per group). (C) Scratch wound-healing assay of HG-HUVECs in different groups. Scale bar = 100 μm. (D) Quantification analysis of (C) ( n = 4 per group). (E) Matrigel tube-formation assay of HG-HUVECs after different treatments. Scale bar = 100 μm. (F) Quantification analysis of segments in (E) ( n = 3 per group). (G) Representative EdU images showing the proliferation of HG-HDFs treated with NC mimic, sEVs Hypo-5% + inhibitor NC , sEVs Hypo-5% + inhibitor miR-21-5p , or miR-21-5p mimic. Scale bar = 100 μm. (H) Quantification analysis of (G) ( n = 3 per group). (I) Scratch wound-healing assay of HG-HDFs. Scale bar = 100 μm. (J) Quantification analysis of (I) ( n = 3 per group). (K) Transwell migration assay of HG-HDFs after different treatments. Scale bar = 100 μm. (L) Quantification analysis of (K) ( n = 4 per group). ns, not significant; * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001.
    Mir, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/HY-R00449/hsa-miR-21-5p+mimic/pmc13150076-295-29-34
    Average 94 stars, based on 1 article reviews
    mir - by Bioz Stars, 2026-11
    94/100 stars
    		  Buy from Supplier

    Image Search Results


    miR-21-5p is the key mediator of sEVs Hypo-5% in recovering cellular function of HG-HUVECs and HG-HDFs. (A) Representative EdU images showing the proliferation of HG-HUVECs treated with NC mimic, sEVs Hypo-5% + inhibitor NC , sEVs Hypo-5% + inhibitor miR-21-5p , or miR-21-5p mimic. Scale bar = 100 μm. (B) Quantification of (A) ( n = 3 per group). (C) Scratch wound-healing assay of HG-HUVECs in different groups. Scale bar = 100 μm. (D) Quantification analysis of (C) ( n = 4 per group). (E) Matrigel tube-formation assay of HG-HUVECs after different treatments. Scale bar = 100 μm. (F) Quantification analysis of segments in (E) ( n = 3 per group). (G) Representative EdU images showing the proliferation of HG-HDFs treated with NC mimic, sEVs Hypo-5% + inhibitor NC , sEVs Hypo-5% + inhibitor miR-21-5p , or miR-21-5p mimic. Scale bar = 100 μm. (H) Quantification analysis of (G) ( n = 3 per group). (I) Scratch wound-healing assay of HG-HDFs. Scale bar = 100 μm. (J) Quantification analysis of (I) ( n = 3 per group). (K) Transwell migration assay of HG-HDFs after different treatments. Scale bar = 100 μm. (L) Quantification analysis of (K) ( n = 4 per group). ns, not significant; * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001.

    Journal: Research

    Article Title: Hypoxia-Challenged sEVs-Engineered Nanofiber Scaffolds Accelerate Diabetic Wound Healing via Reversing Cellular Dysfunction of Skin Repair Cells

    doi: 10.34133/research.1248

    Figure Lengend Snippet: miR-21-5p is the key mediator of sEVs Hypo-5% in recovering cellular function of HG-HUVECs and HG-HDFs. (A) Representative EdU images showing the proliferation of HG-HUVECs treated with NC mimic, sEVs Hypo-5% + inhibitor NC , sEVs Hypo-5% + inhibitor miR-21-5p , or miR-21-5p mimic. Scale bar = 100 μm. (B) Quantification of (A) ( n = 3 per group). (C) Scratch wound-healing assay of HG-HUVECs in different groups. Scale bar = 100 μm. (D) Quantification analysis of (C) ( n = 4 per group). (E) Matrigel tube-formation assay of HG-HUVECs after different treatments. Scale bar = 100 μm. (F) Quantification analysis of segments in (E) ( n = 3 per group). (G) Representative EdU images showing the proliferation of HG-HDFs treated with NC mimic, sEVs Hypo-5% + inhibitor NC , sEVs Hypo-5% + inhibitor miR-21-5p , or miR-21-5p mimic. Scale bar = 100 μm. (H) Quantification analysis of (G) ( n = 3 per group). (I) Scratch wound-healing assay of HG-HDFs. Scale bar = 100 μm. (J) Quantification analysis of (I) ( n = 3 per group). (K) Transwell migration assay of HG-HDFs after different treatments. Scale bar = 100 μm. (L) Quantification analysis of (K) ( n = 4 per group). ns, not significant; * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001.

    Article Snippet: HG-HUVECs and HG-HDFs were treated with NC mimic (HY- R04602 , MCE, USA), sEVs Hypo-5% + inhibitor NC (HY-RI04602, MCE, USA), sEVs Hypo-5% + inhibitor miR-21-5p (HY-RI00449, MCE), or miR-21-5p mimic (HY- R00449 , MCE).

    Techniques: Cell Function Assay, Wound Healing Assay, Tube Formation Assay, Transwell Migration Assay