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mir 199a 5p  (MedChemExpress)


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    Structured Review

    MedChemExpress mir 199a 5p
    Validation of the identified miRNAs in: ( I ) colon [(A) <t>mmu-miR-199a-3p;</t> (B) <t>mmu-miR-199a-5p;</t> (C) mmu-miR-21a-3p; (D) mmu-miR-21a-5p, (E) mmu-miR-34a-5p; (F) mmu-miR-126a-3p; (G) mmu-miR-126a-5p; (H) mmu-miR-7b-5p] and ( II ) small intestinal tissue [(A) mmu-miR-146a-5p; and (B) mmu-miR-142a-3p] of biotin-deficient and their pair-fed control mice. The levels of miRNA expression were analyzed using RNA samples extracted from mouse intestinal tissue by RT-qPCR analysis. Data are means ± SE of 4 pairs of animals (n = 4). All miRNA expression results were normalized relative to internal control miRNAs RNU1A1 and UniSp6, and comparison was made relative to simultaneously performed controls as described in Methods. Statistical significance was evaluated by the Student’s t -test using GraphPad Prism software. * p < 0.05; ** p < 0.01.
    Mir 199a 5p, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 94/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/HY-R00399/hsa-miR-199a-5p+mimic/pmc12943076-109-20-21
    Average 94 stars, based on 2 article reviews
    mir 199a 5p - by Bioz Stars, 2026-11
    94/100 stars

    Images

    1) Product Images from "Biotin Deficiency Alters the Expression Profile of Colonic microRNAs: Possible Contribution to the Alterations in Expression of Proteins Involved in the Maintenance of Colonic Physiology and Inflammation"

    Article Title: Biotin Deficiency Alters the Expression Profile of Colonic microRNAs: Possible Contribution to the Alterations in Expression of Proteins Involved in the Maintenance of Colonic Physiology and Inflammation

    Journal: Nutrients

    doi: 10.3390/nu18040612

    Validation of the identified miRNAs in: ( I ) colon [(A) mmu-miR-199a-3p; (B) mmu-miR-199a-5p; (C) mmu-miR-21a-3p; (D) mmu-miR-21a-5p, (E) mmu-miR-34a-5p; (F) mmu-miR-126a-3p; (G) mmu-miR-126a-5p; (H) mmu-miR-7b-5p] and ( II ) small intestinal tissue [(A) mmu-miR-146a-5p; and (B) mmu-miR-142a-3p] of biotin-deficient and their pair-fed control mice. The levels of miRNA expression were analyzed using RNA samples extracted from mouse intestinal tissue by RT-qPCR analysis. Data are means ± SE of 4 pairs of animals (n = 4). All miRNA expression results were normalized relative to internal control miRNAs RNU1A1 and UniSp6, and comparison was made relative to simultaneously performed controls as described in Methods. Statistical significance was evaluated by the Student’s t -test using GraphPad Prism software. * p < 0.05; ** p < 0.01.
    Figure Legend Snippet: Validation of the identified miRNAs in: ( I ) colon [(A) mmu-miR-199a-3p; (B) mmu-miR-199a-5p; (C) mmu-miR-21a-3p; (D) mmu-miR-21a-5p, (E) mmu-miR-34a-5p; (F) mmu-miR-126a-3p; (G) mmu-miR-126a-5p; (H) mmu-miR-7b-5p] and ( II ) small intestinal tissue [(A) mmu-miR-146a-5p; and (B) mmu-miR-142a-3p] of biotin-deficient and their pair-fed control mice. The levels of miRNA expression were analyzed using RNA samples extracted from mouse intestinal tissue by RT-qPCR analysis. Data are means ± SE of 4 pairs of animals (n = 4). All miRNA expression results were normalized relative to internal control miRNAs RNU1A1 and UniSp6, and comparison was made relative to simultaneously performed controls as described in Methods. Statistical significance was evaluated by the Student’s t -test using GraphPad Prism software. * p < 0.05; ** p < 0.01.

    Techniques Used: Biomarker Discovery, Control, Expressing, Quantitative RT-PCR, Comparison, Software

    Related Articles

    Transfection:

    Article Title: Biotin Deficiency Alters the Expression Profile of Colonic microRNAs: Possible Contribution to the Alterations in Expression of Proteins Involved in the Maintenance of Colonic Physiology and Inflammation
    Article Snippet: Human-derived colonic epithelial NCM460 cells were grown in Dulbecco’s modified Eagle medium (DMEM; Gibco, Waltham, MA, USA) with 20% (vol/vol) FBS and supplemented with streptomycin (100 μg/mL) and penicillin (100 U/mL) at 37 °C in 5% CO 2 —95% air environment in T-75 plastic flasks. .. Transient transfection of NCM460 cells was done using 200 nM miRNA mimic miR-190a-5p (MCE MedChemExpress, Cat# HY- R00361 ) and miR-199a-5p (MCE MedChemExpress, Cat# HY- R00399 ) with appropriate negative control miRNA mimic (MCE MedChemExpress, Cat# HY- R04602 ) for 48 h, using RNAiMax (Cat# 13778150) transfection reagent to achieve maximum transfection efficiency (Invitrogen, Carlsbad, CA, USA). .. After 48 h of post-transfection, the total RNA was isolated from the cells by using QIAzol reagent (QIAGEN) and RNeasy Kit (QIAGEN) following the manufacturer’s instructions.

    Article Title: Biotin Deficiency Alters the Expression Profile of Colonic microRNAs: Possible Contribution to the Alterations in Expression of Proteins Involved in the Maintenance of Colonic Physiology and Inflammation.
    Article Snippet: Human-derived colonic epithelial NCM460 cells were grown in Dulbecco’s modified Eagle medium (DMEM; Gibco, Waltham, MA, USA) with 20% (vol/vol) FBS and supplemented with streptomycin (100 μg/mL) and penicillin (100 U/mL) at 37 ◦C in 5% CO2—95% air environment in T-75 plastic flasks. .. Transient transfection of NCM460 cells was done using 200 nM miRNA mimic miR-190a-5p (MCE MedChemExpress, Cat# HYR00361) and miR-199a-5p (MCE MedChemExpress, Cat# HY-R00399) with appropriate negative control miRNA mimic (MCE MedChemExpress, Cat# HY-R04602) for 48 h, using RNAiMax (Cat# 13778150) transfection reagent to achieve maximum transfection efficiency (Invitrogen, Carlsbad, CA, USA). .. After 48 h of post-transfection, the total RNA was isolated from the cells by using QIAzol reagent (QIAGEN) and RNeasy Kit (QIAGEN) following the manufacturer’s instructions.

    Negative Control:

    Article Title: Biotin Deficiency Alters the Expression Profile of Colonic microRNAs: Possible Contribution to the Alterations in Expression of Proteins Involved in the Maintenance of Colonic Physiology and Inflammation
    Article Snippet: Human-derived colonic epithelial NCM460 cells were grown in Dulbecco’s modified Eagle medium (DMEM; Gibco, Waltham, MA, USA) with 20% (vol/vol) FBS and supplemented with streptomycin (100 μg/mL) and penicillin (100 U/mL) at 37 °C in 5% CO 2 —95% air environment in T-75 plastic flasks. .. Transient transfection of NCM460 cells was done using 200 nM miRNA mimic miR-190a-5p (MCE MedChemExpress, Cat# HY- R00361 ) and miR-199a-5p (MCE MedChemExpress, Cat# HY- R00399 ) with appropriate negative control miRNA mimic (MCE MedChemExpress, Cat# HY- R04602 ) for 48 h, using RNAiMax (Cat# 13778150) transfection reagent to achieve maximum transfection efficiency (Invitrogen, Carlsbad, CA, USA). .. After 48 h of post-transfection, the total RNA was isolated from the cells by using QIAzol reagent (QIAGEN) and RNeasy Kit (QIAGEN) following the manufacturer’s instructions.

    Article Title: Biotin Deficiency Alters the Expression Profile of Colonic microRNAs: Possible Contribution to the Alterations in Expression of Proteins Involved in the Maintenance of Colonic Physiology and Inflammation.
    Article Snippet: Human-derived colonic epithelial NCM460 cells were grown in Dulbecco’s modified Eagle medium (DMEM; Gibco, Waltham, MA, USA) with 20% (vol/vol) FBS and supplemented with streptomycin (100 μg/mL) and penicillin (100 U/mL) at 37 ◦C in 5% CO2—95% air environment in T-75 plastic flasks. .. Transient transfection of NCM460 cells was done using 200 nM miRNA mimic miR-190a-5p (MCE MedChemExpress, Cat# HYR00361) and miR-199a-5p (MCE MedChemExpress, Cat# HY-R00399) with appropriate negative control miRNA mimic (MCE MedChemExpress, Cat# HY-R04602) for 48 h, using RNAiMax (Cat# 13778150) transfection reagent to achieve maximum transfection efficiency (Invitrogen, Carlsbad, CA, USA). .. After 48 h of post-transfection, the total RNA was isolated from the cells by using QIAzol reagent (QIAGEN) and RNeasy Kit (QIAGEN) following the manufacturer’s instructions.



    Similar Products

    mir 199a 5p  (MedChemExpress)
    94
    MedChemExpress mir 199a 5p
    Validation of the identified miRNAs in: ( I ) colon [(A) <t>mmu-miR-199a-3p;</t> (B) <t>mmu-miR-199a-5p;</t> (C) mmu-miR-21a-3p; (D) mmu-miR-21a-5p, (E) mmu-miR-34a-5p; (F) mmu-miR-126a-3p; (G) mmu-miR-126a-5p; (H) mmu-miR-7b-5p] and ( II ) small intestinal tissue [(A) mmu-miR-146a-5p; and (B) mmu-miR-142a-3p] of biotin-deficient and their pair-fed control mice. The levels of miRNA expression were analyzed using RNA samples extracted from mouse intestinal tissue by RT-qPCR analysis. Data are means ± SE of 4 pairs of animals (n = 4). All miRNA expression results were normalized relative to internal control miRNAs RNU1A1 and UniSp6, and comparison was made relative to simultaneously performed controls as described in Methods. Statistical significance was evaluated by the Student’s t -test using GraphPad Prism software. * p < 0.05; ** p < 0.01.
    Mir 199a 5p, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/HY-R00399/hsa-miR-199a-5p+mimic/pmc12943076-109-20-21
    Average 94 stars, based on 1 article reviews
    mir 199a 5p - by Bioz Stars, 2026-11
    94/100 stars
    		  Buy from Supplier

    Image Search Results


    Validation of the identified miRNAs in: ( I ) colon [(A) mmu-miR-199a-3p; (B) mmu-miR-199a-5p; (C) mmu-miR-21a-3p; (D) mmu-miR-21a-5p, (E) mmu-miR-34a-5p; (F) mmu-miR-126a-3p; (G) mmu-miR-126a-5p; (H) mmu-miR-7b-5p] and ( II ) small intestinal tissue [(A) mmu-miR-146a-5p; and (B) mmu-miR-142a-3p] of biotin-deficient and their pair-fed control mice. The levels of miRNA expression were analyzed using RNA samples extracted from mouse intestinal tissue by RT-qPCR analysis. Data are means ± SE of 4 pairs of animals (n = 4). All miRNA expression results were normalized relative to internal control miRNAs RNU1A1 and UniSp6, and comparison was made relative to simultaneously performed controls as described in Methods. Statistical significance was evaluated by the Student’s t -test using GraphPad Prism software. * p < 0.05; ** p < 0.01.

    Journal: Nutrients

    Article Title: Biotin Deficiency Alters the Expression Profile of Colonic microRNAs: Possible Contribution to the Alterations in Expression of Proteins Involved in the Maintenance of Colonic Physiology and Inflammation

    doi: 10.3390/nu18040612

    Figure Lengend Snippet: Validation of the identified miRNAs in: ( I ) colon [(A) mmu-miR-199a-3p; (B) mmu-miR-199a-5p; (C) mmu-miR-21a-3p; (D) mmu-miR-21a-5p, (E) mmu-miR-34a-5p; (F) mmu-miR-126a-3p; (G) mmu-miR-126a-5p; (H) mmu-miR-7b-5p] and ( II ) small intestinal tissue [(A) mmu-miR-146a-5p; and (B) mmu-miR-142a-3p] of biotin-deficient and their pair-fed control mice. The levels of miRNA expression were analyzed using RNA samples extracted from mouse intestinal tissue by RT-qPCR analysis. Data are means ± SE of 4 pairs of animals (n = 4). All miRNA expression results were normalized relative to internal control miRNAs RNU1A1 and UniSp6, and comparison was made relative to simultaneously performed controls as described in Methods. Statistical significance was evaluated by the Student’s t -test using GraphPad Prism software. * p < 0.05; ** p < 0.01.

    Article Snippet: Transient transfection of NCM460 cells was done using 200 nM miRNA mimic miR-190a-5p (MCE MedChemExpress, Cat# HY- R00361 ) and miR-199a-5p (MCE MedChemExpress, Cat# HY- R00399 ) with appropriate negative control miRNA mimic (MCE MedChemExpress, Cat# HY- R04602 ) for 48 h, using RNAiMax (Cat# 13778150) transfection reagent to achieve maximum transfection efficiency (Invitrogen, Carlsbad, CA, USA).

    Techniques: Biomarker Discovery, Control, Expressing, Quantitative RT-PCR, Comparison, Software