bavachin  (MedChemExpress)


Bioz Verified Symbol MedChemExpress is a verified supplier
Bioz Manufacturer Symbol MedChemExpress manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 93

    Structured Review

    MedChemExpress bavachin
    <t>Bavachin</t> induces GSH depletion and lipid peroxidation accumulation in osteosarcoma (OS) cells. (a, b) Bavachin induces GSH depletion in a dose-related manner over 24 h. (c, d) Bavachin greatly induces ROS generation in OS cells over 24 h. (e, f) Bavachin increases MDA accumulation in a dose-related manner. (g, h) Western blotting analysis shows the expression of p-STAT3, STAT3, P53, SLC7A11, and GPX4 in OS cells treated with bavachin (10 μ M, 20 μ M, and 40 μ M) for 24 h. p
    Bavachin, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/bavachin/product/MedChemExpress
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    bavachin - by Bioz Stars, 2022-07
    93/100 stars

    Images

    1) Product Images from "Bavachin Induces Ferroptosis through the STAT3/P53/SLC7A11 Axis in Osteosarcoma Cells"

    Article Title: Bavachin Induces Ferroptosis through the STAT3/P53/SLC7A11 Axis in Osteosarcoma Cells

    Journal: Oxidative Medicine and Cellular Longevity

    doi: 10.1155/2021/1783485

    Bavachin induces GSH depletion and lipid peroxidation accumulation in osteosarcoma (OS) cells. (a, b) Bavachin induces GSH depletion in a dose-related manner over 24 h. (c, d) Bavachin greatly induces ROS generation in OS cells over 24 h. (e, f) Bavachin increases MDA accumulation in a dose-related manner. (g, h) Western blotting analysis shows the expression of p-STAT3, STAT3, P53, SLC7A11, and GPX4 in OS cells treated with bavachin (10 μ M, 20 μ M, and 40 μ M) for 24 h. p
    Figure Legend Snippet: Bavachin induces GSH depletion and lipid peroxidation accumulation in osteosarcoma (OS) cells. (a, b) Bavachin induces GSH depletion in a dose-related manner over 24 h. (c, d) Bavachin greatly induces ROS generation in OS cells over 24 h. (e, f) Bavachin increases MDA accumulation in a dose-related manner. (g, h) Western blotting analysis shows the expression of p-STAT3, STAT3, P53, SLC7A11, and GPX4 in OS cells treated with bavachin (10 μ M, 20 μ M, and 40 μ M) for 24 h. p

    Techniques Used: Multiple Displacement Amplification, Western Blot, Expressing

    SLC7A11 overexpression alleviates bavachin-induced ferroptosis in osteosarcoma (OS) cells. OS cells were transfected with a SLC7A11 overexpressing plasmid and treated with bavachin (40 μ M, 24 h). (a, b) Western blotting demonstrates the relative levels of SLC7A11 and GPX4 protein expression. (c) Overexpression of SLC7A11 restores bavachin-induced GSH depletion in OS cells. (d, e) Overexpression of SLC7A11 reduces bavachin-induced ROS and MDA accumulation. (f) Overexpression of SLC7A11 recovers bavachin-induced ferroptosis in OS cells. ∗ p
    Figure Legend Snippet: SLC7A11 overexpression alleviates bavachin-induced ferroptosis in osteosarcoma (OS) cells. OS cells were transfected with a SLC7A11 overexpressing plasmid and treated with bavachin (40 μ M, 24 h). (a, b) Western blotting demonstrates the relative levels of SLC7A11 and GPX4 protein expression. (c) Overexpression of SLC7A11 restores bavachin-induced GSH depletion in OS cells. (d, e) Overexpression of SLC7A11 reduces bavachin-induced ROS and MDA accumulation. (f) Overexpression of SLC7A11 recovers bavachin-induced ferroptosis in OS cells. ∗ p

    Techniques Used: Over Expression, Transfection, Plasmid Preparation, Western Blot, Expressing, Multiple Displacement Amplification

    Bavachin-induced ferrous iron accumulation initiates death of osteosarcoma (OS) cells. (a) Bavachin (24 h) induced ferrous iron accumulation in OS cells occurs in a concentration-dependent manner. (b) Deferoxamine (DFO) rescues cells from bavachin-induced cell death. (c, d) Western blotting analysis shows the protein levels of transferrin receptor (TFRC), divalent metal transporter-1 (DMT1), ferritin light chain (FTH), and ferritin heavy chain (FTL) in MG63 and HOS cells treated with bavachin (10 μ M, 20 μ M, and 40 μ M) for 24 h. ∗ p
    Figure Legend Snippet: Bavachin-induced ferrous iron accumulation initiates death of osteosarcoma (OS) cells. (a) Bavachin (24 h) induced ferrous iron accumulation in OS cells occurs in a concentration-dependent manner. (b) Deferoxamine (DFO) rescues cells from bavachin-induced cell death. (c, d) Western blotting analysis shows the protein levels of transferrin receptor (TFRC), divalent metal transporter-1 (DMT1), ferritin light chain (FTH), and ferritin heavy chain (FTL) in MG63 and HOS cells treated with bavachin (10 μ M, 20 μ M, and 40 μ M) for 24 h. ∗ p

    Techniques Used: Concentration Assay, Western Blot

    p-STAT3 activation downregulates P53 expression to rescue bavachin-induced ferroptosis in osteosarcoma (OS) cells. (a, b) Western blotting shows the relative levels of p-STAT3, STAT3, P53, and GPX4 protein expression. (c) p-STAT3 activation restores bavachin-induced GSH depletion. (d, e) p-STAT3 activation reduces bavachin-induced ROS and MDA accumulation. (f) p-STAT3 activation rescues bavachin-induced ferroptosis in OS cells. ∗ p
    Figure Legend Snippet: p-STAT3 activation downregulates P53 expression to rescue bavachin-induced ferroptosis in osteosarcoma (OS) cells. (a, b) Western blotting shows the relative levels of p-STAT3, STAT3, P53, and GPX4 protein expression. (c) p-STAT3 activation restores bavachin-induced GSH depletion. (d, e) p-STAT3 activation reduces bavachin-induced ROS and MDA accumulation. (f) p-STAT3 activation rescues bavachin-induced ferroptosis in OS cells. ∗ p

    Techniques Used: Activation Assay, Expressing, Western Blot, Multiple Displacement Amplification

    Schematic diagram displays potential mechanism of bavachin-induced ferroptosis in osteosarcoma cells. LP: lipid peroxidation.
    Figure Legend Snippet: Schematic diagram displays potential mechanism of bavachin-induced ferroptosis in osteosarcoma cells. LP: lipid peroxidation.

    Techniques Used:

    Bavachin induces ferroptosis in osteosarcoma (OS) cells. (a) The ultrastructure of MG63 and HOS cells was observed using transmission electronic microscopy. Cells in the bavachin (40 μ M, 24 h) group display shrinking mitochondria, higher density of the mitochondrial membrane, and reduction or disappearance of the mitochondrial cristae. (b) Merged images of JC-1 staining demonstrate the change in the mitochondrial membrane potential (MMP) (red: aggregates and high MMP; green: monomers and low MMP). (c) CCK8 assay shows that deferoxamine (DFO), ferrostatin-1 (Fer-1), and liproxstatin-1 (Lip-1) rescue bavachin-induced cell death in OS cells. ∗∗∗ p
    Figure Legend Snippet: Bavachin induces ferroptosis in osteosarcoma (OS) cells. (a) The ultrastructure of MG63 and HOS cells was observed using transmission electronic microscopy. Cells in the bavachin (40 μ M, 24 h) group display shrinking mitochondria, higher density of the mitochondrial membrane, and reduction or disappearance of the mitochondrial cristae. (b) Merged images of JC-1 staining demonstrate the change in the mitochondrial membrane potential (MMP) (red: aggregates and high MMP; green: monomers and low MMP). (c) CCK8 assay shows that deferoxamine (DFO), ferrostatin-1 (Fer-1), and liproxstatin-1 (Lip-1) rescue bavachin-induced cell death in OS cells. ∗∗∗ p

    Techniques Used: Transmission Assay, Microscopy, Staining, CCK-8 Assay

    Bavachin inhibits osteosarcoma (OS) cell viability. (a) Chemical structure of bavachin. (b) CCK8 assay shows bavachin inhibits OS cells in a concentration- (5 μ M, 10 μ M, 20 μ M, 40 μ M, and 80 μ M) and time- (24 h, 48 h, and 72 h) dependent manner. (c) Morphologic features of OS cells on microscopy. Cells became round and showed shrunk after bavachin (10 μ M, 20 μ M, and 40 μ M) treatment for 24 h.
    Figure Legend Snippet: Bavachin inhibits osteosarcoma (OS) cell viability. (a) Chemical structure of bavachin. (b) CCK8 assay shows bavachin inhibits OS cells in a concentration- (5 μ M, 10 μ M, 20 μ M, 40 μ M, and 80 μ M) and time- (24 h, 48 h, and 72 h) dependent manner. (c) Morphologic features of OS cells on microscopy. Cells became round and showed shrunk after bavachin (10 μ M, 20 μ M, and 40 μ M) treatment for 24 h.

    Techniques Used: CCK-8 Assay, Concentration Assay, Microscopy

    P53 inactivation upregulates SLC7A11 to alleviate bavachin-induced ferroptosis in osteosarcoma (OS) cells. OS cells were pretreated with pifithrin- α (PFT- α , 5 μ M) followed by treatment with bavachin (40 μ M, 24 h). (a, b) Western blotting shows the relative levels of P53, SLC7A11, and GPX4 protein expression. (c) PFT- α (5 μ M) restores bavachin-induced GSH depletion. (d, e) PFT- α reduces bavachin-induced ROS and MDA accumulation. (f) PFT- α recovers bavachin-induced ferroptosis in OS cells. ∗ p
    Figure Legend Snippet: P53 inactivation upregulates SLC7A11 to alleviate bavachin-induced ferroptosis in osteosarcoma (OS) cells. OS cells were pretreated with pifithrin- α (PFT- α , 5 μ M) followed by treatment with bavachin (40 μ M, 24 h). (a, b) Western blotting shows the relative levels of P53, SLC7A11, and GPX4 protein expression. (c) PFT- α (5 μ M) restores bavachin-induced GSH depletion. (d, e) PFT- α reduces bavachin-induced ROS and MDA accumulation. (f) PFT- α recovers bavachin-induced ferroptosis in OS cells. ∗ p

    Techniques Used: Western Blot, Expressing, Multiple Displacement Amplification

    Similar Products

  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 93
    MedChemExpress bavachin
    <t>Bavachin</t> induces GSH depletion and lipid peroxidation accumulation in osteosarcoma (OS) cells. (a, b) Bavachin induces GSH depletion in a dose-related manner over 24 h. (c, d) Bavachin greatly induces ROS generation in OS cells over 24 h. (e, f) Bavachin increases MDA accumulation in a dose-related manner. (g, h) Western blotting analysis shows the expression of p-STAT3, STAT3, P53, SLC7A11, and GPX4 in OS cells treated with bavachin (10 μ M, 20 μ M, and 40 μ M) for 24 h. p
    Bavachin, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/bavachin/product/MedChemExpress
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    bavachin - by Bioz Stars, 2022-07
    93/100 stars
      Buy from Supplier

    Image Search Results


    Bavachin induces GSH depletion and lipid peroxidation accumulation in osteosarcoma (OS) cells. (a, b) Bavachin induces GSH depletion in a dose-related manner over 24 h. (c, d) Bavachin greatly induces ROS generation in OS cells over 24 h. (e, f) Bavachin increases MDA accumulation in a dose-related manner. (g, h) Western blotting analysis shows the expression of p-STAT3, STAT3, P53, SLC7A11, and GPX4 in OS cells treated with bavachin (10 μ M, 20 μ M, and 40 μ M) for 24 h. p

    Journal: Oxidative Medicine and Cellular Longevity

    Article Title: Bavachin Induces Ferroptosis through the STAT3/P53/SLC7A11 Axis in Osteosarcoma Cells

    doi: 10.1155/2021/1783485

    Figure Lengend Snippet: Bavachin induces GSH depletion and lipid peroxidation accumulation in osteosarcoma (OS) cells. (a, b) Bavachin induces GSH depletion in a dose-related manner over 24 h. (c, d) Bavachin greatly induces ROS generation in OS cells over 24 h. (e, f) Bavachin increases MDA accumulation in a dose-related manner. (g, h) Western blotting analysis shows the expression of p-STAT3, STAT3, P53, SLC7A11, and GPX4 in OS cells treated with bavachin (10 μ M, 20 μ M, and 40 μ M) for 24 h. p

    Article Snippet: Bavachin was purchased from MCE (China), and pifithrin-α (PFT-α), deferoxamine (DFO), ferrostatin-1 (Fer-1), and liproxstatin-1 (Lip-1) were purchased from Topscience (China).

    Techniques: Multiple Displacement Amplification, Western Blot, Expressing

    SLC7A11 overexpression alleviates bavachin-induced ferroptosis in osteosarcoma (OS) cells. OS cells were transfected with a SLC7A11 overexpressing plasmid and treated with bavachin (40 μ M, 24 h). (a, b) Western blotting demonstrates the relative levels of SLC7A11 and GPX4 protein expression. (c) Overexpression of SLC7A11 restores bavachin-induced GSH depletion in OS cells. (d, e) Overexpression of SLC7A11 reduces bavachin-induced ROS and MDA accumulation. (f) Overexpression of SLC7A11 recovers bavachin-induced ferroptosis in OS cells. ∗ p

    Journal: Oxidative Medicine and Cellular Longevity

    Article Title: Bavachin Induces Ferroptosis through the STAT3/P53/SLC7A11 Axis in Osteosarcoma Cells

    doi: 10.1155/2021/1783485

    Figure Lengend Snippet: SLC7A11 overexpression alleviates bavachin-induced ferroptosis in osteosarcoma (OS) cells. OS cells were transfected with a SLC7A11 overexpressing plasmid and treated with bavachin (40 μ M, 24 h). (a, b) Western blotting demonstrates the relative levels of SLC7A11 and GPX4 protein expression. (c) Overexpression of SLC7A11 restores bavachin-induced GSH depletion in OS cells. (d, e) Overexpression of SLC7A11 reduces bavachin-induced ROS and MDA accumulation. (f) Overexpression of SLC7A11 recovers bavachin-induced ferroptosis in OS cells. ∗ p

    Article Snippet: Bavachin was purchased from MCE (China), and pifithrin-α (PFT-α), deferoxamine (DFO), ferrostatin-1 (Fer-1), and liproxstatin-1 (Lip-1) were purchased from Topscience (China).

    Techniques: Over Expression, Transfection, Plasmid Preparation, Western Blot, Expressing, Multiple Displacement Amplification

    Bavachin-induced ferrous iron accumulation initiates death of osteosarcoma (OS) cells. (a) Bavachin (24 h) induced ferrous iron accumulation in OS cells occurs in a concentration-dependent manner. (b) Deferoxamine (DFO) rescues cells from bavachin-induced cell death. (c, d) Western blotting analysis shows the protein levels of transferrin receptor (TFRC), divalent metal transporter-1 (DMT1), ferritin light chain (FTH), and ferritin heavy chain (FTL) in MG63 and HOS cells treated with bavachin (10 μ M, 20 μ M, and 40 μ M) for 24 h. ∗ p

    Journal: Oxidative Medicine and Cellular Longevity

    Article Title: Bavachin Induces Ferroptosis through the STAT3/P53/SLC7A11 Axis in Osteosarcoma Cells

    doi: 10.1155/2021/1783485

    Figure Lengend Snippet: Bavachin-induced ferrous iron accumulation initiates death of osteosarcoma (OS) cells. (a) Bavachin (24 h) induced ferrous iron accumulation in OS cells occurs in a concentration-dependent manner. (b) Deferoxamine (DFO) rescues cells from bavachin-induced cell death. (c, d) Western blotting analysis shows the protein levels of transferrin receptor (TFRC), divalent metal transporter-1 (DMT1), ferritin light chain (FTH), and ferritin heavy chain (FTL) in MG63 and HOS cells treated with bavachin (10 μ M, 20 μ M, and 40 μ M) for 24 h. ∗ p

    Article Snippet: Bavachin was purchased from MCE (China), and pifithrin-α (PFT-α), deferoxamine (DFO), ferrostatin-1 (Fer-1), and liproxstatin-1 (Lip-1) were purchased from Topscience (China).

    Techniques: Concentration Assay, Western Blot

    p-STAT3 activation downregulates P53 expression to rescue bavachin-induced ferroptosis in osteosarcoma (OS) cells. (a, b) Western blotting shows the relative levels of p-STAT3, STAT3, P53, and GPX4 protein expression. (c) p-STAT3 activation restores bavachin-induced GSH depletion. (d, e) p-STAT3 activation reduces bavachin-induced ROS and MDA accumulation. (f) p-STAT3 activation rescues bavachin-induced ferroptosis in OS cells. ∗ p

    Journal: Oxidative Medicine and Cellular Longevity

    Article Title: Bavachin Induces Ferroptosis through the STAT3/P53/SLC7A11 Axis in Osteosarcoma Cells

    doi: 10.1155/2021/1783485

    Figure Lengend Snippet: p-STAT3 activation downregulates P53 expression to rescue bavachin-induced ferroptosis in osteosarcoma (OS) cells. (a, b) Western blotting shows the relative levels of p-STAT3, STAT3, P53, and GPX4 protein expression. (c) p-STAT3 activation restores bavachin-induced GSH depletion. (d, e) p-STAT3 activation reduces bavachin-induced ROS and MDA accumulation. (f) p-STAT3 activation rescues bavachin-induced ferroptosis in OS cells. ∗ p

    Article Snippet: Bavachin was purchased from MCE (China), and pifithrin-α (PFT-α), deferoxamine (DFO), ferrostatin-1 (Fer-1), and liproxstatin-1 (Lip-1) were purchased from Topscience (China).

    Techniques: Activation Assay, Expressing, Western Blot, Multiple Displacement Amplification

    Schematic diagram displays potential mechanism of bavachin-induced ferroptosis in osteosarcoma cells. LP: lipid peroxidation.

    Journal: Oxidative Medicine and Cellular Longevity

    Article Title: Bavachin Induces Ferroptosis through the STAT3/P53/SLC7A11 Axis in Osteosarcoma Cells

    doi: 10.1155/2021/1783485

    Figure Lengend Snippet: Schematic diagram displays potential mechanism of bavachin-induced ferroptosis in osteosarcoma cells. LP: lipid peroxidation.

    Article Snippet: Bavachin was purchased from MCE (China), and pifithrin-α (PFT-α), deferoxamine (DFO), ferrostatin-1 (Fer-1), and liproxstatin-1 (Lip-1) were purchased from Topscience (China).

    Techniques:

    Bavachin induces ferroptosis in osteosarcoma (OS) cells. (a) The ultrastructure of MG63 and HOS cells was observed using transmission electronic microscopy. Cells in the bavachin (40 μ M, 24 h) group display shrinking mitochondria, higher density of the mitochondrial membrane, and reduction or disappearance of the mitochondrial cristae. (b) Merged images of JC-1 staining demonstrate the change in the mitochondrial membrane potential (MMP) (red: aggregates and high MMP; green: monomers and low MMP). (c) CCK8 assay shows that deferoxamine (DFO), ferrostatin-1 (Fer-1), and liproxstatin-1 (Lip-1) rescue bavachin-induced cell death in OS cells. ∗∗∗ p

    Journal: Oxidative Medicine and Cellular Longevity

    Article Title: Bavachin Induces Ferroptosis through the STAT3/P53/SLC7A11 Axis in Osteosarcoma Cells

    doi: 10.1155/2021/1783485

    Figure Lengend Snippet: Bavachin induces ferroptosis in osteosarcoma (OS) cells. (a) The ultrastructure of MG63 and HOS cells was observed using transmission electronic microscopy. Cells in the bavachin (40 μ M, 24 h) group display shrinking mitochondria, higher density of the mitochondrial membrane, and reduction or disappearance of the mitochondrial cristae. (b) Merged images of JC-1 staining demonstrate the change in the mitochondrial membrane potential (MMP) (red: aggregates and high MMP; green: monomers and low MMP). (c) CCK8 assay shows that deferoxamine (DFO), ferrostatin-1 (Fer-1), and liproxstatin-1 (Lip-1) rescue bavachin-induced cell death in OS cells. ∗∗∗ p

    Article Snippet: Bavachin was purchased from MCE (China), and pifithrin-α (PFT-α), deferoxamine (DFO), ferrostatin-1 (Fer-1), and liproxstatin-1 (Lip-1) were purchased from Topscience (China).

    Techniques: Transmission Assay, Microscopy, Staining, CCK-8 Assay

    Bavachin inhibits osteosarcoma (OS) cell viability. (a) Chemical structure of bavachin. (b) CCK8 assay shows bavachin inhibits OS cells in a concentration- (5 μ M, 10 μ M, 20 μ M, 40 μ M, and 80 μ M) and time- (24 h, 48 h, and 72 h) dependent manner. (c) Morphologic features of OS cells on microscopy. Cells became round and showed shrunk after bavachin (10 μ M, 20 μ M, and 40 μ M) treatment for 24 h.

    Journal: Oxidative Medicine and Cellular Longevity

    Article Title: Bavachin Induces Ferroptosis through the STAT3/P53/SLC7A11 Axis in Osteosarcoma Cells

    doi: 10.1155/2021/1783485

    Figure Lengend Snippet: Bavachin inhibits osteosarcoma (OS) cell viability. (a) Chemical structure of bavachin. (b) CCK8 assay shows bavachin inhibits OS cells in a concentration- (5 μ M, 10 μ M, 20 μ M, 40 μ M, and 80 μ M) and time- (24 h, 48 h, and 72 h) dependent manner. (c) Morphologic features of OS cells on microscopy. Cells became round and showed shrunk after bavachin (10 μ M, 20 μ M, and 40 μ M) treatment for 24 h.

    Article Snippet: Bavachin was purchased from MCE (China), and pifithrin-α (PFT-α), deferoxamine (DFO), ferrostatin-1 (Fer-1), and liproxstatin-1 (Lip-1) were purchased from Topscience (China).

    Techniques: CCK-8 Assay, Concentration Assay, Microscopy

    P53 inactivation upregulates SLC7A11 to alleviate bavachin-induced ferroptosis in osteosarcoma (OS) cells. OS cells were pretreated with pifithrin- α (PFT- α , 5 μ M) followed by treatment with bavachin (40 μ M, 24 h). (a, b) Western blotting shows the relative levels of P53, SLC7A11, and GPX4 protein expression. (c) PFT- α (5 μ M) restores bavachin-induced GSH depletion. (d, e) PFT- α reduces bavachin-induced ROS and MDA accumulation. (f) PFT- α recovers bavachin-induced ferroptosis in OS cells. ∗ p

    Journal: Oxidative Medicine and Cellular Longevity

    Article Title: Bavachin Induces Ferroptosis through the STAT3/P53/SLC7A11 Axis in Osteosarcoma Cells

    doi: 10.1155/2021/1783485

    Figure Lengend Snippet: P53 inactivation upregulates SLC7A11 to alleviate bavachin-induced ferroptosis in osteosarcoma (OS) cells. OS cells were pretreated with pifithrin- α (PFT- α , 5 μ M) followed by treatment with bavachin (40 μ M, 24 h). (a, b) Western blotting shows the relative levels of P53, SLC7A11, and GPX4 protein expression. (c) PFT- α (5 μ M) restores bavachin-induced GSH depletion. (d, e) PFT- α reduces bavachin-induced ROS and MDA accumulation. (f) PFT- α recovers bavachin-induced ferroptosis in OS cells. ∗ p

    Article Snippet: Bavachin was purchased from MCE (China), and pifithrin-α (PFT-α), deferoxamine (DFO), ferrostatin-1 (Fer-1), and liproxstatin-1 (Lip-1) were purchased from Topscience (China).

    Techniques: Western Blot, Expressing, Multiple Displacement Amplification