cisplatin  (MedChemExpress)


Bioz Verified Symbol MedChemExpress is a verified supplier
Bioz Manufacturer Symbol MedChemExpress manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 94

    Structured Review

    MedChemExpress cisplatin
    HVEM-overexpressed MSCs promote the capacity of chemoresistance in cholangiocarcinoma cells. (A) HVEM expression was detected by western blotting in MSCs. Ov-HVEM, HVEM overexpression. (B-E) CCK-8 assays were employed to examine the cell viability in RBE and QBC939 cell lines co-cultured with MSCs, and 5-FU and <t>cisplatin</t> were added to the culture system. *P
    Cisplatin, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/cisplatin/product/MedChemExpress
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    cisplatin - by Bioz Stars, 2022-05
    94/100 stars

    Images

    1) Product Images from "Mesenchymal stem cells promote chemoresistance by activating autophagy in intrahepatic cholangiocarcinoma"

    Article Title: Mesenchymal stem cells promote chemoresistance by activating autophagy in intrahepatic cholangiocarcinoma

    Journal: Oncology Reports

    doi: 10.3892/or.2020.7838

    HVEM-overexpressed MSCs promote the capacity of chemoresistance in cholangiocarcinoma cells. (A) HVEM expression was detected by western blotting in MSCs. Ov-HVEM, HVEM overexpression. (B-E) CCK-8 assays were employed to examine the cell viability in RBE and QBC939 cell lines co-cultured with MSCs, and 5-FU and cisplatin were added to the culture system. *P
    Figure Legend Snippet: HVEM-overexpressed MSCs promote the capacity of chemoresistance in cholangiocarcinoma cells. (A) HVEM expression was detected by western blotting in MSCs. Ov-HVEM, HVEM overexpression. (B-E) CCK-8 assays were employed to examine the cell viability in RBE and QBC939 cell lines co-cultured with MSCs, and 5-FU and cisplatin were added to the culture system. *P

    Techniques Used: Expressing, Western Blot, Over Expression, CCK-8 Assay, Cell Culture

    IL-6 promotes the chemoresistance of cholangiocarcinoma cells through activation of autophagy. (A) The expression of LC3 and p62 was detected by western blotting in the RBE cell line with treatment of IL-6. (The Control a group was the RBE cell line without IL-6 pretreatment). (B) RBE cells were treated with IL-6 and (CQ). Then western blotting was used to examine LC3 and p62 expression. (The Control b group was the RBE cell line without IL-6 and CQ pretreatment). (C-F) CCK-8 assays were employed to examine the cell viability in RBE and QBC939 cell lines treated with IL-6, CQ and 5-FU, cisplatin. *P
    Figure Legend Snippet: IL-6 promotes the chemoresistance of cholangiocarcinoma cells through activation of autophagy. (A) The expression of LC3 and p62 was detected by western blotting in the RBE cell line with treatment of IL-6. (The Control a group was the RBE cell line without IL-6 pretreatment). (B) RBE cells were treated with IL-6 and (CQ). Then western blotting was used to examine LC3 and p62 expression. (The Control b group was the RBE cell line without IL-6 and CQ pretreatment). (C-F) CCK-8 assays were employed to examine the cell viability in RBE and QBC939 cell lines treated with IL-6, CQ and 5-FU, cisplatin. *P

    Techniques Used: Activation Assay, Expressing, Western Blot, CCK-8 Assay

    Similar Products

  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 86
    MEDCHEMEXPRESS CO LTD 5 fluorouracil
    PRR14 inhibits CHEK2. CHEK2 mRNA expression ( a ), CHEK2 protein expression ( b ) and p-CHEK2 (T68) protein expression ( c ) in PRR14 genetically unaltered and altered (amplified and mutated) breast cancer cases in TCGA database are statistically analyzed by two-tailed Student’s t -test. CHEK2 protein expression is detected by immunostaining in xenografts in nude mice from established MCF7 cell lines and MDA-MB-231 cell lines ( d ), as well as human breast cancer ( e ). CHEK2 transcription in human breast cancer is also detected by qRT-PCR ( f ). The data are quantified and two-tailed Student’s t -test is employed to determine the significance of the difference. Established MCF7 and MDA-MB-231 ( g ) PRR14-overexpressing and control cell lines are treated with various of genotoxic chemicals including Bleo, Eto, <t>5-FU,</t> H2O2 and HU at indicated concentrations for indicated time. Key components of the ATM/CHEK2/P53 signaling pathway are detected by immunostaining. And CHEK2 protein expression ( h ) and mRNA expression ( i ) are detected by immunostaining and qRT-PCR, respectively. The data are analyzed by two-tailed Student’s t -test. Established MCF7 and MDA-MB-231 ( j ) PRR14-overexpressing and control cell lines are treated with Eto at indicated concentration for indicated time to induce p-CHEK2 (T68), which is detected by immunostaining and quantified and normalized by CHEK2 total protein ( k ). The data are analyzed by two-tailed Student’s t -test.
    5 Fluorouracil, supplied by MEDCHEMEXPRESS CO LTD, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/5 fluorouracil/product/MEDCHEMEXPRESS CO LTD
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    5 fluorouracil - by Bioz Stars, 2022-05
    86/100 stars
      Buy from Supplier

    94
    MedChemExpress 5 fluorouracil
    TTPAL promotes tumorigenicity and metastasis by regulating NNMT and PI3K/AKT signaling in vivo. A MGC803 cells stably expressing TTPAL expression promoted subcutaneous tumor growth as compared to control vector, both in terms of tumor volume over the entire assay period and tumor weight at the end point. B IHC staining confirmed TTPAL overexpression in MGC803 subcutaneous xenografts, which enhanced cell proliferation (by Ki-67 staining). IHC staining results also showed that TTPAL increased NNMT and p-AKT expression in xenografts. C Western blot analysis further confirmed that TTPAL expression in MGC803 xenografts increased the expression of NNMT and phospho-AKT. D Ectopic expression of TTPAL promoted experimental metastasis of MGC803 cells in vivo. Representative images of lungs and H E staining of lung tissues from nude mice injected with TTPAL or control vector-transfected MGC803 cells. Quantitative analysis showed that TTPAL expression significantly increased the number of metastatic lesions. E IHC staining results also showed that TTPAL enhanced cell proliferation (by Ki-67 staining) and increased NNMT and p-AKT expression in lung metastasis. F Knockdown of TTPAL significantly enhanced the inhibition of cell proliferation which mediated by <t>5-Fluorouracil</t> (5 μmol/l) and paclitaxel (5 nmol/l) in AGS and MKN74 cells as indicated by MTT assay. G . Schematic illustration of the molecular mechanism of TTPAL in PI3K/AKT signaling of GC.
    5 Fluorouracil, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/5 fluorouracil/product/MedChemExpress
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    5 fluorouracil - by Bioz Stars, 2022-05
    94/100 stars
      Buy from Supplier

    Image Search Results


    PRR14 inhibits CHEK2. CHEK2 mRNA expression ( a ), CHEK2 protein expression ( b ) and p-CHEK2 (T68) protein expression ( c ) in PRR14 genetically unaltered and altered (amplified and mutated) breast cancer cases in TCGA database are statistically analyzed by two-tailed Student’s t -test. CHEK2 protein expression is detected by immunostaining in xenografts in nude mice from established MCF7 cell lines and MDA-MB-231 cell lines ( d ), as well as human breast cancer ( e ). CHEK2 transcription in human breast cancer is also detected by qRT-PCR ( f ). The data are quantified and two-tailed Student’s t -test is employed to determine the significance of the difference. Established MCF7 and MDA-MB-231 ( g ) PRR14-overexpressing and control cell lines are treated with various of genotoxic chemicals including Bleo, Eto, 5-FU, H2O2 and HU at indicated concentrations for indicated time. Key components of the ATM/CHEK2/P53 signaling pathway are detected by immunostaining. And CHEK2 protein expression ( h ) and mRNA expression ( i ) are detected by immunostaining and qRT-PCR, respectively. The data are analyzed by two-tailed Student’s t -test. Established MCF7 and MDA-MB-231 ( j ) PRR14-overexpressing and control cell lines are treated with Eto at indicated concentration for indicated time to induce p-CHEK2 (T68), which is detected by immunostaining and quantified and normalized by CHEK2 total protein ( k ). The data are analyzed by two-tailed Student’s t -test.

    Journal: Cell Death & Disease

    Article Title: Oncogene PRR14 promotes breast cancer through activation of PI3K signal pathway and inhibition of CHEK2 pathway

    doi: 10.1038/s41419-020-2640-8

    Figure Lengend Snippet: PRR14 inhibits CHEK2. CHEK2 mRNA expression ( a ), CHEK2 protein expression ( b ) and p-CHEK2 (T68) protein expression ( c ) in PRR14 genetically unaltered and altered (amplified and mutated) breast cancer cases in TCGA database are statistically analyzed by two-tailed Student’s t -test. CHEK2 protein expression is detected by immunostaining in xenografts in nude mice from established MCF7 cell lines and MDA-MB-231 cell lines ( d ), as well as human breast cancer ( e ). CHEK2 transcription in human breast cancer is also detected by qRT-PCR ( f ). The data are quantified and two-tailed Student’s t -test is employed to determine the significance of the difference. Established MCF7 and MDA-MB-231 ( g ) PRR14-overexpressing and control cell lines are treated with various of genotoxic chemicals including Bleo, Eto, 5-FU, H2O2 and HU at indicated concentrations for indicated time. Key components of the ATM/CHEK2/P53 signaling pathway are detected by immunostaining. And CHEK2 protein expression ( h ) and mRNA expression ( i ) are detected by immunostaining and qRT-PCR, respectively. The data are analyzed by two-tailed Student’s t -test. Established MCF7 and MDA-MB-231 ( j ) PRR14-overexpressing and control cell lines are treated with Eto at indicated concentration for indicated time to induce p-CHEK2 (T68), which is detected by immunostaining and quantified and normalized by CHEK2 total protein ( k ). The data are analyzed by two-tailed Student’s t -test.

    Article Snippet: Chemicals including selective CHEK2 inhibitor BML-277 (BML, HY-13946, MCE) , and genotoxic chemicals, including bleomycin (Bleo, HY-17565, MCE), etoposide (Eto, HY-13629, MCE), 5-fluorouracil (5-FU, HY-90006, MCE), H2 O2 (88597, Millipore) and hydroxyurea (HU, HY-B0313, MCE), were used to treat cells.

    Techniques: Expressing, Amplification, Two Tailed Test, Immunostaining, Mouse Assay, Multiple Displacement Amplification, Quantitative RT-PCR, Concentration Assay

    TTPAL promotes tumorigenicity and metastasis by regulating NNMT and PI3K/AKT signaling in vivo. A MGC803 cells stably expressing TTPAL expression promoted subcutaneous tumor growth as compared to control vector, both in terms of tumor volume over the entire assay period and tumor weight at the end point. B IHC staining confirmed TTPAL overexpression in MGC803 subcutaneous xenografts, which enhanced cell proliferation (by Ki-67 staining). IHC staining results also showed that TTPAL increased NNMT and p-AKT expression in xenografts. C Western blot analysis further confirmed that TTPAL expression in MGC803 xenografts increased the expression of NNMT and phospho-AKT. D Ectopic expression of TTPAL promoted experimental metastasis of MGC803 cells in vivo. Representative images of lungs and H E staining of lung tissues from nude mice injected with TTPAL or control vector-transfected MGC803 cells. Quantitative analysis showed that TTPAL expression significantly increased the number of metastatic lesions. E IHC staining results also showed that TTPAL enhanced cell proliferation (by Ki-67 staining) and increased NNMT and p-AKT expression in lung metastasis. F Knockdown of TTPAL significantly enhanced the inhibition of cell proliferation which mediated by 5-Fluorouracil (5 μmol/l) and paclitaxel (5 nmol/l) in AGS and MKN74 cells as indicated by MTT assay. G . Schematic illustration of the molecular mechanism of TTPAL in PI3K/AKT signaling of GC.

    Journal: Oncogene

    Article Title: TTPAL promotes gastric tumorigenesis by directly targeting NNMT to activate PI3K/AKT signaling

    doi: 10.1038/s41388-021-01838-x

    Figure Lengend Snippet: TTPAL promotes tumorigenicity and metastasis by regulating NNMT and PI3K/AKT signaling in vivo. A MGC803 cells stably expressing TTPAL expression promoted subcutaneous tumor growth as compared to control vector, both in terms of tumor volume over the entire assay period and tumor weight at the end point. B IHC staining confirmed TTPAL overexpression in MGC803 subcutaneous xenografts, which enhanced cell proliferation (by Ki-67 staining). IHC staining results also showed that TTPAL increased NNMT and p-AKT expression in xenografts. C Western blot analysis further confirmed that TTPAL expression in MGC803 xenografts increased the expression of NNMT and phospho-AKT. D Ectopic expression of TTPAL promoted experimental metastasis of MGC803 cells in vivo. Representative images of lungs and H E staining of lung tissues from nude mice injected with TTPAL or control vector-transfected MGC803 cells. Quantitative analysis showed that TTPAL expression significantly increased the number of metastatic lesions. E IHC staining results also showed that TTPAL enhanced cell proliferation (by Ki-67 staining) and increased NNMT and p-AKT expression in lung metastasis. F Knockdown of TTPAL significantly enhanced the inhibition of cell proliferation which mediated by 5-Fluorouracil (5 μmol/l) and paclitaxel (5 nmol/l) in AGS and MKN74 cells as indicated by MTT assay. G . Schematic illustration of the molecular mechanism of TTPAL in PI3K/AKT signaling of GC.

    Article Snippet: PI3K inhibitor GDC-0941 was purchased from ApexBio (Shanghai, China) [ ], 5-Fluorouracil and paclitaxel were purchased from Med Chem Express (Shanghai, China).

    Techniques: In Vivo, Stable Transfection, Expressing, Plasmid Preparation, Immunohistochemistry, Staining, Over Expression, Western Blot, Mouse Assay, Injection, Transfection, Inhibition, MTT Assay

    Effects of 5-FU alone or combined with PP-26 on cell viability of HepG2 cells. The PP-26 concentration was 0.67 µmol/L and treatment time was 48 h (* P

    Journal: The Journal of International Medical Research

    Article Title: Paris polyphylla 26 triggers G2/M phase arrest and induces apoptosis in HepG2 cells via inhibition of the Akt signaling pathway

    doi: 10.1177/0300060519826823

    Figure Lengend Snippet: Effects of 5-FU alone or combined with PP-26 on cell viability of HepG2 cells. The PP-26 concentration was 0.67 µmol/L and treatment time was 48 h (* P

    Article Snippet: 5-fluorouracil (5-FU) was purchased from MedChemExpress (Monmouth Junction, NJ, USA).

    Techniques: Concentration Assay

    Phase contrast photomicrographs obtained by Crystal Violet staining of Huh7 cells treated with a control, b 5-FU, c TPGS-FA, d NC, and e TPGS-FA/NC at a concentration of 50 μg/mL for 24 h (Scale bar: 25 μm for original images)

    Journal: BMC Pharmacology & Toxicology

    Article Title: Folic acid modified TPGS as a novel nano-micelle for delivery of nitidine chloride to improve apoptosis induction in Huh7 human hepatocellular carcinoma

    doi: 10.1186/s40360-020-00461-y

    Figure Lengend Snippet: Phase contrast photomicrographs obtained by Crystal Violet staining of Huh7 cells treated with a control, b 5-FU, c TPGS-FA, d NC, and e TPGS-FA/NC at a concentration of 50 μg/mL for 24 h (Scale bar: 25 μm for original images)

    Article Snippet: 5-fluorouracil (5-Fu) was purchased from MedChemExpress (MCE, Monmouth Junction, NJ, USA).

    Techniques: Staining, Concentration Assay

    MTT assay of Huh7 cells treated with 5-FU, TPGS-FA, TPGS-FA/NC, and free NC at 7.5, 15,30, 60 and 120 μg/mL for 24 h. The data are expressed as mean ± S. D (n = 3)

    Journal: BMC Pharmacology & Toxicology

    Article Title: Folic acid modified TPGS as a novel nano-micelle for delivery of nitidine chloride to improve apoptosis induction in Huh7 human hepatocellular carcinoma

    doi: 10.1186/s40360-020-00461-y

    Figure Lengend Snippet: MTT assay of Huh7 cells treated with 5-FU, TPGS-FA, TPGS-FA/NC, and free NC at 7.5, 15,30, 60 and 120 μg/mL for 24 h. The data are expressed as mean ± S. D (n = 3)

    Article Snippet: 5-fluorouracil (5-Fu) was purchased from MedChemExpress (MCE, Monmouth Junction, NJ, USA).

    Techniques: MTT Assay

    Flow cytometry plots of control Huh7 cells and the groups treated with 5-FU, TPGS-FA, NC, and TPGS-FA/NC at a concentration of 50 μg/mL for 24 h. In vitro apoptosis was observed by propidium iodide (PI)/Annexin V-FITC dual staining and fluorescence-activated cell sorting (FACS) analysis (Q2 = Annexin V-FITC and PI positive, indicating cells in late apoptosis or already dead; Q3 = PI negative Annexin V-FITC positive, indicating early apoptotic cells). Source data are provided as a Source Data file

    Journal: BMC Pharmacology & Toxicology

    Article Title: Folic acid modified TPGS as a novel nano-micelle for delivery of nitidine chloride to improve apoptosis induction in Huh7 human hepatocellular carcinoma

    doi: 10.1186/s40360-020-00461-y

    Figure Lengend Snippet: Flow cytometry plots of control Huh7 cells and the groups treated with 5-FU, TPGS-FA, NC, and TPGS-FA/NC at a concentration of 50 μg/mL for 24 h. In vitro apoptosis was observed by propidium iodide (PI)/Annexin V-FITC dual staining and fluorescence-activated cell sorting (FACS) analysis (Q2 = Annexin V-FITC and PI positive, indicating cells in late apoptosis or already dead; Q3 = PI negative Annexin V-FITC positive, indicating early apoptotic cells). Source data are provided as a Source Data file

    Article Snippet: 5-fluorouracil (5-Fu) was purchased from MedChemExpress (MCE, Monmouth Junction, NJ, USA).

    Techniques: Flow Cytometry, Concentration Assay, In Vitro, Staining, Fluorescence, FACS

    CLSM images of Huh7 cells treated with a control, b 5-FU, c TPGS-FA, d NC, and e TPGS-FA/NC at a concentration of 50 μg/mL for 24 h, respectively. Scale bar: 75 μm for original images

    Journal: BMC Pharmacology & Toxicology

    Article Title: Folic acid modified TPGS as a novel nano-micelle for delivery of nitidine chloride to improve apoptosis induction in Huh7 human hepatocellular carcinoma

    doi: 10.1186/s40360-020-00461-y

    Figure Lengend Snippet: CLSM images of Huh7 cells treated with a control, b 5-FU, c TPGS-FA, d NC, and e TPGS-FA/NC at a concentration of 50 μg/mL for 24 h, respectively. Scale bar: 75 μm for original images

    Article Snippet: 5-fluorouracil (5-Fu) was purchased from MedChemExpress (MCE, Monmouth Junction, NJ, USA).

    Techniques: Confocal Laser Scanning Microscopy, Concentration Assay