4e2rcat  (MedChemExpress)


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    MedChemExpress 4e2rcat
    SARS-CoV-2 untranslated regions support eIF4F-dependent translation. (A) Schematic of luciferase reporter construct. (B) Inhibition of reporter translation in a HeLa cell-free translation assay by the small-molecule inhibitor of eIF4E–eIF4G interaction, <t>4E2RCat.</t> Luciferase translation is normalized to a control reaction without inhibitor. (C) Inhibition of reporter translation by the small-molecule eIF4A inhibitor, RocA (rocaglamide). Error bars in (B) and (C) reflect the standard deviation from three independent luciferase reactions, with the luminescence from each independent reaction measured in triplicate.
    4e2rcat, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/4e2rcat/product/MedChemExpress
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    4e2rcat - by Bioz Stars, 2022-05
    93/100 stars

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    1) Product Images from "Single-Molecule Dynamics of SARS-CoV-2 5’ Cap Recognition by Human eIF4F"

    Article Title: Single-Molecule Dynamics of SARS-CoV-2 5’ Cap Recognition by Human eIF4F

    Journal: bioRxiv

    doi: 10.1101/2021.05.26.445185

    SARS-CoV-2 untranslated regions support eIF4F-dependent translation. (A) Schematic of luciferase reporter construct. (B) Inhibition of reporter translation in a HeLa cell-free translation assay by the small-molecule inhibitor of eIF4E–eIF4G interaction, 4E2RCat. Luciferase translation is normalized to a control reaction without inhibitor. (C) Inhibition of reporter translation by the small-molecule eIF4A inhibitor, RocA (rocaglamide). Error bars in (B) and (C) reflect the standard deviation from three independent luciferase reactions, with the luminescence from each independent reaction measured in triplicate.
    Figure Legend Snippet: SARS-CoV-2 untranslated regions support eIF4F-dependent translation. (A) Schematic of luciferase reporter construct. (B) Inhibition of reporter translation in a HeLa cell-free translation assay by the small-molecule inhibitor of eIF4E–eIF4G interaction, 4E2RCat. Luciferase translation is normalized to a control reaction without inhibitor. (C) Inhibition of reporter translation by the small-molecule eIF4A inhibitor, RocA (rocaglamide). Error bars in (B) and (C) reflect the standard deviation from three independent luciferase reactions, with the luminescence from each independent reaction measured in triplicate.

    Techniques Used: Luciferase, Construct, Inhibition, Standard Deviation

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    MedChemExpress 4e2rcat
    SARS-CoV-2 untranslated regions support eIF4F-dependent translation. (A) Schematic of luciferase reporter construct. (B) Inhibition of reporter translation in a HeLa cell-free translation assay by the small-molecule inhibitor of eIF4E–eIF4G interaction, <t>4E2RCat.</t> Luciferase translation is normalized to a control reaction without inhibitor. (C) Inhibition of reporter translation by the small-molecule eIF4A inhibitor, RocA (rocaglamide). Error bars in (B) and (C) reflect the standard deviation from three independent luciferase reactions, with the luminescence from each independent reaction measured in triplicate.
    4e2rcat, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/4e2rcat/product/MedChemExpress
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    4e2rcat - by Bioz Stars, 2022-05
    93/100 stars
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    SARS-CoV-2 untranslated regions support eIF4F-dependent translation. (A) Schematic of luciferase reporter construct. (B) Inhibition of reporter translation in a HeLa cell-free translation assay by the small-molecule inhibitor of eIF4E–eIF4G interaction, 4E2RCat. Luciferase translation is normalized to a control reaction without inhibitor. (C) Inhibition of reporter translation by the small-molecule eIF4A inhibitor, RocA (rocaglamide). Error bars in (B) and (C) reflect the standard deviation from three independent luciferase reactions, with the luminescence from each independent reaction measured in triplicate.

    Journal: bioRxiv

    Article Title: Single-Molecule Dynamics of SARS-CoV-2 5’ Cap Recognition by Human eIF4F

    doi: 10.1101/2021.05.26.445185

    Figure Lengend Snippet: SARS-CoV-2 untranslated regions support eIF4F-dependent translation. (A) Schematic of luciferase reporter construct. (B) Inhibition of reporter translation in a HeLa cell-free translation assay by the small-molecule inhibitor of eIF4E–eIF4G interaction, 4E2RCat. Luciferase translation is normalized to a control reaction without inhibitor. (C) Inhibition of reporter translation by the small-molecule eIF4A inhibitor, RocA (rocaglamide). Error bars in (B) and (C) reflect the standard deviation from three independent luciferase reactions, with the luminescence from each independent reaction measured in triplicate.

    Article Snippet: Luciferase assays 100 nM CoV firefly luciferase fusion RNA was added to 12 µL of HeLa lysate reaction mix from an in vitro protein expression kit (Thermo Scientific, 88882), along with 0.5 µL of rocaglamide/RocA solution (Med Chem Express, Product No. HY-19356) or 4E2RCat solution (Med Chem Express, Product No. HY-100733) in varying concentrations as indicated in the Results and Discussion.

    Techniques: Luciferase, Construct, Inhibition, Standard Deviation