a204  (ATCC)


Bioz Verified Symbol ATCC is a verified supplier
Bioz Manufacturer Symbol ATCC manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 99

    Structured Review

    ATCC a204
    IC 50 values for ATRA, 4-HPR, and derivatives on various tumor cell lines
    A204, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/a204/product/ATCC
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    a204 - by Bioz Stars, 2024-05
    99/100 stars

    Images

    1) Product Images from "In vitro activities of novel 4-HPR derivatives on a panel of rhabdoid and other tumor cell lines"

    Article Title: In vitro activities of novel 4-HPR derivatives on a panel of rhabdoid and other tumor cell lines

    Journal: Cancer Cell International

    doi: 10.1186/1475-2867-11-34

    IC 50 values for ATRA, 4-HPR, and derivatives on various tumor cell lines
    Figure Legend Snippet: IC 50 values for ATRA, 4-HPR, and derivatives on various tumor cell lines

    Techniques Used:

    Inhibition of RT cell growth by 4-HPR and its derivatives and peptidomimetics : Survival curves of MON (A), G401 (B) and A204 (C) cells treated for three days with increasing concentrations of ATRA, 4-HPR, the most active halogen substituted derivative of 4-HPR (5h and 5j) and peptidomimetic compounds (11a, 11c and 11d).
    Figure Legend Snippet: Inhibition of RT cell growth by 4-HPR and its derivatives and peptidomimetics : Survival curves of MON (A), G401 (B) and A204 (C) cells treated for three days with increasing concentrations of ATRA, 4-HPR, the most active halogen substituted derivative of 4-HPR (5h and 5j) and peptidomimetic compounds (11a, 11c and 11d).

    Techniques Used: Inhibition

    Induction of cell cycle arrest by 4-HPR and its derivatives and peptidomimetics : A-C. Cell cycle profile of MON (A), G401 (B), and A204 (C) cells treated for three days with 5 μM or 10 μM concentrations of ATRA, 4-HPR, and the iodo-conjugated 4-HPR derivative (5j) and peptidomimetic (11d).
    Figure Legend Snippet: Induction of cell cycle arrest by 4-HPR and its derivatives and peptidomimetics : A-C. Cell cycle profile of MON (A), G401 (B), and A204 (C) cells treated for three days with 5 μM or 10 μM concentrations of ATRA, 4-HPR, and the iodo-conjugated 4-HPR derivative (5j) and peptidomimetic (11d).

    Techniques Used:

    Induction of cell death by 4-HPR and its derivatives and peptidomimetics : A-C. Percentage of MON (A), G401 (B), and A204 (C) cells at sub- G 1 when treated for three days with 5 μM or 10 μM concentrations of ATRA, 4-HPR, and the iodo-conjugated 4-HPR derivative (5j) and peptidomimetic (11d).
    Figure Legend Snippet: Induction of cell death by 4-HPR and its derivatives and peptidomimetics : A-C. Percentage of MON (A), G401 (B), and A204 (C) cells at sub- G 1 when treated for three days with 5 μM or 10 μM concentrations of ATRA, 4-HPR, and the iodo-conjugated 4-HPR derivative (5j) and peptidomimetic (11d).

    Techniques Used:

    htb 82 rrid cvcl 1058 human  (ATCC)


    Bioz Verified Symbol ATCC is a verified supplier
    Bioz Manufacturer Symbol ATCC manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 86

    Structured Review

    ATCC htb 82 rrid cvcl 1058 human
    Htb 82 Rrid Cvcl 1058 Human, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/htb 82 rrid cvcl 1058 human/product/ATCC
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    htb 82 rrid cvcl 1058 human - by Bioz Stars, 2024-05
    86/100 stars

    Images

    a 172 crl 1620 glioblastoma 1 067 a 204 htb 82 rhabdomyosarcoma 1 360 a375 crl  (ATCC)


    Bioz Verified Symbol ATCC is a verified supplier
    Bioz Manufacturer Symbol ATCC manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 86

    Structured Review

    ATCC a 172 crl 1620 glioblastoma 1 067 a 204 htb 82 rhabdomyosarcoma 1 360 a375 crl
    A 172 Crl 1620 Glioblastoma 1 067 A 204 Htb 82 Rhabdomyosarcoma 1 360 A375 Crl, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/a 172 crl 1620 glioblastoma 1 067 a 204 htb 82 rhabdomyosarcoma 1 360 a375 crl/product/ATCC
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    a 172 crl 1620 glioblastoma 1 067 a 204 htb 82 rhabdomyosarcoma 1 360 a375 crl - by Bioz Stars, 2024-05
    86/100 stars

    Images

    a204  (ATCC)


    Bioz Verified Symbol ATCC is a verified supplier
    Bioz Manufacturer Symbol ATCC manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 99

    Structured Review

    ATCC a204
    IC 50 values for ATRA, 4-HPR, and derivatives on various tumor cell lines
    A204, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/a204/product/ATCC
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    a204 - by Bioz Stars, 2024-05
    99/100 stars

    Images

    1) Product Images from "In vitro activities of novel 4-HPR derivatives on a panel of rhabdoid and other tumor cell lines"

    Article Title: In vitro activities of novel 4-HPR derivatives on a panel of rhabdoid and other tumor cell lines

    Journal: Cancer Cell International

    doi: 10.1186/1475-2867-11-34

    IC 50 values for ATRA, 4-HPR, and derivatives on various tumor cell lines
    Figure Legend Snippet: IC 50 values for ATRA, 4-HPR, and derivatives on various tumor cell lines

    Techniques Used:

    Inhibition of RT cell growth by 4-HPR and its derivatives and peptidomimetics : Survival curves of MON (A), G401 (B) and A204 (C) cells treated for three days with increasing concentrations of ATRA, 4-HPR, the most active halogen substituted derivative of 4-HPR (5h and 5j) and peptidomimetic compounds (11a, 11c and 11d).
    Figure Legend Snippet: Inhibition of RT cell growth by 4-HPR and its derivatives and peptidomimetics : Survival curves of MON (A), G401 (B) and A204 (C) cells treated for three days with increasing concentrations of ATRA, 4-HPR, the most active halogen substituted derivative of 4-HPR (5h and 5j) and peptidomimetic compounds (11a, 11c and 11d).

    Techniques Used: Inhibition

    Induction of cell cycle arrest by 4-HPR and its derivatives and peptidomimetics : A-C. Cell cycle profile of MON (A), G401 (B), and A204 (C) cells treated for three days with 5 μM or 10 μM concentrations of ATRA, 4-HPR, and the iodo-conjugated 4-HPR derivative (5j) and peptidomimetic (11d).
    Figure Legend Snippet: Induction of cell cycle arrest by 4-HPR and its derivatives and peptidomimetics : A-C. Cell cycle profile of MON (A), G401 (B), and A204 (C) cells treated for three days with 5 μM or 10 μM concentrations of ATRA, 4-HPR, and the iodo-conjugated 4-HPR derivative (5j) and peptidomimetic (11d).

    Techniques Used:

    Induction of cell death by 4-HPR and its derivatives and peptidomimetics : A-C. Percentage of MON (A), G401 (B), and A204 (C) cells at sub- G 1 when treated for three days with 5 μM or 10 μM concentrations of ATRA, 4-HPR, and the iodo-conjugated 4-HPR derivative (5j) and peptidomimetic (11d).
    Figure Legend Snippet: Induction of cell death by 4-HPR and its derivatives and peptidomimetics : A-C. Percentage of MON (A), G401 (B), and A204 (C) cells at sub- G 1 when treated for three days with 5 μM or 10 μM concentrations of ATRA, 4-HPR, and the iodo-conjugated 4-HPR derivative (5j) and peptidomimetic (11d).

    Techniques Used:

    htb82 erms cell lines  (ATCC)


    Bioz Verified Symbol ATCC is a verified supplier
    Bioz Manufacturer Symbol ATCC manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 99

    Structured Review

    ATCC htb82 erms cell lines
    Formation of holoclones and spheres in RMS cell lines. (a) Representative images of holoclones, meroclones, and paraclones in CW9019 and <t>HTB82</t> cell lines. (b) Percentages of clones classified as holoclones, meroclones, or paraclones in CW9019, HTB82, RD, and RH30 cell lines. (c) Representative images of CW9019 and HTB82 spheres. (d) Number of spheres formed per 1000 cells seeded in neurosphere media. Data were expressed as mean ± SEM of three independent experiments.
    Htb82 Erms Cell Lines, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/htb82 erms cell lines/product/ATCC
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    htb82 erms cell lines - by Bioz Stars, 2024-05
    99/100 stars

    Images

    1) Product Images from "Hedgehog Pathway Inhibition Hampers Sphere and Holoclone Formation in Rhabdomyosarcoma"

    Article Title: Hedgehog Pathway Inhibition Hampers Sphere and Holoclone Formation in Rhabdomyosarcoma

    Journal: Stem Cells International

    doi: 10.1155/2017/7507380

    Formation of holoclones and spheres in RMS cell lines. (a) Representative images of holoclones, meroclones, and paraclones in CW9019 and HTB82 cell lines. (b) Percentages of clones classified as holoclones, meroclones, or paraclones in CW9019, HTB82, RD, and RH30 cell lines. (c) Representative images of CW9019 and HTB82 spheres. (d) Number of spheres formed per 1000 cells seeded in neurosphere media. Data were expressed as mean ± SEM of three independent experiments.
    Figure Legend Snippet: Formation of holoclones and spheres in RMS cell lines. (a) Representative images of holoclones, meroclones, and paraclones in CW9019 and HTB82 cell lines. (b) Percentages of clones classified as holoclones, meroclones, or paraclones in CW9019, HTB82, RD, and RH30 cell lines. (c) Representative images of CW9019 and HTB82 spheres. (d) Number of spheres formed per 1000 cells seeded in neurosphere media. Data were expressed as mean ± SEM of three independent experiments.

    Techniques Used: Clone Assay

    Self-renewal capacity of cells contained in RMS holoclones and spheres. ((a) and (b)) Initial percentage of the three types of clones generated in CW9019 and HTB82 cell lines, respectively. Representative images and quantification of secondary clones formed from holoclones (c and d), meroclones (e and f), and paraclones (g and h) in CW9019 and HTB82 cell lines, respectively. Enrichment in the secondary sphere fraction obtained in CW9019 (i) and HTB82 (j) cell lines. All experiments were tested in triplicate. Significance: ∗ p < 0.05; ∗∗ p < 0.01.
    Figure Legend Snippet: Self-renewal capacity of cells contained in RMS holoclones and spheres. ((a) and (b)) Initial percentage of the three types of clones generated in CW9019 and HTB82 cell lines, respectively. Representative images and quantification of secondary clones formed from holoclones (c and d), meroclones (e and f), and paraclones (g and h) in CW9019 and HTB82 cell lines, respectively. Enrichment in the secondary sphere fraction obtained in CW9019 (i) and HTB82 (j) cell lines. All experiments were tested in triplicate. Significance: ∗ p < 0.05; ∗∗ p < 0.01.

    Techniques Used: Clone Assay, Generated

    RMS stem-like cells showed GLI1 upregulation. Analysis of SHH, IHH, DHH, and GLI1 mRNA levels in holoclones (a, b, c, and d) and spheres (e, f, g, and h) of CW9010 and HTB82 cell lines. Values were referred to expression levels of meroclones and adherent cells (black bars), respectively. Data were tested in triplicate and expressed as mean ± SEM. Significance: ∗ p < 0.05; ∗∗ p < 0.01; ∗∗∗ p < 0.001.
    Figure Legend Snippet: RMS stem-like cells showed GLI1 upregulation. Analysis of SHH, IHH, DHH, and GLI1 mRNA levels in holoclones (a, b, c, and d) and spheres (e, f, g, and h) of CW9010 and HTB82 cell lines. Values were referred to expression levels of meroclones and adherent cells (black bars), respectively. Data were tested in triplicate and expressed as mean ± SEM. Significance: ∗ p < 0.05; ∗∗ p < 0.01; ∗∗∗ p < 0.001.

    Techniques Used: Expressing

    Effects of HH ligands and GLI1 depletion on RMS holoclone and sphere formation. ((a) and (b)) Plots representing the percentage of each colony type formed by SHH , IHH , DHH , and GLI1 shRNA-expressing CW9010 and HTB82 cells, respectively. ((c) and (d)) Plots representing the relative diameter of spheres formed by shRNA-expressing cells. Values were referred to control cells (transfected with pGIPZ empty vector) and expressed as mean ± SEM of three independent experiments. From (e) to (h) real-time PCRs to assess the downregulation of all shRNA targets: SHH, IHH, DHH , and GLI1 , respectively. Significance: ∗ p < 0.05; ∗∗ p < 0.01; ∗∗∗ p < 0.001.
    Figure Legend Snippet: Effects of HH ligands and GLI1 depletion on RMS holoclone and sphere formation. ((a) and (b)) Plots representing the percentage of each colony type formed by SHH , IHH , DHH , and GLI1 shRNA-expressing CW9010 and HTB82 cells, respectively. ((c) and (d)) Plots representing the relative diameter of spheres formed by shRNA-expressing cells. Values were referred to control cells (transfected with pGIPZ empty vector) and expressed as mean ± SEM of three independent experiments. From (e) to (h) real-time PCRs to assess the downregulation of all shRNA targets: SHH, IHH, DHH , and GLI1 , respectively. Significance: ∗ p < 0.05; ∗∗ p < 0.01; ∗∗∗ p < 0.001.

    Techniques Used: shRNA, Expressing, Transfection, Plasmid Preparation

    HH pathway inhibitors hindered holoclone and sphere formation. CW9019 and HTB82 cells were pretreated with Sonidegib ( SMO inhibitor) and MEDI-5304 (HH ligand blocking antibody) 48 h prior to holoclone- and sphere-formation assays. ((a) and (b)) Plots representing the percentage of each colony type formed after HH pathway inhibitor treatment. Percentages of number (c and d) and diameter (e and f) of spheres formed after HH pathway inhibitor treatment. Values were referred to control cells (treated with vehicle) and expressed as mean ± SEM of three independent experiments. Significance: ∗ p < 0.05; ∗∗ p < 0.01; referred to holoclones of control cells and # p < 0.05; ## p < 0.01 referred to paraclones of control cells.
    Figure Legend Snippet: HH pathway inhibitors hindered holoclone and sphere formation. CW9019 and HTB82 cells were pretreated with Sonidegib ( SMO inhibitor) and MEDI-5304 (HH ligand blocking antibody) 48 h prior to holoclone- and sphere-formation assays. ((a) and (b)) Plots representing the percentage of each colony type formed after HH pathway inhibitor treatment. Percentages of number (c and d) and diameter (e and f) of spheres formed after HH pathway inhibitor treatment. Values were referred to control cells (treated with vehicle) and expressed as mean ± SEM of three independent experiments. Significance: ∗ p < 0.05; ∗∗ p < 0.01; referred to holoclones of control cells and # p < 0.05; ## p < 0.01 referred to paraclones of control cells.

    Techniques Used: Blocking Assay

    Holoclones showed induction of stem cell markers. mRNA levels of OCT4, NANOG, PAX7, and KITLG were evaluated in CW9019 and HTB82 cell lines. The expression levels of these markers were evaluated by conventional RT-PCR in total cell lines (line) and holoclones of first (holo1) and second (holo2) passages.
    Figure Legend Snippet: Holoclones showed induction of stem cell markers. mRNA levels of OCT4, NANOG, PAX7, and KITLG were evaluated in CW9019 and HTB82 cell lines. The expression levels of these markers were evaluated by conventional RT-PCR in total cell lines (line) and holoclones of first (holo1) and second (holo2) passages.

    Techniques Used: Expressing, Reverse Transcription Polymerase Chain Reaction

    j 82 htb 1  (ATCC)


    Bioz Verified Symbol ATCC is a verified supplier
    Bioz Manufacturer Symbol ATCC manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 95

    Structured Review

    ATCC j 82 htb 1
    J 82 Htb 1, supplied by ATCC, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/j 82 htb 1/product/ATCC
    Average 95 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    j 82 htb 1 - by Bioz Stars, 2024-05
    95/100 stars

    Images

    a204 cells  (ATCC)


    Bioz Verified Symbol ATCC is a verified supplier
    Bioz Manufacturer Symbol ATCC manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 99

    Structured Review

    ATCC a204 cells
    Inhibition of rhabdoid tumor growth by flavopiridol and 4OH-Tam . A-C . Survival curves of MON (A), G401 (B), and <t>A204</t> (C) cells treated with flavopiridol in the presence and absence of 4OH-Tam for two days. D . Table of IC50 values as approximated from A-C. Flavo = flavopiridol; 4OHT = 4OH-Tam.
    A204 Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/a204 cells/product/ATCC
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    a204 cells - by Bioz Stars, 2024-05
    99/100 stars

    Images

    1) Product Images from "Potent inhibition of rhabdoid tumor cells by combination of flavopiridol and 4OH-tamoxifen"

    Article Title: Potent inhibition of rhabdoid tumor cells by combination of flavopiridol and 4OH-tamoxifen

    Journal: BMC Cancer

    doi: 10.1186/1471-2407-10-634

    Inhibition of rhabdoid tumor growth by flavopiridol and 4OH-Tam . A-C . Survival curves of MON (A), G401 (B), and A204 (C) cells treated with flavopiridol in the presence and absence of 4OH-Tam for two days. D . Table of IC50 values as approximated from A-C. Flavo = flavopiridol; 4OHT = 4OH-Tam.
    Figure Legend Snippet: Inhibition of rhabdoid tumor growth by flavopiridol and 4OH-Tam . A-C . Survival curves of MON (A), G401 (B), and A204 (C) cells treated with flavopiridol in the presence and absence of 4OH-Tam for two days. D . Table of IC50 values as approximated from A-C. Flavo = flavopiridol; 4OHT = 4OH-Tam.

    Techniques Used: Inhibition

    a 204  (ATCC)


    Bioz Verified Symbol ATCC is a verified supplier
    Bioz Manufacturer Symbol ATCC manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 99

    Structured Review

    ATCC a 204
    MYOD1+ NOG+ cells predominant survival in RD and <t>A-204</t> cell cultures after treatment with either Vincristine or Doxorubicin. (a) Plots show examples of flow analysis of RD and A-204 cells treated with Vincristine and Doxorubicin. In grey is highlighted the quadrant representing MYOD1+ NOG+ cells. (b) Percentage of live MYOD1+ NOG+ phenotype cells in RD and A-204 cell cultures after 2 days of treatment either with 1 and 10 nM of Vincristine (top panel) or with 0.1 and 1 μ M of Doxorubicin (bottom panel). (c) Absolute numbers of total live cells in RD and A-204 cultures treated with 10 nM of Vincristine or 1 μ M of Doxorubicin for 2 days. Data are presented as a ratio of absolute numbers of cells in treated cultures to absolute numbers of cells in nontreated cultures (fold change). Data are shown as mean ± standard deviation ( N = 11). ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001, ANOVA with Tukey's multiple comparison test.
    A 204, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/a 204/product/ATCC
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    a 204 - by Bioz Stars, 2024-05
    99/100 stars

    Images

    1) Product Images from "Induction of Myogenic Differentiation Improves Chemosensitivity of Chemoresistant Cells in Soft-Tissue Sarcoma Cell Lines"

    Article Title: Induction of Myogenic Differentiation Improves Chemosensitivity of Chemoresistant Cells in Soft-Tissue Sarcoma Cell Lines

    Journal: Sarcoma

    doi: 10.1155/2020/8647981

    MYOD1+ NOG+ cells predominant survival in RD and A-204 cell cultures after treatment with either Vincristine or Doxorubicin. (a) Plots show examples of flow analysis of RD and A-204 cells treated with Vincristine and Doxorubicin. In grey is highlighted the quadrant representing MYOD1+ NOG+ cells. (b) Percentage of live MYOD1+ NOG+ phenotype cells in RD and A-204 cell cultures after 2 days of treatment either with 1 and 10 nM of Vincristine (top panel) or with 0.1 and 1 μ M of Doxorubicin (bottom panel). (c) Absolute numbers of total live cells in RD and A-204 cultures treated with 10 nM of Vincristine or 1 μ M of Doxorubicin for 2 days. Data are presented as a ratio of absolute numbers of cells in treated cultures to absolute numbers of cells in nontreated cultures (fold change). Data are shown as mean ± standard deviation ( N = 11). ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001, ANOVA with Tukey's multiple comparison test.
    Figure Legend Snippet: MYOD1+ NOG+ cells predominant survival in RD and A-204 cell cultures after treatment with either Vincristine or Doxorubicin. (a) Plots show examples of flow analysis of RD and A-204 cells treated with Vincristine and Doxorubicin. In grey is highlighted the quadrant representing MYOD1+ NOG+ cells. (b) Percentage of live MYOD1+ NOG+ phenotype cells in RD and A-204 cell cultures after 2 days of treatment either with 1 and 10 nM of Vincristine (top panel) or with 0.1 and 1 μ M of Doxorubicin (bottom panel). (c) Absolute numbers of total live cells in RD and A-204 cultures treated with 10 nM of Vincristine or 1 μ M of Doxorubicin for 2 days. Data are presented as a ratio of absolute numbers of cells in treated cultures to absolute numbers of cells in nontreated cultures (fold change). Data are shown as mean ± standard deviation ( N = 11). ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001, ANOVA with Tukey's multiple comparison test.

    Techniques Used: Standard Deviation

    MYOD1+ NOG+ cells express the highest levels of antiapoptotic BCL2 protein among other cells in RD and A-204 cultures. Median fluorescence intensity (MFI) of BCL2 protein in MYOD1/NOG cell subpopulations of RD (left plot) and A-204 (right plot) cell lines. Data are presented as a ratio of BCL2 MFI of MYOD1+ NOG+ cells and MYOD1− NOG+ cells to BCL2 MFI of MYOD1− NOG− cells (fold change), (mean ± standard deviation, N = 9). ∗ p < 0.05, ∗∗ p < 0.01, ANOVA with Tukey's multiple comparison test).
    Figure Legend Snippet: MYOD1+ NOG+ cells express the highest levels of antiapoptotic BCL2 protein among other cells in RD and A-204 cultures. Median fluorescence intensity (MFI) of BCL2 protein in MYOD1/NOG cell subpopulations of RD (left plot) and A-204 (right plot) cell lines. Data are presented as a ratio of BCL2 MFI of MYOD1+ NOG+ cells and MYOD1− NOG+ cells to BCL2 MFI of MYOD1− NOG− cells (fold change), (mean ± standard deviation, N = 9). ∗ p < 0.05, ∗∗ p < 0.01, ANOVA with Tukey's multiple comparison test).

    Techniques Used: Fluorescence, Standard Deviation

    Differentiation therapy increases the effectiveness of chemotherapy. Absolute numbers of total live cells RD (a) and A-204 (b) in cultures treated for 6 days with TPA/GSK126, 10 nM of Vincristine, or combination of Vincristine and TPA/GSK126. Data are presented as a ratio of absolute numbers of cells in treated cultures to absolute numbers of cells in nontreated cultures (fold change) (mean ± standard deviation, N = 4). ∗ p < 0.05, ∗∗∗ p < 0.001, ANOVA with Tukey's multiple comparison test for the analysis between the groups.
    Figure Legend Snippet: Differentiation therapy increases the effectiveness of chemotherapy. Absolute numbers of total live cells RD (a) and A-204 (b) in cultures treated for 6 days with TPA/GSK126, 10 nM of Vincristine, or combination of Vincristine and TPA/GSK126. Data are presented as a ratio of absolute numbers of cells in treated cultures to absolute numbers of cells in nontreated cultures (fold change) (mean ± standard deviation, N = 4). ∗ p < 0.05, ∗∗∗ p < 0.001, ANOVA with Tukey's multiple comparison test for the analysis between the groups.

    Techniques Used: Standard Deviation

    a204  (ATCC)


    Bioz Verified Symbol ATCC is a verified supplier
    Bioz Manufacturer Symbol ATCC manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 99

    Structured Review

    ATCC a204
    Synergistic effect of BYL-719 + CAL-101 in reducing rhabdomyosarcoma cell viability. ( A ) MTT assay in RD, <t>A204</t> and SJCRH30 cells treated with BYL-719, CAL-101, BYL-719 + CAL-101, and AZD8835 for 72 h. Cells were seeded in 96-well plates and treated in triplicate with increasing concentrations of inhibitors. After 72 h, a cell proliferation assay was carried-out and the absorbance intensity measured in a microplate reader. Data are representative of three independent experiments. ( B ) Isobologram for combination index (CI) calculations from combined treatment with BYL-719 and CAL-101 for 72 h. The lines link the corresponding concentrations of the two drugs which singularly determine the affected fraction (ED90, ED75, ED50). For each fraction, the corresponding values of CI are reported, with the relative position in the graph (x, +, ⨀).
    A204, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/a204/product/ATCC
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    a204 - by Bioz Stars, 2024-05
    99/100 stars

    Images

    1) Product Images from "Combined Treatment with PI3K Inhibitors BYL-719 and CAL-101 Is a Promising Antiproliferative Strategy in Human Rhabdomyosarcoma Cells"

    Article Title: Combined Treatment with PI3K Inhibitors BYL-719 and CAL-101 Is a Promising Antiproliferative Strategy in Human Rhabdomyosarcoma Cells

    Journal: Molecules

    doi: 10.3390/molecules27092742

    Synergistic effect of BYL-719 + CAL-101 in reducing rhabdomyosarcoma cell viability. ( A ) MTT assay in RD, A204 and SJCRH30 cells treated with BYL-719, CAL-101, BYL-719 + CAL-101, and AZD8835 for 72 h. Cells were seeded in 96-well plates and treated in triplicate with increasing concentrations of inhibitors. After 72 h, a cell proliferation assay was carried-out and the absorbance intensity measured in a microplate reader. Data are representative of three independent experiments. ( B ) Isobologram for combination index (CI) calculations from combined treatment with BYL-719 and CAL-101 for 72 h. The lines link the corresponding concentrations of the two drugs which singularly determine the affected fraction (ED90, ED75, ED50). For each fraction, the corresponding values of CI are reported, with the relative position in the graph (x, +, ⨀).
    Figure Legend Snippet: Synergistic effect of BYL-719 + CAL-101 in reducing rhabdomyosarcoma cell viability. ( A ) MTT assay in RD, A204 and SJCRH30 cells treated with BYL-719, CAL-101, BYL-719 + CAL-101, and AZD8835 for 72 h. Cells were seeded in 96-well plates and treated in triplicate with increasing concentrations of inhibitors. After 72 h, a cell proliferation assay was carried-out and the absorbance intensity measured in a microplate reader. Data are representative of three independent experiments. ( B ) Isobologram for combination index (CI) calculations from combined treatment with BYL-719 and CAL-101 for 72 h. The lines link the corresponding concentrations of the two drugs which singularly determine the affected fraction (ED90, ED75, ED50). For each fraction, the corresponding values of CI are reported, with the relative position in the graph (x, +, ⨀).

    Techniques Used: MTT Assay, Proliferation Assay

    Combination of BYL-719 + CAL-101 arrests rhabdomyosarcoma cell cycle and induces caspase-dependent apoptosis. ( A ) RD, SJCRH30, and A204 cells were seeded in a 6-well plate at a concentration of 2 × 10 5 cells/well. Cells were treated with 5 μM of either Alpelisib (BYL-719), Idelalisib (CAL-101), AZD8835 or BYL-719 + CAL-101 (2.5 μM + 2.5 μM), or left untreated. After 24, 48, and 72 h, cells were collected and counted. Cell viability was assessed by trypan blue exclusion. Histograms show the percentage of viable cells and are representative of three independent experiments. *** p < 0.001. ( B ) Cell cycle analysis of RD, A204 and SJCRH30 cells treated with 5 μM of either BYL-719, CAL-101, AZD8835 or BYL-719 + CAL-101 (2.5 μM + 2.5 μM), or left untreated for 72 h. RD, SJCRH30, and A204 were seeded at the concentration of 1 × 10 6 cells/well; after 24 h, cells were treated as described above, stained with propidium iodide (PI) and subjected to FACS analysis. Histograms show the percent distribution of cells in each of the cell cycle phases and are representative of three independent experiments. ( C ) RD, SJCRH30, and A204 were seeded at the concentration of 1 × 10 6 cells in 100 mm plates. After an overnight incubation, cells were treated with 5 μM of either BYL-719, CAL101, AZD8835, or BYL-719 + CAL101 (2.5 μM + 2.5 μM), or left untreated, for 24 h. Cells were then stained with Annexin V-FITC/PI and flow cytometry analysis was carried-out to evaluate the induction of apoptosis. Q1 quadrant (FITC − /PI + ); Q2 quadrant (FITC + /PI + ); Q3 quadrant (FITC − /PI − ); Q4 quadrant (FITC + /PI − ). ( D ) Caspase-3 activity assay in RD, A204 and SJCRH30 cells, treated with 5 μM of either BYL-719, CAL-101 or BYL-719 + CAL-101 (2.5 μM + 2.5 μM), or left untreated for 48 h. Fifty (50) μg of protein lysate for each experimental point was used in the analysis of endogenous caspase-3 activity. Samples were analyzed in triplicate and data are representative of the combined biological experiments. ** p < 0.01; *** p < 0.001. ( E ) Western blot analysis of RD cells to detect the activation of caspase-3 and caspase-7 after treatment with 5 μM of either BYL-719, CAL-101, BYL-719 + CAL-101 (2.5 μM + 2.5 μM) or AZD8835 for 72 h; 60 μg of protein was blotted in each lane. Antibody to β-actin served as a loading control.
    Figure Legend Snippet: Combination of BYL-719 + CAL-101 arrests rhabdomyosarcoma cell cycle and induces caspase-dependent apoptosis. ( A ) RD, SJCRH30, and A204 cells were seeded in a 6-well plate at a concentration of 2 × 10 5 cells/well. Cells were treated with 5 μM of either Alpelisib (BYL-719), Idelalisib (CAL-101), AZD8835 or BYL-719 + CAL-101 (2.5 μM + 2.5 μM), or left untreated. After 24, 48, and 72 h, cells were collected and counted. Cell viability was assessed by trypan blue exclusion. Histograms show the percentage of viable cells and are representative of three independent experiments. *** p < 0.001. ( B ) Cell cycle analysis of RD, A204 and SJCRH30 cells treated with 5 μM of either BYL-719, CAL-101, AZD8835 or BYL-719 + CAL-101 (2.5 μM + 2.5 μM), or left untreated for 72 h. RD, SJCRH30, and A204 were seeded at the concentration of 1 × 10 6 cells/well; after 24 h, cells were treated as described above, stained with propidium iodide (PI) and subjected to FACS analysis. Histograms show the percent distribution of cells in each of the cell cycle phases and are representative of three independent experiments. ( C ) RD, SJCRH30, and A204 were seeded at the concentration of 1 × 10 6 cells in 100 mm plates. After an overnight incubation, cells were treated with 5 μM of either BYL-719, CAL101, AZD8835, or BYL-719 + CAL101 (2.5 μM + 2.5 μM), or left untreated, for 24 h. Cells were then stained with Annexin V-FITC/PI and flow cytometry analysis was carried-out to evaluate the induction of apoptosis. Q1 quadrant (FITC − /PI + ); Q2 quadrant (FITC + /PI + ); Q3 quadrant (FITC − /PI − ); Q4 quadrant (FITC + /PI − ). ( D ) Caspase-3 activity assay in RD, A204 and SJCRH30 cells, treated with 5 μM of either BYL-719, CAL-101 or BYL-719 + CAL-101 (2.5 μM + 2.5 μM), or left untreated for 48 h. Fifty (50) μg of protein lysate for each experimental point was used in the analysis of endogenous caspase-3 activity. Samples were analyzed in triplicate and data are representative of the combined biological experiments. ** p < 0.01; *** p < 0.001. ( E ) Western blot analysis of RD cells to detect the activation of caspase-3 and caspase-7 after treatment with 5 μM of either BYL-719, CAL-101, BYL-719 + CAL-101 (2.5 μM + 2.5 μM) or AZD8835 for 72 h; 60 μg of protein was blotted in each lane. Antibody to β-actin served as a loading control.

    Techniques Used: Concentration Assay, Cell Cycle Assay, Staining, Incubation, Flow Cytometry, Caspase-3 Activity Assay, Activity Assay, Western Blot, Activation Assay

    PI3K p110α inhibitors down-modulate Akt signaling. RD, SJCRH30, and A204 cells were seeded at a concentration of 3 × 10 6 cells in T75 flasks; after an overnight incubation, cells were treated with 5 μM of either BYL-719, CAL101 or BYL-719 + CAL101 (2.5 μM + 2.5 μM), or left untreated, for 24 h. Cells were collected, lysed and protein lysates were subjected to immunoblotting.
    Figure Legend Snippet: PI3K p110α inhibitors down-modulate Akt signaling. RD, SJCRH30, and A204 cells were seeded at a concentration of 3 × 10 6 cells in T75 flasks; after an overnight incubation, cells were treated with 5 μM of either BYL-719, CAL101 or BYL-719 + CAL101 (2.5 μM + 2.5 μM), or left untreated, for 24 h. Cells were collected, lysed and protein lysates were subjected to immunoblotting.

    Techniques Used: Concentration Assay, Incubation, Western Blot

    a 204 cells atcc htb 82  (ATCC)


    Bioz Verified Symbol ATCC is a verified supplier
    Bioz Manufacturer Symbol ATCC manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 99

    Structured Review

    ATCC a 204 cells atcc htb 82
    A 204 Cells Atcc Htb 82, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/a 204 cells atcc htb 82/product/ATCC
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    a 204 cells atcc htb 82 - by Bioz Stars, 2024-05
    99/100 stars

    Images

    a 204 female cells  (ATCC)


    Bioz Verified Symbol ATCC is a verified supplier
    Bioz Manufacturer Symbol ATCC manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 99

    Structured Review

    ATCC a 204 female cells
    A 204 Female Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/a 204 female cells/product/ATCC
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    a 204 female cells - by Bioz Stars, 2024-05
    99/100 stars

    Images

    Similar Products

  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • a204  (ATCC)
    99
    ATCC a204
    IC 50 values for ATRA, 4-HPR, and derivatives on various tumor cell lines
    A204, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/a204/product/ATCC
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    a204 - by Bioz Stars, 2024-05
    99/100 stars
      Buy from Supplier

    86
    ATCC htb 82 rrid cvcl 1058 human
    IC 50 values for ATRA, 4-HPR, and derivatives on various tumor cell lines
    Htb 82 Rrid Cvcl 1058 Human, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/htb 82 rrid cvcl 1058 human/product/ATCC
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    htb 82 rrid cvcl 1058 human - by Bioz Stars, 2024-05
    86/100 stars
      Buy from Supplier

    86
    ATCC a 172 crl 1620 glioblastoma 1 067 a 204 htb 82 rhabdomyosarcoma 1 360 a375 crl
    IC 50 values for ATRA, 4-HPR, and derivatives on various tumor cell lines
    A 172 Crl 1620 Glioblastoma 1 067 A 204 Htb 82 Rhabdomyosarcoma 1 360 A375 Crl, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/a 172 crl 1620 glioblastoma 1 067 a 204 htb 82 rhabdomyosarcoma 1 360 a375 crl/product/ATCC
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    a 172 crl 1620 glioblastoma 1 067 a 204 htb 82 rhabdomyosarcoma 1 360 a375 crl - by Bioz Stars, 2024-05
    86/100 stars
      Buy from Supplier

    99
    ATCC htb82 erms cell lines
    Formation of holoclones and spheres in RMS cell lines. (a) Representative images of holoclones, meroclones, and paraclones in CW9019 and <t>HTB82</t> cell lines. (b) Percentages of clones classified as holoclones, meroclones, or paraclones in CW9019, HTB82, RD, and RH30 cell lines. (c) Representative images of CW9019 and HTB82 spheres. (d) Number of spheres formed per 1000 cells seeded in neurosphere media. Data were expressed as mean ± SEM of three independent experiments.
    Htb82 Erms Cell Lines, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/htb82 erms cell lines/product/ATCC
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    htb82 erms cell lines - by Bioz Stars, 2024-05
    99/100 stars
      Buy from Supplier

    95
    ATCC j 82 htb 1
    Formation of holoclones and spheres in RMS cell lines. (a) Representative images of holoclones, meroclones, and paraclones in CW9019 and <t>HTB82</t> cell lines. (b) Percentages of clones classified as holoclones, meroclones, or paraclones in CW9019, HTB82, RD, and RH30 cell lines. (c) Representative images of CW9019 and HTB82 spheres. (d) Number of spheres formed per 1000 cells seeded in neurosphere media. Data were expressed as mean ± SEM of three independent experiments.
    J 82 Htb 1, supplied by ATCC, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/j 82 htb 1/product/ATCC
    Average 95 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    j 82 htb 1 - by Bioz Stars, 2024-05
    95/100 stars
      Buy from Supplier

    99
    ATCC a204 cells
    Inhibition of rhabdoid tumor growth by flavopiridol and 4OH-Tam . A-C . Survival curves of MON (A), G401 (B), and <t>A204</t> (C) cells treated with flavopiridol in the presence and absence of 4OH-Tam for two days. D . Table of IC50 values as approximated from A-C. Flavo = flavopiridol; 4OHT = 4OH-Tam.
    A204 Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/a204 cells/product/ATCC
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    a204 cells - by Bioz Stars, 2024-05
    99/100 stars
      Buy from Supplier

    a 204  (ATCC)
    99
    ATCC a 204
    MYOD1+ NOG+ cells predominant survival in RD and <t>A-204</t> cell cultures after treatment with either Vincristine or Doxorubicin. (a) Plots show examples of flow analysis of RD and A-204 cells treated with Vincristine and Doxorubicin. In grey is highlighted the quadrant representing MYOD1+ NOG+ cells. (b) Percentage of live MYOD1+ NOG+ phenotype cells in RD and A-204 cell cultures after 2 days of treatment either with 1 and 10 nM of Vincristine (top panel) or with 0.1 and 1 μ M of Doxorubicin (bottom panel). (c) Absolute numbers of total live cells in RD and A-204 cultures treated with 10 nM of Vincristine or 1 μ M of Doxorubicin for 2 days. Data are presented as a ratio of absolute numbers of cells in treated cultures to absolute numbers of cells in nontreated cultures (fold change). Data are shown as mean ± standard deviation ( N = 11). ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001, ANOVA with Tukey's multiple comparison test.
    A 204, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/a 204/product/ATCC
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    a 204 - by Bioz Stars, 2024-05
    99/100 stars
      Buy from Supplier

    99
    ATCC a 204 cells atcc htb 82
    MYOD1+ NOG+ cells predominant survival in RD and <t>A-204</t> cell cultures after treatment with either Vincristine or Doxorubicin. (a) Plots show examples of flow analysis of RD and A-204 cells treated with Vincristine and Doxorubicin. In grey is highlighted the quadrant representing MYOD1+ NOG+ cells. (b) Percentage of live MYOD1+ NOG+ phenotype cells in RD and A-204 cell cultures after 2 days of treatment either with 1 and 10 nM of Vincristine (top panel) or with 0.1 and 1 μ M of Doxorubicin (bottom panel). (c) Absolute numbers of total live cells in RD and A-204 cultures treated with 10 nM of Vincristine or 1 μ M of Doxorubicin for 2 days. Data are presented as a ratio of absolute numbers of cells in treated cultures to absolute numbers of cells in nontreated cultures (fold change). Data are shown as mean ± standard deviation ( N = 11). ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001, ANOVA with Tukey's multiple comparison test.
    A 204 Cells Atcc Htb 82, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/a 204 cells atcc htb 82/product/ATCC
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    a 204 cells atcc htb 82 - by Bioz Stars, 2024-05
    99/100 stars
      Buy from Supplier

    99
    ATCC a 204 female cells
    MYOD1+ NOG+ cells predominant survival in RD and <t>A-204</t> cell cultures after treatment with either Vincristine or Doxorubicin. (a) Plots show examples of flow analysis of RD and A-204 cells treated with Vincristine and Doxorubicin. In grey is highlighted the quadrant representing MYOD1+ NOG+ cells. (b) Percentage of live MYOD1+ NOG+ phenotype cells in RD and A-204 cell cultures after 2 days of treatment either with 1 and 10 nM of Vincristine (top panel) or with 0.1 and 1 μ M of Doxorubicin (bottom panel). (c) Absolute numbers of total live cells in RD and A-204 cultures treated with 10 nM of Vincristine or 1 μ M of Doxorubicin for 2 days. Data are presented as a ratio of absolute numbers of cells in treated cultures to absolute numbers of cells in nontreated cultures (fold change). Data are shown as mean ± standard deviation ( N = 11). ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001, ANOVA with Tukey's multiple comparison test.
    A 204 Female Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/a 204 female cells/product/ATCC
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    a 204 female cells - by Bioz Stars, 2024-05
    99/100 stars
      Buy from Supplier

    Image Search Results


    IC 50 values for ATRA, 4-HPR, and derivatives on various tumor cell lines

    Journal: Cancer Cell International

    Article Title: In vitro activities of novel 4-HPR derivatives on a panel of rhabdoid and other tumor cell lines

    doi: 10.1186/1475-2867-11-34

    Figure Lengend Snippet: IC 50 values for ATRA, 4-HPR, and derivatives on various tumor cell lines

    Article Snippet: The RT cell lines used, MON [ ], G401 (American Type Culture Collection), and A204 (American Type Culture Collection) were maintained in RPMI supplemented with 10% fetal bovine serum, 50 U/mL penicillin, 50 μg/mL streptomycin, and 2 mM L-glutamine.

    Techniques:

    Inhibition of RT cell growth by 4-HPR and its derivatives and peptidomimetics : Survival curves of MON (A), G401 (B) and A204 (C) cells treated for three days with increasing concentrations of ATRA, 4-HPR, the most active halogen substituted derivative of 4-HPR (5h and 5j) and peptidomimetic compounds (11a, 11c and 11d).

    Journal: Cancer Cell International

    Article Title: In vitro activities of novel 4-HPR derivatives on a panel of rhabdoid and other tumor cell lines

    doi: 10.1186/1475-2867-11-34

    Figure Lengend Snippet: Inhibition of RT cell growth by 4-HPR and its derivatives and peptidomimetics : Survival curves of MON (A), G401 (B) and A204 (C) cells treated for three days with increasing concentrations of ATRA, 4-HPR, the most active halogen substituted derivative of 4-HPR (5h and 5j) and peptidomimetic compounds (11a, 11c and 11d).

    Article Snippet: The RT cell lines used, MON [ ], G401 (American Type Culture Collection), and A204 (American Type Culture Collection) were maintained in RPMI supplemented with 10% fetal bovine serum, 50 U/mL penicillin, 50 μg/mL streptomycin, and 2 mM L-glutamine.

    Techniques: Inhibition

    Induction of cell cycle arrest by 4-HPR and its derivatives and peptidomimetics : A-C. Cell cycle profile of MON (A), G401 (B), and A204 (C) cells treated for three days with 5 μM or 10 μM concentrations of ATRA, 4-HPR, and the iodo-conjugated 4-HPR derivative (5j) and peptidomimetic (11d).

    Journal: Cancer Cell International

    Article Title: In vitro activities of novel 4-HPR derivatives on a panel of rhabdoid and other tumor cell lines

    doi: 10.1186/1475-2867-11-34

    Figure Lengend Snippet: Induction of cell cycle arrest by 4-HPR and its derivatives and peptidomimetics : A-C. Cell cycle profile of MON (A), G401 (B), and A204 (C) cells treated for three days with 5 μM or 10 μM concentrations of ATRA, 4-HPR, and the iodo-conjugated 4-HPR derivative (5j) and peptidomimetic (11d).

    Article Snippet: The RT cell lines used, MON [ ], G401 (American Type Culture Collection), and A204 (American Type Culture Collection) were maintained in RPMI supplemented with 10% fetal bovine serum, 50 U/mL penicillin, 50 μg/mL streptomycin, and 2 mM L-glutamine.

    Techniques:

    Induction of cell death by 4-HPR and its derivatives and peptidomimetics : A-C. Percentage of MON (A), G401 (B), and A204 (C) cells at sub- G 1 when treated for three days with 5 μM or 10 μM concentrations of ATRA, 4-HPR, and the iodo-conjugated 4-HPR derivative (5j) and peptidomimetic (11d).

    Journal: Cancer Cell International

    Article Title: In vitro activities of novel 4-HPR derivatives on a panel of rhabdoid and other tumor cell lines

    doi: 10.1186/1475-2867-11-34

    Figure Lengend Snippet: Induction of cell death by 4-HPR and its derivatives and peptidomimetics : A-C. Percentage of MON (A), G401 (B), and A204 (C) cells at sub- G 1 when treated for three days with 5 μM or 10 μM concentrations of ATRA, 4-HPR, and the iodo-conjugated 4-HPR derivative (5j) and peptidomimetic (11d).

    Article Snippet: The RT cell lines used, MON [ ], G401 (American Type Culture Collection), and A204 (American Type Culture Collection) were maintained in RPMI supplemented with 10% fetal bovine serum, 50 U/mL penicillin, 50 μg/mL streptomycin, and 2 mM L-glutamine.

    Techniques:

    Formation of holoclones and spheres in RMS cell lines. (a) Representative images of holoclones, meroclones, and paraclones in CW9019 and HTB82 cell lines. (b) Percentages of clones classified as holoclones, meroclones, or paraclones in CW9019, HTB82, RD, and RH30 cell lines. (c) Representative images of CW9019 and HTB82 spheres. (d) Number of spheres formed per 1000 cells seeded in neurosphere media. Data were expressed as mean ± SEM of three independent experiments.

    Journal: Stem Cells International

    Article Title: Hedgehog Pathway Inhibition Hampers Sphere and Holoclone Formation in Rhabdomyosarcoma

    doi: 10.1155/2017/7507380

    Figure Lengend Snippet: Formation of holoclones and spheres in RMS cell lines. (a) Representative images of holoclones, meroclones, and paraclones in CW9019 and HTB82 cell lines. (b) Percentages of clones classified as holoclones, meroclones, or paraclones in CW9019, HTB82, RD, and RH30 cell lines. (c) Representative images of CW9019 and HTB82 spheres. (d) Number of spheres formed per 1000 cells seeded in neurosphere media. Data were expressed as mean ± SEM of three independent experiments.

    Article Snippet: RH30 (aRMS, PAX3/FOXO1 translocation), RD (eRMS), and HTB82 (eRMS) cell lines were obtained from American Type Culture Collection (ATCC) and CW9019 (aRMS, PAX7/FOXO1 translocation) was generated in Dr. Jaclyn Biegel's laboratory.

    Techniques: Clone Assay

    Self-renewal capacity of cells contained in RMS holoclones and spheres. ((a) and (b)) Initial percentage of the three types of clones generated in CW9019 and HTB82 cell lines, respectively. Representative images and quantification of secondary clones formed from holoclones (c and d), meroclones (e and f), and paraclones (g and h) in CW9019 and HTB82 cell lines, respectively. Enrichment in the secondary sphere fraction obtained in CW9019 (i) and HTB82 (j) cell lines. All experiments were tested in triplicate. Significance: ∗ p < 0.05; ∗∗ p < 0.01.

    Journal: Stem Cells International

    Article Title: Hedgehog Pathway Inhibition Hampers Sphere and Holoclone Formation in Rhabdomyosarcoma

    doi: 10.1155/2017/7507380

    Figure Lengend Snippet: Self-renewal capacity of cells contained in RMS holoclones and spheres. ((a) and (b)) Initial percentage of the three types of clones generated in CW9019 and HTB82 cell lines, respectively. Representative images and quantification of secondary clones formed from holoclones (c and d), meroclones (e and f), and paraclones (g and h) in CW9019 and HTB82 cell lines, respectively. Enrichment in the secondary sphere fraction obtained in CW9019 (i) and HTB82 (j) cell lines. All experiments were tested in triplicate. Significance: ∗ p < 0.05; ∗∗ p < 0.01.

    Article Snippet: RH30 (aRMS, PAX3/FOXO1 translocation), RD (eRMS), and HTB82 (eRMS) cell lines were obtained from American Type Culture Collection (ATCC) and CW9019 (aRMS, PAX7/FOXO1 translocation) was generated in Dr. Jaclyn Biegel's laboratory.

    Techniques: Clone Assay, Generated

    RMS stem-like cells showed GLI1 upregulation. Analysis of SHH, IHH, DHH, and GLI1 mRNA levels in holoclones (a, b, c, and d) and spheres (e, f, g, and h) of CW9010 and HTB82 cell lines. Values were referred to expression levels of meroclones and adherent cells (black bars), respectively. Data were tested in triplicate and expressed as mean ± SEM. Significance: ∗ p < 0.05; ∗∗ p < 0.01; ∗∗∗ p < 0.001.

    Journal: Stem Cells International

    Article Title: Hedgehog Pathway Inhibition Hampers Sphere and Holoclone Formation in Rhabdomyosarcoma

    doi: 10.1155/2017/7507380

    Figure Lengend Snippet: RMS stem-like cells showed GLI1 upregulation. Analysis of SHH, IHH, DHH, and GLI1 mRNA levels in holoclones (a, b, c, and d) and spheres (e, f, g, and h) of CW9010 and HTB82 cell lines. Values were referred to expression levels of meroclones and adherent cells (black bars), respectively. Data were tested in triplicate and expressed as mean ± SEM. Significance: ∗ p < 0.05; ∗∗ p < 0.01; ∗∗∗ p < 0.001.

    Article Snippet: RH30 (aRMS, PAX3/FOXO1 translocation), RD (eRMS), and HTB82 (eRMS) cell lines were obtained from American Type Culture Collection (ATCC) and CW9019 (aRMS, PAX7/FOXO1 translocation) was generated in Dr. Jaclyn Biegel's laboratory.

    Techniques: Expressing

    Effects of HH ligands and GLI1 depletion on RMS holoclone and sphere formation. ((a) and (b)) Plots representing the percentage of each colony type formed by SHH , IHH , DHH , and GLI1 shRNA-expressing CW9010 and HTB82 cells, respectively. ((c) and (d)) Plots representing the relative diameter of spheres formed by shRNA-expressing cells. Values were referred to control cells (transfected with pGIPZ empty vector) and expressed as mean ± SEM of three independent experiments. From (e) to (h) real-time PCRs to assess the downregulation of all shRNA targets: SHH, IHH, DHH , and GLI1 , respectively. Significance: ∗ p < 0.05; ∗∗ p < 0.01; ∗∗∗ p < 0.001.

    Journal: Stem Cells International

    Article Title: Hedgehog Pathway Inhibition Hampers Sphere and Holoclone Formation in Rhabdomyosarcoma

    doi: 10.1155/2017/7507380

    Figure Lengend Snippet: Effects of HH ligands and GLI1 depletion on RMS holoclone and sphere formation. ((a) and (b)) Plots representing the percentage of each colony type formed by SHH , IHH , DHH , and GLI1 shRNA-expressing CW9010 and HTB82 cells, respectively. ((c) and (d)) Plots representing the relative diameter of spheres formed by shRNA-expressing cells. Values were referred to control cells (transfected with pGIPZ empty vector) and expressed as mean ± SEM of three independent experiments. From (e) to (h) real-time PCRs to assess the downregulation of all shRNA targets: SHH, IHH, DHH , and GLI1 , respectively. Significance: ∗ p < 0.05; ∗∗ p < 0.01; ∗∗∗ p < 0.001.

    Article Snippet: RH30 (aRMS, PAX3/FOXO1 translocation), RD (eRMS), and HTB82 (eRMS) cell lines were obtained from American Type Culture Collection (ATCC) and CW9019 (aRMS, PAX7/FOXO1 translocation) was generated in Dr. Jaclyn Biegel's laboratory.

    Techniques: shRNA, Expressing, Transfection, Plasmid Preparation

    HH pathway inhibitors hindered holoclone and sphere formation. CW9019 and HTB82 cells were pretreated with Sonidegib ( SMO inhibitor) and MEDI-5304 (HH ligand blocking antibody) 48 h prior to holoclone- and sphere-formation assays. ((a) and (b)) Plots representing the percentage of each colony type formed after HH pathway inhibitor treatment. Percentages of number (c and d) and diameter (e and f) of spheres formed after HH pathway inhibitor treatment. Values were referred to control cells (treated with vehicle) and expressed as mean ± SEM of three independent experiments. Significance: ∗ p < 0.05; ∗∗ p < 0.01; referred to holoclones of control cells and # p < 0.05; ## p < 0.01 referred to paraclones of control cells.

    Journal: Stem Cells International

    Article Title: Hedgehog Pathway Inhibition Hampers Sphere and Holoclone Formation in Rhabdomyosarcoma

    doi: 10.1155/2017/7507380

    Figure Lengend Snippet: HH pathway inhibitors hindered holoclone and sphere formation. CW9019 and HTB82 cells were pretreated with Sonidegib ( SMO inhibitor) and MEDI-5304 (HH ligand blocking antibody) 48 h prior to holoclone- and sphere-formation assays. ((a) and (b)) Plots representing the percentage of each colony type formed after HH pathway inhibitor treatment. Percentages of number (c and d) and diameter (e and f) of spheres formed after HH pathway inhibitor treatment. Values were referred to control cells (treated with vehicle) and expressed as mean ± SEM of three independent experiments. Significance: ∗ p < 0.05; ∗∗ p < 0.01; referred to holoclones of control cells and # p < 0.05; ## p < 0.01 referred to paraclones of control cells.

    Article Snippet: RH30 (aRMS, PAX3/FOXO1 translocation), RD (eRMS), and HTB82 (eRMS) cell lines were obtained from American Type Culture Collection (ATCC) and CW9019 (aRMS, PAX7/FOXO1 translocation) was generated in Dr. Jaclyn Biegel's laboratory.

    Techniques: Blocking Assay

    Holoclones showed induction of stem cell markers. mRNA levels of OCT4, NANOG, PAX7, and KITLG were evaluated in CW9019 and HTB82 cell lines. The expression levels of these markers were evaluated by conventional RT-PCR in total cell lines (line) and holoclones of first (holo1) and second (holo2) passages.

    Journal: Stem Cells International

    Article Title: Hedgehog Pathway Inhibition Hampers Sphere and Holoclone Formation in Rhabdomyosarcoma

    doi: 10.1155/2017/7507380

    Figure Lengend Snippet: Holoclones showed induction of stem cell markers. mRNA levels of OCT4, NANOG, PAX7, and KITLG were evaluated in CW9019 and HTB82 cell lines. The expression levels of these markers were evaluated by conventional RT-PCR in total cell lines (line) and holoclones of first (holo1) and second (holo2) passages.

    Article Snippet: RH30 (aRMS, PAX3/FOXO1 translocation), RD (eRMS), and HTB82 (eRMS) cell lines were obtained from American Type Culture Collection (ATCC) and CW9019 (aRMS, PAX7/FOXO1 translocation) was generated in Dr. Jaclyn Biegel's laboratory.

    Techniques: Expressing, Reverse Transcription Polymerase Chain Reaction

    Inhibition of rhabdoid tumor growth by flavopiridol and 4OH-Tam . A-C . Survival curves of MON (A), G401 (B), and A204 (C) cells treated with flavopiridol in the presence and absence of 4OH-Tam for two days. D . Table of IC50 values as approximated from A-C. Flavo = flavopiridol; 4OHT = 4OH-Tam.

    Journal: BMC Cancer

    Article Title: Potent inhibition of rhabdoid tumor cells by combination of flavopiridol and 4OH-tamoxifen

    doi: 10.1186/1471-2407-10-634

    Figure Lengend Snippet: Inhibition of rhabdoid tumor growth by flavopiridol and 4OH-Tam . A-C . Survival curves of MON (A), G401 (B), and A204 (C) cells treated with flavopiridol in the presence and absence of 4OH-Tam for two days. D . Table of IC50 values as approximated from A-C. Flavo = flavopiridol; 4OHT = 4OH-Tam.

    Article Snippet: MON [ ], G401 (American Type Culture Collection), and A204 cells (American Type Culture Collection) were maintained in RPMI supplemented with 10% fetal bovine serum, 50 U/ml penicillin, 50 μg/ml streptomycin, and 2 mM L-glutamine.

    Techniques: Inhibition

    MYOD1+ NOG+ cells predominant survival in RD and A-204 cell cultures after treatment with either Vincristine or Doxorubicin. (a) Plots show examples of flow analysis of RD and A-204 cells treated with Vincristine and Doxorubicin. In grey is highlighted the quadrant representing MYOD1+ NOG+ cells. (b) Percentage of live MYOD1+ NOG+ phenotype cells in RD and A-204 cell cultures after 2 days of treatment either with 1 and 10 nM of Vincristine (top panel) or with 0.1 and 1 μ M of Doxorubicin (bottom panel). (c) Absolute numbers of total live cells in RD and A-204 cultures treated with 10 nM of Vincristine or 1 μ M of Doxorubicin for 2 days. Data are presented as a ratio of absolute numbers of cells in treated cultures to absolute numbers of cells in nontreated cultures (fold change). Data are shown as mean ± standard deviation ( N = 11). ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001, ANOVA with Tukey's multiple comparison test.

    Journal: Sarcoma

    Article Title: Induction of Myogenic Differentiation Improves Chemosensitivity of Chemoresistant Cells in Soft-Tissue Sarcoma Cell Lines

    doi: 10.1155/2020/8647981

    Figure Lengend Snippet: MYOD1+ NOG+ cells predominant survival in RD and A-204 cell cultures after treatment with either Vincristine or Doxorubicin. (a) Plots show examples of flow analysis of RD and A-204 cells treated with Vincristine and Doxorubicin. In grey is highlighted the quadrant representing MYOD1+ NOG+ cells. (b) Percentage of live MYOD1+ NOG+ phenotype cells in RD and A-204 cell cultures after 2 days of treatment either with 1 and 10 nM of Vincristine (top panel) or with 0.1 and 1 μ M of Doxorubicin (bottom panel). (c) Absolute numbers of total live cells in RD and A-204 cultures treated with 10 nM of Vincristine or 1 μ M of Doxorubicin for 2 days. Data are presented as a ratio of absolute numbers of cells in treated cultures to absolute numbers of cells in nontreated cultures (fold change). Data are shown as mean ± standard deviation ( N = 11). ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001, ANOVA with Tukey's multiple comparison test.

    Article Snippet: RD (ATCC® CCL-136™) and A-204 (ATCC® HTB-82™) were purchased from American Type Culture Collection (Manassas, VA) and cultured under standard conditions (37°C, 5% CO 2 ) in DMEM High Glucose supplemented with 10% fetal bovine serum.

    Techniques: Standard Deviation

    MYOD1+ NOG+ cells express the highest levels of antiapoptotic BCL2 protein among other cells in RD and A-204 cultures. Median fluorescence intensity (MFI) of BCL2 protein in MYOD1/NOG cell subpopulations of RD (left plot) and A-204 (right plot) cell lines. Data are presented as a ratio of BCL2 MFI of MYOD1+ NOG+ cells and MYOD1− NOG+ cells to BCL2 MFI of MYOD1− NOG− cells (fold change), (mean ± standard deviation, N = 9). ∗ p < 0.05, ∗∗ p < 0.01, ANOVA with Tukey's multiple comparison test).

    Journal: Sarcoma

    Article Title: Induction of Myogenic Differentiation Improves Chemosensitivity of Chemoresistant Cells in Soft-Tissue Sarcoma Cell Lines

    doi: 10.1155/2020/8647981

    Figure Lengend Snippet: MYOD1+ NOG+ cells express the highest levels of antiapoptotic BCL2 protein among other cells in RD and A-204 cultures. Median fluorescence intensity (MFI) of BCL2 protein in MYOD1/NOG cell subpopulations of RD (left plot) and A-204 (right plot) cell lines. Data are presented as a ratio of BCL2 MFI of MYOD1+ NOG+ cells and MYOD1− NOG+ cells to BCL2 MFI of MYOD1− NOG− cells (fold change), (mean ± standard deviation, N = 9). ∗ p < 0.05, ∗∗ p < 0.01, ANOVA with Tukey's multiple comparison test).

    Article Snippet: RD (ATCC® CCL-136™) and A-204 (ATCC® HTB-82™) were purchased from American Type Culture Collection (Manassas, VA) and cultured under standard conditions (37°C, 5% CO 2 ) in DMEM High Glucose supplemented with 10% fetal bovine serum.

    Techniques: Fluorescence, Standard Deviation

    Differentiation therapy increases the effectiveness of chemotherapy. Absolute numbers of total live cells RD (a) and A-204 (b) in cultures treated for 6 days with TPA/GSK126, 10 nM of Vincristine, or combination of Vincristine and TPA/GSK126. Data are presented as a ratio of absolute numbers of cells in treated cultures to absolute numbers of cells in nontreated cultures (fold change) (mean ± standard deviation, N = 4). ∗ p < 0.05, ∗∗∗ p < 0.001, ANOVA with Tukey's multiple comparison test for the analysis between the groups.

    Journal: Sarcoma

    Article Title: Induction of Myogenic Differentiation Improves Chemosensitivity of Chemoresistant Cells in Soft-Tissue Sarcoma Cell Lines

    doi: 10.1155/2020/8647981

    Figure Lengend Snippet: Differentiation therapy increases the effectiveness of chemotherapy. Absolute numbers of total live cells RD (a) and A-204 (b) in cultures treated for 6 days with TPA/GSK126, 10 nM of Vincristine, or combination of Vincristine and TPA/GSK126. Data are presented as a ratio of absolute numbers of cells in treated cultures to absolute numbers of cells in nontreated cultures (fold change) (mean ± standard deviation, N = 4). ∗ p < 0.05, ∗∗∗ p < 0.001, ANOVA with Tukey's multiple comparison test for the analysis between the groups.

    Article Snippet: RD (ATCC® CCL-136™) and A-204 (ATCC® HTB-82™) were purchased from American Type Culture Collection (Manassas, VA) and cultured under standard conditions (37°C, 5% CO 2 ) in DMEM High Glucose supplemented with 10% fetal bovine serum.

    Techniques: Standard Deviation