atcc htb 38 ovcar  (ATCC)


Bioz Verified Symbol ATCC is a verified supplier
Bioz Manufacturer Symbol ATCC manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 86

    Structured Review

    ATCC atcc htb 38 ovcar
    Atcc Htb 38 Ovcar, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/atcc htb 38 ovcar/product/ATCC
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    atcc htb 38 ovcar - by Bioz Stars, 2024-05
    86/100 stars

    Images

    ht 29 htb 38 cells  (ATCC)


    Bioz Verified Symbol ATCC is a verified supplier
    Bioz Manufacturer Symbol ATCC manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 86

    Structured Review

    ATCC ht 29 htb 38 cells
    Biodistribution of [ 89 Zr]-DFO(0.7)-RW03 IgG , [ 89 Zr]-DFO(3.0)-RW03 IgG , [ 89 Zr]-DFO(2.9)-RW03 scFv-Fc in <t>HT-29</t> xenograft BALB/c nu/nu mice. ( a ) [ 89 Zr]-DFO(0.7)-RW03 IgG (0.16 ± 0.02 MBq, 5 ± 0.7 ug) at 24, 48, 96 h p.i., ( b ) [ 89 Zr]-DFO(3.0)-RW03 IgG (0.14 ± 0.03 MBq, 1 ± 0.3 µg) at 24, 48, 96 h p.i., ( c ) [ 89 Zr]-DFO(2.9)-RW03 scFv-Fc (0.085 ± 0.02 MBq, 1 ± 0.2 µg) at 24, 48, and 72 h p.i. The blocked cohort received [ 89 Zr]-DFO-RW03 scFv-Fc (0.09 ± 0.02 MBq, 1.0 ± 0.2 µg) and excess RW03 IgG (400 µg). Uptake is expressed as mean percentage injected dose per gram tissue (%ID/g) ± SEM.
    Ht 29 Htb 38 Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ht 29 htb 38 cells/product/ATCC
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    ht 29 htb 38 cells - by Bioz Stars, 2024-05
    86/100 stars

    Images

    1) Product Images from "89 Zr-labeled ImmunoPET targeting the cancer stem cell antigen CD133 using fully-human antibody constructs"

    Article Title: 89 Zr-labeled ImmunoPET targeting the cancer stem cell antigen CD133 using fully-human antibody constructs

    Journal: EJNMMI Research

    doi: 10.1186/s13550-024-01091-9

    Biodistribution of [ 89 Zr]-DFO(0.7)-RW03 IgG , [ 89 Zr]-DFO(3.0)-RW03 IgG , [ 89 Zr]-DFO(2.9)-RW03 scFv-Fc in HT-29 xenograft BALB/c nu/nu mice. ( a ) [ 89 Zr]-DFO(0.7)-RW03 IgG (0.16 ± 0.02 MBq, 5 ± 0.7 ug) at 24, 48, 96 h p.i., ( b ) [ 89 Zr]-DFO(3.0)-RW03 IgG (0.14 ± 0.03 MBq, 1 ± 0.3 µg) at 24, 48, 96 h p.i., ( c ) [ 89 Zr]-DFO(2.9)-RW03 scFv-Fc (0.085 ± 0.02 MBq, 1 ± 0.2 µg) at 24, 48, and 72 h p.i. The blocked cohort received [ 89 Zr]-DFO-RW03 scFv-Fc (0.09 ± 0.02 MBq, 1.0 ± 0.2 µg) and excess RW03 IgG (400 µg). Uptake is expressed as mean percentage injected dose per gram tissue (%ID/g) ± SEM.
    Figure Legend Snippet: Biodistribution of [ 89 Zr]-DFO(0.7)-RW03 IgG , [ 89 Zr]-DFO(3.0)-RW03 IgG , [ 89 Zr]-DFO(2.9)-RW03 scFv-Fc in HT-29 xenograft BALB/c nu/nu mice. ( a ) [ 89 Zr]-DFO(0.7)-RW03 IgG (0.16 ± 0.02 MBq, 5 ± 0.7 ug) at 24, 48, 96 h p.i., ( b ) [ 89 Zr]-DFO(3.0)-RW03 IgG (0.14 ± 0.03 MBq, 1 ± 0.3 µg) at 24, 48, 96 h p.i., ( c ) [ 89 Zr]-DFO(2.9)-RW03 scFv-Fc (0.085 ± 0.02 MBq, 1 ± 0.2 µg) at 24, 48, and 72 h p.i. The blocked cohort received [ 89 Zr]-DFO-RW03 scFv-Fc (0.09 ± 0.02 MBq, 1.0 ± 0.2 µg) and excess RW03 IgG (400 µg). Uptake is expressed as mean percentage injected dose per gram tissue (%ID/g) ± SEM.

    Techniques Used: Injection

    In vivo PET images ( a,c,e ) and corresponding ROI analysis (b, d, f) of [ 89 Zr]-RW03 based radioligands: ( a & b ) [ 89 Zr]-DFO(0.7)-RW03 IgG (1.27 ± 0.03 MBq, 10 ± 0.2 ug), ( c & d ) [ 89 Zr]-DFO(3.0)-RW03 IgG (5.2 ± 0.4 MBq, 27 ± 2 µg), ( e & f ) [ 89 Zr]-DFO(2.9)-RW03 scFv − Fc (1.2 ± 0.1 MBq, 16 ± 2 µg) in HT-29 xenograft BALB/c nu/nu mice. For PET images ( a, c, e ), the coronal plane is presented as a maximum intensity projection (MIP). White arrow = tumor. For ROI analysis ( b, d, f ) uptake is expressed as mean radioactivity per mL tissue volume (%ID/cc) ± SEM
    Figure Legend Snippet: In vivo PET images ( a,c,e ) and corresponding ROI analysis (b, d, f) of [ 89 Zr]-RW03 based radioligands: ( a & b ) [ 89 Zr]-DFO(0.7)-RW03 IgG (1.27 ± 0.03 MBq, 10 ± 0.2 ug), ( c & d ) [ 89 Zr]-DFO(3.0)-RW03 IgG (5.2 ± 0.4 MBq, 27 ± 2 µg), ( e & f ) [ 89 Zr]-DFO(2.9)-RW03 scFv − Fc (1.2 ± 0.1 MBq, 16 ± 2 µg) in HT-29 xenograft BALB/c nu/nu mice. For PET images ( a, c, e ), the coronal plane is presented as a maximum intensity projection (MIP). White arrow = tumor. For ROI analysis ( b, d, f ) uptake is expressed as mean radioactivity per mL tissue volume (%ID/cc) ± SEM

    Techniques Used: In Vivo, Radioactivity

    htb 38  (ATCC)


    Bioz Verified Symbol ATCC is a verified supplier
    Bioz Manufacturer Symbol ATCC manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 86

    Structured Review

    ATCC htb 38
    Htb 38, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/htb 38/product/ATCC
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    htb 38 - by Bioz Stars, 2024-05
    86/100 stars

    Images

    htb 38  (ATCC)


    Bioz Verified Symbol ATCC is a verified supplier
    Bioz Manufacturer Symbol ATCC manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 86

    Structured Review

    ATCC htb 38
    Htb 38, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/htb 38/product/ATCC
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    htb 38 - by Bioz Stars, 2024-05
    86/100 stars

    Images

    htb 38  (ATCC)


    Bioz Verified Symbol ATCC is a verified supplier
    Bioz Manufacturer Symbol ATCC manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 86

    Structured Review

    ATCC htb 38
    Htb 38, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/htb 38/product/ATCC
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    htb 38 - by Bioz Stars, 2024-05
    86/100 stars

    Images

    cell line ht 29 htb 38  (ATCC)


    Bioz Verified Symbol ATCC is a verified supplier
    Bioz Manufacturer Symbol ATCC manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 86

    Structured Review

    ATCC cell line ht 29 htb 38
    Cell Line Ht 29 Htb 38, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/cell line ht 29 htb 38/product/ATCC
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    cell line ht 29 htb 38 - by Bioz Stars, 2024-05
    86/100 stars

    Images

    htb 38  (ATCC)


    Bioz Verified Symbol ATCC is a verified supplier
    Bioz Manufacturer Symbol ATCC manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 86

    Structured Review

    ATCC htb 38
    Htb 38, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/htb 38/product/ATCC
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    htb 38 - by Bioz Stars, 2024-05
    86/100 stars

    Images

    htb 38  (ATCC)


    Bioz Verified Symbol ATCC is a verified supplier
    Bioz Manufacturer Symbol ATCC manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 86

    Structured Review

    ATCC htb 38
    Htb 38, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/htb 38/product/ATCC
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    htb 38 - by Bioz Stars, 2024-05
    86/100 stars

    Images

    ht29 htb 38 cell lines  (ATCC)


    Bioz Verified Symbol ATCC is a verified supplier
    Bioz Manufacturer Symbol ATCC manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 86

    Structured Review

    ATCC ht29 htb 38 cell lines
    ARID5A as a RNA-binding protein that stabilized IDO1 mRNAs via binding their 3′ UTRs. A, B, IDO1 expression was quantified in tissues from HCT116-ARID5A overexpression or tissues (A) from the HCT116-ARID5A KO group (B). C, RIP–qPCR analysis was performed with ARID5A antibody and IDO1 3′UTR-specific primers in HCT116-ARID5A vector or OE cell. D, IDO1 expression was quantified in cells from the HCT116-ARID5A overexpression or HCT116-ARID5A KO group. E, Luciferase analysis was performed on HCT116-ARID5A KO or OE cells. F, G, HCT116 or <t>HT29</t> cells expressing ARID5A sgRNA or ARID5A OE at the indicated times in the presence of actinomycin D (1 μg ml −1 ). The remaining IDO1 mRNA levels were determined. C–G, n = 3 independent experiments; one-way analysis of variance (ANOVA) followed by Bonferroni's test (C–G). Data represent mean ± s.d. * p < 0.05, ** p < 0.01, *** p < 0.001.
    Ht29 Htb 38 Cell Lines, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ht29 htb 38 cell lines/product/ATCC
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    ht29 htb 38 cell lines - by Bioz Stars, 2024-05
    86/100 stars

    Images

    1) Product Images from "ARID5A stabilizes Indoleamine 2,3-dioxygenase expression and enhances CAR T cell exhaustion in colorectal cancer"

    Article Title: ARID5A stabilizes Indoleamine 2,3-dioxygenase expression and enhances CAR T cell exhaustion in colorectal cancer

    Journal: Translational Oncology

    doi: 10.1016/j.tranon.2024.101900

    ARID5A as a RNA-binding protein that stabilized IDO1 mRNAs via binding their 3′ UTRs. A, B, IDO1 expression was quantified in tissues from HCT116-ARID5A overexpression or tissues (A) from the HCT116-ARID5A KO group (B). C, RIP–qPCR analysis was performed with ARID5A antibody and IDO1 3′UTR-specific primers in HCT116-ARID5A vector or OE cell. D, IDO1 expression was quantified in cells from the HCT116-ARID5A overexpression or HCT116-ARID5A KO group. E, Luciferase analysis was performed on HCT116-ARID5A KO or OE cells. F, G, HCT116 or HT29 cells expressing ARID5A sgRNA or ARID5A OE at the indicated times in the presence of actinomycin D (1 μg ml −1 ). The remaining IDO1 mRNA levels were determined. C–G, n = 3 independent experiments; one-way analysis of variance (ANOVA) followed by Bonferroni's test (C–G). Data represent mean ± s.d. * p < 0.05, ** p < 0.01, *** p < 0.001.
    Figure Legend Snippet: ARID5A as a RNA-binding protein that stabilized IDO1 mRNAs via binding their 3′ UTRs. A, B, IDO1 expression was quantified in tissues from HCT116-ARID5A overexpression or tissues (A) from the HCT116-ARID5A KO group (B). C, RIP–qPCR analysis was performed with ARID5A antibody and IDO1 3′UTR-specific primers in HCT116-ARID5A vector or OE cell. D, IDO1 expression was quantified in cells from the HCT116-ARID5A overexpression or HCT116-ARID5A KO group. E, Luciferase analysis was performed on HCT116-ARID5A KO or OE cells. F, G, HCT116 or HT29 cells expressing ARID5A sgRNA or ARID5A OE at the indicated times in the presence of actinomycin D (1 μg ml −1 ). The remaining IDO1 mRNA levels were determined. C–G, n = 3 independent experiments; one-way analysis of variance (ANOVA) followed by Bonferroni's test (C–G). Data represent mean ± s.d. * p < 0.05, ** p < 0.01, *** p < 0.001.

    Techniques Used: RNA Binding Assay, Binding Assay, Expressing, Over Expression, Plasmid Preparation, Luciferase

    ht 29 htb 38  (ATCC)


    Bioz Verified Symbol ATCC is a verified supplier
    Bioz Manufacturer Symbol ATCC manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 86

    Structured Review

    ATCC ht 29 htb 38
    Impact of MM-129 (10 µM), IND (200 µM) or a combination of these agents (MM-129 + IND) on tumor development in DLD-1 ( a ), and <t>HT-29</t> ( b ) zebrafish xenografts, and the expression of human GAPDH mRNA was determined at 120 hpf. Data were presented as mean ± standard deviation (SD) and analyzed using one-way analysis of variance (ANOVA). n = 20 each group; ** p < 0.01, *** p < 0.001 vs. CON; ^ p < 0.05, ^^^ p < 0.001 vs. MM-129; # p < 0.05 vs. IND.
    Ht 29 Htb 38, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ht 29 htb 38/product/ATCC
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    ht 29 htb 38 - by Bioz Stars, 2024-05
    86/100 stars

    Images

    1) Product Images from "Assessment of an Anticancer Effect of the Simultaneous Administration of MM-129 and Indoximod in the Colorectal Cancer Model"

    Article Title: Assessment of an Anticancer Effect of the Simultaneous Administration of MM-129 and Indoximod in the Colorectal Cancer Model

    Journal: Cancers

    doi: 10.3390/cancers16010122

    Impact of MM-129 (10 µM), IND (200 µM) or a combination of these agents (MM-129 + IND) on tumor development in DLD-1 ( a ), and HT-29 ( b ) zebrafish xenografts, and the expression of human GAPDH mRNA was determined at 120 hpf. Data were presented as mean ± standard deviation (SD) and analyzed using one-way analysis of variance (ANOVA). n = 20 each group; ** p < 0.01, *** p < 0.001 vs. CON; ^ p < 0.05, ^^^ p < 0.001 vs. MM-129; # p < 0.05 vs. IND.
    Figure Legend Snippet: Impact of MM-129 (10 µM), IND (200 µM) or a combination of these agents (MM-129 + IND) on tumor development in DLD-1 ( a ), and HT-29 ( b ) zebrafish xenografts, and the expression of human GAPDH mRNA was determined at 120 hpf. Data were presented as mean ± standard deviation (SD) and analyzed using one-way analysis of variance (ANOVA). n = 20 each group; ** p < 0.01, *** p < 0.001 vs. CON; ^ p < 0.05, ^^^ p < 0.001 vs. MM-129; # p < 0.05 vs. IND.

    Techniques Used: Expressing, Standard Deviation

    Viability of DLD-1 ( a ) and HT-29 ( b ) colon cancer cells treated for 24 h with MM-129 (10 µM), IND (200 µM), and compound combination (MM-129 + IND). Results are presented as mean values ± standard deviation (SD) from three independent experiments performed in duplicate. * p < 0.05, ** p < 0.01, *** p < 0.001 vs. CON; ### p < 0.001 vs. IND.
    Figure Legend Snippet: Viability of DLD-1 ( a ) and HT-29 ( b ) colon cancer cells treated for 24 h with MM-129 (10 µM), IND (200 µM), and compound combination (MM-129 + IND). Results are presented as mean values ± standard deviation (SD) from three independent experiments performed in duplicate. * p < 0.05, ** p < 0.01, *** p < 0.001 vs. CON; ### p < 0.001 vs. IND.

    Techniques Used: Standard Deviation

    Representative dot-plots illustrating the loss of mitochondrial membrane potential, ΔΨm. ( a , b ) Flow cytometry dot-plots for the annexin V-FITC-propidium iodide assay ( c , d ) and quantitative chart illustrating the distribution of live, early, and late apoptotic and necrotic cells ( e , f ) of DLD-1 ( a , c , e ) and HT-29 ( b , d , f ) after 24 h exposition to MM-129 (10 µM), IND (200 µM), and MM-129 + IND. Values were obtained from three independent experiments.
    Figure Legend Snippet: Representative dot-plots illustrating the loss of mitochondrial membrane potential, ΔΨm. ( a , b ) Flow cytometry dot-plots for the annexin V-FITC-propidium iodide assay ( c , d ) and quantitative chart illustrating the distribution of live, early, and late apoptotic and necrotic cells ( e , f ) of DLD-1 ( a , c , e ) and HT-29 ( b , d , f ) after 24 h exposition to MM-129 (10 µM), IND (200 µM), and MM-129 + IND. Values were obtained from three independent experiments.

    Techniques Used: Membrane, Flow Cytometry

    Flow cytometric analysis of caspase-3/7, caspase-10, and caspase-8 activation in DLD-1 ( a – c ) and HT-29 ( d – f ) colon cancer cells exposed to MM-129 (10 µM), IND (200 µM), and MM-129 + IND for 24 h. Values from three independent experiments performed in duplicate were presented as mean ± standard deviation (SD) and analyzed using one-way analysis of variance (ANOVA). ** p < 0.01, *** p < 0.001 vs. CON; ^ p < 0.05, ^^ p < 0.01, ^^^ p < 0.001vs. MM-129; ### p < 0.001 vs. IND; && p < 0.01, &&& p < 0.001 IND vs. MM-129.
    Figure Legend Snippet: Flow cytometric analysis of caspase-3/7, caspase-10, and caspase-8 activation in DLD-1 ( a – c ) and HT-29 ( d – f ) colon cancer cells exposed to MM-129 (10 µM), IND (200 µM), and MM-129 + IND for 24 h. Values from three independent experiments performed in duplicate were presented as mean ± standard deviation (SD) and analyzed using one-way analysis of variance (ANOVA). ** p < 0.01, *** p < 0.001 vs. CON; ^ p < 0.05, ^^ p < 0.01, ^^^ p < 0.001vs. MM-129; ### p < 0.001 vs. IND; && p < 0.01, &&& p < 0.001 IND vs. MM-129.

    Techniques Used: Activation Assay, Standard Deviation

    The downregulation of protein kinase B (AKT) ( a , b ) and indoleamine 2,3-dioxygenase-1 (IDO1) ( c , d ) exerted by MM-129 (10 µM), IND (200 µM), or MM-129 + IND toward DLD-1 and HT-29 colorectal cancer cells after 24 h exposition. The values were obtained from three independent experiments performed in duplicate. * p < 0.05, *** p < 0.001 vs. CON; ^^^ p < 0.01 vs. MM-129; ## p < 0.01, ### p < 0.001 vs. IND. Images and quantification were obtained by capillary protein separation and immunodetection. The uncropped blots are shown in the .
    Figure Legend Snippet: The downregulation of protein kinase B (AKT) ( a , b ) and indoleamine 2,3-dioxygenase-1 (IDO1) ( c , d ) exerted by MM-129 (10 µM), IND (200 µM), or MM-129 + IND toward DLD-1 and HT-29 colorectal cancer cells after 24 h exposition. The values were obtained from three independent experiments performed in duplicate. * p < 0.05, *** p < 0.001 vs. CON; ^^^ p < 0.01 vs. MM-129; ## p < 0.01, ### p < 0.001 vs. IND. Images and quantification were obtained by capillary protein separation and immunodetection. The uncropped blots are shown in the .

    Techniques Used: Immunodetection

    Similar Products

  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 86
    ATCC atcc htb 38 ovcar
    Atcc Htb 38 Ovcar, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/atcc htb 38 ovcar/product/ATCC
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    atcc htb 38 ovcar - by Bioz Stars, 2024-05
    86/100 stars
      Buy from Supplier

    86
    ATCC ht 29 htb 38 cells
    Biodistribution of [ 89 Zr]-DFO(0.7)-RW03 IgG , [ 89 Zr]-DFO(3.0)-RW03 IgG , [ 89 Zr]-DFO(2.9)-RW03 scFv-Fc in <t>HT-29</t> xenograft BALB/c nu/nu mice. ( a ) [ 89 Zr]-DFO(0.7)-RW03 IgG (0.16 ± 0.02 MBq, 5 ± 0.7 ug) at 24, 48, 96 h p.i., ( b ) [ 89 Zr]-DFO(3.0)-RW03 IgG (0.14 ± 0.03 MBq, 1 ± 0.3 µg) at 24, 48, 96 h p.i., ( c ) [ 89 Zr]-DFO(2.9)-RW03 scFv-Fc (0.085 ± 0.02 MBq, 1 ± 0.2 µg) at 24, 48, and 72 h p.i. The blocked cohort received [ 89 Zr]-DFO-RW03 scFv-Fc (0.09 ± 0.02 MBq, 1.0 ± 0.2 µg) and excess RW03 IgG (400 µg). Uptake is expressed as mean percentage injected dose per gram tissue (%ID/g) ± SEM.
    Ht 29 Htb 38 Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ht 29 htb 38 cells/product/ATCC
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    ht 29 htb 38 cells - by Bioz Stars, 2024-05
    86/100 stars
      Buy from Supplier

    htb 38  (ATCC)
    86
    ATCC htb 38
    Biodistribution of [ 89 Zr]-DFO(0.7)-RW03 IgG , [ 89 Zr]-DFO(3.0)-RW03 IgG , [ 89 Zr]-DFO(2.9)-RW03 scFv-Fc in <t>HT-29</t> xenograft BALB/c nu/nu mice. ( a ) [ 89 Zr]-DFO(0.7)-RW03 IgG (0.16 ± 0.02 MBq, 5 ± 0.7 ug) at 24, 48, 96 h p.i., ( b ) [ 89 Zr]-DFO(3.0)-RW03 IgG (0.14 ± 0.03 MBq, 1 ± 0.3 µg) at 24, 48, 96 h p.i., ( c ) [ 89 Zr]-DFO(2.9)-RW03 scFv-Fc (0.085 ± 0.02 MBq, 1 ± 0.2 µg) at 24, 48, and 72 h p.i. The blocked cohort received [ 89 Zr]-DFO-RW03 scFv-Fc (0.09 ± 0.02 MBq, 1.0 ± 0.2 µg) and excess RW03 IgG (400 µg). Uptake is expressed as mean percentage injected dose per gram tissue (%ID/g) ± SEM.
    Htb 38, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/htb 38/product/ATCC
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    htb 38 - by Bioz Stars, 2024-05
    86/100 stars
      Buy from Supplier

    86
    ATCC cell line ht 29 htb 38
    Biodistribution of [ 89 Zr]-DFO(0.7)-RW03 IgG , [ 89 Zr]-DFO(3.0)-RW03 IgG , [ 89 Zr]-DFO(2.9)-RW03 scFv-Fc in <t>HT-29</t> xenograft BALB/c nu/nu mice. ( a ) [ 89 Zr]-DFO(0.7)-RW03 IgG (0.16 ± 0.02 MBq, 5 ± 0.7 ug) at 24, 48, 96 h p.i., ( b ) [ 89 Zr]-DFO(3.0)-RW03 IgG (0.14 ± 0.03 MBq, 1 ± 0.3 µg) at 24, 48, 96 h p.i., ( c ) [ 89 Zr]-DFO(2.9)-RW03 scFv-Fc (0.085 ± 0.02 MBq, 1 ± 0.2 µg) at 24, 48, and 72 h p.i. The blocked cohort received [ 89 Zr]-DFO-RW03 scFv-Fc (0.09 ± 0.02 MBq, 1.0 ± 0.2 µg) and excess RW03 IgG (400 µg). Uptake is expressed as mean percentage injected dose per gram tissue (%ID/g) ± SEM.
    Cell Line Ht 29 Htb 38, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/cell line ht 29 htb 38/product/ATCC
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    cell line ht 29 htb 38 - by Bioz Stars, 2024-05
    86/100 stars
      Buy from Supplier

    86
    ATCC ht29 htb 38 cell lines
    ARID5A as a RNA-binding protein that stabilized IDO1 mRNAs via binding their 3′ UTRs. A, B, IDO1 expression was quantified in tissues from HCT116-ARID5A overexpression or tissues (A) from the HCT116-ARID5A KO group (B). C, RIP–qPCR analysis was performed with ARID5A antibody and IDO1 3′UTR-specific primers in HCT116-ARID5A vector or OE cell. D, IDO1 expression was quantified in cells from the HCT116-ARID5A overexpression or HCT116-ARID5A KO group. E, Luciferase analysis was performed on HCT116-ARID5A KO or OE cells. F, G, HCT116 or <t>HT29</t> cells expressing ARID5A sgRNA or ARID5A OE at the indicated times in the presence of actinomycin D (1 μg ml −1 ). The remaining IDO1 mRNA levels were determined. C–G, n = 3 independent experiments; one-way analysis of variance (ANOVA) followed by Bonferroni's test (C–G). Data represent mean ± s.d. * p < 0.05, ** p < 0.01, *** p < 0.001.
    Ht29 Htb 38 Cell Lines, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ht29 htb 38 cell lines/product/ATCC
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    ht29 htb 38 cell lines - by Bioz Stars, 2024-05
    86/100 stars
      Buy from Supplier

    86
    ATCC ht 29 htb 38
    Impact of MM-129 (10 µM), IND (200 µM) or a combination of these agents (MM-129 + IND) on tumor development in DLD-1 ( a ), and <t>HT-29</t> ( b ) zebrafish xenografts, and the expression of human GAPDH mRNA was determined at 120 hpf. Data were presented as mean ± standard deviation (SD) and analyzed using one-way analysis of variance (ANOVA). n = 20 each group; ** p < 0.01, *** p < 0.001 vs. CON; ^ p < 0.05, ^^^ p < 0.001 vs. MM-129; # p < 0.05 vs. IND.
    Ht 29 Htb 38, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ht 29 htb 38/product/ATCC
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    ht 29 htb 38 - by Bioz Stars, 2024-05
    86/100 stars
      Buy from Supplier

    Image Search Results


    Biodistribution of [ 89 Zr]-DFO(0.7)-RW03 IgG , [ 89 Zr]-DFO(3.0)-RW03 IgG , [ 89 Zr]-DFO(2.9)-RW03 scFv-Fc in HT-29 xenograft BALB/c nu/nu mice. ( a ) [ 89 Zr]-DFO(0.7)-RW03 IgG (0.16 ± 0.02 MBq, 5 ± 0.7 ug) at 24, 48, 96 h p.i., ( b ) [ 89 Zr]-DFO(3.0)-RW03 IgG (0.14 ± 0.03 MBq, 1 ± 0.3 µg) at 24, 48, 96 h p.i., ( c ) [ 89 Zr]-DFO(2.9)-RW03 scFv-Fc (0.085 ± 0.02 MBq, 1 ± 0.2 µg) at 24, 48, and 72 h p.i. The blocked cohort received [ 89 Zr]-DFO-RW03 scFv-Fc (0.09 ± 0.02 MBq, 1.0 ± 0.2 µg) and excess RW03 IgG (400 µg). Uptake is expressed as mean percentage injected dose per gram tissue (%ID/g) ± SEM.

    Journal: EJNMMI Research

    Article Title: 89 Zr-labeled ImmunoPET targeting the cancer stem cell antigen CD133 using fully-human antibody constructs

    doi: 10.1186/s13550-024-01091-9

    Figure Lengend Snippet: Biodistribution of [ 89 Zr]-DFO(0.7)-RW03 IgG , [ 89 Zr]-DFO(3.0)-RW03 IgG , [ 89 Zr]-DFO(2.9)-RW03 scFv-Fc in HT-29 xenograft BALB/c nu/nu mice. ( a ) [ 89 Zr]-DFO(0.7)-RW03 IgG (0.16 ± 0.02 MBq, 5 ± 0.7 ug) at 24, 48, 96 h p.i., ( b ) [ 89 Zr]-DFO(3.0)-RW03 IgG (0.14 ± 0.03 MBq, 1 ± 0.3 µg) at 24, 48, 96 h p.i., ( c ) [ 89 Zr]-DFO(2.9)-RW03 scFv-Fc (0.085 ± 0.02 MBq, 1 ± 0.2 µg) at 24, 48, and 72 h p.i. The blocked cohort received [ 89 Zr]-DFO-RW03 scFv-Fc (0.09 ± 0.02 MBq, 1.0 ± 0.2 µg) and excess RW03 IgG (400 µg). Uptake is expressed as mean percentage injected dose per gram tissue (%ID/g) ± SEM.

    Article Snippet: HT-29 (HTB-38™) cells were purchased from ATCC and grown in McCoy’s 5 A medium (ATCC 30-2007™) supplemented with 10% FBS, penicillin (100 IU) and streptomycin (100 µg).

    Techniques: Injection

    In vivo PET images ( a,c,e ) and corresponding ROI analysis (b, d, f) of [ 89 Zr]-RW03 based radioligands: ( a & b ) [ 89 Zr]-DFO(0.7)-RW03 IgG (1.27 ± 0.03 MBq, 10 ± 0.2 ug), ( c & d ) [ 89 Zr]-DFO(3.0)-RW03 IgG (5.2 ± 0.4 MBq, 27 ± 2 µg), ( e & f ) [ 89 Zr]-DFO(2.9)-RW03 scFv − Fc (1.2 ± 0.1 MBq, 16 ± 2 µg) in HT-29 xenograft BALB/c nu/nu mice. For PET images ( a, c, e ), the coronal plane is presented as a maximum intensity projection (MIP). White arrow = tumor. For ROI analysis ( b, d, f ) uptake is expressed as mean radioactivity per mL tissue volume (%ID/cc) ± SEM

    Journal: EJNMMI Research

    Article Title: 89 Zr-labeled ImmunoPET targeting the cancer stem cell antigen CD133 using fully-human antibody constructs

    doi: 10.1186/s13550-024-01091-9

    Figure Lengend Snippet: In vivo PET images ( a,c,e ) and corresponding ROI analysis (b, d, f) of [ 89 Zr]-RW03 based radioligands: ( a & b ) [ 89 Zr]-DFO(0.7)-RW03 IgG (1.27 ± 0.03 MBq, 10 ± 0.2 ug), ( c & d ) [ 89 Zr]-DFO(3.0)-RW03 IgG (5.2 ± 0.4 MBq, 27 ± 2 µg), ( e & f ) [ 89 Zr]-DFO(2.9)-RW03 scFv − Fc (1.2 ± 0.1 MBq, 16 ± 2 µg) in HT-29 xenograft BALB/c nu/nu mice. For PET images ( a, c, e ), the coronal plane is presented as a maximum intensity projection (MIP). White arrow = tumor. For ROI analysis ( b, d, f ) uptake is expressed as mean radioactivity per mL tissue volume (%ID/cc) ± SEM

    Article Snippet: HT-29 (HTB-38™) cells were purchased from ATCC and grown in McCoy’s 5 A medium (ATCC 30-2007™) supplemented with 10% FBS, penicillin (100 IU) and streptomycin (100 µg).

    Techniques: In Vivo, Radioactivity

    ARID5A as a RNA-binding protein that stabilized IDO1 mRNAs via binding their 3′ UTRs. A, B, IDO1 expression was quantified in tissues from HCT116-ARID5A overexpression or tissues (A) from the HCT116-ARID5A KO group (B). C, RIP–qPCR analysis was performed with ARID5A antibody and IDO1 3′UTR-specific primers in HCT116-ARID5A vector or OE cell. D, IDO1 expression was quantified in cells from the HCT116-ARID5A overexpression or HCT116-ARID5A KO group. E, Luciferase analysis was performed on HCT116-ARID5A KO or OE cells. F, G, HCT116 or HT29 cells expressing ARID5A sgRNA or ARID5A OE at the indicated times in the presence of actinomycin D (1 μg ml −1 ). The remaining IDO1 mRNA levels were determined. C–G, n = 3 independent experiments; one-way analysis of variance (ANOVA) followed by Bonferroni's test (C–G). Data represent mean ± s.d. * p < 0.05, ** p < 0.01, *** p < 0.001.

    Journal: Translational Oncology

    Article Title: ARID5A stabilizes Indoleamine 2,3-dioxygenase expression and enhances CAR T cell exhaustion in colorectal cancer

    doi: 10.1016/j.tranon.2024.101900

    Figure Lengend Snippet: ARID5A as a RNA-binding protein that stabilized IDO1 mRNAs via binding their 3′ UTRs. A, B, IDO1 expression was quantified in tissues from HCT116-ARID5A overexpression or tissues (A) from the HCT116-ARID5A KO group (B). C, RIP–qPCR analysis was performed with ARID5A antibody and IDO1 3′UTR-specific primers in HCT116-ARID5A vector or OE cell. D, IDO1 expression was quantified in cells from the HCT116-ARID5A overexpression or HCT116-ARID5A KO group. E, Luciferase analysis was performed on HCT116-ARID5A KO or OE cells. F, G, HCT116 or HT29 cells expressing ARID5A sgRNA or ARID5A OE at the indicated times in the presence of actinomycin D (1 μg ml −1 ). The remaining IDO1 mRNA levels were determined. C–G, n = 3 independent experiments; one-way analysis of variance (ANOVA) followed by Bonferroni's test (C–G). Data represent mean ± s.d. * p < 0.05, ** p < 0.01, *** p < 0.001.

    Article Snippet: HCT116 cell line (CCL-247) and HT29 (HTB-38) cell lines were obtained from the American Type Culture Collection, United States.

    Techniques: RNA Binding Assay, Binding Assay, Expressing, Over Expression, Plasmid Preparation, Luciferase

    Impact of MM-129 (10 µM), IND (200 µM) or a combination of these agents (MM-129 + IND) on tumor development in DLD-1 ( a ), and HT-29 ( b ) zebrafish xenografts, and the expression of human GAPDH mRNA was determined at 120 hpf. Data were presented as mean ± standard deviation (SD) and analyzed using one-way analysis of variance (ANOVA). n = 20 each group; ** p < 0.01, *** p < 0.001 vs. CON; ^ p < 0.05, ^^^ p < 0.001 vs. MM-129; # p < 0.05 vs. IND.

    Journal: Cancers

    Article Title: Assessment of an Anticancer Effect of the Simultaneous Administration of MM-129 and Indoximod in the Colorectal Cancer Model

    doi: 10.3390/cancers16010122

    Figure Lengend Snippet: Impact of MM-129 (10 µM), IND (200 µM) or a combination of these agents (MM-129 + IND) on tumor development in DLD-1 ( a ), and HT-29 ( b ) zebrafish xenografts, and the expression of human GAPDH mRNA was determined at 120 hpf. Data were presented as mean ± standard deviation (SD) and analyzed using one-way analysis of variance (ANOVA). n = 20 each group; ** p < 0.01, *** p < 0.001 vs. CON; ^ p < 0.05, ^^^ p < 0.001 vs. MM-129; # p < 0.05 vs. IND.

    Article Snippet: Two human colorectal adenocarcinoma cell lines DLD-1 (CCL-221) and HT-29 (HTB-38) were purchased from the American Type Culture Collection (ATCC, Manassas, VA, USA).

    Techniques: Expressing, Standard Deviation

    Viability of DLD-1 ( a ) and HT-29 ( b ) colon cancer cells treated for 24 h with MM-129 (10 µM), IND (200 µM), and compound combination (MM-129 + IND). Results are presented as mean values ± standard deviation (SD) from three independent experiments performed in duplicate. * p < 0.05, ** p < 0.01, *** p < 0.001 vs. CON; ### p < 0.001 vs. IND.

    Journal: Cancers

    Article Title: Assessment of an Anticancer Effect of the Simultaneous Administration of MM-129 and Indoximod in the Colorectal Cancer Model

    doi: 10.3390/cancers16010122

    Figure Lengend Snippet: Viability of DLD-1 ( a ) and HT-29 ( b ) colon cancer cells treated for 24 h with MM-129 (10 µM), IND (200 µM), and compound combination (MM-129 + IND). Results are presented as mean values ± standard deviation (SD) from three independent experiments performed in duplicate. * p < 0.05, ** p < 0.01, *** p < 0.001 vs. CON; ### p < 0.001 vs. IND.

    Article Snippet: Two human colorectal adenocarcinoma cell lines DLD-1 (CCL-221) and HT-29 (HTB-38) were purchased from the American Type Culture Collection (ATCC, Manassas, VA, USA).

    Techniques: Standard Deviation

    Representative dot-plots illustrating the loss of mitochondrial membrane potential, ΔΨm. ( a , b ) Flow cytometry dot-plots for the annexin V-FITC-propidium iodide assay ( c , d ) and quantitative chart illustrating the distribution of live, early, and late apoptotic and necrotic cells ( e , f ) of DLD-1 ( a , c , e ) and HT-29 ( b , d , f ) after 24 h exposition to MM-129 (10 µM), IND (200 µM), and MM-129 + IND. Values were obtained from three independent experiments.

    Journal: Cancers

    Article Title: Assessment of an Anticancer Effect of the Simultaneous Administration of MM-129 and Indoximod in the Colorectal Cancer Model

    doi: 10.3390/cancers16010122

    Figure Lengend Snippet: Representative dot-plots illustrating the loss of mitochondrial membrane potential, ΔΨm. ( a , b ) Flow cytometry dot-plots for the annexin V-FITC-propidium iodide assay ( c , d ) and quantitative chart illustrating the distribution of live, early, and late apoptotic and necrotic cells ( e , f ) of DLD-1 ( a , c , e ) and HT-29 ( b , d , f ) after 24 h exposition to MM-129 (10 µM), IND (200 µM), and MM-129 + IND. Values were obtained from three independent experiments.

    Article Snippet: Two human colorectal adenocarcinoma cell lines DLD-1 (CCL-221) and HT-29 (HTB-38) were purchased from the American Type Culture Collection (ATCC, Manassas, VA, USA).

    Techniques: Membrane, Flow Cytometry

    Flow cytometric analysis of caspase-3/7, caspase-10, and caspase-8 activation in DLD-1 ( a – c ) and HT-29 ( d – f ) colon cancer cells exposed to MM-129 (10 µM), IND (200 µM), and MM-129 + IND for 24 h. Values from three independent experiments performed in duplicate were presented as mean ± standard deviation (SD) and analyzed using one-way analysis of variance (ANOVA). ** p < 0.01, *** p < 0.001 vs. CON; ^ p < 0.05, ^^ p < 0.01, ^^^ p < 0.001vs. MM-129; ### p < 0.001 vs. IND; && p < 0.01, &&& p < 0.001 IND vs. MM-129.

    Journal: Cancers

    Article Title: Assessment of an Anticancer Effect of the Simultaneous Administration of MM-129 and Indoximod in the Colorectal Cancer Model

    doi: 10.3390/cancers16010122

    Figure Lengend Snippet: Flow cytometric analysis of caspase-3/7, caspase-10, and caspase-8 activation in DLD-1 ( a – c ) and HT-29 ( d – f ) colon cancer cells exposed to MM-129 (10 µM), IND (200 µM), and MM-129 + IND for 24 h. Values from three independent experiments performed in duplicate were presented as mean ± standard deviation (SD) and analyzed using one-way analysis of variance (ANOVA). ** p < 0.01, *** p < 0.001 vs. CON; ^ p < 0.05, ^^ p < 0.01, ^^^ p < 0.001vs. MM-129; ### p < 0.001 vs. IND; && p < 0.01, &&& p < 0.001 IND vs. MM-129.

    Article Snippet: Two human colorectal adenocarcinoma cell lines DLD-1 (CCL-221) and HT-29 (HTB-38) were purchased from the American Type Culture Collection (ATCC, Manassas, VA, USA).

    Techniques: Activation Assay, Standard Deviation

    The downregulation of protein kinase B (AKT) ( a , b ) and indoleamine 2,3-dioxygenase-1 (IDO1) ( c , d ) exerted by MM-129 (10 µM), IND (200 µM), or MM-129 + IND toward DLD-1 and HT-29 colorectal cancer cells after 24 h exposition. The values were obtained from three independent experiments performed in duplicate. * p < 0.05, *** p < 0.001 vs. CON; ^^^ p < 0.01 vs. MM-129; ## p < 0.01, ### p < 0.001 vs. IND. Images and quantification were obtained by capillary protein separation and immunodetection. The uncropped blots are shown in the .

    Journal: Cancers

    Article Title: Assessment of an Anticancer Effect of the Simultaneous Administration of MM-129 and Indoximod in the Colorectal Cancer Model

    doi: 10.3390/cancers16010122

    Figure Lengend Snippet: The downregulation of protein kinase B (AKT) ( a , b ) and indoleamine 2,3-dioxygenase-1 (IDO1) ( c , d ) exerted by MM-129 (10 µM), IND (200 µM), or MM-129 + IND toward DLD-1 and HT-29 colorectal cancer cells after 24 h exposition. The values were obtained from three independent experiments performed in duplicate. * p < 0.05, *** p < 0.001 vs. CON; ^^^ p < 0.01 vs. MM-129; ## p < 0.01, ### p < 0.001 vs. IND. Images and quantification were obtained by capillary protein separation and immunodetection. The uncropped blots are shown in the .

    Article Snippet: Two human colorectal adenocarcinoma cell lines DLD-1 (CCL-221) and HT-29 (HTB-38) were purchased from the American Type Culture Collection (ATCC, Manassas, VA, USA).

    Techniques: Immunodetection