h209  (ATCC)


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    ATCC h209
    H209, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    a 172 crl 1620 glioblastoma 1 067 a 204 htb 82 rhabdomyosarcoma 1 360 a375 crl  (ATCC)


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    ATCC a 172 crl 1620 glioblastoma 1 067 a 204 htb 82 rhabdomyosarcoma 1 360 a375 crl
    A 172 Crl 1620 Glioblastoma 1 067 A 204 Htb 82 Rhabdomyosarcoma 1 360 A375 Crl, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    htb 172 h2170 atcc  (ATCC)


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    ATCC htb 172 h2170 atcc
    Htb 172 H2170 Atcc, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    h209  (ATCC)


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    ATCC h209
    H209, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    nci h209  (ATCC)


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    ATCC nci h209
    Expression of GIRK1 in some SCLC cell lines but not in normal SAE cells, nor in adenocarcinoma Clara cell phenotype cell lines . Top panel: GIRK1 is expressed in WBA, H69, and H146 SCLC cell lines but not in H187, <t>H209</t> and H526 SCLC cell lines. GIRK1 is also not expressed in normal SAE cells, nor is it expressed in H322 or H441 adenocarcinoma Clara cell lines. Bottom panel: Cyclophilin, used as a positive reaction control was seen in all samples. For all gene expression experiments, negative control reactions were performed and found to be negative. The bands on the agarose gels were consistent with the expected sizes: GIRK1-441 bp; cyclophilin-216 bp; M-100 bp.
    Nci H209, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    1) Product Images from "Expression of G-protein inwardly rectifying potassium channels (GIRKs) in lung cancer cell lines"

    Article Title: Expression of G-protein inwardly rectifying potassium channels (GIRKs) in lung cancer cell lines

    Journal: BMC Cancer

    doi: 10.1186/1471-2407-5-104

    Expression of GIRK1 in some SCLC cell lines but not in normal SAE cells, nor in adenocarcinoma Clara cell phenotype cell lines . Top panel: GIRK1 is expressed in WBA, H69, and H146 SCLC cell lines but not in H187, H209 and H526 SCLC cell lines. GIRK1 is also not expressed in normal SAE cells, nor is it expressed in H322 or H441 adenocarcinoma Clara cell lines. Bottom panel: Cyclophilin, used as a positive reaction control was seen in all samples. For all gene expression experiments, negative control reactions were performed and found to be negative. The bands on the agarose gels were consistent with the expected sizes: GIRK1-441 bp; cyclophilin-216 bp; M-100 bp.
    Figure Legend Snippet: Expression of GIRK1 in some SCLC cell lines but not in normal SAE cells, nor in adenocarcinoma Clara cell phenotype cell lines . Top panel: GIRK1 is expressed in WBA, H69, and H146 SCLC cell lines but not in H187, H209 and H526 SCLC cell lines. GIRK1 is also not expressed in normal SAE cells, nor is it expressed in H322 or H441 adenocarcinoma Clara cell lines. Bottom panel: Cyclophilin, used as a positive reaction control was seen in all samples. For all gene expression experiments, negative control reactions were performed and found to be negative. The bands on the agarose gels were consistent with the expected sizes: GIRK1-441 bp; cyclophilin-216 bp; M-100 bp.

    Techniques Used: Expressing, Negative Control

    Expression of GIRK3 in SCLC cell lines . Top panel: GIRK3 expression was seen in all six SCLC cell lines, in WBA, H69, H146, H187, H209, and H526. Bottom panel: Cyclophilin, used as a positive reaction control was seen in all samples. For all gene expression experiments, negative control reactions were performed and found to be negative. The bands on the agarose gels were consistent with the expected sizes: GIRK3-317 bp; cyclophilin-216 bp; M-100 bp.
    Figure Legend Snippet: Expression of GIRK3 in SCLC cell lines . Top panel: GIRK3 expression was seen in all six SCLC cell lines, in WBA, H69, H146, H187, H209, and H526. Bottom panel: Cyclophilin, used as a positive reaction control was seen in all samples. For all gene expression experiments, negative control reactions were performed and found to be negative. The bands on the agarose gels were consistent with the expected sizes: GIRK3-317 bp; cyclophilin-216 bp; M-100 bp.

    Techniques Used: Expressing, Negative Control

    Summary of GIRK mRNA expression in lung cells. This is a compilation of data shown in Figures 1–6 & Table 1. Some of the indicated data was not shown.
    Figure Legend Snippet: Summary of GIRK mRNA expression in lung cells. This is a compilation of data shown in Figures 1–6 & Table 1. Some of the indicated data was not shown.

    Techniques Used: Expressing

    Expression of GIRK2 or GIRK4 in small cell lung cancer cell lines. Expression of either GIRK2 or GIRK4 was determined by real time PCR. Threshold values (C T ) values are listed below.
    Figure Legend Snippet: Expression of GIRK2 or GIRK4 in small cell lung cancer cell lines. Expression of either GIRK2 or GIRK4 was determined by real time PCR. Threshold values (C T ) values are listed below.

    Techniques Used: Expressing, Real-time Polymerase Chain Reaction

    nci h209  (ATCC)


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    ATCC nci h209
    Comparison of lentivirus transduction efficiency with AAVs in the AAV-resistant human lung cancer cell lines. <t>NCI-H510,</t> NCI-H82, NCI-H69, <t>NCI-H209</t> and NCI-H727 cells were infected with A ) MOI 100 AAV2/1, AAV2/2 and AAV2/5 and compared to with B ) lentivirus at increasing MOI 0.04-125. Except for NCI-H209 cells, the other lung cancer cell lines that were refractory to rAAVs viral transduction were susceptible to the lentivirus infection. The infection was done in triplicate on two separate occasions. Data are presented as the mean of positive cells ± SEM.
    Nci H209, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    1) Product Images from "Comparative analysis of the transduction efficiency of five adeno associated virus serotypes and VSV-G pseudotype lentiviral vector in lung cancer cells"

    Article Title: Comparative analysis of the transduction efficiency of five adeno associated virus serotypes and VSV-G pseudotype lentiviral vector in lung cancer cells

    Journal: Virology Journal

    doi: 10.1186/1743-422X-10-86

    Comparison of lentivirus transduction efficiency with AAVs in the AAV-resistant human lung cancer cell lines. NCI-H510, NCI-H82, NCI-H69, NCI-H209 and NCI-H727 cells were infected with A ) MOI 100 AAV2/1, AAV2/2 and AAV2/5 and compared to with B ) lentivirus at increasing MOI 0.04-125. Except for NCI-H209 cells, the other lung cancer cell lines that were refractory to rAAVs viral transduction were susceptible to the lentivirus infection. The infection was done in triplicate on two separate occasions. Data are presented as the mean of positive cells ± SEM.
    Figure Legend Snippet: Comparison of lentivirus transduction efficiency with AAVs in the AAV-resistant human lung cancer cell lines. NCI-H510, NCI-H82, NCI-H69, NCI-H209 and NCI-H727 cells were infected with A ) MOI 100 AAV2/1, AAV2/2 and AAV2/5 and compared to with B ) lentivirus at increasing MOI 0.04-125. Except for NCI-H209 cells, the other lung cancer cell lines that were refractory to rAAVs viral transduction were susceptible to the lentivirus infection. The infection was done in triplicate on two separate occasions. Data are presented as the mean of positive cells ± SEM.

    Techniques Used: Transduction, Infection

    Infection rates of 5 serotypes of AAV in 11 lung cancer cell lines
    Figure Legend Snippet: Infection rates of 5 serotypes of AAV in 11 lung cancer cell lines

    Techniques Used: Infection

    nci h209  (ATCC)


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    ATCC nci h209
    Detection of Derlin-1 expression in a panel of lung cancer cells by western blot. Derlin-1 expression was detected in all cell lines. Derlin-1 expression was high in 95-D, A549, Calu-3, GLC-82, <t>NCI-N460</t> and LTEP-a-2, but low in <t>NCI-H209</t> and NCI-H446.
    Nci H209, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    1) Product Images from "Expression of Derlin-1 and its effect on expression of autophagy marker genes under endoplasmic reticulum stress in lung cancer cells"

    Article Title: Expression of Derlin-1 and its effect on expression of autophagy marker genes under endoplasmic reticulum stress in lung cancer cells

    Journal: Cancer Cell International

    doi: 10.1186/1475-2867-14-50

    Detection of Derlin-1 expression in a panel of lung cancer cells by western blot. Derlin-1 expression was detected in all cell lines. Derlin-1 expression was high in 95-D, A549, Calu-3, GLC-82, NCI-N460 and LTEP-a-2, but low in NCI-H209 and NCI-H446.
    Figure Legend Snippet: Detection of Derlin-1 expression in a panel of lung cancer cells by western blot. Derlin-1 expression was detected in all cell lines. Derlin-1 expression was high in 95-D, A549, Calu-3, GLC-82, NCI-N460 and LTEP-a-2, but low in NCI-H209 and NCI-H446.

    Techniques Used: Expressing, Western Blot

    nci h209  (ATCC)


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    ATCC nci h209
    Nci H209, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    nci h209  (ATCC)


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    ATCC nci h209
    Agarose gel showing α7 nicotinic acetylcholine receptor expression in SCLC cell lines. cDNA was amplified by PCR using the human α7 primers. SCLC cell lines: 1 ) WBA; 2 ) <t>NCI-H69;</t> 3 ) NCI-H82; 4 ) NCI-H146; 5 ) NCI-H187; 6 ) <t>NCI-H209;</t> 7 ) NCI-H526. For all gene expression experiments, negative control reactions were performed and found to be negative. The bands were consistent with the expected size, 335 bp. M-100 bp DNA ladder.
    Nci H209, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    1) Product Images from "Expression of the α7 nicotinic acetylcholine receptor in human lung cells"

    Article Title: Expression of the α7 nicotinic acetylcholine receptor in human lung cells

    Journal: Respiratory Research

    doi: 10.1186/1465-9921-6-29

    Agarose gel showing α7 nicotinic acetylcholine receptor expression in SCLC cell lines. cDNA was amplified by PCR using the human α7 primers. SCLC cell lines: 1 ) WBA; 2 ) NCI-H69; 3 ) NCI-H82; 4 ) NCI-H146; 5 ) NCI-H187; 6 ) NCI-H209; 7 ) NCI-H526. For all gene expression experiments, negative control reactions were performed and found to be negative. The bands were consistent with the expected size, 335 bp. M-100 bp DNA ladder.
    Figure Legend Snippet: Agarose gel showing α7 nicotinic acetylcholine receptor expression in SCLC cell lines. cDNA was amplified by PCR using the human α7 primers. SCLC cell lines: 1 ) WBA; 2 ) NCI-H69; 3 ) NCI-H82; 4 ) NCI-H146; 5 ) NCI-H187; 6 ) NCI-H209; 7 ) NCI-H526. For all gene expression experiments, negative control reactions were performed and found to be negative. The bands were consistent with the expected size, 335 bp. M-100 bp DNA ladder.

    Techniques Used: Agarose Gel Electrophoresis, Expressing, Amplification, Negative Control

    Expression of α7 nicotinic acetylcholine receptor protein in SCLC cell lines as assessed by western blot analysis. Protein was isolated with the ReadyPrep protein extraction kit (signal) (Biorad). Nitrocellulose membranes were incubated with the rabbit polyclonal antibody to the alpha 7 nicotinic acetylcholine receptor (Abcam). 1 ) WBA; 2 ) NCI-H69; 3 ) NCI-H82; 4 ) NCI-H146; 5 ) NCI-H187; 6 ) NCI-H209; 7 ) NCI-H526. The arrow indicates the 56 kDa band (expected size). All SCLC cell lines except NCI-H187 express protein for alpha 7 nicotinic acetylcholine receptor.
    Figure Legend Snippet: Expression of α7 nicotinic acetylcholine receptor protein in SCLC cell lines as assessed by western blot analysis. Protein was isolated with the ReadyPrep protein extraction kit (signal) (Biorad). Nitrocellulose membranes were incubated with the rabbit polyclonal antibody to the alpha 7 nicotinic acetylcholine receptor (Abcam). 1 ) WBA; 2 ) NCI-H69; 3 ) NCI-H82; 4 ) NCI-H146; 5 ) NCI-H187; 6 ) NCI-H209; 7 ) NCI-H526. The arrow indicates the 56 kDa band (expected size). All SCLC cell lines except NCI-H187 express protein for alpha 7 nicotinic acetylcholine receptor.

    Techniques Used: Expressing, Western Blot, Isolation, Protein Extraction, Incubation

    nci h209 cell line  (ATCC)


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    ATCC nci h209 cell line
    (A) Circos plot representing copy number variations, inter and intra-chromosomal rearrangements of <t>NCI-H209</t> cell line and a xenograft tumour derived from it. Copy number variations (red, gain; green, loss) were calculated based on coverage using the correspondent peripheral blood as control. Inter and intra-chromosomal rearrangements are represented in blue (inter-chromosomal) and dark blue (intra-chromosomal). (B, C) Detailed profile of copy number variations and B-allele frequencies of chromosome 1 from the analysed cell line and xenograft. As described above, the correspondent peripheral blood was used as control for both type of analysis. Copy number profiles are shown in red (gain), green (loss) and grey (no change). LOH are shown light blue.
    Nci H209 Cell Line, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    1) Product Images from "Next-Generation Sequence Analysis of Cancer Xenograft Models"

    Article Title: Next-Generation Sequence Analysis of Cancer Xenograft Models

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0074432

    (A) Circos plot representing copy number variations, inter and intra-chromosomal rearrangements of NCI-H209 cell line and a xenograft tumour derived from it. Copy number variations (red, gain; green, loss) were calculated based on coverage using the correspondent peripheral blood as control. Inter and intra-chromosomal rearrangements are represented in blue (inter-chromosomal) and dark blue (intra-chromosomal). (B, C) Detailed profile of copy number variations and B-allele frequencies of chromosome 1 from the analysed cell line and xenograft. As described above, the correspondent peripheral blood was used as control for both type of analysis. Copy number profiles are shown in red (gain), green (loss) and grey (no change). LOH are shown light blue.
    Figure Legend Snippet: (A) Circos plot representing copy number variations, inter and intra-chromosomal rearrangements of NCI-H209 cell line and a xenograft tumour derived from it. Copy number variations (red, gain; green, loss) were calculated based on coverage using the correspondent peripheral blood as control. Inter and intra-chromosomal rearrangements are represented in blue (inter-chromosomal) and dark blue (intra-chromosomal). (B, C) Detailed profile of copy number variations and B-allele frequencies of chromosome 1 from the analysed cell line and xenograft. As described above, the correspondent peripheral blood was used as control for both type of analysis. Copy number profiles are shown in red (gain), green (loss) and grey (no change). LOH are shown light blue.

    Techniques Used: Derivative Assay

    h209  (ATCC)


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    ATCC h209

    H209, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    1) Product Images from "MAPK pathway activation selectively inhibits ASCL1-driven small cell lung cancer"

    Article Title: MAPK pathway activation selectively inhibits ASCL1-driven small cell lung cancer

    Journal: iScience

    doi: 10.1016/j.isci.2021.103224


    Figure Legend Snippet:

    Techniques Used: Recombinant, Flow Cytometry, Software

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    ATCC h209
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    ATCC a 172 crl 1620 glioblastoma 1 067 a 204 htb 82 rhabdomyosarcoma 1 360 a375 crl
    A 172 Crl 1620 Glioblastoma 1 067 A 204 Htb 82 Rhabdomyosarcoma 1 360 A375 Crl, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    ATCC htb 172 h2170 atcc
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    ATCC nci h209
    Expression of GIRK1 in some SCLC cell lines but not in normal SAE cells, nor in adenocarcinoma Clara cell phenotype cell lines . Top panel: GIRK1 is expressed in WBA, H69, and H146 SCLC cell lines but not in H187, <t>H209</t> and H526 SCLC cell lines. GIRK1 is also not expressed in normal SAE cells, nor is it expressed in H322 or H441 adenocarcinoma Clara cell lines. Bottom panel: Cyclophilin, used as a positive reaction control was seen in all samples. For all gene expression experiments, negative control reactions were performed and found to be negative. The bands on the agarose gels were consistent with the expected sizes: GIRK1-441 bp; cyclophilin-216 bp; M-100 bp.
    Nci H209, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    ATCC nci h209 cell line
    (A) Circos plot representing copy number variations, inter and intra-chromosomal rearrangements of <t>NCI-H209</t> cell line and a xenograft tumour derived from it. Copy number variations (red, gain; green, loss) were calculated based on coverage using the correspondent peripheral blood as control. Inter and intra-chromosomal rearrangements are represented in blue (inter-chromosomal) and dark blue (intra-chromosomal). (B, C) Detailed profile of copy number variations and B-allele frequencies of chromosome 1 from the analysed cell line and xenograft. As described above, the correspondent peripheral blood was used as control for both type of analysis. Copy number profiles are shown in red (gain), green (loss) and grey (no change). LOH are shown light blue.
    Nci H209 Cell Line, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Expression of GIRK1 in some SCLC cell lines but not in normal SAE cells, nor in adenocarcinoma Clara cell phenotype cell lines . Top panel: GIRK1 is expressed in WBA, H69, and H146 SCLC cell lines but not in H187, H209 and H526 SCLC cell lines. GIRK1 is also not expressed in normal SAE cells, nor is it expressed in H322 or H441 adenocarcinoma Clara cell lines. Bottom panel: Cyclophilin, used as a positive reaction control was seen in all samples. For all gene expression experiments, negative control reactions were performed and found to be negative. The bands on the agarose gels were consistent with the expected sizes: GIRK1-441 bp; cyclophilin-216 bp; M-100 bp.

    Journal: BMC Cancer

    Article Title: Expression of G-protein inwardly rectifying potassium channels (GIRKs) in lung cancer cell lines

    doi: 10.1186/1471-2407-5-104

    Figure Lengend Snippet: Expression of GIRK1 in some SCLC cell lines but not in normal SAE cells, nor in adenocarcinoma Clara cell phenotype cell lines . Top panel: GIRK1 is expressed in WBA, H69, and H146 SCLC cell lines but not in H187, H209 and H526 SCLC cell lines. GIRK1 is also not expressed in normal SAE cells, nor is it expressed in H322 or H441 adenocarcinoma Clara cell lines. Bottom panel: Cyclophilin, used as a positive reaction control was seen in all samples. For all gene expression experiments, negative control reactions were performed and found to be negative. The bands on the agarose gels were consistent with the expected sizes: GIRK1-441 bp; cyclophilin-216 bp; M-100 bp.

    Article Snippet: The human SCLC cell lines NCI-H69 (H69), NCI-H146 (H146), NCI-H187 (H187), NCI-H209 (H209), and NCI-H526 (H526), the human adenocarcinoma cell lines NCI-H322 (H322), NCI-H441 (H441), and A549, the carcinoid cell line NCI-H727 (H727), and the squamous cell lines NCI-H226 (H226), NCI-H2170 (H2170), and NCI-H520 (H520) were purchased from the American Type Culture Collection (Manassas, VA).

    Techniques: Expressing, Negative Control

    Expression of GIRK3 in SCLC cell lines . Top panel: GIRK3 expression was seen in all six SCLC cell lines, in WBA, H69, H146, H187, H209, and H526. Bottom panel: Cyclophilin, used as a positive reaction control was seen in all samples. For all gene expression experiments, negative control reactions were performed and found to be negative. The bands on the agarose gels were consistent with the expected sizes: GIRK3-317 bp; cyclophilin-216 bp; M-100 bp.

    Journal: BMC Cancer

    Article Title: Expression of G-protein inwardly rectifying potassium channels (GIRKs) in lung cancer cell lines

    doi: 10.1186/1471-2407-5-104

    Figure Lengend Snippet: Expression of GIRK3 in SCLC cell lines . Top panel: GIRK3 expression was seen in all six SCLC cell lines, in WBA, H69, H146, H187, H209, and H526. Bottom panel: Cyclophilin, used as a positive reaction control was seen in all samples. For all gene expression experiments, negative control reactions were performed and found to be negative. The bands on the agarose gels were consistent with the expected sizes: GIRK3-317 bp; cyclophilin-216 bp; M-100 bp.

    Article Snippet: The human SCLC cell lines NCI-H69 (H69), NCI-H146 (H146), NCI-H187 (H187), NCI-H209 (H209), and NCI-H526 (H526), the human adenocarcinoma cell lines NCI-H322 (H322), NCI-H441 (H441), and A549, the carcinoid cell line NCI-H727 (H727), and the squamous cell lines NCI-H226 (H226), NCI-H2170 (H2170), and NCI-H520 (H520) were purchased from the American Type Culture Collection (Manassas, VA).

    Techniques: Expressing, Negative Control

    Summary of GIRK mRNA expression in lung cells. This is a compilation of data shown in Figures 1–6 & Table 1. Some of the indicated data was not shown.

    Journal: BMC Cancer

    Article Title: Expression of G-protein inwardly rectifying potassium channels (GIRKs) in lung cancer cell lines

    doi: 10.1186/1471-2407-5-104

    Figure Lengend Snippet: Summary of GIRK mRNA expression in lung cells. This is a compilation of data shown in Figures 1–6 & Table 1. Some of the indicated data was not shown.

    Article Snippet: The human SCLC cell lines NCI-H69 (H69), NCI-H146 (H146), NCI-H187 (H187), NCI-H209 (H209), and NCI-H526 (H526), the human adenocarcinoma cell lines NCI-H322 (H322), NCI-H441 (H441), and A549, the carcinoid cell line NCI-H727 (H727), and the squamous cell lines NCI-H226 (H226), NCI-H2170 (H2170), and NCI-H520 (H520) were purchased from the American Type Culture Collection (Manassas, VA).

    Techniques: Expressing

    Expression of GIRK2 or GIRK4 in small cell lung cancer cell lines. Expression of either GIRK2 or GIRK4 was determined by real time PCR. Threshold values (C T ) values are listed below.

    Journal: BMC Cancer

    Article Title: Expression of G-protein inwardly rectifying potassium channels (GIRKs) in lung cancer cell lines

    doi: 10.1186/1471-2407-5-104

    Figure Lengend Snippet: Expression of GIRK2 or GIRK4 in small cell lung cancer cell lines. Expression of either GIRK2 or GIRK4 was determined by real time PCR. Threshold values (C T ) values are listed below.

    Article Snippet: The human SCLC cell lines NCI-H69 (H69), NCI-H146 (H146), NCI-H187 (H187), NCI-H209 (H209), and NCI-H526 (H526), the human adenocarcinoma cell lines NCI-H322 (H322), NCI-H441 (H441), and A549, the carcinoid cell line NCI-H727 (H727), and the squamous cell lines NCI-H226 (H226), NCI-H2170 (H2170), and NCI-H520 (H520) were purchased from the American Type Culture Collection (Manassas, VA).

    Techniques: Expressing, Real-time Polymerase Chain Reaction

    (A) Circos plot representing copy number variations, inter and intra-chromosomal rearrangements of NCI-H209 cell line and a xenograft tumour derived from it. Copy number variations (red, gain; green, loss) were calculated based on coverage using the correspondent peripheral blood as control. Inter and intra-chromosomal rearrangements are represented in blue (inter-chromosomal) and dark blue (intra-chromosomal). (B, C) Detailed profile of copy number variations and B-allele frequencies of chromosome 1 from the analysed cell line and xenograft. As described above, the correspondent peripheral blood was used as control for both type of analysis. Copy number profiles are shown in red (gain), green (loss) and grey (no change). LOH are shown light blue.

    Journal: PLoS ONE

    Article Title: Next-Generation Sequence Analysis of Cancer Xenograft Models

    doi: 10.1371/journal.pone.0074432

    Figure Lengend Snippet: (A) Circos plot representing copy number variations, inter and intra-chromosomal rearrangements of NCI-H209 cell line and a xenograft tumour derived from it. Copy number variations (red, gain; green, loss) were calculated based on coverage using the correspondent peripheral blood as control. Inter and intra-chromosomal rearrangements are represented in blue (inter-chromosomal) and dark blue (intra-chromosomal). (B, C) Detailed profile of copy number variations and B-allele frequencies of chromosome 1 from the analysed cell line and xenograft. As described above, the correspondent peripheral blood was used as control for both type of analysis. Copy number profiles are shown in red (gain), green (loss) and grey (no change). LOH are shown light blue.

    Article Snippet: Authenticated NCI-H209 cell line was purchased from ATCC, re-derived from a single cell clone using the single cell cloning by serial dilution (Corning, Tewksbury, MA, USA) and then cultured in vitro and in vivo as described in Watkins et al. . DNA from samples was extracted using DNAeasy Tissue and Blood Kit (Qiagen, Santa Clara, CA, USA) according to manufacturer’s instructions.

    Techniques: Derivative Assay