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u138  (ATCC)


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    Structured Review

    ATCC u138
    U138, supplied by ATCC, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/u138/product/ATCC
    Average 96 stars, based on 1 article reviews
    u138 - by Bioz Stars, 2025-02
    96/100 stars

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    A Validation of RNAseq data by qRT-PCR. The expression levels of circCLIP2 and its corresponding mRNA indicate the overexpression of circCLIP2 in primary GBM tumors excised from patients (n=12). The expression level of both transcripts is compared to the control of pooled healthy brains. B A positive Pearson’s correlation is calculated for the circCLIP2 and linCLIP2 expression levels in primary GBM tissues. C The genomic loci of the CLIP2 gene and circCLIP2 (hsa_circ_0002755). The red arrow indicates the backsplice site of circCLIP2. D Results from Sanger sequencing of the PCR product of circCLIP2 confirm the presence of backsplice junction sequence. E Visualization of CLIP2 transcripts upon RNase R treatment. F Cytoplasmic localization of circCLIP2 upon subcellular fractionation of U251-MG and <t>U138-MG</t> cells validated by qRT-PCR. *** P ≤ 0.001.
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    A Validation of RNAseq data by qRT-PCR. The expression levels of circCLIP2 and its corresponding mRNA indicate the overexpression of circCLIP2 in primary GBM tumors excised from patients (n=12). The expression level of both transcripts is compared to the control of pooled healthy brains. B A positive Pearson’s correlation is calculated for the circCLIP2 and linCLIP2 expression levels in primary GBM tissues. C The genomic loci of the CLIP2 gene and circCLIP2 (hsa_circ_0002755). The red arrow indicates the backsplice site of circCLIP2. D Results from Sanger sequencing of the PCR product of circCLIP2 confirm the presence of backsplice junction sequence. E Visualization of CLIP2 transcripts upon RNase R treatment. F Cytoplasmic localization of circCLIP2 upon subcellular fractionation of U251-MG and <t>U138-MG</t> cells validated by qRT-PCR. *** P ≤ 0.001.
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    A Validation of RNAseq data by qRT-PCR. The expression levels of circCLIP2 and its corresponding mRNA indicate the overexpression of circCLIP2 in primary GBM tumors excised from patients (n=12). The expression level of both transcripts is compared to the control of pooled healthy brains. B A positive Pearson’s correlation is calculated for the circCLIP2 and linCLIP2 expression levels in primary GBM tissues. C The genomic loci of the CLIP2 gene and circCLIP2 (hsa_circ_0002755). The red arrow indicates the backsplice site of circCLIP2. D Results from Sanger sequencing of the PCR product of circCLIP2 confirm the presence of backsplice junction sequence. E Visualization of CLIP2 transcripts upon RNase R treatment. F Cytoplasmic localization of circCLIP2 upon subcellular fractionation of U251-MG and <t>U138-MG</t> cells validated by qRT-PCR. *** P ≤ 0.001.
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    A Validation of RNAseq data by qRT-PCR. The expression levels of circCLIP2 and its corresponding mRNA indicate the overexpression of circCLIP2 in primary GBM tumors excised from patients (n=12). The expression level of both transcripts is compared to the control of pooled healthy brains. B A positive Pearson’s correlation is calculated for the circCLIP2 and linCLIP2 expression levels in primary GBM tissues. C The genomic loci of the CLIP2 gene and circCLIP2 (hsa_circ_0002755). The red arrow indicates the backsplice site of circCLIP2. D Results from Sanger sequencing of the PCR product of circCLIP2 confirm the presence of backsplice junction sequence. E Visualization of CLIP2 transcripts upon RNase R treatment. F Cytoplasmic localization of circCLIP2 upon subcellular fractionation of U251-MG and U138-MG cells validated by qRT-PCR. *** P ≤ 0.001.

    Journal: bioRxiv

    Article Title: Circular RNA - circCLIP2 is predominantly expressed in GSC niche and enhances glioblastoma aggressiveness via EMT and ECM signaling

    doi: 10.1101/2024.12.01.626287

    Figure Lengend Snippet: A Validation of RNAseq data by qRT-PCR. The expression levels of circCLIP2 and its corresponding mRNA indicate the overexpression of circCLIP2 in primary GBM tumors excised from patients (n=12). The expression level of both transcripts is compared to the control of pooled healthy brains. B A positive Pearson’s correlation is calculated for the circCLIP2 and linCLIP2 expression levels in primary GBM tissues. C The genomic loci of the CLIP2 gene and circCLIP2 (hsa_circ_0002755). The red arrow indicates the backsplice site of circCLIP2. D Results from Sanger sequencing of the PCR product of circCLIP2 confirm the presence of backsplice junction sequence. E Visualization of CLIP2 transcripts upon RNase R treatment. F Cytoplasmic localization of circCLIP2 upon subcellular fractionation of U251-MG and U138-MG cells validated by qRT-PCR. *** P ≤ 0.001.

    Article Snippet: Human glioblastoma cell lines U251-MG and U138-MG were purchased from American Type Culture Collection (ATCC) while U-87 MG from Merck (Sigma).

    Techniques: Quantitative RT-PCR, Expressing, Over Expression, Control, Sequencing, Fractionation

    Upon subcellular fractionation of U251-MG (left panel) and U138-MG (right panel) cell lysates, the levels of GAPDH expression were validated by qRT-PCR. *p ≤ 0.05, **p ≤ 0.01, ****p ≤ 0.0001.

    Journal: bioRxiv

    Article Title: Circular RNA - circCLIP2 is predominantly expressed in GSC niche and enhances glioblastoma aggressiveness via EMT and ECM signaling

    doi: 10.1101/2024.12.01.626287

    Figure Lengend Snippet: Upon subcellular fractionation of U251-MG (left panel) and U138-MG (right panel) cell lysates, the levels of GAPDH expression were validated by qRT-PCR. *p ≤ 0.05, **p ≤ 0.01, ****p ≤ 0.0001.

    Article Snippet: Human glioblastoma cell lines U251-MG and U138-MG were purchased from American Type Culture Collection (ATCC) while U-87 MG from Merck (Sigma).

    Techniques: Fractionation, Expressing, Quantitative RT-PCR

    A Elevated expression levels of circCLIP2 and its corresponding mRNA present in U87-MG and U138-MG cell lines. The expression level of both transcripts is compared to the control of pooled healthy brains. B-F Silencing of linear and circular transcripts of the CLIP2 gene in adherent U87-MG (B) , U138-MG (C) , and U251-MG (E) cell lines as well as derived spheroids from U138-MG (D) and U251-MG (F) cell lines. The specificity of knockdown was established by qRT-PCR analysis and expression levels were normalized to scrambled siRNA (C). *p ≤ 0.05, **p ≤ 0.01, ***p ≤ 0.001, ****p ≤ 0.0001.

    Journal: bioRxiv

    Article Title: Circular RNA - circCLIP2 is predominantly expressed in GSC niche and enhances glioblastoma aggressiveness via EMT and ECM signaling

    doi: 10.1101/2024.12.01.626287

    Figure Lengend Snippet: A Elevated expression levels of circCLIP2 and its corresponding mRNA present in U87-MG and U138-MG cell lines. The expression level of both transcripts is compared to the control of pooled healthy brains. B-F Silencing of linear and circular transcripts of the CLIP2 gene in adherent U87-MG (B) , U138-MG (C) , and U251-MG (E) cell lines as well as derived spheroids from U138-MG (D) and U251-MG (F) cell lines. The specificity of knockdown was established by qRT-PCR analysis and expression levels were normalized to scrambled siRNA (C). *p ≤ 0.05, **p ≤ 0.01, ***p ≤ 0.001, ****p ≤ 0.0001.

    Article Snippet: Human glioblastoma cell lines U251-MG and U138-MG were purchased from American Type Culture Collection (ATCC) while U-87 MG from Merck (Sigma).

    Techniques: Expressing, Control, Derivative Assay, Knockdown, Quantitative RT-PCR

    A and C Proliferation rates were monitored 0-72 h for U251-MG (A) and U138-MG (B) after circCLIP2 or linear CLIP2 knock-downs. B and D Wound healing assay was performed for 0-72h in U251-MG ( B ) and U138-MG ( D ) after circCLIP2 or linear CLIP2 knock-downs. Pictures were captured until the wound was covered by cells transfected with scrambled siRNA (control, C). E-F Expression levels of EMT markers upon silencing of mRNA CLIP2 or circCLIP2 in U251-MG ( E ) and U138-MG ( F ) cell lines established by qRT-PCR. The expression levels were normalized to scrambled siRNA (control, C). G - H Analysis of expression levels of CLIP2 transcripts upon EMT induction in U251-MG adherent cell line ( G ) and U251-MG spheroids ( H ). The results were normalized to untreated adherent U251-MG cells and spheroids (control, C). *p ≤ 0.05, **p ≤ 0.01, ***p ≤ 0.001, ****p ≤ 0.0001.

    Journal: bioRxiv

    Article Title: Circular RNA - circCLIP2 is predominantly expressed in GSC niche and enhances glioblastoma aggressiveness via EMT and ECM signaling

    doi: 10.1101/2024.12.01.626287

    Figure Lengend Snippet: A and C Proliferation rates were monitored 0-72 h for U251-MG (A) and U138-MG (B) after circCLIP2 or linear CLIP2 knock-downs. B and D Wound healing assay was performed for 0-72h in U251-MG ( B ) and U138-MG ( D ) after circCLIP2 or linear CLIP2 knock-downs. Pictures were captured until the wound was covered by cells transfected with scrambled siRNA (control, C). E-F Expression levels of EMT markers upon silencing of mRNA CLIP2 or circCLIP2 in U251-MG ( E ) and U138-MG ( F ) cell lines established by qRT-PCR. The expression levels were normalized to scrambled siRNA (control, C). G - H Analysis of expression levels of CLIP2 transcripts upon EMT induction in U251-MG adherent cell line ( G ) and U251-MG spheroids ( H ). The results were normalized to untreated adherent U251-MG cells and spheroids (control, C). *p ≤ 0.05, **p ≤ 0.01, ***p ≤ 0.001, ****p ≤ 0.0001.

    Article Snippet: Human glioblastoma cell lines U251-MG and U138-MG were purchased from American Type Culture Collection (ATCC) while U-87 MG from Merck (Sigma).

    Techniques: Wound Healing Assay, Transfection, Control, Expressing, Quantitative RT-PCR

    A Expression levels of CLIP2 isoforms in GBM cell lines under hypoxia conditions established by qRT-PCR. The results were normalized to cells grown under normoxia conditions. B Expression levels of CLIP2 in CD133 positive (CD133 + ) and negative (flow-through, FT) fractions isolated from U251-MG spheroids and U138-MG spheroids. C-D Expression level of GSC markers in spheroids derived from U251-MG ( C ) and U138-MG ( D ) upon CLIP2 silencing. E-H. Volume ratio of spheroids derived from U251-MG ( E ) and U138-MG ( F ) grown for 96h upon CLIP2 silencing. Representative microscopy pictures of U251-MG ( G ) and U138-MG ( H ) spheroids taken every 24 h. The results of functional assay were normalized to cells transfected with scrambled siRNA (control, C). *p ≤ 0.05, **p ≤ 0.01, ***p ≤ 0.001, ****p ≤ 0.0001.

    Journal: bioRxiv

    Article Title: Circular RNA - circCLIP2 is predominantly expressed in GSC niche and enhances glioblastoma aggressiveness via EMT and ECM signaling

    doi: 10.1101/2024.12.01.626287

    Figure Lengend Snippet: A Expression levels of CLIP2 isoforms in GBM cell lines under hypoxia conditions established by qRT-PCR. The results were normalized to cells grown under normoxia conditions. B Expression levels of CLIP2 in CD133 positive (CD133 + ) and negative (flow-through, FT) fractions isolated from U251-MG spheroids and U138-MG spheroids. C-D Expression level of GSC markers in spheroids derived from U251-MG ( C ) and U138-MG ( D ) upon CLIP2 silencing. E-H. Volume ratio of spheroids derived from U251-MG ( E ) and U138-MG ( F ) grown for 96h upon CLIP2 silencing. Representative microscopy pictures of U251-MG ( G ) and U138-MG ( H ) spheroids taken every 24 h. The results of functional assay were normalized to cells transfected with scrambled siRNA (control, C). *p ≤ 0.05, **p ≤ 0.01, ***p ≤ 0.001, ****p ≤ 0.0001.

    Article Snippet: Human glioblastoma cell lines U251-MG and U138-MG were purchased from American Type Culture Collection (ATCC) while U-87 MG from Merck (Sigma).

    Techniques: Expressing, Quantitative RT-PCR, Isolation, Derivative Assay, Microscopy, Functional Assay, Transfection, Control

    A The stemness of U251-MG was verified by measuring the protein levels of pluripotency markers in both adherent cells and spheroids using western blotting. B-C Control experiments in which the expression of pluripotency markers was verified in U251-MG- ( B ) and U138-MG- ( C ) derived spheres upon isolating the CD133 + fraction rich in GSCs. The analysis was done by qRT-PCR. D CLIP2 expression shown under normoxia and hypoxia conditions upon CD133 + isolation. Expression evaluation was done by qRT-PCR. *p ≤ 0.05, **p ≤ 0.01.

    Journal: bioRxiv

    Article Title: Circular RNA - circCLIP2 is predominantly expressed in GSC niche and enhances glioblastoma aggressiveness via EMT and ECM signaling

    doi: 10.1101/2024.12.01.626287

    Figure Lengend Snippet: A The stemness of U251-MG was verified by measuring the protein levels of pluripotency markers in both adherent cells and spheroids using western blotting. B-C Control experiments in which the expression of pluripotency markers was verified in U251-MG- ( B ) and U138-MG- ( C ) derived spheres upon isolating the CD133 + fraction rich in GSCs. The analysis was done by qRT-PCR. D CLIP2 expression shown under normoxia and hypoxia conditions upon CD133 + isolation. Expression evaluation was done by qRT-PCR. *p ≤ 0.05, **p ≤ 0.01.

    Article Snippet: Human glioblastoma cell lines U251-MG and U138-MG were purchased from American Type Culture Collection (ATCC) while U-87 MG from Merck (Sigma).

    Techniques: Western Blot, Control, Expressing, Derivative Assay, Quantitative RT-PCR, Isolation