Journal: bioRxiv
Article Title: Circular RNA - circCLIP2 is predominantly expressed in GSC niche and enhances glioblastoma aggressiveness via EMT and ECM signaling
doi: 10.1101/2024.12.01.626287
Figure Lengend Snippet: A Expression levels of CLIP2 isoforms in GBM cell lines under hypoxia conditions established by qRT-PCR. The results were normalized to cells grown under normoxia conditions. B Expression levels of CLIP2 in CD133 positive (CD133 + ) and negative (flow-through, FT) fractions isolated from U251-MG spheroids and U138-MG spheroids. C-D Expression level of GSC markers in spheroids derived from U251-MG ( C ) and U138-MG ( D ) upon CLIP2 silencing. E-H. Volume ratio of spheroids derived from U251-MG ( E ) and U138-MG ( F ) grown for 96h upon CLIP2 silencing. Representative microscopy pictures of U251-MG ( G ) and U138-MG ( H ) spheroids taken every 24 h. The results of functional assay were normalized to cells transfected with scrambled siRNA (control, C). *p ≤ 0.05, **p ≤ 0.01, ***p ≤ 0.001, ****p ≤ 0.0001.
Article Snippet: Human glioblastoma cell lines U251-MG and U138-MG were purchased from American Type Culture Collection (ATCC) while U-87 MG from Merck (Sigma).
Techniques: Expressing, Quantitative RT-PCR, Isolation, Derivative Assay, Microscopy, Functional Assay, Transfection, Control