mda mb 435s  (ATCC)


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    ATCC mda mb 435s
    Characterisation of the investigated breast cancer cell lines
    Mda Mb 435s, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    1) Product Images from "Luminal and basal-like breast cancer cells show increased migration induced by hypoxia, mediated by an autocrine mechanism"

    Article Title: Luminal and basal-like breast cancer cells show increased migration induced by hypoxia, mediated by an autocrine mechanism

    Journal: BMC Cancer

    doi: 10.1186/1471-2407-11-158

    Characterisation of the investigated breast cancer cell lines
    Figure Legend Snippet: Characterisation of the investigated breast cancer cell lines

    Techniques Used: Amplification

    breast mda mb 435 atcc htb 129 cancer mda mb 468 atcc htb 132 bt549 atcc htb 122 hcc1937 atcc crl 2336 hs578t atcc htb 126  (ATCC)


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    ATCC breast mda mb 435 atcc htb 129 cancer mda mb 468 atcc htb 132 bt549 atcc htb 122 hcc1937 atcc crl 2336 hs578t atcc htb 126
    Breast Mda Mb 435 Atcc Htb 129 Cancer Mda Mb 468 Atcc Htb 132 Bt549 Atcc Htb 122 Hcc1937 Atcc Crl 2336 Hs578t Atcc Htb 126, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    mda mb 435s  (ATCC)


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    ATCC mda mb 435s
    Characterisation of the investigated breast cancer cell lines
    Mda Mb 435s, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    1) Product Images from "Luminal and basal-like breast cancer cells show increased migration induced by hypoxia, mediated by an autocrine mechanism"

    Article Title: Luminal and basal-like breast cancer cells show increased migration induced by hypoxia, mediated by an autocrine mechanism

    Journal: BMC Cancer

    doi: 10.1186/1471-2407-11-158

    Characterisation of the investigated breast cancer cell lines
    Figure Legend Snippet: Characterisation of the investigated breast cancer cell lines

    Techniques Used: Amplification

    mda mb 435s  (ATCC)


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    ATCC mda mb 435s
    Agarose gel showing expression of mRNA for GIRK1 in human breast cancer cell lines by RT-PCR . The GIRK1 primers amplified a 441-bp fragment whereas the cyclophylin primers amplified a 216 bp fragment. For each cell line, a negative control reaction without M-MLV reverse transcriptase was performed and found to be negative. Lanes 1 & 7, ZR-75-1; Lanes 2 & 8, MCF-7; Lanes 3 & 9, <t>MDA-MB-361;</t> Lanes 4 & 10, <t>MDA-MB-435S;</t> Lanes 5 & 11, MDA-MB-453; Lanes 6 & 12, MDA-MB-468, Lane M, a 100 bp marker. PCR reactions resolved on this gel were in the plateau phase of PCR, therefore concentrations of PCR amplified cDNA samples cannot be compared.
    Mda Mb 435s, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    1) Product Images from "Expression of inwardly rectifying potassium channels (GIRKs) and beta-adrenergic regulation of breast cancer cell lines"

    Article Title: Expression of inwardly rectifying potassium channels (GIRKs) and beta-adrenergic regulation of breast cancer cell lines

    Journal: BMC Cancer

    doi: 10.1186/1471-2407-4-93

    Agarose gel showing expression of mRNA for GIRK1 in human breast cancer cell lines by RT-PCR . The GIRK1 primers amplified a 441-bp fragment whereas the cyclophylin primers amplified a 216 bp fragment. For each cell line, a negative control reaction without M-MLV reverse transcriptase was performed and found to be negative. Lanes 1 & 7, ZR-75-1; Lanes 2 & 8, MCF-7; Lanes 3 & 9, MDA-MB-361; Lanes 4 & 10, MDA-MB-435S; Lanes 5 & 11, MDA-MB-453; Lanes 6 & 12, MDA-MB-468, Lane M, a 100 bp marker. PCR reactions resolved on this gel were in the plateau phase of PCR, therefore concentrations of PCR amplified cDNA samples cannot be compared.
    Figure Legend Snippet: Agarose gel showing expression of mRNA for GIRK1 in human breast cancer cell lines by RT-PCR . The GIRK1 primers amplified a 441-bp fragment whereas the cyclophylin primers amplified a 216 bp fragment. For each cell line, a negative control reaction without M-MLV reverse transcriptase was performed and found to be negative. Lanes 1 & 7, ZR-75-1; Lanes 2 & 8, MCF-7; Lanes 3 & 9, MDA-MB-361; Lanes 4 & 10, MDA-MB-435S; Lanes 5 & 11, MDA-MB-453; Lanes 6 & 12, MDA-MB-468, Lane M, a 100 bp marker. PCR reactions resolved on this gel were in the plateau phase of PCR, therefore concentrations of PCR amplified cDNA samples cannot be compared.

    Techniques Used: Agarose Gel Electrophoresis, Expressing, Reverse Transcription Polymerase Chain Reaction, Amplification, Negative Control, Marker

    Agarose gel showing expression of mRNA for GIRK2 and GIRK4 in human breast cancer cell lines by RT-PCR . The GIRK2 and 4 primers amplified 438 & 401-bp fragments respectively, whereas the cyclophylin primers amplified a 216 bp fragment. Cyclophylin was used as a positive control for both GIRK2 and 4. For each cell line, a negative control reaction without M-MLV reverse transcriptase was performed and found to be negative. Lanes 1 & 7, ZR-75-1; Lanes 2 & 8, MCF-7; Lanes 3 & 9, MDA-MB-361; Lanes 4 & 10, MDA-MB-435S; Lanes 5 & 11, MDA-MB-453; Lanes 6 & 12, MDA-MB-468, Lane M, a 100 bp marker. PCR reactions resolved on this gel were in the plateau phase of PCR, therefore concentrations of PCR amplified cDNA samples cannot be compared.
    Figure Legend Snippet: Agarose gel showing expression of mRNA for GIRK2 and GIRK4 in human breast cancer cell lines by RT-PCR . The GIRK2 and 4 primers amplified 438 & 401-bp fragments respectively, whereas the cyclophylin primers amplified a 216 bp fragment. Cyclophylin was used as a positive control for both GIRK2 and 4. For each cell line, a negative control reaction without M-MLV reverse transcriptase was performed and found to be negative. Lanes 1 & 7, ZR-75-1; Lanes 2 & 8, MCF-7; Lanes 3 & 9, MDA-MB-361; Lanes 4 & 10, MDA-MB-435S; Lanes 5 & 11, MDA-MB-453; Lanes 6 & 12, MDA-MB-468, Lane M, a 100 bp marker. PCR reactions resolved on this gel were in the plateau phase of PCR, therefore concentrations of PCR amplified cDNA samples cannot be compared.

    Techniques Used: Agarose Gel Electrophoresis, Expressing, Reverse Transcription Polymerase Chain Reaction, Amplification, Positive Control, Negative Control, Marker

    mda mb 435  (ATCC)


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    ATCC mda mb 435
    miR-1193 was significantly downregulated in human breast cancer tissues and cell lines. (A) miR-1193 was downregulated in breast cancer tissues. (B) miR-1193 was decreased in breast cancer cell lines. Breast cancer tissues and paired adjacent noncancerous tissues were sampled from 39 breast cancer patients. The expression levels of miR-1193 were detected in these tissues with real-time qPCR. The expression levels of miR-1193 were detected in human normal breast epithelial cell MCF-10A and human breast cancer cell lines MDA-MB-231, MDA-MB-468, <t>MDA-MB-435,</t> SKBR3, and MCF-7. ** p < 0.01 versus MCF-10A, *** p < 0.001 versus MCF-10A.
    Mda Mb 435, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    1) Product Images from "miR-1193 Suppresses Proliferation and Invasion of Human Breast Cancer Cells Through Directly Targeting IGF2BP2"

    Article Title: miR-1193 Suppresses Proliferation and Invasion of Human Breast Cancer Cells Through Directly Targeting IGF2BP2

    Journal: Oncology Research

    doi: 10.3727/97818823455816X14760504645779

    miR-1193 was significantly downregulated in human breast cancer tissues and cell lines. (A) miR-1193 was downregulated in breast cancer tissues. (B) miR-1193 was decreased in breast cancer cell lines. Breast cancer tissues and paired adjacent noncancerous tissues were sampled from 39 breast cancer patients. The expression levels of miR-1193 were detected in these tissues with real-time qPCR. The expression levels of miR-1193 were detected in human normal breast epithelial cell MCF-10A and human breast cancer cell lines MDA-MB-231, MDA-MB-468, MDA-MB-435, SKBR3, and MCF-7. ** p < 0.01 versus MCF-10A, *** p < 0.001 versus MCF-10A.
    Figure Legend Snippet: miR-1193 was significantly downregulated in human breast cancer tissues and cell lines. (A) miR-1193 was downregulated in breast cancer tissues. (B) miR-1193 was decreased in breast cancer cell lines. Breast cancer tissues and paired adjacent noncancerous tissues were sampled from 39 breast cancer patients. The expression levels of miR-1193 were detected in these tissues with real-time qPCR. The expression levels of miR-1193 were detected in human normal breast epithelial cell MCF-10A and human breast cancer cell lines MDA-MB-231, MDA-MB-468, MDA-MB-435, SKBR3, and MCF-7. ** p < 0.01 versus MCF-10A, *** p < 0.001 versus MCF-10A.

    Techniques Used: Expressing

    mda mb 435s  (ATCC)


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    ATCC mda mb 435s
    Mda Mb 435s, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    ductal carcinoma cell line mda mb 435s  (ATCC)


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    ATCC ductal carcinoma cell line mda mb 435s
    Analysis of EGFR expression levels and binding affinities of sdAbs to human EGFR-presenting cells. Whole-cell lysates of exponentially growing cells (A431, FaDu, <t>MDA-MB</t> <t>435S)</t> were prepared and equal amounts of total cellular proteins were separated by SDS-PAGE on 10% polyacrylamide gels. After Western Blot transfer onto PVDF membranes, EGFR and β-actin proteins were detected by incubation with the respective specific antibodies followed by HRP-coupled antibodies and chemiluminescence detection (A) . In vitro specificity of 99m Tc-7C12 and 99m Tc-EG2 on A431 and FaDu cells was investigated after 1 h incubation on ice (B) . Binding of radiolabeled sdAbs was blocked by 40-fold excess of unlabeled Cetuximab. Binding data is expressed as percent of injected dose per mg protein (% ID/mg protein). NB = non-blocked, B = blocked. For in vitro binding studies, two dimensional cultures of A431, FaDu and MDA-MB 435S cells were incubated with increasing concentrations of 99m Tc-7C12 (C) . Total binding was measured in the absence of and nonspecific binding in the presence of 1 mM unlabeled sdAb. Specific binding was calculated as the difference between total and nonspecific binding. Binding studies were repeated twice and representative saturation curves for the EGFR-positive cell lines A431 and FaDu are shown. For the EGFR-negative cell line MDA-MB 435S, no specific binding was observed.
    Ductal Carcinoma Cell Line Mda Mb 435s, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    1) Product Images from "High-yield production of functional soluble single-domain antibodies in the cytoplasm of Escherichia coli"

    Article Title: High-yield production of functional soluble single-domain antibodies in the cytoplasm of Escherichia coli

    Journal: Microbial Cell Factories

    doi: 10.1186/1475-2859-12-97

    Analysis of EGFR expression levels and binding affinities of sdAbs to human EGFR-presenting cells. Whole-cell lysates of exponentially growing cells (A431, FaDu, MDA-MB 435S) were prepared and equal amounts of total cellular proteins were separated by SDS-PAGE on 10% polyacrylamide gels. After Western Blot transfer onto PVDF membranes, EGFR and β-actin proteins were detected by incubation with the respective specific antibodies followed by HRP-coupled antibodies and chemiluminescence detection (A) . In vitro specificity of 99m Tc-7C12 and 99m Tc-EG2 on A431 and FaDu cells was investigated after 1 h incubation on ice (B) . Binding of radiolabeled sdAbs was blocked by 40-fold excess of unlabeled Cetuximab. Binding data is expressed as percent of injected dose per mg protein (% ID/mg protein). NB = non-blocked, B = blocked. For in vitro binding studies, two dimensional cultures of A431, FaDu and MDA-MB 435S cells were incubated with increasing concentrations of 99m Tc-7C12 (C) . Total binding was measured in the absence of and nonspecific binding in the presence of 1 mM unlabeled sdAb. Specific binding was calculated as the difference between total and nonspecific binding. Binding studies were repeated twice and representative saturation curves for the EGFR-positive cell lines A431 and FaDu are shown. For the EGFR-negative cell line MDA-MB 435S, no specific binding was observed.
    Figure Legend Snippet: Analysis of EGFR expression levels and binding affinities of sdAbs to human EGFR-presenting cells. Whole-cell lysates of exponentially growing cells (A431, FaDu, MDA-MB 435S) were prepared and equal amounts of total cellular proteins were separated by SDS-PAGE on 10% polyacrylamide gels. After Western Blot transfer onto PVDF membranes, EGFR and β-actin proteins were detected by incubation with the respective specific antibodies followed by HRP-coupled antibodies and chemiluminescence detection (A) . In vitro specificity of 99m Tc-7C12 and 99m Tc-EG2 on A431 and FaDu cells was investigated after 1 h incubation on ice (B) . Binding of radiolabeled sdAbs was blocked by 40-fold excess of unlabeled Cetuximab. Binding data is expressed as percent of injected dose per mg protein (% ID/mg protein). NB = non-blocked, B = blocked. For in vitro binding studies, two dimensional cultures of A431, FaDu and MDA-MB 435S cells were incubated with increasing concentrations of 99m Tc-7C12 (C) . Total binding was measured in the absence of and nonspecific binding in the presence of 1 mM unlabeled sdAb. Specific binding was calculated as the difference between total and nonspecific binding. Binding studies were repeated twice and representative saturation curves for the EGFR-positive cell lines A431 and FaDu are shown. For the EGFR-negative cell line MDA-MB 435S, no specific binding was observed.

    Techniques Used: Expressing, Binding Assay, SDS Page, Western Blot, Incubation, In Vitro, Injection

    mda mb 435s cell line  (ATCC)


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    ATCC mda mb 435s cell line
    Identification of a CD44 + /CD24 − subpopulation in breast cancer cell lines MCF-10A, <t>MDA-MB-231,</t> BT-549, and <t>MDA-MB-435S</t> by flow cytometry. Notes: Cells in Q4 correspond to CD44 + /CD24 − cells. MDA-MB-435S shows highest proportion of CD44 + /CD24 − antigen phenotype among other cell lines. Abbreviations: FITC, fluorescein isothiocyanate; PE, phycoerythrin.
    Mda Mb 435s Cell Line, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    1) Product Images from "CD44 + /CD24 − breast cancer cells exhibit phenotypic reversion in three-dimensional self-assembling peptide RADA16 nanofiber scaffold"

    Article Title: CD44 + /CD24 − breast cancer cells exhibit phenotypic reversion in three-dimensional self-assembling peptide RADA16 nanofiber scaffold

    Journal: International Journal of Nanomedicine

    doi: 10.2147/IJN.S66723

    Identification of a CD44 + /CD24 − subpopulation in breast cancer cell lines MCF-10A, MDA-MB-231, BT-549, and MDA-MB-435S by flow cytometry. Notes: Cells in Q4 correspond to CD44 + /CD24 − cells. MDA-MB-435S shows highest proportion of CD44 + /CD24 − antigen phenotype among other cell lines. Abbreviations: FITC, fluorescein isothiocyanate; PE, phycoerythrin.
    Figure Legend Snippet: Identification of a CD44 + /CD24 − subpopulation in breast cancer cell lines MCF-10A, MDA-MB-231, BT-549, and MDA-MB-435S by flow cytometry. Notes: Cells in Q4 correspond to CD44 + /CD24 − cells. MDA-MB-435S shows highest proportion of CD44 + /CD24 − antigen phenotype among other cell lines. Abbreviations: FITC, fluorescein isothiocyanate; PE, phycoerythrin.

    Techniques Used: Flow Cytometry

    The reversion of the malignant phenotype in the morphology of breast cancer cell line MDA-MB-435S in RADA16 compared with Matrigel ® and collagen I scaffolds. Notes: ( A ) AFM images of collagen I, Matrigel, and RADA16 peptide nanofiber scaffolds. Scale bars represent 500 nm. ( B ) Light microscope images (upper) and F-actin and nuclear fluorescence images (lower) of cells encapsulated in collagen I, Matrigel, and RADA16 peptide scaffolds. Three-dimensional cultures were stained for F-actin, and nuclei were counterstained with DAPI. Scale bars represent 100 μm (upper) and 50 μm (lower). ( C ) F-actin and nuclear fluorescence images (upper) and light microscope images (lower) of most common morphology of polarized cell colonies formed in RADA16 scaffold. Scale bars represent 25 μm. ( D ) Encapsulation of cells in different scaffolds using calcein-AM staining for the living cells. Scale bar represents 500 μm. Matrigel ® (BD Biosciences, Two Oak Park, Bedford, MA, USA). Abbreviations: AFM, atomic force microscopy; DAPI, 4′,6-diamidino-2-phenylindole, dihydrochloride; RADA16, COCH 3 -RADARADARADARADA-CONH 2 .
    Figure Legend Snippet: The reversion of the malignant phenotype in the morphology of breast cancer cell line MDA-MB-435S in RADA16 compared with Matrigel ® and collagen I scaffolds. Notes: ( A ) AFM images of collagen I, Matrigel, and RADA16 peptide nanofiber scaffolds. Scale bars represent 500 nm. ( B ) Light microscope images (upper) and F-actin and nuclear fluorescence images (lower) of cells encapsulated in collagen I, Matrigel, and RADA16 peptide scaffolds. Three-dimensional cultures were stained for F-actin, and nuclei were counterstained with DAPI. Scale bars represent 100 μm (upper) and 50 μm (lower). ( C ) F-actin and nuclear fluorescence images (upper) and light microscope images (lower) of most common morphology of polarized cell colonies formed in RADA16 scaffold. Scale bars represent 25 μm. ( D ) Encapsulation of cells in different scaffolds using calcein-AM staining for the living cells. Scale bar represents 500 μm. Matrigel ® (BD Biosciences, Two Oak Park, Bedford, MA, USA). Abbreviations: AFM, atomic force microscopy; DAPI, 4′,6-diamidino-2-phenylindole, dihydrochloride; RADA16, COCH 3 -RADARADARADARADA-CONH 2 .

    Techniques Used: Light Microscopy, Fluorescence, Staining, Microscopy

    The reversion of the malignant phenotype in the proliferation and migration potential of breast cancer cell line MDA-MB-435S in RADA16 compared with Matrigel ® and collagen I scaffolds. Notes: ( A ) Cell density in different scaffolds calculated from DNA measurement after 3-day, 5-day, 7-day, and 9-day culture. ( B ) Percentage of BrdU labeling in cells grown in different scaffolds expressed as the BrdU labeling index from three separate experiments (about 200 cells/experiment) on days 3, 5, 7, and 9. ( C ) Migration potential of MDA-MB-435S cells in Matrigel, collagen I, and RADA16 scaffolds; * P <0.05; ** P <0.001; *** P <0.0001. Matrigel ® (BD Biosciences, Two Oak Park, Bedford, MA, USA). Abbreviations: BrdU, 5-bromo-2′-deoxyuridine; C, collagen; M, Matrigel; R, RADA16; SEM, standard error of the mean; RADA16, COCH 3 -RADARADARADARADA-CONH 2 .
    Figure Legend Snippet: The reversion of the malignant phenotype in the proliferation and migration potential of breast cancer cell line MDA-MB-435S in RADA16 compared with Matrigel ® and collagen I scaffolds. Notes: ( A ) Cell density in different scaffolds calculated from DNA measurement after 3-day, 5-day, 7-day, and 9-day culture. ( B ) Percentage of BrdU labeling in cells grown in different scaffolds expressed as the BrdU labeling index from three separate experiments (about 200 cells/experiment) on days 3, 5, 7, and 9. ( C ) Migration potential of MDA-MB-435S cells in Matrigel, collagen I, and RADA16 scaffolds; * P <0.05; ** P <0.001; *** P <0.0001. Matrigel ® (BD Biosciences, Two Oak Park, Bedford, MA, USA). Abbreviations: BrdU, 5-bromo-2′-deoxyuridine; C, collagen; M, Matrigel; R, RADA16; SEM, standard error of the mean; RADA16, COCH 3 -RADARADARADARADA-CONH 2 .

    Techniques Used: Migration, Labeling

    Cellular characteristics and the localization and expression of signaling proteins in RADA16 cells colonies. Notes: ( A ) Hematoxylin staining of cryostat sections of the Matrigel and RADA16 scaffolds show lumen formation only in RADA16 scaffold. Scale bar represents 25 μm. ( B ) The nuclei of cell colonies stained with DAPI show lumen formation in RADA16 and irregular organization in Matrigel. Scale bar represents 25 μm. ( C ) Immunolocalization of β-catenin (green) with DAPI stained nuclei (blue) in RADA16 and Matrigel scaffolds. The β-catenin is diffusely localized in the cytoplasm, nucleus, and some cell–cell contacts within colonies formed in Matrigel but forms intense localization in the cell–cell junctions in colonies formed in RADA16, indicating similar β-catenin distribution like that of nonmalignant S-1 cells. Scale bar represents 25 μm. ( D ) Western blot analysis of the indicated proteins β-catenin and ICAM-1 of MDA-MB-435S cells in different three-dimensional cultures at 6 days and 9 days. Equal amounts of protein were loaded per lane, and GAPDH was run as a control for equal loading and exposure time. Matrigel ® (BD Biosciences, Two Oak Park, Bedford, MA, USA). Abbreviations: 6 d, 6-day cultures; 9 d, 9-day cultures; C, collagen; DAPI, 4′,6-diamidino-2-phenylindole, dihydrochloride; GAPDH, glyceraldehyde-3-phosphate dehydrogenase; ICAM-1, intercellular surface adhesion molecule-1; M, Matrigel; R, RADA16; RADA16, COCH 3 -RADARADARADARADA-CONH 2 .
    Figure Legend Snippet: Cellular characteristics and the localization and expression of signaling proteins in RADA16 cells colonies. Notes: ( A ) Hematoxylin staining of cryostat sections of the Matrigel and RADA16 scaffolds show lumen formation only in RADA16 scaffold. Scale bar represents 25 μm. ( B ) The nuclei of cell colonies stained with DAPI show lumen formation in RADA16 and irregular organization in Matrigel. Scale bar represents 25 μm. ( C ) Immunolocalization of β-catenin (green) with DAPI stained nuclei (blue) in RADA16 and Matrigel scaffolds. The β-catenin is diffusely localized in the cytoplasm, nucleus, and some cell–cell contacts within colonies formed in Matrigel but forms intense localization in the cell–cell junctions in colonies formed in RADA16, indicating similar β-catenin distribution like that of nonmalignant S-1 cells. Scale bar represents 25 μm. ( D ) Western blot analysis of the indicated proteins β-catenin and ICAM-1 of MDA-MB-435S cells in different three-dimensional cultures at 6 days and 9 days. Equal amounts of protein were loaded per lane, and GAPDH was run as a control for equal loading and exposure time. Matrigel ® (BD Biosciences, Two Oak Park, Bedford, MA, USA). Abbreviations: 6 d, 6-day cultures; 9 d, 9-day cultures; C, collagen; DAPI, 4′,6-diamidino-2-phenylindole, dihydrochloride; GAPDH, glyceraldehyde-3-phosphate dehydrogenase; ICAM-1, intercellular surface adhesion molecule-1; M, Matrigel; R, RADA16; RADA16, COCH 3 -RADARADARADARADA-CONH 2 .

    Techniques Used: Expressing, Staining, Western Blot

    The reversion phenotype of MDA-MB-435S cells was inhibited by blocking NF-κB signaling by PDTC. Notes: ( A ) Western blot analysis of ICAM-1 of MDA-MB-435S cells in different culture groups. Equal amounts of protein were loaded per lane, and GAPDH was run as a control for equal loading and exposure time. The expression of ICAM-1 of cells in RADA16 was significantly downregulated by PDTC (* P <0.05). ( B ) Light microscope images and F-actin and nuclear fluorescence images of cells in PDTC-treated RADA16 group. Three-dimensional cultures were stained for F-actin, and nuclei were counterstained with DAPI. Scale bars represent 100 μm (left) and 50 μm (right). Abbreviations: 2D, two dimensional group; DAPI, 4′,6-diamidino-2-phenylindole, dihydrochloride; GAPDH, glyceraldehyde-3-phosphate dehydrogenase; ICAM-1, intercellular surface adhesion molecule-1; PDTC, pyrrolidine dithiocarbamate; R, RADA16 group; Rp, RADA16 + PDTC group; RADA16, COCH 3 -RADARADAR-ADARADA-CONH 2 .
    Figure Legend Snippet: The reversion phenotype of MDA-MB-435S cells was inhibited by blocking NF-κB signaling by PDTC. Notes: ( A ) Western blot analysis of ICAM-1 of MDA-MB-435S cells in different culture groups. Equal amounts of protein were loaded per lane, and GAPDH was run as a control for equal loading and exposure time. The expression of ICAM-1 of cells in RADA16 was significantly downregulated by PDTC (* P <0.05). ( B ) Light microscope images and F-actin and nuclear fluorescence images of cells in PDTC-treated RADA16 group. Three-dimensional cultures were stained for F-actin, and nuclei were counterstained with DAPI. Scale bars represent 100 μm (left) and 50 μm (right). Abbreviations: 2D, two dimensional group; DAPI, 4′,6-diamidino-2-phenylindole, dihydrochloride; GAPDH, glyceraldehyde-3-phosphate dehydrogenase; ICAM-1, intercellular surface adhesion molecule-1; PDTC, pyrrolidine dithiocarbamate; R, RADA16 group; Rp, RADA16 + PDTC group; RADA16, COCH 3 -RADARADAR-ADARADA-CONH 2 .

    Techniques Used: Blocking Assay, Western Blot, Expressing, Light Microscopy, Fluorescence, Staining

    mda mb 435s  (ATCC)


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    ATCC mda mb 435s
    PHGDH and PSPH levels were increased in the TNBC cell lines and the GLDC levels was increased in <t>MDA-MB-453</t> of the HER-2 subtype and <t>MDA-MB-435S</t> of the TNBC subtype. SHMT1 was highly expressed in the HER-2 subtype and MDA-MB-435S cells.
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    1) Product Images from "Differential Expression of Enzymes Associated with Serine/Glycine Metabolism in Different Breast Cancer Subtypes"

    Article Title: Differential Expression of Enzymes Associated with Serine/Glycine Metabolism in Different Breast Cancer Subtypes

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0101004

    PHGDH and PSPH levels were increased in the TNBC cell lines and the GLDC levels was increased in MDA-MB-453 of the HER-2 subtype and MDA-MB-435S of the TNBC subtype. SHMT1 was highly expressed in the HER-2 subtype and MDA-MB-435S cells.
    Figure Legend Snippet: PHGDH and PSPH levels were increased in the TNBC cell lines and the GLDC levels was increased in MDA-MB-453 of the HER-2 subtype and MDA-MB-435S of the TNBC subtype. SHMT1 was highly expressed in the HER-2 subtype and MDA-MB-435S cells.

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    mda mb 435s  (ATCC)


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    ATCC mda mb 435s
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    breast cancer cell line mda mb  (ATCC)


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    ATCC breast cancer cell line mda mb
    Breast Cancer Cell Line Mda Mb, supplied by ATCC, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    ATCC mda mb 435s
    Characterisation of the investigated breast cancer cell lines
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    ATCC breast mda mb 435 atcc htb 129 cancer mda mb 468 atcc htb 132 bt549 atcc htb 122 hcc1937 atcc crl 2336 hs578t atcc htb 126
    Characterisation of the investigated breast cancer cell lines
    Breast Mda Mb 435 Atcc Htb 129 Cancer Mda Mb 468 Atcc Htb 132 Bt549 Atcc Htb 122 Hcc1937 Atcc Crl 2336 Hs578t Atcc Htb 126, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    ATCC mda mb 435
    miR-1193 was significantly downregulated in human breast cancer tissues and cell lines. (A) miR-1193 was downregulated in breast cancer tissues. (B) miR-1193 was decreased in breast cancer cell lines. Breast cancer tissues and paired adjacent noncancerous tissues were sampled from 39 breast cancer patients. The expression levels of miR-1193 were detected in these tissues with real-time qPCR. The expression levels of miR-1193 were detected in human normal breast epithelial cell MCF-10A and human breast cancer cell lines MDA-MB-231, MDA-MB-468, <t>MDA-MB-435,</t> SKBR3, and MCF-7. ** p < 0.01 versus MCF-10A, *** p < 0.001 versus MCF-10A.
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    ATCC ductal carcinoma cell line mda mb 435s
    Analysis of EGFR expression levels and binding affinities of sdAbs to human EGFR-presenting cells. Whole-cell lysates of exponentially growing cells (A431, FaDu, <t>MDA-MB</t> <t>435S)</t> were prepared and equal amounts of total cellular proteins were separated by SDS-PAGE on 10% polyacrylamide gels. After Western Blot transfer onto PVDF membranes, EGFR and β-actin proteins were detected by incubation with the respective specific antibodies followed by HRP-coupled antibodies and chemiluminescence detection (A) . In vitro specificity of 99m Tc-7C12 and 99m Tc-EG2 on A431 and FaDu cells was investigated after 1 h incubation on ice (B) . Binding of radiolabeled sdAbs was blocked by 40-fold excess of unlabeled Cetuximab. Binding data is expressed as percent of injected dose per mg protein (% ID/mg protein). NB = non-blocked, B = blocked. For in vitro binding studies, two dimensional cultures of A431, FaDu and MDA-MB 435S cells were incubated with increasing concentrations of 99m Tc-7C12 (C) . Total binding was measured in the absence of and nonspecific binding in the presence of 1 mM unlabeled sdAb. Specific binding was calculated as the difference between total and nonspecific binding. Binding studies were repeated twice and representative saturation curves for the EGFR-positive cell lines A431 and FaDu are shown. For the EGFR-negative cell line MDA-MB 435S, no specific binding was observed.
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    ATCC mda mb 435s cell line
    Identification of a CD44 + /CD24 − subpopulation in breast cancer cell lines MCF-10A, <t>MDA-MB-231,</t> BT-549, and <t>MDA-MB-435S</t> by flow cytometry. Notes: Cells in Q4 correspond to CD44 + /CD24 − cells. MDA-MB-435S shows highest proportion of CD44 + /CD24 − antigen phenotype among other cell lines. Abbreviations: FITC, fluorescein isothiocyanate; PE, phycoerythrin.
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    ATCC breast cancer cell line mda mb
    Identification of a CD44 + /CD24 − subpopulation in breast cancer cell lines MCF-10A, <t>MDA-MB-231,</t> BT-549, and <t>MDA-MB-435S</t> by flow cytometry. Notes: Cells in Q4 correspond to CD44 + /CD24 − cells. MDA-MB-435S shows highest proportion of CD44 + /CD24 − antigen phenotype among other cell lines. Abbreviations: FITC, fluorescein isothiocyanate; PE, phycoerythrin.
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    Characterisation of the investigated breast cancer cell lines

    Journal: BMC Cancer

    Article Title: Luminal and basal-like breast cancer cells show increased migration induced by hypoxia, mediated by an autocrine mechanism

    doi: 10.1186/1471-2407-11-158

    Figure Lengend Snippet: Characterisation of the investigated breast cancer cell lines

    Article Snippet: The cell line MCF-7 was derived from the German Collection of Microorganisms and Cell Cultures (DSMZ, Braunschweig, Germany), the cell lines MDA-MB-231, MDA-MB-435S and MDA-MB-468 were derived from the American Type Culture Collection (ATCC, Manassas, VA).

    Techniques: Amplification

    miR-1193 was significantly downregulated in human breast cancer tissues and cell lines. (A) miR-1193 was downregulated in breast cancer tissues. (B) miR-1193 was decreased in breast cancer cell lines. Breast cancer tissues and paired adjacent noncancerous tissues were sampled from 39 breast cancer patients. The expression levels of miR-1193 were detected in these tissues with real-time qPCR. The expression levels of miR-1193 were detected in human normal breast epithelial cell MCF-10A and human breast cancer cell lines MDA-MB-231, MDA-MB-468, MDA-MB-435, SKBR3, and MCF-7. ** p < 0.01 versus MCF-10A, *** p < 0.001 versus MCF-10A.

    Journal: Oncology Research

    Article Title: miR-1193 Suppresses Proliferation and Invasion of Human Breast Cancer Cells Through Directly Targeting IGF2BP2

    doi: 10.3727/97818823455816X14760504645779

    Figure Lengend Snippet: miR-1193 was significantly downregulated in human breast cancer tissues and cell lines. (A) miR-1193 was downregulated in breast cancer tissues. (B) miR-1193 was decreased in breast cancer cell lines. Breast cancer tissues and paired adjacent noncancerous tissues were sampled from 39 breast cancer patients. The expression levels of miR-1193 were detected in these tissues with real-time qPCR. The expression levels of miR-1193 were detected in human normal breast epithelial cell MCF-10A and human breast cancer cell lines MDA-MB-231, MDA-MB-468, MDA-MB-435, SKBR3, and MCF-7. ** p < 0.01 versus MCF-10A, *** p < 0.001 versus MCF-10A.

    Article Snippet: Human normal breast epithelial cell MCF-10A; human breast cancer cell lines, including MDA-MB-231, MDA-MB-468, MDA-MB-435, SKBR3, and MCF-7; and HEK293 cells were purchased from the American Type Culture Collection Company (ATCC; Manassas, VA, USA).

    Techniques: Expressing

    Analysis of EGFR expression levels and binding affinities of sdAbs to human EGFR-presenting cells. Whole-cell lysates of exponentially growing cells (A431, FaDu, MDA-MB 435S) were prepared and equal amounts of total cellular proteins were separated by SDS-PAGE on 10% polyacrylamide gels. After Western Blot transfer onto PVDF membranes, EGFR and β-actin proteins were detected by incubation with the respective specific antibodies followed by HRP-coupled antibodies and chemiluminescence detection (A) . In vitro specificity of 99m Tc-7C12 and 99m Tc-EG2 on A431 and FaDu cells was investigated after 1 h incubation on ice (B) . Binding of radiolabeled sdAbs was blocked by 40-fold excess of unlabeled Cetuximab. Binding data is expressed as percent of injected dose per mg protein (% ID/mg protein). NB = non-blocked, B = blocked. For in vitro binding studies, two dimensional cultures of A431, FaDu and MDA-MB 435S cells were incubated with increasing concentrations of 99m Tc-7C12 (C) . Total binding was measured in the absence of and nonspecific binding in the presence of 1 mM unlabeled sdAb. Specific binding was calculated as the difference between total and nonspecific binding. Binding studies were repeated twice and representative saturation curves for the EGFR-positive cell lines A431 and FaDu are shown. For the EGFR-negative cell line MDA-MB 435S, no specific binding was observed.

    Journal: Microbial Cell Factories

    Article Title: High-yield production of functional soluble single-domain antibodies in the cytoplasm of Escherichia coli

    doi: 10.1186/1475-2859-12-97

    Figure Lengend Snippet: Analysis of EGFR expression levels and binding affinities of sdAbs to human EGFR-presenting cells. Whole-cell lysates of exponentially growing cells (A431, FaDu, MDA-MB 435S) were prepared and equal amounts of total cellular proteins were separated by SDS-PAGE on 10% polyacrylamide gels. After Western Blot transfer onto PVDF membranes, EGFR and β-actin proteins were detected by incubation with the respective specific antibodies followed by HRP-coupled antibodies and chemiluminescence detection (A) . In vitro specificity of 99m Tc-7C12 and 99m Tc-EG2 on A431 and FaDu cells was investigated after 1 h incubation on ice (B) . Binding of radiolabeled sdAbs was blocked by 40-fold excess of unlabeled Cetuximab. Binding data is expressed as percent of injected dose per mg protein (% ID/mg protein). NB = non-blocked, B = blocked. For in vitro binding studies, two dimensional cultures of A431, FaDu and MDA-MB 435S cells were incubated with increasing concentrations of 99m Tc-7C12 (C) . Total binding was measured in the absence of and nonspecific binding in the presence of 1 mM unlabeled sdAb. Specific binding was calculated as the difference between total and nonspecific binding. Binding studies were repeated twice and representative saturation curves for the EGFR-positive cell lines A431 and FaDu are shown. For the EGFR-negative cell line MDA-MB 435S, no specific binding was observed.

    Article Snippet: For binding and uptake studies, three different adherent human tumor cell lines were used: the epidermoid carcinoma cell line A431 (ATCC® Number: CRL-1555), the squamous cell carcinoma cell line FaDu (ATCC® Number: HTB-43), the ductal carcinoma cell line MDA-MB 435S (ATCC® Number: HTB-129).

    Techniques: Expressing, Binding Assay, SDS Page, Western Blot, Incubation, In Vitro, Injection

    Identification of a CD44 + /CD24 − subpopulation in breast cancer cell lines MCF-10A, MDA-MB-231, BT-549, and MDA-MB-435S by flow cytometry. Notes: Cells in Q4 correspond to CD44 + /CD24 − cells. MDA-MB-435S shows highest proportion of CD44 + /CD24 − antigen phenotype among other cell lines. Abbreviations: FITC, fluorescein isothiocyanate; PE, phycoerythrin.

    Journal: International Journal of Nanomedicine

    Article Title: CD44 + /CD24 − breast cancer cells exhibit phenotypic reversion in three-dimensional self-assembling peptide RADA16 nanofiber scaffold

    doi: 10.2147/IJN.S66723

    Figure Lengend Snippet: Identification of a CD44 + /CD24 − subpopulation in breast cancer cell lines MCF-10A, MDA-MB-231, BT-549, and MDA-MB-435S by flow cytometry. Notes: Cells in Q4 correspond to CD44 + /CD24 − cells. MDA-MB-435S shows highest proportion of CD44 + /CD24 − antigen phenotype among other cell lines. Abbreviations: FITC, fluorescein isothiocyanate; PE, phycoerythrin.

    Article Snippet: The MDA-MB-435S cell line was obtained from the American Type Culture Collection (Manassas, VA, USA).

    Techniques: Flow Cytometry

    The reversion of the malignant phenotype in the morphology of breast cancer cell line MDA-MB-435S in RADA16 compared with Matrigel ® and collagen I scaffolds. Notes: ( A ) AFM images of collagen I, Matrigel, and RADA16 peptide nanofiber scaffolds. Scale bars represent 500 nm. ( B ) Light microscope images (upper) and F-actin and nuclear fluorescence images (lower) of cells encapsulated in collagen I, Matrigel, and RADA16 peptide scaffolds. Three-dimensional cultures were stained for F-actin, and nuclei were counterstained with DAPI. Scale bars represent 100 μm (upper) and 50 μm (lower). ( C ) F-actin and nuclear fluorescence images (upper) and light microscope images (lower) of most common morphology of polarized cell colonies formed in RADA16 scaffold. Scale bars represent 25 μm. ( D ) Encapsulation of cells in different scaffolds using calcein-AM staining for the living cells. Scale bar represents 500 μm. Matrigel ® (BD Biosciences, Two Oak Park, Bedford, MA, USA). Abbreviations: AFM, atomic force microscopy; DAPI, 4′,6-diamidino-2-phenylindole, dihydrochloride; RADA16, COCH 3 -RADARADARADARADA-CONH 2 .

    Journal: International Journal of Nanomedicine

    Article Title: CD44 + /CD24 − breast cancer cells exhibit phenotypic reversion in three-dimensional self-assembling peptide RADA16 nanofiber scaffold

    doi: 10.2147/IJN.S66723

    Figure Lengend Snippet: The reversion of the malignant phenotype in the morphology of breast cancer cell line MDA-MB-435S in RADA16 compared with Matrigel ® and collagen I scaffolds. Notes: ( A ) AFM images of collagen I, Matrigel, and RADA16 peptide nanofiber scaffolds. Scale bars represent 500 nm. ( B ) Light microscope images (upper) and F-actin and nuclear fluorescence images (lower) of cells encapsulated in collagen I, Matrigel, and RADA16 peptide scaffolds. Three-dimensional cultures were stained for F-actin, and nuclei were counterstained with DAPI. Scale bars represent 100 μm (upper) and 50 μm (lower). ( C ) F-actin and nuclear fluorescence images (upper) and light microscope images (lower) of most common morphology of polarized cell colonies formed in RADA16 scaffold. Scale bars represent 25 μm. ( D ) Encapsulation of cells in different scaffolds using calcein-AM staining for the living cells. Scale bar represents 500 μm. Matrigel ® (BD Biosciences, Two Oak Park, Bedford, MA, USA). Abbreviations: AFM, atomic force microscopy; DAPI, 4′,6-diamidino-2-phenylindole, dihydrochloride; RADA16, COCH 3 -RADARADARADARADA-CONH 2 .

    Article Snippet: The MDA-MB-435S cell line was obtained from the American Type Culture Collection (Manassas, VA, USA).

    Techniques: Light Microscopy, Fluorescence, Staining, Microscopy

    The reversion of the malignant phenotype in the proliferation and migration potential of breast cancer cell line MDA-MB-435S in RADA16 compared with Matrigel ® and collagen I scaffolds. Notes: ( A ) Cell density in different scaffolds calculated from DNA measurement after 3-day, 5-day, 7-day, and 9-day culture. ( B ) Percentage of BrdU labeling in cells grown in different scaffolds expressed as the BrdU labeling index from three separate experiments (about 200 cells/experiment) on days 3, 5, 7, and 9. ( C ) Migration potential of MDA-MB-435S cells in Matrigel, collagen I, and RADA16 scaffolds; * P <0.05; ** P <0.001; *** P <0.0001. Matrigel ® (BD Biosciences, Two Oak Park, Bedford, MA, USA). Abbreviations: BrdU, 5-bromo-2′-deoxyuridine; C, collagen; M, Matrigel; R, RADA16; SEM, standard error of the mean; RADA16, COCH 3 -RADARADARADARADA-CONH 2 .

    Journal: International Journal of Nanomedicine

    Article Title: CD44 + /CD24 − breast cancer cells exhibit phenotypic reversion in three-dimensional self-assembling peptide RADA16 nanofiber scaffold

    doi: 10.2147/IJN.S66723

    Figure Lengend Snippet: The reversion of the malignant phenotype in the proliferation and migration potential of breast cancer cell line MDA-MB-435S in RADA16 compared with Matrigel ® and collagen I scaffolds. Notes: ( A ) Cell density in different scaffolds calculated from DNA measurement after 3-day, 5-day, 7-day, and 9-day culture. ( B ) Percentage of BrdU labeling in cells grown in different scaffolds expressed as the BrdU labeling index from three separate experiments (about 200 cells/experiment) on days 3, 5, 7, and 9. ( C ) Migration potential of MDA-MB-435S cells in Matrigel, collagen I, and RADA16 scaffolds; * P <0.05; ** P <0.001; *** P <0.0001. Matrigel ® (BD Biosciences, Two Oak Park, Bedford, MA, USA). Abbreviations: BrdU, 5-bromo-2′-deoxyuridine; C, collagen; M, Matrigel; R, RADA16; SEM, standard error of the mean; RADA16, COCH 3 -RADARADARADARADA-CONH 2 .

    Article Snippet: The MDA-MB-435S cell line was obtained from the American Type Culture Collection (Manassas, VA, USA).

    Techniques: Migration, Labeling

    Cellular characteristics and the localization and expression of signaling proteins in RADA16 cells colonies. Notes: ( A ) Hematoxylin staining of cryostat sections of the Matrigel and RADA16 scaffolds show lumen formation only in RADA16 scaffold. Scale bar represents 25 μm. ( B ) The nuclei of cell colonies stained with DAPI show lumen formation in RADA16 and irregular organization in Matrigel. Scale bar represents 25 μm. ( C ) Immunolocalization of β-catenin (green) with DAPI stained nuclei (blue) in RADA16 and Matrigel scaffolds. The β-catenin is diffusely localized in the cytoplasm, nucleus, and some cell–cell contacts within colonies formed in Matrigel but forms intense localization in the cell–cell junctions in colonies formed in RADA16, indicating similar β-catenin distribution like that of nonmalignant S-1 cells. Scale bar represents 25 μm. ( D ) Western blot analysis of the indicated proteins β-catenin and ICAM-1 of MDA-MB-435S cells in different three-dimensional cultures at 6 days and 9 days. Equal amounts of protein were loaded per lane, and GAPDH was run as a control for equal loading and exposure time. Matrigel ® (BD Biosciences, Two Oak Park, Bedford, MA, USA). Abbreviations: 6 d, 6-day cultures; 9 d, 9-day cultures; C, collagen; DAPI, 4′,6-diamidino-2-phenylindole, dihydrochloride; GAPDH, glyceraldehyde-3-phosphate dehydrogenase; ICAM-1, intercellular surface adhesion molecule-1; M, Matrigel; R, RADA16; RADA16, COCH 3 -RADARADARADARADA-CONH 2 .

    Journal: International Journal of Nanomedicine

    Article Title: CD44 + /CD24 − breast cancer cells exhibit phenotypic reversion in three-dimensional self-assembling peptide RADA16 nanofiber scaffold

    doi: 10.2147/IJN.S66723

    Figure Lengend Snippet: Cellular characteristics and the localization and expression of signaling proteins in RADA16 cells colonies. Notes: ( A ) Hematoxylin staining of cryostat sections of the Matrigel and RADA16 scaffolds show lumen formation only in RADA16 scaffold. Scale bar represents 25 μm. ( B ) The nuclei of cell colonies stained with DAPI show lumen formation in RADA16 and irregular organization in Matrigel. Scale bar represents 25 μm. ( C ) Immunolocalization of β-catenin (green) with DAPI stained nuclei (blue) in RADA16 and Matrigel scaffolds. The β-catenin is diffusely localized in the cytoplasm, nucleus, and some cell–cell contacts within colonies formed in Matrigel but forms intense localization in the cell–cell junctions in colonies formed in RADA16, indicating similar β-catenin distribution like that of nonmalignant S-1 cells. Scale bar represents 25 μm. ( D ) Western blot analysis of the indicated proteins β-catenin and ICAM-1 of MDA-MB-435S cells in different three-dimensional cultures at 6 days and 9 days. Equal amounts of protein were loaded per lane, and GAPDH was run as a control for equal loading and exposure time. Matrigel ® (BD Biosciences, Two Oak Park, Bedford, MA, USA). Abbreviations: 6 d, 6-day cultures; 9 d, 9-day cultures; C, collagen; DAPI, 4′,6-diamidino-2-phenylindole, dihydrochloride; GAPDH, glyceraldehyde-3-phosphate dehydrogenase; ICAM-1, intercellular surface adhesion molecule-1; M, Matrigel; R, RADA16; RADA16, COCH 3 -RADARADARADARADA-CONH 2 .

    Article Snippet: The MDA-MB-435S cell line was obtained from the American Type Culture Collection (Manassas, VA, USA).

    Techniques: Expressing, Staining, Western Blot

    The reversion phenotype of MDA-MB-435S cells was inhibited by blocking NF-κB signaling by PDTC. Notes: ( A ) Western blot analysis of ICAM-1 of MDA-MB-435S cells in different culture groups. Equal amounts of protein were loaded per lane, and GAPDH was run as a control for equal loading and exposure time. The expression of ICAM-1 of cells in RADA16 was significantly downregulated by PDTC (* P <0.05). ( B ) Light microscope images and F-actin and nuclear fluorescence images of cells in PDTC-treated RADA16 group. Three-dimensional cultures were stained for F-actin, and nuclei were counterstained with DAPI. Scale bars represent 100 μm (left) and 50 μm (right). Abbreviations: 2D, two dimensional group; DAPI, 4′,6-diamidino-2-phenylindole, dihydrochloride; GAPDH, glyceraldehyde-3-phosphate dehydrogenase; ICAM-1, intercellular surface adhesion molecule-1; PDTC, pyrrolidine dithiocarbamate; R, RADA16 group; Rp, RADA16 + PDTC group; RADA16, COCH 3 -RADARADAR-ADARADA-CONH 2 .

    Journal: International Journal of Nanomedicine

    Article Title: CD44 + /CD24 − breast cancer cells exhibit phenotypic reversion in three-dimensional self-assembling peptide RADA16 nanofiber scaffold

    doi: 10.2147/IJN.S66723

    Figure Lengend Snippet: The reversion phenotype of MDA-MB-435S cells was inhibited by blocking NF-κB signaling by PDTC. Notes: ( A ) Western blot analysis of ICAM-1 of MDA-MB-435S cells in different culture groups. Equal amounts of protein were loaded per lane, and GAPDH was run as a control for equal loading and exposure time. The expression of ICAM-1 of cells in RADA16 was significantly downregulated by PDTC (* P <0.05). ( B ) Light microscope images and F-actin and nuclear fluorescence images of cells in PDTC-treated RADA16 group. Three-dimensional cultures were stained for F-actin, and nuclei were counterstained with DAPI. Scale bars represent 100 μm (left) and 50 μm (right). Abbreviations: 2D, two dimensional group; DAPI, 4′,6-diamidino-2-phenylindole, dihydrochloride; GAPDH, glyceraldehyde-3-phosphate dehydrogenase; ICAM-1, intercellular surface adhesion molecule-1; PDTC, pyrrolidine dithiocarbamate; R, RADA16 group; Rp, RADA16 + PDTC group; RADA16, COCH 3 -RADARADAR-ADARADA-CONH 2 .

    Article Snippet: The MDA-MB-435S cell line was obtained from the American Type Culture Collection (Manassas, VA, USA).

    Techniques: Blocking Assay, Western Blot, Expressing, Light Microscopy, Fluorescence, Staining