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ATCC hec 1 a
Hec 1 A, supplied by ATCC, used in various techniques. Bioz Stars score: 96/100, based on 823 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/hec 1 a/product/ATCC
Average 96 stars, based on 823 article reviews

hec 1 a - by Bioz Stars, 2026-05

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(A, B) Kaplan-Meier plots showing that LARP1 overexpression is associated with shorter overall survival (A) and progression-free interval (B) in endometrial cancer cohort (n=454). (C) Immunoblot analysis illustrating the protein expression of LARP1 after transfecting ISHI cell line with control or LARP1 siRNA at 25, 50, 75, and 100 nM. β-actin was used as a loading control. (D) Images derived from immunofluorescence analysis showing the protein expression of LARP1 after transfecting HEC-1A cells with control or LARP1 siRNA at 75 µM. Scale bar = 50 µM.

Journal: bioRxiv

Article Title: “Targeting LARP1 Enhances Carboplatin Sensitivity and Suppresses Tumor Growth in Endometrial Cancer”

doi: 10.64898/2026.03.22.713473

Figure Lengend Snippet: (A, B) Kaplan-Meier plots showing that LARP1 overexpression is associated with shorter overall survival (A) and progression-free interval (B) in endometrial cancer cohort (n=454). (C) Immunoblot analysis illustrating the protein expression of LARP1 after transfecting ISHI cell line with control or LARP1 siRNA at 25, 50, 75, and 100 nM. β-actin was used as a loading control. (D) Images derived from immunofluorescence analysis showing the protein expression of LARP1 after transfecting HEC-1A cells with control or LARP1 siRNA at 75 µM. Scale bar = 50 µM.

Article Snippet: Human endometrial cancer cell lines HEC-1A and Ishikawa (ISHI) were obtained from ATCC (American Type Culture Collection, Manassas, VA, USA).

Techniques: Over Expression, Western Blot, Expressing, Control, Derivative Assay, Immunofluorescence

(A – D) Cell viability assay showing the effect of two different LARP1 siRNA (1 and 2) on the viability of ISHI (A, B) and HEC-1A (C, D) cell lines. One-way analysis of variance (ANOVA) was used to assess statistical significance among groups, followed by Tukey’s post hoc test for multiple pairwise comparisons. Data are presented as mean ± SD, with individual data points shown as dots. (E) Immunoblot analysis showing the protein expression of cleaved PARP1 and cleaved caspase 3 after transfection of ISHI and HEC-1A cells with control or LARP1 siRNAs. β-actin was used as a loading control.

Journal: bioRxiv

Article Title: “Targeting LARP1 Enhances Carboplatin Sensitivity and Suppresses Tumor Growth in Endometrial Cancer”

doi: 10.64898/2026.03.22.713473

Figure Lengend Snippet: (A – D) Cell viability assay showing the effect of two different LARP1 siRNA (1 and 2) on the viability of ISHI (A, B) and HEC-1A (C, D) cell lines. One-way analysis of variance (ANOVA) was used to assess statistical significance among groups, followed by Tukey’s post hoc test for multiple pairwise comparisons. Data are presented as mean ± SD, with individual data points shown as dots. (E) Immunoblot analysis showing the protein expression of cleaved PARP1 and cleaved caspase 3 after transfection of ISHI and HEC-1A cells with control or LARP1 siRNAs. β-actin was used as a loading control.

Article Snippet: Human endometrial cancer cell lines HEC-1A and Ishikawa (ISHI) were obtained from ATCC (American Type Culture Collection, Manassas, VA, USA).

Techniques: Viability Assay, Western Blot, Expressing, Transfection, Control

(A, B) Immunoblot analysis showing the protein expression of LARP1 and E2F1 after transfecting ISHI (A) and HEC-1A (B) cells with control or LARP1 siRNA. β-actin was used as a loading control. (C) Images representing immunofluorescence staining of LARP1 (green) and E2F1 (red) in HEC-1A cells after transfection with control or LARP1 siRNA. DAPI was used as a counter stain. Scale bar = 50 µm.

Journal: bioRxiv

Article Title: “Targeting LARP1 Enhances Carboplatin Sensitivity and Suppresses Tumor Growth in Endometrial Cancer”

doi: 10.64898/2026.03.22.713473

Figure Lengend Snippet: (A, B) Immunoblot analysis showing the protein expression of LARP1 and E2F1 after transfecting ISHI (A) and HEC-1A (B) cells with control or LARP1 siRNA. β-actin was used as a loading control. (C) Images representing immunofluorescence staining of LARP1 (green) and E2F1 (red) in HEC-1A cells after transfection with control or LARP1 siRNA. DAPI was used as a counter stain. Scale bar = 50 µm.

Article Snippet: Human endometrial cancer cell lines HEC-1A and Ishikawa (ISHI) were obtained from ATCC (American Type Culture Collection, Manassas, VA, USA).

Techniques: Western Blot, Expressing, Control, Immunofluorescence, Staining, Transfection

(A, B) MTS cell viability assay showing the effect of escalating concentration of carboplatin on the viability of ISHI (A) and HEC-1A cell lines. Sterile water was used as a vehicle control. One-way analysis of variance (ANOVA) was used to assess statistical significance among groups, followed by Tukey’s post hoc test for multiple pairwise comparisons. Data are presented as mean ± SD, with individual data points shown as dots.

Journal: bioRxiv

Article Title: “Targeting LARP1 Enhances Carboplatin Sensitivity and Suppresses Tumor Growth in Endometrial Cancer”

doi: 10.64898/2026.03.22.713473

Figure Lengend Snippet: (A, B) MTS cell viability assay showing the effect of escalating concentration of carboplatin on the viability of ISHI (A) and HEC-1A cell lines. Sterile water was used as a vehicle control. One-way analysis of variance (ANOVA) was used to assess statistical significance among groups, followed by Tukey’s post hoc test for multiple pairwise comparisons. Data are presented as mean ± SD, with individual data points shown as dots.

Article Snippet: Human endometrial cancer cell lines HEC-1A and Ishikawa (ISHI) were obtained from ATCC (American Type Culture Collection, Manassas, VA, USA).

Techniques: Viability Assay, Concentration Assay, Sterility, Control

(A, C) Micrographs representing colony formation assay showing the effect of LARP1 transfection and carboplatin treatment on the clonogenic survival of ISHI (A) and HEC-1A (B) cell lines. (B, D) Quantitative analysis of clonogenic assay showing the effect of LARP1 knockdown and carboplatin treatment on the clonogenic survival of ISHI (B) and HEC-1A (D) cells. One-way analysis of variance (ANOVA) was used to assess statistical significance among groups, followed by Tukey’s post hoc test for multiple pairwise comparisons. Data are presented as mean ± SD, with individual data points shown as dots.

Journal: bioRxiv

Article Title: “Targeting LARP1 Enhances Carboplatin Sensitivity and Suppresses Tumor Growth in Endometrial Cancer”

doi: 10.64898/2026.03.22.713473

Figure Lengend Snippet: (A, C) Micrographs representing colony formation assay showing the effect of LARP1 transfection and carboplatin treatment on the clonogenic survival of ISHI (A) and HEC-1A (B) cell lines. (B, D) Quantitative analysis of clonogenic assay showing the effect of LARP1 knockdown and carboplatin treatment on the clonogenic survival of ISHI (B) and HEC-1A (D) cells. One-way analysis of variance (ANOVA) was used to assess statistical significance among groups, followed by Tukey’s post hoc test for multiple pairwise comparisons. Data are presented as mean ± SD, with individual data points shown as dots.

Article Snippet: Human endometrial cancer cell lines HEC-1A and Ishikawa (ISHI) were obtained from ATCC (American Type Culture Collection, Manassas, VA, USA).

Techniques: Colony Assay, Transfection, Clonogenic Assay, Knockdown

GATA6 is a target gene of miR-196b. A : The nucleotide sequence of the miR-196b target site in GATA6 3′-UTR. Mut: contains a four-base mutation at the miR-196b target region. B : Double-luciferase reporter assays were used to detect the binding of miR-196b to the 3’-UTR region of GATA6. C - F : qPCR and WB were used to test the mRNA ( C , D ) and protein ( E , F ) levels of GATA6 in Ishikawa ( C , E ) and HEC-1 A ( D , F ) cells. (* P < 0.05, ** P < 0.01)

Journal: Biology Direct

Article Title: miR-196b inhibits tumor proliferation and metastasis by targeting GATA6 in endometrial cancer

doi: 10.1186/s13062-026-00749-9

Figure Lengend Snippet: GATA6 is a target gene of miR-196b. A : The nucleotide sequence of the miR-196b target site in GATA6 3′-UTR. Mut: contains a four-base mutation at the miR-196b target region. B : Double-luciferase reporter assays were used to detect the binding of miR-196b to the 3’-UTR region of GATA6. C - F : qPCR and WB were used to test the mRNA ( C , D ) and protein ( E , F ) levels of GATA6 in Ishikawa ( C , E ) and HEC-1 A ( D , F ) cells. (* P < 0.05, ** P < 0.01)

Article Snippet: Ishikawa and HEC-1 A cells (ATCC, USA) were cultured in RPMI-1640 or McCoy’s 5 A (Invitrogen, USA) medium supplemented with 10% fetal bovine serum (FBS; GIBCO, USA).

Techniques: Sequencing, Mutagenesis, Luciferase, Binding Assay

miR-196b reduces EC proliferation, migration, and invasion. A - B : qPCR detection of the expression of miR-196b in Ishikawa ( A ) and HEC-1 A ( B ) cells transfected with miR-196b mimics ( a ) or anti-miR-196b ( b ). C - D : CCK-8 assays were carried out to evaluate the proliferation capacity of Ishikawa ( C ) and HEC-1 A ( D ) cells transfected with miR-196b mimics ( a ) or anti-miR-196b ( b ). E-F: Colony formation assays were utilized to assess cell growth in Ishikawa ( E ) and HEC-1 A ( F ) cells. G - H : Wound healing assays were used to measure the migration ability in Ishikawa ( G ) and HEC-1 A ( H ) cells. I - J : Transwell assays were conducted to determine the invasion ability in Ishikawa ( I ) and HEC-1 A ( J ) cells. (Scale bar: 100 μm, * P < 0.05, ** P < 0.01)

Journal: Biology Direct

Article Title: miR-196b inhibits tumor proliferation and metastasis by targeting GATA6 in endometrial cancer

doi: 10.1186/s13062-026-00749-9

Figure Lengend Snippet: miR-196b reduces EC proliferation, migration, and invasion. A - B : qPCR detection of the expression of miR-196b in Ishikawa ( A ) and HEC-1 A ( B ) cells transfected with miR-196b mimics ( a ) or anti-miR-196b ( b ). C - D : CCK-8 assays were carried out to evaluate the proliferation capacity of Ishikawa ( C ) and HEC-1 A ( D ) cells transfected with miR-196b mimics ( a ) or anti-miR-196b ( b ). E-F: Colony formation assays were utilized to assess cell growth in Ishikawa ( E ) and HEC-1 A ( F ) cells. G - H : Wound healing assays were used to measure the migration ability in Ishikawa ( G ) and HEC-1 A ( H ) cells. I - J : Transwell assays were conducted to determine the invasion ability in Ishikawa ( I ) and HEC-1 A ( J ) cells. (Scale bar: 100 μm, * P < 0.05, ** P < 0.01)

Article Snippet: Ishikawa and HEC-1 A cells (ATCC, USA) were cultured in RPMI-1640 or McCoy’s 5 A (Invitrogen, USA) medium supplemented with 10% fetal bovine serum (FBS; GIBCO, USA).

Techniques: Migration, Expressing, Transfection, CCK-8 Assay

Impact of miR-196b on the cell cycle. A and C : Flow cytometry was used to detect the cell cycle distribution in Ishikawa ( A ) and HEC-1 A ( C ) cells following miR-196b mimics ( a ) and anti-miR-196b ( b ) transfection. B and D : WB to detect cyclin-associated protein levels in Ishikawa ( B ) and HEC-1 A ( D ) cells following miR-196b mimics ( a ) and anti-miR-196b ( b ) transfection. (* P < 0.05, ** P < 0.01)

Journal: Biology Direct

Article Title: miR-196b inhibits tumor proliferation and metastasis by targeting GATA6 in endometrial cancer

doi: 10.1186/s13062-026-00749-9

Figure Lengend Snippet: Impact of miR-196b on the cell cycle. A and C : Flow cytometry was used to detect the cell cycle distribution in Ishikawa ( A ) and HEC-1 A ( C ) cells following miR-196b mimics ( a ) and anti-miR-196b ( b ) transfection. B and D : WB to detect cyclin-associated protein levels in Ishikawa ( B ) and HEC-1 A ( D ) cells following miR-196b mimics ( a ) and anti-miR-196b ( b ) transfection. (* P < 0.05, ** P < 0.01)

Article Snippet: Ishikawa and HEC-1 A cells (ATCC, USA) were cultured in RPMI-1640 or McCoy’s 5 A (Invitrogen, USA) medium supplemented with 10% fetal bovine serum (FBS; GIBCO, USA).

Techniques: Flow Cytometry, Transfection

miR-196b inhibits EC proliferation in vivo. A : Weight growth curve of nude mice injected with agomir-196b-treated HEC-1 A cells. B : Tumor xenografts ( a ), tumor growth curve ( b ), and tumor weight ( c ) of nude mice at the study’s conclusion. qPCR ( d ) and WB ( e ) were performed to test the expression of miR-196b ( d ) and GATA6 ( e ) in tumors. The H&E staining reveals the pathological characteristics of the tumors from nude mice ( f ). C : Expression of GATA6, VEGF, p-AKT, and p-ERK in tumors was tested by immunohistochemical. (Scale bar: 100 μm, * P < 0.05, ** P < 0.01, *** P < 0.001)

Journal: Biology Direct

Article Title: miR-196b inhibits tumor proliferation and metastasis by targeting GATA6 in endometrial cancer

doi: 10.1186/s13062-026-00749-9

Figure Lengend Snippet: miR-196b inhibits EC proliferation in vivo. A : Weight growth curve of nude mice injected with agomir-196b-treated HEC-1 A cells. B : Tumor xenografts ( a ), tumor growth curve ( b ), and tumor weight ( c ) of nude mice at the study’s conclusion. qPCR ( d ) and WB ( e ) were performed to test the expression of miR-196b ( d ) and GATA6 ( e ) in tumors. The H&E staining reveals the pathological characteristics of the tumors from nude mice ( f ). C : Expression of GATA6, VEGF, p-AKT, and p-ERK in tumors was tested by immunohistochemical. (Scale bar: 100 μm, * P < 0.05, ** P < 0.01, *** P < 0.001)

Article Snippet: Ishikawa and HEC-1 A cells (ATCC, USA) were cultured in RPMI-1640 or McCoy’s 5 A (Invitrogen, USA) medium supplemented with 10% fetal bovine serum (FBS; GIBCO, USA).

Techniques: In Vivo, Injection, Expressing, Staining, Immunohistochemical staining

The miR-196b/GATA6 axis regulates EC proliferation, migration, and invasion. A - F : CCK8 ( A , B ), clone formation ( C , D ), and EdU assays ( E , F ) were used to detect the proliferation capacity of Ishikawa ( A , C , E ) and HEC-1 A ( B , D , F ) cells transfected with anti-miR-196b, siGATA6, and anti-miR-196b+siGATA6. G - J : Wound healing ( G , H ) and Transwell ( I , J ) assays assessing cell migration and invasion in Ishikawa ( G , I ) and HEC-1 A ( H , J ) cells. (Scale bar: 100 μm, * P < 0.05, ** P < 0.01)

Journal: Biology Direct

Article Title: miR-196b inhibits tumor proliferation and metastasis by targeting GATA6 in endometrial cancer

doi: 10.1186/s13062-026-00749-9

Figure Lengend Snippet: The miR-196b/GATA6 axis regulates EC proliferation, migration, and invasion. A - F : CCK8 ( A , B ), clone formation ( C , D ), and EdU assays ( E , F ) were used to detect the proliferation capacity of Ishikawa ( A , C , E ) and HEC-1 A ( B , D , F ) cells transfected with anti-miR-196b, siGATA6, and anti-miR-196b+siGATA6. G - J : Wound healing ( G , H ) and Transwell ( I , J ) assays assessing cell migration and invasion in Ishikawa ( G , I ) and HEC-1 A ( H , J ) cells. (Scale bar: 100 μm, * P < 0.05, ** P < 0.01)

Article Snippet: Ishikawa and HEC-1 A cells (ATCC, USA) were cultured in RPMI-1640 or McCoy’s 5 A (Invitrogen, USA) medium supplemented with 10% fetal bovine serum (FBS; GIBCO, USA).

Techniques: Migration, Transfection

miR-196b regulates the EMT process and AKT/ERK signaling pathways via GATA6 in EC cells. A - B : IF staining of E-cadherin ( A ) and vimentin ( B ) in Ishikawa ( a ) and HEC-1 A ( b ) cells. C - D : WB analysis of EMT-related markers ( C ) and AKT and ERK pathway-related proteins ( D ) in Ishikawa ( a ) and HEC-1 A ( b ) cells. (Scale bar: 100 μm, * P < 0.05, ** P < 0.01, *** P < 0.001)

Journal: Biology Direct

Article Title: miR-196b inhibits tumor proliferation and metastasis by targeting GATA6 in endometrial cancer

doi: 10.1186/s13062-026-00749-9

Figure Lengend Snippet: miR-196b regulates the EMT process and AKT/ERK signaling pathways via GATA6 in EC cells. A - B : IF staining of E-cadherin ( A ) and vimentin ( B ) in Ishikawa ( a ) and HEC-1 A ( b ) cells. C - D : WB analysis of EMT-related markers ( C ) and AKT and ERK pathway-related proteins ( D ) in Ishikawa ( a ) and HEC-1 A ( b ) cells. (Scale bar: 100 μm, * P < 0.05, ** P < 0.01, *** P < 0.001)

Article Snippet: Ishikawa and HEC-1 A cells (ATCC, USA) were cultured in RPMI-1640 or McCoy’s 5 A (Invitrogen, USA) medium supplemented with 10% fetal bovine serum (FBS; GIBCO, USA).

Techniques: Protein-Protein interactions, Staining

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