bovine testicular hyaluronidase (Worthington Biochemical)

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Name:

Hyaluronidase

Description:

A partially purified dialyzed lyophilized powder

Catalog Number:

ls002591

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Source:

Bovine Testes

Cas Number:

3726.33.3

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Structured Review

Worthington Biochemical bovine testicular hyaluronidase
A partially purified dialyzed lyophilized powder
https://www.bioz.com/result/bovine testicular hyaluronidase/product/Worthington Biochemical
Average 99 stars, based on 3 article reviews
Price from $9.99 to $1999.99

bovine testicular hyaluronidase - by Bioz Stars, 2020-10

99/100 stars

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Images

Modification:

Article Title: Interplay between CCR7 and Notch1 axes promotes stemness in MMTV-PyMT mammary cancer cells
Article Snippet: .. Briefly, mouse mammary glands/tumors were dissected, manually dissociated and then digested in Dulbecco’s modified Eagle’s medium (DMEM, Gibco) supplemented with 1 mg/ml collagenase, 100U/ml hyaluronidase (both from Worthington) and 2% foetal calf serum for 3 h at 37 °C. .. Resultant organoids were digested a further 15 min with 6U/ml dispase (Gibco) and 12U/ml DNase I (Merck) at 37 °C.

Incubation:

Article Title: Roflumilast and aquaporin‐2 regulation in rat renal inner medullary collecting duct. Roflumilast and aquaporin‐2 regulation in rat renal inner medullary collecting duct
Article Snippet: .. Briefly, kidney inner medullas were dissected, minced, and digested by incubation at 37°C for 70–90 min in digestion solution (250 mmol/L sucrose, 10 mmol/L triethanolamine, pH 7.6) containing collagenase B (3 mg/mL; Roche, Indianapolis, IN) and hyaluronidase (1400 U/mL; Worthington, Lakewood, NJ). ..

Article Title: Presynaptic Expression of LRIT3 Transsynaptically Organizes the Postsynaptic Glutamate Signaling Complex Containing TRPM1
Article Snippet: .. To remove the residual vitreous, the dissected retinas were incubated for 10 min in Ringers containing collagenase (240U/ml, cat # LS005273) and hyaluronidase (7200U/mL, cat # LS002592) (Worthington Biochemicals, Lakewood, NJ). .. Dorsal and ventral pieces of retina were dissected and placed on the MEA (60MEA200/30Ti; Multi Channel Systems, Reutlingen, Germany).

Article Title: The ribbon-associated protein C-terminal-binding protein 1 is not essential for the structure and function of retinal ribbon synapses
Article Snippet: .. Briefly, retinas were dissected from eyecups in standard solution, (NaCl 135 mM, KCl 5 mM, CaCl2 0.5 mM, MgCl2 1 mM, HEPES 10 mM, pH 7.4), treated for 25 min with hyaluronidase (500 units/ml; Worthington Biochemical, Lakewood, NJ), and then transferred to cold saline containing 2.7 mM DL-cysteine (Sigma-Aldrich, St. Louis, MO), cut into small pieces, and incubated 25–30 min in saline containing DL-cysteine and 15–30 units/ml papain (Sigma) at room temperature. .. A piece of retina was triturated via a flame-polished Pasteur pipette, and dissociated cells were plated onto glass-bottomed dishes containing saline with CaCl2 increased to 2.5 mM for patch-clamp recording.

other:

Article Title: MT1-MMP and Type II Collagen Specify Skeletal Stem Cells and Their Bone and Cartilage Progeny
Article Snippet: Sections were deparaffinized, hydrated, and blocked for endogenous peroxidase with 3% H2 O2 in methanol, and antigen was retrieved by hyaluronidase treatment (Worthington, Lakewood, NJ, USA).

Article Title: Hyaluronan Synthesis Mediates the Fibrotic Response of Keratocytes to Transforming Growth Factor ? *
Article Snippet: Keratocytes in serum-free DME/F12 were stimulated with 10 ng/ml PDGF for 24 h. The cells were rinsed and treated for 15 min with 500 μg/ml testicular hyaluronidase (3,000 USP/NF units/mg of protein; Worthington) at 37 °C.

Article Title: A 3-D organoid kidney culture model engineered for high-throughput nephrotoxicity assays.
Article Snippet: Cell-cell and cell-matrix interactions control cell phenotypes and functions in vivo.

Flow Cytometry:

Article Title: SHP2 Inhibition Abrogates Adaptive Resistance to KRASG12C-Inhibition and Remodels the Tumor Microenvironment of KRAS-Mutant Tumors
Article Snippet: .. Flow Cytometry Tumors were minced, chopped, and digested in DMEM containing 2.0 mg/mL collagenase IV (GIBCO), 1.0 mg/mL hyaluronidase (Worthington), 0.1% soybean trypsin inhibitor, 50U/mL DNase I (STEMCELL Technologies) at 37°C for 1h. .. Single cell suspensions were obtained by passage through a strainer (70 µm), washed in FACS buffer (PBS with 5% FBS), incubated with LIVE/DEAD Fixable Zombie Yellow Fixable Viability Kit (Biolegend, 423104) for 30 min., blocked with anti-CD16/32 (Biolegend, clone 93) for 5 min. on ice, and then incubated with fluorophore-conjugated antibodies on ice for 45 min. For detection of intracellular markers, FOXP3 Fixation/Permeabilization Buffer Set (BioLegend) was used, according to the manufacturer’s instructions.