bovine testicular hyaluronidase  (Worthington Biochemical)


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    Name:
    Hyaluronidase
    Description:
    A partially purified dialyzed lyophilized powder
    Catalog Number:
    LS002591
    Price:
    Inquire
    Source:
    Bovine Testes
    Size:
    Bulk
    Cas Number:
    3726.33.3
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    Structured Review

    Worthington Biochemical bovine testicular hyaluronidase
    A partially purified dialyzed lyophilized powder
    https://www.bioz.com/result/bovine testicular hyaluronidase/product/Worthington Biochemical
    Average 96 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    bovine testicular hyaluronidase - by Bioz Stars, 2021-04
    96/100 stars

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    Related Articles

    other:

    Article Title: Identification of Molecular Determinants of Primary and Metastatic Tumor Re-Initiation in Breast Cancer
    Article Snippet: The mixture was washed with PBS, spun down, and re-suspended in 10mL of dissociation media comprised of a 1:1 mixture of DMEM:F12 supplemented with penicillin, streptomycin and fungizone, 1.25mg/mL Collagenase Type I (Worthington Biochemical) and 1mg/mL Hyaluronidase (Worthington Biochemical).

    Article Title: In Vivo Non Linear Optical (NLO) Imaging in Live Rabbit Eyes Using the Heidelberg Two-Photon Laser Ophthalmoscope
    Article Snippet: Corneas were placed into the centrifuge tubes (15–20 corneas per tube) containing 50 mg hyaluronidase ( Worthington Biochemical Co., Lakewood, NJ, USA), 200 mg collagenase (17018-037 Gibco, Invitrogen Co., Carlsbad, CA, USA) and antibiotics in DMEM medium (31600-034 Gibco, Invitrogen Co., Carlsbad, CA, USA).

    Article Title: Endothelial adhesion of synchronized gastric tumor cells changes during cell cycle transit and correlates with the expression level of CD44 splice variants
    Article Snippet: In a second set of experiments, HUVEC were treated with hyaluronidase (Worthington, Lakewood, USA) for 60 min (1 000 U/mL, 2 500 U/mL, or 5 000 U/mL), before MKN45 cells were added.

    Article Title: NF-?B Is the Transcription Factor for FGF-2 That Causes Endothelial Mesenchymal Transformation in Cornea
    Article Snippet: Briefly, the Descemet's membrane complex was treated with 0.2% collagenase and 0.05% hyaluronidase (Worthington Biochemical, Lakewood, NJ) for 90 minutes at 37°C.

    Incubation:

    Article Title: Human CD4+ T cells specific for Merkel cell polyomavirus localize to Merkel cell carcinomas and target a required oncogenic domain
    Article Snippet: Peripheral mononuclear cells (PBMCs) were obtained from heparinized blood from MCC patients and healthy donors with Lymphocyte Separation Medium (Corning, Tewksbury MA) and cryopreserved in freezing medium [50% human serum (Valley Biomedical, Winchester VA), 40% RPMI (ThermoFisher, Waltham MA), and 10% DMSO (ThermoFisher, Waltham MA)]. .. Fresh MCC tumor material and punch biopsies ( > 1 cm3 ) were processed into single-cell digests by mincing them into small pieces with sterile forceps and scissors, followed by incubation in 20 mL of digestion medium composed of RPMI plus 0.002g DNase (Worthington Biochemical, Lakewood NJ), 0.008g collagenase (Worthington Biochemical, Lakewood NJ), and 0.002 g hyaluronidase (Worthington Biochemical, Lakewood NJ) in a 10 cm dish at 37°C with frequent, gentle swirling. ..

    Article Title: Tolvaptan as a tool in renal physiology
    Article Snippet: IMCD suspensions were prepared as previously described with slight modification ( ). .. Kidney inner medullas were dissected, minced, and digested by incubation at 37°C for 70–90 min in digestion solution (250 mM sucrose, 10 mM triethanolamine, pH 7.6) containing collagenase B (3 mg/ml; Roche, Indianapolis, IN) and hyaluronidase (1,400 U/ml; Worthington, Lakewood, NJ). ..

    Centrifugation:

    Article Title: Human Corneal Endothelial Cells Employ Phosphorylation of p27Kip1 at Both Ser10 and Thr187 Sites for FGF-2-Mediated Cell Proliferation via PI 3-Kinase
    Article Snippet: The Descemet's membrane and endothelium complex was dissected in small pieces and then incubated overnight in reduced serum media (OptiMEM-I; Gibco-BRL) supplemented with 8% fetal bovine serum (FBS; Omega Scientific, Tarzana, CA) overnight to stabilize the cells before culture. .. After centrifugation, the Descemet's membrane and endothelium complex was treated with 0.2% collagenase type II and 0.05% hyaluronidase (Worthington Biochemical, Lakewood, NJ) for 90 minutes at 37°C. .. After centrifugation, the primary cells were resuspended in culture medium: reduced serum media (OptiMEM-I; Gibco-BRL) supplemented with 8% FBS, 5 ng/mL epidermal growth factor (Upstate Biotechnologies, Lake Placid, NY), 20 ng/mL nerve growth factor (Biomedical Technologies, Stoughton, MA), 100 μg/mL bovine pituitary extract (Biomedical Technologies), 20 μg/mL ascorbic acid (Sigma-Aldrich), 200 mg/mL calcium chloride, 0.08% chondroitin sulfate (Sigma-Aldrich), 50 μg/mL gentamicin, and antibiotic-antimycotic solution (Sigma-Aldrich) diluted 1:100 and then plated in precoated 24-well tissue culture plates with tissue culture reagent (FNC coating mix; Biological Research Faculty & Facility, Inc., Ijamsville, MD).

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  • 96
    Worthington Biochemical bovine testicular hyaluronidase
    Bovine Testicular Hyaluronidase, supplied by Worthington Biochemical, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/bovine testicular hyaluronidase/product/Worthington Biochemical
    Average 96 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    bovine testicular hyaluronidase - by Bioz Stars, 2021-04
    96/100 stars
      Buy from Supplier

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