anti sp c  (Hycult Biotech)


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    Hycult Biotech anti sp c
    (A–C) Rfx2 expression in vehicle-treated, nintedanib-treated, and age-matched lung tissue. (A) Quantitative reverse transcription polymerase chain reaction analysis showed that Rfx2 expression was significantly downregulated in vehicle-treated mouse lung (n = 3) compared with that in age-matched D1CC×D1BB mouse lung (n = 3), and that this downregulation tended to be attenuated by nintedanib treatment (n = 3). The vehicle- and nintedanib-treated mouse lungs were obtained from the mice used in the analysis shown in . (B, C) Western blot analysis confirmed the findings shown in (A): age-matched D1CC×D1BB mouse lung, n = 4; vehicle-treated mouse lung, n = 4; nintedanib-treated mouse lung, n = 4 Relative signal intensity was determined using α Tubulin as the loading control. Data are presented as mean ± standard error. *, p < 0.05 by Dunnett’s test. (D) RNA in situ hybridization for Rfx2 revealed that Rfx2 was expressed strongly and weakly in cells adjacent to Scgb1a1 -positive cells and in some type I and type II alveolar epithelial cells, respectively. Sftpc and Scgb1a1 transcripts were used as an RNA-expression marker for type II alveolar cells and airway secreting cells, respectively. Scale bars = 20 μm. (E) Immunohistochemical staining revealed that RFX2 (red) was localized mainly in bronchial epithelium and somewhat in alveolar epithelium. The expressions <t>of</t> <t>E-cadherin</t> (green) and <t>SP-C</t> (white) were used as a marker for epithelial cells, and epithelial cells and type II alveolar epithelial cells, respectively. Scale bars = 20 μm.
    Anti Sp C, supplied by Hycult Biotech, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti sp c/product/Hycult Biotech
    Average 90 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    anti sp c - by Bioz Stars, 2024-10
    90/100 stars

    Images

    1) Product Images from "Nintedanib induces gene expression changes in the lung of induced-rheumatoid arthritis–associated interstitial lung disease mice"

    Article Title: Nintedanib induces gene expression changes in the lung of induced-rheumatoid arthritis–associated interstitial lung disease mice

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0270056

    (A–C) Rfx2 expression in vehicle-treated, nintedanib-treated, and age-matched lung tissue. (A) Quantitative reverse transcription polymerase chain reaction analysis showed that Rfx2 expression was significantly downregulated in vehicle-treated mouse lung (n = 3) compared with that in age-matched D1CC×D1BB mouse lung (n = 3), and that this downregulation tended to be attenuated by nintedanib treatment (n = 3). The vehicle- and nintedanib-treated mouse lungs were obtained from the mice used in the analysis shown in . (B, C) Western blot analysis confirmed the findings shown in (A): age-matched D1CC×D1BB mouse lung, n = 4; vehicle-treated mouse lung, n = 4; nintedanib-treated mouse lung, n = 4 Relative signal intensity was determined using α Tubulin as the loading control. Data are presented as mean ± standard error. *, p < 0.05 by Dunnett’s test. (D) RNA in situ hybridization for Rfx2 revealed that Rfx2 was expressed strongly and weakly in cells adjacent to Scgb1a1 -positive cells and in some type I and type II alveolar epithelial cells, respectively. Sftpc and Scgb1a1 transcripts were used as an RNA-expression marker for type II alveolar cells and airway secreting cells, respectively. Scale bars = 20 μm. (E) Immunohistochemical staining revealed that RFX2 (red) was localized mainly in bronchial epithelium and somewhat in alveolar epithelium. The expressions of E-cadherin (green) and SP-C (white) were used as a marker for epithelial cells, and epithelial cells and type II alveolar epithelial cells, respectively. Scale bars = 20 μm.
    Figure Legend Snippet: (A–C) Rfx2 expression in vehicle-treated, nintedanib-treated, and age-matched lung tissue. (A) Quantitative reverse transcription polymerase chain reaction analysis showed that Rfx2 expression was significantly downregulated in vehicle-treated mouse lung (n = 3) compared with that in age-matched D1CC×D1BB mouse lung (n = 3), and that this downregulation tended to be attenuated by nintedanib treatment (n = 3). The vehicle- and nintedanib-treated mouse lungs were obtained from the mice used in the analysis shown in . (B, C) Western blot analysis confirmed the findings shown in (A): age-matched D1CC×D1BB mouse lung, n = 4; vehicle-treated mouse lung, n = 4; nintedanib-treated mouse lung, n = 4 Relative signal intensity was determined using α Tubulin as the loading control. Data are presented as mean ± standard error. *, p < 0.05 by Dunnett’s test. (D) RNA in situ hybridization for Rfx2 revealed that Rfx2 was expressed strongly and weakly in cells adjacent to Scgb1a1 -positive cells and in some type I and type II alveolar epithelial cells, respectively. Sftpc and Scgb1a1 transcripts were used as an RNA-expression marker for type II alveolar cells and airway secreting cells, respectively. Scale bars = 20 μm. (E) Immunohistochemical staining revealed that RFX2 (red) was localized mainly in bronchial epithelium and somewhat in alveolar epithelium. The expressions of E-cadherin (green) and SP-C (white) were used as a marker for epithelial cells, and epithelial cells and type II alveolar epithelial cells, respectively. Scale bars = 20 μm.

    Techniques Used: Expressing, Reverse Transcription Polymerase Chain Reaction, Western Blot, RNA In Situ Hybridization, RNA Expression, Marker, Immunohistochemical staining, Staining

    anti sp c  (Hycult Biotech)


    Bioz Verified Symbol Hycult Biotech is a verified supplier
    Bioz Manufacturer Symbol Hycult Biotech manufactures this product  
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    Hycult Biotech anti sp c
    (A–C) Rfx2 expression in vehicle-treated, nintedanib-treated, and age-matched lung tissue. (A) Quantitative reverse transcription polymerase chain reaction analysis showed that Rfx2 expression was significantly downregulated in vehicle-treated mouse lung (n = 3) compared with that in age-matched D1CC×D1BB mouse lung (n = 3), and that this downregulation tended to be attenuated by nintedanib treatment (n = 3). The vehicle- and nintedanib-treated mouse lungs were obtained from the mice used in the analysis shown in . (B, C) Western blot analysis confirmed the findings shown in (A): age-matched D1CC×D1BB mouse lung, n = 4; vehicle-treated mouse lung, n = 4; nintedanib-treated mouse lung, n = 4 Relative signal intensity was determined using α Tubulin as the loading control. Data are presented as mean ± standard error. *, p < 0.05 by Dunnett’s test. (D) RNA in situ hybridization for Rfx2 revealed that Rfx2 was expressed strongly and weakly in cells adjacent to Scgb1a1 -positive cells and in some type I and type II alveolar epithelial cells, respectively. Sftpc and Scgb1a1 transcripts were used as an RNA-expression marker for type II alveolar cells and airway secreting cells, respectively. Scale bars = 20 μm. (E) Immunohistochemical staining revealed that RFX2 (red) was localized mainly in bronchial epithelium and somewhat in alveolar epithelium. The expressions <t>of</t> <t>E-cadherin</t> (green) and <t>SP-C</t> (white) were used as a marker for epithelial cells, and epithelial cells and type II alveolar epithelial cells, respectively. Scale bars = 20 μm.
    Anti Sp C, supplied by Hycult Biotech, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti sp c/product/Hycult Biotech
    Average 90 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    anti sp c - by Bioz Stars, 2024-10
    90/100 stars

    Images

    1) Product Images from "Nintedanib induces gene expression changes in the lung of induced-rheumatoid arthritis–associated interstitial lung disease mice"

    Article Title: Nintedanib induces gene expression changes in the lung of induced-rheumatoid arthritis–associated interstitial lung disease mice

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0270056

    (A–C) Rfx2 expression in vehicle-treated, nintedanib-treated, and age-matched lung tissue. (A) Quantitative reverse transcription polymerase chain reaction analysis showed that Rfx2 expression was significantly downregulated in vehicle-treated mouse lung (n = 3) compared with that in age-matched D1CC×D1BB mouse lung (n = 3), and that this downregulation tended to be attenuated by nintedanib treatment (n = 3). The vehicle- and nintedanib-treated mouse lungs were obtained from the mice used in the analysis shown in . (B, C) Western blot analysis confirmed the findings shown in (A): age-matched D1CC×D1BB mouse lung, n = 4; vehicle-treated mouse lung, n = 4; nintedanib-treated mouse lung, n = 4 Relative signal intensity was determined using α Tubulin as the loading control. Data are presented as mean ± standard error. *, p < 0.05 by Dunnett’s test. (D) RNA in situ hybridization for Rfx2 revealed that Rfx2 was expressed strongly and weakly in cells adjacent to Scgb1a1 -positive cells and in some type I and type II alveolar epithelial cells, respectively. Sftpc and Scgb1a1 transcripts were used as an RNA-expression marker for type II alveolar cells and airway secreting cells, respectively. Scale bars = 20 μm. (E) Immunohistochemical staining revealed that RFX2 (red) was localized mainly in bronchial epithelium and somewhat in alveolar epithelium. The expressions of E-cadherin (green) and SP-C (white) were used as a marker for epithelial cells, and epithelial cells and type II alveolar epithelial cells, respectively. Scale bars = 20 μm.
    Figure Legend Snippet: (A–C) Rfx2 expression in vehicle-treated, nintedanib-treated, and age-matched lung tissue. (A) Quantitative reverse transcription polymerase chain reaction analysis showed that Rfx2 expression was significantly downregulated in vehicle-treated mouse lung (n = 3) compared with that in age-matched D1CC×D1BB mouse lung (n = 3), and that this downregulation tended to be attenuated by nintedanib treatment (n = 3). The vehicle- and nintedanib-treated mouse lungs were obtained from the mice used in the analysis shown in . (B, C) Western blot analysis confirmed the findings shown in (A): age-matched D1CC×D1BB mouse lung, n = 4; vehicle-treated mouse lung, n = 4; nintedanib-treated mouse lung, n = 4 Relative signal intensity was determined using α Tubulin as the loading control. Data are presented as mean ± standard error. *, p < 0.05 by Dunnett’s test. (D) RNA in situ hybridization for Rfx2 revealed that Rfx2 was expressed strongly and weakly in cells adjacent to Scgb1a1 -positive cells and in some type I and type II alveolar epithelial cells, respectively. Sftpc and Scgb1a1 transcripts were used as an RNA-expression marker for type II alveolar cells and airway secreting cells, respectively. Scale bars = 20 μm. (E) Immunohistochemical staining revealed that RFX2 (red) was localized mainly in bronchial epithelium and somewhat in alveolar epithelium. The expressions of E-cadherin (green) and SP-C (white) were used as a marker for epithelial cells, and epithelial cells and type II alveolar epithelial cells, respectively. Scale bars = 20 μm.

    Techniques Used: Expressing, Reverse Transcription Polymerase Chain Reaction, Western Blot, RNA In Situ Hybridization, RNA Expression, Marker, Immunohistochemical staining, Staining

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    Hycult Biotech anti sp c
    (A–C) Rfx2 expression in vehicle-treated, nintedanib-treated, and age-matched lung tissue. (A) Quantitative reverse transcription polymerase chain reaction analysis showed that Rfx2 expression was significantly downregulated in vehicle-treated mouse lung (n = 3) compared with that in age-matched D1CC×D1BB mouse lung (n = 3), and that this downregulation tended to be attenuated by nintedanib treatment (n = 3). The vehicle- and nintedanib-treated mouse lungs were obtained from the mice used in the analysis shown in . (B, C) Western blot analysis confirmed the findings shown in (A): age-matched D1CC×D1BB mouse lung, n = 4; vehicle-treated mouse lung, n = 4; nintedanib-treated mouse lung, n = 4 Relative signal intensity was determined using α Tubulin as the loading control. Data are presented as mean ± standard error. *, p < 0.05 by Dunnett’s test. (D) RNA in situ hybridization for Rfx2 revealed that Rfx2 was expressed strongly and weakly in cells adjacent to Scgb1a1 -positive cells and in some type I and type II alveolar epithelial cells, respectively. Sftpc and Scgb1a1 transcripts were used as an RNA-expression marker for type II alveolar cells and airway secreting cells, respectively. Scale bars = 20 μm. (E) Immunohistochemical staining revealed that RFX2 (red) was localized mainly in bronchial epithelium and somewhat in alveolar epithelium. The expressions <t>of</t> <t>E-cadherin</t> (green) and <t>SP-C</t> (white) were used as a marker for epithelial cells, and epithelial cells and type II alveolar epithelial cells, respectively. Scale bars = 20 μm.
    Anti Sp C, supplied by Hycult Biotech, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti sp c/product/Hycult Biotech
    Average 90 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    anti sp c - by Bioz Stars, 2024-10
    90/100 stars
    		  Buy from Supplier

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    (A–C) Rfx2 expression in vehicle-treated, nintedanib-treated, and age-matched lung tissue. (A) Quantitative reverse transcription polymerase chain reaction analysis showed that Rfx2 expression was significantly downregulated in vehicle-treated mouse lung (n = 3) compared with that in age-matched D1CC×D1BB mouse lung (n = 3), and that this downregulation tended to be attenuated by nintedanib treatment (n = 3). The vehicle- and nintedanib-treated mouse lungs were obtained from the mice used in the analysis shown in . (B, C) Western blot analysis confirmed the findings shown in (A): age-matched D1CC×D1BB mouse lung, n = 4; vehicle-treated mouse lung, n = 4; nintedanib-treated mouse lung, n = 4 Relative signal intensity was determined using α Tubulin as the loading control. Data are presented as mean ± standard error. *, p < 0.05 by Dunnett’s test. (D) RNA in situ hybridization for Rfx2 revealed that Rfx2 was expressed strongly and weakly in cells adjacent to Scgb1a1 -positive cells and in some type I and type II alveolar epithelial cells, respectively. Sftpc and Scgb1a1 transcripts were used as an RNA-expression marker for type II alveolar cells and airway secreting cells, respectively. Scale bars = 20 μm. (E) Immunohistochemical staining revealed that RFX2 (red) was localized mainly in bronchial epithelium and somewhat in alveolar epithelium. The expressions of E-cadherin (green) and SP-C (white) were used as a marker for epithelial cells, and epithelial cells and type II alveolar epithelial cells, respectively. Scale bars = 20 μm.

    Journal: PLoS ONE

    Article Title: Nintedanib induces gene expression changes in the lung of induced-rheumatoid arthritis–associated interstitial lung disease mice

    doi: 10.1371/journal.pone.0270056

    Figure Lengend Snippet: (A–C) Rfx2 expression in vehicle-treated, nintedanib-treated, and age-matched lung tissue. (A) Quantitative reverse transcription polymerase chain reaction analysis showed that Rfx2 expression was significantly downregulated in vehicle-treated mouse lung (n = 3) compared with that in age-matched D1CC×D1BB mouse lung (n = 3), and that this downregulation tended to be attenuated by nintedanib treatment (n = 3). The vehicle- and nintedanib-treated mouse lungs were obtained from the mice used in the analysis shown in . (B, C) Western blot analysis confirmed the findings shown in (A): age-matched D1CC×D1BB mouse lung, n = 4; vehicle-treated mouse lung, n = 4; nintedanib-treated mouse lung, n = 4 Relative signal intensity was determined using α Tubulin as the loading control. Data are presented as mean ± standard error. *, p < 0.05 by Dunnett’s test. (D) RNA in situ hybridization for Rfx2 revealed that Rfx2 was expressed strongly and weakly in cells adjacent to Scgb1a1 -positive cells and in some type I and type II alveolar epithelial cells, respectively. Sftpc and Scgb1a1 transcripts were used as an RNA-expression marker for type II alveolar cells and airway secreting cells, respectively. Scale bars = 20 μm. (E) Immunohistochemical staining revealed that RFX2 (red) was localized mainly in bronchial epithelium and somewhat in alveolar epithelium. The expressions of E-cadherin (green) and SP-C (white) were used as a marker for epithelial cells, and epithelial cells and type II alveolar epithelial cells, respectively. Scale bars = 20 μm.

    Article Snippet: For multiplex staining of RFX2 in lung tissue, 2-μm-thick paraffin sections of lungs were reacted with the following primary antibodies: rabbit anti–E-cadherin (#3195, Cell Signaling Technology, MA, USA), rabbit anti–SP-C (#HP9050, Hycult Biotech, Netherlands), and rabbit anti-RFX2 (#12622-1-AP, Proteintech).

    Techniques: Expressing, Reverse Transcription Polymerase Chain Reaction, Western Blot, RNA In Situ Hybridization, RNA Expression, Marker, Immunohistochemical staining, Staining