Review



guinea pig anti occludin  (Hycult Biotech)


Bioz Verified Symbol Hycult Biotech is a verified supplier
Bioz Manufacturer Symbol Hycult Biotech manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
    • 90
      Buy from Supplier

    Structured Review

    Hycult Biotech guinea pig anti occludin
    (A/B/C) Caco-2 monolayers were differentiated for 21 days and then treated or not with 100 μg/ml HA35 for 24 hours, followed by challenge with or without 40 mM ethanol for 3 hours. (A) Cells were then fixed in methanol and immunoreactive ZO-1 (green) and <t>occludin</t> (red) visualized by confocal microscopy. Images acquired at 63X with a 3X digital magnification and are representative of at least two images per slide from three independent experiments. (B) Occludin and ZO-1 expression was assessed by immunoblot <t>using</t> <t>ß-actin</t> as a loading control. Semi-quantification of protein expression was performed using Carestream Imaging Software. (C) FITC-dextran (4Kd) was added to the upper chamber of the transwells and leakage into the lower chamber assessed at 1 and 2h. (D) Differentiated Caco-2 monolayers were treated with increasing concentrations of HA35 for 24 h and then challenged with 500 mM ethanol. TEER was measured immediately before the addition of ethanol and 3 h later. Values are expressed as means ±SEM, (A) n=3, (B) n=4–6 and (C) n=12 (values with different superscripts are significantly different within a time point, p<0.05) and (D) n=4 (*p < 0.05 compared to cells not treated with HA35)
    Guinea Pig Anti Occludin, supplied by Hycult Biotech, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/guinea pig anti occludin/product/Hycult Biotech
    Average 90 stars, based on 1 article reviews
    guinea pig anti occludin - by Bioz Stars, 2025-04
    90/100 stars

    Images

    1) Product Images from "Specifically-sized hyaluronan (35 kDa) prevents ethanol-induced disruption of epithelial tight junctions through a Layilin-dependent mechanism in Caco-2 cells"

    Article Title: Specifically-sized hyaluronan (35 kDa) prevents ethanol-induced disruption of epithelial tight junctions through a Layilin-dependent mechanism in Caco-2 cells

    Journal: Alcoholism, clinical and experimental research

    doi: 10.1111/acer.14140

    (A/B/C) Caco-2 monolayers were differentiated for 21 days and then treated or not with 100 μg/ml HA35 for 24 hours, followed by challenge with or without 40 mM ethanol for 3 hours. (A) Cells were then fixed in methanol and immunoreactive ZO-1 (green) and occludin (red) visualized by confocal microscopy. Images acquired at 63X with a 3X digital magnification and are representative of at least two images per slide from three independent experiments. (B) Occludin and ZO-1 expression was assessed by immunoblot using ß-actin as a loading control. Semi-quantification of protein expression was performed using Carestream Imaging Software. (C) FITC-dextran (4Kd) was added to the upper chamber of the transwells and leakage into the lower chamber assessed at 1 and 2h. (D) Differentiated Caco-2 monolayers were treated with increasing concentrations of HA35 for 24 h and then challenged with 500 mM ethanol. TEER was measured immediately before the addition of ethanol and 3 h later. Values are expressed as means ±SEM, (A) n=3, (B) n=4–6 and (C) n=12 (values with different superscripts are significantly different within a time point, p<0.05) and (D) n=4 (*p < 0.05 compared to cells not treated with HA35)
    Figure Legend Snippet: (A/B/C) Caco-2 monolayers were differentiated for 21 days and then treated or not with 100 μg/ml HA35 for 24 hours, followed by challenge with or without 40 mM ethanol for 3 hours. (A) Cells were then fixed in methanol and immunoreactive ZO-1 (green) and occludin (red) visualized by confocal microscopy. Images acquired at 63X with a 3X digital magnification and are representative of at least two images per slide from three independent experiments. (B) Occludin and ZO-1 expression was assessed by immunoblot using ß-actin as a loading control. Semi-quantification of protein expression was performed using Carestream Imaging Software. (C) FITC-dextran (4Kd) was added to the upper chamber of the transwells and leakage into the lower chamber assessed at 1 and 2h. (D) Differentiated Caco-2 monolayers were treated with increasing concentrations of HA35 for 24 h and then challenged with 500 mM ethanol. TEER was measured immediately before the addition of ethanol and 3 h later. Values are expressed as means ±SEM, (A) n=3, (B) n=4–6 and (C) n=12 (values with different superscripts are significantly different within a time point, p<0.05) and (D) n=4 (*p < 0.05 compared to cells not treated with HA35)

    Techniques Used: Confocal Microscopy, Expressing, Western Blot, Imaging, Software

    Caco-2 monolayers were differentiated on collagen-coated transwell filters and then transfected with scrambled siRNA or siRNA targeting Layilin. (A) Immunofluorescent staining for Layilin 24 h following transfection with scrambled siRNA or siRNA targeting Layilin. Images are representative of at least two images per slide from three independent transfections. (B) 24 h after transfection, cells were treated or not with 100μg/ml HA35 for 24 hours and then challenged with or without 40mM ethanol for 3 hours. Immunoreactive ZO-1 (green) and occludin (red) was visualized by confocal microscopy in cells transfected with scrambled siRNA or siRNA targeting Layilin. Images acquired at 63X with a 3X digital magnification and are representative of at least two images per slide from four independent experiments. (C) Cells transfected with scrambled siRNA or siRNA targeting Layilin were treated with 1 mg/ml HA35 for 24 h and then challenged with 500 mM ethanol. TEER was measured immediately after the addition of ethanol and 3 h later. Values are expressed as means ± SEM. Values are expressed as means ±SEM, (A) n=3, (B) n=6 and (C) n=4–6, *p < 0.05 compared to cells not treated with ethanol within a transfection group.
    Figure Legend Snippet: Caco-2 monolayers were differentiated on collagen-coated transwell filters and then transfected with scrambled siRNA or siRNA targeting Layilin. (A) Immunofluorescent staining for Layilin 24 h following transfection with scrambled siRNA or siRNA targeting Layilin. Images are representative of at least two images per slide from three independent transfections. (B) 24 h after transfection, cells were treated or not with 100μg/ml HA35 for 24 hours and then challenged with or without 40mM ethanol for 3 hours. Immunoreactive ZO-1 (green) and occludin (red) was visualized by confocal microscopy in cells transfected with scrambled siRNA or siRNA targeting Layilin. Images acquired at 63X with a 3X digital magnification and are representative of at least two images per slide from four independent experiments. (C) Cells transfected with scrambled siRNA or siRNA targeting Layilin were treated with 1 mg/ml HA35 for 24 h and then challenged with 500 mM ethanol. TEER was measured immediately after the addition of ethanol and 3 h later. Values are expressed as means ± SEM. Values are expressed as means ±SEM, (A) n=3, (B) n=6 and (C) n=4–6, *p < 0.05 compared to cells not treated with ethanol within a transfection group.

    Techniques Used: Transfection, Staining, Confocal Microscopy



    Similar Products

    guinea pig anti occludin  (Hycult Biotech)
    90
    Hycult Biotech guinea pig anti occludin
    (A/B/C) Caco-2 monolayers were differentiated for 21 days and then treated or not with 100 μg/ml HA35 for 24 hours, followed by challenge with or without 40 mM ethanol for 3 hours. (A) Cells were then fixed in methanol and immunoreactive ZO-1 (green) and <t>occludin</t> (red) visualized by confocal microscopy. Images acquired at 63X with a 3X digital magnification and are representative of at least two images per slide from three independent experiments. (B) Occludin and ZO-1 expression was assessed by immunoblot <t>using</t> <t>ß-actin</t> as a loading control. Semi-quantification of protein expression was performed using Carestream Imaging Software. (C) FITC-dextran (4Kd) was added to the upper chamber of the transwells and leakage into the lower chamber assessed at 1 and 2h. (D) Differentiated Caco-2 monolayers were treated with increasing concentrations of HA35 for 24 h and then challenged with 500 mM ethanol. TEER was measured immediately before the addition of ethanol and 3 h later. Values are expressed as means ±SEM, (A) n=3, (B) n=4–6 and (C) n=12 (values with different superscripts are significantly different within a time point, p<0.05) and (D) n=4 (*p < 0.05 compared to cells not treated with HA35)
    Guinea Pig Anti Occludin, supplied by Hycult Biotech, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/guinea pig anti occludin/product/Hycult Biotech
    Average 90 stars, based on 1 article reviews
    guinea pig anti occludin - by Bioz Stars, 2025-04
    90/100 stars
    		  Buy from Supplier
    occludin, human, pab  (Hycult Biotech)
    90
    Hycult Biotech occludin, human, pab
    (A/B/C) Caco-2 monolayers were differentiated for 21 days and then treated or not with 100 μg/ml HA35 for 24 hours, followed by challenge with or without 40 mM ethanol for 3 hours. (A) Cells were then fixed in methanol and immunoreactive ZO-1 (green) and <t>occludin</t> (red) visualized by confocal microscopy. Images acquired at 63X with a 3X digital magnification and are representative of at least two images per slide from three independent experiments. (B) Occludin and ZO-1 expression was assessed by immunoblot <t>using</t> <t>ß-actin</t> as a loading control. Semi-quantification of protein expression was performed using Carestream Imaging Software. (C) FITC-dextran (4Kd) was added to the upper chamber of the transwells and leakage into the lower chamber assessed at 1 and 2h. (D) Differentiated Caco-2 monolayers were treated with increasing concentrations of HA35 for 24 h and then challenged with 500 mM ethanol. TEER was measured immediately before the addition of ethanol and 3 h later. Values are expressed as means ±SEM, (A) n=3, (B) n=4–6 and (C) n=12 (values with different superscripts are significantly different within a time point, p<0.05) and (D) n=4 (*p < 0.05 compared to cells not treated with HA35)
    Occludin, Human, Pab, supplied by Hycult Biotech, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/occludin, human, pab/product/Hycult Biotech
    Average 90 stars, based on 1 article reviews
    occludin, human, pab - by Bioz Stars, 2025-04
    90/100 stars
    		  Buy from Supplier

    Image Search Results


    (A/B/C) Caco-2 monolayers were differentiated for 21 days and then treated or not with 100 μg/ml HA35 for 24 hours, followed by challenge with or without 40 mM ethanol for 3 hours. (A) Cells were then fixed in methanol and immunoreactive ZO-1 (green) and occludin (red) visualized by confocal microscopy. Images acquired at 63X with a 3X digital magnification and are representative of at least two images per slide from three independent experiments. (B) Occludin and ZO-1 expression was assessed by immunoblot using ß-actin as a loading control. Semi-quantification of protein expression was performed using Carestream Imaging Software. (C) FITC-dextran (4Kd) was added to the upper chamber of the transwells and leakage into the lower chamber assessed at 1 and 2h. (D) Differentiated Caco-2 monolayers were treated with increasing concentrations of HA35 for 24 h and then challenged with 500 mM ethanol. TEER was measured immediately before the addition of ethanol and 3 h later. Values are expressed as means ±SEM, (A) n=3, (B) n=4–6 and (C) n=12 (values with different superscripts are significantly different within a time point, p<0.05) and (D) n=4 (*p < 0.05 compared to cells not treated with HA35)

    Journal: Alcoholism, clinical and experimental research

    Article Title: Specifically-sized hyaluronan (35 kDa) prevents ethanol-induced disruption of epithelial tight junctions through a Layilin-dependent mechanism in Caco-2 cells

    doi: 10.1111/acer.14140

    Figure Lengend Snippet: (A/B/C) Caco-2 monolayers were differentiated for 21 days and then treated or not with 100 μg/ml HA35 for 24 hours, followed by challenge with or without 40 mM ethanol for 3 hours. (A) Cells were then fixed in methanol and immunoreactive ZO-1 (green) and occludin (red) visualized by confocal microscopy. Images acquired at 63X with a 3X digital magnification and are representative of at least two images per slide from three independent experiments. (B) Occludin and ZO-1 expression was assessed by immunoblot using ß-actin as a loading control. Semi-quantification of protein expression was performed using Carestream Imaging Software. (C) FITC-dextran (4Kd) was added to the upper chamber of the transwells and leakage into the lower chamber assessed at 1 and 2h. (D) Differentiated Caco-2 monolayers were treated with increasing concentrations of HA35 for 24 h and then challenged with 500 mM ethanol. TEER was measured immediately before the addition of ethanol and 3 h later. Values are expressed as means ±SEM, (A) n=3, (B) n=4–6 and (C) n=12 (values with different superscripts are significantly different within a time point, p<0.05) and (D) n=4 (*p < 0.05 compared to cells not treated with HA35)

    Article Snippet: Primary antibodies were purchased from the following sources: rabbit anti-ZO-1 (Life Technologies, 61–7300), guinea pig anti-occludin (Hycult HP9047), rabbit anti-ß-actin (Cell Signaling, 4967), rabbit anti-Layilin (Bioss, 1842R).

    Techniques: Confocal Microscopy, Expressing, Western Blot, Imaging, Software

    Caco-2 monolayers were differentiated on collagen-coated transwell filters and then transfected with scrambled siRNA or siRNA targeting Layilin. (A) Immunofluorescent staining for Layilin 24 h following transfection with scrambled siRNA or siRNA targeting Layilin. Images are representative of at least two images per slide from three independent transfections. (B) 24 h after transfection, cells were treated or not with 100μg/ml HA35 for 24 hours and then challenged with or without 40mM ethanol for 3 hours. Immunoreactive ZO-1 (green) and occludin (red) was visualized by confocal microscopy in cells transfected with scrambled siRNA or siRNA targeting Layilin. Images acquired at 63X with a 3X digital magnification and are representative of at least two images per slide from four independent experiments. (C) Cells transfected with scrambled siRNA or siRNA targeting Layilin were treated with 1 mg/ml HA35 for 24 h and then challenged with 500 mM ethanol. TEER was measured immediately after the addition of ethanol and 3 h later. Values are expressed as means ± SEM. Values are expressed as means ±SEM, (A) n=3, (B) n=6 and (C) n=4–6, *p < 0.05 compared to cells not treated with ethanol within a transfection group.

    Journal: Alcoholism, clinical and experimental research

    Article Title: Specifically-sized hyaluronan (35 kDa) prevents ethanol-induced disruption of epithelial tight junctions through a Layilin-dependent mechanism in Caco-2 cells

    doi: 10.1111/acer.14140

    Figure Lengend Snippet: Caco-2 monolayers were differentiated on collagen-coated transwell filters and then transfected with scrambled siRNA or siRNA targeting Layilin. (A) Immunofluorescent staining for Layilin 24 h following transfection with scrambled siRNA or siRNA targeting Layilin. Images are representative of at least two images per slide from three independent transfections. (B) 24 h after transfection, cells were treated or not with 100μg/ml HA35 for 24 hours and then challenged with or without 40mM ethanol for 3 hours. Immunoreactive ZO-1 (green) and occludin (red) was visualized by confocal microscopy in cells transfected with scrambled siRNA or siRNA targeting Layilin. Images acquired at 63X with a 3X digital magnification and are representative of at least two images per slide from four independent experiments. (C) Cells transfected with scrambled siRNA or siRNA targeting Layilin were treated with 1 mg/ml HA35 for 24 h and then challenged with 500 mM ethanol. TEER was measured immediately after the addition of ethanol and 3 h later. Values are expressed as means ± SEM. Values are expressed as means ±SEM, (A) n=3, (B) n=6 and (C) n=4–6, *p < 0.05 compared to cells not treated with ethanol within a transfection group.

    Article Snippet: Primary antibodies were purchased from the following sources: rabbit anti-ZO-1 (Life Technologies, 61–7300), guinea pig anti-occludin (Hycult HP9047), rabbit anti-ß-actin (Cell Signaling, 4967), rabbit anti-Layilin (Bioss, 1842R).

    Techniques: Transfection, Staining, Confocal Microscopy