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anti rat c3  (Hycult Biotech)


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    Structured Review

    Hycult Biotech anti rat c3
    Anti Rat C3, supplied by Hycult Biotech, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti rat c3/product/Hycult Biotech
    Average 93 stars, based on 1 article reviews
    anti rat c3 - by Bioz Stars, 2025-02
    93/100 stars

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    Hycult Biotech total c3
    (A) Scanning laser ophthalmoscope image of the retinae: Large clusters of hyperfluorescent foci (showing as autofluorescent point sources are observed. (B–D) Immunohistochemically labelled sections of the fluorescent debris/deposits, the majority of which may be containined within macrophages, often occur simultaneously on either side of the RPE. (B) acts as a negative control for Aβ detection by IHC (background signal from an isotype specific control antibody). The signal for fluorescent debris, (yellow), was dampened by use of Sudan Black in all cases. RPE-associated foci, show cross-reactivity, (green, highlighted with white arrows), to activated complement <t>C3,</t> (C3b+, detected with clone 2/11, <t>#HM1065,</t> <t>Hycult</t> Biotech, ), (C), and Aβ, (4G8+), (D). A- scalebar = 200 µm; B–D scalebar = 25 µm. Blue label is DAPI, (4', 6-diamidino-2-phenylindole), a nuclear stain. CHO = choroid, RPE = retinal pigment epithelial cells.
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    Image Search Results


    Primary antibodies used for IHC detection in cfh−/− mouse retinae.

    Journal: PLoS ONE

    Article Title: Systemic Administration of Abeta mAb Reduces Retinal Deposition of Abeta and Activated Complement C3 in Age-Related Macular Degeneration Mouse Model

    doi: 10.1371/journal.pone.0065518

    Figure Lengend Snippet: Primary antibodies used for IHC detection in cfh−/− mouse retinae.

    Article Snippet: Rabbit anti-rat polyclonal Ab to total C3 , Hycult Biotechnology , HP8022 , 1∶50.

    Techniques:

    Log scored means +/− SE for Amyloid β (4G8+, blue) and complement C3 (C3+, red, detected with rabbit anti-rat polyclonal Ab to total C3, Hycult, HP80222, ), deposition in retinal sections after 3 months of dosing with: (i) 6F6, [solid bars]; (ii) IgG, IgG2A isotype specific negative control Ab, [open bars], at 600 µg weekly doses. Group sizes, n = numbers of eyes treated, are marked at the base of the bars in parentheses. Differences of **p = 0.0232, for Amyloid β deposition and of *p = 0.0414 for C3 deposition were noted comparing 6F6 treated with IgG2A control. See SI .

    Journal: PLoS ONE

    Article Title: Systemic Administration of Abeta mAb Reduces Retinal Deposition of Abeta and Activated Complement C3 in Age-Related Macular Degeneration Mouse Model

    doi: 10.1371/journal.pone.0065518

    Figure Lengend Snippet: Log scored means +/− SE for Amyloid β (4G8+, blue) and complement C3 (C3+, red, detected with rabbit anti-rat polyclonal Ab to total C3, Hycult, HP80222, ), deposition in retinal sections after 3 months of dosing with: (i) 6F6, [solid bars]; (ii) IgG, IgG2A isotype specific negative control Ab, [open bars], at 600 µg weekly doses. Group sizes, n = numbers of eyes treated, are marked at the base of the bars in parentheses. Differences of **p = 0.0232, for Amyloid β deposition and of *p = 0.0414 for C3 deposition were noted comparing 6F6 treated with IgG2A control. See SI .

    Article Snippet: Rabbit anti-rat polyclonal Ab to total C3 , Hycult Biotechnology , HP8022 , 1∶50.

    Techniques: Negative Control

    Primary antibodies used for IHC detection in cfh−/− mouse retinae.

    Journal: PLoS ONE

    Article Title: Systemic Administration of Abeta mAb Reduces Retinal Deposition of Abeta and Activated Complement C3 in Age-Related Macular Degeneration Mouse Model

    doi: 10.1371/journal.pone.0065518

    Figure Lengend Snippet: Primary antibodies used for IHC detection in cfh−/− mouse retinae.

    Article Snippet: Note that in this study, total C3, (ie. both activated and uncleaved C3), was being detected using a rabbit polyclonal anti-Rat C3 antibody, (Hycult biotech, cat# HP8022, ), and yet statistical significance for co-clearance of Aβ and complement C3 was still demonstrated by IHC.

    Techniques:

    Log scored means +/− SE for Amyloid β (4G8+, blue) and complement C3 (C3+, red, detected with rabbit anti-rat polyclonal Ab to total C3, Hycult, HP80222, ), deposition in retinal sections after 3 months of dosing with: (i) 6F6, [solid bars]; (ii) IgG, IgG2A isotype specific negative control Ab, [open bars], at 600 µg weekly doses. Group sizes, n = numbers of eyes treated, are marked at the base of the bars in parentheses. Differences of **p = 0.0232, for Amyloid β deposition and of *p = 0.0414 for C3 deposition were noted comparing 6F6 treated with IgG2A control. See SI .

    Journal: PLoS ONE

    Article Title: Systemic Administration of Abeta mAb Reduces Retinal Deposition of Abeta and Activated Complement C3 in Age-Related Macular Degeneration Mouse Model

    doi: 10.1371/journal.pone.0065518

    Figure Lengend Snippet: Log scored means +/− SE for Amyloid β (4G8+, blue) and complement C3 (C3+, red, detected with rabbit anti-rat polyclonal Ab to total C3, Hycult, HP80222, ), deposition in retinal sections after 3 months of dosing with: (i) 6F6, [solid bars]; (ii) IgG, IgG2A isotype specific negative control Ab, [open bars], at 600 µg weekly doses. Group sizes, n = numbers of eyes treated, are marked at the base of the bars in parentheses. Differences of **p = 0.0232, for Amyloid β deposition and of *p = 0.0414 for C3 deposition were noted comparing 6F6 treated with IgG2A control. See SI .

    Article Snippet: Note that in this study, total C3, (ie. both activated and uncleaved C3), was being detected using a rabbit polyclonal anti-Rat C3 antibody, (Hycult biotech, cat# HP8022, ), and yet statistical significance for co-clearance of Aβ and complement C3 was still demonstrated by IHC.

    Techniques: Negative Control

    Primary antibodies used for IHC detection in cfh−/− mouse retinae.

    Journal: PLoS ONE

    Article Title: Systemic Administration of Abeta mAb Reduces Retinal Deposition of Abeta and Activated Complement C3 in Age-Related Macular Degeneration Mouse Model

    doi: 10.1371/journal.pone.0065518

    Figure Lengend Snippet: Primary antibodies used for IHC detection in cfh−/− mouse retinae.

    Article Snippet: Rabbit anti-rat polyclonal Ab to total C3 , Hycult Biotechnology , HP8022 , 1∶50.

    Techniques:

    (A) Scanning laser ophthalmoscope image of the retinae: Large clusters of hyperfluorescent foci (showing as autofluorescent point sources are observed. (B–D) Immunohistochemically labelled sections of the fluorescent debris/deposits, the majority of which may be containined within macrophages, often occur simultaneously on either side of the RPE. (B) acts as a negative control for Aβ detection by IHC (background signal from an isotype specific control antibody). The signal for fluorescent debris, (yellow), was dampened by use of Sudan Black in all cases. RPE-associated foci, show cross-reactivity, (green, highlighted with white arrows), to activated complement C3, (C3b+, detected with clone 2/11, #HM1065, Hycult Biotech, ), (C), and Aβ, (4G8+), (D). A- scalebar = 200 µm; B–D scalebar = 25 µm. Blue label is DAPI, (4', 6-diamidino-2-phenylindole), a nuclear stain. CHO = choroid, RPE = retinal pigment epithelial cells.

    Journal: PLoS ONE

    Article Title: Systemic Administration of Abeta mAb Reduces Retinal Deposition of Abeta and Activated Complement C3 in Age-Related Macular Degeneration Mouse Model

    doi: 10.1371/journal.pone.0065518

    Figure Lengend Snippet: (A) Scanning laser ophthalmoscope image of the retinae: Large clusters of hyperfluorescent foci (showing as autofluorescent point sources are observed. (B–D) Immunohistochemically labelled sections of the fluorescent debris/deposits, the majority of which may be containined within macrophages, often occur simultaneously on either side of the RPE. (B) acts as a negative control for Aβ detection by IHC (background signal from an isotype specific control antibody). The signal for fluorescent debris, (yellow), was dampened by use of Sudan Black in all cases. RPE-associated foci, show cross-reactivity, (green, highlighted with white arrows), to activated complement C3, (C3b+, detected with clone 2/11, #HM1065, Hycult Biotech, ), (C), and Aβ, (4G8+), (D). A- scalebar = 200 µm; B–D scalebar = 25 µm. Blue label is DAPI, (4', 6-diamidino-2-phenylindole), a nuclear stain. CHO = choroid, RPE = retinal pigment epithelial cells.

    Article Snippet: Although the same primary antibody was used for the detection of Aβ deposition as in the remainder of the study, (4G8, ), this was biotin-labelled and detected with Alexa Fluor 488 streptavidin, and a different primary antibody was used to detect total C3, (Hycult biotech, cat# HP8022) a rabbit polyclonal anti-Rat C3 antibody which gave low background for IHC staining, (data from this study is described solely in the final part of the Results section and was the only occasion that the antibody combination described above was used in this work).

    Techniques: Negative Control, Staining

    Primary antibodies used for IHC detection in cfh−/− mouse retinae.

    Journal: PLoS ONE

    Article Title: Systemic Administration of Abeta mAb Reduces Retinal Deposition of Abeta and Activated Complement C3 in Age-Related Macular Degeneration Mouse Model

    doi: 10.1371/journal.pone.0065518

    Figure Lengend Snippet: Primary antibodies used for IHC detection in cfh−/− mouse retinae.

    Article Snippet: Although the same primary antibody was used for the detection of Aβ deposition as in the remainder of the study, (4G8, ), this was biotin-labelled and detected with Alexa Fluor 488 streptavidin, and a different primary antibody was used to detect total C3, (Hycult biotech, cat# HP8022) a rabbit polyclonal anti-Rat C3 antibody which gave low background for IHC staining, (data from this study is described solely in the final part of the Results section and was the only occasion that the antibody combination described above was used in this work).

    Techniques:

    Aβ (4G8+, red) and activated complement C3 (detected with clone 2/11+, #HM1065, Hycult Biotech., , green), cross reactivity to retinal sections at 6 months, (after 3 months dosing): (A) negative control (PBS treated), 6F6, at 60 µg dose (B) and 600 µg dose (C), scalebar = 25 µm, PR = phototreceptors/outer segments, RPE = retinal pigment epithelium, CHO = choroid, DAPI = 4',6-diamidino-2-phenylindole. Left hand panel (A), (B), (C) is stained with DAPI, right hand panel (A), (B), (C), is without DAPI staining. Aβ deposition (D) and activated complement C3 deposition (E) in the RPE/Bruch’s membrane and/or along the outer side of the choriocapillaris, (B), of cfh−/− mice are plotted as differences from vehicle control with 95% confidence intervals (CI), after prophylactic treatment in the test groups. Mice dosed with 6F6 show a dose dependent significant reduction, (asterisks, see text for levels of significance), in Aβ deposition at 60 µg, 300 µg and 600 µg and in activated C3 deposition at 300 µg and 600 µg compared to those treated with vehicle, (D) and (E) respectively.

    Journal: PLoS ONE

    Article Title: Systemic Administration of Abeta mAb Reduces Retinal Deposition of Abeta and Activated Complement C3 in Age-Related Macular Degeneration Mouse Model

    doi: 10.1371/journal.pone.0065518

    Figure Lengend Snippet: Aβ (4G8+, red) and activated complement C3 (detected with clone 2/11+, #HM1065, Hycult Biotech., , green), cross reactivity to retinal sections at 6 months, (after 3 months dosing): (A) negative control (PBS treated), 6F6, at 60 µg dose (B) and 600 µg dose (C), scalebar = 25 µm, PR = phototreceptors/outer segments, RPE = retinal pigment epithelium, CHO = choroid, DAPI = 4',6-diamidino-2-phenylindole. Left hand panel (A), (B), (C) is stained with DAPI, right hand panel (A), (B), (C), is without DAPI staining. Aβ deposition (D) and activated complement C3 deposition (E) in the RPE/Bruch’s membrane and/or along the outer side of the choriocapillaris, (B), of cfh−/− mice are plotted as differences from vehicle control with 95% confidence intervals (CI), after prophylactic treatment in the test groups. Mice dosed with 6F6 show a dose dependent significant reduction, (asterisks, see text for levels of significance), in Aβ deposition at 60 µg, 300 µg and 600 µg and in activated C3 deposition at 300 µg and 600 µg compared to those treated with vehicle, (D) and (E) respectively.

    Article Snippet: Although the same primary antibody was used for the detection of Aβ deposition as in the remainder of the study, (4G8, ), this was biotin-labelled and detected with Alexa Fluor 488 streptavidin, and a different primary antibody was used to detect total C3, (Hycult biotech, cat# HP8022) a rabbit polyclonal anti-Rat C3 antibody which gave low background for IHC staining, (data from this study is described solely in the final part of the Results section and was the only occasion that the antibody combination described above was used in this work).

    Techniques: Negative Control, Staining

    Aβ (4G8+, red) and activated complement C3 (detected with clone 2/11+, #HM1065, Hycult Biotech., , green), cross reactivity to retinal sections at 9 months, (after 3 months dosing): (A) negative control (PBS treated), 6F6, at 60 µg dose (B) and 600 µg dose (C). Scalebar = 25 µm), PR = phototreceptors/outer segments, RPE = retinal pigment epithelium, CHO = choroid, DAPI = 4',6-diamidino-2-phenylindole. Left hand panel (A), (B), (C) is stained with DAPI, right hand panel (A), (B), (C), is without DAPI staining. Aβ (D), and activated complement C3, (E), deposition in the RPE/Bruch’s membrane of cfh−/− mice, are plotted as differences from vehicle control with 95% confidence intervals (CI) after therapeutic treatment. Data in graphs (D) and (E) show data at an intermediate time point, after 7 months of age (7MO), 1 month (+4w) after treatment as well as at the end of the regime at 9 months of age (9MO), 3 months (+12w) after treatment. Data in graphs (D) and (E) also shows wild type (WT) mice treated with 6F6 (WT6F6) after 7 months of age (7MO), 1 month (+4w) after treatment. Mice dosed with 6F6 show a dose dependent significant reduction, (asterisks, see text for levels of significance), in Aβ deposition at 60 µg and 600 µg and in activated C3 deposition at 600 µg compared compared to those treated with vehicle, (D) at 9 months of age (9MO), 3 months (+12w) after treatment.

    Journal: PLoS ONE

    Article Title: Systemic Administration of Abeta mAb Reduces Retinal Deposition of Abeta and Activated Complement C3 in Age-Related Macular Degeneration Mouse Model

    doi: 10.1371/journal.pone.0065518

    Figure Lengend Snippet: Aβ (4G8+, red) and activated complement C3 (detected with clone 2/11+, #HM1065, Hycult Biotech., , green), cross reactivity to retinal sections at 9 months, (after 3 months dosing): (A) negative control (PBS treated), 6F6, at 60 µg dose (B) and 600 µg dose (C). Scalebar = 25 µm), PR = phototreceptors/outer segments, RPE = retinal pigment epithelium, CHO = choroid, DAPI = 4',6-diamidino-2-phenylindole. Left hand panel (A), (B), (C) is stained with DAPI, right hand panel (A), (B), (C), is without DAPI staining. Aβ (D), and activated complement C3, (E), deposition in the RPE/Bruch’s membrane of cfh−/− mice, are plotted as differences from vehicle control with 95% confidence intervals (CI) after therapeutic treatment. Data in graphs (D) and (E) show data at an intermediate time point, after 7 months of age (7MO), 1 month (+4w) after treatment as well as at the end of the regime at 9 months of age (9MO), 3 months (+12w) after treatment. Data in graphs (D) and (E) also shows wild type (WT) mice treated with 6F6 (WT6F6) after 7 months of age (7MO), 1 month (+4w) after treatment. Mice dosed with 6F6 show a dose dependent significant reduction, (asterisks, see text for levels of significance), in Aβ deposition at 60 µg and 600 µg and in activated C3 deposition at 600 µg compared compared to those treated with vehicle, (D) at 9 months of age (9MO), 3 months (+12w) after treatment.

    Article Snippet: Although the same primary antibody was used for the detection of Aβ deposition as in the remainder of the study, (4G8, ), this was biotin-labelled and detected with Alexa Fluor 488 streptavidin, and a different primary antibody was used to detect total C3, (Hycult biotech, cat# HP8022) a rabbit polyclonal anti-Rat C3 antibody which gave low background for IHC staining, (data from this study is described solely in the final part of the Results section and was the only occasion that the antibody combination described above was used in this work).

    Techniques: Negative Control, Staining

    Log scored means +/− SE for Amyloid β (4G8+, blue) and complement C3 (C3+, red, detected with rabbit anti-rat polyclonal Ab to total C3, Hycult, HP80222, ), deposition in retinal sections after 3 months of dosing with: (i) 6F6, [solid bars]; (ii) IgG, IgG2A isotype specific negative control Ab, [open bars], at 600 µg weekly doses. Group sizes, n = numbers of eyes treated, are marked at the base of the bars in parentheses. Differences of **p = 0.0232, for Amyloid β deposition and of *p = 0.0414 for C3 deposition were noted comparing 6F6 treated with IgG2A control. See SI .

    Journal: PLoS ONE

    Article Title: Systemic Administration of Abeta mAb Reduces Retinal Deposition of Abeta and Activated Complement C3 in Age-Related Macular Degeneration Mouse Model

    doi: 10.1371/journal.pone.0065518

    Figure Lengend Snippet: Log scored means +/− SE for Amyloid β (4G8+, blue) and complement C3 (C3+, red, detected with rabbit anti-rat polyclonal Ab to total C3, Hycult, HP80222, ), deposition in retinal sections after 3 months of dosing with: (i) 6F6, [solid bars]; (ii) IgG, IgG2A isotype specific negative control Ab, [open bars], at 600 µg weekly doses. Group sizes, n = numbers of eyes treated, are marked at the base of the bars in parentheses. Differences of **p = 0.0232, for Amyloid β deposition and of *p = 0.0414 for C3 deposition were noted comparing 6F6 treated with IgG2A control. See SI .

    Article Snippet: Although the same primary antibody was used for the detection of Aβ deposition as in the remainder of the study, (4G8, ), this was biotin-labelled and detected with Alexa Fluor 488 streptavidin, and a different primary antibody was used to detect total C3, (Hycult biotech, cat# HP8022) a rabbit polyclonal anti-Rat C3 antibody which gave low background for IHC staining, (data from this study is described solely in the final part of the Results section and was the only occasion that the antibody combination described above was used in this work).

    Techniques: Negative Control