c3 cleavage  (Hycult Biotech)


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    Structured Review

    Hycult Biotech c3 cleavage
    Activation of complement in adipose tissue during ethanol feeding to mice. A, quantity of immunoreactive C3d in adipose tissue lysates was measured by Western blot analysis in wild-type mice fed ethanol diets for up to 25 days or pair-fed control diets for 25 days. HSC70 was used as a loading control. Values represent the means ± S.E., n = 4–8. *, p < 0.02 compared with pair-fed. B, quantity of immunoreactive C3d in plasma was measured by Western blot from pair- or ethanol-fed wild-type mice at 18 and 25 days. Immunoreactive C3d is expressed relative to the quantity of plasma loaded onto the gels. Values represent the means ± S.E., n = 8–10. *, p < 0.01, compared with pair-fed. C and D, <t>immunoreactive</t> <t>C3b</t> (red) was detected by immunohistochemistry in paraffin-embedded section of adipose from pair (P)- and ethanol (E)-fed wild-type (C) or cyp2e1−/− or Bid−/− mice (D). DAPI (blue) was used to visualize nuclei. Chow-fed <t>C3−/−</t> mice (C) were used as a negative control. Images are representative of at least four mice per diet group.
    C3 Cleavage, supplied by Hycult Biotech, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/c3 cleavage/product/Hycult Biotech
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    c3 cleavage - by Bioz Stars, 2024-02
    94/100 stars

    Images

    1) Product Images from "Identification of a Cytochrome P4502E1/Bid/C1q-dependent Axis Mediating Inflammation in Adipose Tissue after Chronic Ethanol Feeding to Mice"

    Article Title: Identification of a Cytochrome P4502E1/Bid/C1q-dependent Axis Mediating Inflammation in Adipose Tissue after Chronic Ethanol Feeding to Mice

    Journal: The Journal of Biological Chemistry

    doi: 10.1074/jbc.M111.254201

    Activation of complement in adipose tissue during ethanol feeding to mice. A, quantity of immunoreactive C3d in adipose tissue lysates was measured by Western blot analysis in wild-type mice fed ethanol diets for up to 25 days or pair-fed control diets for 25 days. HSC70 was used as a loading control. Values represent the means ± S.E., n = 4–8. *, p < 0.02 compared with pair-fed. B, quantity of immunoreactive C3d in plasma was measured by Western blot from pair- or ethanol-fed wild-type mice at 18 and 25 days. Immunoreactive C3d is expressed relative to the quantity of plasma loaded onto the gels. Values represent the means ± S.E., n = 8–10. *, p < 0.01, compared with pair-fed. C and D, immunoreactive C3b (red) was detected by immunohistochemistry in paraffin-embedded section of adipose from pair (P)- and ethanol (E)-fed wild-type (C) or cyp2e1−/− or Bid−/− mice (D). DAPI (blue) was used to visualize nuclei. Chow-fed C3−/− mice (C) were used as a negative control. Images are representative of at least four mice per diet group.
    Figure Legend Snippet: Activation of complement in adipose tissue during ethanol feeding to mice. A, quantity of immunoreactive C3d in adipose tissue lysates was measured by Western blot analysis in wild-type mice fed ethanol diets for up to 25 days or pair-fed control diets for 25 days. HSC70 was used as a loading control. Values represent the means ± S.E., n = 4–8. *, p < 0.02 compared with pair-fed. B, quantity of immunoreactive C3d in plasma was measured by Western blot from pair- or ethanol-fed wild-type mice at 18 and 25 days. Immunoreactive C3d is expressed relative to the quantity of plasma loaded onto the gels. Values represent the means ± S.E., n = 8–10. *, p < 0.01, compared with pair-fed. C and D, immunoreactive C3b (red) was detected by immunohistochemistry in paraffin-embedded section of adipose from pair (P)- and ethanol (E)-fed wild-type (C) or cyp2e1−/− or Bid−/− mice (D). DAPI (blue) was used to visualize nuclei. Chow-fed C3−/− mice (C) were used as a negative control. Images are representative of at least four mice per diet group.

    Techniques Used: Activation Assay, Western Blot, Immunohistochemistry, Negative Control

    c3 cleavage  (Hycult Biotech)


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  • 94

    Structured Review

    Hycult Biotech c3 cleavage
    Activation of complement in adipose tissue during ethanol feeding to mice. A, quantity of immunoreactive C3d in adipose tissue lysates was measured by Western blot analysis in wild-type mice fed ethanol diets for up to 25 days or pair-fed control diets for 25 days. HSC70 was used as a loading control. Values represent the means ± S.E., n = 4–8. *, p < 0.02 compared with pair-fed. B, quantity of immunoreactive C3d in plasma was measured by Western blot from pair- or ethanol-fed wild-type mice at 18 and 25 days. Immunoreactive C3d is expressed relative to the quantity of plasma loaded onto the gels. Values represent the means ± S.E., n = 8–10. *, p < 0.01, compared with pair-fed. C and D, <t>immunoreactive</t> <t>C3b</t> (red) was detected by immunohistochemistry in paraffin-embedded section of adipose from pair (P)- and ethanol (E)-fed wild-type (C) or cyp2e1−/− or Bid−/− mice (D). DAPI (blue) was used to visualize nuclei. Chow-fed <t>C3−/−</t> mice (C) were used as a negative control. Images are representative of at least four mice per diet group.
    C3 Cleavage, supplied by Hycult Biotech, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/c3 cleavage/product/Hycult Biotech
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    c3 cleavage - by Bioz Stars, 2024-02
    94/100 stars

    Images

    1) Product Images from "Identification of a Cytochrome P4502E1/Bid/C1q-dependent Axis Mediating Inflammation in Adipose Tissue after Chronic Ethanol Feeding to Mice"

    Article Title: Identification of a Cytochrome P4502E1/Bid/C1q-dependent Axis Mediating Inflammation in Adipose Tissue after Chronic Ethanol Feeding to Mice

    Journal: The Journal of Biological Chemistry

    doi: 10.1074/jbc.M111.254201

    Activation of complement in adipose tissue during ethanol feeding to mice. A, quantity of immunoreactive C3d in adipose tissue lysates was measured by Western blot analysis in wild-type mice fed ethanol diets for up to 25 days or pair-fed control diets for 25 days. HSC70 was used as a loading control. Values represent the means ± S.E., n = 4–8. *, p < 0.02 compared with pair-fed. B, quantity of immunoreactive C3d in plasma was measured by Western blot from pair- or ethanol-fed wild-type mice at 18 and 25 days. Immunoreactive C3d is expressed relative to the quantity of plasma loaded onto the gels. Values represent the means ± S.E., n = 8–10. *, p < 0.01, compared with pair-fed. C and D, immunoreactive C3b (red) was detected by immunohistochemistry in paraffin-embedded section of adipose from pair (P)- and ethanol (E)-fed wild-type (C) or cyp2e1−/− or Bid−/− mice (D). DAPI (blue) was used to visualize nuclei. Chow-fed C3−/− mice (C) were used as a negative control. Images are representative of at least four mice per diet group.
    Figure Legend Snippet: Activation of complement in adipose tissue during ethanol feeding to mice. A, quantity of immunoreactive C3d in adipose tissue lysates was measured by Western blot analysis in wild-type mice fed ethanol diets for up to 25 days or pair-fed control diets for 25 days. HSC70 was used as a loading control. Values represent the means ± S.E., n = 4–8. *, p < 0.02 compared with pair-fed. B, quantity of immunoreactive C3d in plasma was measured by Western blot from pair- or ethanol-fed wild-type mice at 18 and 25 days. Immunoreactive C3d is expressed relative to the quantity of plasma loaded onto the gels. Values represent the means ± S.E., n = 8–10. *, p < 0.01, compared with pair-fed. C and D, immunoreactive C3b (red) was detected by immunohistochemistry in paraffin-embedded section of adipose from pair (P)- and ethanol (E)-fed wild-type (C) or cyp2e1−/− or Bid−/− mice (D). DAPI (blue) was used to visualize nuclei. Chow-fed C3−/− mice (C) were used as a negative control. Images are representative of at least four mice per diet group.

    Techniques Used: Activation Assay, Western Blot, Immunohistochemistry, Negative Control

    c3  (Hycult Biotech)


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    Hycult Biotech c3
    C3, supplied by Hycult Biotech, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/c3/product/Hycult Biotech
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    c3 - by Bioz Stars, 2024-02
    94/100 stars

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    Hycult Biotech c3 cleavage
    Activation of complement in adipose tissue during ethanol feeding to mice. A, quantity of immunoreactive C3d in adipose tissue lysates was measured by Western blot analysis in wild-type mice fed ethanol diets for up to 25 days or pair-fed control diets for 25 days. HSC70 was used as a loading control. Values represent the means ± S.E., n = 4–8. *, p < 0.02 compared with pair-fed. B, quantity of immunoreactive C3d in plasma was measured by Western blot from pair- or ethanol-fed wild-type mice at 18 and 25 days. Immunoreactive C3d is expressed relative to the quantity of plasma loaded onto the gels. Values represent the means ± S.E., n = 8–10. *, p < 0.01, compared with pair-fed. C and D, <t>immunoreactive</t> <t>C3b</t> (red) was detected by immunohistochemistry in paraffin-embedded section of adipose from pair (P)- and ethanol (E)-fed wild-type (C) or cyp2e1−/− or Bid−/− mice (D). DAPI (blue) was used to visualize nuclei. Chow-fed <t>C3−/−</t> mice (C) were used as a negative control. Images are representative of at least four mice per diet group.
    C3 Cleavage, supplied by Hycult Biotech, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/c3 cleavage/product/Hycult Biotech
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    c3 cleavage - by Bioz Stars, 2024-02
    94/100 stars
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    Hycult Biotech c3
    Activation of complement in adipose tissue during ethanol feeding to mice. A, quantity of immunoreactive C3d in adipose tissue lysates was measured by Western blot analysis in wild-type mice fed ethanol diets for up to 25 days or pair-fed control diets for 25 days. HSC70 was used as a loading control. Values represent the means ± S.E., n = 4–8. *, p < 0.02 compared with pair-fed. B, quantity of immunoreactive C3d in plasma was measured by Western blot from pair- or ethanol-fed wild-type mice at 18 and 25 days. Immunoreactive C3d is expressed relative to the quantity of plasma loaded onto the gels. Values represent the means ± S.E., n = 8–10. *, p < 0.01, compared with pair-fed. C and D, <t>immunoreactive</t> <t>C3b</t> (red) was detected by immunohistochemistry in paraffin-embedded section of adipose from pair (P)- and ethanol (E)-fed wild-type (C) or cyp2e1−/− or Bid−/− mice (D). DAPI (blue) was used to visualize nuclei. Chow-fed <t>C3−/−</t> mice (C) were used as a negative control. Images are representative of at least four mice per diet group.
    C3, supplied by Hycult Biotech, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/c3/product/Hycult Biotech
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    c3 - by Bioz Stars, 2024-02
    94/100 stars
      Buy from Supplier

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    Activation of complement in adipose tissue during ethanol feeding to mice. A, quantity of immunoreactive C3d in adipose tissue lysates was measured by Western blot analysis in wild-type mice fed ethanol diets for up to 25 days or pair-fed control diets for 25 days. HSC70 was used as a loading control. Values represent the means ± S.E., n = 4–8. *, p < 0.02 compared with pair-fed. B, quantity of immunoreactive C3d in plasma was measured by Western blot from pair- or ethanol-fed wild-type mice at 18 and 25 days. Immunoreactive C3d is expressed relative to the quantity of plasma loaded onto the gels. Values represent the means ± S.E., n = 8–10. *, p < 0.01, compared with pair-fed. C and D, immunoreactive C3b (red) was detected by immunohistochemistry in paraffin-embedded section of adipose from pair (P)- and ethanol (E)-fed wild-type (C) or cyp2e1−/− or Bid−/− mice (D). DAPI (blue) was used to visualize nuclei. Chow-fed C3−/− mice (C) were used as a negative control. Images are representative of at least four mice per diet group.

    Journal: The Journal of Biological Chemistry

    Article Title: Identification of a Cytochrome P4502E1/Bid/C1q-dependent Axis Mediating Inflammation in Adipose Tissue after Chronic Ethanol Feeding to Mice

    doi: 10.1074/jbc.M111.254201

    Figure Lengend Snippet: Activation of complement in adipose tissue during ethanol feeding to mice. A, quantity of immunoreactive C3d in adipose tissue lysates was measured by Western blot analysis in wild-type mice fed ethanol diets for up to 25 days or pair-fed control diets for 25 days. HSC70 was used as a loading control. Values represent the means ± S.E., n = 4–8. *, p < 0.02 compared with pair-fed. B, quantity of immunoreactive C3d in plasma was measured by Western blot from pair- or ethanol-fed wild-type mice at 18 and 25 days. Immunoreactive C3d is expressed relative to the quantity of plasma loaded onto the gels. Values represent the means ± S.E., n = 8–10. *, p < 0.01, compared with pair-fed. C and D, immunoreactive C3b (red) was detected by immunohistochemistry in paraffin-embedded section of adipose from pair (P)- and ethanol (E)-fed wild-type (C) or cyp2e1−/− or Bid−/− mice (D). DAPI (blue) was used to visualize nuclei. Chow-fed C3−/− mice (C) were used as a negative control. Images are representative of at least four mice per diet group.

    Article Snippet: Immunoreactive C3b/iC3b/C3c (collectively termed “C3b” here) was detected with an antibody against neo-epitopes revealed upon C3 cleavage (Hycult Biotech; Canton, MA), as described ( 29 ).

    Techniques: Activation Assay, Western Blot, Immunohistochemistry, Negative Control