Sybr Selected Mastermix 2×, supplied by Solis BioDyne, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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1) Product Images from "LPMO-oxidized cellulose oligosaccharides evoke immunity in Arabidopsis conferring resistance towards necrotrophic fungus B. cinerea"
Article Title: LPMO-oxidized cellulose oligosaccharides evoke immunity in Arabidopsis conferring resistance towards necrotrophic fungus B. cinerea
Journal: Communications Biology
Figure Legend Snippet: Involvement of selected RLK receptors in signaling the AA9_COS-dependent defense gene expression. The expression of defense marker genes was evaluated in sif2 , sif4 , bak1.3 , and the1.1 loss-of-function Arabidopsis seedlings. Fourteen-day-old seedlings were treated with or without 100 µM AA9_COS and qRT-PCR was carried out 1 h after treatment. Median values are plotted in the boxes with data generated from three independent pools ( n = 3) of 15 seedlings each. One-way ANOVA test was performed; lower case letters denote significant ( p
Techniques Used: Expressing, Marker, Quantitative RT-PCR, Generated
Figure Legend Snippet: Transcriptomic changes comparison between AA9_COS and cellobiose (CB) treatments. a Hierarchical clustering analysis of gene expression patterns that show significant expression changes as compared to mock (log 2 FC ≥ 1 or ≤−1, p ≤ 0.05) 1 h after 100 µM AA9_COS or 100 µM cellobiose treatments in 14-day-old Arabidopsis Col-0 plants. The clusters were divided into upregulated (yellow) or downregulated (blue) genes. b The proportion of genes associated with the defense response GO term (GO:0006952 Supplementary Data 3 ) was evaluated per cluster. c Heatmap of the selected defense genes belonging to clusters I–III. d Venn diagram shows specific and shared upregulated and downregulated genes induced by AA9_COS or cellobiose treatments. e The GO enrichment analyses performed with PSGEA indicated that defense- and phytohormone-related genes were upregulated both by AA9_COS and cellobiose treatments (Supplementary Data 4 ). The color key represents the enrichment significance shown as −log 2 (FDR).
Techniques Used: Expressing