hot firepol probe qpcr mix plus  (Solis BioDyne)


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    Solis BioDyne hot firepol probe qpcr mix plus
    Seasonal changes in the honey bee midgut/pyloric microbiota. A) Mean microbiota composition throughout a foraging season between May and October for the four dominating components found in the honey bee midgut/pylorus by <t>mixed</t> sequencing. The MCR-ALS score, determined by mixed sequencing, represents an approximately relative bacterial composition in the honey bee midgut/pylorus without assuming closure of the system. B) α-diversity between May and October calculated from the raw spectra of mixed sequencing. C) Mean bacterial load for each month between May and October. The calculated relative ratio between 16S rRNA genes and vitellogenin genes (bacteria/bee), in the midgut/pylorus determined by quantitative PCR, is shown. Significant differences were observed between May and October (one-way ANOVA p =
    Hot Firepol Probe Qpcr Mix Plus, supplied by Solis BioDyne, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    1) Product Images from "Shifts in the Midgut/Pyloric Microbiota Composition within a Honey Bee Apiary throughout a Season"

    Article Title: Shifts in the Midgut/Pyloric Microbiota Composition within a Honey Bee Apiary throughout a Season

    Journal: Microbes and Environments

    doi: 10.1264/jsme2.ME15019

    Seasonal changes in the honey bee midgut/pyloric microbiota. A) Mean microbiota composition throughout a foraging season between May and October for the four dominating components found in the honey bee midgut/pylorus by mixed sequencing. The MCR-ALS score, determined by mixed sequencing, represents an approximately relative bacterial composition in the honey bee midgut/pylorus without assuming closure of the system. B) α-diversity between May and October calculated from the raw spectra of mixed sequencing. C) Mean bacterial load for each month between May and October. The calculated relative ratio between 16S rRNA genes and vitellogenin genes (bacteria/bee), in the midgut/pylorus determined by quantitative PCR, is shown. Significant differences were observed between May and October (one-way ANOVA p =
    Figure Legend Snippet: Seasonal changes in the honey bee midgut/pyloric microbiota. A) Mean microbiota composition throughout a foraging season between May and October for the four dominating components found in the honey bee midgut/pylorus by mixed sequencing. The MCR-ALS score, determined by mixed sequencing, represents an approximately relative bacterial composition in the honey bee midgut/pylorus without assuming closure of the system. B) α-diversity between May and October calculated from the raw spectra of mixed sequencing. C) Mean bacterial load for each month between May and October. The calculated relative ratio between 16S rRNA genes and vitellogenin genes (bacteria/bee), in the midgut/pylorus determined by quantitative PCR, is shown. Significant differences were observed between May and October (one-way ANOVA p =

    Techniques Used: Sequencing, Real-time Polymerase Chain Reaction

    2) Product Images from "Novel Gallate Triphenylphosphonium Derivatives with Potent Antichagasic Activity"

    Article Title: Novel Gallate Triphenylphosphonium Derivatives with Potent Antichagasic Activity

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0136852

    Effect of TPP + derivatives on the parasite load in T . cruzi -infected cells. RAW 264.7 cells were infected with T . cruzi trypomastigotes (Y strain); the cells were treated for 48 hours with the different derivatives, and the parasite load was assessed by qPCR. The results are expressed as the relative quantification of the parasite DNA and mammalian DNA, with a ratio of 1 assigned to the control. Treated cells were compared with the control using the ∆∆ C T method. *: p
    Figure Legend Snippet: Effect of TPP + derivatives on the parasite load in T . cruzi -infected cells. RAW 264.7 cells were infected with T . cruzi trypomastigotes (Y strain); the cells were treated for 48 hours with the different derivatives, and the parasite load was assessed by qPCR. The results are expressed as the relative quantification of the parasite DNA and mammalian DNA, with a ratio of 1 assigned to the control. Treated cells were compared with the control using the ∆∆ C T method. *: p

    Techniques Used: Infection, Real-time Polymerase Chain Reaction

    3) Product Images from "The relationship between osteoclastogenic and anti-osteoclastogenic pro-inflammatory cytokines differs in human osteoporotic and osteoarthritic bone tissues"

    Article Title: The relationship between osteoclastogenic and anti-osteoclastogenic pro-inflammatory cytokines differs in human osteoporotic and osteoarthritic bone tissues

    Journal: Journal of Biomedical Science

    doi: 10.1186/1423-0127-19-28

    Quantitative real-time PCR data of the pro-inflammatory cytokine mRNA levels in osteoporosis (OP) and osteoarthritis (OA) . Ligand to receptor ratios for each of the studied cytokine ligand receptor pairs were calculated from mRNA values normalized to geometric mean of  GAPDH  and  RPLP0  mRNA. Values are medians. Comparisons were assessed by the Mann-Whitney  U  test, * p  values
    Figure Legend Snippet: Quantitative real-time PCR data of the pro-inflammatory cytokine mRNA levels in osteoporosis (OP) and osteoarthritis (OA) . Ligand to receptor ratios for each of the studied cytokine ligand receptor pairs were calculated from mRNA values normalized to geometric mean of GAPDH and RPLP0 mRNA. Values are medians. Comparisons were assessed by the Mann-Whitney U test, * p values

    Techniques Used: Real-time Polymerase Chain Reaction, MANN-WHITNEY

    4) Product Images from "The potential applications of fibrin-coated electrospun polylactide nanofibers in skin tissue engineering"

    Article Title: The potential applications of fibrin-coated electrospun polylactide nanofibers in skin tissue engineering

    Journal: International Journal of Nanomedicine

    doi: 10.2147/IJN.S99317

    Relative expression of β 1 -integrins in human dermal fibroblasts on days 7, 10, and 14 after seeding on nonmodified PLA membranes or on PLA membranes with a fibrin nanocoating (F) determined by real-time PCR. Notes:  The cells were cultivated in the standard cell culture medium or in the medium supplemented with AA. The PS served as a control material. Reference gene GAPDH. ANOVA, Student–Newman–Keuls method, statistical significance ( P ≤0.05): *in comparison with the nonmodified PLA membrane in the standard cell culture medium or in the medium with AA. Abbreviations:  PLA, polylactide; PCR, polymerase chain reaction; AA, 2-phospho- l -ascorbic acid trisodium salt; PS, polystyrene culture dish; GAPDH, glyceraldehyde 3-phosphate dehydrogenase; ANOVA, analysis of variance.
    Figure Legend Snippet: Relative expression of β 1 -integrins in human dermal fibroblasts on days 7, 10, and 14 after seeding on nonmodified PLA membranes or on PLA membranes with a fibrin nanocoating (F) determined by real-time PCR. Notes: The cells were cultivated in the standard cell culture medium or in the medium supplemented with AA. The PS served as a control material. Reference gene GAPDH. ANOVA, Student–Newman–Keuls method, statistical significance ( P ≤0.05): *in comparison with the nonmodified PLA membrane in the standard cell culture medium or in the medium with AA. Abbreviations: PLA, polylactide; PCR, polymerase chain reaction; AA, 2-phospho- l -ascorbic acid trisodium salt; PS, polystyrene culture dish; GAPDH, glyceraldehyde 3-phosphate dehydrogenase; ANOVA, analysis of variance.

    Techniques Used: Expressing, Proximity Ligation Assay, Real-time Polymerase Chain Reaction, Cell Culture, Polymerase Chain Reaction

    Relative expression of collagen I in human dermal fibroblasts on days 7, 10, and 14 after cell seeding on nonmodified PLA membranes or on PLA membranes with a fibrin nanocoating (F) determined by real-time PCR. Notes:  The cells were cultivated in the standard cell culture medium or in the medium supplemented with AA. The PS served as a control material. Reference gene GAPDH. ANOVA, Student–Newman–Keuls method, statistical significance ( P ≤0.05): *in comparison with the nonmodified PLA membrane in the standard cell culture medium or in the medium with AA, AA in comparison with membranes in the standard cell culture medium, and  # statistical significance between PLA + AA and PLA F on day 14. Abbreviations:  PLA, polylactide; PCR, polymerase chain reaction; AA, 2-phospho- l -ascorbic acid trisodium salt; PS, polystyrene culture dish; GAPDH, glyceraldehyde 3-phosphate dehydrogenase; ANOVA, analysis of variance.
    Figure Legend Snippet: Relative expression of collagen I in human dermal fibroblasts on days 7, 10, and 14 after cell seeding on nonmodified PLA membranes or on PLA membranes with a fibrin nanocoating (F) determined by real-time PCR. Notes: The cells were cultivated in the standard cell culture medium or in the medium supplemented with AA. The PS served as a control material. Reference gene GAPDH. ANOVA, Student–Newman–Keuls method, statistical significance ( P ≤0.05): *in comparison with the nonmodified PLA membrane in the standard cell culture medium or in the medium with AA, AA in comparison with membranes in the standard cell culture medium, and # statistical significance between PLA + AA and PLA F on day 14. Abbreviations: PLA, polylactide; PCR, polymerase chain reaction; AA, 2-phospho- l -ascorbic acid trisodium salt; PS, polystyrene culture dish; GAPDH, glyceraldehyde 3-phosphate dehydrogenase; ANOVA, analysis of variance.

    Techniques Used: Expressing, Proximity Ligation Assay, Real-time Polymerase Chain Reaction, Cell Culture, Polymerase Chain Reaction

    5) Product Images from "Optimization of an O2-balanced bioartificial pancreas for type 1 diabetes using statistical design of experiment"

    Article Title: Optimization of an O2-balanced bioartificial pancreas for type 1 diabetes using statistical design of experiment

    Journal: Scientific Reports

    doi: 10.1038/s41598-022-07887-w

    Hypoxic signature of neonate pig islets (NPIs) in O 2  balanced BAPs. Alginate encapsulated NPIs (3000 IEQ/150 µL) were cultured for 3, 8, and 15 days under 20% O 2  (20% O 2 , positive control, white bar) or 1% O 2  condition without O 2  strategy (1% O 2,  negative control, black bar) or with the innovative strategy of oxygenation (ISO) composed of silicone-CaO 2  disk and 500 µg/mL HEMOXCell by alginate (1% O 2  + ISO, grey bars). Fold change in VEGF secretion by total metabolic activity (AU/RLU) of encapsulated NPIs compared to the control cultured for 3 days under 20% O 2  without O 2  strategy. ( B ) Relative quantitative RT-PCR expression analysis of Heme oxygenase (HO-1) of decapsulated NPIs cultured for 15 days within BAPs (n = 4–7). Results from independent experiments (n = 4–7) are expressed  as mean ± SEM. *p 
    Figure Legend Snippet: Hypoxic signature of neonate pig islets (NPIs) in O 2 balanced BAPs. Alginate encapsulated NPIs (3000 IEQ/150 µL) were cultured for 3, 8, and 15 days under 20% O 2 (20% O 2 , positive control, white bar) or 1% O 2 condition without O 2 strategy (1% O 2, negative control, black bar) or with the innovative strategy of oxygenation (ISO) composed of silicone-CaO 2 disk and 500 µg/mL HEMOXCell by alginate (1% O 2  + ISO, grey bars). Fold change in VEGF secretion by total metabolic activity (AU/RLU) of encapsulated NPIs compared to the control cultured for 3 days under 20% O 2 without O 2 strategy. ( B ) Relative quantitative RT-PCR expression analysis of Heme oxygenase (HO-1) of decapsulated NPIs cultured for 15 days within BAPs (n = 4–7). Results from independent experiments (n = 4–7) are expressed as mean ± SEM. *p 

    Techniques Used: Cell Culture, Positive Control, Negative Control, Activity Assay, Quantitative RT-PCR, Expressing

    Effect of O 2  strategy on the maturation of neonate pig islets (NPIs) embarked in O 2  balanced BAPs. The analyses were performed on pre-encapsulated NPIs 24 h after isolation (day 1) and on encapsulated NPIs cultured for 8 or 15 days within BAPs in 20% O 2  (positive control) and 1% O 2  conditions without the O 2  strategy (1% O 2 , negative control) or with the O 2  strategy composed of silicone-CaO 2  disk and 500 µg/mL HEMOXCell by alginate (1% O 2  + ISO) (n = 4–7 pigs). ( A ) Immunostaining of insulin ß cells (green, Alexa-Fluor 488) and glucagon α cells (red, Alexa-Fluor 555) staining of NPIs in 4 µm thick cross-sections. Views in white light are shown to the lower-left of each photo. The scale is indicated in each picture. ( B ) Percentage of mean insulin-positive area per islet (Insulin) and percentage of mean glucagon-positive area per islet (Glucagon). The percentages of insulin and glucagon were quantified within 150 islets in day 1 (n = 4 pigs) and an average of 30 islets per condition after day 8 of culture (n = 3 pigs). ( C ) Intracellular insulin by ATP content ratio. ( D ) Relative quantitative RT-PCR expression analysis of insulin ( INS ), glucagon ( GCG ), pancreatic progenitor transcription factor ( PDX1 ) and NKX6.1 ( NKX6.1 ). *p 
    Figure Legend Snippet: Effect of O 2 strategy on the maturation of neonate pig islets (NPIs) embarked in O 2 balanced BAPs. The analyses were performed on pre-encapsulated NPIs 24 h after isolation (day 1) and on encapsulated NPIs cultured for 8 or 15 days within BAPs in 20% O 2 (positive control) and 1% O 2 conditions without the O 2 strategy (1% O 2 , negative control) or with the O 2 strategy composed of silicone-CaO 2 disk and 500 µg/mL HEMOXCell by alginate (1% O 2  + ISO) (n = 4–7 pigs). ( A ) Immunostaining of insulin ß cells (green, Alexa-Fluor 488) and glucagon α cells (red, Alexa-Fluor 555) staining of NPIs in 4 µm thick cross-sections. Views in white light are shown to the lower-left of each photo. The scale is indicated in each picture. ( B ) Percentage of mean insulin-positive area per islet (Insulin) and percentage of mean glucagon-positive area per islet (Glucagon). The percentages of insulin and glucagon were quantified within 150 islets in day 1 (n = 4 pigs) and an average of 30 islets per condition after day 8 of culture (n = 3 pigs). ( C ) Intracellular insulin by ATP content ratio. ( D ) Relative quantitative RT-PCR expression analysis of insulin ( INS ), glucagon ( GCG ), pancreatic progenitor transcription factor ( PDX1 ) and NKX6.1 ( NKX6.1 ). *p 

    Techniques Used: Isolation, Cell Culture, Positive Control, Negative Control, Immunostaining, Staining, Quantitative RT-PCR, Expressing

    6) Product Images from "Induction of AML Preleukemic Fusion Genes in HSPCs and DNA Damage Response in Preleukemic Fusion Gene Positive Samples"

    Article Title: Induction of AML Preleukemic Fusion Genes in HSPCs and DNA Damage Response in Preleukemic Fusion Gene Positive Samples

    Journal: Antioxidants

    doi: 10.3390/antiox10030481

    Incidence of acute myeloid leukemia (AML) preleukemic fusion genes (PFGs) in umbilical cord blood (UCB) of Slovak newborns. The figure shows the number of PFG negative and positive samples detected by real time quantitative PCR (RT-qPCR) and validated by standard PCR and sequencing.
    Figure Legend Snippet: Incidence of acute myeloid leukemia (AML) preleukemic fusion genes (PFGs) in umbilical cord blood (UCB) of Slovak newborns. The figure shows the number of PFG negative and positive samples detected by real time quantitative PCR (RT-qPCR) and validated by standard PCR and sequencing.

    Techniques Used: Real-time Polymerase Chain Reaction, Quantitative RT-PCR, Polymerase Chain Reaction, Sequencing

    7) Product Images from "Hypoxia-Induced FAM13A Regulates the Proliferation and Metastasis of Non-Small Cell Lung Cancer Cells"

    Article Title: Hypoxia-Induced FAM13A Regulates the Proliferation and Metastasis of Non-Small Cell Lung Cancer Cells

    Journal: International Journal of Molecular Sciences

    doi: 10.3390/ijms22094302

    Generating lung cancer cell lines with FAM13A knockdown. FAM13A shRNAs (FAM13Ash1, FAM13Ash2) and control shRNAs (CtrNT2, CtrSCR) lentiviral particles were used to generate the stable transduction of A549 and CORL-105 cell lines. Transduction efficiency was assessed with the GFP marker. Knockdown of FAM13A mRNA and FAM13A protein was confirmed by real-time quantitative PCR and western-blot analysis. ( A ) Schematic indicating the binding site for the shRNA in the FAM13A mRNA. Two shRNA transcripts were designed using UCSC Broad Institute GPP Web Portal, against a coding sequence common to the most FAM13A isoforms with the RhoGAP functional domain. ( B ) RT-qPCR analysis of FAM13A expression in A549 and CORL-105 cell lines cultured under normoxia and hypoxia conditions for 72 h. Relative expression of FAM13A mRNA was determined as the mean normalized expression of FAM13A mRNA/GUSβ mRNA. The experiments were performed in triplicate and repeated three times. The data are presented as the mean ± SD (* p
    Figure Legend Snippet: Generating lung cancer cell lines with FAM13A knockdown. FAM13A shRNAs (FAM13Ash1, FAM13Ash2) and control shRNAs (CtrNT2, CtrSCR) lentiviral particles were used to generate the stable transduction of A549 and CORL-105 cell lines. Transduction efficiency was assessed with the GFP marker. Knockdown of FAM13A mRNA and FAM13A protein was confirmed by real-time quantitative PCR and western-blot analysis. ( A ) Schematic indicating the binding site for the shRNA in the FAM13A mRNA. Two shRNA transcripts were designed using UCSC Broad Institute GPP Web Portal, against a coding sequence common to the most FAM13A isoforms with the RhoGAP functional domain. ( B ) RT-qPCR analysis of FAM13A expression in A549 and CORL-105 cell lines cultured under normoxia and hypoxia conditions for 72 h. Relative expression of FAM13A mRNA was determined as the mean normalized expression of FAM13A mRNA/GUSβ mRNA. The experiments were performed in triplicate and repeated three times. The data are presented as the mean ± SD (* p

    Techniques Used: Transduction, Marker, Real-time Polymerase Chain Reaction, Western Blot, Binding Assay, shRNA, Sequencing, Functional Assay, Quantitative RT-PCR, Expressing, Cell Culture

    8) Product Images from "RNA Secondary Structure Motifs of the Influenza A Virus as Targets for siRNA-Mediated RNA Interference"

    Article Title: RNA Secondary Structure Motifs of the Influenza A Virus as Targets for siRNA-Mediated RNA Interference

    Journal: Molecular Therapy. Nucleic Acids

    doi: 10.1016/j.omtn.2019.12.018

    Inhibitory Potential of siRNAs 613′, 682, 1312′, and 1342′ against A/PR/8/34 (H1N1) in MDCK Cells Quantitative analysis of viral RNA copy number by real-time PCR was carried out for siRNA-treated samples (8 nM) and compared to negative control K1 (established as 100%). LF is a Lipofectamine-treated control. The error bars represent the standard deviations from three independent experiments. The unpaired two-tailed Student’s t test was performed for statistical comparisons (*p 
    Figure Legend Snippet: Inhibitory Potential of siRNAs 613′, 682, 1312′, and 1342′ against A/PR/8/34 (H1N1) in MDCK Cells Quantitative analysis of viral RNA copy number by real-time PCR was carried out for siRNA-treated samples (8 nM) and compared to negative control K1 (established as 100%). LF is a Lipofectamine-treated control. The error bars represent the standard deviations from three independent experiments. The unpaired two-tailed Student’s t test was performed for statistical comparisons (*p 

    Techniques Used: Real-time Polymerase Chain Reaction, Negative Control, Two Tailed Test

    Inhibitory Potential of siRNAs against A/California/04/2009 (H1N1) in MDCK Cells (A) Quantitative analysis of viral RNA by real-time PCR. RNA copy number of siRNA-treated samples at 8 nM concentration was compared to the RNA copy number of negative control K1 (established as 100%). (B) Graph of viral RNA copy numbers by real-time PCR for the most effective siRNAs tested at three concentrations. Cells treated with Lipofectamine 2000 without any siRNA are marked as LF; INF stands for untreated and infected cells. The error bars represent the standard deviations from three independent experiments. The unpaired two-tailed Student’s t test was performed for statistical comparisons (*p 
    Figure Legend Snippet: Inhibitory Potential of siRNAs against A/California/04/2009 (H1N1) in MDCK Cells (A) Quantitative analysis of viral RNA by real-time PCR. RNA copy number of siRNA-treated samples at 8 nM concentration was compared to the RNA copy number of negative control K1 (established as 100%). (B) Graph of viral RNA copy numbers by real-time PCR for the most effective siRNAs tested at three concentrations. Cells treated with Lipofectamine 2000 without any siRNA are marked as LF; INF stands for untreated and infected cells. The error bars represent the standard deviations from three independent experiments. The unpaired two-tailed Student’s t test was performed for statistical comparisons (*p 

    Techniques Used: Real-time Polymerase Chain Reaction, Concentration Assay, Negative Control, Infection, Two Tailed Test

    Antiviral Activity of ASOs against A/California/04/2009 (H1N1) in MDCK Cells Quantitative real-time PCR analysis of RNA copy number in ASO-treated samples at two concentrations was compared to the viral RNA copy number of Lipofectamine-treated cells established as 100%. The error bars represent the standard deviations from three independent experiments.
    Figure Legend Snippet: Antiviral Activity of ASOs against A/California/04/2009 (H1N1) in MDCK Cells Quantitative real-time PCR analysis of RNA copy number in ASO-treated samples at two concentrations was compared to the viral RNA copy number of Lipofectamine-treated cells established as 100%. The error bars represent the standard deviations from three independent experiments.

    Techniques Used: Activity Assay, Real-time Polymerase Chain Reaction, Allele-specific Oligonucleotide

    Inhibitory Potential of Modified siRNAs at 8 nM Concentration against A/California/04/2009 (H1N1) in MDCK Cells (A and B) Quantitative analysis of viral RNA copy number performed by real-time PCR for (A) siRNAs containing 2′-fluororibonucleotides and (B) siRNAs modified with thiophosphates, 2′- O -methyl-siRNAs, and siDNA. Cells treated with Lipofectamine 2000 without any siRNA are marked as LF. The error bars represent the standard deviations from three independent experiments. The unpaired two-tailed Student’s t test was performed for statistical comparisons (*p 
    Figure Legend Snippet: Inhibitory Potential of Modified siRNAs at 8 nM Concentration against A/California/04/2009 (H1N1) in MDCK Cells (A and B) Quantitative analysis of viral RNA copy number performed by real-time PCR for (A) siRNAs containing 2′-fluororibonucleotides and (B) siRNAs modified with thiophosphates, 2′- O -methyl-siRNAs, and siDNA. Cells treated with Lipofectamine 2000 without any siRNA are marked as LF. The error bars represent the standard deviations from three independent experiments. The unpaired two-tailed Student’s t test was performed for statistical comparisons (*p 

    Techniques Used: Modification, Concentration Assay, Real-time Polymerase Chain Reaction, Two Tailed Test

    9) Product Images from "Protein profile in Aspergillus nidulans recombinant strains overproducing heterologous enzymes"

    Article Title: Protein profile in Aspergillus nidulans recombinant strains overproducing heterologous enzymes

    Journal: Microbial Biotechnology

    doi: 10.1111/1751-7915.13027

    Secretion of total proteins by Aspergillus nidulans strains. The A. nidulans strains A773, Anid_ pEXPYR (carrying an empty pEXPYR vector), Anid_AbfA and Anid_Cbhl expressing α‐L‐arabinofuranosidase and cellobiohydrolase, respectively, were grown on minimum media containing 2% maltose for 24 h and 72 h at 37 °C. (A) Ten micrograms of secreted proteins was resolved by Coomassie blue‐staining SDS ‐ PAGE gel. The strains A773 and Anid_ pEXPYR were used as a control in this experiment. Asterisks (*) indicated the recombinant proteins. (B) qPCR of the recombinant genes was calculated by the relative standard curve method. The expression of genes abfA and cbhl was normalized using the gene tubC (tubulin) as reference. MM : molecular marker.
    Figure Legend Snippet: Secretion of total proteins by Aspergillus nidulans strains. The A. nidulans strains A773, Anid_ pEXPYR (carrying an empty pEXPYR vector), Anid_AbfA and Anid_Cbhl expressing α‐L‐arabinofuranosidase and cellobiohydrolase, respectively, were grown on minimum media containing 2% maltose for 24 h and 72 h at 37 °C. (A) Ten micrograms of secreted proteins was resolved by Coomassie blue‐staining SDS ‐ PAGE gel. The strains A773 and Anid_ pEXPYR were used as a control in this experiment. Asterisks (*) indicated the recombinant proteins. (B) qPCR of the recombinant genes was calculated by the relative standard curve method. The expression of genes abfA and cbhl was normalized using the gene tubC (tubulin) as reference. MM : molecular marker.

    Techniques Used: Plasmid Preparation, Expressing, Staining, SDS Page, Recombinant, Real-time Polymerase Chain Reaction, Marker

    10) Product Images from "Detection of Murine Astrovirus and Myocoptes musculinus in individually ventilated caging systems: Investigations to expose suitable detection methods for routine hygienic monitoring"

    Article Title: Detection of Murine Astrovirus and Myocoptes musculinus in individually ventilated caging systems: Investigations to expose suitable detection methods for routine hygienic monitoring

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0221118

    Comparison of qPCR results for soiled-bedding sentinels (SBS) and Exhaust Air Particle (EAP) monitoring at known M . musculinus prevalence. M . musculinus = Myocoptes musculinus; Exp. rp. = Experimental repetition; SBS = Soiled bedding sentinels; EAP = Exhaust air particle; ext. qPCR = quantitative PCR performed at an external provider; pos = positive EAP qPCR result for M . musculinus ; neg = negative EAP qPCR result for M . musculinus ; numerals = number of positive Sentinels per number sentinels tested for M . musculinus qPCR or serology dash, not tested; asterisk, samples between 0–1 copy / μL.
    Figure Legend Snippet: Comparison of qPCR results for soiled-bedding sentinels (SBS) and Exhaust Air Particle (EAP) monitoring at known M . musculinus prevalence. M . musculinus = Myocoptes musculinus; Exp. rp. = Experimental repetition; SBS = Soiled bedding sentinels; EAP = Exhaust air particle; ext. qPCR = quantitative PCR performed at an external provider; pos = positive EAP qPCR result for M . musculinus ; neg = negative EAP qPCR result for M . musculinus ; numerals = number of positive Sentinels per number sentinels tested for M . musculinus qPCR or serology dash, not tested; asterisk, samples between 0–1 copy / μL.

    Techniques Used: Real-time Polymerase Chain Reaction

    Comparison of qPCR and serology results for soiled-bedding sentinel (SBS) and Exhaust Air Particle (EAP) monitoring at known prevalence of MuAstV. MuAstV = Murine Astrovirus; Exp. rp. = Experimental repetition; SBS = Soiled bedding sentinels; EAP = Exhaust air particle; ext. qPCR = quantitative PCR performed at an external provider; pos = positive EAP qPCR result for MuAstV; neg = negative EAP qPCR result for MuAstV; numerals = number of positive Sentinels per number sentinels tested for qPCR or serology dash, not tested; asterisk, samples between 0–1 copy / μL.
    Figure Legend Snippet: Comparison of qPCR and serology results for soiled-bedding sentinel (SBS) and Exhaust Air Particle (EAP) monitoring at known prevalence of MuAstV. MuAstV = Murine Astrovirus; Exp. rp. = Experimental repetition; SBS = Soiled bedding sentinels; EAP = Exhaust air particle; ext. qPCR = quantitative PCR performed at an external provider; pos = positive EAP qPCR result for MuAstV; neg = negative EAP qPCR result for MuAstV; numerals = number of positive Sentinels per number sentinels tested for qPCR or serology dash, not tested; asterisk, samples between 0–1 copy / μL.

    Techniques Used: Real-time Polymerase Chain Reaction

    11) Product Images from "DUX4 regulates oocyte to embryo transition in human"

    Article Title: DUX4 regulates oocyte to embryo transition in human

    Journal: bioRxiv

    doi: 10.1101/732289

    Induction of DUX4 in the DUX4 TetOn hESCs leads to expression of intergenic genome. (a) The DUX4-ires-EmGFP piggyBac vector used to establish the doxicycline inducible DUX4 TetOn hESCs in H1 (clones 2 and 8) and H9 (clones 3 and 4). (b) DUX4 TetOn hESC clones (as above) +/- 1 μg/ml doxicycline for 3 h and live imaged for EmGFP. (c) mRNA expression kinetics of DUX4, ZSCAN4, and TRIM48 after 1 h, 2 h, and 3 h doxicycline induction measured using qRT-PCR. The data for the DUX4 TetOn H1 clone 2 is shown. Similar expression patterns were also found for the H1 clone 8, and H9 clones 3 and 4. (d) DUX4 TetOn hESCs treated with 1 μg/ml doxicycline for 4 h, fixed, and immunostained for DUX4. Representative images for nuclear DUX4 staining are shown for the H1 clone 2. Similar staining pattern were seen for the H1 clone 8 and H9 clones 3 and 4. Scale bar 50 μm. (e) Enrichment analysis of the DUX4-induced TFEs with 816 publicly available ChIP-seq datasets. A total of 7,216 ChIP-seq data for transcription factors are shown. ChIP-seq data for DUX4 are shown in red. Dots on the left side of the dashed line are underrepresented, whereas dots on the right side are overrepresented. (f) ATAC-seq intensity of human early embryo around the gained, non-significant (NS), and lost ATAC-seq peaks after DUX4 induction which overlap with ERVL-MaLR elements.
    Figure Legend Snippet: Induction of DUX4 in the DUX4 TetOn hESCs leads to expression of intergenic genome. (a) The DUX4-ires-EmGFP piggyBac vector used to establish the doxicycline inducible DUX4 TetOn hESCs in H1 (clones 2 and 8) and H9 (clones 3 and 4). (b) DUX4 TetOn hESC clones (as above) +/- 1 μg/ml doxicycline for 3 h and live imaged for EmGFP. (c) mRNA expression kinetics of DUX4, ZSCAN4, and TRIM48 after 1 h, 2 h, and 3 h doxicycline induction measured using qRT-PCR. The data for the DUX4 TetOn H1 clone 2 is shown. Similar expression patterns were also found for the H1 clone 8, and H9 clones 3 and 4. (d) DUX4 TetOn hESCs treated with 1 μg/ml doxicycline for 4 h, fixed, and immunostained for DUX4. Representative images for nuclear DUX4 staining are shown for the H1 clone 2. Similar staining pattern were seen for the H1 clone 8 and H9 clones 3 and 4. Scale bar 50 μm. (e) Enrichment analysis of the DUX4-induced TFEs with 816 publicly available ChIP-seq datasets. A total of 7,216 ChIP-seq data for transcription factors are shown. ChIP-seq data for DUX4 are shown in red. Dots on the left side of the dashed line are underrepresented, whereas dots on the right side are overrepresented. (f) ATAC-seq intensity of human early embryo around the gained, non-significant (NS), and lost ATAC-seq peaks after DUX4 induction which overlap with ERVL-MaLR elements.

    Techniques Used: Expressing, Plasmid Preparation, Clone Assay, Quantitative RT-PCR, Staining, Chromatin Immunoprecipitation

    12) Product Images from "Improved sample treatment protocol for accurate detection of live Salmonella spp. in food samples by viability PCR"

    Article Title: Improved sample treatment protocol for accurate detection of live Salmonella spp. in food samples by viability PCR

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0189302

    Effect of PEMAX on serial dilutions of live Salmonella cells in the presence of dead cells. Serial dilutions of live Salmonella cells (5.0×10 7 to 5.0×10 3 cfu) in the presence of 5.0×10 7 dead Salmonella cells were treated with 0 μM (white bars) or 100 μM (grey bars) PEMAX followed by real-time PCR. The control samples contains either 5.0×10 7 live or dead cells (no mixed population). Means, standard deviations (n = 4) and the linear regression equation and R 2 value of PEMAX treated samples are depicted. * In 3 of 4 dead samples treated with PEMAX no PCR signal were detected, therefore only the PCR signal of 1 sample is indicated in the diagram.
    Figure Legend Snippet: Effect of PEMAX on serial dilutions of live Salmonella cells in the presence of dead cells. Serial dilutions of live Salmonella cells (5.0×10 7 to 5.0×10 3 cfu) in the presence of 5.0×10 7 dead Salmonella cells were treated with 0 μM (white bars) or 100 μM (grey bars) PEMAX followed by real-time PCR. The control samples contains either 5.0×10 7 live or dead cells (no mixed population). Means, standard deviations (n = 4) and the linear regression equation and R 2 value of PEMAX treated samples are depicted. * In 3 of 4 dead samples treated with PEMAX no PCR signal were detected, therefore only the PCR signal of 1 sample is indicated in the diagram.

    Techniques Used: Real-time Polymerase Chain Reaction, Polymerase Chain Reaction

    13) Product Images from "Organic Nitrogen-Driven Stimulation of Arbuscular Mycorrhizal Fungal Hyphae Correlates with Abundance of Ammonia Oxidizers"

    Article Title: Organic Nitrogen-Driven Stimulation of Arbuscular Mycorrhizal Fungal Hyphae Correlates with Abundance of Ammonia Oxidizers

    Journal: Frontiers in Microbiology

    doi: 10.3389/fmicb.2016.00711

    Correlation analysis showing consistency of the developmental response of mycorrhizal fungal hyphae in the different pot compartments (including the rooted and root-free compartments and the differentially enriched soil patches) as assessed microscopically (x-axis) and by qPCR (y-axis). Data were standardized with the values obtained from respective plant compartments and further log (x+1) transformed for the statistical analysis. Data and regression line for R. irregularis -inoculated pots are shown as closed circles and a dashed line; data and regression for pots with C. claroideum are shown as open circles and a dotted line ( R 2 = 0.47 for R. irregularis and R 2 = 0.39 for C. claroideum ). Regression line from the analysis pooling all data together is shown as a solid line ( R 2 = 0.37). All linear regressions are highly significant, with p
    Figure Legend Snippet: Correlation analysis showing consistency of the developmental response of mycorrhizal fungal hyphae in the different pot compartments (including the rooted and root-free compartments and the differentially enriched soil patches) as assessed microscopically (x-axis) and by qPCR (y-axis). Data were standardized with the values obtained from respective plant compartments and further log (x+1) transformed for the statistical analysis. Data and regression line for R. irregularis -inoculated pots are shown as closed circles and a dashed line; data and regression for pots with C. claroideum are shown as open circles and a dotted line ( R 2 = 0.47 for R. irregularis and R 2 = 0.39 for C. claroideum ). Regression line from the analysis pooling all data together is shown as a solid line ( R 2 = 0.37). All linear regressions are highly significant, with p

    Techniques Used: Real-time Polymerase Chain Reaction, Transformation Assay

    Development of arbuscular mycorrhizal (AM) fungal hyphae in different system compartments as assessed microscopically ( A ) and using quantitative real-time PCR (qPCR; B ). Shown are relative values with respect to plant compartment which were further log (x+1) transformed for statistical comparisons. Means +1 standard error are shown. Combined microscopic data across both harvests (6 and 9 weeks) are shown in (A) , whereas the molecular data (B) were collected at the second harvest. Black bars represent pots inoculated with Rhizophagus irregularis and white bars pots with Claroideoglomus claroideum . Treatments marked with an asterisk indicate a consistent positive effect of the soil amendment on mycorrhizal hyphal development ( p
    Figure Legend Snippet: Development of arbuscular mycorrhizal (AM) fungal hyphae in different system compartments as assessed microscopically ( A ) and using quantitative real-time PCR (qPCR; B ). Shown are relative values with respect to plant compartment which were further log (x+1) transformed for statistical comparisons. Means +1 standard error are shown. Combined microscopic data across both harvests (6 and 9 weeks) are shown in (A) , whereas the molecular data (B) were collected at the second harvest. Black bars represent pots inoculated with Rhizophagus irregularis and white bars pots with Claroideoglomus claroideum . Treatments marked with an asterisk indicate a consistent positive effect of the soil amendment on mycorrhizal hyphal development ( p

    Techniques Used: Real-time Polymerase Chain Reaction, Transformation Assay

    Abundance of selected prokaryotic taxa ( Nitrosospira sp. – A , and a prokaryote similar to Acanthamoeba endosymbiont – C ) in the soil of different system compartments as detected by novel qPCR markers (see Supplementary Table S1 for details) and correlation between abundance of the two prokaryotic taxa and the developmental response of AM fungal hyphae to the different soil amendments in the root-free patches ( B,D , respectively) assessed by qPCR with AM taxa-specific markers. Black bars, closed circles, and dashed regression lines refer to pots inoculated with R. irregularis , whereas white bars, open symbols, and dotted regression lines refer to pots inoculated with C. claroideum . Bars represent means ( n = 4) with associated standard errors. Solid regression lines show the correlations of all data pooled across the two inoculation treatments. All plotted correlations were significant at the level of p
    Figure Legend Snippet: Abundance of selected prokaryotic taxa ( Nitrosospira sp. – A , and a prokaryote similar to Acanthamoeba endosymbiont – C ) in the soil of different system compartments as detected by novel qPCR markers (see Supplementary Table S1 for details) and correlation between abundance of the two prokaryotic taxa and the developmental response of AM fungal hyphae to the different soil amendments in the root-free patches ( B,D , respectively) assessed by qPCR with AM taxa-specific markers. Black bars, closed circles, and dashed regression lines refer to pots inoculated with R. irregularis , whereas white bars, open symbols, and dotted regression lines refer to pots inoculated with C. claroideum . Bars represent means ( n = 4) with associated standard errors. Solid regression lines show the correlations of all data pooled across the two inoculation treatments. All plotted correlations were significant at the level of p

    Techniques Used: Real-time Polymerase Chain Reaction

    14) Product Images from "High prevalence of intestinal parasite infestations among stunted and control children aged 2 to 5 years old in two neighborhoods of Antananarivo, Madagascar"

    Article Title: High prevalence of intestinal parasite infestations among stunted and control children aged 2 to 5 years old in two neighborhoods of Antananarivo, Madagascar

    Journal: PLoS Neglected Tropical Diseases

    doi: 10.1371/journal.pntd.0009333

    Microscopy versus real-time PCR positive results for  G .  intestinalis ,  A .  lumbricoides  and  E .  histolytica . Boxplot for A),  G .  intestinalis , for B)  E .  histolytica  and for C)  A .  lumbricoides . X axis of all boxplot indicated microscopy result of which sign “-”means negative result and sign “+” positive result and Y axis indicate Ct value of the real time PCR.
    Figure Legend Snippet: Microscopy versus real-time PCR positive results for G . intestinalis , A . lumbricoides and E . histolytica . Boxplot for A), G . intestinalis , for B) E . histolytica and for C) A . lumbricoides . X axis of all boxplot indicated microscopy result of which sign “-”means negative result and sign “+” positive result and Y axis indicate Ct value of the real time PCR.

    Techniques Used: Microscopy, Real-time Polymerase Chain Reaction

    Comparison of the prevalence of three parasites determined by real-time PCR and microscopy techniques. Blue bar indicates species determined by real-time PCR and red bar, species determined by microscopic techniques.
    Figure Legend Snippet: Comparison of the prevalence of three parasites determined by real-time PCR and microscopy techniques. Blue bar indicates species determined by real-time PCR and red bar, species determined by microscopic techniques.

    Techniques Used: Real-time Polymerase Chain Reaction, Microscopy

    Relationship between real-time PCR and microscopy (Kato-Katz thick smear technique: KK) results for  Ascaris lumbricoides . The figure displays the  A .  lumbricoides  Ct values obtained by real-time PCR as a function of the mean of number of eggs/gram (EPG) measured by KK from the same stool.
    Figure Legend Snippet: Relationship between real-time PCR and microscopy (Kato-Katz thick smear technique: KK) results for Ascaris lumbricoides . The figure displays the A . lumbricoides Ct values obtained by real-time PCR as a function of the mean of number of eggs/gram (EPG) measured by KK from the same stool.

    Techniques Used: Real-time Polymerase Chain Reaction, Microscopy

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    Solis BioDyne hot firepol probe qpcr mix plus
    Seasonal changes in the honey bee midgut/pyloric microbiota. A) Mean microbiota composition throughout a foraging season between May and October for the four dominating components found in the honey bee midgut/pylorus by <t>mixed</t> sequencing. The MCR-ALS score, determined by mixed sequencing, represents an approximately relative bacterial composition in the honey bee midgut/pylorus without assuming closure of the system. B) α-diversity between May and October calculated from the raw spectra of mixed sequencing. C) Mean bacterial load for each month between May and October. The calculated relative ratio between 16S rRNA genes and vitellogenin genes (bacteria/bee), in the midgut/pylorus determined by quantitative PCR, is shown. Significant differences were observed between May and October (one-way ANOVA p =
    Hot Firepol Probe Qpcr Mix Plus, supplied by Solis BioDyne, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/hot firepol probe qpcr mix plus/product/Solis BioDyne
    Average 97 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    hot firepol probe qpcr mix plus - by Bioz Stars, 2022-07
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    Solis BioDyne hot firepol probe universal qpcr mix plus rox
     Melt curves of  Candida auris  and  C. haemulonii  real-time polymerase chain reaction products
    Hot Firepol Probe Universal Qpcr Mix Plus Rox, supplied by Solis BioDyne, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/hot firepol probe universal qpcr mix plus rox/product/Solis BioDyne
    Average 95 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    hot firepol probe universal qpcr mix plus rox - by Bioz Stars, 2022-07
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    Seasonal changes in the honey bee midgut/pyloric microbiota. A) Mean microbiota composition throughout a foraging season between May and October for the four dominating components found in the honey bee midgut/pylorus by mixed sequencing. The MCR-ALS score, determined by mixed sequencing, represents an approximately relative bacterial composition in the honey bee midgut/pylorus without assuming closure of the system. B) α-diversity between May and October calculated from the raw spectra of mixed sequencing. C) Mean bacterial load for each month between May and October. The calculated relative ratio between 16S rRNA genes and vitellogenin genes (bacteria/bee), in the midgut/pylorus determined by quantitative PCR, is shown. Significant differences were observed between May and October (one-way ANOVA p =

    Journal: Microbes and Environments

    Article Title: Shifts in the Midgut/Pyloric Microbiota Composition within a Honey Bee Apiary throughout a Season

    doi: 10.1264/jsme2.ME15019

    Figure Lengend Snippet: Seasonal changes in the honey bee midgut/pyloric microbiota. A) Mean microbiota composition throughout a foraging season between May and October for the four dominating components found in the honey bee midgut/pylorus by mixed sequencing. The MCR-ALS score, determined by mixed sequencing, represents an approximately relative bacterial composition in the honey bee midgut/pylorus without assuming closure of the system. B) α-diversity between May and October calculated from the raw spectra of mixed sequencing. C) Mean bacterial load for each month between May and October. The calculated relative ratio between 16S rRNA genes and vitellogenin genes (bacteria/bee), in the midgut/pylorus determined by quantitative PCR, is shown. Significant differences were observed between May and October (one-way ANOVA p =

    Article Snippet: The same primer pair as that used for mixed sequencing was applied, and we used 5× HOT FIREPol Probe qPCR Mix Plus (Solis BioDyne) in a 1× concentration, with 1 μL of diluted gDNA (1:2) at a final volume of 20 μL, with the following cycling: Activation 95°C for 15 min and 40 cycles of 95°C for 30 s, 60°C for 60 s.

    Techniques: Sequencing, Real-time Polymerase Chain Reaction

    Effect of TPP + derivatives on the parasite load in T . cruzi -infected cells. RAW 264.7 cells were infected with T . cruzi trypomastigotes (Y strain); the cells were treated for 48 hours with the different derivatives, and the parasite load was assessed by qPCR. The results are expressed as the relative quantification of the parasite DNA and mammalian DNA, with a ratio of 1 assigned to the control. Treated cells were compared with the control using the ∆∆ C T method. *: p

    Journal: PLoS ONE

    Article Title: Novel Gallate Triphenylphosphonium Derivatives with Potent Antichagasic Activity

    doi: 10.1371/journal.pone.0136852

    Figure Lengend Snippet: Effect of TPP + derivatives on the parasite load in T . cruzi -infected cells. RAW 264.7 cells were infected with T . cruzi trypomastigotes (Y strain); the cells were treated for 48 hours with the different derivatives, and the parasite load was assessed by qPCR. The results are expressed as the relative quantification of the parasite DNA and mammalian DNA, with a ratio of 1 assigned to the control. Treated cells were compared with the control using the ∆∆ C T method. *: p

    Article Snippet: The reaction mixture had a final volume of 20 μL and contained 10 ng of genomic DNA, 4 μL of HOT FIREPol Probe qPCR Mix Plus (Solis BioDyne, Tallinn, Estonia), 200 nM of each primer, and 100 nM of the TcSt-1 probe or 200 nM of the MM-1 probe.

    Techniques: Infection, Real-time Polymerase Chain Reaction

    Quantitative real-time PCR data of the pro-inflammatory cytokine mRNA levels in osteoporosis (OP) and osteoarthritis (OA) . Ligand to receptor ratios for each of the studied cytokine ligand receptor pairs were calculated from mRNA values normalized to geometric mean of  GAPDH  and  RPLP0  mRNA. Values are medians. Comparisons were assessed by the Mann-Whitney  U  test, * p  values

    Journal: Journal of Biomedical Science

    Article Title: The relationship between osteoclastogenic and anti-osteoclastogenic pro-inflammatory cytokines differs in human osteoporotic and osteoarthritic bone tissues

    doi: 10.1186/1423-0127-19-28

    Figure Lengend Snippet: Quantitative real-time PCR data of the pro-inflammatory cytokine mRNA levels in osteoporosis (OP) and osteoarthritis (OA) . Ligand to receptor ratios for each of the studied cytokine ligand receptor pairs were calculated from mRNA values normalized to geometric mean of GAPDH and RPLP0 mRNA. Values are medians. Comparisons were assessed by the Mann-Whitney U test, * p values

    Article Snippet: Real time quantification was carried out on LightCycler 480 (Roche Diagnostics Ltd, Rotkreuz, Switzerland) using 5× HOT FIREPol EvaGreen qPCR Mix Plus for oligonucleotides and 5× HOT FIREPol Probe qPCR Mix Plus (Solis BioDyne, Tartu, Estonia) for TaqMan assays.

    Techniques: Real-time Polymerase Chain Reaction, MANN-WHITNEY

     Melt curves of  Candida auris  and  C. haemulonii  real-time polymerase chain reaction products

    Journal: Current Medical Mycology

    Article Title: Development a hydrolysis probe-based quantitative PCR assay for the specific detection and quantification of Candida auris

    doi: 10.18502/cmm.6.3.4665

    Figure Lengend Snippet: Melt curves of Candida auris and C. haemulonii real-time polymerase chain reaction products

    Article Snippet: Each reaction consisted of 1× HOT FIREPol Probe Universal qPCR Mix Plus ROX (Solis BioDyne, Tartu, Estonia), 0.3 μmol/L of each primer, 0.15 μmol/L of probe, and 2 μL of DNA template.

    Techniques: Real-time Polymerase Chain Reaction

    Left) linearity of hydrolysis probe-based quantitative polymerase chain reaction (qPCR) assays (10-fold serial dilutions of C. auris DNA were amplified.) and right) qPCR standard cure plotted with 10-fold serial dilutions of C. auris DNA

    Journal: Current Medical Mycology

    Article Title: Development a hydrolysis probe-based quantitative PCR assay for the specific detection and quantification of Candida auris

    doi: 10.18502/cmm.6.3.4665

    Figure Lengend Snippet: Left) linearity of hydrolysis probe-based quantitative polymerase chain reaction (qPCR) assays (10-fold serial dilutions of C. auris DNA were amplified.) and right) qPCR standard cure plotted with 10-fold serial dilutions of C. auris DNA

    Article Snippet: Each reaction consisted of 1× HOT FIREPol Probe Universal qPCR Mix Plus ROX (Solis BioDyne, Tartu, Estonia), 0.3 μmol/L of each primer, 0.15 μmol/L of probe, and 2 μL of DNA template.

    Techniques: Real-time Polymerase Chain Reaction, Amplification