hot firepol probe qpcr mix plus rox  (Solis BioDyne)


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    Solis BioDyne hot firepol probe qpcr mix plus rox
    Hypoxic signature of neonate pig islets (NPIs) in O 2  balanced BAPs. Alginate encapsulated NPIs (3000 IEQ/150 µL) were cultured for 3, 8, and 15 days under 20% O 2  (20% O 2 , positive control, white bar) or 1% O 2  condition without O 2  strategy (1% O 2,  negative control, black bar) or with the innovative strategy of oxygenation (ISO) composed of silicone-CaO 2  disk and 500 µg/mL HEMOXCell by alginate (1% O 2  + ISO, grey bars). Fold change in VEGF secretion by total metabolic activity (AU/RLU) of encapsulated NPIs compared to the control cultured for 3 days under 20% O 2  without O 2  strategy. ( B ) Relative quantitative RT-PCR expression analysis of Heme oxygenase (HO-1) of decapsulated NPIs cultured for 15 days within BAPs (n = 4–7). Results from independent experiments (n = 4–7) are expressed  as mean ± SEM. *p 
    Hot Firepol Probe Qpcr Mix Plus Rox, supplied by Solis BioDyne, used in various techniques. Bioz Stars score: 95/100, based on 56 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/hot firepol probe qpcr mix plus rox/product/Solis BioDyne
    Average 95 stars, based on 56 article reviews
    Price from $9.99 to $1999.99
    hot firepol probe qpcr mix plus rox - by Bioz Stars, 2022-12
    95/100 stars

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    1) Product Images from "Optimization of an O2-balanced bioartificial pancreas for type 1 diabetes using statistical design of experiment"

    Article Title: Optimization of an O2-balanced bioartificial pancreas for type 1 diabetes using statistical design of experiment

    Journal: Scientific Reports

    doi: 10.1038/s41598-022-07887-w

    Hypoxic signature of neonate pig islets (NPIs) in O 2  balanced BAPs. Alginate encapsulated NPIs (3000 IEQ/150 µL) were cultured for 3, 8, and 15 days under 20% O 2  (20% O 2 , positive control, white bar) or 1% O 2  condition without O 2  strategy (1% O 2,  negative control, black bar) or with the innovative strategy of oxygenation (ISO) composed of silicone-CaO 2  disk and 500 µg/mL HEMOXCell by alginate (1% O 2  + ISO, grey bars). Fold change in VEGF secretion by total metabolic activity (AU/RLU) of encapsulated NPIs compared to the control cultured for 3 days under 20% O 2  without O 2  strategy. ( B ) Relative quantitative RT-PCR expression analysis of Heme oxygenase (HO-1) of decapsulated NPIs cultured for 15 days within BAPs (n = 4–7). Results from independent experiments (n = 4–7) are expressed  as mean ± SEM. *p 
    Figure Legend Snippet: Hypoxic signature of neonate pig islets (NPIs) in O 2 balanced BAPs. Alginate encapsulated NPIs (3000 IEQ/150 µL) were cultured for 3, 8, and 15 days under 20% O 2 (20% O 2 , positive control, white bar) or 1% O 2 condition without O 2 strategy (1% O 2, negative control, black bar) or with the innovative strategy of oxygenation (ISO) composed of silicone-CaO 2 disk and 500 µg/mL HEMOXCell by alginate (1% O 2  + ISO, grey bars). Fold change in VEGF secretion by total metabolic activity (AU/RLU) of encapsulated NPIs compared to the control cultured for 3 days under 20% O 2 without O 2 strategy. ( B ) Relative quantitative RT-PCR expression analysis of Heme oxygenase (HO-1) of decapsulated NPIs cultured for 15 days within BAPs (n = 4–7). Results from independent experiments (n = 4–7) are expressed as mean ± SEM. *p 

    Techniques Used: Cell Culture, Positive Control, Negative Control, Activity Assay, Quantitative RT-PCR, Expressing

    Effect of O 2  strategy on the maturation of neonate pig islets (NPIs) embarked in O 2  balanced BAPs. The analyses were performed on pre-encapsulated NPIs 24 h after isolation (day 1) and on encapsulated NPIs cultured for 8 or 15 days within BAPs in 20% O 2  (positive control) and 1% O 2  conditions without the O 2  strategy (1% O 2 , negative control) or with the O 2  strategy composed of silicone-CaO 2  disk and 500 µg/mL HEMOXCell by alginate (1% O 2  + ISO) (n = 4–7 pigs). ( A ) Immunostaining of insulin ß cells (green, Alexa-Fluor 488) and glucagon α cells (red, Alexa-Fluor 555) staining of NPIs in 4 µm thick cross-sections. Views in white light are shown to the lower-left of each photo. The scale is indicated in each picture. ( B ) Percentage of mean insulin-positive area per islet (Insulin) and percentage of mean glucagon-positive area per islet (Glucagon). The percentages of insulin and glucagon were quantified within 150 islets in day 1 (n = 4 pigs) and an average of 30 islets per condition after day 8 of culture (n = 3 pigs). ( C ) Intracellular insulin by ATP content ratio. ( D ) Relative quantitative RT-PCR expression analysis of insulin ( INS ), glucagon ( GCG ), pancreatic progenitor transcription factor ( PDX1 ) and NKX6.1 ( NKX6.1 ). *p 
    Figure Legend Snippet: Effect of O 2 strategy on the maturation of neonate pig islets (NPIs) embarked in O 2 balanced BAPs. The analyses were performed on pre-encapsulated NPIs 24 h after isolation (day 1) and on encapsulated NPIs cultured for 8 or 15 days within BAPs in 20% O 2 (positive control) and 1% O 2 conditions without the O 2 strategy (1% O 2 , negative control) or with the O 2 strategy composed of silicone-CaO 2 disk and 500 µg/mL HEMOXCell by alginate (1% O 2  + ISO) (n = 4–7 pigs). ( A ) Immunostaining of insulin ß cells (green, Alexa-Fluor 488) and glucagon α cells (red, Alexa-Fluor 555) staining of NPIs in 4 µm thick cross-sections. Views in white light are shown to the lower-left of each photo. The scale is indicated in each picture. ( B ) Percentage of mean insulin-positive area per islet (Insulin) and percentage of mean glucagon-positive area per islet (Glucagon). The percentages of insulin and glucagon were quantified within 150 islets in day 1 (n = 4 pigs) and an average of 30 islets per condition after day 8 of culture (n = 3 pigs). ( C ) Intracellular insulin by ATP content ratio. ( D ) Relative quantitative RT-PCR expression analysis of insulin ( INS ), glucagon ( GCG ), pancreatic progenitor transcription factor ( PDX1 ) and NKX6.1 ( NKX6.1 ). *p 

    Techniques Used: Isolation, Cell Culture, Positive Control, Negative Control, Immunostaining, Staining, Quantitative RT-PCR, Expressing

    2) Product Images from "Hypoxia-Induced FAM13A Regulates the Proliferation and Metastasis of Non-Small Cell Lung Cancer Cells"

    Article Title: Hypoxia-Induced FAM13A Regulates the Proliferation and Metastasis of Non-Small Cell Lung Cancer Cells

    Journal: International Journal of Molecular Sciences

    doi: 10.3390/ijms22094302

    Generating lung cancer cell lines with FAM13A knockdown. FAM13A shRNAs (FAM13Ash1, FAM13Ash2) and control shRNAs (CtrNT2, CtrSCR) lentiviral particles were used to generate the stable transduction of A549 and CORL-105 cell lines. Transduction efficiency was assessed with the GFP marker. Knockdown of FAM13A mRNA and FAM13A protein was confirmed by real-time quantitative PCR and western-blot analysis. ( A ) Schematic indicating the binding site for the shRNA in the FAM13A mRNA. Two shRNA transcripts were designed using UCSC Broad Institute GPP Web Portal, against a coding sequence common to the most FAM13A isoforms with the RhoGAP functional domain. ( B ) RT-qPCR analysis of FAM13A expression in A549 and CORL-105 cell lines cultured under normoxia and hypoxia conditions for 72 h. Relative expression of FAM13A mRNA was determined as the mean normalized expression of FAM13A mRNA/GUSβ mRNA. The experiments were performed in triplicate and repeated three times. The data are presented as the mean ± SD (* p
    Figure Legend Snippet: Generating lung cancer cell lines with FAM13A knockdown. FAM13A shRNAs (FAM13Ash1, FAM13Ash2) and control shRNAs (CtrNT2, CtrSCR) lentiviral particles were used to generate the stable transduction of A549 and CORL-105 cell lines. Transduction efficiency was assessed with the GFP marker. Knockdown of FAM13A mRNA and FAM13A protein was confirmed by real-time quantitative PCR and western-blot analysis. ( A ) Schematic indicating the binding site for the shRNA in the FAM13A mRNA. Two shRNA transcripts were designed using UCSC Broad Institute GPP Web Portal, against a coding sequence common to the most FAM13A isoforms with the RhoGAP functional domain. ( B ) RT-qPCR analysis of FAM13A expression in A549 and CORL-105 cell lines cultured under normoxia and hypoxia conditions for 72 h. Relative expression of FAM13A mRNA was determined as the mean normalized expression of FAM13A mRNA/GUSβ mRNA. The experiments were performed in triplicate and repeated three times. The data are presented as the mean ± SD (* p

    Techniques Used: Transduction, Marker, Real-time Polymerase Chain Reaction, Western Blot, Binding Assay, shRNA, Sequencing, Functional Assay, Quantitative RT-PCR, Expressing, Cell Culture

    3) Product Images from "High prevalence of intestinal parasite infestations among stunted and control children aged 2 to 5 years old in two neighborhoods of Antananarivo, Madagascar"

    Article Title: High prevalence of intestinal parasite infestations among stunted and control children aged 2 to 5 years old in two neighborhoods of Antananarivo, Madagascar

    Journal: PLoS Neglected Tropical Diseases

    doi: 10.1371/journal.pntd.0009333

    Microscopy versus real-time PCR positive results for  G .  intestinalis ,  A .  lumbricoides  and  E .  histolytica . Boxplot for A),  G .  intestinalis , for B)  E .  histolytica  and for C)  A .  lumbricoides . X axis of all boxplot indicated microscopy result of which sign “-”means negative result and sign “+” positive result and Y axis indicate Ct value of the real time PCR.
    Figure Legend Snippet: Microscopy versus real-time PCR positive results for G . intestinalis , A . lumbricoides and E . histolytica . Boxplot for A), G . intestinalis , for B) E . histolytica and for C) A . lumbricoides . X axis of all boxplot indicated microscopy result of which sign “-”means negative result and sign “+” positive result and Y axis indicate Ct value of the real time PCR.

    Techniques Used: Microscopy, Real-time Polymerase Chain Reaction

    Comparison of the prevalence of three parasites determined by real-time PCR and microscopy techniques. Blue bar indicates species determined by real-time PCR and red bar, species determined by microscopic techniques.
    Figure Legend Snippet: Comparison of the prevalence of three parasites determined by real-time PCR and microscopy techniques. Blue bar indicates species determined by real-time PCR and red bar, species determined by microscopic techniques.

    Techniques Used: Real-time Polymerase Chain Reaction, Microscopy

    Relationship between real-time PCR and microscopy (Kato-Katz thick smear technique: KK) results for  Ascaris lumbricoides . The figure displays the  A .  lumbricoides  Ct values obtained by real-time PCR as a function of the mean of number of eggs/gram (EPG) measured by KK from the same stool.
    Figure Legend Snippet: Relationship between real-time PCR and microscopy (Kato-Katz thick smear technique: KK) results for Ascaris lumbricoides . The figure displays the A . lumbricoides Ct values obtained by real-time PCR as a function of the mean of number of eggs/gram (EPG) measured by KK from the same stool.

    Techniques Used: Real-time Polymerase Chain Reaction, Microscopy

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    Solis BioDyne hot firepol probe qpcr mix plus rox
    Hot Firepol Probe Qpcr Mix Plus Rox, supplied by Solis BioDyne, used in various techniques. Bioz Stars score: 95/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/hot firepol probe qpcr mix plus rox/product/Solis BioDyne
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    Solis BioDyne hot firepol evagreen qpcr supermix
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