hot firepol probe universal qpcr mix plus rox  (Solis BioDyne)

Journal: Current Medical Mycology

Article Title: Development a hydrolysis probe-based quantitative PCR assay for the specific detection and quantification of Candida auris

doi: 10.18502/cmm.6.3.4665

Figure Lengend Snippet: Melt curves of Candida auris and C. haemulonii real-time polymerase chain reaction products

Article Snippet: Each reaction consisted of 1× HOT FIREPol Probe Universal qPCR Mix Plus ROX (Solis BioDyne, Tartu, Estonia), 0.3 μmol/L of each primer, 0.15 μmol/L of probe, and 2 μL of DNA template.

Techniques: Real-time Polymerase Chain Reaction

Journal: Current Medical Mycology

Article Title: Development a hydrolysis probe-based quantitative PCR assay for the specific detection and quantification of Candida auris

doi: 10.18502/cmm.6.3.4665

Figure Lengend Snippet: Left) linearity of hydrolysis probe-based quantitative polymerase chain reaction (qPCR) assays (10-fold serial dilutions of C. auris DNA were amplified.) and right) qPCR standard cure plotted with 10-fold serial dilutions of C. auris DNA

Article Snippet: Each reaction consisted of 1× HOT FIREPol Probe Universal qPCR Mix Plus ROX (Solis BioDyne, Tartu, Estonia), 0.3 μmol/L of each primer, 0.15 μmol/L of probe, and 2 μL of DNA template.

Techniques: Real-time Polymerase Chain Reaction, Amplification

Journal: Food Chemistry: Molecular Sciences

Article Title: Novel LNA probe-based assay for the A1 and A2 identification of β-casein gene in milk samples

doi: 10.1016/j.fochms.2021.100055

Figure Lengend Snippet: Quantitative cycle (Cq) means obtained from yellow and green channels by LNA probe and rhAMP qPCR assays using 38 DNA samples. Means followed by the different letters differ significantly (p

Article Snippet: Briefly, each reaction was carried using 5 pmol of primers (0.5 μL of 10 μM each primer solution), 2.5 pmol of each probe (0.5 μL of 2.5 μM probe solution) and 5 × HOT FIREPol Probe Universal qPCR Mix (2 μL of 5 × Mastermix solution) (Solis BioDyne, Tartu, Estonia), 4 μL of ultrapure water and 2 μL of DNA in Rotor-Gene Q thermocycler (Qiagen, Venlo, Netherlands).

Techniques: Real-time Polymerase Chain Reaction