taq dna polymerase  (Solis BioDyne)


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    Structured Review

    Solis BioDyne taq dna polymerase
    The secondary structure of the vrn - A1 promoter may prevent successful amplification and sequencing. ( a ) Schematic representation of vrn - A1 promoter variants with 137 bp, 181 bp and 194 bp deletions found in winter cultivars and the position of the G-quadruplex (G4). ( b ) Sequence motif of G4 found in Triple Dirk C (TDC, MH347747). ( c ) <t>DNA</t> fold prediction of G4 found in TDC.
    Taq Dna Polymerase, supplied by Solis BioDyne, used in various techniques. Bioz Stars score: 96/100, based on 9 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/taq dna polymerase/product/Solis BioDyne
    Average 96 stars, based on 9 article reviews
    Price from $9.99 to $1999.99
    taq dna polymerase - by Bioz Stars, 2022-12
    96/100 stars

    Images

    1) Product Images from "In-Depth Sequence Analysis of Bread Wheat VRN1 Genes"

    Article Title: In-Depth Sequence Analysis of Bread Wheat VRN1 Genes

    Journal: International Journal of Molecular Sciences

    doi: 10.3390/ijms222212284

    The secondary structure of the vrn - A1 promoter may prevent successful amplification and sequencing. ( a ) Schematic representation of vrn - A1 promoter variants with 137 bp, 181 bp and 194 bp deletions found in winter cultivars and the position of the G-quadruplex (G4). ( b ) Sequence motif of G4 found in Triple Dirk C (TDC, MH347747). ( c ) DNA fold prediction of G4 found in TDC.
    Figure Legend Snippet: The secondary structure of the vrn - A1 promoter may prevent successful amplification and sequencing. ( a ) Schematic representation of vrn - A1 promoter variants with 137 bp, 181 bp and 194 bp deletions found in winter cultivars and the position of the G-quadruplex (G4). ( b ) Sequence motif of G4 found in Triple Dirk C (TDC, MH347747). ( c ) DNA fold prediction of G4 found in TDC.

    Techniques Used: Amplification, Sequencing

    2) Product Images from "Equine Parvovirus-Hepatitis Screening in Horses and Donkeys with Histopathologic Liver Abnormalities"

    Article Title: Equine Parvovirus-Hepatitis Screening in Horses and Donkeys with Histopathologic Liver Abnormalities

    Journal: Viruses

    doi: 10.3390/v13081599

    Detection of EqPV-H ( A ) and the cellular calibrator gene TTC17 ( B ) by dPCR in liver samples #1 and #2. Each panel represents a separate experimental chamber on a chip where the reaction mix containing DNA from liver samples (1:20 dilution for TTC17 ) was segregated into individual droplets and assessed for the presence of EqPV-H and TTC17 . • : positive samples; •: negative signals, respectively. The blue line designates the arbitrary fluorescence threshold separating the signals from background.
    Figure Legend Snippet: Detection of EqPV-H ( A ) and the cellular calibrator gene TTC17 ( B ) by dPCR in liver samples #1 and #2. Each panel represents a separate experimental chamber on a chip where the reaction mix containing DNA from liver samples (1:20 dilution for TTC17 ) was segregated into individual droplets and assessed for the presence of EqPV-H and TTC17 . • : positive samples; •: negative signals, respectively. The blue line designates the arbitrary fluorescence threshold separating the signals from background.

    Techniques Used: Digital PCR, Chromatin Immunoprecipitation, Fluorescence

    3) Product Images from "Pharmacological or TRIB3-Mediated Suppression of ATF4 Transcriptional Activity Promotes Hepatoma Cell Resistance to Proteasome Inhibitor Bortezomib"

    Article Title: Pharmacological or TRIB3-Mediated Suppression of ATF4 Transcriptional Activity Promotes Hepatoma Cell Resistance to Proteasome Inhibitor Bortezomib

    Journal: Cancers

    doi: 10.3390/cancers13102341

    TRIB3 and ATF4 binding near genes that are regulated in response to bortezomib (Btz), nelfinavir (Nelf) and ISRIB in HepG2 cells. ( A ) Gene ontology enrichment analysis for genes with a TRIB3 ChIP-Seq peak within 2 kb of the transcription start site (TSS). The GO Biological Process database was used. ( B ) RNA-Seq differential expression results overlap with genes harboring TRIB3 ChIP-Seq peaks (TSS ± 2 kb). ISRIB-downregulated genes were obtained comparing ISRIB and Btz co-treatment to Btz alone. ( C ) Btz-upregulated genes with TSS-proximal (TSS ± 2 kb) TRIB3 ChIP-Seq peaks. Genes are ranked by the TRIB3 Btz ChIP-Seq signal strength. ( D ) ChIP-Seq coverage plots for selected loci. The αFlag neg. cells track represents ChIP-Seq of unedited HepG2 cells with anti-Flag antibody. ( E ) ChIP-qPCR validation for the binding of ATF4, TRIB3 (anti-Flag M2) and non-targeting IgG at selected loci detected in ChIP-Seq. A negative control locus is also shown (-Ctrl). The means ± SD of 2–5 ChIP experiments from two biological replicates are shown. The DNA quantity is presented as a percentage of input chromatin.
    Figure Legend Snippet: TRIB3 and ATF4 binding near genes that are regulated in response to bortezomib (Btz), nelfinavir (Nelf) and ISRIB in HepG2 cells. ( A ) Gene ontology enrichment analysis for genes with a TRIB3 ChIP-Seq peak within 2 kb of the transcription start site (TSS). The GO Biological Process database was used. ( B ) RNA-Seq differential expression results overlap with genes harboring TRIB3 ChIP-Seq peaks (TSS ± 2 kb). ISRIB-downregulated genes were obtained comparing ISRIB and Btz co-treatment to Btz alone. ( C ) Btz-upregulated genes with TSS-proximal (TSS ± 2 kb) TRIB3 ChIP-Seq peaks. Genes are ranked by the TRIB3 Btz ChIP-Seq signal strength. ( D ) ChIP-Seq coverage plots for selected loci. The αFlag neg. cells track represents ChIP-Seq of unedited HepG2 cells with anti-Flag antibody. ( E ) ChIP-qPCR validation for the binding of ATF4, TRIB3 (anti-Flag M2) and non-targeting IgG at selected loci detected in ChIP-Seq. A negative control locus is also shown (-Ctrl). The means ± SD of 2–5 ChIP experiments from two biological replicates are shown. The DNA quantity is presented as a percentage of input chromatin.

    Techniques Used: Binding Assay, Chromatin Immunoprecipitation, RNA Sequencing Assay, Expressing, Real-time Polymerase Chain Reaction, Negative Control

    4) Product Images from "Pharmacological or TRIB3-Mediated Suppression of ATF4 Transcriptional Activity Promotes Hepatoma Cell Resistance to Proteasome Inhibitor Bortezomib"

    Article Title: Pharmacological or TRIB3-Mediated Suppression of ATF4 Transcriptional Activity Promotes Hepatoma Cell Resistance to Proteasome Inhibitor Bortezomib

    Journal: Cancers

    doi: 10.3390/cancers13102341

    TRIB3 and ATF4 binding near genes that are regulated in response to bortezomib (Btz), nelfinavir (Nelf) and ISRIB in HepG2 cells. ( A ) Gene ontology enrichment analysis for genes with a TRIB3 ChIP-Seq peak within 2 kb of the transcription start site (TSS). The GO Biological Process database was used. ( B ) RNA-Seq differential expression results overlap with genes harboring TRIB3 ChIP-Seq peaks (TSS ± 2 kb). ISRIB-downregulated genes were obtained comparing ISRIB and Btz co-treatment to Btz alone. ( C ) Btz-upregulated genes with TSS-proximal (TSS ± 2 kb) TRIB3 ChIP-Seq peaks. Genes are ranked by the TRIB3 Btz ChIP-Seq signal strength. ( D ) ChIP-Seq coverage plots for selected loci. The αFlag neg. cells track represents ChIP-Seq of unedited HepG2 cells with anti-Flag antibody. ( E ) ChIP-qPCR validation for the binding of ATF4, TRIB3 (anti-Flag M2) and non-targeting IgG at selected loci detected in ChIP-Seq. A negative control locus is also shown (-Ctrl). The means ± SD of 2–5 ChIP experiments from two biological replicates are shown. The DNA quantity is presented as a percentage of input chromatin.
    Figure Legend Snippet: TRIB3 and ATF4 binding near genes that are regulated in response to bortezomib (Btz), nelfinavir (Nelf) and ISRIB in HepG2 cells. ( A ) Gene ontology enrichment analysis for genes with a TRIB3 ChIP-Seq peak within 2 kb of the transcription start site (TSS). The GO Biological Process database was used. ( B ) RNA-Seq differential expression results overlap with genes harboring TRIB3 ChIP-Seq peaks (TSS ± 2 kb). ISRIB-downregulated genes were obtained comparing ISRIB and Btz co-treatment to Btz alone. ( C ) Btz-upregulated genes with TSS-proximal (TSS ± 2 kb) TRIB3 ChIP-Seq peaks. Genes are ranked by the TRIB3 Btz ChIP-Seq signal strength. ( D ) ChIP-Seq coverage plots for selected loci. The αFlag neg. cells track represents ChIP-Seq of unedited HepG2 cells with anti-Flag antibody. ( E ) ChIP-qPCR validation for the binding of ATF4, TRIB3 (anti-Flag M2) and non-targeting IgG at selected loci detected in ChIP-Seq. A negative control locus is also shown (-Ctrl). The means ± SD of 2–5 ChIP experiments from two biological replicates are shown. The DNA quantity is presented as a percentage of input chromatin.

    Techniques Used: Binding Assay, Chromatin Immunoprecipitation, RNA Sequencing Assay, Expressing, Real-time Polymerase Chain Reaction, Negative Control

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    Solis BioDyne taq dna polymerase
    Taq Dna Polymerase, supplied by Solis BioDyne, used in various techniques. Bioz Stars score: 96/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/taq dna polymerase/product/Solis BioDyne
    Average 96 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    taq dna polymerase - by Bioz Stars, 2022-12
    96/100 stars
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