mouse monoclonal anti 3 nt antibody  (Hycult Biotech)


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    Hycult Biotech mouse monoclonal anti 3 nt antibody
    Mouse Monoclonal Anti 3 Nt Antibody, supplied by Hycult Biotech, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    mouse monoclonal anti 3 nt antibody  (Hycult Biotech)


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    Hycult Biotech mouse monoclonal anti 3 nt antibody
    Mouse Monoclonal Anti 3 Nt Antibody, supplied by Hycult Biotech, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    anti nitrotyrosine mouse monoclonal antibody  (Hycult Biotech)


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    Hycult Biotech anti nitrotyrosine mouse monoclonal antibody
    150 µg of TIF was loaded into the 2D gel and transferred onto a PDVF membrane. The blot was probed with <t>anti-nitrotyrosine</t> polyclonal antibody (A), after total protein SYPRO Ruby blot staining (B). Nitrated protein signals of the 2D WB were matched and localized in a twin 2D gel and proteins were identified by peptide mass fingerprinting. Spot numbers in (A) correspond to proteins in . a, b and c are spots corresponding respectively to laminin subunit beta-2, VDAC, GFAP, that were not specifically increased in G93A TIF in the proteomic screening. (C) Dot blot analysis of nitrated HSP90 in TIF of spinal cord tissues of controls (CTR) (n = 3), white bars, and sporadic ALS patients (n = 7), grey bars. The same amount of TIF (3 µg) was loaded on the membrane and probed with the specific antibody. Histograms represent the immunoreactivity normalized to the actual amount of protein loaded, as detected after Red Ponceau staining. Values are percentages of controls and are the mean±SD. *, significantly different from controls as assessed by unpaired t test with Welsh's correction ( p <0.05). Representative dot blots for a control and an ALS patient are reported.
    Anti Nitrotyrosine Mouse Monoclonal Antibody, supplied by Hycult Biotech, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti nitrotyrosine mouse monoclonal antibody/product/Hycult Biotech
    Average 93 stars, based on 1 article reviews
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    anti nitrotyrosine mouse monoclonal antibody - by Bioz Stars, 2024-05
    93/100 stars

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    1) Product Images from "Characterization of Detergent-Insoluble Proteins in ALS Indicates a Causal Link between Nitrative Stress and Aggregation in Pathogenesis"

    Article Title: Characterization of Detergent-Insoluble Proteins in ALS Indicates a Causal Link between Nitrative Stress and Aggregation in Pathogenesis

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0008130

    150 µg of TIF was loaded into the 2D gel and transferred onto a PDVF membrane. The blot was probed with anti-nitrotyrosine polyclonal antibody (A), after total protein SYPRO Ruby blot staining (B). Nitrated protein signals of the 2D WB were matched and localized in a twin 2D gel and proteins were identified by peptide mass fingerprinting. Spot numbers in (A) correspond to proteins in . a, b and c are spots corresponding respectively to laminin subunit beta-2, VDAC, GFAP, that were not specifically increased in G93A TIF in the proteomic screening. (C) Dot blot analysis of nitrated HSP90 in TIF of spinal cord tissues of controls (CTR) (n = 3), white bars, and sporadic ALS patients (n = 7), grey bars. The same amount of TIF (3 µg) was loaded on the membrane and probed with the specific antibody. Histograms represent the immunoreactivity normalized to the actual amount of protein loaded, as detected after Red Ponceau staining. Values are percentages of controls and are the mean±SD. *, significantly different from controls as assessed by unpaired t test with Welsh's correction ( p <0.05). Representative dot blots for a control and an ALS patient are reported.
    Figure Legend Snippet: 150 µg of TIF was loaded into the 2D gel and transferred onto a PDVF membrane. The blot was probed with anti-nitrotyrosine polyclonal antibody (A), after total protein SYPRO Ruby blot staining (B). Nitrated protein signals of the 2D WB were matched and localized in a twin 2D gel and proteins were identified by peptide mass fingerprinting. Spot numbers in (A) correspond to proteins in . a, b and c are spots corresponding respectively to laminin subunit beta-2, VDAC, GFAP, that were not specifically increased in G93A TIF in the proteomic screening. (C) Dot blot analysis of nitrated HSP90 in TIF of spinal cord tissues of controls (CTR) (n = 3), white bars, and sporadic ALS patients (n = 7), grey bars. The same amount of TIF (3 µg) was loaded on the membrane and probed with the specific antibody. Histograms represent the immunoreactivity normalized to the actual amount of protein loaded, as detected after Red Ponceau staining. Values are percentages of controls and are the mean±SD. *, significantly different from controls as assessed by unpaired t test with Welsh's correction ( p <0.05). Representative dot blots for a control and an ALS patient are reported.

    Techniques Used: Two-Dimensional Gel Electrophoresis, Staining, Peptide Mass Fingerprinting, Dot Blot

    (A) Total TIF is the ratio of the amount of TIF to the total proteins extracted. Proteins were quantified in each condition by the BCA protein assay. Values are percentages of the untreated WT control and are the mean±SEM (n = 3). (B–G) The level of nitrotyrosine, nitrated HSP90 (HSP90 NT ), aconitase, HSC70, CypA and SOD1 were measured by dot blot analysis. The same amount of cellular TIF (3 µg) was loaded on the membrane and probed with the specific antibodies. Histograms represent the immunoreactivity normalized to the actual amount of protein loaded, as detected after Red Ponceau staining, multiplied by the total TIF isolated for each condition. Values are percentages of the untreated WT control and are the mean±SEM (n = 3). *, significantly different from untreated G93A controls ( p <0.05); **, significantly different from MG132-treated G93A samples ( p <0.05); ***, significantly different from MG132-treated WT samples ( p <0.05), as assessed by one-way ANOVA followed by Newman-Keuls multiple comparison test. (H) Analysis of cell death by quantification of extracellular LDH activity. Histograms represent mean±SD of four replicates. One-way ANOVA was followed by Newman-Keuls multiple comparison test. *, p <0.05.
    Figure Legend Snippet: (A) Total TIF is the ratio of the amount of TIF to the total proteins extracted. Proteins were quantified in each condition by the BCA protein assay. Values are percentages of the untreated WT control and are the mean±SEM (n = 3). (B–G) The level of nitrotyrosine, nitrated HSP90 (HSP90 NT ), aconitase, HSC70, CypA and SOD1 were measured by dot blot analysis. The same amount of cellular TIF (3 µg) was loaded on the membrane and probed with the specific antibodies. Histograms represent the immunoreactivity normalized to the actual amount of protein loaded, as detected after Red Ponceau staining, multiplied by the total TIF isolated for each condition. Values are percentages of the untreated WT control and are the mean±SEM (n = 3). *, significantly different from untreated G93A controls ( p <0.05); **, significantly different from MG132-treated G93A samples ( p <0.05); ***, significantly different from MG132-treated WT samples ( p <0.05), as assessed by one-way ANOVA followed by Newman-Keuls multiple comparison test. (H) Analysis of cell death by quantification of extracellular LDH activity. Histograms represent mean±SD of four replicates. One-way ANOVA was followed by Newman-Keuls multiple comparison test. *, p <0.05.

    Techniques Used: Bicinchoninic Acid Protein Assay, Dot Blot, Staining, Isolation, Activity Assay

    mouse monoclonal anti 3 nt antibody  (Hycult Biotech)


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    Hycult Biotech mouse monoclonal anti 3 nt antibody
    Mouse Monoclonal Anti 3 Nt Antibody, supplied by Hycult Biotech, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    nitrotyrosine, mab hm.11  (Hycult Biotech)


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    Hycult Biotech nitrotyrosine, mab hm.11
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    mouse monoclonal antibody to ntyr  (Hycult Biotech)


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    Hycult Biotech mouse monoclonal antibody to ntyr
    Mouse Monoclonal Antibody To Ntyr, supplied by Hycult Biotech, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    monoclonal antibody  (Hycult Biotech)


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    Hycult Biotech monoclonal antibody
    Monoclonal Antibody, supplied by Hycult Biotech, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    mouse monoclonal anti nitrotyrosine  (Hycult Biotech)


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    Hycult Biotech mouse monoclonal anti nitrotyrosine
    Mouse Monoclonal Anti Nitrotyrosine, supplied by Hycult Biotech, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Hycult Biotech mouse monoclonal anti 3 nt antibody
    Mouse Monoclonal Anti 3 Nt Antibody, supplied by Hycult Biotech, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Hycult Biotech anti nitrotyrosine mouse monoclonal antibody
    150 µg of TIF was loaded into the 2D gel and transferred onto a PDVF membrane. The blot was probed with <t>anti-nitrotyrosine</t> polyclonal antibody (A), after total protein SYPRO Ruby blot staining (B). Nitrated protein signals of the 2D WB were matched and localized in a twin 2D gel and proteins were identified by peptide mass fingerprinting. Spot numbers in (A) correspond to proteins in . a, b and c are spots corresponding respectively to laminin subunit beta-2, VDAC, GFAP, that were not specifically increased in G93A TIF in the proteomic screening. (C) Dot blot analysis of nitrated HSP90 in TIF of spinal cord tissues of controls (CTR) (n = 3), white bars, and sporadic ALS patients (n = 7), grey bars. The same amount of TIF (3 µg) was loaded on the membrane and probed with the specific antibody. Histograms represent the immunoreactivity normalized to the actual amount of protein loaded, as detected after Red Ponceau staining. Values are percentages of controls and are the mean±SD. *, significantly different from controls as assessed by unpaired t test with Welsh's correction ( p <0.05). Representative dot blots for a control and an ALS patient are reported.
    Anti Nitrotyrosine Mouse Monoclonal Antibody, supplied by Hycult Biotech, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti nitrotyrosine mouse monoclonal antibody/product/Hycult Biotech
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    Hycult Biotech nitrotyrosine, mab hm.11
    150 µg of TIF was loaded into the 2D gel and transferred onto a PDVF membrane. The blot was probed with <t>anti-nitrotyrosine</t> polyclonal antibody (A), after total protein SYPRO Ruby blot staining (B). Nitrated protein signals of the 2D WB were matched and localized in a twin 2D gel and proteins were identified by peptide mass fingerprinting. Spot numbers in (A) correspond to proteins in . a, b and c are spots corresponding respectively to laminin subunit beta-2, VDAC, GFAP, that were not specifically increased in G93A TIF in the proteomic screening. (C) Dot blot analysis of nitrated HSP90 in TIF of spinal cord tissues of controls (CTR) (n = 3), white bars, and sporadic ALS patients (n = 7), grey bars. The same amount of TIF (3 µg) was loaded on the membrane and probed with the specific antibody. Histograms represent the immunoreactivity normalized to the actual amount of protein loaded, as detected after Red Ponceau staining. Values are percentages of controls and are the mean±SD. *, significantly different from controls as assessed by unpaired t test with Welsh's correction ( p <0.05). Representative dot blots for a control and an ALS patient are reported.
    Nitrotyrosine, Mab Hm.11, supplied by Hycult Biotech, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Hycult Biotech mouse monoclonal antibody to ntyr
    150 µg of TIF was loaded into the 2D gel and transferred onto a PDVF membrane. The blot was probed with <t>anti-nitrotyrosine</t> polyclonal antibody (A), after total protein SYPRO Ruby blot staining (B). Nitrated protein signals of the 2D WB were matched and localized in a twin 2D gel and proteins were identified by peptide mass fingerprinting. Spot numbers in (A) correspond to proteins in . a, b and c are spots corresponding respectively to laminin subunit beta-2, VDAC, GFAP, that were not specifically increased in G93A TIF in the proteomic screening. (C) Dot blot analysis of nitrated HSP90 in TIF of spinal cord tissues of controls (CTR) (n = 3), white bars, and sporadic ALS patients (n = 7), grey bars. The same amount of TIF (3 µg) was loaded on the membrane and probed with the specific antibody. Histograms represent the immunoreactivity normalized to the actual amount of protein loaded, as detected after Red Ponceau staining. Values are percentages of controls and are the mean±SD. *, significantly different from controls as assessed by unpaired t test with Welsh's correction ( p <0.05). Representative dot blots for a control and an ALS patient are reported.
    Mouse Monoclonal Antibody To Ntyr, supplied by Hycult Biotech, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mouse monoclonal antibody to ntyr/product/Hycult Biotech
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    Hycult Biotech monoclonal antibody
    150 µg of TIF was loaded into the 2D gel and transferred onto a PDVF membrane. The blot was probed with <t>anti-nitrotyrosine</t> polyclonal antibody (A), after total protein SYPRO Ruby blot staining (B). Nitrated protein signals of the 2D WB were matched and localized in a twin 2D gel and proteins were identified by peptide mass fingerprinting. Spot numbers in (A) correspond to proteins in . a, b and c are spots corresponding respectively to laminin subunit beta-2, VDAC, GFAP, that were not specifically increased in G93A TIF in the proteomic screening. (C) Dot blot analysis of nitrated HSP90 in TIF of spinal cord tissues of controls (CTR) (n = 3), white bars, and sporadic ALS patients (n = 7), grey bars. The same amount of TIF (3 µg) was loaded on the membrane and probed with the specific antibody. Histograms represent the immunoreactivity normalized to the actual amount of protein loaded, as detected after Red Ponceau staining. Values are percentages of controls and are the mean±SD. *, significantly different from controls as assessed by unpaired t test with Welsh's correction ( p <0.05). Representative dot blots for a control and an ALS patient are reported.
    Monoclonal Antibody, supplied by Hycult Biotech, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Hycult Biotech mouse monoclonal anti nitrotyrosine
    150 µg of TIF was loaded into the 2D gel and transferred onto a PDVF membrane. The blot was probed with <t>anti-nitrotyrosine</t> polyclonal antibody (A), after total protein SYPRO Ruby blot staining (B). Nitrated protein signals of the 2D WB were matched and localized in a twin 2D gel and proteins were identified by peptide mass fingerprinting. Spot numbers in (A) correspond to proteins in . a, b and c are spots corresponding respectively to laminin subunit beta-2, VDAC, GFAP, that were not specifically increased in G93A TIF in the proteomic screening. (C) Dot blot analysis of nitrated HSP90 in TIF of spinal cord tissues of controls (CTR) (n = 3), white bars, and sporadic ALS patients (n = 7), grey bars. The same amount of TIF (3 µg) was loaded on the membrane and probed with the specific antibody. Histograms represent the immunoreactivity normalized to the actual amount of protein loaded, as detected after Red Ponceau staining. Values are percentages of controls and are the mean±SD. *, significantly different from controls as assessed by unpaired t test with Welsh's correction ( p <0.05). Representative dot blots for a control and an ALS patient are reported.
    Mouse Monoclonal Anti Nitrotyrosine, supplied by Hycult Biotech, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    150 µg of TIF was loaded into the 2D gel and transferred onto a PDVF membrane. The blot was probed with anti-nitrotyrosine polyclonal antibody (A), after total protein SYPRO Ruby blot staining (B). Nitrated protein signals of the 2D WB were matched and localized in a twin 2D gel and proteins were identified by peptide mass fingerprinting. Spot numbers in (A) correspond to proteins in . a, b and c are spots corresponding respectively to laminin subunit beta-2, VDAC, GFAP, that were not specifically increased in G93A TIF in the proteomic screening. (C) Dot blot analysis of nitrated HSP90 in TIF of spinal cord tissues of controls (CTR) (n = 3), white bars, and sporadic ALS patients (n = 7), grey bars. The same amount of TIF (3 µg) was loaded on the membrane and probed with the specific antibody. Histograms represent the immunoreactivity normalized to the actual amount of protein loaded, as detected after Red Ponceau staining. Values are percentages of controls and are the mean±SD. *, significantly different from controls as assessed by unpaired t test with Welsh's correction ( p <0.05). Representative dot blots for a control and an ALS patient are reported.

    Journal: PLoS ONE

    Article Title: Characterization of Detergent-Insoluble Proteins in ALS Indicates a Causal Link between Nitrative Stress and Aggregation in Pathogenesis

    doi: 10.1371/journal.pone.0008130

    Figure Lengend Snippet: 150 µg of TIF was loaded into the 2D gel and transferred onto a PDVF membrane. The blot was probed with anti-nitrotyrosine polyclonal antibody (A), after total protein SYPRO Ruby blot staining (B). Nitrated protein signals of the 2D WB were matched and localized in a twin 2D gel and proteins were identified by peptide mass fingerprinting. Spot numbers in (A) correspond to proteins in . a, b and c are spots corresponding respectively to laminin subunit beta-2, VDAC, GFAP, that were not specifically increased in G93A TIF in the proteomic screening. (C) Dot blot analysis of nitrated HSP90 in TIF of spinal cord tissues of controls (CTR) (n = 3), white bars, and sporadic ALS patients (n = 7), grey bars. The same amount of TIF (3 µg) was loaded on the membrane and probed with the specific antibody. Histograms represent the immunoreactivity normalized to the actual amount of protein loaded, as detected after Red Ponceau staining. Values are percentages of controls and are the mean±SD. *, significantly different from controls as assessed by unpaired t test with Welsh's correction ( p <0.05). Representative dot blots for a control and an ALS patient are reported.

    Article Snippet: One 2D gel was stained with SYPRO® Ruby protein gel stain (Invitrogen) and the other was transferred onto PVDF membrane (Millipore) and probed overnight at 4°C with anti-nitrotyrosine rabbit polyclonal antibody, provided by A.G. Estevez, or the anti-nitrotyrosine mouse monoclonal antibody (clone HM.11; HyCult Biotechnology).

    Techniques: Two-Dimensional Gel Electrophoresis, Staining, Peptide Mass Fingerprinting, Dot Blot

    (A) Total TIF is the ratio of the amount of TIF to the total proteins extracted. Proteins were quantified in each condition by the BCA protein assay. Values are percentages of the untreated WT control and are the mean±SEM (n = 3). (B–G) The level of nitrotyrosine, nitrated HSP90 (HSP90 NT ), aconitase, HSC70, CypA and SOD1 were measured by dot blot analysis. The same amount of cellular TIF (3 µg) was loaded on the membrane and probed with the specific antibodies. Histograms represent the immunoreactivity normalized to the actual amount of protein loaded, as detected after Red Ponceau staining, multiplied by the total TIF isolated for each condition. Values are percentages of the untreated WT control and are the mean±SEM (n = 3). *, significantly different from untreated G93A controls ( p <0.05); **, significantly different from MG132-treated G93A samples ( p <0.05); ***, significantly different from MG132-treated WT samples ( p <0.05), as assessed by one-way ANOVA followed by Newman-Keuls multiple comparison test. (H) Analysis of cell death by quantification of extracellular LDH activity. Histograms represent mean±SD of four replicates. One-way ANOVA was followed by Newman-Keuls multiple comparison test. *, p <0.05.

    Journal: PLoS ONE

    Article Title: Characterization of Detergent-Insoluble Proteins in ALS Indicates a Causal Link between Nitrative Stress and Aggregation in Pathogenesis

    doi: 10.1371/journal.pone.0008130

    Figure Lengend Snippet: (A) Total TIF is the ratio of the amount of TIF to the total proteins extracted. Proteins were quantified in each condition by the BCA protein assay. Values are percentages of the untreated WT control and are the mean±SEM (n = 3). (B–G) The level of nitrotyrosine, nitrated HSP90 (HSP90 NT ), aconitase, HSC70, CypA and SOD1 were measured by dot blot analysis. The same amount of cellular TIF (3 µg) was loaded on the membrane and probed with the specific antibodies. Histograms represent the immunoreactivity normalized to the actual amount of protein loaded, as detected after Red Ponceau staining, multiplied by the total TIF isolated for each condition. Values are percentages of the untreated WT control and are the mean±SEM (n = 3). *, significantly different from untreated G93A controls ( p <0.05); **, significantly different from MG132-treated G93A samples ( p <0.05); ***, significantly different from MG132-treated WT samples ( p <0.05), as assessed by one-way ANOVA followed by Newman-Keuls multiple comparison test. (H) Analysis of cell death by quantification of extracellular LDH activity. Histograms represent mean±SD of four replicates. One-way ANOVA was followed by Newman-Keuls multiple comparison test. *, p <0.05.

    Article Snippet: One 2D gel was stained with SYPRO® Ruby protein gel stain (Invitrogen) and the other was transferred onto PVDF membrane (Millipore) and probed overnight at 4°C with anti-nitrotyrosine rabbit polyclonal antibody, provided by A.G. Estevez, or the anti-nitrotyrosine mouse monoclonal antibody (clone HM.11; HyCult Biotechnology).

    Techniques: Bicinchoninic Acid Protein Assay, Dot Blot, Staining, Isolation, Activity Assay