Journal: Frontiers in Immunology
Article Title: Complement Genetic Variants and FH Desialylation in S. pneumoniae -Haemolytic Uraemic Syndrome
doi: 10.3389/fimmu.2021.641656
Figure Lengend Snippet: Desialylation of FHR-1 and FHR-3 in patient H837 by 2D-WB analysis. FHR1 and FHR3 partially purified from sp1 and sp2 from patient H837 and from a control individual were subjected to 2D-electrophoresis and Western-blot with polyclonal antibodies. The characteristic ladder of FHR-3 spots (each having different Mw and isoelectric point) appeared as a few basic spots of lower Mw in the patient sample obtained 1 day after SP-HUS diagnosis (H837 sp1), and was recovered in the sample drawn 3 months later (H837 sp2). A similar situation applies to FHR-1. The small boxes within the gel images indicate the neuraminidase activity detected in samples sp1 and sp2 from patient H837.
Article Snippet: The anti-FH monoclonal antibodies (mAb) OX24 and C18 were from ThermoFisher (MA170057 and GAU0180302); the anti-FHR-1/FHR-2 mAb JHD7 was from Hycult Biotech (HM2301); the anti-FHR-4 mAb (MAB5980) and the anti-FHR-5 mAb (MAB3845) were from R&D.
Techniques: Purification, Two-Dimensional Gel Electrophoresis, Western Blot, Activity Assay