c3 protein  (Hycult Biotech)


Bioz Verified Symbol Hycult Biotech is a verified supplier
Bioz Manufacturer Symbol Hycult Biotech manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 92

    Structured Review

    Hycult Biotech c3 protein
    C3 Protein, supplied by Hycult Biotech, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/c3 protein/product/Hycult Biotech
    Average 92 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    c3 protein - by Bioz Stars, 2024-05
    92/100 stars

    Images

    c3 protein  (Hycult Biotech)


    Bioz Verified Symbol Hycult Biotech is a verified supplier
    Bioz Manufacturer Symbol Hycult Biotech manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 92

    Structured Review

    Hycult Biotech c3 protein
    C3 Protein, supplied by Hycult Biotech, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/c3 protein/product/Hycult Biotech
    Average 92 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    c3 protein - by Bioz Stars, 2024-05
    92/100 stars

    Images

    c3 protein  (Hycult Biotech)


    Bioz Verified Symbol Hycult Biotech is a verified supplier
    Bioz Manufacturer Symbol Hycult Biotech manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 92

    Structured Review

    Hycult Biotech c3 protein
    Overview of complement-related diseases.
    C3 Protein, supplied by Hycult Biotech, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/c3 protein/product/Hycult Biotech
    Average 92 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    c3 protein - by Bioz Stars, 2024-05
    92/100 stars

    Images

    1) Product Images from "Pitfalls in complement analysis: A systematic literature review of assessing complement activation"

    Article Title: Pitfalls in complement analysis: A systematic literature review of assessing complement activation

    Journal: Frontiers in Immunology

    doi: 10.3389/fimmu.2022.1007102

    Overview of complement-related diseases.
    Figure Legend Snippet: Overview of complement-related diseases.

    Techniques Used: Activation Assay, Enzyme-linked Immunosorbent Assay, Functional Assay, Infection, Binding Assay, Diagnostic Assay, Permeability, Chemotaxis Assay, Staining, Derivative Assay, Isolation, Marker, Activity Assay, Inhibition, Transplantation Assay, Biomarker Assay, Labeling, Variant Assay, RIA Assay, Immunoelectrophoresis

    Overview of the 45 selected studies that assessed complement components ex-vivo in the past 5 years (2017-2022).
    Figure Legend Snippet: Overview of the 45 selected studies that assessed complement components ex-vivo in the past 5 years (2017-2022).

    Techniques Used: Activation Assay, Enzyme-linked Immunosorbent Assay, Diagnostic Assay, Immunoturbidimetry Assay, Activity Assay, Ex Vivo, Radial Immuno Diffusion, Infection, Coagulation, Transplantation Assay, Purification, Rocket Immunoelectrophoresis, Permeability, Binding Assay

    c3b/ic3b, human, mab 3e7  (Hycult Biotech)


    Bioz Verified Symbol Hycult Biotech is a verified supplier
    Bioz Manufacturer Symbol Hycult Biotech manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 92

    Structured Review

    Hycult Biotech c3b/ic3b, human, mab 3e7
    C3b/Ic3b, Human, Mab 3e7, supplied by Hycult Biotech, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/c3b/ic3b, human, mab 3e7/product/Hycult Biotech
    Average 92 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    c3b/ic3b, human, mab 3e7 - by Bioz Stars, 2024-05
    92/100 stars

    Images

    human complement factor c3b ic3b  (Hycult Biotech)


    Bioz Verified Symbol Hycult Biotech is a verified supplier
    Bioz Manufacturer Symbol Hycult Biotech manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 92

    Structured Review

    Hycult Biotech human complement factor c3b ic3b
    Glycine-dependent mechanism for HtrE, NfrA, YhcD expression. a Relative ATP abundance of E. coli K12 in the presence or absence of 100 mM glycine for the indicated length of time ( n = 3). b Percent survival of E. coli K12 in the presence or absence of 5 mM ATP, 100 mM glycine or/and 100 µL serum ( n = 3). c Relative cAMP abundance of E. coli K12 in the presence or absence of 100 mM glycine or/and 100 µL serum ( n = 3). d Percent survival of the indicated mutants in the presence of 100 mM glycine, 100 µL serum or both ( n = 3). e Western blot of CRP in E. coli K12 exposed to 0–5 mM ATP or 0–4 mM AMP. f Effect of 100 mM glycine, 100 µL serum or both on CRP expression. g Western blot of HtrE, NfrA, YhcD in these mutants. h qRT-PCR of htrE , nfrA , yhcD in these mutants ( n = 3). i Flow cytometry quantification of anti-C9 neoantigen on outer membrane surface of the indicated mutants ( n = 3). j Percent survival of the indicated mutants in the presence of 100 mM glycine, 100 µL serum or both ( n = 3). k Western blot of HtrE, NfrA, YhcD of E. coli K12 in the presence of 100 mM glycine and the indicated amount of serum. l , m Western blot of HtrE, NfrA, YhcD of E. coli K12 ( l ) and Y17 ( m ) in the presence of 100 µL serum and the indicated concentrations of glycine. n Flow cytometry quantification of <t>anti-C3b</t> and anti-C9 neoantigen on E. coli K12 and Y17 in the presence or absence of 100 µL serum or 100 mM glycine or both. o , MST for the interaction of complement C3 with HtrE, NfrA, YhcD ( n = 3). i , n 50,000 cells were record with forwarding versus side scatter and were gated before data acquisition. Results in ( a – d , h – j , n , o ) are displayed as mean ± SEM, and significant differences are identified (* p < 0.05, ** p < 0.01) as determined by two-tailed Student’s t test. See also Supplementary Figs. –
    Human Complement Factor C3b Ic3b, supplied by Hycult Biotech, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/human complement factor c3b ic3b/product/Hycult Biotech
    Average 92 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    human complement factor c3b ic3b - by Bioz Stars, 2024-05
    92/100 stars

    Images

    1) Product Images from "Glycine, serine and threonine metabolism confounds efficacy of complement-mediated killing"

    Article Title: Glycine, serine and threonine metabolism confounds efficacy of complement-mediated killing

    Journal: Nature Communications

    doi: 10.1038/s41467-019-11129-5

    Glycine-dependent mechanism for HtrE, NfrA, YhcD expression. a Relative ATP abundance of E. coli K12 in the presence or absence of 100 mM glycine for the indicated length of time ( n = 3). b Percent survival of E. coli K12 in the presence or absence of 5 mM ATP, 100 mM glycine or/and 100 µL serum ( n = 3). c Relative cAMP abundance of E. coli K12 in the presence or absence of 100 mM glycine or/and 100 µL serum ( n = 3). d Percent survival of the indicated mutants in the presence of 100 mM glycine, 100 µL serum or both ( n = 3). e Western blot of CRP in E. coli K12 exposed to 0–5 mM ATP or 0–4 mM AMP. f Effect of 100 mM glycine, 100 µL serum or both on CRP expression. g Western blot of HtrE, NfrA, YhcD in these mutants. h qRT-PCR of htrE , nfrA , yhcD in these mutants ( n = 3). i Flow cytometry quantification of anti-C9 neoantigen on outer membrane surface of the indicated mutants ( n = 3). j Percent survival of the indicated mutants in the presence of 100 mM glycine, 100 µL serum or both ( n = 3). k Western blot of HtrE, NfrA, YhcD of E. coli K12 in the presence of 100 mM glycine and the indicated amount of serum. l , m Western blot of HtrE, NfrA, YhcD of E. coli K12 ( l ) and Y17 ( m ) in the presence of 100 µL serum and the indicated concentrations of glycine. n Flow cytometry quantification of anti-C3b and anti-C9 neoantigen on E. coli K12 and Y17 in the presence or absence of 100 µL serum or 100 mM glycine or both. o , MST for the interaction of complement C3 with HtrE, NfrA, YhcD ( n = 3). i , n 50,000 cells were record with forwarding versus side scatter and were gated before data acquisition. Results in ( a – d , h – j , n , o ) are displayed as mean ± SEM, and significant differences are identified (* p < 0.05, ** p < 0.01) as determined by two-tailed Student’s t test. See also Supplementary Figs. –
    Figure Legend Snippet: Glycine-dependent mechanism for HtrE, NfrA, YhcD expression. a Relative ATP abundance of E. coli K12 in the presence or absence of 100 mM glycine for the indicated length of time ( n = 3). b Percent survival of E. coli K12 in the presence or absence of 5 mM ATP, 100 mM glycine or/and 100 µL serum ( n = 3). c Relative cAMP abundance of E. coli K12 in the presence or absence of 100 mM glycine or/and 100 µL serum ( n = 3). d Percent survival of the indicated mutants in the presence of 100 mM glycine, 100 µL serum or both ( n = 3). e Western blot of CRP in E. coli K12 exposed to 0–5 mM ATP or 0–4 mM AMP. f Effect of 100 mM glycine, 100 µL serum or both on CRP expression. g Western blot of HtrE, NfrA, YhcD in these mutants. h qRT-PCR of htrE , nfrA , yhcD in these mutants ( n = 3). i Flow cytometry quantification of anti-C9 neoantigen on outer membrane surface of the indicated mutants ( n = 3). j Percent survival of the indicated mutants in the presence of 100 mM glycine, 100 µL serum or both ( n = 3). k Western blot of HtrE, NfrA, YhcD of E. coli K12 in the presence of 100 mM glycine and the indicated amount of serum. l , m Western blot of HtrE, NfrA, YhcD of E. coli K12 ( l ) and Y17 ( m ) in the presence of 100 µL serum and the indicated concentrations of glycine. n Flow cytometry quantification of anti-C3b and anti-C9 neoantigen on E. coli K12 and Y17 in the presence or absence of 100 µL serum or 100 mM glycine or both. o , MST for the interaction of complement C3 with HtrE, NfrA, YhcD ( n = 3). i , n 50,000 cells were record with forwarding versus side scatter and were gated before data acquisition. Results in ( a – d , h – j , n , o ) are displayed as mean ± SEM, and significant differences are identified (* p < 0.05, ** p < 0.01) as determined by two-tailed Student’s t test. See also Supplementary Figs. –

    Techniques Used: Expressing, Western Blot, Quantitative RT-PCR, Flow Cytometry, Two Tailed Test

    c3b c3 h2o  (Hycult Biotech)


    Bioz Verified Symbol Hycult Biotech is a verified supplier
    Bioz Manufacturer Symbol Hycult Biotech manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 92

    Structured Review

    Hycult Biotech c3b c3 h2o
    C3b C3 H2o, supplied by Hycult Biotech, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/c3b c3 h2o/product/Hycult Biotech
    Average 92 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    c3b c3 h2o - by Bioz Stars, 2024-05
    92/100 stars

    Images

    c3b c3 h2o  (Hycult Biotech)


    Bioz Verified Symbol Hycult Biotech is a verified supplier
    Bioz Manufacturer Symbol Hycult Biotech manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 92

    Structured Review

    Hycult Biotech c3b c3 h2o
    C3b C3 H2o, supplied by Hycult Biotech, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/c3b c3 h2o/product/Hycult Biotech
    Average 92 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    c3b c3 h2o - by Bioz Stars, 2024-05
    92/100 stars

    Images

    c3b c3 h2o  (Hycult Biotech)


    Bioz Verified Symbol Hycult Biotech is a verified supplier
    Bioz Manufacturer Symbol Hycult Biotech manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 92

    Structured Review

    Hycult Biotech c3b c3 h2o
    C3b C3 H2o, supplied by Hycult Biotech, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/c3b c3 h2o/product/Hycult Biotech
    Average 92 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    c3b c3 h2o - by Bioz Stars, 2024-05
    92/100 stars

    Images

    c3b c3 h2o  (Hycult Biotech)


    Bioz Verified Symbol Hycult Biotech is a verified supplier
    Bioz Manufacturer Symbol Hycult Biotech manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 92

    Structured Review

    Hycult Biotech c3b c3 h2o
    Decellularized transwells of ARPE-19 wildtype (left panels) and ARPE-19- EFEMP1 R345/R345W (right panels) cultures. Exposed ECM immunostained with antibodies for (A, B) <t>C3b/C3(H2O)</t> and (C,D) CFH. (E, F) Co-staining for C3b/C3(H2O) and CFH. Scale bars 50 m. (G) Average quantification of C3b/C3(H2O) and CFH fluorescent labeling comparing exposed ECMs. (Data represented as mean ± SD.*p<0.05).
    C3b C3 H2o, supplied by Hycult Biotech, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/c3b c3 h2o/product/Hycult Biotech
    Average 92 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    c3b c3 h2o - by Bioz Stars, 2024-05
    92/100 stars

    Images

    1) Product Images from "Changes in extracellular matrix cause RPE cells to make basal deposits and activate the alternative complement pathway"

    Article Title: Changes in extracellular matrix cause RPE cells to make basal deposits and activate the alternative complement pathway

    Journal: bioRxiv

    doi: 10.1101/152835

    Decellularized transwells of ARPE-19 wildtype (left panels) and ARPE-19- EFEMP1 R345/R345W (right panels) cultures. Exposed ECM immunostained with antibodies for (A, B) C3b/C3(H2O) and (C,D) CFH. (E, F) Co-staining for C3b/C3(H2O) and CFH. Scale bars 50 m. (G) Average quantification of C3b/C3(H2O) and CFH fluorescent labeling comparing exposed ECMs. (Data represented as mean ± SD.*p<0.05).
    Figure Legend Snippet: Decellularized transwells of ARPE-19 wildtype (left panels) and ARPE-19- EFEMP1 R345/R345W (right panels) cultures. Exposed ECM immunostained with antibodies for (A, B) C3b/C3(H2O) and (C,D) CFH. (E, F) Co-staining for C3b/C3(H2O) and CFH. Scale bars 50 m. (G) Average quantification of C3b/C3(H2O) and CFH fluorescent labeling comparing exposed ECMs. (Data represented as mean ± SD.*p<0.05).

    Techniques Used: Staining, Labeling

    HfRPE cultures grown on ECM made by ARPE-19 wildtype or ARPE-19- EFEMP1 R345/R345W were decellularized and immunostained with antibodies for (A, B) C3b/C3(H2O), (C, D) CFH or (E, F) both C3b/C3(H2O) and CFH. Scale bars: 50 μm. (G) C3a and (H) CFH measured by ELISA in apical and basal conditioned media of hfRPE cells grown on ECM made by ARPE-19 wildtype (ECM WT) or ARPE-19- EFEMP1 R345/R345W (ECM mut) in the absence of serum. (Data represented as mean ± SD. n=4 per type, *p<0.005, **p<0.001).
    Figure Legend Snippet: HfRPE cultures grown on ECM made by ARPE-19 wildtype or ARPE-19- EFEMP1 R345/R345W were decellularized and immunostained with antibodies for (A, B) C3b/C3(H2O), (C, D) CFH or (E, F) both C3b/C3(H2O) and CFH. Scale bars: 50 μm. (G) C3a and (H) CFH measured by ELISA in apical and basal conditioned media of hfRPE cells grown on ECM made by ARPE-19 wildtype (ECM WT) or ARPE-19- EFEMP1 R345/R345W (ECM mut) in the absence of serum. (Data represented as mean ± SD. n=4 per type, *p<0.005, **p<0.001).

    Techniques Used: Enzyme-linked Immunosorbent Assay

    Similar Products

  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 92
    Hycult Biotech c3 protein
    C3 Protein, supplied by Hycult Biotech, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/c3 protein/product/Hycult Biotech
    Average 92 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    c3 protein - by Bioz Stars, 2024-05
    92/100 stars
      Buy from Supplier

    92
    Hycult Biotech c3b/ic3b, human, mab 3e7
    C3b/Ic3b, Human, Mab 3e7, supplied by Hycult Biotech, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/c3b/ic3b, human, mab 3e7/product/Hycult Biotech
    Average 92 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    c3b/ic3b, human, mab 3e7 - by Bioz Stars, 2024-05
    92/100 stars
      Buy from Supplier

    92
    Hycult Biotech human complement factor c3b ic3b
    Glycine-dependent mechanism for HtrE, NfrA, YhcD expression. a Relative ATP abundance of E. coli K12 in the presence or absence of 100 mM glycine for the indicated length of time ( n = 3). b Percent survival of E. coli K12 in the presence or absence of 5 mM ATP, 100 mM glycine or/and 100 µL serum ( n = 3). c Relative cAMP abundance of E. coli K12 in the presence or absence of 100 mM glycine or/and 100 µL serum ( n = 3). d Percent survival of the indicated mutants in the presence of 100 mM glycine, 100 µL serum or both ( n = 3). e Western blot of CRP in E. coli K12 exposed to 0–5 mM ATP or 0–4 mM AMP. f Effect of 100 mM glycine, 100 µL serum or both on CRP expression. g Western blot of HtrE, NfrA, YhcD in these mutants. h qRT-PCR of htrE , nfrA , yhcD in these mutants ( n = 3). i Flow cytometry quantification of anti-C9 neoantigen on outer membrane surface of the indicated mutants ( n = 3). j Percent survival of the indicated mutants in the presence of 100 mM glycine, 100 µL serum or both ( n = 3). k Western blot of HtrE, NfrA, YhcD of E. coli K12 in the presence of 100 mM glycine and the indicated amount of serum. l , m Western blot of HtrE, NfrA, YhcD of E. coli K12 ( l ) and Y17 ( m ) in the presence of 100 µL serum and the indicated concentrations of glycine. n Flow cytometry quantification of <t>anti-C3b</t> and anti-C9 neoantigen on E. coli K12 and Y17 in the presence or absence of 100 µL serum or 100 mM glycine or both. o , MST for the interaction of complement C3 with HtrE, NfrA, YhcD ( n = 3). i , n 50,000 cells were record with forwarding versus side scatter and were gated before data acquisition. Results in ( a – d , h – j , n , o ) are displayed as mean ± SEM, and significant differences are identified (* p < 0.05, ** p < 0.01) as determined by two-tailed Student’s t test. See also Supplementary Figs. –
    Human Complement Factor C3b Ic3b, supplied by Hycult Biotech, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/human complement factor c3b ic3b/product/Hycult Biotech
    Average 92 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    human complement factor c3b ic3b - by Bioz Stars, 2024-05
    92/100 stars
      Buy from Supplier

    92
    Hycult Biotech c3b c3 h2o
    Glycine-dependent mechanism for HtrE, NfrA, YhcD expression. a Relative ATP abundance of E. coli K12 in the presence or absence of 100 mM glycine for the indicated length of time ( n = 3). b Percent survival of E. coli K12 in the presence or absence of 5 mM ATP, 100 mM glycine or/and 100 µL serum ( n = 3). c Relative cAMP abundance of E. coli K12 in the presence or absence of 100 mM glycine or/and 100 µL serum ( n = 3). d Percent survival of the indicated mutants in the presence of 100 mM glycine, 100 µL serum or both ( n = 3). e Western blot of CRP in E. coli K12 exposed to 0–5 mM ATP or 0–4 mM AMP. f Effect of 100 mM glycine, 100 µL serum or both on CRP expression. g Western blot of HtrE, NfrA, YhcD in these mutants. h qRT-PCR of htrE , nfrA , yhcD in these mutants ( n = 3). i Flow cytometry quantification of anti-C9 neoantigen on outer membrane surface of the indicated mutants ( n = 3). j Percent survival of the indicated mutants in the presence of 100 mM glycine, 100 µL serum or both ( n = 3). k Western blot of HtrE, NfrA, YhcD of E. coli K12 in the presence of 100 mM glycine and the indicated amount of serum. l , m Western blot of HtrE, NfrA, YhcD of E. coli K12 ( l ) and Y17 ( m ) in the presence of 100 µL serum and the indicated concentrations of glycine. n Flow cytometry quantification of <t>anti-C3b</t> and anti-C9 neoantigen on E. coli K12 and Y17 in the presence or absence of 100 µL serum or 100 mM glycine or both. o , MST for the interaction of complement C3 with HtrE, NfrA, YhcD ( n = 3). i , n 50,000 cells were record with forwarding versus side scatter and were gated before data acquisition. Results in ( a – d , h – j , n , o ) are displayed as mean ± SEM, and significant differences are identified (* p < 0.05, ** p < 0.01) as determined by two-tailed Student’s t test. See also Supplementary Figs. –
    C3b C3 H2o, supplied by Hycult Biotech, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/c3b c3 h2o/product/Hycult Biotech
    Average 92 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    c3b c3 h2o - by Bioz Stars, 2024-05
    92/100 stars
      Buy from Supplier

    Image Search Results


    Glycine-dependent mechanism for HtrE, NfrA, YhcD expression. a Relative ATP abundance of E. coli K12 in the presence or absence of 100 mM glycine for the indicated length of time ( n = 3). b Percent survival of E. coli K12 in the presence or absence of 5 mM ATP, 100 mM glycine or/and 100 µL serum ( n = 3). c Relative cAMP abundance of E. coli K12 in the presence or absence of 100 mM glycine or/and 100 µL serum ( n = 3). d Percent survival of the indicated mutants in the presence of 100 mM glycine, 100 µL serum or both ( n = 3). e Western blot of CRP in E. coli K12 exposed to 0–5 mM ATP or 0–4 mM AMP. f Effect of 100 mM glycine, 100 µL serum or both on CRP expression. g Western blot of HtrE, NfrA, YhcD in these mutants. h qRT-PCR of htrE , nfrA , yhcD in these mutants ( n = 3). i Flow cytometry quantification of anti-C9 neoantigen on outer membrane surface of the indicated mutants ( n = 3). j Percent survival of the indicated mutants in the presence of 100 mM glycine, 100 µL serum or both ( n = 3). k Western blot of HtrE, NfrA, YhcD of E. coli K12 in the presence of 100 mM glycine and the indicated amount of serum. l , m Western blot of HtrE, NfrA, YhcD of E. coli K12 ( l ) and Y17 ( m ) in the presence of 100 µL serum and the indicated concentrations of glycine. n Flow cytometry quantification of anti-C3b and anti-C9 neoantigen on E. coli K12 and Y17 in the presence or absence of 100 µL serum or 100 mM glycine or both. o , MST for the interaction of complement C3 with HtrE, NfrA, YhcD ( n = 3). i , n 50,000 cells were record with forwarding versus side scatter and were gated before data acquisition. Results in ( a – d , h – j , n , o ) are displayed as mean ± SEM, and significant differences are identified (* p < 0.05, ** p < 0.01) as determined by two-tailed Student’s t test. See also Supplementary Figs. –

    Journal: Nature Communications

    Article Title: Glycine, serine and threonine metabolism confounds efficacy of complement-mediated killing

    doi: 10.1038/s41467-019-11129-5

    Figure Lengend Snippet: Glycine-dependent mechanism for HtrE, NfrA, YhcD expression. a Relative ATP abundance of E. coli K12 in the presence or absence of 100 mM glycine for the indicated length of time ( n = 3). b Percent survival of E. coli K12 in the presence or absence of 5 mM ATP, 100 mM glycine or/and 100 µL serum ( n = 3). c Relative cAMP abundance of E. coli K12 in the presence or absence of 100 mM glycine or/and 100 µL serum ( n = 3). d Percent survival of the indicated mutants in the presence of 100 mM glycine, 100 µL serum or both ( n = 3). e Western blot of CRP in E. coli K12 exposed to 0–5 mM ATP or 0–4 mM AMP. f Effect of 100 mM glycine, 100 µL serum or both on CRP expression. g Western blot of HtrE, NfrA, YhcD in these mutants. h qRT-PCR of htrE , nfrA , yhcD in these mutants ( n = 3). i Flow cytometry quantification of anti-C9 neoantigen on outer membrane surface of the indicated mutants ( n = 3). j Percent survival of the indicated mutants in the presence of 100 mM glycine, 100 µL serum or both ( n = 3). k Western blot of HtrE, NfrA, YhcD of E. coli K12 in the presence of 100 mM glycine and the indicated amount of serum. l , m Western blot of HtrE, NfrA, YhcD of E. coli K12 ( l ) and Y17 ( m ) in the presence of 100 µL serum and the indicated concentrations of glycine. n Flow cytometry quantification of anti-C3b and anti-C9 neoantigen on E. coli K12 and Y17 in the presence or absence of 100 µL serum or 100 mM glycine or both. o , MST for the interaction of complement C3 with HtrE, NfrA, YhcD ( n = 3). i , n 50,000 cells were record with forwarding versus side scatter and were gated before data acquisition. Results in ( a – d , h – j , n , o ) are displayed as mean ± SEM, and significant differences are identified (* p < 0.05, ** p < 0.01) as determined by two-tailed Student’s t test. See also Supplementary Figs. –

    Article Snippet: Fluorescein isothiocyanate (FITC) conjugated monoclonal antibody to human complement factor C3b/iC3b (Cat. HM2286, clone 3E7) or a C9 neoantigen of the terminal complement complex (TCC) (Cat.HM2167F, clone aE11) were from Hycult Biotech Inc., PA, USA.

    Techniques: Expressing, Western Blot, Quantitative RT-PCR, Flow Cytometry, Two Tailed Test