masp-2/map19, human, mab 6g12  (Hycult Biotech)


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    Hycult Biotech masp-2/map19, human, mab 6g12
    Masp 2/Map19, Human, Mab 6g12, supplied by Hycult Biotech, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/masp-2/map19, human, mab 6g12/product/Hycult Biotech
    Average 90 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    masp-2/map19, human, mab 6g12 - by Bioz Stars, 2024-05
    90/100 stars

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    masp-2/map19, human, mab 6g12  (Hycult Biotech)


    Bioz Verified Symbol Hycult Biotech is a verified supplier
    Bioz Manufacturer Symbol Hycult Biotech manufactures this product  
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  • 90

    Structured Review

    Hycult Biotech masp-2/map19, human, mab 6g12
    Masp 2/Map19, Human, Mab 6g12, supplied by Hycult Biotech, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/masp-2/map19, human, mab 6g12/product/Hycult Biotech
    Average 90 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    masp-2/map19, human, mab 6g12 - by Bioz Stars, 2024-05
    90/100 stars

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    rat monoclonal anti human masp  (Hycult Biotech)


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    Hycult Biotech rat monoclonal anti human masp
    Analysis of ficolin-3 preparation (1 μg of final product) fractionated on a 10% polyacrylamide gel under reducing (lanes 1, 2, 5–8) or non-reducing (lanes 3–4) conditions. Coomassie Blue stain (lanes 1, 3) and Western blotting using anti-ficolin-3 (lanes 2, 4), anti-MASP-1 (lane 5), <t>anti-MASP-2</t> (lane 6), and anti-MASP-3 (lane 7) antibodies. The last lane (8) is the strip 4 re-probed with anti-human IgG, IgA and IgM. MASP bands are marked with asterisks (*). A, b, c–heavy chains of IgM (a), IgA (b), IgG (c); d- ficolin-3 monomer; e- light chains of Ig. The positions of the molecular mass markers are indicated in kDa.
    Rat Monoclonal Anti Human Masp, supplied by Hycult Biotech, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rat monoclonal anti human masp/product/Hycult Biotech
    Average 90 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    rat monoclonal anti human masp - by Bioz Stars, 2024-05
    90/100 stars

    Images

    1) Product Images from "A New Ligand-Based Method for Purifying Active Human Plasma-Derived Ficolin-3 Complexes Supports the Phenomenon of Crosstalk between Pattern-Recognition Molecules and Immunoglobulins"

    Article Title: A New Ligand-Based Method for Purifying Active Human Plasma-Derived Ficolin-3 Complexes Supports the Phenomenon of Crosstalk between Pattern-Recognition Molecules and Immunoglobulins

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0156691

    Analysis of ficolin-3 preparation (1 μg of final product) fractionated on a 10% polyacrylamide gel under reducing (lanes 1, 2, 5–8) or non-reducing (lanes 3–4) conditions. Coomassie Blue stain (lanes 1, 3) and Western blotting using anti-ficolin-3 (lanes 2, 4), anti-MASP-1 (lane 5), anti-MASP-2 (lane 6), and anti-MASP-3 (lane 7) antibodies. The last lane (8) is the strip 4 re-probed with anti-human IgG, IgA and IgM. MASP bands are marked with asterisks (*). A, b, c–heavy chains of IgM (a), IgA (b), IgG (c); d- ficolin-3 monomer; e- light chains of Ig. The positions of the molecular mass markers are indicated in kDa.
    Figure Legend Snippet: Analysis of ficolin-3 preparation (1 μg of final product) fractionated on a 10% polyacrylamide gel under reducing (lanes 1, 2, 5–8) or non-reducing (lanes 3–4) conditions. Coomassie Blue stain (lanes 1, 3) and Western blotting using anti-ficolin-3 (lanes 2, 4), anti-MASP-1 (lane 5), anti-MASP-2 (lane 6), and anti-MASP-3 (lane 7) antibodies. The last lane (8) is the strip 4 re-probed with anti-human IgG, IgA and IgM. MASP bands are marked with asterisks (*). A, b, c–heavy chains of IgM (a), IgA (b), IgG (c); d- ficolin-3 monomer; e- light chains of Ig. The positions of the molecular mass markers are indicated in kDa.

    Techniques Used: Staining, Western Blot, Stripping Membranes

    (A) MASP-1 activity is expressed as the fluorescence of a product emerging due to the capacity of MASP-1 to cleave VPR-AMC substrate. (B) MASP-2 activity was determined by detection of C4 activation products deposition assay by specific antibodies. Conditions excluding activation of complement classical pathway were applied (Petersen et al., 2001).
    Figure Legend Snippet: (A) MASP-1 activity is expressed as the fluorescence of a product emerging due to the capacity of MASP-1 to cleave VPR-AMC substrate. (B) MASP-2 activity was determined by detection of C4 activation products deposition assay by specific antibodies. Conditions excluding activation of complement classical pathway were applied (Petersen et al., 2001).

    Techniques Used: Activity Assay, Fluorescence, Activation Assay

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    Hycult Biotech masp-2/map19, human, mab 6g12
    Masp 2/Map19, Human, Mab 6g12, supplied by Hycult Biotech, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/masp-2/map19, human, mab 6g12/product/Hycult Biotech
    Average 90 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    masp-2/map19, human, mab 6g12 - by Bioz Stars, 2024-05
    90/100 stars
      Buy from Supplier

    90
    Hycult Biotech rat monoclonal anti human masp
    Analysis of ficolin-3 preparation (1 μg of final product) fractionated on a 10% polyacrylamide gel under reducing (lanes 1, 2, 5–8) or non-reducing (lanes 3–4) conditions. Coomassie Blue stain (lanes 1, 3) and Western blotting using anti-ficolin-3 (lanes 2, 4), anti-MASP-1 (lane 5), <t>anti-MASP-2</t> (lane 6), and anti-MASP-3 (lane 7) antibodies. The last lane (8) is the strip 4 re-probed with anti-human IgG, IgA and IgM. MASP bands are marked with asterisks (*). A, b, c–heavy chains of IgM (a), IgA (b), IgG (c); d- ficolin-3 monomer; e- light chains of Ig. The positions of the molecular mass markers are indicated in kDa.
    Rat Monoclonal Anti Human Masp, supplied by Hycult Biotech, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rat monoclonal anti human masp/product/Hycult Biotech
    Average 90 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    rat monoclonal anti human masp - by Bioz Stars, 2024-05
    90/100 stars
      Buy from Supplier

    Image Search Results


    Analysis of ficolin-3 preparation (1 μg of final product) fractionated on a 10% polyacrylamide gel under reducing (lanes 1, 2, 5–8) or non-reducing (lanes 3–4) conditions. Coomassie Blue stain (lanes 1, 3) and Western blotting using anti-ficolin-3 (lanes 2, 4), anti-MASP-1 (lane 5), anti-MASP-2 (lane 6), and anti-MASP-3 (lane 7) antibodies. The last lane (8) is the strip 4 re-probed with anti-human IgG, IgA and IgM. MASP bands are marked with asterisks (*). A, b, c–heavy chains of IgM (a), IgA (b), IgG (c); d- ficolin-3 monomer; e- light chains of Ig. The positions of the molecular mass markers are indicated in kDa.

    Journal: PLoS ONE

    Article Title: A New Ligand-Based Method for Purifying Active Human Plasma-Derived Ficolin-3 Complexes Supports the Phenomenon of Crosstalk between Pattern-Recognition Molecules and Immunoglobulins

    doi: 10.1371/journal.pone.0156691

    Figure Lengend Snippet: Analysis of ficolin-3 preparation (1 μg of final product) fractionated on a 10% polyacrylamide gel under reducing (lanes 1, 2, 5–8) or non-reducing (lanes 3–4) conditions. Coomassie Blue stain (lanes 1, 3) and Western blotting using anti-ficolin-3 (lanes 2, 4), anti-MASP-1 (lane 5), anti-MASP-2 (lane 6), and anti-MASP-3 (lane 7) antibodies. The last lane (8) is the strip 4 re-probed with anti-human IgG, IgA and IgM. MASP bands are marked with asterisks (*). A, b, c–heavy chains of IgM (a), IgA (b), IgG (c); d- ficolin-3 monomer; e- light chains of Ig. The positions of the molecular mass markers are indicated in kDa.

    Article Snippet: After blocking for 2 h with 5% skim milk, membranes were incubated with goat polyclonal anti-MASP-1 (catalogue number: sc-50841, Santa Cruz Biotechnology), rat monoclonal anti-human MASP-2/MAp-19 (Hycult Biotech, catalogue number: HM2191), or goat polyclonal anti-complement MASP-3 (catalogue number: AF1724-SP, R&D Systems) for 2 h at room temperature.

    Techniques: Staining, Western Blot, Stripping Membranes

    (A) MASP-1 activity is expressed as the fluorescence of a product emerging due to the capacity of MASP-1 to cleave VPR-AMC substrate. (B) MASP-2 activity was determined by detection of C4 activation products deposition assay by specific antibodies. Conditions excluding activation of complement classical pathway were applied (Petersen et al., 2001).

    Journal: PLoS ONE

    Article Title: A New Ligand-Based Method for Purifying Active Human Plasma-Derived Ficolin-3 Complexes Supports the Phenomenon of Crosstalk between Pattern-Recognition Molecules and Immunoglobulins

    doi: 10.1371/journal.pone.0156691

    Figure Lengend Snippet: (A) MASP-1 activity is expressed as the fluorescence of a product emerging due to the capacity of MASP-1 to cleave VPR-AMC substrate. (B) MASP-2 activity was determined by detection of C4 activation products deposition assay by specific antibodies. Conditions excluding activation of complement classical pathway were applied (Petersen et al., 2001).

    Article Snippet: After blocking for 2 h with 5% skim milk, membranes were incubated with goat polyclonal anti-MASP-1 (catalogue number: sc-50841, Santa Cruz Biotechnology), rat monoclonal anti-human MASP-2/MAp-19 (Hycult Biotech, catalogue number: HM2191), or goat polyclonal anti-complement MASP-3 (catalogue number: AF1724-SP, R&D Systems) for 2 h at room temperature.

    Techniques: Activity Assay, Fluorescence, Activation Assay