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human c5b 9  (Hycult Biotech)


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    Hycult Biotech human c5b 9
    Representative images from immunofluorescent double staining of factor H-related protein 3 (FHR-3) with (A-D) C4d, (E-H) C3d, and (I-L) <t>C5b-9</t> in kidney allografts prior to transplantation from organ donors after brain death (n=3 per staining). Nuclei were counterstained with DAPI (blue in A, E, and I). Complement deposition was stained with fluorescein isothiocyanate (FITC)-conjugated antibodies (green in B, F, and J), and FHR-3 was visualized using the TSA tetramethylrhodamine (TRITC) System (red in C, G, and K). Double stainings (D, H, and L) revealed partial glomerular colocalization of FHR-3 with C4d and C3d but not with C5b-9 in kidneys from deceased organ donors prior to transplantation.
    Human C5b 9, supplied by Hycult Biotech, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/human c5b 9/product/Hycult Biotech
    Average 92 stars, based on 1 article reviews
    human c5b 9 - by Bioz Stars, 2025-05
    92/100 stars

    Images

    1) Product Images from "Factor H-related protein 3 (FHR-3) deposition in kidney allografts – localization and correlation with complement activation"

    Article Title: Factor H-related protein 3 (FHR-3) deposition in kidney allografts – localization and correlation with complement activation

    Journal: medRxiv

    doi: 10.1101/2023.10.24.23297478

    Representative images from immunofluorescent double staining of factor H-related protein 3 (FHR-3) with (A-D) C4d, (E-H) C3d, and (I-L) C5b-9 in kidney allografts prior to transplantation from organ donors after brain death (n=3 per staining). Nuclei were counterstained with DAPI (blue in A, E, and I). Complement deposition was stained with fluorescein isothiocyanate (FITC)-conjugated antibodies (green in B, F, and J), and FHR-3 was visualized using the TSA tetramethylrhodamine (TRITC) System (red in C, G, and K). Double stainings (D, H, and L) revealed partial glomerular colocalization of FHR-3 with C4d and C3d but not with C5b-9 in kidneys from deceased organ donors prior to transplantation.
    Figure Legend Snippet: Representative images from immunofluorescent double staining of factor H-related protein 3 (FHR-3) with (A-D) C4d, (E-H) C3d, and (I-L) C5b-9 in kidney allografts prior to transplantation from organ donors after brain death (n=3 per staining). Nuclei were counterstained with DAPI (blue in A, E, and I). Complement deposition was stained with fluorescein isothiocyanate (FITC)-conjugated antibodies (green in B, F, and J), and FHR-3 was visualized using the TSA tetramethylrhodamine (TRITC) System (red in C, G, and K). Double stainings (D, H, and L) revealed partial glomerular colocalization of FHR-3 with C4d and C3d but not with C5b-9 in kidneys from deceased organ donors prior to transplantation.

    Techniques Used: Double Staining, Transplantation Assay, Staining



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    Representative images from immunofluorescent double staining of factor H-related protein 3 (FHR-3) with (A-D) C4d, (E-H) C3d, and (I-L) <t>C5b-9</t> in kidney allografts prior to transplantation from organ donors after brain death (n=3 per staining). Nuclei were counterstained with DAPI (blue in A, E, and I). Complement deposition was stained with fluorescein isothiocyanate (FITC)-conjugated antibodies (green in B, F, and J), and FHR-3 was visualized using the TSA tetramethylrhodamine (TRITC) System (red in C, G, and K). Double stainings (D, H, and L) revealed partial glomerular colocalization of FHR-3 with C4d and C3d but not with C5b-9 in kidneys from deceased organ donors prior to transplantation.
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    Representative images from immunofluorescent double staining of factor H-related protein 3 (FHR-3) with (A-D) C4d, (E-H) C3d, and (I-L) <t>C5b-9</t> in kidney allografts prior to transplantation from organ donors after brain death (n=3 per staining). Nuclei were counterstained with DAPI (blue in A, E, and I). Complement deposition was stained with fluorescein isothiocyanate (FITC)-conjugated antibodies (green in B, F, and J), and FHR-3 was visualized using the TSA tetramethylrhodamine (TRITC) System (red in C, G, and K). Double stainings (D, H, and L) revealed partial glomerular colocalization of FHR-3 with C4d and C3d but not with C5b-9 in kidneys from deceased organ donors prior to transplantation.
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    Representative images from immunofluorescent double staining of factor H-related protein 3 (FHR-3) with (A-D) C4d, (E-H) C3d, and (I-L) <t>C5b-9</t> in kidney allografts prior to transplantation from organ donors after brain death (n=3 per staining). Nuclei were counterstained with DAPI (blue in A, E, and I). Complement deposition was stained with fluorescein isothiocyanate (FITC)-conjugated antibodies (green in B, F, and J), and FHR-3 was visualized using the TSA tetramethylrhodamine (TRITC) System (red in C, G, and K). Double stainings (D, H, and L) revealed partial glomerular colocalization of FHR-3 with C4d and C3d but not with C5b-9 in kidneys from deceased organ donors prior to transplantation.
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    Representative images from immunofluorescent double staining of factor H-related protein 3 (FHR-3) with (A-D) C4d, (E-H) C3d, and (I-L) <t>C5b-9</t> in kidney allografts prior to transplantation from organ donors after brain death (n=3 per staining). Nuclei were counterstained with DAPI (blue in A, E, and I). Complement deposition was stained with fluorescein isothiocyanate (FITC)-conjugated antibodies (green in B, F, and J), and FHR-3 was visualized using the TSA tetramethylrhodamine (TRITC) System (red in C, G, and K). Double stainings (D, H, and L) revealed partial glomerular colocalization of FHR-3 with C4d and C3d but not with C5b-9 in kidneys from deceased organ donors prior to transplantation.
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    ( A–C ) CHC coating of PAEC reduces complement deposition, cellular activation and coagulation induced by human plasma. Uncoated and CHC-coated PAEC were treated for 4 hrs with 10% human plasma, and assessed by ELISA for ( A ) the deposition of activated complement <t>C5b-9</t> and ( B ) the expression of the adhesion molecule E-selectin ( B ). ( C ) Supernatants from this assay were measured for D-dimers concentration. CHC coating significantly protected WT PAEC. Uncoated GTKO.hCD46.hTBM PAEC were significantly protected compared to uncoated WT PAEC; coating with CHC further reduced complement deposition and cellular activation, although this was not statistically significant. ( D,F ) CHC coating of hTNFα-activated PAEC reduces complement deposition, cellular activation and coagulation induced by human plasma. WT and GTKO.hCD46.hTBM PAEC were treated with hTNFα (100 ng/ml) for 2 hrs to induce cellular activation and shedding of the endothelial glycocalyx. After, PAEC were coated with CHC and incubated for 4 hrs with 10% human plasma, and assessed for ( D ) complement C5b-9 deposition and ( E ) endothelial E-selectin expression as well as ( F ) D-dimer levels in the supernatants. CHC coating significantly protected hTNFα-activated WT and GTKO.hCD46.hTBM PAEC compared to uncoated PAEC. Significance was measured by one-way ANOVA with Bonferroni correction (*p < 0.05, **p < 0.01, ***p < 0.001). Data are mean ± SD of three independent experiments.
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    Image Search Results


    Representative images from immunofluorescent double staining of factor H-related protein 3 (FHR-3) with (A-D) C4d, (E-H) C3d, and (I-L) C5b-9 in kidney allografts prior to transplantation from organ donors after brain death (n=3 per staining). Nuclei were counterstained with DAPI (blue in A, E, and I). Complement deposition was stained with fluorescein isothiocyanate (FITC)-conjugated antibodies (green in B, F, and J), and FHR-3 was visualized using the TSA tetramethylrhodamine (TRITC) System (red in C, G, and K). Double stainings (D, H, and L) revealed partial glomerular colocalization of FHR-3 with C4d and C3d but not with C5b-9 in kidneys from deceased organ donors prior to transplantation.

    Journal: medRxiv

    Article Title: Factor H-related protein 3 (FHR-3) deposition in kidney allografts – localization and correlation with complement activation

    doi: 10.1101/2023.10.24.23297478

    Figure Lengend Snippet: Representative images from immunofluorescent double staining of factor H-related protein 3 (FHR-3) with (A-D) C4d, (E-H) C3d, and (I-L) C5b-9 in kidney allografts prior to transplantation from organ donors after brain death (n=3 per staining). Nuclei were counterstained with DAPI (blue in A, E, and I). Complement deposition was stained with fluorescein isothiocyanate (FITC)-conjugated antibodies (green in B, F, and J), and FHR-3 was visualized using the TSA tetramethylrhodamine (TRITC) System (red in C, G, and K). Double stainings (D, H, and L) revealed partial glomerular colocalization of FHR-3 with C4d and C3d but not with C5b-9 in kidneys from deceased organ donors prior to transplantation.

    Article Snippet: Complement deposition was detected using a rabbit polyclonal antibody (pAb) to human C4d (BI-RC4D, Biomedical), a rabbit pAb to human C3d (A0063, Dako), and a FITC-labeled mouse mAb to human C5b-9, clone aE11 (HM2167F, Hycult).

    Techniques: Double Staining, Transplantation Assay, Staining

    ( A–C ) CHC coating of PAEC reduces complement deposition, cellular activation and coagulation induced by human plasma. Uncoated and CHC-coated PAEC were treated for 4 hrs with 10% human plasma, and assessed by ELISA for ( A ) the deposition of activated complement C5b-9 and ( B ) the expression of the adhesion molecule E-selectin ( B ). ( C ) Supernatants from this assay were measured for D-dimers concentration. CHC coating significantly protected WT PAEC. Uncoated GTKO.hCD46.hTBM PAEC were significantly protected compared to uncoated WT PAEC; coating with CHC further reduced complement deposition and cellular activation, although this was not statistically significant. ( D,F ) CHC coating of hTNFα-activated PAEC reduces complement deposition, cellular activation and coagulation induced by human plasma. WT and GTKO.hCD46.hTBM PAEC were treated with hTNFα (100 ng/ml) for 2 hrs to induce cellular activation and shedding of the endothelial glycocalyx. After, PAEC were coated with CHC and incubated for 4 hrs with 10% human plasma, and assessed for ( D ) complement C5b-9 deposition and ( E ) endothelial E-selectin expression as well as ( F ) D-dimer levels in the supernatants. CHC coating significantly protected hTNFα-activated WT and GTKO.hCD46.hTBM PAEC compared to uncoated PAEC. Significance was measured by one-way ANOVA with Bonferroni correction (*p < 0.05, **p < 0.01, ***p < 0.001). Data are mean ± SD of three independent experiments.

    Journal: Scientific Reports

    Article Title: Surface modification of pig endothelial cells with a branched heparin conjugate improves their compatibility with human blood

    doi: 10.1038/s41598-017-04898-w

    Figure Lengend Snippet: ( A–C ) CHC coating of PAEC reduces complement deposition, cellular activation and coagulation induced by human plasma. Uncoated and CHC-coated PAEC were treated for 4 hrs with 10% human plasma, and assessed by ELISA for ( A ) the deposition of activated complement C5b-9 and ( B ) the expression of the adhesion molecule E-selectin ( B ). ( C ) Supernatants from this assay were measured for D-dimers concentration. CHC coating significantly protected WT PAEC. Uncoated GTKO.hCD46.hTBM PAEC were significantly protected compared to uncoated WT PAEC; coating with CHC further reduced complement deposition and cellular activation, although this was not statistically significant. ( D,F ) CHC coating of hTNFα-activated PAEC reduces complement deposition, cellular activation and coagulation induced by human plasma. WT and GTKO.hCD46.hTBM PAEC were treated with hTNFα (100 ng/ml) for 2 hrs to induce cellular activation and shedding of the endothelial glycocalyx. After, PAEC were coated with CHC and incubated for 4 hrs with 10% human plasma, and assessed for ( D ) complement C5b-9 deposition and ( E ) endothelial E-selectin expression as well as ( F ) D-dimer levels in the supernatants. CHC coating significantly protected hTNFα-activated WT and GTKO.hCD46.hTBM PAEC compared to uncoated PAEC. Significance was measured by one-way ANOVA with Bonferroni correction (*p < 0.05, **p < 0.01, ***p < 0.001). Data are mean ± SD of three independent experiments.

    Article Snippet: Rabbit anti-human C3b/c FITC (A0062; Dako), mouse anti-human C5b-9 FITC (clone aE11; HM2167F, Hycult Biotech), and mouse anti-human CD62E FITC (clone 1.2B6, MCA883F, AbD Serotec) were diluted in PBS/1% BSA and incubated for 60 min at RT followed by three washes.

    Techniques: Activation Assay, Coagulation, Enzyme-linked Immunosorbent Assay, Expressing, Concentration Assay, Incubation