hm2167  (Hycult Biotech)

Journal: iScience

Article Title: The immune landscape of SARS-CoV-2-associated Multisystem Inflammatory Syndrome in Children (MIS-C) from acute disease to recovery

doi: 10.1016/j.isci.2021.103215

Figure Lengend Snippet:

Article Snippet: Mouse anti-TCC (aE11) , Hycult , Cat#HM2167-1MG.

Techniques: Purification, Recombinant, Binding Assay, Blocking Assay, Staining, Enzyme-linked Immunosorbent Assay, Multiplex Assay, Software, Cytometry

Journal: Human Molecular Genetics

Article Title: The rare C9 P167S risk variant for age-related macular degeneration increases polymerization of the terminal component of the complement cascade

doi: 10.1093/hmg/ddab086

Figure Lengend Snippet: Surface plasmon resonance analysis of polymerization. Preparations of C9 were polished by size exchange chromatography to remove aggregates and flowed slowly (5 μl/min) at 37°C across a Biacore chip that had been immobilized with aE11 mAb specific for polymerized C9. Polymers of C9 formed by WT and P167S C9 were captured on the antibody. Solid line represents poly-C9 captured from the preparation of mutant P167S C9 and dashed line represents capture of poly-C9 in the WT preparation. The experiment was performed multiple times with different preparations of C9; a representative analysis is illustrated.

Article Snippet: Flat-bottomed 96-well plates (Maxisorp, ThermoFisher) were coated with 0.5 μg/ml human terminal complement complex-specific mAb aE11 (Hycult, HM2167, Uden, The Netherlands) in 200 m m bicarbonate/carbonate buffer pH 9.6 and incubated overnight at 4°C.

Techniques: SPR Assay, Chromatography, Mutagenesis