Journal: Pathogens
Article Title: Velocity Gradient Separation Reveals a New Extracellular Vesicle Population Enriched in miR-155 and Mitochondrial DNA
doi: 10.3390/pathogens10050526
Figure Lengend Snippet: Separation of plasma EVs and calprotectin by density or velocity gradients. Plasma EVs from two HIV-1 negative individuals were precipitated by ExoQuick™ before overlaid on sucrose density ( A , B ), iodixanol density ( C , D ), or iodixanol velocity ( E , F ) gradients. AChE (left panels) and calprotectin (right panels) in each gradient fractions were respectively measured by enzymatic assay and ELISA.
Article Snippet: Monoclonal antibody specific for human calprotectin (mAb 27E10) was purchased from Hycult ® Biotech (Plymouth Meeting, PA, USA).
Techniques: Enzymatic Assay, Enzyme-linked Immunosorbent Assay