Journal:
Article Title: Scavenger Receptor Class B Is Required for Hepatitis C Virus Uptake and Cross-Presentation by Human Dendritic Cells
doi: 10.1128/JVI.02478-07
Figure Lengend Snippet: SR expression on DCs and other cell types. Cell surface expression of SR was determined by flow cytometry using antibodies directed against SR-BI, CD36, LOX-1, or control antibody and preimmune serum. In addition, cells were stained for CD81 expression using a monoclonal anti-human CD81 antibody. Histograms corresponding to cell surface expression of the respective cell surface molecules (open curves) are overlaid with histograms of cells incubated with the appropriate isotype control (gray-shaded curves [NC]). FL2-H, fluorescence 2-height.
Article Snippet: Anti-human CD36 (FA6-152) MAb was obtained from Immunotech (Marseille, France), anti-human LOX-1 (23C11) MAb from HyCult Biotechnology (Uden, The Netherlands), anti-human CD81 (JS81) from BD Pharmingen (San Jose, CA), and rabbit anti-SR-BI (NB 400-104) and anti-SR-BII (NB 400-102) polyclonal sera from Novus Biologicals (Littleton, CO).
Techniques: Expressing, Flow Cytometry, Staining, Incubation, Fluorescence