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mouse monoclonal anti fhl2  (Hycult Biotech)


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    Structured Review

    Hycult Biotech mouse monoclonal anti fhl2
    Primer sequences for quantitative real-time PCR.
    Mouse Monoclonal Anti Fhl2, supplied by Hycult Biotech, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mouse monoclonal anti fhl2/product/Hycult Biotech
    Average 90 stars, based on 1 article reviews
    mouse monoclonal anti fhl2 - by Bioz Stars, 2025-05
    90/100 stars

    Images

    1) Product Images from "Four-And-A-Half LIM-Domain Protein 2 (FHL2) Deficiency Aggravates Cholestatic Liver Injury"

    Article Title: Four-And-A-Half LIM-Domain Protein 2 (FHL2) Deficiency Aggravates Cholestatic Liver Injury

    Journal: Cells

    doi: 10.3390/cells9010248

    Primer sequences for quantitative real-time PCR.
    Figure Legend Snippet: Primer sequences for quantitative real-time PCR.

    Techniques Used:

    Fhl2 expression and effect of Fhl2 deficiency on hepatocellular injury and inflammation in the mouse model of bile duct ligation (BDL). Fhl2 -deficient ( Fhl2 -ko) and wild type (wt) mice were either bile duct ligated (BDL) or sham-operated (CTR). ( A ) Fhl2 mRNA levels in wt BDL and CTR mice analyzed by qRT-PCR. ( B ) Representative hematoxylin and eosin stainings of liver tissue samples (20× magnification). ( C ) ALT (alanine aminotransferase) and ( D ) bilirubin serum levels. ( E ) Hmox-1 and ( F ) Mcp-1 mRNA expression levels in liver tissue analyzed by qRT-PCR. ( G ) Immunohistochemical CD3 staining of liver tissue samples (20× magnification). ( H ) Il-1 and ( I ) Tnf mRNA expression levels in liver tissue analyzed by qRT-PCR. (*: p < 0.05).
    Figure Legend Snippet: Fhl2 expression and effect of Fhl2 deficiency on hepatocellular injury and inflammation in the mouse model of bile duct ligation (BDL). Fhl2 -deficient ( Fhl2 -ko) and wild type (wt) mice were either bile duct ligated (BDL) or sham-operated (CTR). ( A ) Fhl2 mRNA levels in wt BDL and CTR mice analyzed by qRT-PCR. ( B ) Representative hematoxylin and eosin stainings of liver tissue samples (20× magnification). ( C ) ALT (alanine aminotransferase) and ( D ) bilirubin serum levels. ( E ) Hmox-1 and ( F ) Mcp-1 mRNA expression levels in liver tissue analyzed by qRT-PCR. ( G ) Immunohistochemical CD3 staining of liver tissue samples (20× magnification). ( H ) Il-1 and ( I ) Tnf mRNA expression levels in liver tissue analyzed by qRT-PCR. (*: p < 0.05).

    Techniques Used: Expressing, Ligation, Quantitative RT-PCR, Immunohistochemical staining, Staining

    Effect of Fhl2 deficiency on hepatic fibrosis in the mouse model of bile duct ligation (BDL). Fhl2 -deficient ( Fhl2 -ko) and wild type (wt) mice were either subjected to BDL or were sham-operated (CTR). ( A ) Hepatic α-sma mRNA expression levels. ( B ) Immunohistochemical α-sma staining of liver tissue sections (20× magnification). Hepatic ( C ) Tgf-β and ( D ) Col1a1 mRNA expression levels. (E) Sirius Red/Fast Green staining of liver tissue sections (20× magnification). Hepatic ( F ) Mmp1 , ( G ) Mmp2 , ( H ) Mmp9 , and ( I ) Pai-1 mRNA expression levels in liver tissue analyzed by qRT-PCR. (*: p < 0.05).
    Figure Legend Snippet: Effect of Fhl2 deficiency on hepatic fibrosis in the mouse model of bile duct ligation (BDL). Fhl2 -deficient ( Fhl2 -ko) and wild type (wt) mice were either subjected to BDL or were sham-operated (CTR). ( A ) Hepatic α-sma mRNA expression levels. ( B ) Immunohistochemical α-sma staining of liver tissue sections (20× magnification). Hepatic ( C ) Tgf-β and ( D ) Col1a1 mRNA expression levels. (E) Sirius Red/Fast Green staining of liver tissue sections (20× magnification). Hepatic ( F ) Mmp1 , ( G ) Mmp2 , ( H ) Mmp9 , and ( I ) Pai-1 mRNA expression levels in liver tissue analyzed by qRT-PCR. (*: p < 0.05).

    Techniques Used: Ligation, Expressing, Immunohistochemical staining, Staining, Quantitative RT-PCR

    Effect of FHL2 depletion on bile acid-induced hepatocellular injury in vitro. ( A , B ) FHL2 mRNA and protein expression in HepG2 cells transfected with si-pools against FHL2 (siFHL2) and si-control-pools (siCtr). ( C ) Representative microscopical images 72 h after transfection (10X magnification). ( D ) Quantification of lactate dehydrogenase (LDH) release into the supernatant. ( E ) FHL2 , ( F ) p47phox , and ( H ) BAX mRNA, and ( G ) B-cell lymphoma 2 (BCL2) protein expression after treatment with deoxycholic acid (DCA) for 24 h and control cells (CTR). (*: p < 0.05).
    Figure Legend Snippet: Effect of FHL2 depletion on bile acid-induced hepatocellular injury in vitro. ( A , B ) FHL2 mRNA and protein expression in HepG2 cells transfected with si-pools against FHL2 (siFHL2) and si-control-pools (siCtr). ( C ) Representative microscopical images 72 h after transfection (10X magnification). ( D ) Quantification of lactate dehydrogenase (LDH) release into the supernatant. ( E ) FHL2 , ( F ) p47phox , and ( H ) BAX mRNA, and ( G ) B-cell lymphoma 2 (BCL2) protein expression after treatment with deoxycholic acid (DCA) for 24 h and control cells (CTR). (*: p < 0.05).

    Techniques Used: In Vitro, Expressing, Transfection

    Effect of FHL2 depletion on bile acid metabolism. HepG2 cells were transfected with si-pools against FHL2 (siFHL2) and si-control-pools (siCtr). Analysis of ( A ) CYP7A1 , ( B ) NTCP , ( C ) BSEP , and ( D ) MRP2 mRNA expression after treatment with deoxycholic acid (DCA) for 24 h and control cells (CTR). (*: p < 0.05).
    Figure Legend Snippet: Effect of FHL2 depletion on bile acid metabolism. HepG2 cells were transfected with si-pools against FHL2 (siFHL2) and si-control-pools (siCtr). Analysis of ( A ) CYP7A1 , ( B ) NTCP , ( C ) BSEP , and ( D ) MRP2 mRNA expression after treatment with deoxycholic acid (DCA) for 24 h and control cells (CTR). (*: p < 0.05).

    Techniques Used: Transfection, Expressing

    Effect of FHL2 depletion on pro-fibrogenic gene expression in hepatic stellate cells in vitro. ( A ) FHL2 mRNA expression during activation of primary human hepatic stellate cells (HSCs). ( B , C ) FHL2 mRNA and protein expression in LX-2 cells transfected with si-pools against FHL2 (siFHL2) and si-control-pools (siCtr). ( D ) Representative microscopical images 72 h after transfection (10× magnification). ( E , F ) α-SMA mRNA and protein expression. ( G ) COL1A1 , ( H ) TGF-β , ( I ) MMP1 , ( J ) MMP3 , ( K ) MMP2 , ( L ) MMP9 and ( M ) MMP10 mRNA expression and ( N , O ) MMP13 and ( P , Q ) MMP14 mRNA and protein expression 72 h after transfection. (*: p < 0.05).
    Figure Legend Snippet: Effect of FHL2 depletion on pro-fibrogenic gene expression in hepatic stellate cells in vitro. ( A ) FHL2 mRNA expression during activation of primary human hepatic stellate cells (HSCs). ( B , C ) FHL2 mRNA and protein expression in LX-2 cells transfected with si-pools against FHL2 (siFHL2) and si-control-pools (siCtr). ( D ) Representative microscopical images 72 h after transfection (10× magnification). ( E , F ) α-SMA mRNA and protein expression. ( G ) COL1A1 , ( H ) TGF-β , ( I ) MMP1 , ( J ) MMP3 , ( K ) MMP2 , ( L ) MMP9 and ( M ) MMP10 mRNA expression and ( N , O ) MMP13 and ( P , Q ) MMP14 mRNA and protein expression 72 h after transfection. (*: p < 0.05).

    Techniques Used: Expressing, In Vitro, Activation Assay, Transfection



    Similar Products

    mouse monoclonal anti fhl2  (Hycult Biotech)
    90
    Hycult Biotech mouse monoclonal anti fhl2
    Primer sequences for quantitative real-time PCR.
    Mouse Monoclonal Anti Fhl2, supplied by Hycult Biotech, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mouse monoclonal anti fhl2/product/Hycult Biotech
    Average 90 stars, based on 1 article reviews
    mouse monoclonal anti fhl2 - by Bioz Stars, 2025-05
    90/100 stars
    		  Buy from Supplier

    Image Search Results


    Primer sequences for quantitative real-time PCR.

    Journal: Cells

    Article Title: Four-And-A-Half LIM-Domain Protein 2 (FHL2) Deficiency Aggravates Cholestatic Liver Injury

    doi: 10.3390/cells9010248

    Figure Lengend Snippet: Primer sequences for quantitative real-time PCR.

    Article Snippet: Protein extraction from liver tissues, protein extraction from cells, and analysis by Western blotting were performed as described in [ ] by applying mouse monoclonal anti-FHL2 (HycultBiotech, Uden, The Netherlands; HM2136, 1:300), rabbit monoclonal anti-alpha-smooth muscle actin (α-SMA) (Abcam, Cambridge, United Kingdom; ab32575, 1:1000), rabbit monoclonal anti-B-cell lymphoma 2 (BCL2) (Epitomics, Burlingame, CA, USA; #1017, 1:1000), rabbit polyclonal anti-metalloproteinase 13 (anti-MMP13) (Abcam, Cambridge, United Kingdom; ab39012, 1:1000), rabbit polyclonal anti-MMP14 (Chemicon, Burlington, MA, USA; AB815, 1:1000) and mouse monoclonal anti-actin (ACTB; Merck Millipore, Billerica, MA, USA; MAB1501, 1:10,000) antibodies.

    Techniques:

    Fhl2 expression and effect of Fhl2 deficiency on hepatocellular injury and inflammation in the mouse model of bile duct ligation (BDL). Fhl2 -deficient ( Fhl2 -ko) and wild type (wt) mice were either bile duct ligated (BDL) or sham-operated (CTR). ( A ) Fhl2 mRNA levels in wt BDL and CTR mice analyzed by qRT-PCR. ( B ) Representative hematoxylin and eosin stainings of liver tissue samples (20× magnification). ( C ) ALT (alanine aminotransferase) and ( D ) bilirubin serum levels. ( E ) Hmox-1 and ( F ) Mcp-1 mRNA expression levels in liver tissue analyzed by qRT-PCR. ( G ) Immunohistochemical CD3 staining of liver tissue samples (20× magnification). ( H ) Il-1 and ( I ) Tnf mRNA expression levels in liver tissue analyzed by qRT-PCR. (*: p < 0.05).

    Journal: Cells

    Article Title: Four-And-A-Half LIM-Domain Protein 2 (FHL2) Deficiency Aggravates Cholestatic Liver Injury

    doi: 10.3390/cells9010248

    Figure Lengend Snippet: Fhl2 expression and effect of Fhl2 deficiency on hepatocellular injury and inflammation in the mouse model of bile duct ligation (BDL). Fhl2 -deficient ( Fhl2 -ko) and wild type (wt) mice were either bile duct ligated (BDL) or sham-operated (CTR). ( A ) Fhl2 mRNA levels in wt BDL and CTR mice analyzed by qRT-PCR. ( B ) Representative hematoxylin and eosin stainings of liver tissue samples (20× magnification). ( C ) ALT (alanine aminotransferase) and ( D ) bilirubin serum levels. ( E ) Hmox-1 and ( F ) Mcp-1 mRNA expression levels in liver tissue analyzed by qRT-PCR. ( G ) Immunohistochemical CD3 staining of liver tissue samples (20× magnification). ( H ) Il-1 and ( I ) Tnf mRNA expression levels in liver tissue analyzed by qRT-PCR. (*: p < 0.05).

    Article Snippet: Protein extraction from liver tissues, protein extraction from cells, and analysis by Western blotting were performed as described in [ ] by applying mouse monoclonal anti-FHL2 (HycultBiotech, Uden, The Netherlands; HM2136, 1:300), rabbit monoclonal anti-alpha-smooth muscle actin (α-SMA) (Abcam, Cambridge, United Kingdom; ab32575, 1:1000), rabbit monoclonal anti-B-cell lymphoma 2 (BCL2) (Epitomics, Burlingame, CA, USA; #1017, 1:1000), rabbit polyclonal anti-metalloproteinase 13 (anti-MMP13) (Abcam, Cambridge, United Kingdom; ab39012, 1:1000), rabbit polyclonal anti-MMP14 (Chemicon, Burlington, MA, USA; AB815, 1:1000) and mouse monoclonal anti-actin (ACTB; Merck Millipore, Billerica, MA, USA; MAB1501, 1:10,000) antibodies.

    Techniques: Expressing, Ligation, Quantitative RT-PCR, Immunohistochemical staining, Staining

    Effect of Fhl2 deficiency on hepatic fibrosis in the mouse model of bile duct ligation (BDL). Fhl2 -deficient ( Fhl2 -ko) and wild type (wt) mice were either subjected to BDL or were sham-operated (CTR). ( A ) Hepatic α-sma mRNA expression levels. ( B ) Immunohistochemical α-sma staining of liver tissue sections (20× magnification). Hepatic ( C ) Tgf-β and ( D ) Col1a1 mRNA expression levels. (E) Sirius Red/Fast Green staining of liver tissue sections (20× magnification). Hepatic ( F ) Mmp1 , ( G ) Mmp2 , ( H ) Mmp9 , and ( I ) Pai-1 mRNA expression levels in liver tissue analyzed by qRT-PCR. (*: p < 0.05).

    Journal: Cells

    Article Title: Four-And-A-Half LIM-Domain Protein 2 (FHL2) Deficiency Aggravates Cholestatic Liver Injury

    doi: 10.3390/cells9010248

    Figure Lengend Snippet: Effect of Fhl2 deficiency on hepatic fibrosis in the mouse model of bile duct ligation (BDL). Fhl2 -deficient ( Fhl2 -ko) and wild type (wt) mice were either subjected to BDL or were sham-operated (CTR). ( A ) Hepatic α-sma mRNA expression levels. ( B ) Immunohistochemical α-sma staining of liver tissue sections (20× magnification). Hepatic ( C ) Tgf-β and ( D ) Col1a1 mRNA expression levels. (E) Sirius Red/Fast Green staining of liver tissue sections (20× magnification). Hepatic ( F ) Mmp1 , ( G ) Mmp2 , ( H ) Mmp9 , and ( I ) Pai-1 mRNA expression levels in liver tissue analyzed by qRT-PCR. (*: p < 0.05).

    Article Snippet: Protein extraction from liver tissues, protein extraction from cells, and analysis by Western blotting were performed as described in [ ] by applying mouse monoclonal anti-FHL2 (HycultBiotech, Uden, The Netherlands; HM2136, 1:300), rabbit monoclonal anti-alpha-smooth muscle actin (α-SMA) (Abcam, Cambridge, United Kingdom; ab32575, 1:1000), rabbit monoclonal anti-B-cell lymphoma 2 (BCL2) (Epitomics, Burlingame, CA, USA; #1017, 1:1000), rabbit polyclonal anti-metalloproteinase 13 (anti-MMP13) (Abcam, Cambridge, United Kingdom; ab39012, 1:1000), rabbit polyclonal anti-MMP14 (Chemicon, Burlington, MA, USA; AB815, 1:1000) and mouse monoclonal anti-actin (ACTB; Merck Millipore, Billerica, MA, USA; MAB1501, 1:10,000) antibodies.

    Techniques: Ligation, Expressing, Immunohistochemical staining, Staining, Quantitative RT-PCR

    Effect of FHL2 depletion on bile acid-induced hepatocellular injury in vitro. ( A , B ) FHL2 mRNA and protein expression in HepG2 cells transfected with si-pools against FHL2 (siFHL2) and si-control-pools (siCtr). ( C ) Representative microscopical images 72 h after transfection (10X magnification). ( D ) Quantification of lactate dehydrogenase (LDH) release into the supernatant. ( E ) FHL2 , ( F ) p47phox , and ( H ) BAX mRNA, and ( G ) B-cell lymphoma 2 (BCL2) protein expression after treatment with deoxycholic acid (DCA) for 24 h and control cells (CTR). (*: p < 0.05).

    Journal: Cells

    Article Title: Four-And-A-Half LIM-Domain Protein 2 (FHL2) Deficiency Aggravates Cholestatic Liver Injury

    doi: 10.3390/cells9010248

    Figure Lengend Snippet: Effect of FHL2 depletion on bile acid-induced hepatocellular injury in vitro. ( A , B ) FHL2 mRNA and protein expression in HepG2 cells transfected with si-pools against FHL2 (siFHL2) and si-control-pools (siCtr). ( C ) Representative microscopical images 72 h after transfection (10X magnification). ( D ) Quantification of lactate dehydrogenase (LDH) release into the supernatant. ( E ) FHL2 , ( F ) p47phox , and ( H ) BAX mRNA, and ( G ) B-cell lymphoma 2 (BCL2) protein expression after treatment with deoxycholic acid (DCA) for 24 h and control cells (CTR). (*: p < 0.05).

    Article Snippet: Protein extraction from liver tissues, protein extraction from cells, and analysis by Western blotting were performed as described in [ ] by applying mouse monoclonal anti-FHL2 (HycultBiotech, Uden, The Netherlands; HM2136, 1:300), rabbit monoclonal anti-alpha-smooth muscle actin (α-SMA) (Abcam, Cambridge, United Kingdom; ab32575, 1:1000), rabbit monoclonal anti-B-cell lymphoma 2 (BCL2) (Epitomics, Burlingame, CA, USA; #1017, 1:1000), rabbit polyclonal anti-metalloproteinase 13 (anti-MMP13) (Abcam, Cambridge, United Kingdom; ab39012, 1:1000), rabbit polyclonal anti-MMP14 (Chemicon, Burlington, MA, USA; AB815, 1:1000) and mouse monoclonal anti-actin (ACTB; Merck Millipore, Billerica, MA, USA; MAB1501, 1:10,000) antibodies.

    Techniques: In Vitro, Expressing, Transfection

    Effect of FHL2 depletion on bile acid metabolism. HepG2 cells were transfected with si-pools against FHL2 (siFHL2) and si-control-pools (siCtr). Analysis of ( A ) CYP7A1 , ( B ) NTCP , ( C ) BSEP , and ( D ) MRP2 mRNA expression after treatment with deoxycholic acid (DCA) for 24 h and control cells (CTR). (*: p < 0.05).

    Journal: Cells

    Article Title: Four-And-A-Half LIM-Domain Protein 2 (FHL2) Deficiency Aggravates Cholestatic Liver Injury

    doi: 10.3390/cells9010248

    Figure Lengend Snippet: Effect of FHL2 depletion on bile acid metabolism. HepG2 cells were transfected with si-pools against FHL2 (siFHL2) and si-control-pools (siCtr). Analysis of ( A ) CYP7A1 , ( B ) NTCP , ( C ) BSEP , and ( D ) MRP2 mRNA expression after treatment with deoxycholic acid (DCA) for 24 h and control cells (CTR). (*: p < 0.05).

    Article Snippet: Protein extraction from liver tissues, protein extraction from cells, and analysis by Western blotting were performed as described in [ ] by applying mouse monoclonal anti-FHL2 (HycultBiotech, Uden, The Netherlands; HM2136, 1:300), rabbit monoclonal anti-alpha-smooth muscle actin (α-SMA) (Abcam, Cambridge, United Kingdom; ab32575, 1:1000), rabbit monoclonal anti-B-cell lymphoma 2 (BCL2) (Epitomics, Burlingame, CA, USA; #1017, 1:1000), rabbit polyclonal anti-metalloproteinase 13 (anti-MMP13) (Abcam, Cambridge, United Kingdom; ab39012, 1:1000), rabbit polyclonal anti-MMP14 (Chemicon, Burlington, MA, USA; AB815, 1:1000) and mouse monoclonal anti-actin (ACTB; Merck Millipore, Billerica, MA, USA; MAB1501, 1:10,000) antibodies.

    Techniques: Transfection, Expressing

    Effect of FHL2 depletion on pro-fibrogenic gene expression in hepatic stellate cells in vitro. ( A ) FHL2 mRNA expression during activation of primary human hepatic stellate cells (HSCs). ( B , C ) FHL2 mRNA and protein expression in LX-2 cells transfected with si-pools against FHL2 (siFHL2) and si-control-pools (siCtr). ( D ) Representative microscopical images 72 h after transfection (10× magnification). ( E , F ) α-SMA mRNA and protein expression. ( G ) COL1A1 , ( H ) TGF-β , ( I ) MMP1 , ( J ) MMP3 , ( K ) MMP2 , ( L ) MMP9 and ( M ) MMP10 mRNA expression and ( N , O ) MMP13 and ( P , Q ) MMP14 mRNA and protein expression 72 h after transfection. (*: p < 0.05).

    Journal: Cells

    Article Title: Four-And-A-Half LIM-Domain Protein 2 (FHL2) Deficiency Aggravates Cholestatic Liver Injury

    doi: 10.3390/cells9010248

    Figure Lengend Snippet: Effect of FHL2 depletion on pro-fibrogenic gene expression in hepatic stellate cells in vitro. ( A ) FHL2 mRNA expression during activation of primary human hepatic stellate cells (HSCs). ( B , C ) FHL2 mRNA and protein expression in LX-2 cells transfected with si-pools against FHL2 (siFHL2) and si-control-pools (siCtr). ( D ) Representative microscopical images 72 h after transfection (10× magnification). ( E , F ) α-SMA mRNA and protein expression. ( G ) COL1A1 , ( H ) TGF-β , ( I ) MMP1 , ( J ) MMP3 , ( K ) MMP2 , ( L ) MMP9 and ( M ) MMP10 mRNA expression and ( N , O ) MMP13 and ( P , Q ) MMP14 mRNA and protein expression 72 h after transfection. (*: p < 0.05).

    Article Snippet: Protein extraction from liver tissues, protein extraction from cells, and analysis by Western blotting were performed as described in [ ] by applying mouse monoclonal anti-FHL2 (HycultBiotech, Uden, The Netherlands; HM2136, 1:300), rabbit monoclonal anti-alpha-smooth muscle actin (α-SMA) (Abcam, Cambridge, United Kingdom; ab32575, 1:1000), rabbit monoclonal anti-B-cell lymphoma 2 (BCL2) (Epitomics, Burlingame, CA, USA; #1017, 1:1000), rabbit polyclonal anti-metalloproteinase 13 (anti-MMP13) (Abcam, Cambridge, United Kingdom; ab39012, 1:1000), rabbit polyclonal anti-MMP14 (Chemicon, Burlington, MA, USA; AB815, 1:1000) and mouse monoclonal anti-actin (ACTB; Merck Millipore, Billerica, MA, USA; MAB1501, 1:10,000) antibodies.

    Techniques: Expressing, In Vitro, Activation Assay, Transfection