non blocking anti cd11b  (Hycult Biotech)


Bioz Verified Symbol Hycult Biotech is a verified supplier
Bioz Manufacturer Symbol Hycult Biotech manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 91

    Structured Review

    Hycult Biotech non blocking anti cd11b
    Mac-1 ligand surface induces EV production from adherent PMNs. (A) Comparison of EV production of adherent PMNs on BSA and surface-bound Mac-1 ligands. Dot scatter bars represent the EV quantification by flow cytometry, square scatter bars represent the quantification based on protein amount measurement. The EV production was measured on BSA surface (20 µg/ml), on C3bi surface (50 µg/ml) and on FH surface (50 µg/ml) for 20 minutes. N=3, error bars represent mean ± S.E.M. Data were compared to BSA by using RM one-way ANOVA coupled with Dunett’s post hoc test. (B) Comparison of the concentration of EV samples generated by adherent and soluble PMNs measured by NTA. C3bi (50 µg/ml) and BSA (20 µg/ml) were used in the previously detailed concentration. N=3, error bars represent mean ± S.E.M. Data were compared to BSA surface by using RM one-way ANOVA coupled with Dunett’s multiple comparisons test. (C) Comparison of EV production of PMNs induced by soluble Mac-1 ligands in different concentrations. The quantification of the EVs was carried out by flow cytometry. N=3, error bars represent mean ± S.E.M. Data were compared to spontaneous EV production (sEV) by using RM one-way ANOVA coupled with Dunett’s post hoc test. (D) Representative diagram of size distribution of PMN EVs induced by soluble or surface-bound C3bi measured by NTA. (E) Spontaneous and opsonized zymosan induced EV production of suspended PMNs in the presence of <t>anti-CD11b</t> (clone: ICRF44) and anti-CD11c (clone: 3.9) inhibitory antibodies. The quantification of the EVs was carried out by flow cytometry, EVs were labelled with FITC-Annexin V. N=3, error bars represent mean ± S.E.M. Data were compared by using RM one-way ANOVA coupled with Sidak’s multiple comparison test. # P < 0.05 and **P < 0.01; n.s., non significant.
    Non Blocking Anti Cd11b, supplied by Hycult Biotech, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/non blocking anti cd11b/product/Hycult Biotech
    Average 91 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    non blocking anti cd11b - by Bioz Stars, 2024-03
    91/100 stars

    Images

    1) Product Images from "Mac-1 Receptor Clustering Initiates Production of Pro-Inflammatory, Antibacterial Extracellular Vesicles From Neutrophils"

    Article Title: Mac-1 Receptor Clustering Initiates Production of Pro-Inflammatory, Antibacterial Extracellular Vesicles From Neutrophils

    Journal: Frontiers in Immunology

    doi: 10.3389/fimmu.2021.671995

    Mac-1 ligand surface induces EV production from adherent PMNs. (A) Comparison of EV production of adherent PMNs on BSA and surface-bound Mac-1 ligands. Dot scatter bars represent the EV quantification by flow cytometry, square scatter bars represent the quantification based on protein amount measurement. The EV production was measured on BSA surface (20 µg/ml), on C3bi surface (50 µg/ml) and on FH surface (50 µg/ml) for 20 minutes. N=3, error bars represent mean ± S.E.M. Data were compared to BSA by using RM one-way ANOVA coupled with Dunett’s post hoc test. (B) Comparison of the concentration of EV samples generated by adherent and soluble PMNs measured by NTA. C3bi (50 µg/ml) and BSA (20 µg/ml) were used in the previously detailed concentration. N=3, error bars represent mean ± S.E.M. Data were compared to BSA surface by using RM one-way ANOVA coupled with Dunett’s multiple comparisons test. (C) Comparison of EV production of PMNs induced by soluble Mac-1 ligands in different concentrations. The quantification of the EVs was carried out by flow cytometry. N=3, error bars represent mean ± S.E.M. Data were compared to spontaneous EV production (sEV) by using RM one-way ANOVA coupled with Dunett’s post hoc test. (D) Representative diagram of size distribution of PMN EVs induced by soluble or surface-bound C3bi measured by NTA. (E) Spontaneous and opsonized zymosan induced EV production of suspended PMNs in the presence of anti-CD11b (clone: ICRF44) and anti-CD11c (clone: 3.9) inhibitory antibodies. The quantification of the EVs was carried out by flow cytometry, EVs were labelled with FITC-Annexin V. N=3, error bars represent mean ± S.E.M. Data were compared by using RM one-way ANOVA coupled with Sidak’s multiple comparison test. # P < 0.05 and **P < 0.01; n.s., non significant.
    Figure Legend Snippet: Mac-1 ligand surface induces EV production from adherent PMNs. (A) Comparison of EV production of adherent PMNs on BSA and surface-bound Mac-1 ligands. Dot scatter bars represent the EV quantification by flow cytometry, square scatter bars represent the quantification based on protein amount measurement. The EV production was measured on BSA surface (20 µg/ml), on C3bi surface (50 µg/ml) and on FH surface (50 µg/ml) for 20 minutes. N=3, error bars represent mean ± S.E.M. Data were compared to BSA by using RM one-way ANOVA coupled with Dunett’s post hoc test. (B) Comparison of the concentration of EV samples generated by adherent and soluble PMNs measured by NTA. C3bi (50 µg/ml) and BSA (20 µg/ml) were used in the previously detailed concentration. N=3, error bars represent mean ± S.E.M. Data were compared to BSA surface by using RM one-way ANOVA coupled with Dunett’s multiple comparisons test. (C) Comparison of EV production of PMNs induced by soluble Mac-1 ligands in different concentrations. The quantification of the EVs was carried out by flow cytometry. N=3, error bars represent mean ± S.E.M. Data were compared to spontaneous EV production (sEV) by using RM one-way ANOVA coupled with Dunett’s post hoc test. (D) Representative diagram of size distribution of PMN EVs induced by soluble or surface-bound C3bi measured by NTA. (E) Spontaneous and opsonized zymosan induced EV production of suspended PMNs in the presence of anti-CD11b (clone: ICRF44) and anti-CD11c (clone: 3.9) inhibitory antibodies. The quantification of the EVs was carried out by flow cytometry, EVs were labelled with FITC-Annexin V. N=3, error bars represent mean ± S.E.M. Data were compared by using RM one-way ANOVA coupled with Sidak’s multiple comparison test. # P < 0.05 and **P < 0.01; n.s., non significant.

    Techniques Used: Flow Cytometry, Concentration Assay, Generated

    Mac-1 receptor clustering on BSA and C3bi surface. (A, B) Representative TIRF microscopic images of the cells after 20 minutes on coated surfaces. Cells were labelled with Alexa647-conjugated anti-CD11b. (A) BSA-coated surface, (B) C3bi-coated surface captured at the 20 th minute time point. (C) Median fluorescence intensity values of the ranked intensities of all the cells measured along a standard 25 μm line. Three independent experiments. Error bars represent mean ± S.E.M. Data were compared by linear regression. The two datasets are significantly different (p<0.0001). (D) Median of peak intensity values of the clusters in the samples. Three independent experiments, error bars represent mean ± S.E.M. Data were compared by using Students’s t-test. *P < 0.05.
    Figure Legend Snippet: Mac-1 receptor clustering on BSA and C3bi surface. (A, B) Representative TIRF microscopic images of the cells after 20 minutes on coated surfaces. Cells were labelled with Alexa647-conjugated anti-CD11b. (A) BSA-coated surface, (B) C3bi-coated surface captured at the 20 th minute time point. (C) Median fluorescence intensity values of the ranked intensities of all the cells measured along a standard 25 μm line. Three independent experiments. Error bars represent mean ± S.E.M. Data were compared by linear regression. The two datasets are significantly different (p<0.0001). (D) Median of peak intensity values of the clusters in the samples. Three independent experiments, error bars represent mean ± S.E.M. Data were compared by using Students’s t-test. *P < 0.05.

    Techniques Used: Fluorescence

    non blocking anti cd11b  (Hycult Biotech)


    Bioz Verified Symbol Hycult Biotech is a verified supplier
    Bioz Manufacturer Symbol Hycult Biotech manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 91

    Structured Review

    Hycult Biotech non blocking anti cd11b
    Mac-1 ligand surface induces EV production from adherent PMNs. (A) Comparison of EV production of adherent PMNs on BSA and surface-bound Mac-1 ligands. Dot scatter bars represent the EV quantification by flow cytometry, square scatter bars represent the quantification based on protein amount measurement. The EV production was measured on BSA surface (20 µg/ml), on C3bi surface (50 µg/ml) and on FH surface (50 µg/ml) for 20 minutes. N=3, error bars represent mean ± S.E.M. Data were compared to BSA by using RM one-way ANOVA coupled with Dunett’s post hoc test. (B) Comparison of the concentration of EV samples generated by adherent and soluble PMNs measured by NTA. C3bi (50 µg/ml) and BSA (20 µg/ml) were used in the previously detailed concentration. N=3, error bars represent mean ± S.E.M. Data were compared to BSA surface by using RM one-way ANOVA coupled with Dunett’s multiple comparisons test. (C) Comparison of EV production of PMNs induced by soluble Mac-1 ligands in different concentrations. The quantification of the EVs was carried out by flow cytometry. N=3, error bars represent mean ± S.E.M. Data were compared to spontaneous EV production (sEV) by using RM one-way ANOVA coupled with Dunett’s post hoc test. (D) Representative diagram of size distribution of PMN EVs induced by soluble or surface-bound C3bi measured by NTA. (E) Spontaneous and opsonized zymosan induced EV production of suspended PMNs in the presence of <t>anti-CD11b</t> (clone: ICRF44) and anti-CD11c (clone: 3.9) inhibitory antibodies. The quantification of the EVs was carried out by flow cytometry, EVs were labelled with FITC-Annexin V. N=3, error bars represent mean ± S.E.M. Data were compared by using RM one-way ANOVA coupled with Sidak’s multiple comparison test. # P < 0.05 and **P < 0.01; n.s., non significant.
    Non Blocking Anti Cd11b, supplied by Hycult Biotech, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/non blocking anti cd11b/product/Hycult Biotech
    Average 91 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    non blocking anti cd11b - by Bioz Stars, 2024-03
    91/100 stars

    Images

    1) Product Images from "Mac-1 Receptor Clustering Initiates Production of Pro-Inflammatory, Antibacterial Extracellular Vesicles From Neutrophils"

    Article Title: Mac-1 Receptor Clustering Initiates Production of Pro-Inflammatory, Antibacterial Extracellular Vesicles From Neutrophils

    Journal: Frontiers in Immunology

    doi: 10.3389/fimmu.2021.671995

    Mac-1 ligand surface induces EV production from adherent PMNs. (A) Comparison of EV production of adherent PMNs on BSA and surface-bound Mac-1 ligands. Dot scatter bars represent the EV quantification by flow cytometry, square scatter bars represent the quantification based on protein amount measurement. The EV production was measured on BSA surface (20 µg/ml), on C3bi surface (50 µg/ml) and on FH surface (50 µg/ml) for 20 minutes. N=3, error bars represent mean ± S.E.M. Data were compared to BSA by using RM one-way ANOVA coupled with Dunett’s post hoc test. (B) Comparison of the concentration of EV samples generated by adherent and soluble PMNs measured by NTA. C3bi (50 µg/ml) and BSA (20 µg/ml) were used in the previously detailed concentration. N=3, error bars represent mean ± S.E.M. Data were compared to BSA surface by using RM one-way ANOVA coupled with Dunett’s multiple comparisons test. (C) Comparison of EV production of PMNs induced by soluble Mac-1 ligands in different concentrations. The quantification of the EVs was carried out by flow cytometry. N=3, error bars represent mean ± S.E.M. Data were compared to spontaneous EV production (sEV) by using RM one-way ANOVA coupled with Dunett’s post hoc test. (D) Representative diagram of size distribution of PMN EVs induced by soluble or surface-bound C3bi measured by NTA. (E) Spontaneous and opsonized zymosan induced EV production of suspended PMNs in the presence of anti-CD11b (clone: ICRF44) and anti-CD11c (clone: 3.9) inhibitory antibodies. The quantification of the EVs was carried out by flow cytometry, EVs were labelled with FITC-Annexin V. N=3, error bars represent mean ± S.E.M. Data were compared by using RM one-way ANOVA coupled with Sidak’s multiple comparison test. # P < 0.05 and **P < 0.01; n.s., non significant.
    Figure Legend Snippet: Mac-1 ligand surface induces EV production from adherent PMNs. (A) Comparison of EV production of adherent PMNs on BSA and surface-bound Mac-1 ligands. Dot scatter bars represent the EV quantification by flow cytometry, square scatter bars represent the quantification based on protein amount measurement. The EV production was measured on BSA surface (20 µg/ml), on C3bi surface (50 µg/ml) and on FH surface (50 µg/ml) for 20 minutes. N=3, error bars represent mean ± S.E.M. Data were compared to BSA by using RM one-way ANOVA coupled with Dunett’s post hoc test. (B) Comparison of the concentration of EV samples generated by adherent and soluble PMNs measured by NTA. C3bi (50 µg/ml) and BSA (20 µg/ml) were used in the previously detailed concentration. N=3, error bars represent mean ± S.E.M. Data were compared to BSA surface by using RM one-way ANOVA coupled with Dunett’s multiple comparisons test. (C) Comparison of EV production of PMNs induced by soluble Mac-1 ligands in different concentrations. The quantification of the EVs was carried out by flow cytometry. N=3, error bars represent mean ± S.E.M. Data were compared to spontaneous EV production (sEV) by using RM one-way ANOVA coupled with Dunett’s post hoc test. (D) Representative diagram of size distribution of PMN EVs induced by soluble or surface-bound C3bi measured by NTA. (E) Spontaneous and opsonized zymosan induced EV production of suspended PMNs in the presence of anti-CD11b (clone: ICRF44) and anti-CD11c (clone: 3.9) inhibitory antibodies. The quantification of the EVs was carried out by flow cytometry, EVs were labelled with FITC-Annexin V. N=3, error bars represent mean ± S.E.M. Data were compared by using RM one-way ANOVA coupled with Sidak’s multiple comparison test. # P < 0.05 and **P < 0.01; n.s., non significant.

    Techniques Used: Flow Cytometry, Concentration Assay, Generated

    Mac-1 receptor clustering on BSA and C3bi surface. (A, B) Representative TIRF microscopic images of the cells after 20 minutes on coated surfaces. Cells were labelled with Alexa647-conjugated anti-CD11b. (A) BSA-coated surface, (B) C3bi-coated surface captured at the 20 th minute time point. (C) Median fluorescence intensity values of the ranked intensities of all the cells measured along a standard 25 μm line. Three independent experiments. Error bars represent mean ± S.E.M. Data were compared by linear regression. The two datasets are significantly different (p<0.0001). (D) Median of peak intensity values of the clusters in the samples. Three independent experiments, error bars represent mean ± S.E.M. Data were compared by using Students’s t-test. *P < 0.05.
    Figure Legend Snippet: Mac-1 receptor clustering on BSA and C3bi surface. (A, B) Representative TIRF microscopic images of the cells after 20 minutes on coated surfaces. Cells were labelled with Alexa647-conjugated anti-CD11b. (A) BSA-coated surface, (B) C3bi-coated surface captured at the 20 th minute time point. (C) Median fluorescence intensity values of the ranked intensities of all the cells measured along a standard 25 μm line. Three independent experiments. Error bars represent mean ± S.E.M. Data were compared by linear regression. The two datasets are significantly different (p<0.0001). (D) Median of peak intensity values of the clusters in the samples. Three independent experiments, error bars represent mean ± S.E.M. Data were compared by using Students’s t-test. *P < 0.05.

    Techniques Used: Fluorescence

    anti cd11b  (Hycult Biotech)


    Bioz Verified Symbol Hycult Biotech is a verified supplier
    Bioz Manufacturer Symbol Hycult Biotech manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 91

    Structured Review

    Hycult Biotech anti cd11b
    Anti Cd11b, supplied by Hycult Biotech, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti cd11b/product/Hycult Biotech
    Average 91 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    anti cd11b - by Bioz Stars, 2024-03
    91/100 stars

    Images

    Similar Products

  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 91
    Hycult Biotech non blocking anti cd11b
    Mac-1 ligand surface induces EV production from adherent PMNs. (A) Comparison of EV production of adherent PMNs on BSA and surface-bound Mac-1 ligands. Dot scatter bars represent the EV quantification by flow cytometry, square scatter bars represent the quantification based on protein amount measurement. The EV production was measured on BSA surface (20 µg/ml), on C3bi surface (50 µg/ml) and on FH surface (50 µg/ml) for 20 minutes. N=3, error bars represent mean ± S.E.M. Data were compared to BSA by using RM one-way ANOVA coupled with Dunett’s post hoc test. (B) Comparison of the concentration of EV samples generated by adherent and soluble PMNs measured by NTA. C3bi (50 µg/ml) and BSA (20 µg/ml) were used in the previously detailed concentration. N=3, error bars represent mean ± S.E.M. Data were compared to BSA surface by using RM one-way ANOVA coupled with Dunett’s multiple comparisons test. (C) Comparison of EV production of PMNs induced by soluble Mac-1 ligands in different concentrations. The quantification of the EVs was carried out by flow cytometry. N=3, error bars represent mean ± S.E.M. Data were compared to spontaneous EV production (sEV) by using RM one-way ANOVA coupled with Dunett’s post hoc test. (D) Representative diagram of size distribution of PMN EVs induced by soluble or surface-bound C3bi measured by NTA. (E) Spontaneous and opsonized zymosan induced EV production of suspended PMNs in the presence of <t>anti-CD11b</t> (clone: ICRF44) and anti-CD11c (clone: 3.9) inhibitory antibodies. The quantification of the EVs was carried out by flow cytometry, EVs were labelled with FITC-Annexin V. N=3, error bars represent mean ± S.E.M. Data were compared by using RM one-way ANOVA coupled with Sidak’s multiple comparison test. # P < 0.05 and **P < 0.01; n.s., non significant.
    Non Blocking Anti Cd11b, supplied by Hycult Biotech, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/non blocking anti cd11b/product/Hycult Biotech
    Average 91 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    non blocking anti cd11b - by Bioz Stars, 2024-03
    91/100 stars
      Buy from Supplier

    91
    Hycult Biotech anti cd11b
    Mac-1 ligand surface induces EV production from adherent PMNs. (A) Comparison of EV production of adherent PMNs on BSA and surface-bound Mac-1 ligands. Dot scatter bars represent the EV quantification by flow cytometry, square scatter bars represent the quantification based on protein amount measurement. The EV production was measured on BSA surface (20 µg/ml), on C3bi surface (50 µg/ml) and on FH surface (50 µg/ml) for 20 minutes. N=3, error bars represent mean ± S.E.M. Data were compared to BSA by using RM one-way ANOVA coupled with Dunett’s post hoc test. (B) Comparison of the concentration of EV samples generated by adherent and soluble PMNs measured by NTA. C3bi (50 µg/ml) and BSA (20 µg/ml) were used in the previously detailed concentration. N=3, error bars represent mean ± S.E.M. Data were compared to BSA surface by using RM one-way ANOVA coupled with Dunett’s multiple comparisons test. (C) Comparison of EV production of PMNs induced by soluble Mac-1 ligands in different concentrations. The quantification of the EVs was carried out by flow cytometry. N=3, error bars represent mean ± S.E.M. Data were compared to spontaneous EV production (sEV) by using RM one-way ANOVA coupled with Dunett’s post hoc test. (D) Representative diagram of size distribution of PMN EVs induced by soluble or surface-bound C3bi measured by NTA. (E) Spontaneous and opsonized zymosan induced EV production of suspended PMNs in the presence of <t>anti-CD11b</t> (clone: ICRF44) and anti-CD11c (clone: 3.9) inhibitory antibodies. The quantification of the EVs was carried out by flow cytometry, EVs were labelled with FITC-Annexin V. N=3, error bars represent mean ± S.E.M. Data were compared by using RM one-way ANOVA coupled with Sidak’s multiple comparison test. # P < 0.05 and **P < 0.01; n.s., non significant.
    Anti Cd11b, supplied by Hycult Biotech, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti cd11b/product/Hycult Biotech
    Average 91 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    anti cd11b - by Bioz Stars, 2024-03
    91/100 stars
      Buy from Supplier

    Image Search Results


    Mac-1 ligand surface induces EV production from adherent PMNs. (A) Comparison of EV production of adherent PMNs on BSA and surface-bound Mac-1 ligands. Dot scatter bars represent the EV quantification by flow cytometry, square scatter bars represent the quantification based on protein amount measurement. The EV production was measured on BSA surface (20 µg/ml), on C3bi surface (50 µg/ml) and on FH surface (50 µg/ml) for 20 minutes. N=3, error bars represent mean ± S.E.M. Data were compared to BSA by using RM one-way ANOVA coupled with Dunett’s post hoc test. (B) Comparison of the concentration of EV samples generated by adherent and soluble PMNs measured by NTA. C3bi (50 µg/ml) and BSA (20 µg/ml) were used in the previously detailed concentration. N=3, error bars represent mean ± S.E.M. Data were compared to BSA surface by using RM one-way ANOVA coupled with Dunett’s multiple comparisons test. (C) Comparison of EV production of PMNs induced by soluble Mac-1 ligands in different concentrations. The quantification of the EVs was carried out by flow cytometry. N=3, error bars represent mean ± S.E.M. Data were compared to spontaneous EV production (sEV) by using RM one-way ANOVA coupled with Dunett’s post hoc test. (D) Representative diagram of size distribution of PMN EVs induced by soluble or surface-bound C3bi measured by NTA. (E) Spontaneous and opsonized zymosan induced EV production of suspended PMNs in the presence of anti-CD11b (clone: ICRF44) and anti-CD11c (clone: 3.9) inhibitory antibodies. The quantification of the EVs was carried out by flow cytometry, EVs were labelled with FITC-Annexin V. N=3, error bars represent mean ± S.E.M. Data were compared by using RM one-way ANOVA coupled with Sidak’s multiple comparison test. # P < 0.05 and **P < 0.01; n.s., non significant.

    Journal: Frontiers in Immunology

    Article Title: Mac-1 Receptor Clustering Initiates Production of Pro-Inflammatory, Antibacterial Extracellular Vesicles From Neutrophils

    doi: 10.3389/fimmu.2021.671995

    Figure Lengend Snippet: Mac-1 ligand surface induces EV production from adherent PMNs. (A) Comparison of EV production of adherent PMNs on BSA and surface-bound Mac-1 ligands. Dot scatter bars represent the EV quantification by flow cytometry, square scatter bars represent the quantification based on protein amount measurement. The EV production was measured on BSA surface (20 µg/ml), on C3bi surface (50 µg/ml) and on FH surface (50 µg/ml) for 20 minutes. N=3, error bars represent mean ± S.E.M. Data were compared to BSA by using RM one-way ANOVA coupled with Dunett’s post hoc test. (B) Comparison of the concentration of EV samples generated by adherent and soluble PMNs measured by NTA. C3bi (50 µg/ml) and BSA (20 µg/ml) were used in the previously detailed concentration. N=3, error bars represent mean ± S.E.M. Data were compared to BSA surface by using RM one-way ANOVA coupled with Dunett’s multiple comparisons test. (C) Comparison of EV production of PMNs induced by soluble Mac-1 ligands in different concentrations. The quantification of the EVs was carried out by flow cytometry. N=3, error bars represent mean ± S.E.M. Data were compared to spontaneous EV production (sEV) by using RM one-way ANOVA coupled with Dunett’s post hoc test. (D) Representative diagram of size distribution of PMN EVs induced by soluble or surface-bound C3bi measured by NTA. (E) Spontaneous and opsonized zymosan induced EV production of suspended PMNs in the presence of anti-CD11b (clone: ICRF44) and anti-CD11c (clone: 3.9) inhibitory antibodies. The quantification of the EVs was carried out by flow cytometry, EVs were labelled with FITC-Annexin V. N=3, error bars represent mean ± S.E.M. Data were compared by using RM one-way ANOVA coupled with Sidak’s multiple comparison test. # P < 0.05 and **P < 0.01; n.s., non significant.

    Article Snippet: The non-blocking anti-CD11b (clone: Bear-1) for the artificial cluster induction was from HycultBiotech (Uden, The Netherlands).

    Techniques: Flow Cytometry, Concentration Assay, Generated

    Mac-1 receptor clustering on BSA and C3bi surface. (A, B) Representative TIRF microscopic images of the cells after 20 minutes on coated surfaces. Cells were labelled with Alexa647-conjugated anti-CD11b. (A) BSA-coated surface, (B) C3bi-coated surface captured at the 20 th minute time point. (C) Median fluorescence intensity values of the ranked intensities of all the cells measured along a standard 25 μm line. Three independent experiments. Error bars represent mean ± S.E.M. Data were compared by linear regression. The two datasets are significantly different (p<0.0001). (D) Median of peak intensity values of the clusters in the samples. Three independent experiments, error bars represent mean ± S.E.M. Data were compared by using Students’s t-test. *P < 0.05.

    Journal: Frontiers in Immunology

    Article Title: Mac-1 Receptor Clustering Initiates Production of Pro-Inflammatory, Antibacterial Extracellular Vesicles From Neutrophils

    doi: 10.3389/fimmu.2021.671995

    Figure Lengend Snippet: Mac-1 receptor clustering on BSA and C3bi surface. (A, B) Representative TIRF microscopic images of the cells after 20 minutes on coated surfaces. Cells were labelled with Alexa647-conjugated anti-CD11b. (A) BSA-coated surface, (B) C3bi-coated surface captured at the 20 th minute time point. (C) Median fluorescence intensity values of the ranked intensities of all the cells measured along a standard 25 μm line. Three independent experiments. Error bars represent mean ± S.E.M. Data were compared by linear regression. The two datasets are significantly different (p<0.0001). (D) Median of peak intensity values of the clusters in the samples. Three independent experiments, error bars represent mean ± S.E.M. Data were compared by using Students’s t-test. *P < 0.05.

    Article Snippet: The non-blocking anti-CD11b (clone: Bear-1) for the artificial cluster induction was from HycultBiotech (Uden, The Netherlands).

    Techniques: Fluorescence