Journal:
Article Title: Pharmacological targeting of C5a receptors during organ preservation improves kidney graft survival
doi: 10.1111/j.1365-2249.2008.03678.x
Figure Lengend Snippet: Complement 5a receptor (C5aR) expression in cadaveric donor (CAD) and living-related donor (LRD) and its correlation with cold ischaemia time, kidney function and tubular cell apoptosis. (a, b) C5aR expression in glomeruli, tubules and the interstitium of human allografts from LRD (a, upper panels) or CAD (b, lower panels). C5aR staining is depicted by the white arrowheads in each panel. (c) Quantitative evaluation of C5aR expression in grafts from CAD (n = 13) and LRD (n = 12). Groups were compared by analysis of variance to determine statistical differences between treatment groups (see Material and methods). (d) Correlation between cold ischaemia time (CIT) and peak serum creatinine (sCr) levels (r = 0·97, P < 0·001). (e–g) Correlations between C5aR staining intensity (number of C5aR positive tubules/field) and peak sCr levels (r = 0·96, P < 0·001) (e), duration of CIT (r = 0·82, P < 0·001) (f) and the frequency of apoptotic cells in the allograft (r = 0·78, P = 0·001) (g).
Article Snippet: Complement 5a receptor immunohistochemistry on human biopsy material Paraffin-embedded sections were deparaffinized and rehydrated through two changes of xylene and graded alcohols, fixed with fresh 4% formaldehyde in PBS for 30 min at 4°C, blocked with normal goat serum and incubated with anti-human C5aR mAb (clone W17/1; Hycult Biotechnology) for 1 h at room temperature.
Techniques: Expressing, Staining