Journal: Journal of Clinical Medicine

Article Title: Immunohistochemical Results of Soft Tissues Around a New Implant Healing-Abutment Surface: A Human Study

doi: 10.3390/jcm9041009

Figure Lengend Snippet: Expression pattern of inflammation and junctional markers. Statistically significant differences between different surface treatment were found ( p < 0.001).

Article Snippet: Briefly, samples were then incubated with anti-occludin antibody 5 µg/mL (Abcam Cambridge, UK) or human neutrophil defensins (alpha-defensins, HNP1–3) (Hycult Biotech, Uden, The Netherlands) for 1 h. Successively slides were incubated with goat anti polyvalent antibody for 10 min and subsequently with peroxidase for 10 min. After incubation samples were washed and treated by 3,3’-Diaminobenzidine DAB substrate.

Techniques: Expressing

Journal: Journal of Clinical Medicine

Article Title: Immunohistochemical Results of Soft Tissues Around a New Implant Healing-Abutment Surface: A Human Study

doi: 10.3390/jcm9041009

Figure Lengend Snippet: Immunostaining against HNP1–3 of the region of the gingiva adherent to the laser-treated ( A ), to the point of passage between the two surfaces ( B ) (dotted blue line represents an arbitrary hypothetical line) and to machined ( C ) surfaces, as indicated. Magnification 10X. ( D ) Percentage of HNP1–3 immunostaining positive area in the lamina propria and in the epithelial layers, as indicated. Values are expressed as mean ±SD of five determinations in randomly chosen sections for each specimen. * indicates values statistically different ( p < 0.001) between region of the gingiva adherent to the laser-treated- or to machined surfaces, as indicated.

Article Snippet: Briefly, samples were then incubated with anti-occludin antibody 5 µg/mL (Abcam Cambridge, UK) or human neutrophil defensins (alpha-defensins, HNP1–3) (Hycult Biotech, Uden, The Netherlands) for 1 h. Successively slides were incubated with goat anti polyvalent antibody for 10 min and subsequently with peroxidase for 10 min. After incubation samples were washed and treated by 3,3’-Diaminobenzidine DAB substrate.

Techniques: Immunostaining