Journal: International Journal of Molecular Sciences

Article Title: A Polymorphism of Bactericidal/Permeability-Increasing Protein Affects Its Neutralization Efficiency towards Lipopolysaccharide

doi: 10.3390/ijms23031324

Figure Lengend Snippet: Equal binding of BPI variants to LPS. ( a , b ) Binding of BPI to solid-phase LPS as detected with αBPI antibody clone 4H5 ( a ) or αBPI antibody 125 newly generated in mice ( b ). K D values are provided. ( c ) BPI 216E and BPI 216K were pre-incubated with increasing concentrations of liquid-phase LPS before adding the mixture to LPS-coated plates. IC 50 values are depicted. Absorbance measured for binding of BPI to the LPS-coated plate without pre-incubation with liquid-phase LPS was set as a reference to 100% ( c ). Data are shown as the means ( left ) or means ± SEM ( right ) of three biological replicates. Statistical testing was performed using the Student’s ratio paired t -test. Significance is indicated by the p value.

Article Snippet: After washing, bound BPI was detected by murine anti-human BPI monoclonal antibody 4H5 (Hycult Biotech, Uden, The Netherlands) or antibody 125 followed by detection via an HRP-conjugated rabbit anti-mouse IgG (Dianova, Hamburg, Germany).

Techniques: Binding Assay, Generated, Incubation