mouse anti fabp3  (Hycult Biotech)


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    Hycult Biotech mouse anti fabp3
    Effects of I/R injury on <t>FABP3,</t> FABP5 and FABP7 expression levels in the brain. ( A ) The locations of samples. Brains were cut into four slices (2-mm thick) from the front of the cortex. The second slices (designated as con and ips slices) of the brain, including the cortex and striatum, were used for Western blot analysis and PGE 2 -content analysis in the following experiments. ( B – E ) Mice were subjected to right tMCAO for 2 h. At 6, 12, 24 and 48 h after reperfusion, the second slice of the right brain (ipsilateral) and the left brain (contralateral) areas were collected for Western blot analysis of FABPs levels. ( B ) Representative images of Western blots. Quantitative analyses of FABP3 ( C ), FABP5 ( D ) and FABP7 ( E ) expression levels in contralateral (black) and ipsilateral (blue) areas of the brains. # p < 0.05, ## p < 0.01 vs. the sham group (contralateral); * p < 0.05, ** p < 0.01 vs. the sham group (ipsilateral) (n = 6 per group). Differences were statistically analyzed using one-way analysis of variance (ANOVA) followed by Dunnett’s test.
    Mouse Anti Fabp3, supplied by Hycult Biotech, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 91 stars, based on 1 article reviews
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    Images

    1) Product Images from "Fatty Acid-Binding Proteins Aggravate Cerebral Ischemia-Reperfusion Injury in Mice"

    Article Title: Fatty Acid-Binding Proteins Aggravate Cerebral Ischemia-Reperfusion Injury in Mice

    Journal: Biomedicines

    doi: 10.3390/biomedicines9050529

    Effects of I/R injury on FABP3, FABP5 and FABP7 expression levels in the brain. ( A ) The locations of samples. Brains were cut into four slices (2-mm thick) from the front of the cortex. The second slices (designated as con and ips slices) of the brain, including the cortex and striatum, were used for Western blot analysis and PGE 2 -content analysis in the following experiments. ( B – E ) Mice were subjected to right tMCAO for 2 h. At 6, 12, 24 and 48 h after reperfusion, the second slice of the right brain (ipsilateral) and the left brain (contralateral) areas were collected for Western blot analysis of FABPs levels. ( B ) Representative images of Western blots. Quantitative analyses of FABP3 ( C ), FABP5 ( D ) and FABP7 ( E ) expression levels in contralateral (black) and ipsilateral (blue) areas of the brains. # p < 0.05, ## p < 0.01 vs. the sham group (contralateral); * p < 0.05, ** p < 0.01 vs. the sham group (ipsilateral) (n = 6 per group). Differences were statistically analyzed using one-way analysis of variance (ANOVA) followed by Dunnett’s test.
    Figure Legend Snippet: Effects of I/R injury on FABP3, FABP5 and FABP7 expression levels in the brain. ( A ) The locations of samples. Brains were cut into four slices (2-mm thick) from the front of the cortex. The second slices (designated as con and ips slices) of the brain, including the cortex and striatum, were used for Western blot analysis and PGE 2 -content analysis in the following experiments. ( B – E ) Mice were subjected to right tMCAO for 2 h. At 6, 12, 24 and 48 h after reperfusion, the second slice of the right brain (ipsilateral) and the left brain (contralateral) areas were collected for Western blot analysis of FABPs levels. ( B ) Representative images of Western blots. Quantitative analyses of FABP3 ( C ), FABP5 ( D ) and FABP7 ( E ) expression levels in contralateral (black) and ipsilateral (blue) areas of the brains. # p < 0.05, ## p < 0.01 vs. the sham group (contralateral); * p < 0.05, ** p < 0.01 vs. the sham group (ipsilateral) (n = 6 per group). Differences were statistically analyzed using one-way analysis of variance (ANOVA) followed by Dunnett’s test.

    Techniques Used: Expressing, Western Blot

    Immunofluorescence of FABP3, FABP5 and FABP7 in the cortexes of sham and I/R mice. ( A ) Representative micro-graphs of immunofluorescence staining of the cortical penumbra region (shown in the black box area) at 12 h after reperfusion. ( B ) Double staining for FABP3 (green) and NeuN (a neuronal marker, red) expression in sham mice ( B1 ) and I/R mice ( B2 , ipsilateral). ( C , D ) Double staining for FABP5 (green) and NeuN (red; C ) or Olig2 (an oligodendrocyte marker, red; D ) in sham mice and I/R mice (ipsilateral). ( E , F ) Double staining for FABP7 (green) and GFAP (an as-trocyte marker, red; E ) or Olig2 (red; F ). Scale bar = 50 μm. The two small images on the left show immunofluorescence for FABPs and cell markers, whereas the larger image on the right is a merged image.
    Figure Legend Snippet: Immunofluorescence of FABP3, FABP5 and FABP7 in the cortexes of sham and I/R mice. ( A ) Representative micro-graphs of immunofluorescence staining of the cortical penumbra region (shown in the black box area) at 12 h after reperfusion. ( B ) Double staining for FABP3 (green) and NeuN (a neuronal marker, red) expression in sham mice ( B1 ) and I/R mice ( B2 , ipsilateral). ( C , D ) Double staining for FABP5 (green) and NeuN (red; C ) or Olig2 (an oligodendrocyte marker, red; D ) in sham mice and I/R mice (ipsilateral). ( E , F ) Double staining for FABP7 (green) and GFAP (an as-trocyte marker, red; E ) or Olig2 (red; F ). Scale bar = 50 μm. The two small images on the left show immunofluorescence for FABPs and cell markers, whereas the larger image on the right is a merged image.

    Techniques Used: Immunofluorescence, Staining, Double Staining, Marker, Expressing

    Effects of MF6 administration on FABP3, FABP5 and FABP7 expression levels in I/R mice. Mice were subjected to right tMCAO for 2 h and administered 3 mg/kg MF6 at 30 min after reperfusion. At 12 h after reperfusion, the second slice of the right (ipsilateral) and left (contralateral) brain areas, including the cortex and striatum, were used for Western blot analysis of FABP levels. ( A ) Representative images of Western blots. Quantitative analyses of FABP3 ( B ), FABP5 ( C ) and FABP7 ( D ) proteins expression levels in the contralateral (black) and ipsilateral (blue) regions of the brains. ** p < 0.01 vs. the sham-treated group, administered the vehicle (contralateral/ipsilateral); # p < 0.05, ## p < 0.01 vs. the I/R-treated group, administered the vehicle (contralateral/ipsilateral) (n = 6 per group). Differences were statistically analyzed using two-way analysis of variance (ANOVA) followed by Student–Newman–Keuls test.
    Figure Legend Snippet: Effects of MF6 administration on FABP3, FABP5 and FABP7 expression levels in I/R mice. Mice were subjected to right tMCAO for 2 h and administered 3 mg/kg MF6 at 30 min after reperfusion. At 12 h after reperfusion, the second slice of the right (ipsilateral) and left (contralateral) brain areas, including the cortex and striatum, were used for Western blot analysis of FABP levels. ( A ) Representative images of Western blots. Quantitative analyses of FABP3 ( B ), FABP5 ( C ) and FABP7 ( D ) proteins expression levels in the contralateral (black) and ipsilateral (blue) regions of the brains. ** p < 0.01 vs. the sham-treated group, administered the vehicle (contralateral/ipsilateral); # p < 0.05, ## p < 0.01 vs. the I/R-treated group, administered the vehicle (contralateral/ipsilateral) (n = 6 per group). Differences were statistically analyzed using two-way analysis of variance (ANOVA) followed by Student–Newman–Keuls test.

    Techniques Used: Expressing, Western Blot

    mouse anti fabp3  (Hycult Biotech)


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    Structured Review

    Hycult Biotech mouse anti fabp3
    Effects of I/R injury on <t>FABP3,</t> FABP5 and FABP7 expression levels in the brain. ( A ) The locations of samples. Brains were cut into four slices (2-mm thick) from the front of the cortex. The second slices (designated as con and ips slices) of the brain, including the cortex and striatum, were used for Western blot analysis and PGE 2 -content analysis in the following experiments. ( B – E ) Mice were subjected to right tMCAO for 2 h. At 6, 12, 24 and 48 h after reperfusion, the second slice of the right brain (ipsilateral) and the left brain (contralateral) areas were collected for Western blot analysis of FABPs levels. ( B ) Representative images of Western blots. Quantitative analyses of FABP3 ( C ), FABP5 ( D ) and FABP7 ( E ) expression levels in contralateral (black) and ipsilateral (blue) areas of the brains. # p < 0.05, ## p < 0.01 vs. the sham group (contralateral); * p < 0.05, ** p < 0.01 vs. the sham group (ipsilateral) (n = 6 per group). Differences were statistically analyzed using one-way analysis of variance (ANOVA) followed by Dunnett’s test.
    Mouse Anti Fabp3, supplied by Hycult Biotech, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 91 stars, based on 1 article reviews
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    Images

    1) Product Images from "Fatty Acid-Binding Proteins Aggravate Cerebral Ischemia-Reperfusion Injury in Mice"

    Article Title: Fatty Acid-Binding Proteins Aggravate Cerebral Ischemia-Reperfusion Injury in Mice

    Journal: Biomedicines

    doi: 10.3390/biomedicines9050529

    Effects of I/R injury on FABP3, FABP5 and FABP7 expression levels in the brain. ( A ) The locations of samples. Brains were cut into four slices (2-mm thick) from the front of the cortex. The second slices (designated as con and ips slices) of the brain, including the cortex and striatum, were used for Western blot analysis and PGE 2 -content analysis in the following experiments. ( B – E ) Mice were subjected to right tMCAO for 2 h. At 6, 12, 24 and 48 h after reperfusion, the second slice of the right brain (ipsilateral) and the left brain (contralateral) areas were collected for Western blot analysis of FABPs levels. ( B ) Representative images of Western blots. Quantitative analyses of FABP3 ( C ), FABP5 ( D ) and FABP7 ( E ) expression levels in contralateral (black) and ipsilateral (blue) areas of the brains. # p < 0.05, ## p < 0.01 vs. the sham group (contralateral); * p < 0.05, ** p < 0.01 vs. the sham group (ipsilateral) (n = 6 per group). Differences were statistically analyzed using one-way analysis of variance (ANOVA) followed by Dunnett’s test.
    Figure Legend Snippet: Effects of I/R injury on FABP3, FABP5 and FABP7 expression levels in the brain. ( A ) The locations of samples. Brains were cut into four slices (2-mm thick) from the front of the cortex. The second slices (designated as con and ips slices) of the brain, including the cortex and striatum, were used for Western blot analysis and PGE 2 -content analysis in the following experiments. ( B – E ) Mice were subjected to right tMCAO for 2 h. At 6, 12, 24 and 48 h after reperfusion, the second slice of the right brain (ipsilateral) and the left brain (contralateral) areas were collected for Western blot analysis of FABPs levels. ( B ) Representative images of Western blots. Quantitative analyses of FABP3 ( C ), FABP5 ( D ) and FABP7 ( E ) expression levels in contralateral (black) and ipsilateral (blue) areas of the brains. # p < 0.05, ## p < 0.01 vs. the sham group (contralateral); * p < 0.05, ** p < 0.01 vs. the sham group (ipsilateral) (n = 6 per group). Differences were statistically analyzed using one-way analysis of variance (ANOVA) followed by Dunnett’s test.

    Techniques Used: Expressing, Western Blot

    Immunofluorescence of FABP3, FABP5 and FABP7 in the cortexes of sham and I/R mice. ( A ) Representative micro-graphs of immunofluorescence staining of the cortical penumbra region (shown in the black box area) at 12 h after reperfusion. ( B ) Double staining for FABP3 (green) and NeuN (a neuronal marker, red) expression in sham mice ( B1 ) and I/R mice ( B2 , ipsilateral). ( C , D ) Double staining for FABP5 (green) and NeuN (red; C ) or Olig2 (an oligodendrocyte marker, red; D ) in sham mice and I/R mice (ipsilateral). ( E , F ) Double staining for FABP7 (green) and GFAP (an as-trocyte marker, red; E ) or Olig2 (red; F ). Scale bar = 50 μm. The two small images on the left show immunofluorescence for FABPs and cell markers, whereas the larger image on the right is a merged image.
    Figure Legend Snippet: Immunofluorescence of FABP3, FABP5 and FABP7 in the cortexes of sham and I/R mice. ( A ) Representative micro-graphs of immunofluorescence staining of the cortical penumbra region (shown in the black box area) at 12 h after reperfusion. ( B ) Double staining for FABP3 (green) and NeuN (a neuronal marker, red) expression in sham mice ( B1 ) and I/R mice ( B2 , ipsilateral). ( C , D ) Double staining for FABP5 (green) and NeuN (red; C ) or Olig2 (an oligodendrocyte marker, red; D ) in sham mice and I/R mice (ipsilateral). ( E , F ) Double staining for FABP7 (green) and GFAP (an as-trocyte marker, red; E ) or Olig2 (red; F ). Scale bar = 50 μm. The two small images on the left show immunofluorescence for FABPs and cell markers, whereas the larger image on the right is a merged image.

    Techniques Used: Immunofluorescence, Staining, Double Staining, Marker, Expressing

    Effects of MF6 administration on FABP3, FABP5 and FABP7 expression levels in I/R mice. Mice were subjected to right tMCAO for 2 h and administered 3 mg/kg MF6 at 30 min after reperfusion. At 12 h after reperfusion, the second slice of the right (ipsilateral) and left (contralateral) brain areas, including the cortex and striatum, were used for Western blot analysis of FABP levels. ( A ) Representative images of Western blots. Quantitative analyses of FABP3 ( B ), FABP5 ( C ) and FABP7 ( D ) proteins expression levels in the contralateral (black) and ipsilateral (blue) regions of the brains. ** p < 0.01 vs. the sham-treated group, administered the vehicle (contralateral/ipsilateral); # p < 0.05, ## p < 0.01 vs. the I/R-treated group, administered the vehicle (contralateral/ipsilateral) (n = 6 per group). Differences were statistically analyzed using two-way analysis of variance (ANOVA) followed by Student–Newman–Keuls test.
    Figure Legend Snippet: Effects of MF6 administration on FABP3, FABP5 and FABP7 expression levels in I/R mice. Mice were subjected to right tMCAO for 2 h and administered 3 mg/kg MF6 at 30 min after reperfusion. At 12 h after reperfusion, the second slice of the right (ipsilateral) and left (contralateral) brain areas, including the cortex and striatum, were used for Western blot analysis of FABP levels. ( A ) Representative images of Western blots. Quantitative analyses of FABP3 ( B ), FABP5 ( C ) and FABP7 ( D ) proteins expression levels in the contralateral (black) and ipsilateral (blue) regions of the brains. ** p < 0.01 vs. the sham-treated group, administered the vehicle (contralateral/ipsilateral); # p < 0.05, ## p < 0.01 vs. the I/R-treated group, administered the vehicle (contralateral/ipsilateral) (n = 6 per group). Differences were statistically analyzed using two-way analysis of variance (ANOVA) followed by Student–Newman–Keuls test.

    Techniques Used: Expressing, Western Blot

    mouse monoclonal antibodies against fabp3  (Hycult Biotech)


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    Hycult Biotech mouse monoclonal antibodies against fabp3
    <t>FABP3</t> was strongly expressed in PV+ GABAergic interneurons of the ACC. A, Left, RT-PCR analysis of Fabp3 in different brain areas. Right, qPCR analysis of Fabp3 in different brain areas. Error bar indicates mean ± SEM. n = 6 mice per group. **p < 0.01 versus WT ACC. B–F, Confocal images showing colocalization of FABP3 and a pyramidal neuronal marker (Ng), three classical GABAergic neuronal makers (PV, SOM, and CR), or GAD67 in the ACC. B, Most FABP3+ structures do not show immunoreactivity for Ng. C, Confocal images showing FABP3 and PV colocalization in the ACC. D, F, Most FABP+ structures do not show immunoreactivity for SOM (D) or CR (E). F, Confocal images showing FABP3 and GAD67 colocalization in the ACC (arrowheads). B, C (bottom), D, E (bottom right), F (right), Enlarged images of the boxed area in the merged image. Scale bars, 50 μm. Str, Striatum.
    Mouse Monoclonal Antibodies Against Fabp3, supplied by Hycult Biotech, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mouse monoclonal antibodies against fabp3/product/Hycult Biotech
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    Images

    1) Product Images from "FABP3 in the Anterior Cingulate Cortex Modulates the Methylation Status of the Glutamic Acid Decarboxylase 67 Promoter Region"

    Article Title: FABP3 in the Anterior Cingulate Cortex Modulates the Methylation Status of the Glutamic Acid Decarboxylase 67 Promoter Region

    Journal: The Journal of Neuroscience

    doi: 10.1523/JNEUROSCI.1285-18.2018

    FABP3 was strongly expressed in PV+ GABAergic interneurons of the ACC. A, Left, RT-PCR analysis of Fabp3 in different brain areas. Right, qPCR analysis of Fabp3 in different brain areas. Error bar indicates mean ± SEM. n = 6 mice per group. **p < 0.01 versus WT ACC. B–F, Confocal images showing colocalization of FABP3 and a pyramidal neuronal marker (Ng), three classical GABAergic neuronal makers (PV, SOM, and CR), or GAD67 in the ACC. B, Most FABP3+ structures do not show immunoreactivity for Ng. C, Confocal images showing FABP3 and PV colocalization in the ACC. D, F, Most FABP+ structures do not show immunoreactivity for SOM (D) or CR (E). F, Confocal images showing FABP3 and GAD67 colocalization in the ACC (arrowheads). B, C (bottom), D, E (bottom right), F (right), Enlarged images of the boxed area in the merged image. Scale bars, 50 μm. Str, Striatum.
    Figure Legend Snippet: FABP3 was strongly expressed in PV+ GABAergic interneurons of the ACC. A, Left, RT-PCR analysis of Fabp3 in different brain areas. Right, qPCR analysis of Fabp3 in different brain areas. Error bar indicates mean ± SEM. n = 6 mice per group. **p < 0.01 versus WT ACC. B–F, Confocal images showing colocalization of FABP3 and a pyramidal neuronal marker (Ng), three classical GABAergic neuronal makers (PV, SOM, and CR), or GAD67 in the ACC. B, Most FABP3+ structures do not show immunoreactivity for Ng. C, Confocal images showing FABP3 and PV colocalization in the ACC. D, F, Most FABP+ structures do not show immunoreactivity for SOM (D) or CR (E). F, Confocal images showing FABP3 and GAD67 colocalization in the ACC (arrowheads). B, C (bottom), D, E (bottom right), F (right), Enlarged images of the boxed area in the merged image. Scale bars, 50 μm. Str, Striatum.

    Techniques Used: Reverse Transcription Polymerase Chain Reaction, Marker

    Colocalization of PV, SOM, and CR with  FABP3  in the ACC a
    Figure Legend Snippet: Colocalization of PV, SOM, and CR with FABP3 in the ACC a

    Techniques Used:

    Fabp3 gene ablation in the ACC caused upregulation of GAD67 expression and GABA synthesis. A, Quantitative analysis of the number of PV−, SOM−, and CR+ neurons in the ACC. PV, n = 8 sections, 4 mice; SOM, n = 12 sections, 6 mice; CR, n = 12 sections, 6 mice per group. B, Representative immunoblots (left) and quantitative densitometry analysis (right) probed with GABA-related antibodies. GAD67 and β-actin, n = 6 mice per group; GAD65, VGAT, and GABAAR, n = 5 mice per group; gephyrin, n = 5 and 6 WT and Fabp3 KO mice, respectively. C, Quantitative analysis of Gad67 mRNA expression in the ACC from WT and Fabp3 KO mice. n = 4 mice per group. D, Quantitative analysis of the GABA concentrations in ACC extracts from WT and Fabp3 KO mice by ELISA. n = 6 mice per group. E–G, There were no differences in GAD67 expression or GABA synthesis in the mPFC or AM of Fabp3 KO mice. E, Representative immunoblots (left) and quantitative densitometry analysis (right) probed with GAD67 antibody. n = 4 mice per group. F, Quantitative analysis of the Gad67 mRNA expression. n = 4 mice per group. G, Quantitative analysis of the GABA concentrations in the mPFC and AM extracts by ELISA. mPFC, n = 6 mice per group; AM, n = 4 and 6 WT and Fabp3 KO mice, respectively. H, I, Quantitative analysis of the DA and 5-HT concentrations in ACC extracts by ELISA. 5-HT, n = 6 mice per group; DA, n = 6 and 5 WT and Fabp3 KO mice, respectively. A–I, **p < 0.01 versus WT mice. J, Left, Representative immunoblots probed with FABP3 antibody. Right, Quantitative analysis of Gad67 mRNA in Neuro-2a cells. n = 4 per group. **p < 0.01 versus control. mock, Mock control; OE, Fabp3 overexpression; NC, negative control; KD, Fabp3 knockdown. Error bar indicates mean ± SEM.
    Figure Legend Snippet: Fabp3 gene ablation in the ACC caused upregulation of GAD67 expression and GABA synthesis. A, Quantitative analysis of the number of PV−, SOM−, and CR+ neurons in the ACC. PV, n = 8 sections, 4 mice; SOM, n = 12 sections, 6 mice; CR, n = 12 sections, 6 mice per group. B, Representative immunoblots (left) and quantitative densitometry analysis (right) probed with GABA-related antibodies. GAD67 and β-actin, n = 6 mice per group; GAD65, VGAT, and GABAAR, n = 5 mice per group; gephyrin, n = 5 and 6 WT and Fabp3 KO mice, respectively. C, Quantitative analysis of Gad67 mRNA expression in the ACC from WT and Fabp3 KO mice. n = 4 mice per group. D, Quantitative analysis of the GABA concentrations in ACC extracts from WT and Fabp3 KO mice by ELISA. n = 6 mice per group. E–G, There were no differences in GAD67 expression or GABA synthesis in the mPFC or AM of Fabp3 KO mice. E, Representative immunoblots (left) and quantitative densitometry analysis (right) probed with GAD67 antibody. n = 4 mice per group. F, Quantitative analysis of the Gad67 mRNA expression. n = 4 mice per group. G, Quantitative analysis of the GABA concentrations in the mPFC and AM extracts by ELISA. mPFC, n = 6 mice per group; AM, n = 4 and 6 WT and Fabp3 KO mice, respectively. H, I, Quantitative analysis of the DA and 5-HT concentrations in ACC extracts by ELISA. 5-HT, n = 6 mice per group; DA, n = 6 and 5 WT and Fabp3 KO mice, respectively. A–I, **p < 0.01 versus WT mice. J, Left, Representative immunoblots probed with FABP3 antibody. Right, Quantitative analysis of Gad67 mRNA in Neuro-2a cells. n = 4 per group. **p < 0.01 versus control. mock, Mock control; OE, Fabp3 overexpression; NC, negative control; KD, Fabp3 knockdown. Error bar indicates mean ± SEM.

    Techniques Used: Expressing, Western Blot, Enzyme-linked Immunosorbent Assay, Over Expression, Negative Control

    Enhancement of inhibitory synaptic plasticity in the ACC of Fabp3 KO mice. A, Representative traces of mEPSCs and mIPSCs in ACC layer II/III pyramidal neurons. B, Plots of the mEPSC and mIPSC frequencies in WT and Fabp3 KO mice. C, Plots of the mEPSC and mIPSC amplitudes in WT and Fabp3 KO mice. n = 53 cells for WT and 59 cells for Fabp3 KO from 5 mice for mEPSCs. n = 52 cells for WT and 60 cells for Fabp3 KO from 5 mice for mIPSCs. **p < 0.01 versus WT mice. Error bar indicates mean ± SEM.
    Figure Legend Snippet: Enhancement of inhibitory synaptic plasticity in the ACC of Fabp3 KO mice. A, Representative traces of mEPSCs and mIPSCs in ACC layer II/III pyramidal neurons. B, Plots of the mEPSC and mIPSC frequencies in WT and Fabp3 KO mice. C, Plots of the mEPSC and mIPSC amplitudes in WT and Fabp3 KO mice. n = 53 cells for WT and 59 cells for Fabp3 KO from 5 mice for mEPSCs. n = 52 cells for WT and 60 cells for Fabp3 KO from 5 mice for mIPSCs. **p < 0.01 versus WT mice. Error bar indicates mean ± SEM.

    Techniques Used:

    Extracellular glutamate (Glu) concentrations in the ACC in freely moving animals. A, Dialysate Glu concentrations at baseline (basal) and after depolarization stimulation (High-K+) (left). Glu AUC before (basal) and after (High-K+) depolarization stimulation (right). *p < 0.05 versus wild-type mice. B, Normalization of the dialysate Glu signals indicated that the response to depolarization stimulation was enhanced in Fabp3 KO mice (left). The normalized Glu AUC after depolarization stimulation (right). A, B, Error bar indicates mean ± SEM. *p < 0.05 versus WT mice. n = 6 WT mice; n = 5 Fabp3 KO mice. C, D, Representative immunoblots (left) and quantitative densitometry analysis (right) probed with various antibodies. *p < 0.05; **p < 0.01 versus WT mice. n = 6 mice per group. Error bar indicates mean ± SEM.
    Figure Legend Snippet: Extracellular glutamate (Glu) concentrations in the ACC in freely moving animals. A, Dialysate Glu concentrations at baseline (basal) and after depolarization stimulation (High-K+) (left). Glu AUC before (basal) and after (High-K+) depolarization stimulation (right). *p < 0.05 versus wild-type mice. B, Normalization of the dialysate Glu signals indicated that the response to depolarization stimulation was enhanced in Fabp3 KO mice (left). The normalized Glu AUC after depolarization stimulation (right). A, B, Error bar indicates mean ± SEM. *p < 0.05 versus WT mice. n = 6 WT mice; n = 5 Fabp3 KO mice. C, D, Representative immunoblots (left) and quantitative densitometry analysis (right) probed with various antibodies. *p < 0.05; **p < 0.01 versus WT mice. n = 6 mice per group. Error bar indicates mean ± SEM.

    Techniques Used: Western Blot

    Decreased binding of MeCP2 and HDAC1 to the Gad67 promoter region in the ACC of Fabp3 KO mice. A, Representative immunoblots (top) and quantitative densitometry analysis (bottom) probed with various antibodies are shown. n = 6 mice per group. B, ChIP assay of ACC extracts from WT or Fabp3 KO mice via anti-MeCP2 and HDAC1. The precipitated DNA was analyzed by qPCR with primers amplifying the Gad67 promoter region. **p < 0.01 versus WT mice. n = 5 WT mice. n = 4 Fabp3 KO mice. C, DNA methylation profile in the Gad67 promoter region determined by bisulfite sequencing. Black circles represent methylated CpGs. Open circles represent unmethylated sites. Top numbers indicate global percentages of methylated cytosines. D, Quantitative analysis of the Gad67 mRNA expression in the ACC. WT, n = 6; KO, n = 5; KO + MET, n = 6 mice per group. **p < 0.01 versus WT mice. ##p < 0.01 versus KO mice. E, F, Quantitative analysis of the SAM concentration by ELISA. n = 4 per group. **p < 0.01 versus mock control. MET treatment (5.2 mmol/kg, s.c., twice per day for 6 d). mock, Mock control; OE, Fabp3 overexpression. Error bar indicates mean ± SEM.
    Figure Legend Snippet: Decreased binding of MeCP2 and HDAC1 to the Gad67 promoter region in the ACC of Fabp3 KO mice. A, Representative immunoblots (top) and quantitative densitometry analysis (bottom) probed with various antibodies are shown. n = 6 mice per group. B, ChIP assay of ACC extracts from WT or Fabp3 KO mice via anti-MeCP2 and HDAC1. The precipitated DNA was analyzed by qPCR with primers amplifying the Gad67 promoter region. **p < 0.01 versus WT mice. n = 5 WT mice. n = 4 Fabp3 KO mice. C, DNA methylation profile in the Gad67 promoter region determined by bisulfite sequencing. Black circles represent methylated CpGs. Open circles represent unmethylated sites. Top numbers indicate global percentages of methylated cytosines. D, Quantitative analysis of the Gad67 mRNA expression in the ACC. WT, n = 6; KO, n = 5; KO + MET, n = 6 mice per group. **p < 0.01 versus WT mice. ##p < 0.01 versus KO mice. E, F, Quantitative analysis of the SAM concentration by ELISA. n = 4 per group. **p < 0.01 versus mock control. MET treatment (5.2 mmol/kg, s.c., twice per day for 6 d). mock, Mock control; OE, Fabp3 overexpression. Error bar indicates mean ± SEM.

    Techniques Used: Binding Assay, Western Blot, DNA Methylation Assay, Methylation Sequencing, Methylation, Expressing, Concentration Assay, Enzyme-linked Immunosorbent Assay, Over Expression

    Amelioration of behavioral abnormalities in Fabp3 KO mice by chronic treatment with MET. A, Schematic of the behavioral tests and MET treatment (5.2 mmol/kg, s.c., twice per day). During the behavioral testing period, mice received saline or MET once per day after testing. B, OFT. Graph represents the time spent in the center area over a 5 min period for WT and Fabp3 KO mice. n = 14–17 mice per group. C, HBT. An illustrative example of the travel pathway in the HBT using video tracking software (top). Graphs represent the time spent in the center area, total number of head-dips, head-dip duration, and latency to first head-dip for the 5 min testing session (bottom). n = 13–15 mice per group. **p < 0.01 versus saline-treated WT mice. ##p < 0.01 versus saline-treated KO mice. D, NORT. Differences in exploratory preference were assessed between groups in the training (left) or test (right) sessions. n = 11–15 mice per group. **p < 0.01 versus familiar group. Sal, Saline treatment. MET treatment (5.2 mmol/kg, s.c., twice per day for 6 d). Error bar indicates mean ± SEM.
    Figure Legend Snippet: Amelioration of behavioral abnormalities in Fabp3 KO mice by chronic treatment with MET. A, Schematic of the behavioral tests and MET treatment (5.2 mmol/kg, s.c., twice per day). During the behavioral testing period, mice received saline or MET once per day after testing. B, OFT. Graph represents the time spent in the center area over a 5 min period for WT and Fabp3 KO mice. n = 14–17 mice per group. C, HBT. An illustrative example of the travel pathway in the HBT using video tracking software (top). Graphs represent the time spent in the center area, total number of head-dips, head-dip duration, and latency to first head-dip for the 5 min testing session (bottom). n = 13–15 mice per group. **p < 0.01 versus saline-treated WT mice. ##p < 0.01 versus saline-treated KO mice. D, NORT. Differences in exploratory preference were assessed between groups in the training (left) or test (right) sessions. n = 11–15 mice per group. **p < 0.01 versus familiar group. Sal, Saline treatment. MET treatment (5.2 mmol/kg, s.c., twice per day for 6 d). Error bar indicates mean ± SEM.

    Techniques Used: Software

    Locomotor activities during behavioral tests a
    Figure Legend Snippet: Locomotor activities during behavioral tests a

    Techniques Used:

    mouse monoclonal antibodies against fabp3  (Hycult Biotech)


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    Structured Review

    Hycult Biotech mouse monoclonal antibodies against fabp3
    <t>FABP3</t> was strongly expressed in PV+ GABAergic interneurons of the ACC. A, Left, RT-PCR analysis of Fabp3 in different brain areas. Right, qPCR analysis of Fabp3 in different brain areas. Error bar indicates mean ± SEM. n = 6 mice per group. **p < 0.01 versus WT ACC. B–F, Confocal images showing colocalization of FABP3 and a pyramidal neuronal marker (Ng), three classical GABAergic neuronal makers (PV, SOM, and CR), or GAD67 in the ACC. B, Most FABP3+ structures do not show immunoreactivity for Ng. C, Confocal images showing FABP3 and PV colocalization in the ACC. D, F, Most FABP+ structures do not show immunoreactivity for SOM (D) or CR (E). F, Confocal images showing FABP3 and GAD67 colocalization in the ACC (arrowheads). B, C (bottom), D, E (bottom right), F (right), Enlarged images of the boxed area in the merged image. Scale bars, 50 μm. Str, Striatum.
    Mouse Monoclonal Antibodies Against Fabp3, supplied by Hycult Biotech, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mouse monoclonal antibodies against fabp3/product/Hycult Biotech
    Average 91 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    mouse monoclonal antibodies against fabp3 - by Bioz Stars, 2024-04
    91/100 stars

    Images

    1) Product Images from "FABP3 in the Anterior Cingulate Cortex Modulates the Methylation Status of the Glutamic Acid Decarboxylase 67 Promoter Region"

    Article Title: FABP3 in the Anterior Cingulate Cortex Modulates the Methylation Status of the Glutamic Acid Decarboxylase 67 Promoter Region

    Journal: The Journal of Neuroscience

    doi: 10.1523/JNEUROSCI.1285-18.2018

    FABP3 was strongly expressed in PV+ GABAergic interneurons of the ACC. A, Left, RT-PCR analysis of Fabp3 in different brain areas. Right, qPCR analysis of Fabp3 in different brain areas. Error bar indicates mean ± SEM. n = 6 mice per group. **p < 0.01 versus WT ACC. B–F, Confocal images showing colocalization of FABP3 and a pyramidal neuronal marker (Ng), three classical GABAergic neuronal makers (PV, SOM, and CR), or GAD67 in the ACC. B, Most FABP3+ structures do not show immunoreactivity for Ng. C, Confocal images showing FABP3 and PV colocalization in the ACC. D, F, Most FABP+ structures do not show immunoreactivity for SOM (D) or CR (E). F, Confocal images showing FABP3 and GAD67 colocalization in the ACC (arrowheads). B, C (bottom), D, E (bottom right), F (right), Enlarged images of the boxed area in the merged image. Scale bars, 50 μm. Str, Striatum.
    Figure Legend Snippet: FABP3 was strongly expressed in PV+ GABAergic interneurons of the ACC. A, Left, RT-PCR analysis of Fabp3 in different brain areas. Right, qPCR analysis of Fabp3 in different brain areas. Error bar indicates mean ± SEM. n = 6 mice per group. **p < 0.01 versus WT ACC. B–F, Confocal images showing colocalization of FABP3 and a pyramidal neuronal marker (Ng), three classical GABAergic neuronal makers (PV, SOM, and CR), or GAD67 in the ACC. B, Most FABP3+ structures do not show immunoreactivity for Ng. C, Confocal images showing FABP3 and PV colocalization in the ACC. D, F, Most FABP+ structures do not show immunoreactivity for SOM (D) or CR (E). F, Confocal images showing FABP3 and GAD67 colocalization in the ACC (arrowheads). B, C (bottom), D, E (bottom right), F (right), Enlarged images of the boxed area in the merged image. Scale bars, 50 μm. Str, Striatum.

    Techniques Used: Reverse Transcription Polymerase Chain Reaction, Marker

    Colocalization of PV, SOM, and CR with  FABP3  in the ACC a
    Figure Legend Snippet: Colocalization of PV, SOM, and CR with FABP3 in the ACC a

    Techniques Used:

    Fabp3 gene ablation in the ACC caused upregulation of GAD67 expression and GABA synthesis. A, Quantitative analysis of the number of PV−, SOM−, and CR+ neurons in the ACC. PV, n = 8 sections, 4 mice; SOM, n = 12 sections, 6 mice; CR, n = 12 sections, 6 mice per group. B, Representative immunoblots (left) and quantitative densitometry analysis (right) probed with GABA-related antibodies. GAD67 and β-actin, n = 6 mice per group; GAD65, VGAT, and GABAAR, n = 5 mice per group; gephyrin, n = 5 and 6 WT and Fabp3 KO mice, respectively. C, Quantitative analysis of Gad67 mRNA expression in the ACC from WT and Fabp3 KO mice. n = 4 mice per group. D, Quantitative analysis of the GABA concentrations in ACC extracts from WT and Fabp3 KO mice by ELISA. n = 6 mice per group. E–G, There were no differences in GAD67 expression or GABA synthesis in the mPFC or AM of Fabp3 KO mice. E, Representative immunoblots (left) and quantitative densitometry analysis (right) probed with GAD67 antibody. n = 4 mice per group. F, Quantitative analysis of the Gad67 mRNA expression. n = 4 mice per group. G, Quantitative analysis of the GABA concentrations in the mPFC and AM extracts by ELISA. mPFC, n = 6 mice per group; AM, n = 4 and 6 WT and Fabp3 KO mice, respectively. H, I, Quantitative analysis of the DA and 5-HT concentrations in ACC extracts by ELISA. 5-HT, n = 6 mice per group; DA, n = 6 and 5 WT and Fabp3 KO mice, respectively. A–I, **p < 0.01 versus WT mice. J, Left, Representative immunoblots probed with FABP3 antibody. Right, Quantitative analysis of Gad67 mRNA in Neuro-2a cells. n = 4 per group. **p < 0.01 versus control. mock, Mock control; OE, Fabp3 overexpression; NC, negative control; KD, Fabp3 knockdown. Error bar indicates mean ± SEM.
    Figure Legend Snippet: Fabp3 gene ablation in the ACC caused upregulation of GAD67 expression and GABA synthesis. A, Quantitative analysis of the number of PV−, SOM−, and CR+ neurons in the ACC. PV, n = 8 sections, 4 mice; SOM, n = 12 sections, 6 mice; CR, n = 12 sections, 6 mice per group. B, Representative immunoblots (left) and quantitative densitometry analysis (right) probed with GABA-related antibodies. GAD67 and β-actin, n = 6 mice per group; GAD65, VGAT, and GABAAR, n = 5 mice per group; gephyrin, n = 5 and 6 WT and Fabp3 KO mice, respectively. C, Quantitative analysis of Gad67 mRNA expression in the ACC from WT and Fabp3 KO mice. n = 4 mice per group. D, Quantitative analysis of the GABA concentrations in ACC extracts from WT and Fabp3 KO mice by ELISA. n = 6 mice per group. E–G, There were no differences in GAD67 expression or GABA synthesis in the mPFC or AM of Fabp3 KO mice. E, Representative immunoblots (left) and quantitative densitometry analysis (right) probed with GAD67 antibody. n = 4 mice per group. F, Quantitative analysis of the Gad67 mRNA expression. n = 4 mice per group. G, Quantitative analysis of the GABA concentrations in the mPFC and AM extracts by ELISA. mPFC, n = 6 mice per group; AM, n = 4 and 6 WT and Fabp3 KO mice, respectively. H, I, Quantitative analysis of the DA and 5-HT concentrations in ACC extracts by ELISA. 5-HT, n = 6 mice per group; DA, n = 6 and 5 WT and Fabp3 KO mice, respectively. A–I, **p < 0.01 versus WT mice. J, Left, Representative immunoblots probed with FABP3 antibody. Right, Quantitative analysis of Gad67 mRNA in Neuro-2a cells. n = 4 per group. **p < 0.01 versus control. mock, Mock control; OE, Fabp3 overexpression; NC, negative control; KD, Fabp3 knockdown. Error bar indicates mean ± SEM.

    Techniques Used: Expressing, Western Blot, Enzyme-linked Immunosorbent Assay, Over Expression, Negative Control

    Enhancement of inhibitory synaptic plasticity in the ACC of Fabp3 KO mice. A, Representative traces of mEPSCs and mIPSCs in ACC layer II/III pyramidal neurons. B, Plots of the mEPSC and mIPSC frequencies in WT and Fabp3 KO mice. C, Plots of the mEPSC and mIPSC amplitudes in WT and Fabp3 KO mice. n = 53 cells for WT and 59 cells for Fabp3 KO from 5 mice for mEPSCs. n = 52 cells for WT and 60 cells for Fabp3 KO from 5 mice for mIPSCs. **p < 0.01 versus WT mice. Error bar indicates mean ± SEM.
    Figure Legend Snippet: Enhancement of inhibitory synaptic plasticity in the ACC of Fabp3 KO mice. A, Representative traces of mEPSCs and mIPSCs in ACC layer II/III pyramidal neurons. B, Plots of the mEPSC and mIPSC frequencies in WT and Fabp3 KO mice. C, Plots of the mEPSC and mIPSC amplitudes in WT and Fabp3 KO mice. n = 53 cells for WT and 59 cells for Fabp3 KO from 5 mice for mEPSCs. n = 52 cells for WT and 60 cells for Fabp3 KO from 5 mice for mIPSCs. **p < 0.01 versus WT mice. Error bar indicates mean ± SEM.

    Techniques Used:

    Extracellular glutamate (Glu) concentrations in the ACC in freely moving animals. A, Dialysate Glu concentrations at baseline (basal) and after depolarization stimulation (High-K+) (left). Glu AUC before (basal) and after (High-K+) depolarization stimulation (right). *p < 0.05 versus wild-type mice. B, Normalization of the dialysate Glu signals indicated that the response to depolarization stimulation was enhanced in Fabp3 KO mice (left). The normalized Glu AUC after depolarization stimulation (right). A, B, Error bar indicates mean ± SEM. *p < 0.05 versus WT mice. n = 6 WT mice; n = 5 Fabp3 KO mice. C, D, Representative immunoblots (left) and quantitative densitometry analysis (right) probed with various antibodies. *p < 0.05; **p < 0.01 versus WT mice. n = 6 mice per group. Error bar indicates mean ± SEM.
    Figure Legend Snippet: Extracellular glutamate (Glu) concentrations in the ACC in freely moving animals. A, Dialysate Glu concentrations at baseline (basal) and after depolarization stimulation (High-K+) (left). Glu AUC before (basal) and after (High-K+) depolarization stimulation (right). *p < 0.05 versus wild-type mice. B, Normalization of the dialysate Glu signals indicated that the response to depolarization stimulation was enhanced in Fabp3 KO mice (left). The normalized Glu AUC after depolarization stimulation (right). A, B, Error bar indicates mean ± SEM. *p < 0.05 versus WT mice. n = 6 WT mice; n = 5 Fabp3 KO mice. C, D, Representative immunoblots (left) and quantitative densitometry analysis (right) probed with various antibodies. *p < 0.05; **p < 0.01 versus WT mice. n = 6 mice per group. Error bar indicates mean ± SEM.

    Techniques Used: Western Blot

    Decreased binding of MeCP2 and HDAC1 to the Gad67 promoter region in the ACC of Fabp3 KO mice. A, Representative immunoblots (top) and quantitative densitometry analysis (bottom) probed with various antibodies are shown. n = 6 mice per group. B, ChIP assay of ACC extracts from WT or Fabp3 KO mice via anti-MeCP2 and HDAC1. The precipitated DNA was analyzed by qPCR with primers amplifying the Gad67 promoter region. **p < 0.01 versus WT mice. n = 5 WT mice. n = 4 Fabp3 KO mice. C, DNA methylation profile in the Gad67 promoter region determined by bisulfite sequencing. Black circles represent methylated CpGs. Open circles represent unmethylated sites. Top numbers indicate global percentages of methylated cytosines. D, Quantitative analysis of the Gad67 mRNA expression in the ACC. WT, n = 6; KO, n = 5; KO + MET, n = 6 mice per group. **p < 0.01 versus WT mice. ##p < 0.01 versus KO mice. E, F, Quantitative analysis of the SAM concentration by ELISA. n = 4 per group. **p < 0.01 versus mock control. MET treatment (5.2 mmol/kg, s.c., twice per day for 6 d). mock, Mock control; OE, Fabp3 overexpression. Error bar indicates mean ± SEM.
    Figure Legend Snippet: Decreased binding of MeCP2 and HDAC1 to the Gad67 promoter region in the ACC of Fabp3 KO mice. A, Representative immunoblots (top) and quantitative densitometry analysis (bottom) probed with various antibodies are shown. n = 6 mice per group. B, ChIP assay of ACC extracts from WT or Fabp3 KO mice via anti-MeCP2 and HDAC1. The precipitated DNA was analyzed by qPCR with primers amplifying the Gad67 promoter region. **p < 0.01 versus WT mice. n = 5 WT mice. n = 4 Fabp3 KO mice. C, DNA methylation profile in the Gad67 promoter region determined by bisulfite sequencing. Black circles represent methylated CpGs. Open circles represent unmethylated sites. Top numbers indicate global percentages of methylated cytosines. D, Quantitative analysis of the Gad67 mRNA expression in the ACC. WT, n = 6; KO, n = 5; KO + MET, n = 6 mice per group. **p < 0.01 versus WT mice. ##p < 0.01 versus KO mice. E, F, Quantitative analysis of the SAM concentration by ELISA. n = 4 per group. **p < 0.01 versus mock control. MET treatment (5.2 mmol/kg, s.c., twice per day for 6 d). mock, Mock control; OE, Fabp3 overexpression. Error bar indicates mean ± SEM.

    Techniques Used: Binding Assay, Western Blot, DNA Methylation Assay, Methylation Sequencing, Methylation, Expressing, Concentration Assay, Enzyme-linked Immunosorbent Assay, Over Expression

    Amelioration of behavioral abnormalities in Fabp3 KO mice by chronic treatment with MET. A, Schematic of the behavioral tests and MET treatment (5.2 mmol/kg, s.c., twice per day). During the behavioral testing period, mice received saline or MET once per day after testing. B, OFT. Graph represents the time spent in the center area over a 5 min period for WT and Fabp3 KO mice. n = 14–17 mice per group. C, HBT. An illustrative example of the travel pathway in the HBT using video tracking software (top). Graphs represent the time spent in the center area, total number of head-dips, head-dip duration, and latency to first head-dip for the 5 min testing session (bottom). n = 13–15 mice per group. **p < 0.01 versus saline-treated WT mice. ##p < 0.01 versus saline-treated KO mice. D, NORT. Differences in exploratory preference were assessed between groups in the training (left) or test (right) sessions. n = 11–15 mice per group. **p < 0.01 versus familiar group. Sal, Saline treatment. MET treatment (5.2 mmol/kg, s.c., twice per day for 6 d). Error bar indicates mean ± SEM.
    Figure Legend Snippet: Amelioration of behavioral abnormalities in Fabp3 KO mice by chronic treatment with MET. A, Schematic of the behavioral tests and MET treatment (5.2 mmol/kg, s.c., twice per day). During the behavioral testing period, mice received saline or MET once per day after testing. B, OFT. Graph represents the time spent in the center area over a 5 min period for WT and Fabp3 KO mice. n = 14–17 mice per group. C, HBT. An illustrative example of the travel pathway in the HBT using video tracking software (top). Graphs represent the time spent in the center area, total number of head-dips, head-dip duration, and latency to first head-dip for the 5 min testing session (bottom). n = 13–15 mice per group. **p < 0.01 versus saline-treated WT mice. ##p < 0.01 versus saline-treated KO mice. D, NORT. Differences in exploratory preference were assessed between groups in the training (left) or test (right) sessions. n = 11–15 mice per group. **p < 0.01 versus familiar group. Sal, Saline treatment. MET treatment (5.2 mmol/kg, s.c., twice per day for 6 d). Error bar indicates mean ± SEM.

    Techniques Used: Software

    Locomotor activities during behavioral tests a
    Figure Legend Snippet: Locomotor activities during behavioral tests a

    Techniques Used:

    mouse monoclonal antibodies against fabp3  (Hycult Biotech)


    Bioz Verified Symbol Hycult Biotech is a verified supplier
    Bioz Manufacturer Symbol Hycult Biotech manufactures this product  
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    Structured Review

    Hycult Biotech mouse monoclonal antibodies against fabp3
    <t>FABP3</t> was strongly expressed in PV+ GABAergic interneurons of the ACC. A, Left, RT-PCR analysis of Fabp3 in different brain areas. Right, qPCR analysis of Fabp3 in different brain areas. Error bar indicates mean ± SEM. n = 6 mice per group. **p < 0.01 versus WT ACC. B–F, Confocal images showing colocalization of FABP3 and a pyramidal neuronal marker (Ng), three classical GABAergic neuronal makers (PV, SOM, and CR), or GAD67 in the ACC. B, Most FABP3+ structures do not show immunoreactivity for Ng. C, Confocal images showing FABP3 and PV colocalization in the ACC. D, F, Most FABP+ structures do not show immunoreactivity for SOM (D) or CR (E). F, Confocal images showing FABP3 and GAD67 colocalization in the ACC (arrowheads). B, C (bottom), D, E (bottom right), F (right), Enlarged images of the boxed area in the merged image. Scale bars, 50 μm. Str, Striatum.
    Mouse Monoclonal Antibodies Against Fabp3, supplied by Hycult Biotech, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mouse monoclonal antibodies against fabp3/product/Hycult Biotech
    Average 91 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    mouse monoclonal antibodies against fabp3 - by Bioz Stars, 2024-04
    91/100 stars

    Images

    1) Product Images from "FABP3 in the Anterior Cingulate Cortex Modulates the Methylation Status of the Glutamic Acid Decarboxylase 67 Promoter Region"

    Article Title: FABP3 in the Anterior Cingulate Cortex Modulates the Methylation Status of the Glutamic Acid Decarboxylase 67 Promoter Region

    Journal: The Journal of Neuroscience

    doi: 10.1523/JNEUROSCI.1285-18.2018

    FABP3 was strongly expressed in PV+ GABAergic interneurons of the ACC. A, Left, RT-PCR analysis of Fabp3 in different brain areas. Right, qPCR analysis of Fabp3 in different brain areas. Error bar indicates mean ± SEM. n = 6 mice per group. **p < 0.01 versus WT ACC. B–F, Confocal images showing colocalization of FABP3 and a pyramidal neuronal marker (Ng), three classical GABAergic neuronal makers (PV, SOM, and CR), or GAD67 in the ACC. B, Most FABP3+ structures do not show immunoreactivity for Ng. C, Confocal images showing FABP3 and PV colocalization in the ACC. D, F, Most FABP+ structures do not show immunoreactivity for SOM (D) or CR (E). F, Confocal images showing FABP3 and GAD67 colocalization in the ACC (arrowheads). B, C (bottom), D, E (bottom right), F (right), Enlarged images of the boxed area in the merged image. Scale bars, 50 μm. Str, Striatum.
    Figure Legend Snippet: FABP3 was strongly expressed in PV+ GABAergic interneurons of the ACC. A, Left, RT-PCR analysis of Fabp3 in different brain areas. Right, qPCR analysis of Fabp3 in different brain areas. Error bar indicates mean ± SEM. n = 6 mice per group. **p < 0.01 versus WT ACC. B–F, Confocal images showing colocalization of FABP3 and a pyramidal neuronal marker (Ng), three classical GABAergic neuronal makers (PV, SOM, and CR), or GAD67 in the ACC. B, Most FABP3+ structures do not show immunoreactivity for Ng. C, Confocal images showing FABP3 and PV colocalization in the ACC. D, F, Most FABP+ structures do not show immunoreactivity for SOM (D) or CR (E). F, Confocal images showing FABP3 and GAD67 colocalization in the ACC (arrowheads). B, C (bottom), D, E (bottom right), F (right), Enlarged images of the boxed area in the merged image. Scale bars, 50 μm. Str, Striatum.

    Techniques Used: Reverse Transcription Polymerase Chain Reaction, Marker

    Colocalization of PV, SOM, and CR with  FABP3  in the ACC a
    Figure Legend Snippet: Colocalization of PV, SOM, and CR with FABP3 in the ACC a

    Techniques Used:

    Fabp3 gene ablation in the ACC caused upregulation of GAD67 expression and GABA synthesis. A, Quantitative analysis of the number of PV−, SOM−, and CR+ neurons in the ACC. PV, n = 8 sections, 4 mice; SOM, n = 12 sections, 6 mice; CR, n = 12 sections, 6 mice per group. B, Representative immunoblots (left) and quantitative densitometry analysis (right) probed with GABA-related antibodies. GAD67 and β-actin, n = 6 mice per group; GAD65, VGAT, and GABAAR, n = 5 mice per group; gephyrin, n = 5 and 6 WT and Fabp3 KO mice, respectively. C, Quantitative analysis of Gad67 mRNA expression in the ACC from WT and Fabp3 KO mice. n = 4 mice per group. D, Quantitative analysis of the GABA concentrations in ACC extracts from WT and Fabp3 KO mice by ELISA. n = 6 mice per group. E–G, There were no differences in GAD67 expression or GABA synthesis in the mPFC or AM of Fabp3 KO mice. E, Representative immunoblots (left) and quantitative densitometry analysis (right) probed with GAD67 antibody. n = 4 mice per group. F, Quantitative analysis of the Gad67 mRNA expression. n = 4 mice per group. G, Quantitative analysis of the GABA concentrations in the mPFC and AM extracts by ELISA. mPFC, n = 6 mice per group; AM, n = 4 and 6 WT and Fabp3 KO mice, respectively. H, I, Quantitative analysis of the DA and 5-HT concentrations in ACC extracts by ELISA. 5-HT, n = 6 mice per group; DA, n = 6 and 5 WT and Fabp3 KO mice, respectively. A–I, **p < 0.01 versus WT mice. J, Left, Representative immunoblots probed with FABP3 antibody. Right, Quantitative analysis of Gad67 mRNA in Neuro-2a cells. n = 4 per group. **p < 0.01 versus control. mock, Mock control; OE, Fabp3 overexpression; NC, negative control; KD, Fabp3 knockdown. Error bar indicates mean ± SEM.
    Figure Legend Snippet: Fabp3 gene ablation in the ACC caused upregulation of GAD67 expression and GABA synthesis. A, Quantitative analysis of the number of PV−, SOM−, and CR+ neurons in the ACC. PV, n = 8 sections, 4 mice; SOM, n = 12 sections, 6 mice; CR, n = 12 sections, 6 mice per group. B, Representative immunoblots (left) and quantitative densitometry analysis (right) probed with GABA-related antibodies. GAD67 and β-actin, n = 6 mice per group; GAD65, VGAT, and GABAAR, n = 5 mice per group; gephyrin, n = 5 and 6 WT and Fabp3 KO mice, respectively. C, Quantitative analysis of Gad67 mRNA expression in the ACC from WT and Fabp3 KO mice. n = 4 mice per group. D, Quantitative analysis of the GABA concentrations in ACC extracts from WT and Fabp3 KO mice by ELISA. n = 6 mice per group. E–G, There were no differences in GAD67 expression or GABA synthesis in the mPFC or AM of Fabp3 KO mice. E, Representative immunoblots (left) and quantitative densitometry analysis (right) probed with GAD67 antibody. n = 4 mice per group. F, Quantitative analysis of the Gad67 mRNA expression. n = 4 mice per group. G, Quantitative analysis of the GABA concentrations in the mPFC and AM extracts by ELISA. mPFC, n = 6 mice per group; AM, n = 4 and 6 WT and Fabp3 KO mice, respectively. H, I, Quantitative analysis of the DA and 5-HT concentrations in ACC extracts by ELISA. 5-HT, n = 6 mice per group; DA, n = 6 and 5 WT and Fabp3 KO mice, respectively. A–I, **p < 0.01 versus WT mice. J, Left, Representative immunoblots probed with FABP3 antibody. Right, Quantitative analysis of Gad67 mRNA in Neuro-2a cells. n = 4 per group. **p < 0.01 versus control. mock, Mock control; OE, Fabp3 overexpression; NC, negative control; KD, Fabp3 knockdown. Error bar indicates mean ± SEM.

    Techniques Used: Expressing, Western Blot, Enzyme-linked Immunosorbent Assay, Over Expression, Negative Control

    Enhancement of inhibitory synaptic plasticity in the ACC of Fabp3 KO mice. A, Representative traces of mEPSCs and mIPSCs in ACC layer II/III pyramidal neurons. B, Plots of the mEPSC and mIPSC frequencies in WT and Fabp3 KO mice. C, Plots of the mEPSC and mIPSC amplitudes in WT and Fabp3 KO mice. n = 53 cells for WT and 59 cells for Fabp3 KO from 5 mice for mEPSCs. n = 52 cells for WT and 60 cells for Fabp3 KO from 5 mice for mIPSCs. **p < 0.01 versus WT mice. Error bar indicates mean ± SEM.
    Figure Legend Snippet: Enhancement of inhibitory synaptic plasticity in the ACC of Fabp3 KO mice. A, Representative traces of mEPSCs and mIPSCs in ACC layer II/III pyramidal neurons. B, Plots of the mEPSC and mIPSC frequencies in WT and Fabp3 KO mice. C, Plots of the mEPSC and mIPSC amplitudes in WT and Fabp3 KO mice. n = 53 cells for WT and 59 cells for Fabp3 KO from 5 mice for mEPSCs. n = 52 cells for WT and 60 cells for Fabp3 KO from 5 mice for mIPSCs. **p < 0.01 versus WT mice. Error bar indicates mean ± SEM.

    Techniques Used:

    Extracellular glutamate (Glu) concentrations in the ACC in freely moving animals. A, Dialysate Glu concentrations at baseline (basal) and after depolarization stimulation (High-K+) (left). Glu AUC before (basal) and after (High-K+) depolarization stimulation (right). *p < 0.05 versus wild-type mice. B, Normalization of the dialysate Glu signals indicated that the response to depolarization stimulation was enhanced in Fabp3 KO mice (left). The normalized Glu AUC after depolarization stimulation (right). A, B, Error bar indicates mean ± SEM. *p < 0.05 versus WT mice. n = 6 WT mice; n = 5 Fabp3 KO mice. C, D, Representative immunoblots (left) and quantitative densitometry analysis (right) probed with various antibodies. *p < 0.05; **p < 0.01 versus WT mice. n = 6 mice per group. Error bar indicates mean ± SEM.
    Figure Legend Snippet: Extracellular glutamate (Glu) concentrations in the ACC in freely moving animals. A, Dialysate Glu concentrations at baseline (basal) and after depolarization stimulation (High-K+) (left). Glu AUC before (basal) and after (High-K+) depolarization stimulation (right). *p < 0.05 versus wild-type mice. B, Normalization of the dialysate Glu signals indicated that the response to depolarization stimulation was enhanced in Fabp3 KO mice (left). The normalized Glu AUC after depolarization stimulation (right). A, B, Error bar indicates mean ± SEM. *p < 0.05 versus WT mice. n = 6 WT mice; n = 5 Fabp3 KO mice. C, D, Representative immunoblots (left) and quantitative densitometry analysis (right) probed with various antibodies. *p < 0.05; **p < 0.01 versus WT mice. n = 6 mice per group. Error bar indicates mean ± SEM.

    Techniques Used: Western Blot

    Decreased binding of MeCP2 and HDAC1 to the Gad67 promoter region in the ACC of Fabp3 KO mice. A, Representative immunoblots (top) and quantitative densitometry analysis (bottom) probed with various antibodies are shown. n = 6 mice per group. B, ChIP assay of ACC extracts from WT or Fabp3 KO mice via anti-MeCP2 and HDAC1. The precipitated DNA was analyzed by qPCR with primers amplifying the Gad67 promoter region. **p < 0.01 versus WT mice. n = 5 WT mice. n = 4 Fabp3 KO mice. C, DNA methylation profile in the Gad67 promoter region determined by bisulfite sequencing. Black circles represent methylated CpGs. Open circles represent unmethylated sites. Top numbers indicate global percentages of methylated cytosines. D, Quantitative analysis of the Gad67 mRNA expression in the ACC. WT, n = 6; KO, n = 5; KO + MET, n = 6 mice per group. **p < 0.01 versus WT mice. ##p < 0.01 versus KO mice. E, F, Quantitative analysis of the SAM concentration by ELISA. n = 4 per group. **p < 0.01 versus mock control. MET treatment (5.2 mmol/kg, s.c., twice per day for 6 d). mock, Mock control; OE, Fabp3 overexpression. Error bar indicates mean ± SEM.
    Figure Legend Snippet: Decreased binding of MeCP2 and HDAC1 to the Gad67 promoter region in the ACC of Fabp3 KO mice. A, Representative immunoblots (top) and quantitative densitometry analysis (bottom) probed with various antibodies are shown. n = 6 mice per group. B, ChIP assay of ACC extracts from WT or Fabp3 KO mice via anti-MeCP2 and HDAC1. The precipitated DNA was analyzed by qPCR with primers amplifying the Gad67 promoter region. **p < 0.01 versus WT mice. n = 5 WT mice. n = 4 Fabp3 KO mice. C, DNA methylation profile in the Gad67 promoter region determined by bisulfite sequencing. Black circles represent methylated CpGs. Open circles represent unmethylated sites. Top numbers indicate global percentages of methylated cytosines. D, Quantitative analysis of the Gad67 mRNA expression in the ACC. WT, n = 6; KO, n = 5; KO + MET, n = 6 mice per group. **p < 0.01 versus WT mice. ##p < 0.01 versus KO mice. E, F, Quantitative analysis of the SAM concentration by ELISA. n = 4 per group. **p < 0.01 versus mock control. MET treatment (5.2 mmol/kg, s.c., twice per day for 6 d). mock, Mock control; OE, Fabp3 overexpression. Error bar indicates mean ± SEM.

    Techniques Used: Binding Assay, Western Blot, DNA Methylation Assay, Methylation Sequencing, Methylation, Expressing, Concentration Assay, Enzyme-linked Immunosorbent Assay, Over Expression

    Amelioration of behavioral abnormalities in Fabp3 KO mice by chronic treatment with MET. A, Schematic of the behavioral tests and MET treatment (5.2 mmol/kg, s.c., twice per day). During the behavioral testing period, mice received saline or MET once per day after testing. B, OFT. Graph represents the time spent in the center area over a 5 min period for WT and Fabp3 KO mice. n = 14–17 mice per group. C, HBT. An illustrative example of the travel pathway in the HBT using video tracking software (top). Graphs represent the time spent in the center area, total number of head-dips, head-dip duration, and latency to first head-dip for the 5 min testing session (bottom). n = 13–15 mice per group. **p < 0.01 versus saline-treated WT mice. ##p < 0.01 versus saline-treated KO mice. D, NORT. Differences in exploratory preference were assessed between groups in the training (left) or test (right) sessions. n = 11–15 mice per group. **p < 0.01 versus familiar group. Sal, Saline treatment. MET treatment (5.2 mmol/kg, s.c., twice per day for 6 d). Error bar indicates mean ± SEM.
    Figure Legend Snippet: Amelioration of behavioral abnormalities in Fabp3 KO mice by chronic treatment with MET. A, Schematic of the behavioral tests and MET treatment (5.2 mmol/kg, s.c., twice per day). During the behavioral testing period, mice received saline or MET once per day after testing. B, OFT. Graph represents the time spent in the center area over a 5 min period for WT and Fabp3 KO mice. n = 14–17 mice per group. C, HBT. An illustrative example of the travel pathway in the HBT using video tracking software (top). Graphs represent the time spent in the center area, total number of head-dips, head-dip duration, and latency to first head-dip for the 5 min testing session (bottom). n = 13–15 mice per group. **p < 0.01 versus saline-treated WT mice. ##p < 0.01 versus saline-treated KO mice. D, NORT. Differences in exploratory preference were assessed between groups in the training (left) or test (right) sessions. n = 11–15 mice per group. **p < 0.01 versus familiar group. Sal, Saline treatment. MET treatment (5.2 mmol/kg, s.c., twice per day for 6 d). Error bar indicates mean ± SEM.

    Techniques Used: Software

    Locomotor activities during behavioral tests a
    Figure Legend Snippet: Locomotor activities during behavioral tests a

    Techniques Used:

    mouse monoclonal antibodies against fabp3  (Hycult Biotech)


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    Hycult Biotech mouse monoclonal antibodies against fabp3
    <t>FABP3</t> was strongly expressed in PV+ GABAergic interneurons of the ACC. A, Left, RT-PCR analysis of Fabp3 in different brain areas. Right, qPCR analysis of Fabp3 in different brain areas. Error bar indicates mean ± SEM. n = 6 mice per group. **p < 0.01 versus WT ACC. B–F, Confocal images showing colocalization of FABP3 and a pyramidal neuronal marker (Ng), three classical GABAergic neuronal makers (PV, SOM, and CR), or GAD67 in the ACC. B, Most FABP3+ structures do not show immunoreactivity for Ng. C, Confocal images showing FABP3 and PV colocalization in the ACC. D, F, Most FABP+ structures do not show immunoreactivity for SOM (D) or CR (E). F, Confocal images showing FABP3 and GAD67 colocalization in the ACC (arrowheads). B, C (bottom), D, E (bottom right), F (right), Enlarged images of the boxed area in the merged image. Scale bars, 50 μm. Str, Striatum.
    Mouse Monoclonal Antibodies Against Fabp3, supplied by Hycult Biotech, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Images

    1) Product Images from "FABP3 in the Anterior Cingulate Cortex Modulates the Methylation Status of the Glutamic Acid Decarboxylase 67 Promoter Region"

    Article Title: FABP3 in the Anterior Cingulate Cortex Modulates the Methylation Status of the Glutamic Acid Decarboxylase 67 Promoter Region

    Journal: The Journal of Neuroscience

    doi: 10.1523/JNEUROSCI.1285-18.2018

    FABP3 was strongly expressed in PV+ GABAergic interneurons of the ACC. A, Left, RT-PCR analysis of Fabp3 in different brain areas. Right, qPCR analysis of Fabp3 in different brain areas. Error bar indicates mean ± SEM. n = 6 mice per group. **p < 0.01 versus WT ACC. B–F, Confocal images showing colocalization of FABP3 and a pyramidal neuronal marker (Ng), three classical GABAergic neuronal makers (PV, SOM, and CR), or GAD67 in the ACC. B, Most FABP3+ structures do not show immunoreactivity for Ng. C, Confocal images showing FABP3 and PV colocalization in the ACC. D, F, Most FABP+ structures do not show immunoreactivity for SOM (D) or CR (E). F, Confocal images showing FABP3 and GAD67 colocalization in the ACC (arrowheads). B, C (bottom), D, E (bottom right), F (right), Enlarged images of the boxed area in the merged image. Scale bars, 50 μm. Str, Striatum.
    Figure Legend Snippet: FABP3 was strongly expressed in PV+ GABAergic interneurons of the ACC. A, Left, RT-PCR analysis of Fabp3 in different brain areas. Right, qPCR analysis of Fabp3 in different brain areas. Error bar indicates mean ± SEM. n = 6 mice per group. **p < 0.01 versus WT ACC. B–F, Confocal images showing colocalization of FABP3 and a pyramidal neuronal marker (Ng), three classical GABAergic neuronal makers (PV, SOM, and CR), or GAD67 in the ACC. B, Most FABP3+ structures do not show immunoreactivity for Ng. C, Confocal images showing FABP3 and PV colocalization in the ACC. D, F, Most FABP+ structures do not show immunoreactivity for SOM (D) or CR (E). F, Confocal images showing FABP3 and GAD67 colocalization in the ACC (arrowheads). B, C (bottom), D, E (bottom right), F (right), Enlarged images of the boxed area in the merged image. Scale bars, 50 μm. Str, Striatum.

    Techniques Used: Reverse Transcription Polymerase Chain Reaction, Marker

    Colocalization of PV, SOM, and CR with  FABP3  in the ACC a
    Figure Legend Snippet: Colocalization of PV, SOM, and CR with FABP3 in the ACC a

    Techniques Used:

    Fabp3 gene ablation in the ACC caused upregulation of GAD67 expression and GABA synthesis. A, Quantitative analysis of the number of PV−, SOM−, and CR+ neurons in the ACC. PV, n = 8 sections, 4 mice; SOM, n = 12 sections, 6 mice; CR, n = 12 sections, 6 mice per group. B, Representative immunoblots (left) and quantitative densitometry analysis (right) probed with GABA-related antibodies. GAD67 and β-actin, n = 6 mice per group; GAD65, VGAT, and GABAAR, n = 5 mice per group; gephyrin, n = 5 and 6 WT and Fabp3 KO mice, respectively. C, Quantitative analysis of Gad67 mRNA expression in the ACC from WT and Fabp3 KO mice. n = 4 mice per group. D, Quantitative analysis of the GABA concentrations in ACC extracts from WT and Fabp3 KO mice by ELISA. n = 6 mice per group. E–G, There were no differences in GAD67 expression or GABA synthesis in the mPFC or AM of Fabp3 KO mice. E, Representative immunoblots (left) and quantitative densitometry analysis (right) probed with GAD67 antibody. n = 4 mice per group. F, Quantitative analysis of the Gad67 mRNA expression. n = 4 mice per group. G, Quantitative analysis of the GABA concentrations in the mPFC and AM extracts by ELISA. mPFC, n = 6 mice per group; AM, n = 4 and 6 WT and Fabp3 KO mice, respectively. H, I, Quantitative analysis of the DA and 5-HT concentrations in ACC extracts by ELISA. 5-HT, n = 6 mice per group; DA, n = 6 and 5 WT and Fabp3 KO mice, respectively. A–I, **p < 0.01 versus WT mice. J, Left, Representative immunoblots probed with FABP3 antibody. Right, Quantitative analysis of Gad67 mRNA in Neuro-2a cells. n = 4 per group. **p < 0.01 versus control. mock, Mock control; OE, Fabp3 overexpression; NC, negative control; KD, Fabp3 knockdown. Error bar indicates mean ± SEM.
    Figure Legend Snippet: Fabp3 gene ablation in the ACC caused upregulation of GAD67 expression and GABA synthesis. A, Quantitative analysis of the number of PV−, SOM−, and CR+ neurons in the ACC. PV, n = 8 sections, 4 mice; SOM, n = 12 sections, 6 mice; CR, n = 12 sections, 6 mice per group. B, Representative immunoblots (left) and quantitative densitometry analysis (right) probed with GABA-related antibodies. GAD67 and β-actin, n = 6 mice per group; GAD65, VGAT, and GABAAR, n = 5 mice per group; gephyrin, n = 5 and 6 WT and Fabp3 KO mice, respectively. C, Quantitative analysis of Gad67 mRNA expression in the ACC from WT and Fabp3 KO mice. n = 4 mice per group. D, Quantitative analysis of the GABA concentrations in ACC extracts from WT and Fabp3 KO mice by ELISA. n = 6 mice per group. E–G, There were no differences in GAD67 expression or GABA synthesis in the mPFC or AM of Fabp3 KO mice. E, Representative immunoblots (left) and quantitative densitometry analysis (right) probed with GAD67 antibody. n = 4 mice per group. F, Quantitative analysis of the Gad67 mRNA expression. n = 4 mice per group. G, Quantitative analysis of the GABA concentrations in the mPFC and AM extracts by ELISA. mPFC, n = 6 mice per group; AM, n = 4 and 6 WT and Fabp3 KO mice, respectively. H, I, Quantitative analysis of the DA and 5-HT concentrations in ACC extracts by ELISA. 5-HT, n = 6 mice per group; DA, n = 6 and 5 WT and Fabp3 KO mice, respectively. A–I, **p < 0.01 versus WT mice. J, Left, Representative immunoblots probed with FABP3 antibody. Right, Quantitative analysis of Gad67 mRNA in Neuro-2a cells. n = 4 per group. **p < 0.01 versus control. mock, Mock control; OE, Fabp3 overexpression; NC, negative control; KD, Fabp3 knockdown. Error bar indicates mean ± SEM.

    Techniques Used: Expressing, Western Blot, Enzyme-linked Immunosorbent Assay, Over Expression, Negative Control

    Enhancement of inhibitory synaptic plasticity in the ACC of Fabp3 KO mice. A, Representative traces of mEPSCs and mIPSCs in ACC layer II/III pyramidal neurons. B, Plots of the mEPSC and mIPSC frequencies in WT and Fabp3 KO mice. C, Plots of the mEPSC and mIPSC amplitudes in WT and Fabp3 KO mice. n = 53 cells for WT and 59 cells for Fabp3 KO from 5 mice for mEPSCs. n = 52 cells for WT and 60 cells for Fabp3 KO from 5 mice for mIPSCs. **p < 0.01 versus WT mice. Error bar indicates mean ± SEM.
    Figure Legend Snippet: Enhancement of inhibitory synaptic plasticity in the ACC of Fabp3 KO mice. A, Representative traces of mEPSCs and mIPSCs in ACC layer II/III pyramidal neurons. B, Plots of the mEPSC and mIPSC frequencies in WT and Fabp3 KO mice. C, Plots of the mEPSC and mIPSC amplitudes in WT and Fabp3 KO mice. n = 53 cells for WT and 59 cells for Fabp3 KO from 5 mice for mEPSCs. n = 52 cells for WT and 60 cells for Fabp3 KO from 5 mice for mIPSCs. **p < 0.01 versus WT mice. Error bar indicates mean ± SEM.

    Techniques Used:

    Extracellular glutamate (Glu) concentrations in the ACC in freely moving animals. A, Dialysate Glu concentrations at baseline (basal) and after depolarization stimulation (High-K+) (left). Glu AUC before (basal) and after (High-K+) depolarization stimulation (right). *p < 0.05 versus wild-type mice. B, Normalization of the dialysate Glu signals indicated that the response to depolarization stimulation was enhanced in Fabp3 KO mice (left). The normalized Glu AUC after depolarization stimulation (right). A, B, Error bar indicates mean ± SEM. *p < 0.05 versus WT mice. n = 6 WT mice; n = 5 Fabp3 KO mice. C, D, Representative immunoblots (left) and quantitative densitometry analysis (right) probed with various antibodies. *p < 0.05; **p < 0.01 versus WT mice. n = 6 mice per group. Error bar indicates mean ± SEM.
    Figure Legend Snippet: Extracellular glutamate (Glu) concentrations in the ACC in freely moving animals. A, Dialysate Glu concentrations at baseline (basal) and after depolarization stimulation (High-K+) (left). Glu AUC before (basal) and after (High-K+) depolarization stimulation (right). *p < 0.05 versus wild-type mice. B, Normalization of the dialysate Glu signals indicated that the response to depolarization stimulation was enhanced in Fabp3 KO mice (left). The normalized Glu AUC after depolarization stimulation (right). A, B, Error bar indicates mean ± SEM. *p < 0.05 versus WT mice. n = 6 WT mice; n = 5 Fabp3 KO mice. C, D, Representative immunoblots (left) and quantitative densitometry analysis (right) probed with various antibodies. *p < 0.05; **p < 0.01 versus WT mice. n = 6 mice per group. Error bar indicates mean ± SEM.

    Techniques Used: Western Blot

    Decreased binding of MeCP2 and HDAC1 to the Gad67 promoter region in the ACC of Fabp3 KO mice. A, Representative immunoblots (top) and quantitative densitometry analysis (bottom) probed with various antibodies are shown. n = 6 mice per group. B, ChIP assay of ACC extracts from WT or Fabp3 KO mice via anti-MeCP2 and HDAC1. The precipitated DNA was analyzed by qPCR with primers amplifying the Gad67 promoter region. **p < 0.01 versus WT mice. n = 5 WT mice. n = 4 Fabp3 KO mice. C, DNA methylation profile in the Gad67 promoter region determined by bisulfite sequencing. Black circles represent methylated CpGs. Open circles represent unmethylated sites. Top numbers indicate global percentages of methylated cytosines. D, Quantitative analysis of the Gad67 mRNA expression in the ACC. WT, n = 6; KO, n = 5; KO + MET, n = 6 mice per group. **p < 0.01 versus WT mice. ##p < 0.01 versus KO mice. E, F, Quantitative analysis of the SAM concentration by ELISA. n = 4 per group. **p < 0.01 versus mock control. MET treatment (5.2 mmol/kg, s.c., twice per day for 6 d). mock, Mock control; OE, Fabp3 overexpression. Error bar indicates mean ± SEM.
    Figure Legend Snippet: Decreased binding of MeCP2 and HDAC1 to the Gad67 promoter region in the ACC of Fabp3 KO mice. A, Representative immunoblots (top) and quantitative densitometry analysis (bottom) probed with various antibodies are shown. n = 6 mice per group. B, ChIP assay of ACC extracts from WT or Fabp3 KO mice via anti-MeCP2 and HDAC1. The precipitated DNA was analyzed by qPCR with primers amplifying the Gad67 promoter region. **p < 0.01 versus WT mice. n = 5 WT mice. n = 4 Fabp3 KO mice. C, DNA methylation profile in the Gad67 promoter region determined by bisulfite sequencing. Black circles represent methylated CpGs. Open circles represent unmethylated sites. Top numbers indicate global percentages of methylated cytosines. D, Quantitative analysis of the Gad67 mRNA expression in the ACC. WT, n = 6; KO, n = 5; KO + MET, n = 6 mice per group. **p < 0.01 versus WT mice. ##p < 0.01 versus KO mice. E, F, Quantitative analysis of the SAM concentration by ELISA. n = 4 per group. **p < 0.01 versus mock control. MET treatment (5.2 mmol/kg, s.c., twice per day for 6 d). mock, Mock control; OE, Fabp3 overexpression. Error bar indicates mean ± SEM.

    Techniques Used: Binding Assay, Western Blot, DNA Methylation Assay, Methylation Sequencing, Methylation, Expressing, Concentration Assay, Enzyme-linked Immunosorbent Assay, Over Expression

    Amelioration of behavioral abnormalities in Fabp3 KO mice by chronic treatment with MET. A, Schematic of the behavioral tests and MET treatment (5.2 mmol/kg, s.c., twice per day). During the behavioral testing period, mice received saline or MET once per day after testing. B, OFT. Graph represents the time spent in the center area over a 5 min period for WT and Fabp3 KO mice. n = 14–17 mice per group. C, HBT. An illustrative example of the travel pathway in the HBT using video tracking software (top). Graphs represent the time spent in the center area, total number of head-dips, head-dip duration, and latency to first head-dip for the 5 min testing session (bottom). n = 13–15 mice per group. **p < 0.01 versus saline-treated WT mice. ##p < 0.01 versus saline-treated KO mice. D, NORT. Differences in exploratory preference were assessed between groups in the training (left) or test (right) sessions. n = 11–15 mice per group. **p < 0.01 versus familiar group. Sal, Saline treatment. MET treatment (5.2 mmol/kg, s.c., twice per day for 6 d). Error bar indicates mean ± SEM.
    Figure Legend Snippet: Amelioration of behavioral abnormalities in Fabp3 KO mice by chronic treatment with MET. A, Schematic of the behavioral tests and MET treatment (5.2 mmol/kg, s.c., twice per day). During the behavioral testing period, mice received saline or MET once per day after testing. B, OFT. Graph represents the time spent in the center area over a 5 min period for WT and Fabp3 KO mice. n = 14–17 mice per group. C, HBT. An illustrative example of the travel pathway in the HBT using video tracking software (top). Graphs represent the time spent in the center area, total number of head-dips, head-dip duration, and latency to first head-dip for the 5 min testing session (bottom). n = 13–15 mice per group. **p < 0.01 versus saline-treated WT mice. ##p < 0.01 versus saline-treated KO mice. D, NORT. Differences in exploratory preference were assessed between groups in the training (left) or test (right) sessions. n = 11–15 mice per group. **p < 0.01 versus familiar group. Sal, Saline treatment. MET treatment (5.2 mmol/kg, s.c., twice per day for 6 d). Error bar indicates mean ± SEM.

    Techniques Used: Software

    Locomotor activities during behavioral tests a
    Figure Legend Snippet: Locomotor activities during behavioral tests a

    Techniques Used:

    mouse monoclonal antibodies against fabp3  (Hycult Biotech)


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    Hycult Biotech mouse monoclonal antibodies against fabp3
    Mouse Monoclonal Antibodies Against Fabp3, supplied by Hycult Biotech, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    mouse monoclonal antibodies against fabp3  (Hycult Biotech)


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    Hycult Biotech mouse monoclonal antibodies against fabp3
    Mouse Monoclonal Antibodies Against Fabp3, supplied by Hycult Biotech, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Hycult Biotech anti human monoclonal antibody h fabp 66e2
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    Hycult Biotech monoclonal antibody
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    Hycult Biotech mouse anti fabp3
    Effects of I/R injury on <t>FABP3,</t> FABP5 and FABP7 expression levels in the brain. ( A ) The locations of samples. Brains were cut into four slices (2-mm thick) from the front of the cortex. The second slices (designated as con and ips slices) of the brain, including the cortex and striatum, were used for Western blot analysis and PGE 2 -content analysis in the following experiments. ( B – E ) Mice were subjected to right tMCAO for 2 h. At 6, 12, 24 and 48 h after reperfusion, the second slice of the right brain (ipsilateral) and the left brain (contralateral) areas were collected for Western blot analysis of FABPs levels. ( B ) Representative images of Western blots. Quantitative analyses of FABP3 ( C ), FABP5 ( D ) and FABP7 ( E ) expression levels in contralateral (black) and ipsilateral (blue) areas of the brains. # p < 0.05, ## p < 0.01 vs. the sham group (contralateral); * p < 0.05, ** p < 0.01 vs. the sham group (ipsilateral) (n = 6 per group). Differences were statistically analyzed using one-way analysis of variance (ANOVA) followed by Dunnett’s test.
    Mouse Anti Fabp3, supplied by Hycult Biotech, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Hycult Biotech mouse monoclonal antibodies against fabp3
    <t>FABP3</t> was strongly expressed in PV+ GABAergic interneurons of the ACC. A, Left, RT-PCR analysis of Fabp3 in different brain areas. Right, qPCR analysis of Fabp3 in different brain areas. Error bar indicates mean ± SEM. n = 6 mice per group. **p < 0.01 versus WT ACC. B–F, Confocal images showing colocalization of FABP3 and a pyramidal neuronal marker (Ng), three classical GABAergic neuronal makers (PV, SOM, and CR), or GAD67 in the ACC. B, Most FABP3+ structures do not show immunoreactivity for Ng. C, Confocal images showing FABP3 and PV colocalization in the ACC. D, F, Most FABP+ structures do not show immunoreactivity for SOM (D) or CR (E). F, Confocal images showing FABP3 and GAD67 colocalization in the ACC (arrowheads). B, C (bottom), D, E (bottom right), F (right), Enlarged images of the boxed area in the merged image. Scale bars, 50 μm. Str, Striatum.
    Mouse Monoclonal Antibodies Against Fabp3, supplied by Hycult Biotech, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mouse monoclonal antibodies against fabp3/product/Hycult Biotech
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    Hycult Biotech anti human monoclonal antibody h fabp 66e2
    <t>FABP3</t> was strongly expressed in PV+ GABAergic interneurons of the ACC. A, Left, RT-PCR analysis of Fabp3 in different brain areas. Right, qPCR analysis of Fabp3 in different brain areas. Error bar indicates mean ± SEM. n = 6 mice per group. **p < 0.01 versus WT ACC. B–F, Confocal images showing colocalization of FABP3 and a pyramidal neuronal marker (Ng), three classical GABAergic neuronal makers (PV, SOM, and CR), or GAD67 in the ACC. B, Most FABP3+ structures do not show immunoreactivity for Ng. C, Confocal images showing FABP3 and PV colocalization in the ACC. D, F, Most FABP+ structures do not show immunoreactivity for SOM (D) or CR (E). F, Confocal images showing FABP3 and GAD67 colocalization in the ACC (arrowheads). B, C (bottom), D, E (bottom right), F (right), Enlarged images of the boxed area in the merged image. Scale bars, 50 μm. Str, Striatum.
    Anti Human Monoclonal Antibody H Fabp 66e2, supplied by Hycult Biotech, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Hycult Biotech mouse anti human fatty acid
    <t>FABP3</t> was strongly expressed in PV+ GABAergic interneurons of the ACC. A, Left, RT-PCR analysis of Fabp3 in different brain areas. Right, qPCR analysis of Fabp3 in different brain areas. Error bar indicates mean ± SEM. n = 6 mice per group. **p < 0.01 versus WT ACC. B–F, Confocal images showing colocalization of FABP3 and a pyramidal neuronal marker (Ng), three classical GABAergic neuronal makers (PV, SOM, and CR), or GAD67 in the ACC. B, Most FABP3+ structures do not show immunoreactivity for Ng. C, Confocal images showing FABP3 and PV colocalization in the ACC. D, F, Most FABP+ structures do not show immunoreactivity for SOM (D) or CR (E). F, Confocal images showing FABP3 and GAD67 colocalization in the ACC (arrowheads). B, C (bottom), D, E (bottom right), F (right), Enlarged images of the boxed area in the merged image. Scale bars, 50 μm. Str, Striatum.
    Mouse Anti Human Fatty Acid, supplied by Hycult Biotech, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Hycult Biotech monoclonal antibody
    <t>FABP3</t> was strongly expressed in PV+ GABAergic interneurons of the ACC. A, Left, RT-PCR analysis of Fabp3 in different brain areas. Right, qPCR analysis of Fabp3 in different brain areas. Error bar indicates mean ± SEM. n = 6 mice per group. **p < 0.01 versus WT ACC. B–F, Confocal images showing colocalization of FABP3 and a pyramidal neuronal marker (Ng), three classical GABAergic neuronal makers (PV, SOM, and CR), or GAD67 in the ACC. B, Most FABP3+ structures do not show immunoreactivity for Ng. C, Confocal images showing FABP3 and PV colocalization in the ACC. D, F, Most FABP+ structures do not show immunoreactivity for SOM (D) or CR (E). F, Confocal images showing FABP3 and GAD67 colocalization in the ACC (arrowheads). B, C (bottom), D, E (bottom right), F (right), Enlarged images of the boxed area in the merged image. Scale bars, 50 μm. Str, Striatum.
    Monoclonal Antibody, supplied by Hycult Biotech, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    Effects of I/R injury on FABP3, FABP5 and FABP7 expression levels in the brain. ( A ) The locations of samples. Brains were cut into four slices (2-mm thick) from the front of the cortex. The second slices (designated as con and ips slices) of the brain, including the cortex and striatum, were used for Western blot analysis and PGE 2 -content analysis in the following experiments. ( B – E ) Mice were subjected to right tMCAO for 2 h. At 6, 12, 24 and 48 h after reperfusion, the second slice of the right brain (ipsilateral) and the left brain (contralateral) areas were collected for Western blot analysis of FABPs levels. ( B ) Representative images of Western blots. Quantitative analyses of FABP3 ( C ), FABP5 ( D ) and FABP7 ( E ) expression levels in contralateral (black) and ipsilateral (blue) areas of the brains. # p < 0.05, ## p < 0.01 vs. the sham group (contralateral); * p < 0.05, ** p < 0.01 vs. the sham group (ipsilateral) (n = 6 per group). Differences were statistically analyzed using one-way analysis of variance (ANOVA) followed by Dunnett’s test.

    Journal: Biomedicines

    Article Title: Fatty Acid-Binding Proteins Aggravate Cerebral Ischemia-Reperfusion Injury in Mice

    doi: 10.3390/biomedicines9050529

    Figure Lengend Snippet: Effects of I/R injury on FABP3, FABP5 and FABP7 expression levels in the brain. ( A ) The locations of samples. Brains were cut into four slices (2-mm thick) from the front of the cortex. The second slices (designated as con and ips slices) of the brain, including the cortex and striatum, were used for Western blot analysis and PGE 2 -content analysis in the following experiments. ( B – E ) Mice were subjected to right tMCAO for 2 h. At 6, 12, 24 and 48 h after reperfusion, the second slice of the right brain (ipsilateral) and the left brain (contralateral) areas were collected for Western blot analysis of FABPs levels. ( B ) Representative images of Western blots. Quantitative analyses of FABP3 ( C ), FABP5 ( D ) and FABP7 ( E ) expression levels in contralateral (black) and ipsilateral (blue) areas of the brains. # p < 0.05, ## p < 0.01 vs. the sham group (contralateral); * p < 0.05, ** p < 0.01 vs. the sham group (ipsilateral) (n = 6 per group). Differences were statistically analyzed using one-way analysis of variance (ANOVA) followed by Dunnett’s test.

    Article Snippet: The following working dilutions were used for the indicated monoclonal antibodies, per manufacturer’s suggestions: mouse anti-FABP3 (1:1000; Hycult Biotech, HM2016, Uden, NLD), goat anti-FABP5 (1:1000; R&D Systems, AF3077, Minneapolis, MN, USA), goat anti-FABP7 (1:1000; R&D Systems, AF3166, Minneapolis, MN, USA), rabbit anti-mPGES-1 (1:200; Cayman Chemical, 160140, Ann Arbor, MI, USA) and mouse anti-β-actin (1:5000; Sigma, A5441, St Louis, MO, USA).

    Techniques: Expressing, Western Blot

    Immunofluorescence of FABP3, FABP5 and FABP7 in the cortexes of sham and I/R mice. ( A ) Representative micro-graphs of immunofluorescence staining of the cortical penumbra region (shown in the black box area) at 12 h after reperfusion. ( B ) Double staining for FABP3 (green) and NeuN (a neuronal marker, red) expression in sham mice ( B1 ) and I/R mice ( B2 , ipsilateral). ( C , D ) Double staining for FABP5 (green) and NeuN (red; C ) or Olig2 (an oligodendrocyte marker, red; D ) in sham mice and I/R mice (ipsilateral). ( E , F ) Double staining for FABP7 (green) and GFAP (an as-trocyte marker, red; E ) or Olig2 (red; F ). Scale bar = 50 μm. The two small images on the left show immunofluorescence for FABPs and cell markers, whereas the larger image on the right is a merged image.

    Journal: Biomedicines

    Article Title: Fatty Acid-Binding Proteins Aggravate Cerebral Ischemia-Reperfusion Injury in Mice

    doi: 10.3390/biomedicines9050529

    Figure Lengend Snippet: Immunofluorescence of FABP3, FABP5 and FABP7 in the cortexes of sham and I/R mice. ( A ) Representative micro-graphs of immunofluorescence staining of the cortical penumbra region (shown in the black box area) at 12 h after reperfusion. ( B ) Double staining for FABP3 (green) and NeuN (a neuronal marker, red) expression in sham mice ( B1 ) and I/R mice ( B2 , ipsilateral). ( C , D ) Double staining for FABP5 (green) and NeuN (red; C ) or Olig2 (an oligodendrocyte marker, red; D ) in sham mice and I/R mice (ipsilateral). ( E , F ) Double staining for FABP7 (green) and GFAP (an as-trocyte marker, red; E ) or Olig2 (red; F ). Scale bar = 50 μm. The two small images on the left show immunofluorescence for FABPs and cell markers, whereas the larger image on the right is a merged image.

    Article Snippet: The following working dilutions were used for the indicated monoclonal antibodies, per manufacturer’s suggestions: mouse anti-FABP3 (1:1000; Hycult Biotech, HM2016, Uden, NLD), goat anti-FABP5 (1:1000; R&D Systems, AF3077, Minneapolis, MN, USA), goat anti-FABP7 (1:1000; R&D Systems, AF3166, Minneapolis, MN, USA), rabbit anti-mPGES-1 (1:200; Cayman Chemical, 160140, Ann Arbor, MI, USA) and mouse anti-β-actin (1:5000; Sigma, A5441, St Louis, MO, USA).

    Techniques: Immunofluorescence, Staining, Double Staining, Marker, Expressing

    Effects of MF6 administration on FABP3, FABP5 and FABP7 expression levels in I/R mice. Mice were subjected to right tMCAO for 2 h and administered 3 mg/kg MF6 at 30 min after reperfusion. At 12 h after reperfusion, the second slice of the right (ipsilateral) and left (contralateral) brain areas, including the cortex and striatum, were used for Western blot analysis of FABP levels. ( A ) Representative images of Western blots. Quantitative analyses of FABP3 ( B ), FABP5 ( C ) and FABP7 ( D ) proteins expression levels in the contralateral (black) and ipsilateral (blue) regions of the brains. ** p < 0.01 vs. the sham-treated group, administered the vehicle (contralateral/ipsilateral); # p < 0.05, ## p < 0.01 vs. the I/R-treated group, administered the vehicle (contralateral/ipsilateral) (n = 6 per group). Differences were statistically analyzed using two-way analysis of variance (ANOVA) followed by Student–Newman–Keuls test.

    Journal: Biomedicines

    Article Title: Fatty Acid-Binding Proteins Aggravate Cerebral Ischemia-Reperfusion Injury in Mice

    doi: 10.3390/biomedicines9050529

    Figure Lengend Snippet: Effects of MF6 administration on FABP3, FABP5 and FABP7 expression levels in I/R mice. Mice were subjected to right tMCAO for 2 h and administered 3 mg/kg MF6 at 30 min after reperfusion. At 12 h after reperfusion, the second slice of the right (ipsilateral) and left (contralateral) brain areas, including the cortex and striatum, were used for Western blot analysis of FABP levels. ( A ) Representative images of Western blots. Quantitative analyses of FABP3 ( B ), FABP5 ( C ) and FABP7 ( D ) proteins expression levels in the contralateral (black) and ipsilateral (blue) regions of the brains. ** p < 0.01 vs. the sham-treated group, administered the vehicle (contralateral/ipsilateral); # p < 0.05, ## p < 0.01 vs. the I/R-treated group, administered the vehicle (contralateral/ipsilateral) (n = 6 per group). Differences were statistically analyzed using two-way analysis of variance (ANOVA) followed by Student–Newman–Keuls test.

    Article Snippet: The following working dilutions were used for the indicated monoclonal antibodies, per manufacturer’s suggestions: mouse anti-FABP3 (1:1000; Hycult Biotech, HM2016, Uden, NLD), goat anti-FABP5 (1:1000; R&D Systems, AF3077, Minneapolis, MN, USA), goat anti-FABP7 (1:1000; R&D Systems, AF3166, Minneapolis, MN, USA), rabbit anti-mPGES-1 (1:200; Cayman Chemical, 160140, Ann Arbor, MI, USA) and mouse anti-β-actin (1:5000; Sigma, A5441, St Louis, MO, USA).

    Techniques: Expressing, Western Blot

    FABP3 was strongly expressed in PV+ GABAergic interneurons of the ACC. A, Left, RT-PCR analysis of Fabp3 in different brain areas. Right, qPCR analysis of Fabp3 in different brain areas. Error bar indicates mean ± SEM. n = 6 mice per group. **p < 0.01 versus WT ACC. B–F, Confocal images showing colocalization of FABP3 and a pyramidal neuronal marker (Ng), three classical GABAergic neuronal makers (PV, SOM, and CR), or GAD67 in the ACC. B, Most FABP3+ structures do not show immunoreactivity for Ng. C, Confocal images showing FABP3 and PV colocalization in the ACC. D, F, Most FABP+ structures do not show immunoreactivity for SOM (D) or CR (E). F, Confocal images showing FABP3 and GAD67 colocalization in the ACC (arrowheads). B, C (bottom), D, E (bottom right), F (right), Enlarged images of the boxed area in the merged image. Scale bars, 50 μm. Str, Striatum.

    Journal: The Journal of Neuroscience

    Article Title: FABP3 in the Anterior Cingulate Cortex Modulates the Methylation Status of the Glutamic Acid Decarboxylase 67 Promoter Region

    doi: 10.1523/JNEUROSCI.1285-18.2018

    Figure Lengend Snippet: FABP3 was strongly expressed in PV+ GABAergic interneurons of the ACC. A, Left, RT-PCR analysis of Fabp3 in different brain areas. Right, qPCR analysis of Fabp3 in different brain areas. Error bar indicates mean ± SEM. n = 6 mice per group. **p < 0.01 versus WT ACC. B–F, Confocal images showing colocalization of FABP3 and a pyramidal neuronal marker (Ng), three classical GABAergic neuronal makers (PV, SOM, and CR), or GAD67 in the ACC. B, Most FABP3+ structures do not show immunoreactivity for Ng. C, Confocal images showing FABP3 and PV colocalization in the ACC. D, F, Most FABP+ structures do not show immunoreactivity for SOM (D) or CR (E). F, Confocal images showing FABP3 and GAD67 colocalization in the ACC (arrowheads). B, C (bottom), D, E (bottom right), F (right), Enlarged images of the boxed area in the merged image. Scale bars, 50 μm. Str, Striatum.

    Article Snippet: The primary antibodies included mouse monoclonal antibodies against FABP3 (1:200, Hycult Biotechnology HM2016, RRID: AB_533050 ); rabbit polyclonal antibodies against neurogranin (Ng, 1:2000, Millipore AB5620, RRID: AB_91937 ); rabbit polyclonal antibodies against PV (1:2000, Abcam ab11427, RRID: AB_298032 ); rat monoclonal antibodies against somatostatin (SOM, 1:50, Millipore MAB354, RRID: AB_2255365 ); and rabbit polyclonal antibodies against calretinin (CR, 1:2000, Swant CR7697, RRID: AB_2619710 ).

    Techniques: Reverse Transcription Polymerase Chain Reaction, Marker

    Colocalization of PV, SOM, and CR with  FABP3  in the ACC a

    Journal: The Journal of Neuroscience

    Article Title: FABP3 in the Anterior Cingulate Cortex Modulates the Methylation Status of the Glutamic Acid Decarboxylase 67 Promoter Region

    doi: 10.1523/JNEUROSCI.1285-18.2018

    Figure Lengend Snippet: Colocalization of PV, SOM, and CR with FABP3 in the ACC a

    Article Snippet: The primary antibodies included mouse monoclonal antibodies against FABP3 (1:200, Hycult Biotechnology HM2016, RRID: AB_533050 ); rabbit polyclonal antibodies against neurogranin (Ng, 1:2000, Millipore AB5620, RRID: AB_91937 ); rabbit polyclonal antibodies against PV (1:2000, Abcam ab11427, RRID: AB_298032 ); rat monoclonal antibodies against somatostatin (SOM, 1:50, Millipore MAB354, RRID: AB_2255365 ); and rabbit polyclonal antibodies against calretinin (CR, 1:2000, Swant CR7697, RRID: AB_2619710 ).

    Techniques:

    Fabp3 gene ablation in the ACC caused upregulation of GAD67 expression and GABA synthesis. A, Quantitative analysis of the number of PV−, SOM−, and CR+ neurons in the ACC. PV, n = 8 sections, 4 mice; SOM, n = 12 sections, 6 mice; CR, n = 12 sections, 6 mice per group. B, Representative immunoblots (left) and quantitative densitometry analysis (right) probed with GABA-related antibodies. GAD67 and β-actin, n = 6 mice per group; GAD65, VGAT, and GABAAR, n = 5 mice per group; gephyrin, n = 5 and 6 WT and Fabp3 KO mice, respectively. C, Quantitative analysis of Gad67 mRNA expression in the ACC from WT and Fabp3 KO mice. n = 4 mice per group. D, Quantitative analysis of the GABA concentrations in ACC extracts from WT and Fabp3 KO mice by ELISA. n = 6 mice per group. E–G, There were no differences in GAD67 expression or GABA synthesis in the mPFC or AM of Fabp3 KO mice. E, Representative immunoblots (left) and quantitative densitometry analysis (right) probed with GAD67 antibody. n = 4 mice per group. F, Quantitative analysis of the Gad67 mRNA expression. n = 4 mice per group. G, Quantitative analysis of the GABA concentrations in the mPFC and AM extracts by ELISA. mPFC, n = 6 mice per group; AM, n = 4 and 6 WT and Fabp3 KO mice, respectively. H, I, Quantitative analysis of the DA and 5-HT concentrations in ACC extracts by ELISA. 5-HT, n = 6 mice per group; DA, n = 6 and 5 WT and Fabp3 KO mice, respectively. A–I, **p < 0.01 versus WT mice. J, Left, Representative immunoblots probed with FABP3 antibody. Right, Quantitative analysis of Gad67 mRNA in Neuro-2a cells. n = 4 per group. **p < 0.01 versus control. mock, Mock control; OE, Fabp3 overexpression; NC, negative control; KD, Fabp3 knockdown. Error bar indicates mean ± SEM.

    Journal: The Journal of Neuroscience

    Article Title: FABP3 in the Anterior Cingulate Cortex Modulates the Methylation Status of the Glutamic Acid Decarboxylase 67 Promoter Region

    doi: 10.1523/JNEUROSCI.1285-18.2018

    Figure Lengend Snippet: Fabp3 gene ablation in the ACC caused upregulation of GAD67 expression and GABA synthesis. A, Quantitative analysis of the number of PV−, SOM−, and CR+ neurons in the ACC. PV, n = 8 sections, 4 mice; SOM, n = 12 sections, 6 mice; CR, n = 12 sections, 6 mice per group. B, Representative immunoblots (left) and quantitative densitometry analysis (right) probed with GABA-related antibodies. GAD67 and β-actin, n = 6 mice per group; GAD65, VGAT, and GABAAR, n = 5 mice per group; gephyrin, n = 5 and 6 WT and Fabp3 KO mice, respectively. C, Quantitative analysis of Gad67 mRNA expression in the ACC from WT and Fabp3 KO mice. n = 4 mice per group. D, Quantitative analysis of the GABA concentrations in ACC extracts from WT and Fabp3 KO mice by ELISA. n = 6 mice per group. E–G, There were no differences in GAD67 expression or GABA synthesis in the mPFC or AM of Fabp3 KO mice. E, Representative immunoblots (left) and quantitative densitometry analysis (right) probed with GAD67 antibody. n = 4 mice per group. F, Quantitative analysis of the Gad67 mRNA expression. n = 4 mice per group. G, Quantitative analysis of the GABA concentrations in the mPFC and AM extracts by ELISA. mPFC, n = 6 mice per group; AM, n = 4 and 6 WT and Fabp3 KO mice, respectively. H, I, Quantitative analysis of the DA and 5-HT concentrations in ACC extracts by ELISA. 5-HT, n = 6 mice per group; DA, n = 6 and 5 WT and Fabp3 KO mice, respectively. A–I, **p < 0.01 versus WT mice. J, Left, Representative immunoblots probed with FABP3 antibody. Right, Quantitative analysis of Gad67 mRNA in Neuro-2a cells. n = 4 per group. **p < 0.01 versus control. mock, Mock control; OE, Fabp3 overexpression; NC, negative control; KD, Fabp3 knockdown. Error bar indicates mean ± SEM.

    Article Snippet: The primary antibodies included mouse monoclonal antibodies against FABP3 (1:200, Hycult Biotechnology HM2016, RRID: AB_533050 ); rabbit polyclonal antibodies against neurogranin (Ng, 1:2000, Millipore AB5620, RRID: AB_91937 ); rabbit polyclonal antibodies against PV (1:2000, Abcam ab11427, RRID: AB_298032 ); rat monoclonal antibodies against somatostatin (SOM, 1:50, Millipore MAB354, RRID: AB_2255365 ); and rabbit polyclonal antibodies against calretinin (CR, 1:2000, Swant CR7697, RRID: AB_2619710 ).

    Techniques: Expressing, Western Blot, Enzyme-linked Immunosorbent Assay, Over Expression, Negative Control

    Enhancement of inhibitory synaptic plasticity in the ACC of Fabp3 KO mice. A, Representative traces of mEPSCs and mIPSCs in ACC layer II/III pyramidal neurons. B, Plots of the mEPSC and mIPSC frequencies in WT and Fabp3 KO mice. C, Plots of the mEPSC and mIPSC amplitudes in WT and Fabp3 KO mice. n = 53 cells for WT and 59 cells for Fabp3 KO from 5 mice for mEPSCs. n = 52 cells for WT and 60 cells for Fabp3 KO from 5 mice for mIPSCs. **p < 0.01 versus WT mice. Error bar indicates mean ± SEM.

    Journal: The Journal of Neuroscience

    Article Title: FABP3 in the Anterior Cingulate Cortex Modulates the Methylation Status of the Glutamic Acid Decarboxylase 67 Promoter Region

    doi: 10.1523/JNEUROSCI.1285-18.2018

    Figure Lengend Snippet: Enhancement of inhibitory synaptic plasticity in the ACC of Fabp3 KO mice. A, Representative traces of mEPSCs and mIPSCs in ACC layer II/III pyramidal neurons. B, Plots of the mEPSC and mIPSC frequencies in WT and Fabp3 KO mice. C, Plots of the mEPSC and mIPSC amplitudes in WT and Fabp3 KO mice. n = 53 cells for WT and 59 cells for Fabp3 KO from 5 mice for mEPSCs. n = 52 cells for WT and 60 cells for Fabp3 KO from 5 mice for mIPSCs. **p < 0.01 versus WT mice. Error bar indicates mean ± SEM.

    Article Snippet: The primary antibodies included mouse monoclonal antibodies against FABP3 (1:200, Hycult Biotechnology HM2016, RRID: AB_533050 ); rabbit polyclonal antibodies against neurogranin (Ng, 1:2000, Millipore AB5620, RRID: AB_91937 ); rabbit polyclonal antibodies against PV (1:2000, Abcam ab11427, RRID: AB_298032 ); rat monoclonal antibodies against somatostatin (SOM, 1:50, Millipore MAB354, RRID: AB_2255365 ); and rabbit polyclonal antibodies against calretinin (CR, 1:2000, Swant CR7697, RRID: AB_2619710 ).

    Techniques:

    Extracellular glutamate (Glu) concentrations in the ACC in freely moving animals. A, Dialysate Glu concentrations at baseline (basal) and after depolarization stimulation (High-K+) (left). Glu AUC before (basal) and after (High-K+) depolarization stimulation (right). *p < 0.05 versus wild-type mice. B, Normalization of the dialysate Glu signals indicated that the response to depolarization stimulation was enhanced in Fabp3 KO mice (left). The normalized Glu AUC after depolarization stimulation (right). A, B, Error bar indicates mean ± SEM. *p < 0.05 versus WT mice. n = 6 WT mice; n = 5 Fabp3 KO mice. C, D, Representative immunoblots (left) and quantitative densitometry analysis (right) probed with various antibodies. *p < 0.05; **p < 0.01 versus WT mice. n = 6 mice per group. Error bar indicates mean ± SEM.

    Journal: The Journal of Neuroscience

    Article Title: FABP3 in the Anterior Cingulate Cortex Modulates the Methylation Status of the Glutamic Acid Decarboxylase 67 Promoter Region

    doi: 10.1523/JNEUROSCI.1285-18.2018

    Figure Lengend Snippet: Extracellular glutamate (Glu) concentrations in the ACC in freely moving animals. A, Dialysate Glu concentrations at baseline (basal) and after depolarization stimulation (High-K+) (left). Glu AUC before (basal) and after (High-K+) depolarization stimulation (right). *p < 0.05 versus wild-type mice. B, Normalization of the dialysate Glu signals indicated that the response to depolarization stimulation was enhanced in Fabp3 KO mice (left). The normalized Glu AUC after depolarization stimulation (right). A, B, Error bar indicates mean ± SEM. *p < 0.05 versus WT mice. n = 6 WT mice; n = 5 Fabp3 KO mice. C, D, Representative immunoblots (left) and quantitative densitometry analysis (right) probed with various antibodies. *p < 0.05; **p < 0.01 versus WT mice. n = 6 mice per group. Error bar indicates mean ± SEM.

    Article Snippet: The primary antibodies included mouse monoclonal antibodies against FABP3 (1:200, Hycult Biotechnology HM2016, RRID: AB_533050 ); rabbit polyclonal antibodies against neurogranin (Ng, 1:2000, Millipore AB5620, RRID: AB_91937 ); rabbit polyclonal antibodies against PV (1:2000, Abcam ab11427, RRID: AB_298032 ); rat monoclonal antibodies against somatostatin (SOM, 1:50, Millipore MAB354, RRID: AB_2255365 ); and rabbit polyclonal antibodies against calretinin (CR, 1:2000, Swant CR7697, RRID: AB_2619710 ).

    Techniques: Western Blot

    Decreased binding of MeCP2 and HDAC1 to the Gad67 promoter region in the ACC of Fabp3 KO mice. A, Representative immunoblots (top) and quantitative densitometry analysis (bottom) probed with various antibodies are shown. n = 6 mice per group. B, ChIP assay of ACC extracts from WT or Fabp3 KO mice via anti-MeCP2 and HDAC1. The precipitated DNA was analyzed by qPCR with primers amplifying the Gad67 promoter region. **p < 0.01 versus WT mice. n = 5 WT mice. n = 4 Fabp3 KO mice. C, DNA methylation profile in the Gad67 promoter region determined by bisulfite sequencing. Black circles represent methylated CpGs. Open circles represent unmethylated sites. Top numbers indicate global percentages of methylated cytosines. D, Quantitative analysis of the Gad67 mRNA expression in the ACC. WT, n = 6; KO, n = 5; KO + MET, n = 6 mice per group. **p < 0.01 versus WT mice. ##p < 0.01 versus KO mice. E, F, Quantitative analysis of the SAM concentration by ELISA. n = 4 per group. **p < 0.01 versus mock control. MET treatment (5.2 mmol/kg, s.c., twice per day for 6 d). mock, Mock control; OE, Fabp3 overexpression. Error bar indicates mean ± SEM.

    Journal: The Journal of Neuroscience

    Article Title: FABP3 in the Anterior Cingulate Cortex Modulates the Methylation Status of the Glutamic Acid Decarboxylase 67 Promoter Region

    doi: 10.1523/JNEUROSCI.1285-18.2018

    Figure Lengend Snippet: Decreased binding of MeCP2 and HDAC1 to the Gad67 promoter region in the ACC of Fabp3 KO mice. A, Representative immunoblots (top) and quantitative densitometry analysis (bottom) probed with various antibodies are shown. n = 6 mice per group. B, ChIP assay of ACC extracts from WT or Fabp3 KO mice via anti-MeCP2 and HDAC1. The precipitated DNA was analyzed by qPCR with primers amplifying the Gad67 promoter region. **p < 0.01 versus WT mice. n = 5 WT mice. n = 4 Fabp3 KO mice. C, DNA methylation profile in the Gad67 promoter region determined by bisulfite sequencing. Black circles represent methylated CpGs. Open circles represent unmethylated sites. Top numbers indicate global percentages of methylated cytosines. D, Quantitative analysis of the Gad67 mRNA expression in the ACC. WT, n = 6; KO, n = 5; KO + MET, n = 6 mice per group. **p < 0.01 versus WT mice. ##p < 0.01 versus KO mice. E, F, Quantitative analysis of the SAM concentration by ELISA. n = 4 per group. **p < 0.01 versus mock control. MET treatment (5.2 mmol/kg, s.c., twice per day for 6 d). mock, Mock control; OE, Fabp3 overexpression. Error bar indicates mean ± SEM.

    Article Snippet: The primary antibodies included mouse monoclonal antibodies against FABP3 (1:200, Hycult Biotechnology HM2016, RRID: AB_533050 ); rabbit polyclonal antibodies against neurogranin (Ng, 1:2000, Millipore AB5620, RRID: AB_91937 ); rabbit polyclonal antibodies against PV (1:2000, Abcam ab11427, RRID: AB_298032 ); rat monoclonal antibodies against somatostatin (SOM, 1:50, Millipore MAB354, RRID: AB_2255365 ); and rabbit polyclonal antibodies against calretinin (CR, 1:2000, Swant CR7697, RRID: AB_2619710 ).

    Techniques: Binding Assay, Western Blot, DNA Methylation Assay, Methylation Sequencing, Methylation, Expressing, Concentration Assay, Enzyme-linked Immunosorbent Assay, Over Expression

    Amelioration of behavioral abnormalities in Fabp3 KO mice by chronic treatment with MET. A, Schematic of the behavioral tests and MET treatment (5.2 mmol/kg, s.c., twice per day). During the behavioral testing period, mice received saline or MET once per day after testing. B, OFT. Graph represents the time spent in the center area over a 5 min period for WT and Fabp3 KO mice. n = 14–17 mice per group. C, HBT. An illustrative example of the travel pathway in the HBT using video tracking software (top). Graphs represent the time spent in the center area, total number of head-dips, head-dip duration, and latency to first head-dip for the 5 min testing session (bottom). n = 13–15 mice per group. **p < 0.01 versus saline-treated WT mice. ##p < 0.01 versus saline-treated KO mice. D, NORT. Differences in exploratory preference were assessed between groups in the training (left) or test (right) sessions. n = 11–15 mice per group. **p < 0.01 versus familiar group. Sal, Saline treatment. MET treatment (5.2 mmol/kg, s.c., twice per day for 6 d). Error bar indicates mean ± SEM.

    Journal: The Journal of Neuroscience

    Article Title: FABP3 in the Anterior Cingulate Cortex Modulates the Methylation Status of the Glutamic Acid Decarboxylase 67 Promoter Region

    doi: 10.1523/JNEUROSCI.1285-18.2018

    Figure Lengend Snippet: Amelioration of behavioral abnormalities in Fabp3 KO mice by chronic treatment with MET. A, Schematic of the behavioral tests and MET treatment (5.2 mmol/kg, s.c., twice per day). During the behavioral testing period, mice received saline or MET once per day after testing. B, OFT. Graph represents the time spent in the center area over a 5 min period for WT and Fabp3 KO mice. n = 14–17 mice per group. C, HBT. An illustrative example of the travel pathway in the HBT using video tracking software (top). Graphs represent the time spent in the center area, total number of head-dips, head-dip duration, and latency to first head-dip for the 5 min testing session (bottom). n = 13–15 mice per group. **p < 0.01 versus saline-treated WT mice. ##p < 0.01 versus saline-treated KO mice. D, NORT. Differences in exploratory preference were assessed between groups in the training (left) or test (right) sessions. n = 11–15 mice per group. **p < 0.01 versus familiar group. Sal, Saline treatment. MET treatment (5.2 mmol/kg, s.c., twice per day for 6 d). Error bar indicates mean ± SEM.

    Article Snippet: The primary antibodies included mouse monoclonal antibodies against FABP3 (1:200, Hycult Biotechnology HM2016, RRID: AB_533050 ); rabbit polyclonal antibodies against neurogranin (Ng, 1:2000, Millipore AB5620, RRID: AB_91937 ); rabbit polyclonal antibodies against PV (1:2000, Abcam ab11427, RRID: AB_298032 ); rat monoclonal antibodies against somatostatin (SOM, 1:50, Millipore MAB354, RRID: AB_2255365 ); and rabbit polyclonal antibodies against calretinin (CR, 1:2000, Swant CR7697, RRID: AB_2619710 ).

    Techniques: Software

    Locomotor activities during behavioral tests a

    Journal: The Journal of Neuroscience

    Article Title: FABP3 in the Anterior Cingulate Cortex Modulates the Methylation Status of the Glutamic Acid Decarboxylase 67 Promoter Region

    doi: 10.1523/JNEUROSCI.1285-18.2018

    Figure Lengend Snippet: Locomotor activities during behavioral tests a

    Article Snippet: The primary antibodies included mouse monoclonal antibodies against FABP3 (1:200, Hycult Biotechnology HM2016, RRID: AB_533050 ); rabbit polyclonal antibodies against neurogranin (Ng, 1:2000, Millipore AB5620, RRID: AB_91937 ); rabbit polyclonal antibodies against PV (1:2000, Abcam ab11427, RRID: AB_298032 ); rat monoclonal antibodies against somatostatin (SOM, 1:50, Millipore MAB354, RRID: AB_2255365 ); and rabbit polyclonal antibodies against calretinin (CR, 1:2000, Swant CR7697, RRID: AB_2619710 ).

    Techniques: